AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75760.t1 MTR_3g109160 100.000 103 0 0 1 103 57 159 6.59e-74 216
MS.gene75760.t1 MTR_1g009150 71.681 113 22 4 1 103 55 167 5.68e-50 156
MS.gene75760.t1 MTR_4g021810 68.478 92 22 3 1 85 10 101 1.42e-34 114
MS.gene75760.t1 MTR_4g005320 69.565 69 21 0 11 79 68 136 3.80e-31 108
MS.gene75760.t1 MTR_2g043960 55.072 69 31 0 13 81 76 144 6.21e-23 87.0
MS.gene75760.t1 MTR_4g124750 56.923 65 28 0 13 77 69 133 5.24e-21 82.0
MS.gene75760.t1 MTR_1g024085 39.785 93 48 2 13 97 37 129 6.46e-17 71.2
MS.gene75760.t1 MTR_4g072570 45.570 79 42 1 1 78 13 91 6.67e-17 69.7
MS.gene75760.t1 MTR_3g084240 53.731 67 30 1 13 78 25 91 1.90e-16 68.6
MS.gene75760.t1 MTR_4g072190 46.875 64 34 0 13 76 36 99 2.27e-16 68.6
MS.gene75760.t1 MTR_3g084170 53.731 67 30 1 13 78 25 91 2.36e-16 68.2
MS.gene75760.t1 MTR_4g050700 52.239 67 31 1 13 78 26 92 3.14e-16 68.2
MS.gene75760.t1 MTR_2g012410 52.239 67 31 1 13 78 26 92 3.14e-16 68.2
MS.gene75760.t1 MTR_4g072160 50.685 73 35 1 13 84 37 109 3.92e-16 68.9
MS.gene75760.t1 MTR_3g117610 46.875 64 34 0 13 76 45 108 5.37e-16 67.8
MS.gene75760.t1 MTR_4g072880 49.254 67 33 1 13 78 26 92 8.09e-16 67.0
MS.gene75760.t1 MTR_4g072330 54.098 61 27 1 13 72 23 83 1.33e-15 66.2
MS.gene75760.t1 MTR_4g072370 54.098 61 27 1 13 72 23 83 1.54e-15 66.2
MS.gene75760.t1 MTR_8g461210 46.970 66 35 0 11 76 33 98 1.74e-15 66.6
MS.gene75760.t1 MTR_3g084150 50.746 67 32 1 13 78 25 91 1.74e-15 66.2
MS.gene75760.t1 MTR_3g084180 50.746 67 32 1 13 78 25 91 1.90e-15 65.9
MS.gene75760.t1 MTR_1g059770 42.029 69 40 0 9 77 45 113 2.65e-15 66.6
MS.gene75760.t1 MTR_8g026730 40.789 76 43 1 12 85 26 101 2.65e-15 66.2
MS.gene75760.t1 MTR_4g072690 53.226 62 28 1 12 72 19 80 3.77e-15 65.1
MS.gene75760.t1 MTR_5g021820 44.928 69 38 0 8 76 35 103 4.41e-15 65.5
MS.gene75760.t1 MTR_2g066830 45.455 66 36 0 12 77 57 122 4.99e-15 66.2
MS.gene75760.t1 MTR_8g461410 49.254 67 33 1 13 78 25 91 5.34e-15 64.7
MS.gene75760.t1 MTR_4g072910 57.377 61 25 1 13 72 35 95 5.43e-15 65.1
MS.gene75760.t1 MTR_3g110042 55.738 61 26 1 13 72 25 85 6.21e-15 64.7
MS.gene75760.t1 MTR_4g072660 50.746 67 32 1 13 78 20 86 7.20e-15 64.3
MS.gene75760.t1 MTR_3g084220 50.746 67 32 1 13 78 25 91 9.20e-15 64.3
MS.gene75760.t1 MTR_4g072480 43.038 79 44 1 1 78 13 91 1.12e-14 63.9
MS.gene75760.t1 MTR_4g072250 52.239 67 30 2 13 78 26 91 1.21e-14 63.9
MS.gene75760.t1 MTR_7g405740 43.590 78 42 1 13 90 67 142 1.22e-14 65.9
MS.gene75760.t1 MTR_5g008370 50.746 67 32 1 13 78 25 91 1.33e-14 65.1
MS.gene75760.t1 MTR_4g072220 52.459 61 28 1 13 72 26 86 1.52e-14 63.9
MS.gene75760.t1 MTR_4g072240 50.820 61 29 1 13 72 26 86 1.56e-14 63.5
MS.gene75760.t1 MTR_7g118010 47.059 68 34 1 12 77 22 89 1.61e-14 63.9
MS.gene75760.t1 MTR_4g072940 54.098 61 27 1 13 72 57 117 1.91e-14 64.3
MS.gene75760.t1 MTR_4g072870 47.826 69 35 1 2 69 15 83 1.92e-14 63.5
MS.gene75760.t1 MTR_4g072680 41.772 79 45 1 1 78 13 91 1.97e-14 63.5
MS.gene75760.t1 MTR_3g092220 45.070 71 37 1 9 77 29 99 1.99e-14 63.9
MS.gene75760.t1 MTR_4g072610 47.887 71 36 1 3 72 9 79 2.04e-14 63.2
MS.gene75760.t1 MTR_4g072740 47.887 71 36 1 3 72 10 80 2.12e-14 63.2
MS.gene75760.t1 MTR_4g072460 41.772 79 45 1 1 78 13 91 2.15e-14 63.2
MS.gene75760.t1 MTR_3g113310 45.312 64 35 0 13 76 42 105 2.35e-14 63.5
MS.gene75760.t1 MTR_3g098970 37.234 94 52 3 13 100 53 145 2.41e-14 64.7
MS.gene75760.t1 MTR_4g072640 47.826 69 35 1 5 72 17 85 2.88e-14 63.2
MS.gene75760.t1 MTR_4g072730 47.761 67 34 1 13 78 25 91 3.46e-14 62.8
MS.gene75760.t1 MTR_4g072620 41.772 79 45 1 1 78 13 91 3.53e-14 62.8
MS.gene75760.t1 MTR_4g072650 52.459 61 28 1 13 72 19 79 3.67e-14 62.4
MS.gene75760.t1 MTR_4g072500 49.254 67 33 1 13 78 25 91 3.69e-14 62.8
MS.gene75760.t1 MTR_7g104940 45.161 62 34 0 16 77 53 114 4.61e-14 63.5
MS.gene75760.t1 MTR_4g072270 47.761 67 34 1 13 78 26 92 4.86e-14 62.4
MS.gene75760.t1 MTR_3g110038 54.098 61 27 1 13 72 25 85 4.90e-14 62.4
MS.gene75760.t1 MTR_3g084250 55.172 58 25 1 13 69 25 82 5.06e-14 62.4
MS.gene75760.t1 MTR_4g072470 45.070 71 38 1 3 72 10 80 5.64e-14 62.0
MS.gene75760.t1 MTR_4g072260 53.448 58 26 1 13 69 26 83 6.58e-14 62.0
MS.gene75760.t1 MTR_4g072310 47.761 67 34 1 13 78 26 92 6.72e-14 62.0
MS.gene75760.t1 MTR_4g072720 52.459 61 28 1 13 72 21 81 7.76e-14 61.6
MS.gene75760.t1 MTR_4g072890 56.604 53 22 1 13 64 26 78 8.68e-14 61.6
MS.gene75760.t1 MTR_4g072300 49.180 61 30 1 13 72 26 86 8.77e-14 61.6
MS.gene75760.t1 MTR_4g072820 51.667 60 28 1 11 69 18 77 1.18e-13 61.2
MS.gene75760.t1 MTR_8g101530 45.714 70 38 0 11 80 38 107 1.33e-13 62.8
MS.gene75760.t1 MTR_3g084260 47.761 67 34 1 13 78 26 92 1.38e-13 61.2
MS.gene75760.t1 MTR_4g072510 50.820 61 29 1 13 72 21 81 1.42e-13 61.2
MS.gene75760.t1 MTR_1g061570 46.970 66 35 0 13 78 19 84 1.52e-13 62.0
MS.gene75760.t1 MTR_4g072630 50.000 62 30 1 13 73 21 82 1.55e-13 60.8
MS.gene75760.t1 MTR_3g110032 43.590 78 43 1 2 78 15 92 1.67e-13 61.2
MS.gene75760.t1 MTR_4g072490 43.662 71 39 1 3 72 10 80 1.90e-13 60.8
MS.gene75760.t1 MTR_7g104950 43.548 62 35 0 16 77 53 114 2.26e-13 61.6
MS.gene75760.t1 MTR_4g072540 50.820 61 29 1 13 72 21 81 2.56e-13 60.5
MS.gene75760.t1 MTR_4g072530 50.820 61 29 1 13 72 25 85 2.73e-13 60.5
MS.gene75760.t1 MTR_8g076040 42.188 64 37 0 13 76 41 104 3.51e-13 60.5
MS.gene75760.t1 MTR_3g117630 54.098 61 27 1 13 72 25 85 3.53e-13 60.1
MS.gene75760.t1 MTR_4g072450 50.000 62 30 1 12 72 19 80 4.03e-13 60.1
MS.gene75760.t1 MTR_4g072850 52.459 61 28 1 13 72 27 87 5.47e-13 59.7
MS.gene75760.t1 MTR_4g072590 50.000 58 28 1 13 69 20 77 6.57e-13 59.3
MS.gene75760.t1 MTR_4g072670 50.820 61 29 1 13 72 21 81 7.10e-13 59.3
MS.gene75760.t1 MTR_4g072560 43.038 79 43 2 1 77 20 98 8.61e-13 59.3
MS.gene75760.t1 MTR_4g072465 50.820 61 29 1 13 72 21 81 8.82e-13 58.9
MS.gene75760.t1 MTR_4g072830 43.590 78 33 2 13 89 23 90 9.51e-13 58.9
MS.gene75760.t1 MTR_4g072580 49.180 61 30 1 13 72 20 80 1.16e-12 58.9
MS.gene75760.t1 MTR_3g084200 59.091 44 18 0 13 56 25 68 1.51e-12 58.9
MS.gene75760.t1 MTR_4g072230 49.254 67 31 2 13 78 26 90 1.71e-12 58.5
MS.gene75760.t1 MTR_4g025830 42.105 76 40 2 9 80 75 150 2.13e-12 59.7
MS.gene75760.t1 MTR_4g072860 49.254 67 33 1 13 78 26 92 2.54e-12 58.2
MS.gene75760.t1 MTR_4g072600 45.714 70 37 1 4 72 11 80 3.66e-12 57.4
MS.gene75760.t1 MTR_5g008360 46.269 67 35 1 13 78 16 82 3.67e-12 57.4
MS.gene75760.t1 MTR_1g094750 50.820 61 30 0 13 73 50 110 4.95e-12 57.8
MS.gene75760.t1 MTR_3g110045 53.448 58 26 1 13 69 84 141 5.16e-12 58.5
MS.gene75760.t1 MTR_3g084210 46.269 67 35 1 13 78 25 91 5.77e-12 57.0
MS.gene75760.t1 MTR_5g008350 51.923 52 24 1 14 64 21 72 6.35e-12 56.6
MS.gene75760.t1 MTR_6g077850 40.580 69 40 1 10 77 31 99 6.73e-12 57.0
MS.gene75760.t1 MTR_0388s0020 36.905 84 50 1 4 84 66 149 7.36e-12 58.2
MS.gene75760.t1 MTR_4g072770 45.588 68 36 1 11 77 13 80 8.41e-12 56.2
MS.gene75760.t1 MTR_7g101920 42.105 76 42 1 1 74 23 98 8.55e-12 57.4
MS.gene75760.t1 MTR_8g461420 51.724 58 27 1 13 69 25 82 8.80e-12 56.6
MS.gene75760.t1 MTR_3g084190 50.000 58 28 1 13 69 25 82 1.88e-11 55.8
MS.gene75760.t1 MTR_4g072280 61.905 42 16 0 13 54 26 67 2.04e-11 55.5
MS.gene75760.t1 MTR_1g063310 40.741 81 43 2 10 88 23 100 2.34e-11 55.8
MS.gene75760.t1 MTR_4g072380 48.333 60 30 1 11 69 19 78 3.21e-11 55.1
MS.gene75760.t1 MTR_3g084230 50.000 58 28 1 13 69 25 82 4.80e-11 54.7
MS.gene75760.t1 MTR_3g117640 52.542 59 26 2 13 69 34 92 6.24e-11 54.7
MS.gene75760.t1 MTR_4g005310 40.000 70 40 1 6 73 55 124 6.49e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75760.t1 AT4G31320 64.835 91 27 2 12 97 79 169 5.31e-37 124
MS.gene75760.t1 AT2G24400 64.368 87 24 1 11 90 62 148 3.67e-35 118
MS.gene75760.t1 AT3G43120 54.167 72 33 0 13 84 79 150 2.21e-23 88.2
MS.gene75760.t1 AT5G20810 60.606 66 26 0 13 78 79 144 3.49e-23 87.8
MS.gene75760.t1 AT5G20810 61.538 65 25 0 13 77 79 143 4.06e-23 88.2
MS.gene75760.t1 AT1G56150 49.231 65 33 0 10 74 35 99 6.13e-17 70.5
MS.gene75760.t1 AT3G20220 51.515 66 31 1 9 73 43 108 1.78e-16 69.3
MS.gene75760.t1 AT3G61900 52.239 67 29 1 13 76 30 96 2.21e-16 69.7
MS.gene75760.t1 AT4G34750 47.297 74 35 1 7 76 27 100 5.07e-16 68.9
MS.gene75760.t1 AT4G34750 47.297 74 35 1 7 76 27 100 5.07e-16 68.9
MS.gene75760.t1 AT4G36110 45.205 73 40 0 3 75 30 102 7.38e-16 67.4
MS.gene75760.t1 AT4G34760 38.272 81 45 1 1 76 26 106 1.44e-15 66.6
MS.gene75760.t1 AT3G12830 43.662 71 40 0 4 74 41 111 1.64e-15 67.4
MS.gene75760.t1 AT2G21210 54.545 66 26 3 8 69 22 87 1.76e-15 66.2
MS.gene75760.t1 AT1G19840 51.562 64 31 0 13 76 41 104 2.92e-15 67.0
MS.gene75760.t1 AT2G18010 46.032 63 34 0 13 75 48 110 3.37e-15 65.9
MS.gene75760.t1 AT1G75580 45.946 74 39 1 3 76 35 107 3.72e-15 65.9
MS.gene75760.t1 AT2G21220 46.875 64 34 0 13 76 40 103 5.15e-15 65.5
MS.gene75760.t1 AT2G21210 48.148 81 32 4 8 84 89 163 6.40e-15 66.6
MS.gene75760.t1 AT5G10990 47.887 71 37 0 13 83 41 111 7.12e-15 66.2
MS.gene75760.t1 AT5G66260 43.284 67 38 0 11 77 32 98 8.18e-15 64.7
MS.gene75760.t1 AT4G38860 49.231 65 32 1 13 76 40 104 2.30e-14 63.5
MS.gene75760.t1 AT4G38840 55.932 59 24 2 13 69 33 91 2.50e-14 63.5
MS.gene75760.t1 AT2G46690 42.857 70 37 1 13 79 24 93 2.70e-14 63.9
MS.gene75760.t1 AT4G00880 47.887 71 35 1 9 77 24 94 5.58e-14 63.2
MS.gene75760.t1 AT1G19830 42.254 71 41 0 3 73 38 108 7.91e-14 62.8
MS.gene75760.t1 AT1G79130 45.312 64 35 0 11 74 46 109 1.23e-13 62.4
MS.gene75760.t1 AT3G60690 42.683 82 40 2 11 89 86 163 1.37e-13 63.2
MS.gene75760.t1 AT1G75590 50.000 64 32 0 13 76 42 105 1.49e-13 62.8
MS.gene75760.t1 AT4G34770 48.000 75 35 3 8 79 31 104 1.50e-13 61.6
MS.gene75760.t1 AT2G16580 36.585 82 46 1 1 76 26 107 1.56e-13 61.6
MS.gene75760.t1 AT1G16510 42.424 66 38 0 11 76 52 117 2.81e-13 62.0
MS.gene75760.t1 AT3G51200 46.154 65 34 1 13 76 39 103 9.14e-13 59.7
MS.gene75760.t1 AT4G12410 42.667 75 40 1 9 80 72 146 2.11e-12 59.7
MS.gene75760.t1 AT4G38825 40.000 70 40 2 5 72 15 84 2.91e-12 57.8
MS.gene75760.t1 AT4G34800 41.558 77 41 2 1 73 9 85 3.53e-12 57.8
MS.gene75760.t1 AT5G53590 41.429 70 36 1 13 77 46 115 4.23e-12 58.9
MS.gene75760.t1 AT5G18080 40.278 72 41 2 8 77 19 90 5.34e-12 57.4
MS.gene75760.t1 AT4G22620 43.056 72 38 1 12 80 78 149 6.99e-12 58.5
MS.gene75760.t1 AT4G13790 42.647 68 36 2 12 76 24 91 7.03e-12 57.0
MS.gene75760.t1 AT4G34780 44.156 77 39 2 13 85 28 104 1.84e-11 56.2
MS.gene75760.t1 AT5G18030 44.444 63 33 2 9 69 18 80 2.10e-11 55.5
MS.gene75760.t1 AT3G03850 43.836 73 38 2 9 78 21 93 2.27e-11 55.8
MS.gene75760.t1 AT2G36210 41.791 67 38 1 13 78 54 120 3.79e-11 56.2
MS.gene75760.t1 AT5G18010 43.750 64 34 2 8 69 19 82 4.13e-11 55.1
MS.gene75760.t1 AT2G45210 44.615 65 33 1 11 72 77 141 4.15e-11 56.6

Find 0 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTCTCTGAGTAATATATGAA+AGG - 24216:8405-8424 MS.gene75760:intergenic 25.0%
TCTACTCAGAAATGTAGATT+TGG + 24216:8530-8549 MS.gene75760:CDS 30.0%
TGTTATACCAACTGAGTATT+TGG + 24216:8370-8389 MS.gene75760:CDS 30.0%
TTGGTATAACAAACCTGTTA+AGG - 24216:8361-8380 MS.gene75760:intergenic 30.0%
! ATTTGGCATTGAAAAGATGA+TGG + 24216:8547-8566 MS.gene75760:CDS 30.0%
! TTTGGCATTGAAAAGATGAT+GGG + 24216:8548-8567 MS.gene75760:CDS 30.0%
AGAGAAGCTGAAGAAGAATT+TGG + 24216:8419-8438 MS.gene75760:CDS 35.0%
AGTATCTTGAAGATGGTTGA+AGG + 24216:8494-8513 MS.gene75760:CDS 35.0%
GAGAAGCTGAAGAAGAATTT+GGG + 24216:8420-8439 MS.gene75760:CDS 35.0%
TCAAACACTGAAACTTCACA+AGG - 24216:8473-8492 MS.gene75760:intergenic 35.0%
! GAATTTGGGTTTGAACAAAC+TGG + 24216:8434-8453 MS.gene75760:CDS 35.0%
!! GTTTGAGAGTATCTTGAAGA+TGG + 24216:8487-8506 MS.gene75760:CDS 35.0%
AAGGTCTACACCAACACAAA+CGG - 24216:8342-8361 MS.gene75759:intergenic 40.0%
CTTGAAGATGGTTGAAGGAA+AGG + 24216:8499-8518 MS.gene75760:CDS 40.0%
TGATGAGCCAAATACTCAGT+TGG - 24216:8380-8399 MS.gene75760:intergenic 40.0%
! GTGTTGGTGTAGACCTTAAC+AGG + 24216:8345-8364 MS.gene75760:CDS 45.0%
AGGAGGAGGATCAAACAACG+CGG + 24216:8298-8317 MS.gene75760:CDS 50.0%
CAAACGGCTAGGTAGCCTTT+CGG - 24216:8326-8345 MS.gene75760:intergenic 50.0%
CTACACCAACACAAACGGCT+AGG - 24216:8337-8356 MS.gene75760:intergenic 50.0%
TCAAACAACGCGGTTCCGAA+AGG + 24216:8308-8327 MS.gene75760:CDS 50.0%
GGCTACCTAGCCGTTTGTGT+TGG + 24216:8329-8348 MS.gene75760:CDS 55.0%
TGATCCTCCTCCTTCCCGTT+CGG - 24216:8291-8310 MS.gene75760:intergenic 55.0%
ATGTCCGAACGGGAAGGAGG+AGG + 24216:8284-8303 MS.gene75760:CDS 60.0%


Chromosome Type Strat End Strand Name
24216 gene 8284 8593 8284 ID=MS.gene75760
24216 mRNA 8284 8593 8284 ID=MS.gene75760.t1;Parent=MS.gene75760
24216 exon 8284 8593 8284 ID=MS.gene75760.t1.exon1;Parent=MS.gene75760.t1
24216 CDS 8284 8593 8284 ID=cds.MS.gene75760.t1;Parent=MS.gene75760.t1
Gene Sequence

>MS.gene75760

ATGTCCGAACGGGAAGGAGGAGGATCAAACAACGCGGTTCCGAAAGGCTACCTAGCCGTTTGTGTTGGTGTAGACCTTAACAGGTTTGTTATACCAACTGAGTATTTGGCTCATCAAGCCTTTCATATATTACTCAGAGAAGCTGAAGAAGAATTTGGGTTTGAACAAACTGGTGTTCTGAGAATTCCTTGTGAAGTTTCAGTGTTTGAGAGTATCTTGAAGATGGTTGAAGGAAAGGATAGATTTTCTACTCAGAAATGTAGATTTGGCATTGAAAAGATGATGGGATACTGTTCCTCAAACCAACTTG
Protein sequence

>MS.gene75760.t1

MSEREGGGSNNAVPKGYLAVCVGVDLNRFVIPTEYLAHQAFHILLREAEEEFGFEQTGVLRIPCEVSVFESILKMVEGKDRFSTQKCRFGIEKMMGYCSSNQL