AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76991

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76991.t1 MTR_8g087770 96.012 677 24 2 1 677 1 674 0.0 1311
MS.gene76991.t1 MTR_8g087750 81.602 674 112 6 6 674 11 677 0.0 1097
MS.gene76991.t1 MTR_8g087763 76.316 228 51 2 374 601 4 228 8.88e-120 361
MS.gene76991.t1 MTR_4g093750 34.080 402 238 13 67 452 35 425 1.45e-42 164
MS.gene76991.t1 MTR_4g093750 34.158 404 237 14 67 452 35 427 1.70e-41 162
MS.gene76991.t1 MTR_2g009690 32.170 401 255 10 63 452 40 434 3.97e-40 158
MS.gene76991.t1 MTR_1g032550 35.336 283 163 7 69 337 35 311 6.13e-33 135
MS.gene76991.t1 MTR_8g105580 35.461 282 163 6 69 337 35 310 9.83e-33 134
MS.gene76991.t1 MTR_8g105580 35.461 282 163 6 69 337 35 310 1.11e-32 134
MS.gene76991.t1 MTR_5g009150 29.741 464 298 14 69 518 35 484 7.88e-32 131
MS.gene76991.t1 MTR_7g451010 35.274 292 165 8 69 346 40 321 1.86e-31 130
MS.gene76991.t1 MTR_8g099550 33.438 317 185 9 69 369 34 340 6.10e-31 129
MS.gene76991.t1 MTR_4g085860 75.641 78 18 1 206 282 2 79 6.24e-31 116
MS.gene76991.t1 MTR_8g009510 34.494 316 183 9 69 369 40 346 1.02e-30 128
MS.gene76991.t1 MTR_8g027675 41.709 199 105 5 69 264 34 224 1.20e-30 127
MS.gene76991.t1 MTR_4g098920 34.169 319 180 10 69 369 34 340 4.64e-30 126
MS.gene76991.t1 MTR_6g045107 74.359 78 19 1 206 282 2 79 6.23e-29 110
MS.gene76991.t1 MTR_8g009510 30.488 246 151 6 136 369 2 239 3.33e-16 82.4
MS.gene76991.t1 MTR_8g463270 30.303 231 142 8 54 269 27 253 1.41e-15 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76991.t1 AT1G60500 55.621 676 282 6 3 670 2 667 0.0 750
MS.gene76991.t1 AT1G60530 71.379 290 75 1 18 307 19 300 2.19e-141 415
MS.gene76991.t1 AT4G33650 30.858 431 274 12 37 452 31 452 9.38e-41 160
MS.gene76991.t1 AT4G33650 30.858 431 274 12 37 452 31 452 9.69e-41 160
MS.gene76991.t1 AT2G14120 26.286 525 358 11 25 530 1 515 1.72e-39 155
MS.gene76991.t1 AT2G14120 28.859 447 289 11 25 452 1 437 3.38e-39 155
MS.gene76991.t1 AT2G14120 26.286 525 358 11 25 530 1 515 3.41e-39 155
MS.gene76991.t1 AT2G14120 31.742 356 218 8 25 362 1 349 2.36e-38 152
MS.gene76991.t1 AT1G14830 34.892 278 171 5 69 337 35 311 1.41e-34 140
MS.gene76991.t1 AT5G42080 29.374 463 301 11 69 518 34 483 9.84e-32 131
MS.gene76991.t1 AT5G42080 29.374 463 301 11 69 518 34 483 9.84e-32 131
MS.gene76991.t1 AT5G42080 33.548 310 194 6 69 369 34 340 1.08e-31 128
MS.gene76991.t1 AT3G60190 32.903 310 196 6 69 369 40 346 1.12e-31 131
MS.gene76991.t1 AT5G42080 33.548 310 194 6 69 369 34 340 2.84e-31 129
MS.gene76991.t1 AT2G44590 32.639 288 176 7 69 345 35 315 9.05e-31 128
MS.gene76991.t1 AT3G61760 41.176 204 110 5 69 268 34 231 4.80e-30 124
MS.gene76991.t1 AT3G61760 41.176 204 110 5 69 268 34 231 5.13e-30 126
MS.gene76991.t1 AT3G61760 41.176 204 110 5 69 268 34 231 7.03e-30 125
MS.gene76991.t1 AT2G44590 29.514 288 168 8 69 345 35 298 2.12e-21 99.4
MS.gene76991.t1 AT2G44590 29.514 288 168 8 69 345 35 298 2.15e-21 99.4
MS.gene76991.t1 AT1G53140 27.731 238 156 8 44 269 36 269 2.43e-14 77.4

Find 123 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGAGTCTCCATCTATTTC+TGG 0.166668 6.1:-56990453 MS.gene76991:CDS
CATTGTTGGTATTCCTGTTT+TGG 0.198486 6.1:-56991456 MS.gene76991:CDS
GCCTGCCAATTTGACTTCTT+TGG 0.199436 6.1:-56991324 MS.gene76991:CDS
GCAGTTCTTGGCATGAAATT+TGG 0.208693 6.1:+56991059 None:intergenic
TTAGTACTTGTGAGTCTATT+AGG 0.242558 6.1:-56991706 MS.gene76991:CDS
ATCAAGTTTGTTTGCCTTTG+TGG 0.244719 6.1:+56992342 None:intergenic
CTTGAGGAAGCTAAGTTAAT+TGG 0.255531 6.1:-56991086 MS.gene76991:CDS
AATGTTTGAAAAGCAGTTCT+TGG 0.267563 6.1:+56991047 None:intergenic
TTTGAGAAAGATTCTTCTAA+TGG 0.285565 6.1:-56991252 MS.gene76991:CDS
TGACAATGTGTGGAGCTATT+TGG 0.298324 6.1:-56990976 MS.gene76991:CDS
ATCTGATCATAAATGTTATC+AGG 0.304162 6.1:+56991803 None:intergenic
TAACATTTATGATCAGATTA+AGG 0.317964 6.1:-56991798 MS.gene76991:CDS
CTTCCAACCATTGTTGTTGT+TGG 0.322456 6.1:-56992160 MS.gene76991:CDS
TGATGATGTTAACATTGGTT+TGG 0.323376 6.1:-56991588 MS.gene76991:CDS
AGAGACTGTGGCCAACATTA+TGG 0.346194 6.1:-56990382 MS.gene76991:CDS
TTATACTCCAATACAAGTTC+TGG 0.358296 6.1:+56992013 None:intergenic
GCTCATTGATAGTATTGCTT+TGG 0.360639 6.1:-56990565 MS.gene76991:CDS
CAAGGGTACCTTTGGTTATG+AGG 0.366182 6.1:-56992060 MS.gene76991:CDS
CAATGCATTTGTTTATCATC+AGG 0.370755 6.1:+56991212 None:intergenic
CTTACTATGGTTGATCTTCC+AGG 0.371036 6.1:-56991854 MS.gene76991:CDS
TGCAAACTATTACCAGTTGC+AGG 0.374084 6.1:-56990919 MS.gene76991:CDS
ATCTGCCAAAGAAGTCAAAT+TGG 0.374655 6.1:+56991319 None:intergenic
TGGCGTTCCTGACCTTACTA+TGG 0.376759 6.1:-56991867 MS.gene76991:CDS
TCAAGTACTGTGAAGCTTGA+AGG 0.378498 6.1:-56990699 MS.gene76991:CDS
TTGAGAAAGATTCTTCTAAT+GGG 0.379749 6.1:-56991251 MS.gene76991:CDS
ACTTTCTCCAACAAACCTTC+AGG 0.384947 6.1:+56991617 None:intergenic
GATGATGTTAACATTGGTTT+GGG 0.391771 6.1:-56991587 MS.gene76991:CDS
ATAACTCGTGTTCCTGTTCA+TGG 0.396246 6.1:-56991830 MS.gene76991:CDS
TTGATCTTCTTAATTATCTC+AGG 0.396848 6.1:+56991386 None:intergenic
TCACAGATAGTTGATAAAAC+TGG 0.398319 6.1:-56991680 MS.gene76991:CDS
AGGCAAGATTAATTGCCTAT+TGG 0.399341 6.1:-56990605 MS.gene76991:CDS
AAGATTGACAAATCCATTGT+TGG 0.402415 6.1:-56991470 MS.gene76991:CDS
TCCGATCAATGTGTTGAATC+AGG 0.406853 6.1:-56990640 MS.gene76991:CDS
TTGAGGATAATGCTCTCTTC+AGG 0.409426 6.1:+56991752 None:intergenic
ATGGACAGGATTGGAGTCTA+TGG 0.410033 6.1:-56990363 MS.gene76991:CDS
GAATCAGGCCTTTGACTTGA+AGG 0.413968 6.1:-56990625 MS.gene76991:CDS
TTGATGCTCTTGAAAATCTA+AGG 0.416842 6.1:-56992213 MS.gene76991:CDS
GAACTTGTATTGGAGTATAA+TGG 0.418501 6.1:-56992010 MS.gene76991:CDS
TGTTATGCAAGCTGTTGATA+TGG 0.422633 6.1:-56990835 MS.gene76991:CDS
TTGTAATCCGGAGTACTTGC+AGG 0.423704 6.1:-56990793 MS.gene76991:CDS
AATTTGTACAAGGGTACCTT+TGG 0.424069 6.1:-56992068 MS.gene76991:CDS
TGTCTCTTACCAGAAATAGA+TGG 0.425419 6.1:+56990444 None:intergenic
GGACTACGATCTCTTCCATT+AGG 0.434291 6.1:+56991108 None:intergenic
TAAATCACCTGAAGGTTTGT+TGG 0.440077 6.1:-56991624 MS.gene76991:CDS
TTTGGAAAGGGTTGTTATCT+CGG 0.442091 6.1:-56990958 MS.gene76991:CDS
CCTCAGCACAATCTTCCAAT+AGG 0.443004 6.1:+56990590 None:intergenic
TCCACAGCCAGAACTTGTAT+TGG 0.443641 6.1:-56992020 MS.gene76991:CDS
TCATTATAAGAAGATACAAT+TGG 0.444707 6.1:+56992253 None:intergenic
TTCTACAAAGGATTTCCTAA+TGG 0.446044 6.1:-56991123 MS.gene76991:CDS
GTTCTGGCTGTGGAAGTAGA+TGG 0.452578 6.1:+56992029 None:intergenic
ACAGCTACTGAAGAGCTTGC+TGG 0.453969 6.1:-56991941 MS.gene76991:CDS
ACATTGTCAACAAAATTAGT+TGG 0.454264 6.1:+56990990 None:intergenic
GCATTGTACTGCTCGGTTAG+TGG 0.455271 6.1:-56991195 MS.gene76991:CDS
TACGAGTAGCAACCTGCAAC+TGG 0.461514 6.1:+56990907 None:intergenic
TGATAGCAGCTAATGATTCA+AGG 0.467025 6.1:+56992110 None:intergenic
TTCATTTCTAGCTTCTTCAT+AGG 0.469616 6.1:+56991529 None:intergenic
ACAAGCTTCTGAGCCAAAAC+AGG 0.473524 6.1:+56991443 None:intergenic
GTTGTTGGAGATCAATCATC+TGG 0.485196 6.1:-56992145 MS.gene76991:CDS
TGGAGTGTTTGAAATCCCTT+TGG 0.490204 6.1:+56991913 None:intergenic
TTATCAGGTTGACCATGAAC+AGG 0.495576 6.1:+56991818 None:intergenic
CACGGATTACACTTGTAATC+CGG 0.499086 6.1:-56990805 MS.gene76991:CDS
GAGCTTGCTGGAACTGCCAA+AGG 0.500559 6.1:-56991929 MS.gene76991:CDS
CAACAACAATGGTTGGAAGC+TGG 0.505287 6.1:+56992164 None:intergenic
GCGTCTCAACATTGCTAAAG+AGG 0.507977 6.1:-56992191 MS.gene76991:CDS
AGCTTGCATAACATGTTGAA+TGG 0.508185 6.1:+56990845 None:intergenic
ACAATTGGTGCAATAGATAG+AGG 0.511162 6.1:+56992268 None:intergenic
GCCAAAGAAGTCAAATTGGC+AGG 0.515228 6.1:+56991323 None:intergenic
TTTGTGTGAGAAATCGCATT+GGG 0.515555 6.1:-56991559 MS.gene76991:CDS
GTAAGAGACAAAGTTTAAGC+AGG 0.516534 6.1:-56990431 MS.gene76991:CDS
GGTGCAATAGATAGAGGTTG+AGG 0.516825 6.1:+56992274 None:intergenic
AATGCATTTGTTTATCATCA+GGG 0.517273 6.1:+56991213 None:intergenic
TCCAATCCTGTCCATAATGT+TGG 0.522411 6.1:+56990371 None:intergenic
CTGGAAGATCAACCATAGTA+AGG 0.523030 6.1:+56991855 None:intergenic
AGATCAACCATAGTAAGGTC+AGG 0.534194 6.1:+56991860 None:intergenic
TAATCTTGCCTTCAAGTCAA+AGG 0.534214 6.1:+56990617 None:intergenic
GCCAACATTATGGACAGGAT+TGG 0.534644 6.1:-56990372 MS.gene76991:CDS
GCCTGATTCAACACATTGAT+CGG 0.536471 6.1:+56990639 None:intergenic
ATCAGCTTGCCACGTGGACA+AGG 0.537919 6.1:-56992091 MS.gene76991:CDS
AAGGAGATTTGTAATGAACT+TGG 0.538785 6.1:-56990498 MS.gene76991:CDS
TCAGATTAAGGATATTATCA+TGG 0.543580 6.1:-56991786 MS.gene76991:CDS
ACTTTGATTGTGAAGAAGAA+TGG 0.548085 6.1:-56991887 MS.gene76991:CDS
ATGTGTGGAGCTATTTGGAA+AGG 0.549884 6.1:-56990971 MS.gene76991:CDS
AACAAATGCATTGTACTGCT+CGG 0.550416 6.1:-56991202 MS.gene76991:CDS
TCCAATACAAGTTCTGGCTG+TGG 0.550764 6.1:+56992019 None:intergenic
TCTCCAACAACAACAATGGT+TGG 0.551269 6.1:+56992157 None:intergenic
TTATATTCCTGCAAGTACTC+CGG 0.551776 6.1:+56990786 None:intergenic
GAGATTTGTAATGAACTTGG+TGG 0.560761 6.1:-56990495 MS.gene76991:CDS
TTCTTCACAATCAAAGTCAG+TGG 0.564699 6.1:+56991893 None:intergenic
TAGTTGATAAAACTGGTTTG+AGG 0.569831 6.1:-56991673 MS.gene76991:CDS
CTGTGGCCAACATTATGGAC+AGG 0.573065 6.1:-56990377 MS.gene76991:CDS
GACATTAGCTGTTGTAACAA+AGG 0.576294 6.1:-56991651 MS.gene76991:CDS
ATTACTTCAGAGAAAAGTGA+AGG 0.577066 6.1:-56991027 MS.gene76991:CDS
AGCTTGCTGGAACTGCCAAA+GGG 0.584621 6.1:-56991928 MS.gene76991:CDS
GTGGACAAGGAATTTGTACA+AGG 0.592993 6.1:-56992078 MS.gene76991:CDS
CAGTGTTGAGAGGCTGCTTG+AGG 0.594487 6.1:-56990472 MS.gene76991:CDS
CGTCTCAACATTGCTAAAGA+GGG 0.595388 6.1:-56992190 MS.gene76991:CDS
GGAAGAGATCGTAGTCCTTG+AGG 0.596070 6.1:-56991102 MS.gene76991:CDS
ATTGGAAGATTGTGCTGAGG+AGG 0.597029 6.1:-56990587 MS.gene76991:CDS
TGTGAGAAATCGCATTGGGG+AGG 0.608396 6.1:-56991555 MS.gene76991:CDS
GGTTGCTACTCGTAGAGCTG+TGG 0.612719 6.1:-56990898 MS.gene76991:CDS
ACAGGAACACGAGTTATACC+TGG 0.615115 6.1:+56991836 None:intergenic
TGTGTGGAGCTATTTGGAAA+GGG 0.616400 6.1:-56990970 MS.gene76991:CDS
CAATTAACTTAGCTTCCTCA+AGG 0.618556 6.1:+56991087 None:intergenic
CAAAACAGGAATACCAACAA+TGG 0.623000 6.1:+56991457 None:intergenic
ACTGCTGATGATGTTAACAT+TGG 0.625783 6.1:-56991593 MS.gene76991:CDS
AAGGCTGATAAATCACCTGA+AGG 0.627415 6.1:-56991632 MS.gene76991:CDS
TGCTGAAAGTGATTCTACAA+AGG 0.631584 6.1:-56991135 MS.gene76991:CDS
GATTAACAAAGATCTGCAGA+AGG 0.642625 6.1:-56990517 MS.gene76991:CDS
TTGATCTCCAACAACAACAA+TGG 0.648908 6.1:+56992153 None:intergenic
TAGAGAAAGCAAAGAGACTG+TGG 0.654791 6.1:-56990394 MS.gene76991:CDS
GTACAAATTCCTTGTCCACG+TGG 0.660130 6.1:+56992082 None:intergenic
TTACTTCAGAGAAAAGTGAA+GGG 0.662697 6.1:-56991026 MS.gene76991:CDS
TGTTGATATGGAGAAGCACA+CGG 0.665028 6.1:-56990823 MS.gene76991:CDS
AACTTGGTGGCAGTGTTGAG+AGG 0.665158 6.1:-56990482 MS.gene76991:CDS
CAGTAGCAGAAAGAACATTG+AGG 0.676780 6.1:+56991735 None:intergenic
GTTTGTGTGAGAAATCGCAT+TGG 0.677044 6.1:-56991560 MS.gene76991:CDS
GCTGCTATCAGCTTGCCACG+TGG 0.703866 6.1:-56992097 MS.gene76991:CDS
GTTGATCATCAACATCAACA+TGG 0.706155 6.1:+56992296 None:intergenic
TGGAAACAAGAGTTCCACAA+AGG 0.707464 6.1:-56992356 MS.gene76991:CDS
CCTATTGGAAGATTGTGCTG+AGG 0.716472 6.1:-56990590 MS.gene76991:CDS
TGGACAAGGAATTTGTACAA+GGG 0.728664 6.1:-56992077 MS.gene76991:CDS
TTGTGTGAGAAATCGCATTG+GGG 0.740526 6.1:-56991558 MS.gene76991:CDS
ACTGGTAATAGTTTGCAGAG+TGG 0.741343 6.1:+56990925 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAATTTTTGAAAAAAGTTT+TGG - chr6.1:56991975-56991994 MS.gene76991:CDS 15.0%
!! TAACATTTATGATCAGATTA+AGG - chr6.1:56990919-56990938 MS.gene76991:CDS 20.0%
!! TCATTATAAGAAGATACAAT+TGG + chr6.1:56990467-56990486 None:intergenic 20.0%
! AATGCATTTGTTTATCATCA+GGG + chr6.1:56991507-56991526 None:intergenic 25.0%
! ACATTGTCAACAAAATTAGT+TGG + chr6.1:56991730-56991749 None:intergenic 25.0%
! ATCTGATCATAAATGTTATC+AGG + chr6.1:56990917-56990936 None:intergenic 25.0%
! TCAGATTAAGGATATTATCA+TGG - chr6.1:56990931-56990950 MS.gene76991:CDS 25.0%
! TTGAGAAAGATTCTTCTAAT+GGG - chr6.1:56991466-56991485 MS.gene76991:CDS 25.0%
! TTGATCTTCTTAATTATCTC+AGG + chr6.1:56991334-56991353 None:intergenic 25.0%
! TTTGAGAAAGATTCTTCTAA+TGG - chr6.1:56991465-56991484 MS.gene76991:CDS 25.0%
AAGATTGACAAATCCATTGT+TGG - chr6.1:56991247-56991266 MS.gene76991:CDS 30.0%
AAGGAGATTTGTAATGAACT+TGG - chr6.1:56992219-56992238 MS.gene76991:CDS 30.0%
AATGTTTGAAAAGCAGTTCT+TGG + chr6.1:56991673-56991692 None:intergenic 30.0%
ACTTTGATTGTGAAGAAGAA+TGG - chr6.1:56990830-56990849 MS.gene76991:CDS 30.0%
ATTACTTCAGAGAAAAGTGA+AGG - chr6.1:56991690-56991709 MS.gene76991:CDS 30.0%
CAATGCATTTGTTTATCATC+AGG + chr6.1:56991508-56991527 None:intergenic 30.0%
GAACTTGTATTGGAGTATAA+TGG - chr6.1:56990707-56990726 MS.gene76991:CDS 30.0%
TTACTTCAGAGAAAAGTGAA+GGG - chr6.1:56991691-56991710 MS.gene76991:CDS 30.0%
TTAGTACTTGTGAGTCTATT+AGG - chr6.1:56991011-56991030 MS.gene76991:CDS 30.0%
TTATACTCCAATACAAGTTC+TGG + chr6.1:56990707-56990726 None:intergenic 30.0%
TTCTACAAAGGATTTCCTAA+TGG - chr6.1:56991594-56991613 MS.gene76991:CDS 30.0%
TTGATGCTCTTGAAAATCTA+AGG - chr6.1:56990504-56990523 MS.gene76991:CDS 30.0%
! CTAATTTTGTTGACAATGTG+TGG - chr6.1:56991731-56991750 MS.gene76991:CDS 30.0%
! TGATGATGTTAACATTGGTT+TGG - chr6.1:56991129-56991148 MS.gene76991:CDS 30.0%
!! GATGATGTTAACATTGGTTT+GGG - chr6.1:56991130-56991149 MS.gene76991:CDS 30.0%
!! TAGTTGATAAAACTGGTTTG+AGG - chr6.1:56991044-56991063 MS.gene76991:CDS 30.0%
!! TCACAGATAGTTGATAAAAC+TGG - chr6.1:56991037-56991056 MS.gene76991:CDS 30.0%
!! TTCATTTCTAGCTTCTTCAT+AGG + chr6.1:56991191-56991210 None:intergenic 30.0%
AACAAATGCATTGTACTGCT+CGG - chr6.1:56991515-56991534 MS.gene76991:CDS 35.0%
ACAATTGGTGCAATAGATAG+AGG + chr6.1:56990452-56990471 None:intergenic 35.0%
AGCTTGCATAACATGTTGAA+TGG + chr6.1:56991875-56991894 None:intergenic 35.0%
AGGCAAGATTAATTGCCTAT+TGG - chr6.1:56992112-56992131 MS.gene76991:CDS 35.0%
ATCTGCCAAAGAAGTCAAAT+TGG + chr6.1:56991401-56991420 None:intergenic 35.0%
CAAAACAGGAATACCAACAA+TGG + chr6.1:56991263-56991282 None:intergenic 35.0%
CAATTAACTTAGCTTCCTCA+AGG + chr6.1:56991633-56991652 None:intergenic 35.0%
CTTGAGGAAGCTAAGTTAAT+TGG - chr6.1:56991631-56991650 MS.gene76991:CDS 35.0%
GACATTAGCTGTTGTAACAA+AGG - chr6.1:56991066-56991085 MS.gene76991:CDS 35.0%
GAGATTTGTAATGAACTTGG+TGG - chr6.1:56992222-56992241 MS.gene76991:CDS 35.0%
GATTAACAAAGATCTGCAGA+AGG - chr6.1:56992200-56992219 MS.gene76991:CDS 35.0%
GTAAGAGACAAAGTTTAAGC+AGG - chr6.1:56992286-56992305 MS.gene76991:CDS 35.0%
GTTGATCATCAACATCAACA+TGG + chr6.1:56990424-56990443 None:intergenic 35.0%
TAAATCACCTGAAGGTTTGT+TGG - chr6.1:56991093-56991112 MS.gene76991:CDS 35.0%
TAATCTTGCCTTCAAGTCAA+AGG + chr6.1:56992103-56992122 None:intergenic 35.0%
TGATAGCAGCTAATGATTCA+AGG + chr6.1:56990610-56990629 None:intergenic 35.0%
TGCTGAAAGTGATTCTACAA+AGG - chr6.1:56991582-56991601 MS.gene76991:CDS 35.0%
TGGACAAGGAATTTGTACAA+GGG - chr6.1:56990640-56990659 MS.gene76991:CDS 35.0%
TGTCTCTTACCAGAAATAGA+TGG + chr6.1:56992276-56992295 None:intergenic 35.0%
TGTTATGCAAGCTGTTGATA+TGG - chr6.1:56991882-56991901 MS.gene76991:CDS 35.0%
TTATATTCCTGCAAGTACTC+CGG + chr6.1:56991934-56991953 None:intergenic 35.0%
TTCTTCACAATCAAAGTCAG+TGG + chr6.1:56990827-56990846 None:intergenic 35.0%
TTGATCTCCAACAACAACAA+TGG + chr6.1:56990567-56990586 None:intergenic 35.0%
TTTGGAAAGGGTTGTTATCT+CGG - chr6.1:56991759-56991778 MS.gene76991:CDS 35.0%
TTTGTGTGAGAAATCGCATT+GGG - chr6.1:56991158-56991177 MS.gene76991:CDS 35.0%
! ACTGCTGATGATGTTAACAT+TGG - chr6.1:56991124-56991143 MS.gene76991:CDS 35.0%
! ATCAAGTTTGTTTGCCTTTG+TGG + chr6.1:56990378-56990397 None:intergenic 35.0%
! CATTGTTGGTATTCCTGTTT+TGG - chr6.1:56991261-56991280 MS.gene76991:CDS 35.0%
! GCTCATTGATAGTATTGCTT+TGG - chr6.1:56992152-56992171 MS.gene76991:CDS 35.0%
!! AATTTGTACAAGGGTACCTT+TGG - chr6.1:56990649-56990668 MS.gene76991:CDS 35.0%
AAGGCTGATAAATCACCTGA+AGG - chr6.1:56991085-56991104 MS.gene76991:CDS 40.0%
ACTTTCTCCAACAAACCTTC+AGG + chr6.1:56991103-56991122 None:intergenic 40.0%
AGATCAACCATAGTAAGGTC+AGG + chr6.1:56990860-56990879 None:intergenic 40.0%
ATAACTCGTGTTCCTGTTCA+TGG - chr6.1:56990887-56990906 MS.gene76991:CDS 40.0%
ATGTGTGGAGCTATTTGGAA+AGG - chr6.1:56991746-56991765 MS.gene76991:CDS 40.0%
CAGTAGCAGAAAGAACATTG+AGG + chr6.1:56990985-56991004 None:intergenic 40.0%
CGTCTCAACATTGCTAAAGA+GGG - chr6.1:56990527-56990546 MS.gene76991:CDS 40.0%
CTGGAAGATCAACCATAGTA+AGG + chr6.1:56990865-56990884 None:intergenic 40.0%
CTTACTATGGTTGATCTTCC+AGG - chr6.1:56990863-56990882 MS.gene76991:CDS 40.0%
GCAGTTCTTGGCATGAAATT+TGG + chr6.1:56991661-56991680 None:intergenic 40.0%
GCCTGATTCAACACATTGAT+CGG + chr6.1:56992081-56992100 None:intergenic 40.0%
GTGGACAAGGAATTTGTACA+AGG - chr6.1:56990639-56990658 MS.gene76991:CDS 40.0%
GTTGTTGGAGATCAATCATC+TGG - chr6.1:56990572-56990591 MS.gene76991:CDS 40.0%
GTTTGTGTGAGAAATCGCAT+TGG - chr6.1:56991157-56991176 MS.gene76991:CDS 40.0%
TAGAGAAAGCAAAGAGACTG+TGG - chr6.1:56992323-56992342 MS.gene76991:CDS 40.0%
TCCAATCCTGTCCATAATGT+TGG + chr6.1:56992349-56992368 None:intergenic 40.0%
TCCGATCAATGTGTTGAATC+AGG - chr6.1:56992077-56992096 MS.gene76991:CDS 40.0%
TCTCCAACAACAACAATGGT+TGG + chr6.1:56990563-56990582 None:intergenic 40.0%
TGACAATGTGTGGAGCTATT+TGG - chr6.1:56991741-56991760 MS.gene76991:CDS 40.0%
TGCAAACTATTACCAGTTGC+AGG - chr6.1:56991798-56991817 MS.gene76991:CDS 40.0%
TGGAAACAAGAGTTCCACAA+AGG - chr6.1:56990361-56990380 MS.gene76991:CDS 40.0%
TGTGTGGAGCTATTTGGAAA+GGG - chr6.1:56991747-56991766 MS.gene76991:CDS 40.0%
TTGAGGATAATGCTCTCTTC+AGG + chr6.1:56990968-56990987 None:intergenic 40.0%
TTGTGTGAGAAATCGCATTG+GGG - chr6.1:56991159-56991178 MS.gene76991:CDS 40.0%
! ACTGGTAATAGTTTGCAGAG+TGG + chr6.1:56991795-56991814 None:intergenic 40.0%
! CACGGATTACACTTGTAATC+CGG - chr6.1:56991912-56991931 MS.gene76991:CDS 40.0%
! CTTCCAACCATTGTTGTTGT+TGG - chr6.1:56990557-56990576 MS.gene76991:CDS 40.0%
! TCAAGTACTGTGAAGCTTGA+AGG - chr6.1:56992018-56992037 MS.gene76991:CDS 40.0%
! TGGAGTGTTTGAAATCCCTT+TGG + chr6.1:56990807-56990826 None:intergenic 40.0%
! TGTTGATATGGAGAAGCACA+CGG - chr6.1:56991894-56991913 MS.gene76991:CDS 40.0%
! TTTTGCAGCCTCATAACCAA+AGG + chr6.1:56990668-56990687 None:intergenic 40.0%
!! TTATCAGGTTGACCATGAAC+AGG + chr6.1:56990902-56990921 None:intergenic 40.0%
!!! TTTTCAAGAGCATCAAGCAC+TGG + chr6.1:56990500-56990519 None:intergenic 40.0%
ACAAGCTTCTGAGCCAAAAC+AGG + chr6.1:56991277-56991296 None:intergenic 45.0%
ACAGGAACACGAGTTATACC+TGG + chr6.1:56990884-56990903 None:intergenic 45.0%
AGAGACTGTGGCCAACATTA+TGG - chr6.1:56992335-56992354 MS.gene76991:CDS 45.0%
ATGGACAGGATTGGAGTCTA+TGG - chr6.1:56992354-56992373 MS.gene76991:CDS 45.0%
ATTGGAAGATTGTGCTGAGG+AGG - chr6.1:56992130-56992149 MS.gene76991:CDS 45.0%
CAACAACAATGGTTGGAAGC+TGG + chr6.1:56990556-56990575 None:intergenic 45.0%
CCTATTGGAAGATTGTGCTG+AGG - chr6.1:56992127-56992146 MS.gene76991:CDS 45.0%
CCTCAGCACAATCTTCCAAT+AGG + chr6.1:56992130-56992149 None:intergenic 45.0%
GAGGAGTCTCCATCTATTTC+TGG - chr6.1:56992264-56992283 MS.gene76991:CDS 45.0%
GCCAAAGAAGTCAAATTGGC+AGG + chr6.1:56991397-56991416 None:intergenic 45.0%
GCCAACATTATGGACAGGAT+TGG - chr6.1:56992345-56992364 MS.gene76991:CDS 45.0%
GCGTCTCAACATTGCTAAAG+AGG - chr6.1:56990526-56990545 MS.gene76991:CDS 45.0%
GGACTACGATCTCTTCCATT+AGG + chr6.1:56991612-56991631 None:intergenic 45.0%
GGTGCAATAGATAGAGGTTG+AGG + chr6.1:56990446-56990465 None:intergenic 45.0%
GTACAAATTCCTTGTCCACG+TGG + chr6.1:56990638-56990657 None:intergenic 45.0%
TCCACAGCCAGAACTTGTAT+TGG - chr6.1:56990697-56990716 MS.gene76991:CDS 45.0%
! GCCTGCCAATTTGACTTCTT+TGG - chr6.1:56991393-56991412 MS.gene76991:CDS 45.0%
! TCCAATACAAGTTCTGGCTG+TGG + chr6.1:56990701-56990720 None:intergenic 45.0%
! TTGTAATCCGGAGTACTTGC+AGG - chr6.1:56991924-56991943 MS.gene76991:CDS 45.0%
!! CAAGGGTACCTTTGGTTATG+AGG - chr6.1:56990657-56990676 MS.gene76991:CDS 45.0%
!! GAATCAGGCCTTTGACTTGA+AGG - chr6.1:56992092-56992111 MS.gene76991:CDS 45.0%
ACAGCTACTGAAGAGCTTGC+TGG - chr6.1:56990776-56990795 MS.gene76991:CDS 50.0%
CTGTGGCCAACATTATGGAC+AGG - chr6.1:56992340-56992359 MS.gene76991:CDS 50.0%
GCATTGTACTGCTCGGTTAG+TGG - chr6.1:56991522-56991541 MS.gene76991:CDS 50.0%
GGAAGAGATCGTAGTCCTTG+AGG - chr6.1:56991615-56991634 MS.gene76991:CDS 50.0%
TACGAGTAGCAACCTGCAAC+TGG + chr6.1:56991813-56991832 None:intergenic 50.0%
TGGCGTTCCTGACCTTACTA+TGG - chr6.1:56990850-56990869 MS.gene76991:CDS 50.0%
TGTGAGAAATCGCATTGGGG+AGG - chr6.1:56991162-56991181 MS.gene76991:CDS 50.0%
! AACTTGGTGGCAGTGTTGAG+AGG - chr6.1:56992235-56992254 MS.gene76991:CDS 50.0%
! GTTCTGGCTGTGGAAGTAGA+TGG + chr6.1:56990691-56990710 None:intergenic 50.0%
!! AGCTTGCTGGAACTGCCAAA+GGG - chr6.1:56990789-56990808 MS.gene76991:CDS 50.0%
ATCAGCTTGCCACGTGGACA+AGG - chr6.1:56990626-56990645 MS.gene76991:CDS 55.0%
GGTTGCTACTCGTAGAGCTG+TGG - chr6.1:56991819-56991838 MS.gene76991:CDS 55.0%
! CAGTGTTGAGAGGCTGCTTG+AGG - chr6.1:56992245-56992264 MS.gene76991:CDS 55.0%
!! GAGCTTGCTGGAACTGCCAA+AGG - chr6.1:56990788-56990807 MS.gene76991:CDS 55.0%
GCTGCTATCAGCTTGCCACG+TGG - chr6.1:56990620-56990639 MS.gene76991:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 56990353 56992386 56990353 ID=MS.gene76991
chr6.1 mRNA 56990353 56992386 56990353 ID=MS.gene76991.t1;Parent=MS.gene76991
chr6.1 exon 56990353 56992386 56990353 ID=MS.gene76991.t1.exon1;Parent=MS.gene76991.t1
chr6.1 CDS 56990353 56992386 56990353 ID=cds.MS.gene76991.t1;Parent=MS.gene76991.t1
Gene Sequence

>MS.gene76991

ATGACAATTGGAAACAAGAGTTCCACAAAGGCAAACAAACTTGATGAATCATCATCACTTGCATTAGTCCATGTTGATGTTGATGATCAACCTCAACCTCTATCTATTGCACCAATTGTATCTTCTTATAATGAAAAGATACGTCCAGTGCTTGATGCTCTTGAAAATCTAAGGCGTCTCAACATTGCTAAAGAGGGTATCCAGCTTCCAACCATTGTTGTTGTTGGAGATCAATCATCTGGAAAGTCTAGTGTCCTTGAATCATTAGCTGCTATCAGCTTGCCACGTGGACAAGGAATTTGTACAAGGGTACCTTTGGTTATGAGGCTGCAAAACCATCTACTTCCACAGCCAGAACTTGTATTGGAGTATAATGGCAAACATGTTTTAACTGACGAAGCAAATGTCTCTGATGCAATAAACACAGCTACTGAAGAGCTTGCTGGAACTGCCAAAGGGATTTCAAACACTCCACTGACTTTGATTGTGAAGAAGAATGGCGTTCCTGACCTTACTATGGTTGATCTTCCAGGTATAACTCGTGTTCCTGTTCATGGTCAACCTGATAACATTTATGATCAGATTAAGGATATTATCATGGAGTATATAACTCCTGAAGAGAGCATTATCCTCAATGTTCTTTCTGCTACTGTCGATTTTAGTACTTGTGAGTCTATTAGGATGTCACAGATAGTTGATAAAACTGGTTTGAGGACATTAGCTGTTGTAACAAAGGCTGATAAATCACCTGAAGGTTTGTTGGAGAAAGTAACTGCTGATGATGTTAACATTGGTTTGGGCTATGTTTGTGTGAGAAATCGCATTGGGGAGGAATCCTATGAAGAAGCTAGAAATGAAGAACAGAAGTTGTTTGAGTCTCACTCACTTCTTTCTAAGATTGACAAATCCATTGTTGGTATTCCTGTTTTGGCTCAGAAGCTTGTTCATGTGCAAGCTATGATTATTTCAAAAACTTTGCCTGAGATAATTAAGAAGATCAATGAGAAGCTTTATAATAGTGTACATGAGTTGAAGACTTTGCCTGCCAATTTGACTTCTTTGGCAGATGCTATGTCTGCTTTTCTGCATATTGTTTCTCTGTCAAGAGATTCTTTGAGAAAGATTCTTCTAATGGGAGAATTTGAAGAATACCCTGATGATAAACAAATGCATTGTACTGCTCGGTTAGTGGAGATGTTGAATTCATATGCAAGTGATCTTCAAAACTGTGCTGAAAGTGATTCTACAAAGGATTTCCTAATGGAAGAGATCGTAGTCCTTGAGGAAGCTAAGTTAATTGGTCTTCCAAATTTCATGCCAAGAACTGCTTTTCAAACATTACTTCAGAGAAAAGTGAAGGGCATTTCTCATATGCCAACTAATTTTGTTGACAATGTGTGGAGCTATTTGGAAAGGGTTGTTATCTCGGTCATGAATCGCCACTCTGCAAACTATTACCAGTTGCAGGTTGCTACTCGTAGAGCTGTGGAGCATCTTATTGCTAAGAAGAAGAGCAATTCCATTCAACATGTTATGCAAGCTGTTGATATGGAGAAGCACACGGATTACACTTGTAATCCGGAGTACTTGCAGGAATATAACTTGCTGATGTCTAATCAAGAAGAATTTTTGAAAAAAGTTTTGGATGATCATCGTTGTCGATTTTCAAGTACTGTGAAGCTTGAAGGAGTTGATGACATTAAAGTTAATCATCTGATAGAGTATCCGATCAATGTGTTGAATCAGGCCTTTGACTTGAAGGCAAGATTAATTGCCTATTGGAAGATTGTGCTGAGGAGGCTCATTGATAGTATTGCTTTGGATTTGATGCTAAGCATCAATGAACTGATTAACAAAGATCTGCAGAAGGAGATTTGTAATGAACTTGGTGGCAGTGTTGAGAGGCTGCTTGAGGAGTCTCCATCTATTTCTGGTAAGAGACAAAGTTTAAGCAGGAGTGTGAAAGTTCTTAGAGAAAGCAAAGAGACTGTGGCCAACATTATGGACAGGATTGGAGTCTATGGTGATAACTAG
Protein sequence

>MS.gene76991.t1

MTIGNKSSTKANKLDESSSLALVHVDVDDQPQPLSIAPIVSSYNEKIRPVLDALENLRRLNIAKEGIQLPTIVVVGDQSSGKSSVLESLAAISLPRGQGICTRVPLVMRLQNHLLPQPELVLEYNGKHVLTDEANVSDAINTATEELAGTAKGISNTPLTLIVKKNGVPDLTMVDLPGITRVPVHGQPDNIYDQIKDIIMEYITPEESIILNVLSATVDFSTCESIRMSQIVDKTGLRTLAVVTKADKSPEGLLEKVTADDVNIGLGYVCVRNRIGEESYEEARNEEQKLFESHSLLSKIDKSIVGIPVLAQKLVHVQAMIISKTLPEIIKKINEKLYNSVHELKTLPANLTSLADAMSAFLHIVSLSRDSLRKILLMGEFEEYPDDKQMHCTARLVEMLNSYASDLQNCAESDSTKDFLMEEIVVLEEAKLIGLPNFMPRTAFQTLLQRKVKGISHMPTNFVDNVWSYLERVVISVMNRHSANYYQLQVATRRAVEHLIAKKKSNSIQHVMQAVDMEKHTDYTCNPEYLQEYNLLMSNQEEFLKKVLDDHRCRFSSTVKLEGVDDIKVNHLIEYPINVLNQAFDLKARLIAYWKIVLRRLIDSIALDLMLSINELINKDLQKEICNELGGSVERLLEESPSISGKRQSLSRSVKVLRESKETVANIMDRIGVYGDN