Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75597.t1 | XP_003601799.1 | 98.9 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 7.70E-44 | 186 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75597.t1 | Q944J0 | 71.6 | 88 | 25 | 0 | 1 | 88 | 1 | 88 | 6.4e-31 | 134.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75597.t1 | G7JC07 | 98.9 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 5.6e-44 | 186.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene75597 | MS.gene89036 | PPI |
| MS.gene75597 | MS.gene36215 | PPI |
| MS.gene75597 | MS.gene86381 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75597.t1 | MTR_3g085510 | 98.925 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 2.23e-64 | 189 |
| MS.gene75597.t1 | MTR_8g080380 | 78.495 | 93 | 20 | 0 | 1 | 93 | 92 | 184 | 3.90e-52 | 161 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75597.t1 | AT4G40042 | 79.747 | 79 | 16 | 0 | 1 | 79 | 1 | 79 | 1.26e-43 | 137 |
| MS.gene75597.t1 | AT2G22425 | 71.591 | 88 | 25 | 0 | 1 | 88 | 1 | 88 | 1.76e-41 | 131 |
| MS.gene75597.t1 | AT2G22425 | 71.591 | 88 | 25 | 0 | 1 | 88 | 1 | 88 | 1.76e-41 | 131 |
Find 26 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTTACCCTACTTCTTAA+TGG | 0.179148 | 3.3:-71365322 | None:intergenic |
| GAGAGGATGGCGGTTGAAAA+AGG | 0.272451 | 3.3:-71365223 | None:intergenic |
| CATTGGTGACTGTCCCAAAT+TGG | 0.297025 | 3.3:+71365199 | MS.gene75597:CDS |
| TCTTTACCCTACTTCTTAAT+GGG | 0.300035 | 3.3:-71365321 | None:intergenic |
| GCATTTGCTGTGGTTGCATT+TGG | 0.308000 | 3.3:+71365108 | MS.gene75597:CDS |
| TTCTTCTTTGAATTCACAAT+AGG | 0.417935 | 3.3:-71365297 | None:intergenic |
| GAAGAAACCCATTAAGAAGT+AGG | 0.438658 | 3.3:+71365314 | MS.gene75597:CDS |
| TCTGCATTAGCTTCTCTGCT+AGG | 0.440951 | 3.3:-71365074 | None:intergenic |
| ATAGGAGATAGCTGTGGCTT+CGG | 0.450784 | 3.3:-71365279 | None:intergenic |
| GCTGTGGTTGCATTTGGAAC+GGG | 0.459654 | 3.3:+71365114 | MS.gene75597:CDS |
| TGCTGTGGTTGCATTTGGAA+CGG | 0.510809 | 3.3:+71365113 | MS.gene75597:CDS |
| AAGAAACCCATTAAGAAGTA+GGG | 0.531311 | 3.3:+71365315 | MS.gene75597:CDS |
| TTCATCAGATGGATTGGCAA+GGG | 0.536217 | 3.3:+71365046 | None:intergenic |
| CCGCCATCCTCTCAAGTGGT+TGG | 0.536748 | 3.3:+71365233 | MS.gene75597:CDS |
| TCAACCGCCATCCTCTCAAG+TGG | 0.553865 | 3.3:+71365229 | MS.gene75597:CDS |
| GATCCAACCACTTGAGAGGA+TGG | 0.566183 | 3.3:-71365236 | None:intergenic |
| CTTCATCAGATGGATTGGCA+AGG | 0.566563 | 3.3:+71365045 | None:intergenic |
| CTTGGATCCAACCACTTGAG+AGG | 0.567895 | 3.3:-71365240 | None:intergenic |
| AATGTTGCTTGCATTTGCTG+TGG | 0.601891 | 3.3:+71365098 | MS.gene75597:CDS |
| ACGATGATGCTCATATATGC+TGG | 0.624836 | 3.3:+71365159 | MS.gene75597:CDS |
| TGGTGTTGTTCTCACCACAT+TGG | 0.662379 | 3.3:+71365182 | MS.gene75597:CDS |
| CAAGTGGTTGGATCCAAGTG+AGG | 0.664517 | 3.3:+71365245 | MS.gene75597:CDS |
| ATGATGCTCATATATGCTGG+TGG | 0.699379 | 3.3:+71365162 | MS.gene75597:CDS |
| CCAACCACTTGAGAGGATGG+CGG | 0.730520 | 3.3:-71365233 | None:intergenic |
| ATTTGGGACAGTCACCAATG+TGG | 0.730637 | 3.3:-71365196 | None:intergenic |
| TTCACAATAGGAGATAGCTG+TGG | 0.732645 | 3.3:-71365285 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCTTCTTTGAATTCACAAT+AGG | - | chr3.3:71365300-71365319 | None:intergenic | 25.0% |
| ACGATGATGCTCATATATGC+TGG | + | chr3.3:71365159-71365178 | MS.gene75597:CDS | 40.0% | |
| ATGATGCTCATATATGCTGG+TGG | + | chr3.3:71365162-71365181 | MS.gene75597:CDS | 40.0% | |
| GGTTGAAAAAGGGCCAATTT+GGG | - | chr3.3:71365215-71365234 | None:intergenic | 40.0% | |
| TTCACAATAGGAGATAGCTG+TGG | - | chr3.3:71365288-71365307 | None:intergenic | 40.0% | |
| ! | AATGTTGCTTGCATTTGCTG+TGG | + | chr3.3:71365098-71365117 | MS.gene75597:CDS | 40.0% |
| ATAGGAGATAGCTGTGGCTT+CGG | - | chr3.3:71365282-71365301 | None:intergenic | 45.0% | |
| CGGTTGAAAAAGGGCCAATT+TGG | - | chr3.3:71365216-71365235 | None:intergenic | 45.0% | |
| TGCTGTGGTTGCATTTGGAA+CGG | + | chr3.3:71365113-71365132 | MS.gene75597:CDS | 45.0% | |
| ! | AGAGGATGGCGGTTGAAAAA+GGG | - | chr3.3:71365225-71365244 | None:intergenic | 45.0% |
| ! | ATTTGGGACAGTCACCAATG+TGG | - | chr3.3:71365199-71365218 | None:intergenic | 45.0% |
| ! | CATTGGTGACTGTCCCAAAT+TGG | + | chr3.3:71365199-71365218 | MS.gene75597:CDS | 45.0% |
| ! | GGATGCTTTTCTACCTCACT+TGG | - | chr3.3:71365261-71365280 | None:intergenic | 45.0% |
| ! | TCTGCATTAGCTTCTCTGCT+AGG | - | chr3.3:71365077-71365096 | None:intergenic | 45.0% |
| !! | GCATTTGCTGTGGTTGCATT+TGG | + | chr3.3:71365108-71365127 | MS.gene75597:CDS | 45.0% |
| !! | TGGTGTTGTTCTCACCACAT+TGG | + | chr3.3:71365182-71365201 | MS.gene75597:CDS | 45.0% |
| CAAGTGGTTGGATCCAAGTG+AGG | + | chr3.3:71365245-71365264 | MS.gene75597:CDS | 50.0% | |
| CTTGGATCCAACCACTTGAG+AGG | - | chr3.3:71365243-71365262 | None:intergenic | 50.0% | |
| GATCCAACCACTTGAGAGGA+TGG | - | chr3.3:71365239-71365258 | None:intergenic | 50.0% | |
| ! | GAGAGGATGGCGGTTGAAAA+AGG | - | chr3.3:71365226-71365245 | None:intergenic | 50.0% |
| ! | GCTGTGGTTGCATTTGGAAC+GGG | + | chr3.3:71365114-71365133 | MS.gene75597:CDS | 50.0% |
| CCAACCACTTGAGAGGATGG+CGG | - | chr3.3:71365236-71365255 | None:intergenic | 55.0% | |
| TCAACCGCCATCCTCTCAAG+TGG | + | chr3.3:71365229-71365248 | MS.gene75597:CDS | 55.0% | |
| CCGCCATCCTCTCAAGTGGT+TGG | + | chr3.3:71365233-71365252 | MS.gene75597:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 71365054 | 71365335 | 71365054 | ID=MS.gene75597 |
| chr3.3 | mRNA | 71365054 | 71365335 | 71365054 | ID=MS.gene75597.t1;Parent=MS.gene75597 |
| chr3.3 | exon | 71365054 | 71365335 | 71365054 | ID=MS.gene75597.t1.exon1;Parent=MS.gene75597.t1 |
| chr3.3 | CDS | 71365054 | 71365335 | 71365054 | ID=cds.MS.gene75597.t1;Parent=MS.gene75597.t1 |
| Gene Sequence |
| Protein sequence |
