Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75105.t1 | KEH27276.1 | 98.2 | 56 | 1 | 0 | 1 | 56 | 1 | 56 | 1.60E-23 | 118.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75105.t1 | A0A072UCT8 | 98.2 | 56 | 1 | 0 | 1 | 56 | 1 | 56 | 1.1e-23 | 118.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050838 | MS.gene75105 | 0.814524 | 1.47E-51 | -1.69E-46 |
| MS.gene051746 | MS.gene75105 | 0.835838 | 1.33E-56 | -1.69E-46 |
| MS.gene051748 | MS.gene75105 | 0.84624 | 2.46E-59 | -1.69E-46 |
| MS.gene051850 | MS.gene75105 | 0.814179 | 1.76E-51 | -1.69E-46 |
| MS.gene053109 | MS.gene75105 | 0.821116 | 4.79E-53 | -1.69E-46 |
| MS.gene053952 | MS.gene75105 | 0.801885 | 7.29E-49 | -1.69E-46 |
| MS.gene056432 | MS.gene75105 | 0.802279 | 6.05E-49 | -1.69E-46 |
| MS.gene057094 | MS.gene75105 | 0.814332 | 1.63E-51 | -1.69E-46 |
| MS.gene057234 | MS.gene75105 | 0.81728 | 3.58E-52 | -1.69E-46 |
| MS.gene058055 | MS.gene75105 | 0.806775 | 6.98E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75105.t1 | MTR_6g093010 | 84.848 | 66 | 10 | 0 | 1 | 66 | 1 | 66 | 6.99e-36 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75105.t1 | AT2G31490 | 73.770 | 61 | 16 | 0 | 1 | 61 | 1 | 61 | 2.36e-30 | 102 |
Find 19 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTCTCGTTGGTATCTT+CGG | 0.346812 | 6.3:-84348413 | MS.gene75105:CDS |
| AAACAAATACATTGAAGATT+GGG | 0.354310 | 6.3:-84348486 | MS.gene75105:CDS |
| TTGCGATGTTGGAGCATAGA+TGG | 0.385992 | 6.3:-84346745 | MS.gene75105:CDS |
| AAAACAAATACATTGAAGAT+TGG | 0.399418 | 6.3:-84348487 | MS.gene75105:CDS |
| ATGCCTTTGTAGACGAGAAC+AGG | 0.444933 | 6.3:+84348380 | None:intergenic |
| CGTCGCAATCTTCTTCTCGT+TGG | 0.495434 | 6.3:-84348422 | MS.gene75105:CDS |
| ATCTTGAACACAATTTCCGT+TGG | 0.511606 | 6.3:-84348448 | MS.gene75105:CDS |
| ATCGAAAATGGGTGGAGGAA+TGG | 0.519119 | 6.3:-84348519 | None:intergenic |
| TTGAAGATTGGGGAACTGCT+AGG | 0.519133 | 6.3:-84348475 | MS.gene75105:CDS |
| GAATTTAAAGTTTGCGATGT+TGG | 0.525831 | 6.3:-84346756 | MS.gene75105:intron |
| CCAATTTACTACAGCTAACA+AGG | 0.533803 | 6.3:+84346701 | None:intergenic |
| TGAAGATTGGGGAACTGCTA+GGG | 0.554642 | 6.3:-84348474 | MS.gene75105:CDS |
| TGCGATGTTGGAGCATAGAT+GGG | 0.574819 | 6.3:-84346744 | MS.gene75105:CDS |
| GAAGATTGCGACGAGACCAA+CGG | 0.577698 | 6.3:+84348432 | None:intergenic |
| AACAAATACATTGAAGATTG+GGG | 0.581008 | 6.3:-84348485 | MS.gene75105:CDS |
| TCATCATCGAAAATGGGTGG+AGG | 0.588259 | 6.3:-84348524 | None:intergenic |
| GTTCCTGTTCTCGTCTACAA+AGG | 0.608218 | 6.3:-84348383 | MS.gene75105:CDS |
| TCATCATCATCGAAAATGGG+TGG | 0.630058 | 6.3:-84348527 | None:intergenic |
| AACAAGGTCTGTAGACAGTG+CGG | 0.705055 | 6.3:+84346717 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACTTATTTTCAATTGTTTT+GGG | - | chr6.3:84347106-84347125 | MS.gene75105:intron | 15.0% |
| !!! | TGTTTTATTATAAGCTATTT+TGG | - | chr6.3:84347936-84347955 | MS.gene75105:intron | 15.0% |
| !! | AAAAAATAAGTCAATCCAAA+AGG | + | chr6.3:84347133-84347152 | None:intergenic | 20.0% |
| !! | AAAACAAATACATTGAAGAT+TGG | - | chr6.3:84346740-84346759 | MS.gene75105:CDS | 20.0% |
| !! | AAACAAATACATTGAAGATT+GGG | - | chr6.3:84346741-84346760 | MS.gene75105:CDS | 20.0% |
| !! | ATTTCTGAATAGTTCAATTT+AGG | - | chr6.3:84346929-84346948 | MS.gene75105:intron | 20.0% |
| !! | GAAAAATTGTGTTTATCTAA+GGG | + | chr6.3:84347313-84347332 | None:intergenic | 20.0% |
| !! | TATTAACAACTCAGAATTTA+GGG | - | chr6.3:84347554-84347573 | MS.gene75105:intron | 20.0% |
| !! | TGAAAAATTGTGTTTATCTA+AGG | + | chr6.3:84347314-84347333 | None:intergenic | 20.0% |
| !! | TTATTAACAACTCAGAATTT+AGG | - | chr6.3:84347553-84347572 | MS.gene75105:intron | 20.0% |
| !!! | AACACATTTTAGCATTATTT+TGG | - | chr6.3:84347365-84347384 | MS.gene75105:intron | 20.0% |
| !!! | ACACATTTTAGCATTATTTT+GGG | - | chr6.3:84347366-84347385 | MS.gene75105:intron | 20.0% |
| !!! | CAACTTATTTTCAATTGTTT+TGG | - | chr6.3:84347105-84347124 | MS.gene75105:intron | 20.0% |
| !!! | CAATTTTTACAAGCTTTTTT+CGG | + | chr6.3:84347980-84347999 | None:intergenic | 20.0% |
| ! | AACAAATACATTGAAGATTG+GGG | - | chr6.3:84346742-84346761 | MS.gene75105:CDS | 25.0% |
| ! | AACAGTTCAAAAGAATTTAG+GGG | - | chr6.3:84347612-84347631 | MS.gene75105:intron | 25.0% |
| ! | ACAACAGTTCAAAAGAATTT+AGG | - | chr6.3:84347610-84347629 | MS.gene75105:intron | 25.0% |
| ! | AGTTCAATTTAGGAATTTGA+GGG | - | chr6.3:84346939-84346958 | MS.gene75105:intron | 25.0% |
| ! | CAACAGTTCAAAAGAATTTA+GGG | - | chr6.3:84347611-84347630 | MS.gene75105:intron | 25.0% |
| ! | CAATCTAAACAAATTGAGTA+TGG | - | chr6.3:84348323-84348342 | MS.gene75105:intron | 25.0% |
| ! | CAGAATTTAGGGATTTAATT+AGG | - | chr6.3:84347565-84347584 | MS.gene75105:intron | 25.0% |
| ! | GTTGTTAATAAATTGACCAA+TGG | + | chr6.3:84347544-84347563 | None:intergenic | 25.0% |
| ! | TAAATGCTTGCTTGAATTAT+TGG | - | chr6.3:84348374-84348393 | MS.gene75105:CDS | 25.0% |
| ! | TAGTTCAATTTAGGAATTTG+AGG | - | chr6.3:84346938-84346957 | MS.gene75105:intron | 25.0% |
| ! | TGATTATTTCAATCTACCAT+TGG | - | chr6.3:84347525-84347544 | MS.gene75105:intron | 25.0% |
| ! | TTAATGCTTGCTTGAATTAT+TGG | - | chr6.3:84348430-84348449 | MS.gene75105:CDS | 25.0% |
| ! | TTGGTTGTAATGTTTAATTG+TGG | - | chr6.3:84348393-84348412 | MS.gene75105:CDS | 25.0% |
| !!! | AAAGTTTCTTACTTTTTCTG+TGG | - | chr6.3:84346985-84347004 | MS.gene75105:intron | 25.0% |
| !!! | AAGAATTTAGGGGTTTTATT+AGG | - | chr6.3:84347622-84347641 | MS.gene75105:intron | 25.0% |
| !!! | AAGTTTCTTACTTTTTCTGT+GGG | - | chr6.3:84346986-84347005 | MS.gene75105:intron | 25.0% |
| AAAAATAAGCCAATCCAAAC+TGG | - | chr6.3:84347287-84347306 | MS.gene75105:intron | 30.0% | |
| AAAGCTGGAAATCCAAATTT+AGG | + | chr6.3:84348275-84348294 | None:intergenic | 30.0% | |
| AAGAGAGAAGAAGATGAAAA+TGG | + | chr6.3:84346897-84346916 | None:intergenic | 30.0% | |
| ATCCAAACAAGTCCTAAATT+TGG | - | chr6.3:84348260-84348279 | MS.gene75105:intron | 30.0% | |
| CTTACATAAGCTTATAGCAT+GGG | + | chr6.3:84347881-84347900 | None:intergenic | 30.0% | |
| GCAAATAATTAAGCTTGTCA+AGG | - | chr6.3:84347160-84347179 | MS.gene75105:intron | 30.0% | |
| ! | AAATTTGGATTTCCAGCTTT+TGG | - | chr6.3:84348275-84348294 | MS.gene75105:intron | 30.0% |
| ! | ATCCAAATTTAGGACTTGTT+TGG | + | chr6.3:84348265-84348284 | None:intergenic | 30.0% |
| ! | GAATTTAAAGTTTGCGATGT+TGG | - | chr6.3:84348471-84348490 | MS.gene75105:CDS | 30.0% |
| !! | ATAAGTTTTGTGAGCTTGAT+TGG | - | chr6.3:84347036-84347055 | MS.gene75105:intron | 30.0% |
| !! | ATTTTGATCAAGTCTGTGTT+TGG | - | chr6.3:84347804-84347823 | MS.gene75105:intron | 30.0% |
| !! | TTGTTATGAATGCATTGACT+TGG | - | chr6.3:84347773-84347792 | MS.gene75105:intron | 30.0% |
| ATCTTGAACACAATTTCCGT+TGG | - | chr6.3:84346779-84346798 | MS.gene75105:intron | 35.0% | |
| GAAGAAGATGAAAATGGAAG+TGG | + | chr6.3:84346891-84346910 | None:intergenic | 35.0% | |
| GCTTACATAAGCTTATAGCA+TGG | + | chr6.3:84347882-84347901 | None:intergenic | 35.0% | |
| TCAAATAAGCCAATCCAAAC+AGG | - | chr6.3:84348062-84348081 | MS.gene75105:intron | 35.0% | |
| TCAATTTAGGAATTTGAGGG+AGG | - | chr6.3:84346942-84346961 | MS.gene75105:intron | 35.0% | |
| !! | AACTGTATTTTGAGCCTGTT+TGG | + | chr6.3:84348079-84348098 | None:intergenic | 35.0% |
| !! | TATTTTGAGCCTGTTTGGAT+TGG | + | chr6.3:84348074-84348093 | None:intergenic | 35.0% |
| !!! | AGCATTATTTTGGGTCTGTT+AGG | - | chr6.3:84347375-84347394 | MS.gene75105:intron | 35.0% |
| !!! | TCAATTGTTTTGGGACCTTT+TGG | - | chr6.3:84347115-84347134 | MS.gene75105:intron | 35.0% |
| CTACTAACATAAGCACCTGT+GGG | + | chr6.3:84348222-84348241 | None:intergenic | 40.0% | |
| CTTCTTCTCGTTGGTATCTT+CGG | - | chr6.3:84346814-84346833 | MS.gene75105:intron | 40.0% | |
| GCTTATTTGAGCTTACCAAC+TGG | + | chr6.3:84348052-84348071 | None:intergenic | 40.0% | |
| TCTACTAACATAAGCACCTG+TGG | + | chr6.3:84348223-84348242 | None:intergenic | 40.0% | |
| TGTTTATCTAAGGGCCAGTT+TGG | + | chr6.3:84347304-84347323 | None:intergenic | 40.0% | |
| TTGGGTCTGTTAGGATAAAC+AGG | - | chr6.3:84347384-84347403 | MS.gene75105:intron | 40.0% | |
| TTTGCACCTTATAGCACAAG+CGG | - | chr6.3:84347840-84347859 | MS.gene75105:intron | 40.0% | |
| ! | CATTAACCGATTCCAAAAGC+TGG | + | chr6.3:84348290-84348309 | None:intergenic | 40.0% |
| !!! | GGATTTCCAGCTTTTGGAAT+CGG | - | chr6.3:84348281-84348300 | MS.gene75105:intron | 40.0% |
| AACAAGGTCTGTAGACAGTG+CGG | + | chr6.3:84348513-84348532 | None:intergenic | 45.0% | |
| ATCTAAGGGCCAGTTTGGAT+TGG | + | chr6.3:84347299-84347318 | None:intergenic | 45.0% | |
| ATGCCTTTGTAGACGAGAAC+AGG | + | chr6.3:84346850-84346869 | None:intergenic | 45.0% | |
| CTCATGACGCAACATTGTGA+TGG | + | chr6.3:84347694-84347713 | None:intergenic | 45.0% | |
| GGAGGTTCTACTACGTGATT+TGG | - | chr6.3:84346960-84346979 | MS.gene75105:intron | 45.0% | |
| GTTCCTGTTCTCGTCTACAA+AGG | - | chr6.3:84346844-84346863 | MS.gene75105:intron | 45.0% | |
| TGAAGATTGGGGAACTGCTA+GGG | - | chr6.3:84346753-84346772 | MS.gene75105:intron | 45.0% | |
| TGCGATGTTGGAGCATAGAT+GGG | - | chr6.3:84348483-84348502 | MS.gene75105:CDS | 45.0% | |
| TTGAAGATTGGGGAACTGCT+AGG | - | chr6.3:84346752-84346771 | MS.gene75105:intron | 45.0% | |
| TTGCGATGTTGGAGCATAGA+TGG | - | chr6.3:84348482-84348501 | MS.gene75105:CDS | 45.0% | |
| !!! | ATTTTATTTAAGTTTATATA+TGG | - | chr6.3:84347011-84347030 | MS.gene75105:intron | 5.0% |
| AGTTCTCCAAACAGTCCCAC+AGG | - | chr6.3:84348204-84348223 | MS.gene75105:intron | 50.0% | |
| CGACAACCGCTTGTGCTATA+AGG | + | chr6.3:84347849-84347868 | None:intergenic | 50.0% | |
| CGTCGCAATCTTCTTCTCGT+TGG | - | chr6.3:84346805-84346824 | MS.gene75105:intron | 50.0% | |
| CTCACAAGACTTGTGCCAGT+TGG | - | chr6.3:84348034-84348053 | MS.gene75105:intron | 50.0% | |
| GAAGATTGCGACGAGACCAA+CGG | + | chr6.3:84346798-84346817 | None:intergenic | 50.0% | |
| TAAGCACCTGTGGGACTGTT+TGG | + | chr6.3:84348213-84348232 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 84346715 | 84348534 | 84346715 | ID=MS.gene75105 |
| chr6.3 | mRNA | 84346715 | 84348534 | 84346715 | ID=MS.gene75105.t1;Parent=MS.gene75105 |
| chr6.3 | exon | 84348367 | 84348534 | 84348367 | ID=MS.gene75105.t1.exon1;Parent=MS.gene75105.t1 |
| chr6.3 | CDS | 84348367 | 84348534 | 84348367 | ID=cds.MS.gene75105.t1;Parent=MS.gene75105.t1 |
| chr6.3 | exon | 84346715 | 84346768 | 84346715 | ID=MS.gene75105.t1.exon2;Parent=MS.gene75105.t1 |
| chr6.3 | CDS | 84346715 | 84346768 | 84346715 | ID=cds.MS.gene75105.t1;Parent=MS.gene75105.t1 |
| Gene Sequence |
| Protein sequence |
