Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78459.t1 | XP_003626285.1 | 70.4 | 162 | 13 | 2 | 1 | 132 | 274 | 430 | 4.70E-51 | 210.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78459.t1 | Q940P8 | 63.0 | 162 | 25 | 2 | 1 | 132 | 274 | 430 | 7.8e-47 | 188.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78459.t1 | G7KYN5 | 70.4 | 162 | 13 | 2 | 1 | 132 | 274 | 430 | 3.4e-51 | 210.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene78459 | 0.817083 | 3.96E-52 | -1.69E-46 |
| MS.gene050303 | MS.gene78459 | 0.815146 | 1.07E-51 | -1.69E-46 |
| MS.gene055808 | MS.gene78459 | 0.80181 | 7.55E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene78459 | 0.819873 | 9.24E-53 | -1.69E-46 |
| MS.gene05954 | MS.gene78459 | 0.810525 | 1.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78459.t1 | MTR_7g113470 | 70.370 | 162 | 13 | 2 | 1 | 132 | 274 | 430 | 8.23e-69 | 216 |
| MS.gene78459.t1 | MTR_7g067470 | 70.370 | 162 | 13 | 2 | 1 | 132 | 274 | 430 | 8.49e-69 | 216 |
| MS.gene78459.t1 | MTR_3g465910 | 72.727 | 55 | 12 | 1 | 81 | 132 | 453 | 507 | 4.11e-17 | 77.4 |
| MS.gene78459.t1 | MTR_5g087560 | 33.333 | 120 | 75 | 1 | 12 | 131 | 322 | 436 | 2.43e-13 | 66.6 |
| MS.gene78459.t1 | MTR_3g109925 | 34.483 | 116 | 65 | 3 | 34 | 143 | 292 | 402 | 4.27e-12 | 62.8 |
| MS.gene78459.t1 | MTR_3g109925 | 34.483 | 116 | 65 | 3 | 34 | 143 | 349 | 459 | 4.77e-12 | 62.8 |
| MS.gene78459.t1 | MTR_8g105970 | 33.621 | 116 | 67 | 3 | 34 | 143 | 206 | 317 | 9.62e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78459.t1 | AT5G20890 | 63.522 | 159 | 23 | 2 | 4 | 132 | 277 | 430 | 3.25e-58 | 189 |
| MS.gene78459.t1 | AT3G11830 | 33.051 | 118 | 74 | 1 | 14 | 131 | 324 | 436 | 5.34e-12 | 62.8 |
| MS.gene78459.t1 | AT3G18190 | 35.652 | 115 | 65 | 2 | 34 | 143 | 353 | 463 | 8.58e-12 | 62.0 |
Find 40 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTTGCCATTACCATTTC+AGG | 0.181073 | 7.1:-34141241 | None:intergenic |
| CTTATCGAAGATATTATTAT+TGG | 0.183032 | 7.1:+34140434 | MS.gene78459:CDS |
| GAGCGTCTGGCTCTGGTAAC+TGG | 0.301470 | 7.1:+34140353 | MS.gene78459:CDS |
| GGCAAAGGAAATTGATTCAT+TGG | 0.322602 | 7.1:+34141255 | MS.gene78459:CDS |
| GCATTATCAGCGATGGTTGT+TGG | 0.348817 | 7.1:-34141347 | None:intergenic |
| GAAAAGATGAACAAGATAAT+AGG | 0.380452 | 7.1:+34140311 | None:intergenic |
| CATTATCAGCGATGGTTGTT+GGG | 0.398556 | 7.1:-34141346 | None:intergenic |
| TGCCCAAGCTTAACAGACTC+TGG | 0.403665 | 7.1:-34140404 | None:intergenic |
| GGCACTTTCCCGAGCACTCT+TGG | 0.435170 | 7.1:+34141319 | MS.gene78459:CDS |
| TGGAGGCGGGTGGCCTGAAA+TGG | 0.444431 | 7.1:+34141228 | MS.gene78459:CDS |
| TTCTGGTGTTGCAATGTGGC+AGG | 0.456604 | 7.1:+34140478 | MS.gene78459:CDS |
| AATGACAGTAGGTTGTTACT+TGG | 0.472228 | 7.1:+34141208 | MS.gene78459:CDS |
| AACCCAGAGTCTGTTAAGCT+TGG | 0.477537 | 7.1:+34140401 | MS.gene78459:CDS |
| ACCCAGAGTCTGTTAAGCTT+GGG | 0.494078 | 7.1:+34140402 | MS.gene78459:CDS |
| TTGGGATAGCCAAGAGTGCT+CGG | 0.506902 | 7.1:-34141328 | None:intergenic |
| AAGTAACAACCTACTGTCAT+TGG | 0.509310 | 7.1:-34141206 | None:intergenic |
| AGTAGGTTGTTACTTGGAGG+CGG | 0.510127 | 7.1:+34141214 | MS.gene78459:CDS |
| GACAGTAGGTTGTTACTTGG+AGG | 0.514052 | 7.1:+34141211 | MS.gene78459:CDS |
| CGAAGATATTATTATTGGTG+AGG | 0.514475 | 7.1:+34140439 | MS.gene78459:CDS |
| AGGAAATTGATTCATTGGCT+AGG | 0.530474 | 7.1:+34141260 | MS.gene78459:CDS |
| GCCCAAGCTTAACAGACTCT+GGG | 0.530545 | 7.1:-34140403 | None:intergenic |
| CGGGTGGCCTGAAATGGTAA+TGG | 0.539649 | 7.1:+34141234 | MS.gene78459:CDS |
| AACAGACTCTGGGTTGTCAA+AGG | 0.543890 | 7.1:-34140393 | None:intergenic |
| GAGCCAGACGCTCAAAACAT+TGG | 0.559739 | 7.1:-34140343 | None:intergenic |
| AAGTCTCTTGCAAATGGAGG+AGG | 0.561240 | 7.1:+34141298 | MS.gene78459:CDS |
| GTAGGTTGTTACTTGGAGGC+GGG | 0.563910 | 7.1:+34141215 | MS.gene78459:CDS |
| GCCTGAAATGGTAATGGCAA+AGG | 0.576296 | 7.1:+34141240 | MS.gene78459:CDS |
| TGGGATAGCCAAGAGTGCTC+GGG | 0.583085 | 7.1:-34141327 | None:intergenic |
| GGTTGTTACTTGGAGGCGGG+TGG | 0.585391 | 7.1:+34141218 | MS.gene78459:CDS |
| ATGAACAAGATAATAGGTCA+TGG | 0.590525 | 7.1:+34140317 | MS.gene78459:CDS |
| TCATTGGCTAGGAAAACTCC+AGG | 0.591835 | 7.1:+34141271 | MS.gene78459:CDS |
| CAAACCAGCATTATCAGCGA+TGG | 0.610258 | 7.1:-34141354 | None:intergenic |
| CAGCCACCATGTTCTTGATG+AGG | 0.618440 | 7.1:+34141138 | MS.gene78459:intron |
| TCTCAGCCTCATCAAGAACA+TGG | 0.644225 | 7.1:-34141144 | None:intergenic |
| CTCAGACAGCCAATGACAGT+AGG | 0.655775 | 7.1:+34141197 | MS.gene78459:CDS |
| ATGTTCTTGATGAGGCTGAG+AGG | 0.658084 | 7.1:+34141146 | MS.gene78459:CDS |
| CGTCTGGCTCTGGTAACTGG+TGG | 0.662358 | 7.1:+34140356 | MS.gene78459:CDS |
| CAGCCTCATCAAGAACATGG+TGG | 0.689750 | 7.1:-34141141 | None:intergenic |
| TGTCTGAGATAGCACACACA+AGG | 0.706024 | 7.1:-34141182 | None:intergenic |
| GCATGCACAATAGTTCTGAG+AGG | 0.722642 | 7.1:+34140500 | MS.gene78459:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGCAGTAATTATTTTTTAAA+AGG | + | chr7.1:34140830-34140849 | MS.gene78459:intron | 15.0% |
| !! | CTTATCGAAGATATTATTAT+TGG | + | chr7.1:34140434-34140453 | MS.gene78459:CDS | 20.0% |
| !! | TATGTTTCATTATCTATTTG+TGG | + | chr7.1:34140669-34140688 | MS.gene78459:intron | 20.0% |
| !! | TCATAGTATTAGTAATTGAT+AGG | + | chr7.1:34140696-34140715 | MS.gene78459:intron | 20.0% |
| !!! | GATATATTGATTAAGTTTTC+TGG | + | chr7.1:34140461-34140480 | MS.gene78459:CDS | 20.0% |
| ! | GATGTTCATTTACATATTCA+AGG | + | chr7.1:34140570-34140589 | MS.gene78459:intron | 25.0% |
| ! | TATGTAAATGAACATCAGAA+AGG | - | chr7.1:34140566-34140585 | None:intergenic | 25.0% |
| !!! | TCATAGCATTTTTTCTTTTG+AGG | + | chr7.1:34140618-34140637 | MS.gene78459:intron | 25.0% |
| ACTGACTAGACCATAAAAAA+TGG | - | chr7.1:34140990-34141009 | None:intergenic | 30.0% | |
| ATGAACAAGATAATAGGTCA+TGG | + | chr7.1:34140317-34140336 | MS.gene78459:CDS | 30.0% | |
| ATTGAGTCCAATGATTTAAC+TGG | + | chr7.1:34140874-34140893 | MS.gene78459:intron | 30.0% | |
| CAGCATAGAAACTGAATATA+AGG | - | chr7.1:34140742-34140761 | None:intergenic | 30.0% | |
| CGAAGATATTATTATTGGTG+AGG | + | chr7.1:34140439-34140458 | MS.gene78459:CDS | 30.0% | |
| CTAGACCATAAAAAATGGTA+AGG | - | chr7.1:34140985-34141004 | None:intergenic | 30.0% | |
| CTTATATTCAGTTTCTATGC+TGG | + | chr7.1:34140740-34140759 | MS.gene78459:intron | 30.0% | |
| !! | TTTCTATGCTGGTTTATTGA+AGG | + | chr7.1:34140751-34140770 | MS.gene78459:intron | 30.0% |
| !!! | CTTTTGAGGTTTTCTTATTC+TGG | + | chr7.1:34140632-34140651 | MS.gene78459:intron | 30.0% |
| AAAAAGTCTCTTGCAAATGG+AGG | + | chr7.1:34141295-34141314 | MS.gene78459:CDS | 35.0% | |
| AAGTAACAACCTACTGTCAT+TGG | - | chr7.1:34141209-34141228 | None:intergenic | 35.0% | |
| AATGACAGTAGGTTGTTACT+TGG | + | chr7.1:34141208-34141227 | MS.gene78459:CDS | 35.0% | |
| CATAAAAAATGGTAAGGAGC+TGG | - | chr7.1:34140979-34140998 | None:intergenic | 35.0% | |
| GGAAAAAAGTCTCTTGCAAA+TGG | + | chr7.1:34141292-34141311 | MS.gene78459:CDS | 35.0% | |
| ! | AGGAAATTGATTCATTGGCT+AGG | + | chr7.1:34141260-34141279 | MS.gene78459:CDS | 35.0% |
| ! | GGCAAAGGAAATTGATTCAT+TGG | + | chr7.1:34141255-34141274 | MS.gene78459:CDS | 35.0% |
| !!! | CAGCTCCTTACCATTTTTTA+TGG | + | chr7.1:34140977-34140996 | MS.gene78459:intron | 35.0% |
| !!! | TTTGCAAGAGACTTTTTTCC+TGG | - | chr7.1:34141292-34141311 | None:intergenic | 35.0% |
| CAGCAGTCCAGTTAAATCAT+TGG | - | chr7.1:34140884-34140903 | None:intergenic | 40.0% | |
| CTGTGACAAAAACAAACGTC+AGG | - | chr7.1:34141121-34141140 | None:intergenic | 40.0% | |
| GCATTTGAGCTGAACATGTA+TGG | + | chr7.1:34141010-34141029 | MS.gene78459:intron | 40.0% | |
| GTAAGGAGCTGGTGAATATA+AGG | - | chr7.1:34140968-34140987 | None:intergenic | 40.0% | |
| TAACTGGACTGCTGAAATGT+TGG | + | chr7.1:34140890-34140909 | MS.gene78459:intron | 40.0% | |
| TCCTTTGCCATTACCATTTC+AGG | - | chr7.1:34141244-34141263 | None:intergenic | 40.0% | |
| !!! | AGTTTTCTGGTGTTGCAATG+TGG | + | chr7.1:34140474-34140493 | MS.gene78459:CDS | 40.0% |
| AACAGACTCTGGGTTGTCAA+AGG | - | chr7.1:34140396-34140415 | None:intergenic | 45.0% | |
| AACCCAGAGTCTGTTAAGCT+TGG | + | chr7.1:34140401-34140420 | MS.gene78459:CDS | 45.0% | |
| AAGTCTCTTGCAAATGGAGG+AGG | + | chr7.1:34141298-34141317 | MS.gene78459:CDS | 45.0% | |
| ACCCAGAGTCTGTTAAGCTT+GGG | + | chr7.1:34140402-34140421 | MS.gene78459:CDS | 45.0% | |
| ATGTTCTTGATGAGGCTGAG+AGG | + | chr7.1:34141146-34141165 | MS.gene78459:CDS | 45.0% | |
| TCTCAGCCTCATCAAGAACA+TGG | - | chr7.1:34141147-34141166 | None:intergenic | 45.0% | |
| ! | AGTAGGTTGTTACTTGGAGG+CGG | + | chr7.1:34141214-34141233 | MS.gene78459:CDS | 45.0% |
| ! | GACAGTAGGTTGTTACTTGG+AGG | + | chr7.1:34141211-34141230 | MS.gene78459:CDS | 45.0% |
| ! | TCATTGGCTAGGAAAACTCC+AGG | + | chr7.1:34141271-34141290 | MS.gene78459:CDS | 45.0% |
| ! | TGTCTGAGATAGCACACACA+AGG | - | chr7.1:34141185-34141204 | None:intergenic | 45.0% |
| !! | CATCCAATGTTTTGAGCGTC+TGG | + | chr7.1:34140340-34140359 | MS.gene78459:CDS | 45.0% |
| !! | GCATGCACAATAGTTCTGAG+AGG | + | chr7.1:34140500-34140519 | MS.gene78459:CDS | 45.0% |
| !! | GCCTGAAATGGTAATGGCAA+AGG | + | chr7.1:34141240-34141259 | MS.gene78459:CDS | 45.0% |
| CAGCCACCATGTTCTTGATG+AGG | + | chr7.1:34141138-34141157 | MS.gene78459:intron | 50.0% | |
| CAGCCTCATCAAGAACATGG+TGG | - | chr7.1:34141144-34141163 | None:intergenic | 50.0% | |
| CTCAGACAGCCAATGACAGT+AGG | + | chr7.1:34141197-34141216 | MS.gene78459:CDS | 50.0% | |
| GAGCCAGACGCTCAAAACAT+TGG | - | chr7.1:34140346-34140365 | None:intergenic | 50.0% | |
| GCCCAAGCTTAACAGACTCT+GGG | - | chr7.1:34140406-34140425 | None:intergenic | 50.0% | |
| TGCCCAAGCTTAACAGACTC+TGG | - | chr7.1:34140407-34140426 | None:intergenic | 50.0% | |
| ! | GTAGGTTGTTACTTGGAGGC+GGG | + | chr7.1:34141215-34141234 | MS.gene78459:CDS | 50.0% |
| !! | ATGTTTTGAGCGTCTGGCTC+TGG | + | chr7.1:34140346-34140365 | MS.gene78459:CDS | 50.0% |
| !! | TTCTGGTGTTGCAATGTGGC+AGG | + | chr7.1:34140478-34140497 | MS.gene78459:CDS | 50.0% |
| !! | TTGGGATAGCCAAGAGTGCT+CGG | - | chr7.1:34141331-34141350 | None:intergenic | 50.0% |
| CGGGTGGCCTGAAATGGTAA+TGG | + | chr7.1:34141234-34141253 | MS.gene78459:CDS | 55.0% | |
| !! | AGTTCTGAGAGGTGCGTGCT+AGG | + | chr7.1:34140511-34140530 | MS.gene78459:intron | 55.0% |
| !! | TGGGATAGCCAAGAGTGCTC+GGG | - | chr7.1:34141330-34141349 | None:intergenic | 55.0% |
| GGCACTTTCCCGAGCACTCT+TGG | + | chr7.1:34141319-34141338 | MS.gene78459:CDS | 60.0% | |
| ! | GGTTGTTACTTGGAGGCGGG+TGG | + | chr7.1:34141218-34141237 | MS.gene78459:CDS | 60.0% |
| !! | CGTCTGGCTCTGGTAACTGG+TGG | + | chr7.1:34140356-34140375 | MS.gene78459:CDS | 60.0% |
| !! | GAGCGTCTGGCTCTGGTAAC+TGG | + | chr7.1:34140353-34140372 | MS.gene78459:CDS | 60.0% |
| TGGAGGCGGGTGGCCTGAAA+TGG | + | chr7.1:34141228-34141247 | MS.gene78459:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 34140317 | 34141363 | 34140317 | ID=MS.gene78459 |
| chr7.1 | mRNA | 34140317 | 34141363 | 34140317 | ID=MS.gene78459.t1;Parent=MS.gene78459 |
| chr7.1 | exon | 34140317 | 34140521 | 34140317 | ID=MS.gene78459.t1.exon1;Parent=MS.gene78459.t1 |
| chr7.1 | CDS | 34140317 | 34140521 | 34140317 | ID=cds.MS.gene78459.t1;Parent=MS.gene78459.t1 |
| chr7.1 | exon | 34141005 | 34141026 | 34141005 | ID=MS.gene78459.t1.exon2;Parent=MS.gene78459.t1 |
| chr7.1 | CDS | 34141005 | 34141026 | 34141005 | ID=cds.MS.gene78459.t1;Parent=MS.gene78459.t1 |
| chr7.1 | exon | 34141141 | 34141363 | 34141141 | ID=MS.gene78459.t1.exon3;Parent=MS.gene78459.t1 |
| chr7.1 | CDS | 34141141 | 34141363 | 34141141 | ID=cds.MS.gene78459.t1;Parent=MS.gene78459.t1 |
| Gene Sequence |
| Protein sequence |
