AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78368

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78368.t1 MTR_2g098310 94.118 425 12 3 1 425 1 412 0.0 811
MS.gene78368.t1 MTR_2g098310 86.957 460 12 4 1 425 1 447 0.0 774
MS.gene78368.t1 MTR_2g098310 92.892 408 16 3 1 408 1 395 0.0 750
MS.gene78368.t1 MTR_2g098310 93.473 383 12 3 1 383 1 370 0.0 703
MS.gene78368.t1 MTR_2g098310 93.094 362 12 3 1 362 1 349 0.0 655
MS.gene78368.t1 MTR_4g065870 51.288 466 151 16 1 423 1 433 2.39e-144 418
MS.gene78368.t1 MTR_4g065870 50.557 449 146 16 1 406 1 416 2.41e-134 392
MS.gene78368.t1 MTR_4g065870 49.061 426 141 16 1 383 1 393 4.44e-119 353
MS.gene78368.t1 MTR_1g052470 46.809 329 140 9 114 421 60 374 6.32e-77 244
MS.gene78368.t1 MTR_3g028520 36.427 431 216 14 3 423 18 400 7.54e-58 195
MS.gene78368.t1 MTR_3g027650 36.427 431 216 14 3 423 18 400 7.54e-58 195
MS.gene78368.t1 MTR_3g027650 36.473 414 205 14 3 406 18 383 1.13e-51 179
MS.gene78368.t1 MTR_7g092540 43.529 255 119 5 170 421 87 319 7.46e-48 166
MS.gene78368.t1 MTR_8g036235 35.252 417 203 13 8 417 32 388 2.73e-47 167
MS.gene78368.t1 MTR_7g092540 44.215 242 110 5 170 408 87 306 2.93e-44 156
MS.gene78368.t1 MTR_1g052470 40.511 274 120 9 114 362 60 315 8.83e-42 150
MS.gene78368.t1 MTR_3g027650 33.760 391 201 14 3 383 18 360 5.14e-37 139
MS.gene78368.t1 MTR_1g116790 61.404 114 39 2 310 421 231 341 8.16e-36 135
MS.gene78368.t1 MTR_4g033295 70.115 87 24 1 331 417 106 190 4.49e-34 126
MS.gene78368.t1 MTR_8g036235 32.323 396 197 14 8 396 32 363 1.17e-30 122
MS.gene78368.t1 MTR_4g033295 75.000 76 17 1 331 406 106 179 1.64e-30 116
MS.gene78368.t1 MTR_6g052280 62.319 69 26 0 315 383 24 92 3.94e-21 89.0
MS.gene78368.t1 MTR_4g033295 79.245 53 9 1 331 383 106 156 4.29e-20 87.8
MS.gene78368.t1 MTR_4g131160 58.462 65 26 1 344 408 195 258 5.97e-16 79.7
MS.gene78368.t1 MTR_4g131160 58.462 65 26 1 344 408 198 261 6.13e-16 79.7
MS.gene78368.t1 MTR_1g017350 47.674 86 37 1 323 408 246 323 7.54e-16 79.3
MS.gene78368.t1 MTR_1g017350 47.674 86 37 1 323 408 246 323 8.23e-16 79.3
MS.gene78368.t1 MTR_1g017350 47.674 86 37 1 323 408 246 323 8.98e-16 79.3
MS.gene78368.t1 MTR_1g017350 47.674 86 37 1 323 408 246 323 9.10e-16 79.3
MS.gene78368.t1 MTR_8g012290 51.282 78 31 1 338 408 277 354 3.66e-15 77.8
MS.gene78368.t1 MTR_8g012290 51.282 78 31 1 338 408 277 354 3.70e-15 77.8
MS.gene78368.t1 MTR_3g116770 56.061 66 29 0 343 408 189 254 3.81e-15 77.0
MS.gene78368.t1 MTR_3g116770 56.061 66 29 0 343 408 189 254 4.15e-15 77.0
MS.gene78368.t1 MTR_7g053410 37.097 124 69 2 286 408 265 380 4.80e-15 77.4
MS.gene78368.t1 MTR_7g053410 37.097 124 69 2 286 408 265 380 4.98e-15 77.4
MS.gene78368.t1 MTR_3g498825 56.061 66 29 0 343 408 145 210 5.47e-15 75.9
MS.gene78368.t1 MTR_1g080890 55.385 65 28 1 344 408 192 255 1.58e-14 75.1
MS.gene78368.t1 MTR_8g062240 33.333 177 101 5 235 408 52 214 1.79e-14 73.9
MS.gene78368.t1 MTR_8g065740 56.250 64 28 0 345 408 305 368 2.44e-14 75.1
MS.gene78368.t1 MTR_4g070320 53.030 66 31 0 343 408 141 206 3.01e-14 73.9
MS.gene78368.t1 MTR_5g048860 48.052 77 32 1 332 408 360 428 4.05e-14 74.7
MS.gene78368.t1 MTR_8g099880 51.515 66 32 0 343 408 143 208 2.42e-13 71.2
MS.gene78368.t1 MTR_5g037250 50.000 66 33 0 343 408 110 175 1.37e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78368.t1 AT1G51140 37.825 423 195 13 5 417 12 376 5.71e-68 221
MS.gene78368.t1 AT2G42280 37.063 429 173 14 11 423 10 357 7.60e-66 214
MS.gene78368.t1 AT2G42280 37.073 410 161 14 11 404 10 338 1.68e-60 201
MS.gene78368.t1 AT1G35460 68.966 87 27 0 337 423 173 259 3.95e-37 136
MS.gene78368.t1 AT4G09180 62.136 103 39 0 321 423 160 262 4.35e-37 136
MS.gene78368.t1 AT2G43140 61.538 104 39 1 315 417 103 206 3.42e-36 132
MS.gene78368.t1 AT2G43140 38.281 256 109 10 177 417 82 303 1.66e-34 130
MS.gene78368.t1 AT2G42280 30.978 368 157 14 11 362 10 296 6.72e-31 120
MS.gene78368.t1 AT1G35460 80.851 47 9 0 337 383 173 219 9.71e-21 90.9
MS.gene78368.t1 AT2G24260 60.000 65 25 1 344 408 34 97 1.15e-16 79.7
MS.gene78368.t1 AT2G24260 60.000 65 25 1 344 408 137 200 3.26e-16 80.1
MS.gene78368.t1 AT4G34530 50.000 86 41 1 323 408 152 235 5.26e-16 79.3
MS.gene78368.t1 AT4G30980 58.462 65 26 1 344 408 129 192 6.88e-16 78.6
MS.gene78368.t1 AT4G30980 58.462 65 26 1 344 408 177 240 1.06e-15 78.6
MS.gene78368.t1 AT5G50915 56.061 66 29 0 343 408 134 199 1.55e-15 76.6
MS.gene78368.t1 AT5G50915 56.061 66 29 0 343 408 134 199 1.55e-15 76.6
MS.gene78368.t1 AT5G50915 56.061 66 29 0 343 408 134 199 1.74e-15 77.0
MS.gene78368.t1 AT5G50915 56.061 66 29 0 343 408 134 199 1.74e-15 77.0
MS.gene78368.t1 AT1G10120 49.398 83 34 1 326 408 195 269 3.39e-15 77.0
MS.gene78368.t1 AT1G10120 49.398 83 34 1 326 408 195 269 3.39e-15 77.0
MS.gene78368.t1 AT1G68920 54.545 66 30 0 343 408 300 365 5.23e-15 77.4
MS.gene78368.t1 AT1G68920 54.545 66 30 0 343 408 301 366 5.39e-15 77.0
MS.gene78368.t1 AT1G68920 54.545 66 30 0 343 408 301 366 5.39e-15 77.0
MS.gene78368.t1 AT1G68920 54.545 66 30 0 343 408 301 366 5.39e-15 77.0
MS.gene78368.t1 AT5G58010 53.846 65 29 1 344 408 98 161 6.01e-15 75.5
MS.gene78368.t1 AT3G07340 54.545 66 30 0 343 408 256 321 8.43e-15 76.3
MS.gene78368.t1 AT3G07340 54.545 66 30 0 343 408 256 321 8.70e-15 76.3
MS.gene78368.t1 AT1G25330 53.030 66 31 0 343 408 102 167 8.73e-15 73.6
MS.gene78368.t1 AT1G26260 56.061 66 29 0 343 408 199 264 1.77e-14 75.1
MS.gene78368.t1 AT1G26260 56.061 66 29 0 343 408 221 286 2.02e-14 75.1
MS.gene78368.t1 AT1G26260 56.061 66 29 0 343 408 221 286 2.02e-14 75.1
MS.gene78368.t1 AT1G26260 56.061 66 29 0 343 408 221 286 2.02e-14 75.1
MS.gene78368.t1 AT1G26260 56.061 66 29 0 343 408 221 286 2.02e-14 75.1
MS.gene78368.t1 AT1G26260 56.061 66 29 0 343 408 170 235 2.39e-14 74.3
MS.gene78368.t1 AT5G48560 53.030 66 31 0 343 408 299 364 2.72e-14 75.1
MS.gene78368.t1 AT5G48560 53.030 66 31 0 343 408 299 364 3.00e-14 74.7
MS.gene78368.t1 AT3G23690 51.515 66 32 0 343 408 189 254 1.66e-13 72.0
MS.gene78368.t1 AT1G59640 51.515 66 32 0 343 408 134 199 1.92e-13 70.5
MS.gene78368.t1 AT1G18400 50.000 66 33 0 343 408 143 208 2.19e-13 70.1
MS.gene78368.t1 AT1G73830 51.562 64 31 0 345 408 147 210 2.21e-13 70.5
MS.gene78368.t1 AT1G73830 51.562 64 31 0 345 408 147 210 2.26e-13 70.5
MS.gene78368.t1 AT1G18400 51.562 64 31 0 345 408 145 208 4.38e-13 69.3
MS.gene78368.t1 AT2G18300 50.000 64 32 0 345 408 185 248 6.77e-13 69.7
MS.gene78368.t1 AT1G59640 53.125 64 30 0 345 408 136 199 6.99e-13 69.7
MS.gene78368.t1 AT2G18300 50.000 64 32 0 345 408 185 248 7.58e-13 69.7
MS.gene78368.t1 AT2G18300 50.000 64 32 0 345 408 185 248 1.00e-12 69.7
MS.gene78368.t1 AT4G36540 48.485 66 34 0 343 408 139 204 1.43e-12 68.6
MS.gene78368.t1 AT4G36540 48.485 66 34 0 343 408 139 204 1.54e-12 68.6
MS.gene78368.t1 AT2G42300 47.222 72 37 1 338 408 177 248 2.31e-12 68.2
MS.gene78368.t1 AT2G42300 47.222 72 37 1 338 408 177 248 3.13e-12 67.0
MS.gene78368.t1 AT5G62610 46.970 66 35 0 343 408 151 216 9.70e-12 65.9
MS.gene78368.t1 AT5G62610 46.970 66 35 0 343 408 151 216 9.70e-12 65.9
MS.gene78368.t1 AT3G57800 46.970 66 35 0 343 408 202 267 1.03e-11 66.6

Find 69 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCATTTCAGATGAAGTATT+TGG 0.195078 2.3:+5056550 None:intergenic
TTCCTTGAAGTAGAAAATTT+TGG 0.197445 2.3:+5056958 None:intergenic
TCTCAAATTAACATTGAAAA+TGG 0.226358 2.3:-5057713 MS.gene78368:intron
ACCCTTTCGGCAATGCTTCT+TGG 0.230259 2.3:+5056451 None:intergenic
CCAAATCAGAATGTTTCTTC+TGG 0.278715 2.3:-5057845 MS.gene78368:CDS
TCAAGGTTTATTAACAGTTT+TGG 0.288729 2.3:-5058115 MS.gene78368:CDS
CCCTTTCGGCAATGCTTCTT+GGG 0.290663 2.3:+5056452 None:intergenic
CATGGTACCTTATCCATGTT+TGG 0.320678 2.3:+5056027 None:intergenic
TAAATTGGCTTCAGGTTAGA+AGG 0.325551 2.3:-5056092 MS.gene78368:intron
CACCCACAAGTAAATTCTTC+AGG 0.335781 2.3:-5058250 MS.gene78368:CDS
CCAGAAGAAACATTCTGATT+TGG 0.339425 2.3:+5057845 None:intergenic
ATGGTTGATTAGAATGAAAC+AGG 0.344190 2.3:-5056616 MS.gene78368:intron
ATCAATAATCCATACCCTTT+CGG 0.345815 2.3:+5056438 None:intergenic
AATGAAGAAAATGAAGCTTT+TGG 0.348594 2.3:-5056856 MS.gene78368:CDS
ATGAGAGGAATGGGAACTTT+AGG 0.362085 2.3:-5057006 MS.gene78368:CDS
GCCAAATACTTCATCTGAAA+TGG 0.370699 2.3:-5056551 MS.gene78368:CDS
AAGTCATGGTAACGAATATA+TGG 0.373522 2.3:-5056830 MS.gene78368:CDS
AATGTTTCTTCTGGTTTAGA+TGG 0.373647 2.3:-5057836 MS.gene78368:CDS
AGGACATTCTAATTCTCCTT+TGG 0.389735 2.3:-5056590 MS.gene78368:CDS
CCCAAGAAGCATTGCCGAAA+GGG 0.390620 2.3:-5056452 MS.gene78368:intron
AATCAATCAAACTATTCATC+AGG 0.391137 2.3:-5056928 MS.gene78368:CDS
TAACGAATATATGGATTATC+AGG 0.397601 2.3:-5056821 MS.gene78368:CDS
AACTCTGTTCCTTCTTCTCA+TGG 0.404649 2.3:-5057899 MS.gene78368:CDS
TTCGGCAATGCTTCTTGGGT+GGG 0.408101 2.3:+5056456 None:intergenic
CGCTCTGTTTCTGGACTCGA+TGG 0.418065 2.3:+5058145 None:intergenic
TGGATTATCAGGTTGATACT+TGG 0.436048 2.3:-5056810 MS.gene78368:CDS
TTCATCTGAAATGGCAGCTA+TGG 0.443352 2.3:-5056542 MS.gene78368:CDS
CTAAAATTAGTGAGCGTATG+AGG 0.443405 2.3:-5056068 MS.gene78368:CDS
AGCAGCAACAACAACAATAA+CGG 0.452797 2.3:-5055693 None:intergenic
ATGGTACCTTATCCATGTTT+GGG 0.462347 2.3:+5056028 None:intergenic
TATGTTAGTATGAGAGGAAT+GGG 0.466243 2.3:-5057015 MS.gene78368:CDS
TATGAGGAAGCTACAAGATC+TGG 0.475457 2.3:-5056052 MS.gene78368:CDS
TTTCGGCAATGCTTCTTGGG+TGG 0.478638 2.3:+5056455 None:intergenic
TTTCCACAATGATTCTTCAC+TGG 0.486339 2.3:+5057782 None:intergenic
AACCTGAAGAATTTACTTGT+GGG 0.489854 2.3:+5058248 None:intergenic
TCTGGTCCCAAACATGGATA+AGG 0.497918 2.3:-5056034 MS.gene78368:intron
GTTGTTGTTGCTGCTTGTGT+GGG 0.500762 2.3:+5055701 None:intergenic
ACAACAACAAGATCAGGGTT+TGG 0.506743 2.3:-5057996 MS.gene78368:CDS
GTGGGTGGCACAACCTCGCT+TGG 0.508441 2.3:+5056474 None:intergenic
GGCAATGCTTCTTGGGTGGG+TGG 0.511007 2.3:+5056459 None:intergenic
TGTTGTTGTTGCTGCTTGTG+TGG 0.515976 2.3:+5055700 None:intergenic
CACAGCAGATATGTTGGACT+TGG 0.518004 2.3:-5055899 MS.gene78368:CDS
AACAGTGAAAGAAGAAGAAG+AGG 0.518044 2.3:-5058047 MS.gene78368:CDS
GCAGGTTATGTTAGTATGAG+AGG 0.524043 2.3:-5057021 MS.gene78368:intron
ATTTGAGAAAATAATCCAGC+TGG 0.524222 2.3:+5057728 None:intergenic
GGATTATCAGGTTGATACTT+GGG 0.529420 2.3:-5056809 MS.gene78368:CDS
AACAAACACAGCAGATATGT+TGG 0.533839 2.3:-5055905 MS.gene78368:CDS
ATGAAAATTCGTGCCAAGCG+AGG 0.540193 2.3:-5056487 MS.gene78368:CDS
ACATTCTGATTTGGCAAAGG+TGG 0.545087 2.3:+5057854 None:intergenic
AGATATGTTGGACTTGGCTG+TGG 0.548724 2.3:-5055893 MS.gene78368:CDS
AGACCTTCAAAAGCAAGCTC+AGG 0.554514 2.3:-5055860 MS.gene78368:intron
GAAACATTCTGATTTGGCAA+AGG 0.556566 2.3:+5057851 None:intergenic
TTATGTTAGTATGAGAGGAA+TGG 0.558938 2.3:-5057016 MS.gene78368:CDS
AGACATAGTAGTTCACCAGC+TGG 0.569208 2.3:-5057743 MS.gene78368:CDS
ATGTCAACAATAGCTGAAGT+TGG 0.572331 2.3:-5056901 MS.gene78368:CDS
ACAAGATCTGGTCCCAAACA+TGG 0.576424 2.3:-5056040 MS.gene78368:CDS
ATCAATCAAACTATTCATCA+GGG 0.580340 2.3:-5056927 MS.gene78368:CDS
ACCCAAGAAGCATTGCCGAA+AGG 0.582792 2.3:-5056453 MS.gene78368:CDS
AAACCTGAAGAATTTACTTG+TGG 0.586193 2.3:+5058247 None:intergenic
ATAGCTGAAGTTGGTGACAA+AGG 0.592602 2.3:-5056892 MS.gene78368:CDS
TAAATTCTTCAGGTTTAACA+AGG 0.602353 2.3:-5058240 MS.gene78368:CDS
TTCAATCAACAATGAACATG+TGG 0.608573 2.3:-5057972 MS.gene78368:CDS
GATTAGAATGAAACAGGAGG+AGG 0.610884 2.3:-5056610 MS.gene78368:intron
ATGATGTCTGAAAATGTTGG+TGG 0.620048 2.3:-5056775 MS.gene78368:CDS
ATACTCAATTGATGAGCCAA+AGG 0.626112 2.3:+5056574 None:intergenic
CAGCCAGTGAAGAATCATTG+TGG 0.646576 2.3:-5057785 MS.gene78368:CDS
ATGTTGTTGAAATATGAACT+TGG 0.650570 2.3:+5058205 None:intergenic
GAAATGATGTCTGAAAATGT+TGG 0.668717 2.3:-5056778 MS.gene78368:CDS
GTTGATTAGAATGAAACAGG+AGG 0.674543 2.3:-5056613 MS.gene78368:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTAGTTATTACTTATT+AGG - chr2.3:5056854-5056873 MS.gene78368:CDS 10.0%
!! TTAAAGAAATAAAATAGTTT+GGG + chr2.3:5056893-5056912 None:intergenic 10.0%
!! AAACAAAAGAAAAGTAATAT+AGG + chr2.3:5057722-5057741 None:intergenic 15.0%
!! AACAAAAGAAAAGTAATATA+GGG + chr2.3:5057721-5057740 None:intergenic 15.0%
!! ATATATTAAAGATCTCTTTA+GGG - chr2.3:5056735-5056754 MS.gene78368:CDS 15.0%
!! CTAAAACTAAATATGTAAAT+TGG + chr2.3:5057758-5057777 None:intergenic 15.0%
!! GTTAAAGAAATAAAATAGTT+TGG + chr2.3:5056894-5056913 None:intergenic 15.0%
!! TAAAGAAATAAAATAGTTTG+GGG + chr2.3:5056892-5056911 None:intergenic 15.0%
!! TATATATTAAAGATCTCTTT+AGG - chr2.3:5056734-5056753 MS.gene78368:CDS 15.0%
!!! ATATTACTTTTCTTTTGTTT+AGG - chr2.3:5057722-5057741 MS.gene78368:CDS 15.0%
!!! TATTTTTTTCTCTTTAAAGT+AGG + chr2.3:5056651-5056670 None:intergenic 15.0%
!!! TTCAAATTTAACATTTTTCT+AGG + chr2.3:5056681-5056700 None:intergenic 15.0%
!!! TTTTAATATTTGCTATTATG+TGG - chr2.3:5056769-5056788 MS.gene78368:CDS 15.0%
!! CATTCACATAAATTAATGTA+TGG + chr2.3:5058008-5058027 None:intergenic 20.0%
!! TCTCAAATTAACATTGAAAA+TGG - chr2.3:5056260-5056279 MS.gene78368:intron 20.0%
!! TGAAGAAAATGAATTGTAAA+AGG - chr2.3:5056521-5056540 MS.gene78368:CDS 20.0%
!!! AATTTGAAAATTTTCTTGTC+AGG - chr2.3:5056693-5056712 MS.gene78368:intron 20.0%
!!! ACTATTAAGACATGATTTTT+GGG - chr2.3:5057784-5057803 MS.gene78368:CDS 20.0%
!!! ATTTGAAAATTTTCTTGTCA+GGG - chr2.3:5056694-5056713 MS.gene78368:intron 20.0%
!!! TACTATTAAGACATGATTTT+TGG - chr2.3:5057783-5057802 MS.gene78368:CDS 20.0%
! AATAACTAATATTCCCACTA+GGG + chr2.3:5056847-5056866 None:intergenic 25.0%
! AATCAATCAAACTATTCATC+AGG - chr2.3:5057045-5057064 MS.gene78368:intron 25.0%
! ATCAATCAAACTATTCATCA+GGG - chr2.3:5057046-5057065 MS.gene78368:intron 25.0%
! ATGTTGTTGAAATATGAACT+TGG + chr2.3:5055771-5055790 None:intergenic 25.0%
! CTCGTTTCTATTAAAATAGA+GGG + chr2.3:5056335-5056354 None:intergenic 25.0%
! CTCTTAACACATCATTAAAT+TGG - chr2.3:5057866-5057885 MS.gene78368:CDS 25.0%
! GTGTTGGAAGAAAATTAATT+AGG + chr2.3:5058212-5058231 None:intergenic 25.0%
! TAATAACTAATATTCCCACT+AGG + chr2.3:5056848-5056867 None:intergenic 25.0%
! TAGAATTATCTATAACCAAG+TGG + chr2.3:5057684-5057703 None:intergenic 25.0%
! TCTAATAAAATCCACCATTT+CGG + chr2.3:5056473-5056492 None:intergenic 25.0%
! TCTCGTTTCTATTAAAATAG+AGG + chr2.3:5056336-5056355 None:intergenic 25.0%
! TGAAAAAAAAAAACCCTAGT+GGG - chr2.3:5056831-5056850 MS.gene78368:CDS 25.0%
! TGATGTTATGCAAGTTTAAT+TGG - chr2.3:5056930-5056949 MS.gene78368:CDS 25.0%
! TGTGACTAAAATTTCAGATT+TGG + chr2.3:5056373-5056392 None:intergenic 25.0%
!! AACCAAAATTTTCTACTTCA+AGG - chr2.3:5057013-5057032 MS.gene78368:CDS 25.0%
!! AATGAAGAAAATGAAGCTTT+TGG - chr2.3:5057117-5057136 MS.gene78368:intron 25.0%
!! ATGAATTGTAAAAGGTTTTC+TGG - chr2.3:5056529-5056548 MS.gene78368:CDS 25.0%
!! CATTTGACATGATTTTGTTA+TGG - chr2.3:5057338-5057357 MS.gene78368:intron 25.0%
!! CTATTAAGACATGATTTTTG+GGG - chr2.3:5057785-5057804 MS.gene78368:CDS 25.0%
!! GATTCAAAACAACAATTTTC+TGG - chr2.3:5057243-5057262 MS.gene78368:intron 25.0%
!! TAAATTCTTCAGGTTTAACA+AGG - chr2.3:5055733-5055752 MS.gene78368:CDS 25.0%
!! TAACGAATATATGGATTATC+AGG - chr2.3:5057152-5057171 MS.gene78368:intron 25.0%
!! TCAAGGTTTATTAACAGTTT+TGG - chr2.3:5055858-5055877 MS.gene78368:intron 25.0%
!! TTCCTTGAAGTAGAAAATTT+TGG + chr2.3:5057018-5057037 None:intergenic 25.0%
!! TTTGAAAATTTTCTTGTCAG+GGG - chr2.3:5056695-5056714 MS.gene78368:intron 25.0%
!!! ATTTTCTGGTTTAAATGTTC+AGG - chr2.3:5057257-5057276 MS.gene78368:intron 25.0%
!!! TTAGATGGAAGTTTTTCAAT+GGG - chr2.3:5056152-5056171 MS.gene78368:intron 25.0%
!!! TTTAGATGGAAGTTTTTCAA+TGG - chr2.3:5056151-5056170 MS.gene78368:intron 25.0%
!!! TTTTTACCTTAGTTCTTATG+AGG - chr2.3:5057568-5057587 MS.gene78368:intron 25.0%
AAACCTGAAGAATTTACTTG+TGG + chr2.3:5055729-5055748 None:intergenic 30.0%
AACCTGAAGAATTTACTTGT+GGG + chr2.3:5055728-5055747 None:intergenic 30.0%
ATCAATAATCCATACCCTTT+CGG + chr2.3:5057538-5057557 None:intergenic 30.0%
ATGGTTGATTAGAATGAAAC+AGG - chr2.3:5057357-5057376 MS.gene78368:intron 30.0%
ATTTGAGAAAATAATCCAGC+TGG + chr2.3:5056248-5056267 None:intergenic 30.0%
CTCAAATGGTTAATAAGCTT+CGG + chr2.3:5057657-5057676 None:intergenic 30.0%
GAAATGATGTCTGAAAATGT+TGG - chr2.3:5057195-5057214 MS.gene78368:intron 30.0%
GAACCTCAATGTACATATAA+TGG + chr2.3:5057811-5057830 None:intergenic 30.0%
GTGAAAAAAAAAAACCCTAG+TGG - chr2.3:5056830-5056849 MS.gene78368:CDS 30.0%
GTGTTTGTTTGCTTCAAAAA+AGG + chr2.3:5058057-5058076 None:intergenic 30.0%
TATGTTAGTATGAGAGGAAT+GGG - chr2.3:5056958-5056977 MS.gene78368:CDS 30.0%
TCGTAAGTGTAGTTTCTTAA+TGG - chr2.3:5057310-5057329 MS.gene78368:intron 30.0%
TGATAAAAACCATGAGAAGA+AGG + chr2.3:5056086-5056105 None:intergenic 30.0%
TTAAAACCAATCATGAAGCA+TGG + chr2.3:5057967-5057986 None:intergenic 30.0%
TTATGTTAGTATGAGAGGAA+TGG - chr2.3:5056957-5056976 MS.gene78368:CDS 30.0%
TTCAATCAACAATGAACATG+TGG - chr2.3:5056001-5056020 MS.gene78368:intron 30.0%
! AAGTCATGGTAACGAATATA+TGG - chr2.3:5057143-5057162 MS.gene78368:intron 30.0%
! TTTTCTTTGTTACGCACTTT+TGG - chr2.3:5056308-5056327 MS.gene78368:intron 30.0%
!! AATGTTTCTTCTGGTTTAGA+TGG - chr2.3:5056137-5056156 MS.gene78368:intron 30.0%
!! GTTTTTCAATGGGAGTTAAT+AGG - chr2.3:5056162-5056181 MS.gene78368:intron 30.0%
!! TTTCTCTTTAAAGTAGGACA+AGG + chr2.3:5056645-5056664 None:intergenic 30.0%
!!! TACTGATTTTTGATCCGAAA+TGG - chr2.3:5056456-5056475 MS.gene78368:CDS 30.0%
AAAGTAGGACAAGGAAAACT+GGG + chr2.3:5056636-5056655 None:intergenic 35.0%
AACAAACACAGCAGATATGT+TGG - chr2.3:5058068-5058087 MS.gene78368:CDS 35.0%
AACAGTGAAAGAAGAAGAAG+AGG - chr2.3:5055926-5055945 MS.gene78368:intron 35.0%
ACAACAACAACAACAAGATC+AGG - chr2.3:5055971-5055990 MS.gene78368:intron 35.0%
AGGACATTCTAATTCTCCTT+TGG - chr2.3:5057383-5057402 MS.gene78368:intron 35.0%
ATACTCAATTGATGAGCCAA+AGG + chr2.3:5057402-5057421 None:intergenic 35.0%
ATGATGTCTGAAAATGTTGG+TGG - chr2.3:5057198-5057217 MS.gene78368:intron 35.0%
ATGGTACCTTATCCATGTTT+GGG + chr2.3:5057948-5057967 None:intergenic 35.0%
ATGTCAACAATAGCTGAAGT+TGG - chr2.3:5057072-5057091 MS.gene78368:intron 35.0%
CAACAACAACAACAAGATCA+GGG - chr2.3:5055972-5055991 MS.gene78368:intron 35.0%
CCAAATCAGAATGTTTCTTC+TGG - chr2.3:5056128-5056147 MS.gene78368:intron 35.0%
CCAGAAGAAACATTCTGATT+TGG + chr2.3:5056131-5056150 None:intergenic 35.0%
CTAAAATTAGTGAGCGTATG+AGG - chr2.3:5057905-5057924 MS.gene78368:CDS 35.0%
GCCAAATACTTCATCTGAAA+TGG - chr2.3:5057422-5057441 MS.gene78368:intron 35.0%
GGTAGAATACTATCTAGCAT+TGG - chr2.3:5057278-5057297 MS.gene78368:intron 35.0%
GTTATGCAAGTTTAATTGGC+AGG - chr2.3:5056934-5056953 MS.gene78368:CDS 35.0%
GTTGATTAGAATGAAACAGG+AGG - chr2.3:5057360-5057379 MS.gene78368:intron 35.0%
TAAAGTAGGACAAGGAAAAC+TGG + chr2.3:5056637-5056656 None:intergenic 35.0%
TACTAGTATGTGGACTTGTA+AGG - chr2.3:5056569-5056588 MS.gene78368:CDS 35.0%
TACTTGCCTCATAAGAACTA+AGG + chr2.3:5057577-5057596 None:intergenic 35.0%
TAGTACACGTTACTAGTATG+TGG - chr2.3:5056559-5056578 MS.gene78368:CDS 35.0%
TATTGAAGTTGTACATGCCA+CGG + chr2.3:5056601-5056620 None:intergenic 35.0%
TGAAGCTTCTATACAAGTGT+TGG + chr2.3:5058228-5058247 None:intergenic 35.0%
TGGTTAATAAGCTTCGGTTA+AGG + chr2.3:5057651-5057670 None:intergenic 35.0%
TTTCCACAATGATTCTTCAC+TGG + chr2.3:5056194-5056213 None:intergenic 35.0%
! CACATCATTAAATTGGCTTC+AGG - chr2.3:5057873-5057892 MS.gene78368:CDS 35.0%
! GCCATTTCAGATGAAGTATT+TGG + chr2.3:5057426-5057445 None:intergenic 35.0%
! TAAATTGGCTTCAGGTTAGA+AGG - chr2.3:5057881-5057900 MS.gene78368:CDS 35.0%
! TGATTTTTGATCCGAAATGG+TGG - chr2.3:5056459-5056478 MS.gene78368:CDS 35.0%
! TTTGTACTTGTTGCAACAAG+TGG - chr2.3:5056808-5056827 MS.gene78368:CDS 35.0%
!! GAAACATTCTGATTTGGCAA+AGG + chr2.3:5056125-5056144 None:intergenic 35.0%
!! GGATTATCAGGTTGATACTT+GGG - chr2.3:5057164-5057183 MS.gene78368:intron 35.0%
!! TGGATTATCAGGTTGATACT+TGG - chr2.3:5057163-5057182 MS.gene78368:intron 35.0%
!!! AAACAGAGCGAGTTTTTTCA+AGG - chr2.3:5055841-5055860 MS.gene78368:intron 35.0%
AACCATTTGAGCAAACCACT+TGG - chr2.3:5057666-5057685 MS.gene78368:intron 40.0%
AACTCTGTTCCTTCTTCTCA+TGG - chr2.3:5056074-5056093 MS.gene78368:CDS 40.0%
ACAACAACAAGATCAGGGTT+TGG - chr2.3:5055977-5055996 MS.gene78368:intron 40.0%
ATGAGAGGAATGGGAACTTT+AGG - chr2.3:5056967-5056986 MS.gene78368:CDS 40.0%
CACCCACAAGTAAATTCTTC+AGG - chr2.3:5055723-5055742 MS.gene78368:CDS 40.0%
CATGGTACCTTATCCATGTT+TGG + chr2.3:5057949-5057968 None:intergenic 40.0%
GAAAAAACTCGCTCTGTTTC+TGG + chr2.3:5055840-5055859 None:intergenic 40.0%
GATTAGAATGAAACAGGAGG+AGG - chr2.3:5057363-5057382 MS.gene78368:intron 40.0%
GCAGGTTATGTTAGTATGAG+AGG - chr2.3:5056952-5056971 MS.gene78368:CDS 40.0%
GGGCCATTATATGTACATTG+AGG - chr2.3:5057805-5057824 MS.gene78368:CDS 40.0%
TATGAGGAAGCTACAAGATC+TGG - chr2.3:5057921-5057940 MS.gene78368:CDS 40.0%
! AAGGTACCATGCTTCATGAT+TGG - chr2.3:5057958-5057977 MS.gene78368:CDS 40.0%
! GAAGCTTTTGGTGAAAGTCA+TGG - chr2.3:5057129-5057148 MS.gene78368:intron 40.0%
! TTCATCTGAAATGGCAGCTA+TGG - chr2.3:5057431-5057450 MS.gene78368:intron 40.0%
!! AACCAAGTGGTTTGCTCAAA+TGG + chr2.3:5057671-5057690 None:intergenic 40.0%
!! ACATTCTGATTTGGCAAAGG+TGG + chr2.3:5056122-5056141 None:intergenic 40.0%
!! ATACCTGAGCTTGCTTTTGA+AGG + chr2.3:5058119-5058138 None:intergenic 40.0%
!! ATAGCTGAAGTTGGTGACAA+AGG - chr2.3:5057081-5057100 MS.gene78368:intron 40.0%
ACAAGATCTGGTCCCAAACA+TGG - chr2.3:5057933-5057952 MS.gene78368:CDS 45.0%
AGACATAGTAGTTCACCAGC+TGG - chr2.3:5056230-5056249 MS.gene78368:intron 45.0%
AGACCTTCAAAAGCAAGCTC+AGG - chr2.3:5058113-5058132 MS.gene78368:CDS 45.0%
AGATATGTTGGACTTGGCTG+TGG - chr2.3:5058080-5058099 MS.gene78368:CDS 45.0%
ATGAAAATTCGTGCCAAGCG+AGG - chr2.3:5057486-5057505 MS.gene78368:intron 45.0%
CACAGCAGATATGTTGGACT+TGG - chr2.3:5058074-5058093 MS.gene78368:CDS 45.0%
CAGCCAGTGAAGAATCATTG+TGG - chr2.3:5056188-5056207 MS.gene78368:intron 45.0%
TCTGGTCCCAAACATGGATA+AGG - chr2.3:5057939-5057958 MS.gene78368:CDS 45.0%
! CGCTTGGCACGAATTTTCAT+CGG + chr2.3:5057486-5057505 None:intergenic 45.0%
! GTTGTTGTTGCTGCTTGTGT+GGG + chr2.3:5058275-5058294 None:intergenic 45.0%
! TGTTGTTGTTGCTGCTTGTG+TGG + chr2.3:5058276-5058295 None:intergenic 45.0%
ACCCTTTCGGCAATGCTTCT+TGG + chr2.3:5057525-5057544 None:intergenic 50.0%
CCCTTTCGGCAATGCTTCTT+GGG + chr2.3:5057524-5057543 None:intergenic 50.0%
TTCGGCAATGCTTCTTGGGT+GGG + chr2.3:5057520-5057539 None:intergenic 50.0%
TTTCGGCAATGCTTCTTGGG+TGG + chr2.3:5057521-5057540 None:intergenic 50.0%
!! ACCCAAGAAGCATTGCCGAA+AGG - chr2.3:5057520-5057539 MS.gene78368:intron 50.0%
!! CCCAAGAAGCATTGCCGAAA+GGG - chr2.3:5057521-5057540 MS.gene78368:intron 50.0%
!! GAAGCATTGCCGAAAGGGTA+TGG - chr2.3:5057526-5057545 MS.gene78368:intron 50.0%
!! GACTTGTAAGGTACTGTCCG+TGG - chr2.3:5056581-5056600 MS.gene78368:CDS 50.0%
!! CGCTCTGTTTCTGGACTCGA+TGG + chr2.3:5055831-5055850 None:intergenic 55.0%
GGCAATGCTTCTTGGGTGGG+TGG + chr2.3:5057517-5057536 None:intergenic 60.0%
GTGGGTGGCACAACCTCGCT+TGG + chr2.3:5057502-5057521 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 5055696 5058299 5055696 ID=MS.gene78368
chr2.3 mRNA 5055696 5058299 5055696 ID=MS.gene78368.t1;Parent=MS.gene78368
chr2.3 exon 5057714 5058299 5057714 ID=MS.gene78368.t1.exon1;Parent=MS.gene78368.t1
chr2.3 CDS 5057714 5058299 5057714 ID=cds.MS.gene78368.t1;Parent=MS.gene78368.t1
chr2.3 exon 5056717 5057039 5056717 ID=MS.gene78368.t1.exon2;Parent=MS.gene78368.t1
chr2.3 CDS 5056717 5057039 5056717 ID=cds.MS.gene78368.t1;Parent=MS.gene78368.t1
chr2.3 exon 5056453 5056626 5056453 ID=MS.gene78368.t1.exon3;Parent=MS.gene78368.t1
chr2.3 CDS 5056453 5056626 5056453 ID=cds.MS.gene78368.t1;Parent=MS.gene78368.t1
chr2.3 exon 5056035 5056100 5056035 ID=MS.gene78368.t1.exon4;Parent=MS.gene78368.t1
chr2.3 CDS 5056035 5056100 5056035 ID=cds.MS.gene78368.t1;Parent=MS.gene78368.t1
chr2.3 exon 5055861 5055929 5055861 ID=MS.gene78368.t1.exon5;Parent=MS.gene78368.t1
chr2.3 CDS 5055861 5055929 5055861 ID=cds.MS.gene78368.t1;Parent=MS.gene78368.t1
chr2.3 exon 5055696 5055755 5055696 ID=MS.gene78368.t1.exon6;Parent=MS.gene78368.t1
chr2.3 CDS 5055696 5055755 5055696 ID=cds.MS.gene78368.t1;Parent=MS.gene78368.t1
Gene Sequence

>MS.gene78368

ATGGAGTCAGATCTTCATCAACAACAACACCCACAAGTAAATTCTTCAGGTTTAACAAGGTTTAAATCAGCACCAAGTTCATATTTCAACAACATCATTGACAGAGAATTCTACGAGCATGTTTTCAATAAACCATCGAGTCCAGAAACAGAGCGAGTTTTTTCAAGGTTTATTAACAGTTTTGGTTCAGAAGATGATTTACTAGCAGCTCAAAAAATCTCAGTAGATTCAACAGTGAAAGAAGAAGAAGAGGTTAATATGAATCATCAACAACAACAACAACAACAACAAGATCAGGGTTTGGCTTCAATCAACAATGAACATGTGGTTCATCAGCAGAGTAATATCAATATCAATATCAGTGATAATAATTATAACAACTCTGTTCCTTCTTCTCATGGTTTTTATCAAAGCTCTATGATGCCACCTTTGCCAAATCAGAATGTTTCTTCTGGTTTAGATGGAAGTTTTTCAATGGGAGTTAATAGGTTGCAGCCAGTGAAGAATCATTGTGGAAATAATTCAAATCTTATTAGACATAGTAGTTCACCAGCTGGATTATTTTCTCAAATTAACATTGAAAATGGTATGTTTCATATCTTCTAGTATGTGTTTTCTTTGTTACGCACTTTTGGTCCCTCTATTTTAATAGAAACGAGATTTAATTTTCGTTTCCAAATCTGAAATTTTAGTCACATTTTTCAAGTGTTTCAAGATTTTTATTTTGTACGACGTGTAATTACACTTGATTATGTGACATTACTGATTTTTGATCCGAAATGGTGGATTTTATTAGAATAAGTTTACATATTTGATCTATAAAATTGAAGAAAATGAATTGTAAAAGGTTTTCTGGTTAAAATTAGTACACGTTACTAGTATGTGGACTTGTAAGGTACTGTCCGTGGCATGTACAACTTCAATAAATTGATTTCAACCCAGTTTTCCTTGTCCTACTTTAAAGAGAAAAAAATATAATTTTCCTAGAAAAATGTTAAATTTGAAAATTTTCTTGTCAGGGGTTTTATGATGCTACTATATATATTAAAGATCTCTTTAGGGTAACATATACTTTTTAATATTTGCTATTATGTGGACATTATATTTTATACTTTGTACTTGTTGCAACAAGTGGTGAAAAAAAAAAACCCTAGTGGGAATATTAGTTATTACTTATTAGGCTTCATTTTTGTCCCCAAACTATTTTATTTCTTTAACATATAGATTGTTATAGTGATGTTATGCAAGTTTAATTGGCAGGTTATGTTAGTATGAGAGGAATGGGAACTTTAGGAGCTGTTAATAATTCTATGAAAGAACCAAAATTTTCTACTTCAAGGAATTTGAAGAATCAATCAAACTATTCATCAGGGTTAATGTCAACAATAGCTGAAGTTGGTGACAAAGGCAATAGAGAAAATAATGAAGAAAATGAAGCTTTTGGTGAAAGTCATGGTAACGAATATATGGATTATCAGGTTGATACTTGGGATGATTCTGAAATGATGTCTGAAAATGTTGGTGGTTTAAAAAGATTTAGAGATAATGATTCAAAACAACAATTTTCTGGTTTAAATGTTCAGGTAGAATACTATCTAGCATTGGTTAATTTGATCGTAAGTGTAGTTTCTTAATGGAATTTCATTTGACATGATTTTGTTATGGTTGATTAGAATGAAACAGGAGGAGGACATTCTAATTCTCCTTTGGCTCATCAATTGAGTATGCCAAATACTTCATCTGAAATGGCAGCTATGGAGAAGTTTCTGCATTTCTCAGATTCTGTTCCGATGAAAATTCGTGCCAAGCGAGGTTGTGCCACCCACCCAAGAAGCATTGCCGAAAGGGTATGGATTATTGATTTACAATATTTTTTTACCTTAGTTCTTATGAGGCAAGTAAAGTCTGATCAACGATTGTTTCAGTCAATATTTGAACTCAAGACAATTTGTCCTTAACCGAAGCTTATTAACCATTTGAGCAAACCACTTGGTTATAGATAATTCTAATTGACAACAATTTCCCTATATTACTTTTCTTTTGTTTAGGAAATCATAAACCAATTTACATATTTAGTTTTAGTATAGTACTATTAAGACATGATTTTTGGGGCCATTATATGTACATTGAGGTTCATTTTATTTGAGTTTACTAACTATATAAATTAATACTCTTAACACATCATTAAATTGGCTTCAGGTTAGAAGGACTAAAATTAGTGAGCGTATGAGGAAGCTACAAGATCTGGTCCCAAACATGGATAAGGTACCATGCTTCATGATTGGTTTTAACATTTGTAAAGTCTCAAACCATACATTAATTTATGTGAATGCTTTAAGATACAACGTTGTTTCTCTTCCTTTTTTGAAGCAAACAAACACAGCAGATATGTTGGACTTGGCTGTGGATTACATCAAAGACCTTCAAAAGCAAGCTCAGGTATTTCAAAATGCAATATCTCAACTATATATGTCTATATATATCTTTATCGTATTTATTATTATGAATTATTACCTAATTAATTTTCTTCCAACACTTGTATAGAAGCTTCAAGATTGTCAAGCAAAGTGTACGTGCCCACACAAGCAGCAACAACAACAATAA
Protein sequence

>MS.gene78368.t1

MESDLHQQQHPQVNSSGLTRFKSAPSSYFNNIIDREFYEHVFNKPSSPETERVFSRFINSFGSEDDLLAAQKISVDSTVKEEEEVNMNHQQQQQQQQDQGLASINNEHVVHQQSNININISDNNYNNSVPSSHGFYQSSMMPPLPNQNVSSGLDGSFSMGVNRLQPVKNHCGNNSNLIRHSSSPAGLFSQINIENGYVSMRGMGTLGAVNNSMKEPKFSTSRNLKNQSNYSSGLMSTIAEVGDKGNRENNEENEAFGESHGNEYMDYQVDTWDDSEMMSENVGGLKRFRDNDSKQQFSGLNVQNETGGGHSNSPLAHQLSMPNTSSEMAAMEKFLHFSDSVPMKIRAKRGCATHPRSIAERVRRTKISERMRKLQDLVPNMDKQTNTADMLDLAVDYIKDLQKQAQKLQDCQAKCTCPHKQQQQQ