AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76794

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76794.t1 MTR_2g046710 99.495 396 2 0 1 396 1 396 0.0 816
MS.gene76794.t1 MTR_4g123810 93.622 392 25 0 1 392 1 392 0.0 741
MS.gene76794.t1 MTR_7g110310 93.316 389 26 0 4 392 3 391 0.0 739
MS.gene76794.t1 MTR_7g102120 90.415 386 37 0 4 389 2 387 0.0 704
MS.gene76794.t1 MTR_1g063060 87.824 386 47 0 4 389 2 387 0.0 693
MS.gene76794.t1 MTR_1g063060 87.824 386 47 0 4 389 20 405 0.0 692
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76794.t1 AT3G17390 94.103 390 23 0 4 393 2 391 0.0 743
MS.gene76794.t1 AT1G02500 92.545 389 29 0 4 392 2 390 0.0 724
MS.gene76794.t1 AT1G02500 92.545 389 29 0 4 392 2 390 0.0 724
MS.gene76794.t1 AT4G01850 91.003 389 35 0 4 392 2 390 0.0 721
MS.gene76794.t1 AT4G01850 91.003 389 35 0 4 392 2 390 0.0 721
MS.gene76794.t1 AT2G36880 90.415 386 37 0 4 389 2 387 0.0 707
MS.gene76794.t1 AT2G36880 90.415 386 37 0 4 389 2 387 0.0 707

Find 111 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGCTGCTTATGGACATTT+TGG 0.240476 2.4:+44176194 MS.gene76794:CDS
ATGTTAAGGATTTCCTTATC+AGG 0.261463 2.4:-44176086 None:intergenic
TTCCTTATCAGGGATCTTTC+CGG 0.289318 2.4:-44176075 None:intergenic
GTTGCAAGAACATGGGTTAA+AGG 0.294303 2.4:-44175516 None:intergenic
CATGTTCTTGCAACCAAGTT+AGG 0.294943 2.4:+44175525 MS.gene76794:CDS
ACCAACATGGTTATGGTCTT+TGG 0.296902 2.4:+44175243 MS.gene76794:CDS
TTCTCATCAAGGTACTTCTC+TGG 0.310083 2.4:-44175759 None:intergenic
CATCGATACCTACGGTGGTT+GGG 0.340499 2.4:+44175866 MS.gene76794:CDS
ATCTGGTCGCAAAGCTTGTC+GGG 0.354536 2.4:-44175147 None:intergenic
TGAAAGAAACCTTTGACTTC+AGG 0.356558 2.4:+44176111 MS.gene76794:CDS
TTAAGAGGGGTGGAAATAAC+AGG 0.368924 2.4:+44176162 MS.gene76794:CDS
TCACATGCAACTTTGCTGTC+TGG 0.378990 2.4:-44175207 None:intergenic
ATCAGCTGCAATTTCATCAT+TGG 0.382947 2.4:-44175706 None:intergenic
GTGATCAAGGTCACATGTTC+GGG 0.391846 2.4:+44175463 MS.gene76794:CDS
CTATCAACCTTGATCTTAAG+AGG 0.391961 2.4:+44176147 MS.gene76794:CDS
GATATTGCTCAAGGTGTTCA+TGG 0.399050 2.4:+44175405 MS.gene76794:CDS
GGTGATCAAGGTCACATGTT+CGG 0.414273 2.4:+44175462 MS.gene76794:CDS
CACAAAGACCAACATGGTTA+TGG 0.418184 2.4:+44175236 MS.gene76794:CDS
AGGTTTCTTTCACAATGTTA+AGG 0.420122 2.4:-44176100 None:intergenic
TTCCACCCCTCTTAAGATCA+AGG 0.427046 2.4:-44176154 None:intergenic
TCTCATCAAGGTACTTCTCT+GGG 0.433415 2.4:-44175758 None:intergenic
AGGTTGATAGAGATCATTCC+TGG 0.433466 2.4:-44176134 None:intergenic
CACGGTGACGCTGGTCTAAC+CGG 0.435684 2.4:+44175831 MS.gene76794:CDS
ATCTTCCACTTGAACCCTTC+AGG 0.443169 2.4:+44175786 MS.gene76794:CDS
TGAACACCTTGAGCAATATC+AGG 0.445358 2.4:-44175402 None:intergenic
TCACAGATTCAGATGTGTAT+AGG 0.445528 2.4:-44175113 None:intergenic
GGTTGGGGTGCACACGGTGG+TGG 0.446281 2.4:+44175882 MS.gene76794:CDS
CACGGTGGTGGTGCTTTCTC+CGG 0.452162 2.4:+44175894 MS.gene76794:CDS
GCAAAGAGTATTGTTGCTAA+TGG 0.456252 2.4:+44175969 MS.gene76794:CDS
CTAACTTGGTTGCAAGAACA+TGG 0.459951 2.4:-44175524 None:intergenic
CGTCGTGCTGTGTTGAGATA+AGG 0.460565 2.4:-44175674 None:intergenic
CAAGAACGGCATCAGAGATC+TGG 0.466047 2.4:-44175164 None:intergenic
GAAACCTTTGACTTCAGGCC+AGG 0.467102 2.4:+44176116 MS.gene76794:CDS
GTGACTTGGGTCTTTCCATC+AGG 0.467493 2.4:-44175597 None:intergenic
CCTTCAGGCCGTTTCGTCAT+CGG 0.467571 2.4:+44175801 MS.gene76794:CDS
TTGCTAATGGACTTGCTAGA+AGG 0.469452 2.4:+44175982 MS.gene76794:CDS
ATCGATACCTACGGTGGTTG+GGG 0.475144 2.4:+44175867 MS.gene76794:CDS
ACAAACACGGACAATGGCTC+TGG 0.479016 2.4:-44176038 None:intergenic
CGTAACACATGCAGGAACAT+AGG 0.489461 2.4:+44175309 MS.gene76794:CDS
TTTGTTGATTCTTACGGTAC+CGG 0.493288 2.4:+44176056 MS.gene76794:CDS
TGAAGGGTTCAAGTGGAAGA+TGG 0.500503 2.4:-44175784 None:intergenic
TCCGTGTTTGTTGATTCTTA+CGG 0.503083 2.4:+44176050 MS.gene76794:CDS
GATCTGGTCGCAAAGCTTGT+CGG 0.504899 2.4:-44175148 None:intergenic
AGTGAGCTTAGCTCCTAACT+TGG 0.505858 2.4:-44175538 None:intergenic
GAAATTGGTGCTGGTGATCA+AGG 0.514486 2.4:+44175450 MS.gene76794:CDS
TGTTAAGGATTTCCTTATCA+GGG 0.514572 2.4:-44176085 None:intergenic
TACGGTGGTTGGGGTGCACA+CGG 0.522520 2.4:+44175876 MS.gene76794:CDS
AGGTACTTCTCTGGGATCAC+AGG 0.524549 2.4:-44175750 None:intergenic
GTGCAAGTTTCCTATGCTAT+CGG 0.528063 2.4:+44176011 MS.gene76794:CDS
TGCTGTCTGGATCTTGCTCA+AGG 0.528629 2.4:-44175194 None:intergenic
GCAAGAACGGAACATGCCCT+TGG 0.529608 2.4:+44175568 MS.gene76794:CDS
TACCGGAAAGATCCCTGATA+AGG 0.532446 2.4:+44176073 MS.gene76794:CDS
GCTAACTTTCCACCCTTGAG+TGG 0.533954 2.4:-44176254 None:intergenic
TCATCGATACCTACGGTGGT+TGG 0.534200 2.4:+44175865 MS.gene76794:CDS
TTCGTCATCGGTGGTCCTCA+CGG 0.537678 2.4:+44175813 MS.gene76794:CDS
TCACCAGCACCAATTTCCTC+AGG 0.537693 2.4:-44175444 None:intergenic
GGTGGTCCTCACGGTGACGC+TGG 0.538563 2.4:+44175822 MS.gene76794:CDS
CATTCCTGGCCTGAAGTCAA+AGG 0.538937 2.4:-44176120 None:intergenic
CTCACTGAAGTTCGCAAGAA+CGG 0.541655 2.4:+44175555 MS.gene76794:CDS
AACCTTGATCTTAAGAGGGG+TGG 0.542096 2.4:+44176152 MS.gene76794:CDS
CGATGACGAAACGGCCTGAA+GGG 0.544735 2.4:-44175800 None:intergenic
GAAATACTCAATAGTGACTT+GGG 0.547189 2.4:-44175610 None:intergenic
CATAAGCTCAGGTGTTTCAT+CGG 0.547507 2.4:-44175493 None:intergenic
TGAAATACTCAATAGTGACT+TGG 0.552787 2.4:-44175611 None:intergenic
TGCCCTTGGTTGAGACCTGA+TGG 0.559477 2.4:+44175582 MS.gene76794:CDS
GAGGACCACCGATGACGAAA+CGG 0.563585 2.4:-44175809 None:intergenic
AAGATCCAACAAAAGTCGAC+AGG 0.563704 2.4:+44175919 MS.gene76794:CDS
TGGGTTAAAGGCATAAGCTC+AGG 0.565243 2.4:-44175504 None:intergenic
TTTCAATGACAAGGGTGCCA+TGG 0.566099 2.4:+44175629 MS.gene76794:CDS
CACATCTGAATCTGTGAACG+AGG 0.567405 2.4:+44175119 MS.gene76794:CDS
GGAAGTTGTGAAGCCACTCA+AGG 0.570115 2.4:+44176241 MS.gene76794:CDS
TATCAACCTTGATCTTAAGA+GGG 0.570559 2.4:+44176148 MS.gene76794:CDS
CCGATGACGAAACGGCCTGA+AGG 0.572989 2.4:-44175801 None:intergenic
AGATTGTTCGTAACACATGC+AGG 0.575918 2.4:+44175301 MS.gene76794:CDS
TTCCATCAGGTCTCAACCAA+GGG 0.579295 2.4:-44175584 None:intergenic
ATCGATGATGATCTTGCGGC+CGG 0.584426 2.4:-44175850 None:intergenic
GTGTTGAGATAAGGACGGTA+TGG 0.586897 2.4:-44175665 None:intergenic
CCATCACTTCACTTAGGCAG+TGG 0.590313 2.4:-44176276 None:intergenic
TATTGAGTATTTCAATGACA+AGG 0.592614 2.4:+44175620 MS.gene76794:CDS
AGACCTGAGGAAATTGGTGC+TGG 0.593942 2.4:+44175441 MS.gene76794:CDS
GAATCAACAAACACGGACAA+TGG 0.604860 2.4:-44176044 None:intergenic
TCAGGCCGTTTCGTCATCGG+TGG 0.606053 2.4:+44175804 MS.gene76794:CDS
AACGGCCTGAAGGGTTCAAG+TGG 0.607726 2.4:-44175791 None:intergenic
CAGAGTCCTGATATTGCTCA+AGG 0.609518 2.4:+44175396 MS.gene76794:CDS
GAGATGATGCTGATTTCACA+TGG 0.613131 2.4:+44176219 MS.gene76794:CDS
CTCAAGGCAAGCATCAAGAA+CGG 0.613435 2.4:-44175178 None:intergenic
ATTGAGTATTTCAATGACAA+GGG 0.615601 2.4:+44175621 MS.gene76794:CDS
ACCAAAGACCATAACCATGT+TGG 0.617396 2.4:-44175244 None:intergenic
CTCTGGAACACCGATAGCAT+AGG 0.620591 2.4:-44176021 None:intergenic
AGGACGGTATGGACACGGAT+CGG 0.620832 2.4:-44175654 None:intergenic
AGATAAGGACGGTATGGACA+CGG 0.625832 2.4:-44175659 None:intergenic
TTTCCATCAGGTCTCAACCA+AGG 0.628406 2.4:-44175585 None:intergenic
CCAACAAAAGTCGACAGGAG+TGG 0.630861 2.4:+44175924 MS.gene76794:CDS
GAAGTTGTGAAGCCACTCAA+GGG 0.631025 2.4:+44176242 MS.gene76794:CDS
AAGATCATCATCGATACCTA+CGG 0.632923 2.4:+44175858 MS.gene76794:CDS
TCTGTTGTTCAATGTTGACA+AGG 0.633149 2.4:-44175377 None:intergenic
TAACTTGGTTGCAAGAACAT+GGG 0.635065 2.4:-44175523 None:intergenic
ATGGACACGGATCGGAACCA+TGG 0.635111 2.4:-44175646 None:intergenic
AGGTATCGATGATGATCTTG+CGG 0.652737 2.4:-44175854 None:intergenic
GTGTGCACCCCAACCACCGT+AGG 0.659434 2.4:-44175874 None:intergenic
ATCAACCTTGATCTTAAGAG+GGG 0.662473 2.4:+44176149 MS.gene76794:CDS
GTTGTGAAGCCACTCAAGGG+TGG 0.662760 2.4:+44176245 MS.gene76794:CDS
GGTGGTTGGGGTGCACACGG+TGG 0.663588 2.4:+44175879 MS.gene76794:CDS
TCTGGTCGCAAAGCTTGTCG+GGG 0.671297 2.4:-44175146 None:intergenic
ACCGTAAGAATCAACAAACA+CGG 0.692869 2.4:-44176051 None:intergenic
ACATCTGAATCTGTGAACGA+GGG 0.708400 2.4:+44175120 MS.gene76794:CDS
AGATGATGCTGATTTCACAT+GGG 0.714850 2.4:+44176220 MS.gene76794:CDS
GTTAGACCAGCGTCACCGTG+AGG 0.718676 2.4:-44175828 None:intergenic
ATCATCATCGATACCTACGG+TGG 0.735632 2.4:+44175861 MS.gene76794:CDS
GTGCTGTGTTGAGATAAGGA+CGG 0.743744 2.4:-44175670 None:intergenic
AACTTGCACAAAGACCAACA+TGG 0.753910 2.4:+44175230 MS.gene76794:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTGAGTATTTCAATGACAA+GGG + chr2.4:44175621-44175640 MS.gene76794:CDS 25.0%
! TATTGAGTATTTCAATGACA+AGG + chr2.4:44175620-44175639 MS.gene76794:CDS 25.0%
AGGTTTCTTTCACAATGTTA+AGG - chr2.4:44176103-44176122 None:intergenic 30.0%
ATGTTAAGGATTTCCTTATC+AGG - chr2.4:44176089-44176108 None:intergenic 30.0%
GAAATACTCAATAGTGACTT+GGG - chr2.4:44175613-44175632 None:intergenic 30.0%
TATCAACCTTGATCTTAAGA+GGG + chr2.4:44176148-44176167 MS.gene76794:CDS 30.0%
TGAAATACTCAATAGTGACT+TGG - chr2.4:44175614-44175633 None:intergenic 30.0%
TGTTAAGGATTTCCTTATCA+GGG - chr2.4:44176088-44176107 None:intergenic 30.0%
AAGATCATCATCGATACCTA+CGG + chr2.4:44175858-44175877 MS.gene76794:CDS 35.0%
ACAAAAAGACCTGAGGAAAT+TGG + chr2.4:44175435-44175454 MS.gene76794:CDS 35.0%
ACCGTAAGAATCAACAAACA+CGG - chr2.4:44176054-44176073 None:intergenic 35.0%
ATCAACCTTGATCTTAAGAG+GGG + chr2.4:44176149-44176168 MS.gene76794:CDS 35.0%
ATCAGCTGCAATTTCATCAT+TGG - chr2.4:44175709-44175728 None:intergenic 35.0%
CTATCAACCTTGATCTTAAG+AGG + chr2.4:44176147-44176166 MS.gene76794:CDS 35.0%
TAACTTGGTTGCAAGAACAT+GGG - chr2.4:44175526-44175545 None:intergenic 35.0%
TCACAGATTCAGATGTGTAT+AGG - chr2.4:44175116-44175135 None:intergenic 35.0%
TCTGTTGTTCAATGTTGACA+AGG - chr2.4:44175380-44175399 None:intergenic 35.0%
! GACTTTTGTTGGATCTTTAC+CGG - chr2.4:44175916-44175935 None:intergenic 35.0%
! TGAAAGAAACCTTTGACTTC+AGG + chr2.4:44176111-44176130 MS.gene76794:CDS 35.0%
!! AGATGATGCTGATTTCACAT+GGG + chr2.4:44176220-44176239 MS.gene76794:CDS 35.0%
!! GCAAAGAGTATTGTTGCTAA+TGG + chr2.4:44175969-44175988 MS.gene76794:CDS 35.0%
!! TCCGTGTTTGTTGATTCTTA+CGG + chr2.4:44176050-44176069 MS.gene76794:CDS 35.0%
!! TTTGTTGATTCTTACGGTAC+CGG + chr2.4:44176056-44176075 MS.gene76794:CDS 35.0%
!! TTTTTGAAGACTGCTGCTTA+TGG + chr2.4:44176185-44176204 MS.gene76794:CDS 35.0%
!!! GGTTTTGTTTCAGATGATGT+TGG + chr2.4:44175330-44175349 MS.gene76794:CDS 35.0%
AACTTGCACAAAGACCAACA+TGG + chr2.4:44175230-44175249 MS.gene76794:CDS 40.0%
AAGATCCAACAAAAGTCGAC+AGG + chr2.4:44175919-44175938 MS.gene76794:CDS 40.0%
ACATCTGAATCTGTGAACGA+GGG + chr2.4:44175120-44175139 MS.gene76794:CDS 40.0%
ACCAAAGACCATAACCATGT+TGG - chr2.4:44175247-44175266 None:intergenic 40.0%
ACCAACATGGTTATGGTCTT+TGG + chr2.4:44175243-44175262 MS.gene76794:CDS 40.0%
AGATTGTTCGTAACACATGC+AGG + chr2.4:44175301-44175320 MS.gene76794:CDS 40.0%
CACAAAGACCAACATGGTTA+TGG + chr2.4:44175236-44175255 MS.gene76794:CDS 40.0%
CATAAGCTCAGGTGTTTCAT+CGG - chr2.4:44175496-44175515 None:intergenic 40.0%
CATGTTCTTGCAACCAAGTT+AGG + chr2.4:44175525-44175544 MS.gene76794:CDS 40.0%
CCATCTTACAAAAAGACCTG+AGG + chr2.4:44175428-44175447 MS.gene76794:CDS 40.0%
CTAACTTGGTTGCAAGAACA+TGG - chr2.4:44175527-44175546 None:intergenic 40.0%
GAATCAACAAACACGGACAA+TGG - chr2.4:44176047-44176066 None:intergenic 40.0%
GTGCAAGTTTCCTATGCTAT+CGG + chr2.4:44176011-44176030 MS.gene76794:CDS 40.0%
TGAACACCTTGAGCAATATC+AGG - chr2.4:44175405-44175424 None:intergenic 40.0%
TTAAGAGGGGTGGAAATAAC+AGG + chr2.4:44176162-44176181 MS.gene76794:CDS 40.0%
TTCCTTATCAGGGATCTTTC+CGG - chr2.4:44176078-44176097 None:intergenic 40.0%
! CCTCAGGTCTTTTTGTAAGA+TGG - chr2.4:44175431-44175450 None:intergenic 40.0%
! GATATTGCTCAAGGTGTTCA+TGG + chr2.4:44175405-44175424 MS.gene76794:CDS 40.0%
! GGAAGATGGTTTTCTCATCA+AGG - chr2.4:44175773-44175792 None:intergenic 40.0%
! GTTGCAAGAACATGGGTTAA+AGG - chr2.4:44175519-44175538 None:intergenic 40.0%
! TCTCATCAAGGTACTTCTCT+GGG - chr2.4:44175761-44175780 None:intergenic 40.0%
! TTCTCATCAAGGTACTTCTC+TGG - chr2.4:44175762-44175781 None:intergenic 40.0%
!! ACTGCTGCTTATGGACATTT+TGG + chr2.4:44176194-44176213 MS.gene76794:CDS 40.0%
!! AGGTATCGATGATGATCTTG+CGG - chr2.4:44175857-44175876 None:intergenic 40.0%
!! AGGTTGATAGAGATCATTCC+TGG - chr2.4:44176137-44176156 None:intergenic 40.0%
!! GAGATGATGCTGATTTCACA+TGG + chr2.4:44176219-44176238 MS.gene76794:CDS 40.0%
!! TTGCTAATGGACTTGCTAGA+AGG + chr2.4:44175982-44176001 MS.gene76794:CDS 40.0%
AACCTTGATCTTAAGAGGGG+TGG + chr2.4:44176152-44176171 MS.gene76794:CDS 45.0%
AGATAAGGACGGTATGGACA+CGG - chr2.4:44175662-44175681 None:intergenic 45.0%
AGTGAGCTTAGCTCCTAACT+TGG - chr2.4:44175541-44175560 None:intergenic 45.0%
ATCATCATCGATACCTACGG+TGG + chr2.4:44175861-44175880 MS.gene76794:CDS 45.0%
ATCTTCCACTTGAACCCTTC+AGG + chr2.4:44175786-44175805 MS.gene76794:CDS 45.0%
CACATCTGAATCTGTGAACG+AGG + chr2.4:44175119-44175138 MS.gene76794:CDS 45.0%
CAGAGTCCTGATATTGCTCA+AGG + chr2.4:44175396-44175415 MS.gene76794:CDS 45.0%
CGTAACACATGCAGGAACAT+AGG + chr2.4:44175309-44175328 MS.gene76794:CDS 45.0%
CTCAAGGCAAGCATCAAGAA+CGG - chr2.4:44175181-44175200 None:intergenic 45.0%
CTCACTGAAGTTCGCAAGAA+CGG + chr2.4:44175555-44175574 MS.gene76794:CDS 45.0%
GAAGTTGTGAAGCCACTCAA+GGG + chr2.4:44176242-44176261 MS.gene76794:CDS 45.0%
GGTGATCAAGGTCACATGTT+CGG + chr2.4:44175462-44175481 MS.gene76794:CDS 45.0%
GTGATCAAGGTCACATGTTC+GGG + chr2.4:44175463-44175482 MS.gene76794:CDS 45.0%
GTGCTGTGTTGAGATAAGGA+CGG - chr2.4:44175673-44175692 None:intergenic 45.0%
GTGTTGAGATAAGGACGGTA+TGG - chr2.4:44175668-44175687 None:intergenic 45.0%
TACCGGAAAGATCCCTGATA+AGG + chr2.4:44176073-44176092 MS.gene76794:CDS 45.0%
TGAAGGGTTCAAGTGGAAGA+TGG - chr2.4:44175787-44175806 None:intergenic 45.0%
TTCCACCCCTCTTAAGATCA+AGG - chr2.4:44176157-44176176 None:intergenic 45.0%
TTCCATCAGGTCTCAACCAA+GGG - chr2.4:44175587-44175606 None:intergenic 45.0%
TTTCCATCAGGTCTCAACCA+AGG - chr2.4:44175588-44175607 None:intergenic 45.0%
! TCACATGCAACTTTGCTGTC+TGG - chr2.4:44175210-44175229 None:intergenic 45.0%
! TGGGTTAAAGGCATAAGCTC+AGG - chr2.4:44175507-44175526 None:intergenic 45.0%
!! GAAATTGGTGCTGGTGATCA+AGG + chr2.4:44175450-44175469 MS.gene76794:CDS 45.0%
!! TTTCAATGACAAGGGTGCCA+TGG + chr2.4:44175629-44175648 MS.gene76794:CDS 45.0%
ACAAACACGGACAATGGCTC+TGG - chr2.4:44176041-44176060 None:intergenic 50.0%
AGACCTGAGGAAATTGGTGC+TGG + chr2.4:44175441-44175460 MS.gene76794:CDS 50.0%
ATCGATACCTACGGTGGTTG+GGG + chr2.4:44175867-44175886 MS.gene76794:CDS 50.0%
ATCGATGATGATCTTGCGGC+CGG - chr2.4:44175853-44175872 None:intergenic 50.0%
ATCTGGTCGCAAAGCTTGTC+GGG - chr2.4:44175150-44175169 None:intergenic 50.0%
CAAGAACGGCATCAGAGATC+TGG - chr2.4:44175167-44175186 None:intergenic 50.0%
CATCGATACCTACGGTGGTT+GGG + chr2.4:44175866-44175885 MS.gene76794:CDS 50.0%
CATTCCTGGCCTGAAGTCAA+AGG - chr2.4:44176123-44176142 None:intergenic 50.0%
CCAACAAAAGTCGACAGGAG+TGG + chr2.4:44175924-44175943 MS.gene76794:CDS 50.0%
CGTCGTGCTGTGTTGAGATA+AGG - chr2.4:44175677-44175696 None:intergenic 50.0%
GATCTGGTCGCAAAGCTTGT+CGG - chr2.4:44175151-44175170 None:intergenic 50.0%
GCTAACTTTCCACCCTTGAG+TGG - chr2.4:44176257-44176276 None:intergenic 50.0%
GGAAGTTGTGAAGCCACTCA+AGG + chr2.4:44176241-44176260 MS.gene76794:CDS 50.0%
TCACCAGCACCAATTTCCTC+AGG - chr2.4:44175447-44175466 None:intergenic 50.0%
TCATCGATACCTACGGTGGT+TGG + chr2.4:44175865-44175884 MS.gene76794:CDS 50.0%
TGCTGTCTGGATCTTGCTCA+AGG - chr2.4:44175197-44175216 None:intergenic 50.0%
! GAAACCTTTGACTTCAGGCC+AGG + chr2.4:44176116-44176135 MS.gene76794:CDS 50.0%
! GTGACTTGGGTCTTTCCATC+AGG - chr2.4:44175600-44175619 None:intergenic 50.0%
!! AGGTACTTCTCTGGGATCAC+AGG - chr2.4:44175753-44175772 None:intergenic 50.0%
!! CTCTGGAACACCGATAGCAT+AGG - chr2.4:44176024-44176043 None:intergenic 50.0%
!!! CCACTCCTGTCGACTTTTGT+TGG - chr2.4:44175927-44175946 None:intergenic 50.0%
AACGGCCTGAAGGGTTCAAG+TGG - chr2.4:44175794-44175813 None:intergenic 55.0%
AGGACGGTATGGACACGGAT+CGG - chr2.4:44175657-44175676 None:intergenic 55.0%
ATGGACACGGATCGGAACCA+TGG - chr2.4:44175649-44175668 None:intergenic 55.0%
CCTTCAGGCCGTTTCGTCAT+CGG + chr2.4:44175801-44175820 MS.gene76794:CDS 55.0%
CGATGACGAAACGGCCTGAA+GGG - chr2.4:44175803-44175822 None:intergenic 55.0%
GAGGACCACCGATGACGAAA+CGG - chr2.4:44175812-44175831 None:intergenic 55.0%
GCAAGAACGGAACATGCCCT+TGG + chr2.4:44175568-44175587 MS.gene76794:CDS 55.0%
GTTGTGAAGCCACTCAAGGG+TGG + chr2.4:44176245-44176264 MS.gene76794:CDS 55.0%
TCTGGTCGCAAAGCTTGTCG+GGG - chr2.4:44175149-44175168 None:intergenic 55.0%
TGCCCTTGGTTGAGACCTGA+TGG + chr2.4:44175582-44175601 MS.gene76794:CDS 55.0%
TTCGTCATCGGTGGTCCTCA+CGG + chr2.4:44175813-44175832 MS.gene76794:CDS 55.0%
CCGATGACGAAACGGCCTGA+AGG - chr2.4:44175804-44175823 None:intergenic 60.0%
GTTAGACCAGCGTCACCGTG+AGG - chr2.4:44175831-44175850 None:intergenic 60.0%
TACGGTGGTTGGGGTGCACA+CGG + chr2.4:44175876-44175895 MS.gene76794:CDS 60.0%
TCAGGCCGTTTCGTCATCGG+TGG + chr2.4:44175804-44175823 MS.gene76794:CDS 60.0%
! CACGGTGACGCTGGTCTAAC+CGG + chr2.4:44175831-44175850 MS.gene76794:CDS 60.0%
! CACGGTGGTGGTGCTTTCTC+CGG + chr2.4:44175894-44175913 MS.gene76794:CDS 60.0%
GTGTGCACCCCAACCACCGT+AGG - chr2.4:44175877-44175896 None:intergenic 65.0%
GGTGGTCCTCACGGTGACGC+TGG + chr2.4:44175822-44175841 MS.gene76794:CDS 70.0%
GGTGGTTGGGGTGCACACGG+TGG + chr2.4:44175879-44175898 MS.gene76794:CDS 70.0%
GGTTGGGGTGCACACGGTGG+TGG + chr2.4:44175882-44175901 MS.gene76794:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 44175096 44176286 44175096 ID=MS.gene76794
chr2.4 mRNA 44175096 44176286 44175096 ID=MS.gene76794.t1;Parent=MS.gene76794
chr2.4 exon 44175096 44176286 44175096 ID=MS.gene76794.t1.exon1;Parent=MS.gene76794.t1
chr2.4 CDS 44175096 44176286 44175096 ID=cds.MS.gene76794.t1;Parent=MS.gene76794.t1
Gene Sequence

>MS.gene76794

ATGGCAACAGAAACTTTCCTATACACATCTGAATCTGTGAACGAGGGACACCCCGACAAGCTTTGCGACCAGATCTCTGATGCCGTTCTTGATGCTTGCCTTGAGCAAGATCCAGACAGCAAAGTTGCATGTGAAACTTGCACAAAGACCAACATGGTTATGGTCTTTGGTGAGATCACAACAAAAGCAAATGTTGACTATGAAAAGATTGTTCGTAACACATGCAGGAACATAGGTTTTGTTTCAGATGATGTTGGTCTTGATGCTGACAACTGCAAAGTCCTTGTCAACATTGAACAACAGAGTCCTGATATTGCTCAAGGTGTTCATGGCCATCTTACAAAAAGACCTGAGGAAATTGGTGCTGGTGATCAAGGTCACATGTTCGGGTATGCTACCGATGAAACACCTGAGCTTATGCCTTTAACCCATGTTCTTGCAACCAAGTTAGGAGCTAAGCTCACTGAAGTTCGCAAGAACGGAACATGCCCTTGGTTGAGACCTGATGGAAAGACCCAAGTCACTATTGAGTATTTCAATGACAAGGGTGCCATGGTTCCGATCCGTGTCCATACCGTCCTTATCTCAACACAGCACGACGAAACTGTGACCAATGATGAAATTGCAGCTGATTTGAAACAACATGTGATCAAGCCTGTGATCCCAGAGAAGTACCTTGATGAGAAAACCATCTTCCACTTGAACCCTTCAGGCCGTTTCGTCATCGGTGGTCCTCACGGTGACGCTGGTCTAACCGGCCGCAAGATCATCATCGATACCTACGGTGGTTGGGGTGCACACGGTGGTGGTGCTTTCTCCGGTAAAGATCCAACAAAAGTCGACAGGAGTGGAGCTTACATCGTGAGACAAGCTGCAAAGAGTATTGTTGCTAATGGACTTGCTAGAAGGTGCATTGTGCAAGTTTCCTATGCTATCGGTGTTCCAGAGCCATTGTCCGTGTTTGTTGATTCTTACGGTACCGGAAAGATCCCTGATAAGGAAATCCTTAACATTGTGAAAGAAACCTTTGACTTCAGGCCAGGAATGATCTCTATCAACCTTGATCTTAAGAGGGGTGGAAATAACAGGTTTTTGAAGACTGCTGCTTATGGACATTTTGGTAGAGATGATGCTGATTTCACATGGGAAGTTGTGAAGCCACTCAAGGGTGGAAAGTTAGCCACTGCCTAA
Protein sequence

>MS.gene76794.t1

MATETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYFNDKGAMVPIRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILNIVKETFDFRPGMISINLDLKRGGNNRFLKTAAYGHFGRDDADFTWEVVKPLKGGKLATA