Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77445.t1 | XP_003613768.1 | 85.1 | 94 | 9 | 2 | 1 | 90 | 1 | 93 | 2.30E-19 | 104.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77445.t1 | G7KF81 | 85.1 | 94 | 9 | 2 | 1 | 90 | 1 | 93 | 1.7e-19 | 104.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049594 | MS.gene77445 | 0.816347 | 5.79E-52 | -1.69E-46 |
| MS.gene056297 | MS.gene77445 | -0.821462 | 3.99E-53 | -1.69E-46 |
| MS.gene059896 | MS.gene77445 | 0.867518 | 1.29E-65 | -1.69E-46 |
| MS.gene061114 | MS.gene77445 | 0.807802 | 4.23E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77445.t1 | MTR_5g040650 | 93.617 | 94 | 1 | 2 | 1 | 90 | 1 | 93 | 3.68e-52 | 164 |
| MS.gene77445.t1 | MTR_5g040650 | 93.617 | 94 | 1 | 2 | 1 | 90 | 1 | 93 | 5.45e-52 | 164 |
| MS.gene77445.t1 | MTR_8g467220 | 54.737 | 95 | 35 | 4 | 1 | 91 | 1 | 91 | 4.95e-21 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77445.t1 | AT3G47550 | 45.833 | 96 | 44 | 3 | 1 | 91 | 1 | 93 | 3.99e-15 | 68.2 |
| MS.gene77445.t1 | AT5G62460 | 86.111 | 36 | 5 | 0 | 56 | 91 | 67 | 102 | 5.25e-15 | 68.6 |
| MS.gene77445.t1 | AT5G62460 | 86.111 | 36 | 5 | 0 | 56 | 91 | 67 | 102 | 5.25e-15 | 68.6 |
| MS.gene77445.t1 | AT5G62460 | 86.111 | 36 | 5 | 0 | 56 | 91 | 80 | 115 | 5.84e-15 | 68.6 |
| MS.gene77445.t1 | AT3G47550 | 45.833 | 96 | 44 | 3 | 1 | 91 | 1 | 93 | 6.29e-15 | 67.8 |
| MS.gene77445.t1 | AT3G47550 | 45.833 | 96 | 44 | 3 | 1 | 91 | 1 | 93 | 1.79e-14 | 67.0 |
| MS.gene77445.t1 | AT3G47550 | 45.833 | 96 | 44 | 3 | 1 | 91 | 1 | 93 | 1.79e-14 | 67.0 |
Find 44 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGACAGTGTTTCCAATT+TGG | 0.230598 | 5.1:-30837361 | MS.gene77445:CDS |
| ACTGCTGATGCCGGATCATC+TGG | 0.359419 | 5.1:-30837462 | MS.gene77445:CDS |
| GGCACAGGGAGTCTCCAAAT+TGG | 0.387742 | 5.1:+30837347 | None:intergenic |
| TGTTGGTCAACAGGAACAAC+TGG | 0.394104 | 5.1:+30837534 | None:intergenic |
| GAAGGTGGTGGGCAGGTTGT+TGG | 0.414952 | 5.1:+30837517 | None:intergenic |
| TGGGCAGGTTGTTGGTCAAC+AGG | 0.434759 | 5.1:+30837525 | None:intergenic |
| TCTCCTGCCACTGCTGATGC+CGG | 0.451697 | 5.1:-30837471 | MS.gene77445:CDS |
| AATGATCGGAAGGAAGGTGG+TGG | 0.456132 | 5.1:+30837505 | None:intergenic |
| ACTACCAAGTGATCGCTCAT+TGG | 0.456166 | 5.1:+30837579 | None:intergenic |
| AGATACGGCACTCAACCATC+TGG | 0.480714 | 5.1:+30837391 | None:intergenic |
| GGTTGAGTGCCGTATCTGTC+AGG | 0.494067 | 5.1:-30837385 | MS.gene77445:CDS |
| CAGTGGCAGGAGATGGCGAT+GGG | 0.494476 | 5.1:+30837481 | None:intergenic |
| GATGCCGGATCATCTGGATC+TGG | 0.496615 | 5.1:-30837456 | MS.gene77445:CDS |
| TCGGAAGGAAGGTGGTGGGC+AGG | 0.502664 | 5.1:+30837510 | None:intergenic |
| GAGACTACCGCTACAGGCAC+AGG | 0.503593 | 5.1:+30837332 | None:intergenic |
| TACCTTGAGACTACCGCTAC+AGG | 0.507838 | 5.1:+30837326 | None:intergenic |
| GGAACCAGATCCAGATGATC+CGG | 0.527244 | 5.1:+30837452 | None:intergenic |
| GATCCGGCATCAGCAGTGGC+AGG | 0.529047 | 5.1:+30837468 | None:intergenic |
| GCATCAGCAGTGGCAGGAGA+TGG | 0.530163 | 5.1:+30837474 | None:intergenic |
| GGGAATGATCGGAAGGAAGG+TGG | 0.530407 | 5.1:+30837502 | None:intergenic |
| TTTCCCAATGAGCGATCACT+TGG | 0.540113 | 5.1:-30837583 | None:intergenic |
| GCAGTGGCAGGAGATGGCGA+TGG | 0.545980 | 5.1:+30837480 | None:intergenic |
| ATGGGGAATGATCGGAAGGA+AGG | 0.548616 | 5.1:+30837499 | None:intergenic |
| GAACAACTGGGTGGTCGAGT+TGG | 0.550257 | 5.1:+30837547 | None:intergenic |
| AGATGGCGATGGGGAATGAT+CGG | 0.552402 | 5.1:+30837491 | None:intergenic |
| TGTCTTCCTCCTGACAGATA+CGG | 0.567028 | 5.1:+30837376 | None:intergenic |
| TGCCTGTAGCGGTAGTCTCA+AGG | 0.574911 | 5.1:-30837328 | MS.gene77445:CDS |
| GGCGATGGGGAATGATCGGA+AGG | 0.583505 | 5.1:+30837495 | None:intergenic |
| ATCTGGATCTGGTTCCGCGG+TGG | 0.583717 | 5.1:-30837445 | MS.gene77445:CDS |
| CACTCAACCATCTGGATCAG+TGG | 0.593877 | 5.1:+30837399 | None:intergenic |
| ATGATCGGAAGGAAGGTGGT+GGG | 0.601522 | 5.1:+30837506 | None:intergenic |
| GGTTCCGCGGTGGATCGTGA+TGG | 0.608708 | 5.1:-30837435 | MS.gene77445:CDS |
| GGATCGTGATGGCGATGAGG+AGG | 0.616205 | 5.1:-30837424 | MS.gene77445:CDS |
| AGATGATCCGGCATCAGCAG+TGG | 0.627660 | 5.1:+30837464 | None:intergenic |
| AGACTACCGCTACAGGCACA+GGG | 0.636998 | 5.1:+30837333 | None:intergenic |
| GAGACTCCCTGTGCCTGTAG+CGG | 0.639323 | 5.1:-30837339 | MS.gene77445:CDS |
| ATCATCTGGATCTGGTTCCG+CGG | 0.646013 | 5.1:-30837448 | MS.gene77445:CDS |
| GGAGGAGCCACTGATCCAGA+TGG | 0.656675 | 5.1:-30837406 | MS.gene77445:CDS |
| GTTGGTCAACAGGAACAACT+GGG | 0.659884 | 5.1:+30837535 | None:intergenic |
| GGTGGATCGTGATGGCGATG+AGG | 0.679783 | 5.1:-30837427 | MS.gene77445:CDS |
| TGAGTGCCGTATCTGTCAGG+AGG | 0.681591 | 5.1:-30837382 | MS.gene77445:CDS |
| AGTGGCAGGAGATGGCGATG+GGG | 0.704357 | 5.1:+30837482 | None:intergenic |
| ATCGCCATCACGATCCACCG+CGG | 0.714224 | 5.1:+30837431 | None:intergenic |
| GGTCAACAGGAACAACTGGG+TGG | 0.749101 | 5.1:+30837538 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GGAAGACAGTGTTTCCAATT+TGG | - | chr5.1:30837523-30837542 | MS.gene77445:CDS | 40.0% | |
| GTTGGTCAACAGGAACAACT+GGG | + | chr5.1:30837352-30837371 | None:intergenic | 45.0% | |
| TGTCTTCCTCCTGACAGATA+CGG | + | chr5.1:30837511-30837530 | None:intergenic | 45.0% | |
| TGTTGGTCAACAGGAACAAC+TGG | + | chr5.1:30837353-30837372 | None:intergenic | 45.0% | |
| AATGATCGGAAGGAAGGTGG+TGG | + | chr5.1:30837382-30837401 | None:intergenic | 50.0% | |
| AGATACGGCACTCAACCATC+TGG | + | chr5.1:30837496-30837515 | None:intergenic | 50.0% | |
| AGATGGCGATGGGGAATGAT+CGG | + | chr5.1:30837396-30837415 | None:intergenic | 50.0% | |
| ATGATCGGAAGGAAGGTGGT+GGG | + | chr5.1:30837381-30837400 | None:intergenic | 50.0% | |
| ATGGGGAATGATCGGAAGGA+AGG | + | chr5.1:30837388-30837407 | None:intergenic | 50.0% | |
| CACTCAACCATCTGGATCAG+TGG | + | chr5.1:30837488-30837507 | None:intergenic | 50.0% | |
| GGAACCAGATCCAGATGATC+CGG | + | chr5.1:30837435-30837454 | None:intergenic | 50.0% | |
| TACCTTGAGACTACCGCTAC+AGG | + | chr5.1:30837561-30837580 | None:intergenic | 50.0% | |
| ! | ATCATCTGGATCTGGTTCCG+CGG | - | chr5.1:30837436-30837455 | MS.gene77445:CDS | 50.0% |
| ACTGCTGATGCCGGATCATC+TGG | - | chr5.1:30837422-30837441 | MS.gene77445:CDS | 55.0% | |
| AGACTACCGCTACAGGCACA+GGG | + | chr5.1:30837554-30837573 | None:intergenic | 55.0% | |
| AGATGATCCGGCATCAGCAG+TGG | + | chr5.1:30837423-30837442 | None:intergenic | 55.0% | |
| GAACAACTGGGTGGTCGAGT+TGG | + | chr5.1:30837340-30837359 | None:intergenic | 55.0% | |
| GATGCCGGATCATCTGGATC+TGG | - | chr5.1:30837428-30837447 | MS.gene77445:CDS | 55.0% | |
| GGCACAGGGAGTCTCCAAAT+TGG | + | chr5.1:30837540-30837559 | None:intergenic | 55.0% | |
| GGGAATGATCGGAAGGAAGG+TGG | + | chr5.1:30837385-30837404 | None:intergenic | 55.0% | |
| GGTCAACAGGAACAACTGGG+TGG | + | chr5.1:30837349-30837368 | None:intergenic | 55.0% | |
| TGCCTGTAGCGGTAGTCTCA+AGG | - | chr5.1:30837556-30837575 | MS.gene77445:CDS | 55.0% | |
| TGGGCAGGTTGTTGGTCAAC+AGG | + | chr5.1:30837362-30837381 | None:intergenic | 55.0% | |
| ! | GGTTGAGTGCCGTATCTGTC+AGG | - | chr5.1:30837499-30837518 | MS.gene77445:CDS | 55.0% |
| ! | TGAGTGCCGTATCTGTCAGG+AGG | - | chr5.1:30837502-30837521 | MS.gene77445:CDS | 55.0% |
| AGTGGCAGGAGATGGCGATG+GGG | + | chr5.1:30837405-30837424 | None:intergenic | 60.0% | |
| ATCGCCATCACGATCCACCG+CGG | + | chr5.1:30837456-30837475 | None:intergenic | 60.0% | |
| CAGTGGCAGGAGATGGCGAT+GGG | + | chr5.1:30837406-30837425 | None:intergenic | 60.0% | |
| GAGACTACCGCTACAGGCAC+AGG | + | chr5.1:30837555-30837574 | None:intergenic | 60.0% | |
| GAGACTCCCTGTGCCTGTAG+CGG | - | chr5.1:30837545-30837564 | MS.gene77445:CDS | 60.0% | |
| GCATCAGCAGTGGCAGGAGA+TGG | + | chr5.1:30837413-30837432 | None:intergenic | 60.0% | |
| GGAGGAGCCACTGATCCAGA+TGG | - | chr5.1:30837478-30837497 | MS.gene77445:CDS | 60.0% | |
| GGCGATGGGGAATGATCGGA+AGG | + | chr5.1:30837392-30837411 | None:intergenic | 60.0% | |
| TCTCCTGCCACTGCTGATGC+CGG | - | chr5.1:30837413-30837432 | MS.gene77445:CDS | 60.0% | |
| ! | ATCTGGATCTGGTTCCGCGG+TGG | - | chr5.1:30837439-30837458 | MS.gene77445:CDS | 60.0% |
| ! | GGATCGTGATGGCGATGAGG+AGG | - | chr5.1:30837460-30837479 | MS.gene77445:CDS | 60.0% |
| ! | GGTGGATCGTGATGGCGATG+AGG | - | chr5.1:30837457-30837476 | MS.gene77445:CDS | 60.0% |
| !! | GAAGGTGGTGGGCAGGTTGT+TGG | + | chr5.1:30837370-30837389 | None:intergenic | 60.0% |
| GATCCGGCATCAGCAGTGGC+AGG | + | chr5.1:30837419-30837438 | None:intergenic | 65.0% | |
| GCAGTGGCAGGAGATGGCGA+TGG | + | chr5.1:30837407-30837426 | None:intergenic | 65.0% | |
| GGTTCCGCGGTGGATCGTGA+TGG | - | chr5.1:30837449-30837468 | MS.gene77445:CDS | 65.0% | |
| !! | TCGGAAGGAAGGTGGTGGGC+AGG | + | chr5.1:30837377-30837396 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 30837308 | 30837598 | 30837308 | ID=MS.gene77445 |
| chr5.1 | mRNA | 30837308 | 30837598 | 30837308 | ID=MS.gene77445.t1;Parent=MS.gene77445 |
| chr5.1 | exon | 30837308 | 30837598 | 30837308 | ID=MS.gene77445.t1.exon1;Parent=MS.gene77445.t1 |
| chr5.1 | CDS | 30837308 | 30837598 | 30837308 | ID=cds.MS.gene77445.t1;Parent=MS.gene77445.t1 |
| Gene Sequence |
| Protein sequence |
