Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78680.t1 | KEH19202.1 | 94.4 | 195 | 11 | 0 | 1 | 195 | 315 | 509 | 3.60E-99 | 370.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78680.t1 | Q9LNU1 | 46.2 | 195 | 105 | 0 | 1 | 195 | 314 | 508 | 3.1e-43 | 176.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78680.t1 | A0A072TP65 | 94.4 | 195 | 11 | 0 | 1 | 195 | 315 | 509 | 2.6e-99 | 370.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050338 | MS.gene78680 | 0.805551 | 1.26E-49 | -1.69E-46 |
| MS.gene051863 | MS.gene78680 | 0.807372 | 5.22E-50 | -1.69E-46 |
| MS.gene056694 | MS.gene78680 | 0.800763 | 1.24E-48 | -1.69E-46 |
| MS.gene056697 | MS.gene78680 | 0.804275 | 2.34E-49 | -1.69E-46 |
| MS.gene056856 | MS.gene78680 | 0.813904 | 2.02E-51 | -1.69E-46 |
| MS.gene060405 | MS.gene78680 | 0.821605 | 3.69E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTAGAAACTTCTGTTGT+TGG | 0.344793 | 8.4:+57003561 | None:intergenic |
| CAATTTCCCTTCACAGAAGT+TGG | 0.371509 | 8.4:-57003980 | MS.gene78680:CDS |
| ACAGAAGTTGGCAATCTTGA+AGG | 0.402252 | 8.4:-57003968 | MS.gene78680:CDS |
| AAAACATCGTTGAGCGAGTA+AGG | 0.418445 | 8.4:+57004515 | None:intergenic |
| TTCCAAGGACAACGGTTGAC+TGG | 0.418496 | 8.4:+57004784 | None:intergenic |
| TTCCAGTCAACCGTTGTCCT+TGG | 0.423270 | 8.4:-57004786 | MS.gene78680:CDS |
| TTATGTACTTAAGGATTTGA+AGG | 0.434534 | 8.4:+57003945 | None:intergenic |
| CAACCGTTGTCCTTGGAAAC+GGG | 0.442265 | 8.4:-57004779 | MS.gene78680:CDS |
| GGGAAATTGACCTGCATCAA+AGG | 0.447674 | 8.4:+57003994 | None:intergenic |
| CAACAGAAGTTTCTAGATAC+AGG | 0.450148 | 8.4:-57003557 | MS.gene78680:CDS |
| TCAACCGTTGTCCTTGGAAA+CGG | 0.458295 | 8.4:-57004780 | MS.gene78680:CDS |
| GTGTGTTCAGCTGGGAATGA+TGG | 0.474923 | 8.4:-57004882 | None:intergenic |
| AAATGCAGGAACTGTTATCC+TGG | 0.487437 | 8.4:-57004163 | MS.gene78680:intron |
| TTTACAAGATGCGGGAGCCG+TGG | 0.518953 | 8.4:-57004053 | MS.gene78680:CDS |
| AAGATTGCCAACTTCTGTGA+AGG | 0.525151 | 8.4:+57003973 | None:intergenic |
| TGTTTGTGTTGTCAGGGTGT+TGG | 0.526384 | 8.4:-57004553 | MS.gene78680:intron |
| TTACAAGATGCGGGAGCCGT+GGG | 0.529726 | 8.4:-57004052 | MS.gene78680:CDS |
| AAGCAACAAAATCGGAAGCT+AGG | 0.532845 | 8.4:-57004459 | MS.gene78680:intron |
| TAAAAGCTTTCCCGTTTCCA+AGG | 0.540160 | 8.4:+57004769 | None:intergenic |
| GTTGCATCTTTCTCATCAAG+AGG | 0.542476 | 8.4:-57003522 | MS.gene78680:CDS |
| GGAAATTGACCTGCATCAAA+GGG | 0.544423 | 8.4:+57003995 | None:intergenic |
| AAAGATGCAACAATCGGTGC+AGG | 0.544876 | 8.4:+57003534 | None:intergenic |
| ATAGATCCTCCCTTTGATGC+AGG | 0.549218 | 8.4:-57004004 | MS.gene78680:CDS |
| GTTGGAAGAATTGTCGCAGT+TGG | 0.555948 | 8.4:+57003579 | None:intergenic |
| GATGAGAAAGATGCAACAAT+CGG | 0.562687 | 8.4:+57003528 | None:intergenic |
| AGATTGCCAACTTCTGTGAA+GGG | 0.567860 | 8.4:+57003974 | None:intergenic |
| TTGGCAACACTGTAATGATC+GGG | 0.581092 | 8.4:+57004858 | None:intergenic |
| TTGCAGCTTCTAATATCGAT+AGG | 0.600561 | 8.4:-57004812 | MS.gene78680:CDS |
| CTCAATGAGTATCATCCCCA+CGG | 0.606435 | 8.4:+57004036 | None:intergenic |
| CCTTACTGAAAACATTCTCA+AGG | 0.609833 | 8.4:-57003490 | MS.gene78680:CDS |
| GCTGCAACTGTAAATATCCA+TGG | 0.617364 | 8.4:+57004828 | None:intergenic |
| AAATATCCATGGCGCTGTGT+TGG | 0.621235 | 8.4:+57004839 | None:intergenic |
| GTGTTGCCAACACAGCGCCA+TGG | 0.627231 | 8.4:-57004845 | MS.gene78680:CDS |
| GTTGGCAACACTGTAATGAT+CGG | 0.639619 | 8.4:+57004857 | None:intergenic |
| AATTGACCTGCATCAAAGGG+AGG | 0.672580 | 8.4:+57003998 | None:intergenic |
| TACAAGATGCGGGAGCCGTG+GGG | 0.673950 | 8.4:-57004051 | MS.gene78680:CDS |
| TTTCCCGTTTCCAAGGACAA+CGG | 0.678603 | 8.4:+57004776 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGATAACTAAGTTATTTT+AGG | + | chr8.4:57003972-57003991 | None:intergenic | 15.0% |
| !!! | ATGTTTTCTTCAATTAATAT+CGG | + | chr8.4:57003708-57003727 | None:intergenic | 15.0% |
| !! | AAATGTTAAATGCATAAACA+AGG | - | chr8.4:57004481-57004500 | MS.gene78680:CDS | 20.0% |
| !! | ATTGAAGAAAACATAATTGT+CGG | - | chr8.4:57003714-57003733 | MS.gene78680:intron | 20.0% |
| !!! | AATATTGTTTTTGTTGTTGT+TGG | - | chr8.4:57004024-57004043 | MS.gene78680:CDS | 20.0% |
| !!! | AGTTATTTTAGGCTTAAAAA+AGG | + | chr8.4:57003961-57003980 | None:intergenic | 20.0% |
| !!! | ATTGCAATTACAATATTTTG+TGG | - | chr8.4:57004522-57004541 | MS.gene78680:CDS | 20.0% |
| ! | TTATGTACTTAAGGATTTGA+AGG | + | chr8.4:57004409-57004428 | None:intergenic | 25.0% |
| !! | TGGTAGATTTTATGTACTTA+AGG | + | chr8.4:57004418-57004437 | None:intergenic | 25.0% |
| !!! | ATAAGTGTTTTAAACTGTGA+CGG | + | chr8.4:57004588-57004607 | None:intergenic | 25.0% |
| !!! | CATTCTATCTATAAGTTGAT+TGG | - | chr8.4:57004688-57004707 | MS.gene78680:intron | 25.0% |
| !!! | GTTGATGACATAAAACTAAT+TGG | + | chr8.4:57004078-57004097 | None:intergenic | 25.0% |
| !!! | TGTTTTTATGCAAATGATGA+TGG | + | chr8.4:57004449-57004468 | None:intergenic | 25.0% |
| AGGTGAACATCTTTGTATAA+AGG | - | chr8.4:57004626-57004645 | MS.gene78680:intron | 30.0% | |
| ATTGAATGCTAAAGCCTAAA+AGG | - | chr8.4:57004606-57004625 | MS.gene78680:intron | 30.0% | |
| CTAGATAACAAAAAAGTTGC+AGG | - | chr8.4:57004215-57004234 | MS.gene78680:intron | 30.0% | |
| TATCTAGAAACTTCTGTTGT+TGG | + | chr8.4:57004793-57004812 | None:intergenic | 30.0% | |
| TGCCAAAAAAGCAACAAAAT+CGG | - | chr8.4:57003884-57003903 | MS.gene78680:intron | 30.0% | |
| TTCGTACTTCATACATAGTT+AGG | + | chr8.4:57003650-57003669 | None:intergenic | 30.0% | |
| ! | AAGCTAATTTTACAAGATGC+GGG | - | chr8.4:57004290-57004309 | MS.gene78680:intron | 30.0% |
| ! | GAAGCTAATTTTACAAGATG+CGG | - | chr8.4:57004289-57004308 | MS.gene78680:intron | 30.0% |
| ! | TACAAAGATGTTCACCTTTT+AGG | + | chr8.4:57004623-57004642 | None:intergenic | 30.0% |
| !! | AAATGATGATGGTACTTACT+TGG | + | chr8.4:57004438-57004457 | None:intergenic | 30.0% |
| !! | TGAAAATGTCTCTAAAATGC+AGG | - | chr8.4:57004174-57004193 | MS.gene78680:intron | 30.0% |
| !! | TTTTTGTTATCTAGTGATCC+AGG | + | chr8.4:57004209-57004228 | None:intergenic | 30.0% |
| !!! | ACGATGTTTTCTCTTGTTTT+TGG | - | chr8.4:57003849-57003868 | MS.gene78680:intron | 30.0% |
| !!! | TTCCGATTTTGTTGCTTTTT+TGG | + | chr8.4:57003889-57003908 | None:intergenic | 30.0% |
| AACCTATCATGTTCTGTTAC+AGG | - | chr8.4:57004746-57004765 | MS.gene78680:intron | 35.0% | |
| CCTTACTGAAAACATTCTCA+AGG | - | chr8.4:57004861-57004880 | MS.gene78680:CDS | 35.0% | |
| GATGAGAAAGATGCAACAAT+CGG | + | chr8.4:57004826-57004845 | None:intergenic | 35.0% | |
| TTCCTGTAACAGAACATGAT+AGG | + | chr8.4:57004751-57004770 | None:intergenic | 35.0% | |
| TTGCAGCTTCTAATATCGAT+AGG | - | chr8.4:57003539-57003558 | MS.gene78680:CDS | 35.0% | |
| ! | ATGTTTTCAGTAAGGCTTGA+AGG | + | chr8.4:57004856-57004875 | None:intergenic | 35.0% |
| ! | CAACAGAAGTTTCTAGATAC+AGG | - | chr8.4:57004794-57004813 | MS.gene78680:CDS | 35.0% |
| ! | CCTTGAGAATGTTTTCAGTA+AGG | + | chr8.4:57004864-57004883 | None:intergenic | 35.0% |
| ! | TAGGTCTATCGTCATTCTTT+TGG | + | chr8.4:57003631-57003650 | None:intergenic | 35.0% |
| ! | TGATAGGTTGAGCATATCTA+TGG | + | chr8.4:57004735-57004754 | None:intergenic | 35.0% |
| !!! | GTTTTCTCTTGTTTTTGGAG+AGG | - | chr8.4:57003854-57003873 | MS.gene78680:intron | 35.0% |
| AAAACATCGTTGAGCGAGTA+AGG | + | chr8.4:57003839-57003858 | None:intergenic | 40.0% | |
| AAATGCAGGAACTGTTATCC+TGG | - | chr8.4:57004188-57004207 | MS.gene78680:intron | 40.0% | |
| AAGATTGCCAACTTCTGTGA+AGG | + | chr8.4:57004381-57004400 | None:intergenic | 40.0% | |
| AAGCAACAAAATCGGAAGCT+AGG | - | chr8.4:57003892-57003911 | MS.gene78680:intron | 40.0% | |
| ACAGAAGTTGGCAATCTTGA+AGG | - | chr8.4:57004383-57004402 | MS.gene78680:intron | 40.0% | |
| ACCCTGACAACACAAACAAA+CGG | + | chr8.4:57003796-57003815 | None:intergenic | 40.0% | |
| AGATTGCCAACTTCTGTGAA+GGG | + | chr8.4:57004380-57004399 | None:intergenic | 40.0% | |
| CAATTTCCCTTCACAGAAGT+TGG | - | chr8.4:57004371-57004390 | MS.gene78680:intron | 40.0% | |
| CTTCTGTCAACTAGTATCAG+AGG | + | chr8.4:57004140-57004159 | None:intergenic | 40.0% | |
| GCTGCAACTGTAAATATCCA+TGG | + | chr8.4:57003526-57003545 | None:intergenic | 40.0% | |
| GGAAATTGACCTGCATCAAA+GGG | + | chr8.4:57004359-57004378 | None:intergenic | 40.0% | |
| GTTGCATCTTTCTCATCAAG+AGG | - | chr8.4:57004829-57004848 | MS.gene78680:CDS | 40.0% | |
| GTTGGCAACACTGTAATGAT+CGG | + | chr8.4:57003497-57003516 | None:intergenic | 40.0% | |
| TAAAAGCTTTCCCGTTTCCA+AGG | + | chr8.4:57003585-57003604 | None:intergenic | 40.0% | |
| TCCGTTTGTTTGTGTTGTCA+GGG | - | chr8.4:57003792-57003811 | MS.gene78680:intron | 40.0% | |
| TTCCGTTTGTTTGTGTTGTC+AGG | - | chr8.4:57003791-57003810 | MS.gene78680:intron | 40.0% | |
| TTGGCAACACTGTAATGATC+GGG | + | chr8.4:57003496-57003515 | None:intergenic | 40.0% | |
| !! | CTATCGTCATTCTTTTGGCT+TGG | + | chr8.4:57003626-57003645 | None:intergenic | 40.0% |
| AAAGATGCAACAATCGGTGC+AGG | + | chr8.4:57004820-57004839 | None:intergenic | 45.0% | |
| AAATATCCATGGCGCTGTGT+TGG | + | chr8.4:57003515-57003534 | None:intergenic | 45.0% | |
| AATTGACCTGCATCAAAGGG+AGG | + | chr8.4:57004356-57004375 | None:intergenic | 45.0% | |
| ATAGATCCTCCCTTTGATGC+AGG | - | chr8.4:57004347-57004366 | MS.gene78680:intron | 45.0% | |
| CTCAATGAGTATCATCCCCA+CGG | + | chr8.4:57004318-57004337 | None:intergenic | 45.0% | |
| GGGAAATTGACCTGCATCAA+AGG | + | chr8.4:57004360-57004379 | None:intergenic | 45.0% | |
| GTTGGAAGAATTGTCGCAGT+TGG | + | chr8.4:57004775-57004794 | None:intergenic | 45.0% | |
| TCAACCGTTGTCCTTGGAAA+CGG | - | chr8.4:57003571-57003590 | MS.gene78680:CDS | 45.0% | |
| TGTCGGATACTTACACGTGT+TGG | - | chr8.4:57003731-57003750 | MS.gene78680:intron | 45.0% | |
| TGTTTGTGTTGTCAGGGTGT+TGG | - | chr8.4:57003798-57003817 | MS.gene78680:intron | 45.0% | |
| TTTCCCGTTTCCAAGGACAA+CGG | + | chr8.4:57003578-57003597 | None:intergenic | 45.0% | |
| !! | AATAATATAAGTAAATAAAA+TGG | - | chr8.4:57004652-57004671 | MS.gene78680:intron | 5.0% |
| CAACCGTTGTCCTTGGAAAC+GGG | - | chr8.4:57003572-57003591 | MS.gene78680:CDS | 50.0% | |
| TTCCAAGGACAACGGTTGAC+TGG | + | chr8.4:57003570-57003589 | None:intergenic | 50.0% | |
| TTCCAGTCAACCGTTGTCCT+TGG | - | chr8.4:57003565-57003584 | MS.gene78680:CDS | 50.0% | |
| TTACAAGATGCGGGAGCCGT+GGG | - | chr8.4:57004299-57004318 | MS.gene78680:intron | 55.0% | |
| TTTACAAGATGCGGGAGCCG+TGG | - | chr8.4:57004298-57004317 | MS.gene78680:intron | 55.0% | |
| GTGTTGCCAACACAGCGCCA+TGG | - | chr8.4:57003506-57003525 | MS.gene78680:CDS | 60.0% | |
| TACAAGATGCGGGAGCCGTG+GGG | - | chr8.4:57004300-57004319 | MS.gene78680:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 57003479 | 57004894 | 57003479 | ID=MS.gene78680 |
| chr8.4 | mRNA | 57003479 | 57004894 | 57003479 | ID=MS.gene78680.t1;Parent=MS.gene78680 |
| chr8.4 | exon | 57004767 | 57004894 | 57004767 | ID=MS.gene78680.t1.exon1;Parent=MS.gene78680.t1 |
| chr8.4 | CDS | 57004767 | 57004894 | 57004767 | ID=cds.MS.gene78680.t1;Parent=MS.gene78680.t1 |
| chr8.4 | exon | 57004460 | 57004560 | 57004460 | ID=MS.gene78680.t1.exon2;Parent=MS.gene78680.t1 |
| chr8.4 | CDS | 57004460 | 57004560 | 57004460 | ID=cds.MS.gene78680.t1;Parent=MS.gene78680.t1 |
| chr8.4 | exon | 57003935 | 57004177 | 57003935 | ID=MS.gene78680.t1.exon3;Parent=MS.gene78680.t1 |
| chr8.4 | CDS | 57003935 | 57004177 | 57003935 | ID=cds.MS.gene78680.t1;Parent=MS.gene78680.t1 |
| chr8.4 | exon | 57003479 | 57003605 | 57003479 | ID=MS.gene78680.t1.exon4;Parent=MS.gene78680.t1 |
| chr8.4 | CDS | 57003479 | 57003605 | 57003479 | ID=cds.MS.gene78680.t1;Parent=MS.gene78680.t1 |
| Gene Sequence |
| Protein sequence |
