Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77576.t1 | XP_024636984.1 | 98.9 | 91 | 1 | 0 | 1 | 91 | 1 | 91 | 1.70E-43 | 184.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77576.t1 | I3T1S4 | 98.9 | 91 | 1 | 0 | 1 | 91 | 1 | 91 | 1.2e-43 | 184.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050556 | MS.gene77576 | -0.809973 | 1.45E-50 | -1.69E-46 |
| MS.gene051631 | MS.gene77576 | 0.806157 | 9.43E-50 | -1.69E-46 |
| MS.gene052242 | MS.gene77576 | 0.820801 | 5.66E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77576.t1 | MTR_4g078485 | 98.780 | 82 | 1 | 0 | 1 | 82 | 51 | 132 | 4.04e-53 | 164 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77576.t1 | AT1G65032 | 61.905 | 84 | 32 | 0 | 3 | 86 | 2 | 85 | 2.12e-33 | 110 |
Find 18 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAGGTACGTGCAATGTTT+TGG | 0.338129 | 3.2:-73076346 | MS.gene77576:CDS |
| GAATGGGAAAGGGTTTGATA+TGG | 0.351995 | 3.2:-73076490 | MS.gene77576:CDS |
| AGTATTCAGCAGCATCAATT+AGG | 0.357297 | 3.2:+73076285 | None:intergenic |
| CAGAGGATCTGCATAGGAAC+AGG | 0.475737 | 3.2:-73076460 | MS.gene77576:CDS |
| CTTCGCTAGTCTTGCAGCGA+AGG | 0.478795 | 3.2:+73076373 | None:intergenic |
| GGTGTGTTGCAGAATGGGAA+AGG | 0.507863 | 3.2:-73076501 | None:intergenic |
| AATGGGAAAGGGTTTGATAT+GGG | 0.518936 | 3.2:-73076489 | MS.gene77576:CDS |
| TTTGATATGGGCGACGGCAG+AGG | 0.527398 | 3.2:-73076477 | MS.gene77576:CDS |
| GTGTGTTGCAGAATGGGAAA+GGG | 0.536633 | 3.2:-73076500 | None:intergenic |
| TTTGGGTGGGTTCTGATGAA+CGG | 0.599950 | 3.2:-73076328 | MS.gene77576:CDS |
| GCGAAGGTCAGTGGCAACGA+AGG | 0.601082 | 3.2:+73076389 | None:intergenic |
| AGTCTTGCAGCGAAGGTCAG+TGG | 0.626755 | 3.2:+73076380 | None:intergenic |
| CGACGGCAGAGGATCTGCAT+AGG | 0.631254 | 3.2:-73076466 | MS.gene77576:CDS |
| AAAGGGTTTGATATGGGCGA+CGG | 0.633143 | 3.2:-73076483 | MS.gene77576:CDS |
| GTGGCAACGAAGGAGAATTG+AGG | 0.641838 | 3.2:+73076399 | None:intergenic |
| AAGACTAGCGAAGAAAGCTG+AGG | 0.705121 | 3.2:-73076363 | MS.gene77576:CDS |
| GATCAGGTAATGTGACGAGG+AGG | 0.706235 | 3.2:+73076233 | None:intergenic |
| GTTGATCAGGTAATGTGACG+AGG | 0.712528 | 3.2:+73076230 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTCTATCTTTTCTCAAGAA+AGG | - | chr3.2:73076460-73076479 | MS.gene77576:CDS | 30.0% |
| AGTATTCAGCAGCATCAATT+AGG | + | chr3.2:73076441-73076460 | None:intergenic | 35.0% | |
| ! | TGAGGTACGTGCAATGTTTT+GGG | - | chr3.2:73076378-73076397 | MS.gene77576:CDS | 40.0% |
| AAAGGGTTTGATATGGGCGA+CGG | - | chr3.2:73076240-73076259 | MS.gene77576:CDS | 45.0% | |
| AAGACTAGCGAAGAAAGCTG+AGG | - | chr3.2:73076360-73076379 | MS.gene77576:CDS | 45.0% | |
| ! | CTGAGGTACGTGCAATGTTT+TGG | - | chr3.2:73076377-73076396 | MS.gene77576:CDS | 45.0% |
| ! | TTTGGGTGGGTTCTGATGAA+CGG | - | chr3.2:73076395-73076414 | MS.gene77576:CDS | 45.0% |
| !! | GTACGTGCAATGTTTTGGGT+GGG | - | chr3.2:73076382-73076401 | MS.gene77576:CDS | 45.0% |
| CAGAGGATCTGCATAGGAAC+AGG | - | chr3.2:73076263-73076282 | MS.gene77576:CDS | 50.0% | |
| GTGGCAACGAAGGAGAATTG+AGG | + | chr3.2:73076327-73076346 | None:intergenic | 50.0% | |
| !! | GGTACGTGCAATGTTTTGGG+TGG | - | chr3.2:73076381-73076400 | MS.gene77576:CDS | 50.0% |
| AGTCTTGCAGCGAAGGTCAG+TGG | + | chr3.2:73076346-73076365 | None:intergenic | 55.0% | |
| TTTGATATGGGCGACGGCAG+AGG | - | chr3.2:73076246-73076265 | MS.gene77576:CDS | 55.0% | |
| !! | CTTCGCTAGTCTTGCAGCGA+AGG | + | chr3.2:73076353-73076372 | None:intergenic | 55.0% |
| CGACGGCAGAGGATCTGCAT+AGG | - | chr3.2:73076257-73076276 | MS.gene77576:CDS | 60.0% | |
| GCGAAGGTCAGTGGCAACGA+AGG | + | chr3.2:73076337-73076356 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 73076235 | 73076510 | 73076235 | ID=MS.gene77576 |
| chr3.2 | mRNA | 73076235 | 73076510 | 73076235 | ID=MS.gene77576.t1;Parent=MS.gene77576 |
| chr3.2 | exon | 73076235 | 73076510 | 73076235 | ID=MS.gene77576.t1.exon1;Parent=MS.gene77576.t1 |
| chr3.2 | CDS | 73076235 | 73076510 | 73076235 | ID=cds.MS.gene77576.t1;Parent=MS.gene77576.t1 |
| Gene Sequence |
| Protein sequence |
