Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76701.t1 | RHN59397.1 | 95 | 119 | 6 | 0 | 1 | 119 | 22 | 140 | 2.10E-54 | 221.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76701.t1 | G7JIA9 | 95.0 | 119 | 6 | 0 | 1 | 119 | 1 | 119 | 1.5e-54 | 221.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049850 | MS.gene76701 | 0.803226 | 3.86E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene76701 | 0.802581 | 5.24E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene76701 | 0.811135 | 8.15E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene76701 | 0.804795 | 1.82E-49 | -1.69E-46 |
| MS.gene05157 | MS.gene76701 | 0.820344 | 7.21E-53 | -1.69E-46 |
| MS.gene051719 | MS.gene76701 | 0.834427 | 3.02E-56 | -1.69E-46 |
| MS.gene051720 | MS.gene76701 | 0.850107 | 2.10E-60 | -1.69E-46 |
| MS.gene054775 | MS.gene76701 | 0.808895 | 2.47E-50 | -1.69E-46 |
| MS.gene054801 | MS.gene76701 | 0.816196 | 6.27E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene76701 | 0.823817 | 1.13E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene76701 | 0.83749 | 5.04E-57 | -1.69E-46 |
| MS.gene055468 | MS.gene76701 | 0.812102 | 5.02E-51 | -1.69E-46 |
| MS.gene058339 | MS.gene76701 | 0.805423 | 1.34E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76701.t1 | MTR_4g028110 | 94.958 | 119 | 6 | 0 | 1 | 119 | 1 | 119 | 1.98e-78 | 226 |
| MS.gene76701.t1 | MTR_7g073270 | 49.593 | 123 | 52 | 5 | 4 | 119 | 1 | 120 | 1.09e-23 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCAGAACCAAGATTTCTA+AGG | 0.315908 | 4.1:-71371959 | MS.gene76701:CDS |
| GACACTGACCTTAGAAATCT+TGG | 0.321072 | 4.1:+71371951 | None:intergenic |
| GCAATGATTCGAAAGAGATT+TGG | 0.327865 | 4.1:-71372065 | MS.gene76701:CDS |
| TCAGAATCCACTCTCTTTCA+AGG | 0.337545 | 4.1:-71371766 | MS.gene76701:CDS |
| GGAAGACGTTGTCGCGGTTC+CGG | 0.343136 | 4.1:-71371867 | MS.gene76701:CDS |
| CTTAAACACCTTTCGAAAAC+CGG | 0.347709 | 4.1:+71371848 | None:intergenic |
| AAATTGTTCAAACCATTTCT+TGG | 0.349128 | 4.1:-71371825 | MS.gene76701:CDS |
| CAAAGGATAGCCTCTCAAAT+TGG | 0.423678 | 4.1:+71372018 | None:intergenic |
| TGAGTGACAAGAGGAACTGC+TGG | 0.485543 | 4.1:+71371903 | None:intergenic |
| AGCAGTTCCTCTTGTCACTC+AGG | 0.488848 | 4.1:-71371901 | MS.gene76701:CDS |
| TTTGTTGAGTTGAGACTCAA+AGG | 0.523465 | 4.1:+71372001 | None:intergenic |
| TGATAAGCTTCTTGATAACA+TGG | 0.525013 | 4.1:+71371977 | None:intergenic |
| CTAAATGCCTTGAAAGAGAG+TGG | 0.545008 | 4.1:+71371759 | None:intergenic |
| GTCACTCAGGAGGCGAAGCA+AGG | 0.560619 | 4.1:-71371888 | MS.gene76701:CDS |
| AGGCATTTAGTAGATCATTG+AGG | 0.589944 | 4.1:-71371746 | MS.gene76701:CDS |
| TTCGCCTCCTGAGTGACAAG+AGG | 0.607306 | 4.1:+71371894 | None:intergenic |
| ATTTGTTAGACCAATTTGAG+AGG | 0.623039 | 4.1:-71372028 | MS.gene76701:CDS |
| AGTTCCTCTTGTCACTCAGG+AGG | 0.660481 | 4.1:-71371898 | MS.gene76701:CDS |
| AAGCAAGGAAGACGTTGTCG+CGG | 0.700615 | 4.1:-71371873 | MS.gene76701:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGTTCAAACCATTTCT+TGG | - | chr4.1:71371992-71372011 | MS.gene76701:CDS | 25.0% |
| CCATTTCTTGGTAGAAAAAA+TGG | - | chr4.1:71372004-71372023 | MS.gene76701:CDS | 30.0% | |
| TATCAGAACCAAGATTTCTA+AGG | - | chr4.1:71371858-71371877 | MS.gene76701:CDS | 30.0% | |
| ! | ATTTGTTAGACCAATTTGAG+AGG | - | chr4.1:71371789-71371808 | MS.gene76701:CDS | 30.0% |
| ! | TGATAAGCTTCTTGATAACA+TGG | + | chr4.1:71371843-71371862 | None:intergenic | 30.0% |
| !!! | CCATTTTTTCTACCAAGAAA+TGG | + | chr4.1:71372007-71372026 | None:intergenic | 30.0% |
| AGGCATTTAGTAGATCATTG+AGG | - | chr4.1:71372071-71372090 | MS.gene76701:CDS | 35.0% | |
| CTTAAACACCTTTCGAAAAC+CGG | + | chr4.1:71371972-71371991 | None:intergenic | 35.0% | |
| GCAATGATTCGAAAGAGATT+TGG | - | chr4.1:71371752-71371771 | MS.gene76701:CDS | 35.0% | |
| TTGGTAGAAAAAATGGAGCA+AGG | - | chr4.1:71372011-71372030 | MS.gene76701:CDS | 35.0% | |
| !! | TTTGTTGAGTTGAGACTCAA+AGG | + | chr4.1:71371819-71371838 | None:intergenic | 35.0% |
| CAAAGGATAGCCTCTCAAAT+TGG | + | chr4.1:71371802-71371821 | None:intergenic | 40.0% | |
| CTAAATGCCTTGAAAGAGAG+TGG | + | chr4.1:71372061-71372080 | None:intergenic | 40.0% | |
| GACACTGACCTTAGAAATCT+TGG | + | chr4.1:71371869-71371888 | None:intergenic | 40.0% | |
| TCAGAATCCACTCTCTTTCA+AGG | - | chr4.1:71372051-71372070 | MS.gene76701:CDS | 40.0% | |
| AAGCAAGGAAGACGTTGTCG+CGG | - | chr4.1:71371944-71371963 | MS.gene76701:CDS | 50.0% | |
| AGCAGTTCCTCTTGTCACTC+AGG | - | chr4.1:71371916-71371935 | MS.gene76701:CDS | 50.0% | |
| AGTTCCTCTTGTCACTCAGG+AGG | - | chr4.1:71371919-71371938 | MS.gene76701:CDS | 50.0% | |
| TGAGTGACAAGAGGAACTGC+TGG | + | chr4.1:71371917-71371936 | None:intergenic | 50.0% | |
| TTCGCCTCCTGAGTGACAAG+AGG | + | chr4.1:71371926-71371945 | None:intergenic | 55.0% | |
| ! | TCGCGGTTCCGGTTTTCGAA+AGG | - | chr4.1:71371961-71371980 | MS.gene76701:CDS | 55.0% |
| GGAAGACGTTGTCGCGGTTC+CGG | - | chr4.1:71371950-71371969 | MS.gene76701:CDS | 60.0% | |
| GTCACTCAGGAGGCGAAGCA+AGG | - | chr4.1:71371929-71371948 | MS.gene76701:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 71371740 | 71372099 | 71371740 | ID=MS.gene76701 |
| chr4.1 | mRNA | 71371740 | 71372099 | 71371740 | ID=MS.gene76701.t1;Parent=MS.gene76701 |
| chr4.1 | exon | 71371740 | 71372099 | 71371740 | ID=MS.gene76701.t1.exon1;Parent=MS.gene76701.t1 |
| chr4.1 | CDS | 71371740 | 71372099 | 71371740 | ID=cds.MS.gene76701.t1;Parent=MS.gene76701.t1 |
| Gene Sequence |
| Protein sequence |
