Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78744.t1 | XP_013452692.1 | 86.4 | 369 | 45 | 3 | 1 | 365 | 1 | 368 | 5.40E-133 | 484.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78744.t1 | A0A072ULL0 | 86.4 | 369 | 45 | 3 | 1 | 365 | 1 | 368 | 3.9e-133 | 484.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene78744 | 0.817167 | 3.80E-52 | -1.69E-46 |
| MS.gene053931 | MS.gene78744 | 0.894371 | 2.63E-75 | -1.69E-46 |
| MS.gene054892 | MS.gene78744 | 0.810436 | 1.15E-50 | -1.69E-46 |
| MS.gene054893 | MS.gene78744 | 0.807749 | 4.34E-50 | -1.69E-46 |
| MS.gene057622 | MS.gene78744 | 0.829497 | 4.98E-55 | -1.69E-46 |
| MS.gene060244 | MS.gene78744 | 0.805802 | 1.12E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78744.t1 | MTR_6g472100 | 97.561 | 369 | 4 | 3 | 1 | 365 | 1 | 368 | 0.0 | 713 |
| MS.gene78744.t1 | MTR_6g046300 | 93.478 | 46 | 1 | 2 | 223 | 268 | 36 | 79 | 4.59e-18 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78744.t1 | AT3G25590 | 36.465 | 447 | 147 | 19 | 1 | 348 | 1 | 409 | 2.02e-54 | 185 |
Find 57 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGAGGTGGAGCTATATT+TGG | 0.183362 | 5.1:-41903821 | MS.gene78744:CDS |
| GGAGAATTGGGAAACTATTT+AGG | 0.222773 | 5.1:-41903958 | MS.gene78744:CDS |
| TAGAAGAAATCACTTCTTCT+TGG | 0.257265 | 5.1:+41903671 | None:intergenic |
| TTCTTCTATGAATATGAAAA+TGG | 0.271087 | 5.1:-41903656 | MS.gene78744:CDS |
| GGTGGTGGTGGTGGAGGTGA+TGG | 0.288439 | 5.1:-41903428 | MS.gene78744:CDS |
| GCAGATACACTCTCAGCTTT+TGG | 0.291697 | 5.1:-41903491 | MS.gene78744:CDS |
| ATTTCAGCATTTGAATTCTA+AGG | 0.291707 | 5.1:-41904068 | MS.gene78744:CDS |
| TTCTTCTTTCTCTTGCATCT+TGG | 0.305233 | 5.1:+41904045 | None:intergenic |
| TTAGAATTCAAATGCTGAAA+TGG | 0.334316 | 5.1:+41904070 | None:intergenic |
| TCAAATATCATTCAAGTTCA+AGG | 0.341466 | 5.1:-41903325 | MS.gene78744:CDS |
| ATTTCTGCAGCTAGAAGTTC+AGG | 0.351861 | 5.1:-41903752 | MS.gene78744:CDS |
| TAGAATTCAAATGCTGAAAT+GGG | 0.399760 | 5.1:+41904071 | None:intergenic |
| CATGATGCTGGTGGTGGTGG+TGG | 0.415319 | 5.1:-41903440 | MS.gene78744:CDS |
| TTACTGTCTTAAAGAACGTT+TGG | 0.432017 | 5.1:-41904245 | MS.gene78744:CDS |
| GGTGGTGGTGGAGGTGATGG+AGG | 0.453840 | 5.1:-41903425 | MS.gene78744:CDS |
| ATCATCATGGAGGTGTTTCA+AGG | 0.475029 | 5.1:-41903862 | MS.gene78744:CDS |
| TAGAAAGAGTACTGGTGTAA+TGG | 0.476645 | 5.1:-41903633 | MS.gene78744:CDS |
| GCAAGAGAAAGAAGAAGAAG+AGG | 0.479357 | 5.1:-41904038 | MS.gene78744:CDS |
| TTGATGGAAGCAGCTACAAT+AGG | 0.481008 | 5.1:-41903607 | MS.gene78744:CDS |
| GAAAGAAGAAGAAGAGGTTG+TGG | 0.494932 | 5.1:-41904032 | MS.gene78744:CDS |
| GGTGGTTTGATGTTAGAATC+TGG | 0.497991 | 5.1:-41903722 | MS.gene78744:CDS |
| TGAATGAAAGAGGAATTAAG+AGG | 0.498145 | 5.1:-41903385 | MS.gene78744:CDS |
| AACAGAGCATGAATTTCGAT+TGG | 0.499202 | 5.1:+41904153 | None:intergenic |
| ACACATGATGCTGGTGGTGG+TGG | 0.503836 | 5.1:-41903443 | MS.gene78744:CDS |
| ACTGGTGTAATGGAAATTGA+TGG | 0.506563 | 5.1:-41903623 | MS.gene78744:CDS |
| AAGCTTGTGTGTTCAGATTG+TGG | 0.522398 | 5.1:-41904220 | MS.gene78744:CDS |
| AAAGTATTAAGGGTTGGAGA+TGG | 0.524569 | 5.1:-41903352 | MS.gene78744:CDS |
| GAAAATGGTAGAAAGAGTAC+TGG | 0.527029 | 5.1:-41903641 | MS.gene78744:CDS |
| AATAAGAAGAACAAGGATTG+TGG | 0.528290 | 5.1:-41903932 | MS.gene78744:CDS |
| GAAGAAGGATGCTTCTTACA+TGG | 0.543996 | 5.1:+41904292 | None:intergenic |
| GTTTGATGTTAGAATCTGGT+AGG | 0.549457 | 5.1:-41903718 | MS.gene78744:CDS |
| GAATGAAAGAGGAATTAAGA+GGG | 0.561877 | 5.1:-41903384 | MS.gene78744:CDS |
| ATGACACATGATGCTGGTGG+TGG | 0.566242 | 5.1:-41903446 | MS.gene78744:CDS |
| GCTAGAAGTTCAGGTGTGAA+TGG | 0.567727 | 5.1:-41903743 | MS.gene78744:CDS |
| TTAGAATCTGGTAGGAGAAG+TGG | 0.568091 | 5.1:-41903710 | MS.gene78744:CDS |
| AGATCTCTCTGCAGTTTCAG+AGG | 0.571126 | 5.1:-41903836 | MS.gene78744:CDS |
| AAGGATTGTGGTAGAAGTGT+TGG | 0.572137 | 5.1:-41903920 | MS.gene78744:CDS |
| TCGAAATTCATGCTCTGTTG+AGG | 0.575005 | 5.1:-41904149 | MS.gene78744:CDS |
| GCTATATTTGGATCTGAAGA+TGG | 0.581428 | 5.1:-41903809 | MS.gene78744:CDS |
| AATTTCATGACACATGATGC+TGG | 0.583341 | 5.1:-41903452 | MS.gene78744:CDS |
| ATGTGGATGGTTGATCATCA+TGG | 0.586021 | 5.1:-41903875 | MS.gene78744:CDS |
| TAGGAAGAATAAGAAGAACA+AGG | 0.590362 | 5.1:-41903939 | MS.gene78744:CDS |
| AGGTTCACTATGAATGAAAG+AGG | 0.598015 | 5.1:-41903395 | MS.gene78744:CDS |
| AATTCATGCTCTGTTGAGGT+AGG | 0.600288 | 5.1:-41904145 | MS.gene78744:CDS |
| TTGATGAGAAGAATGAGATG+TGG | 0.604022 | 5.1:-41903892 | MS.gene78744:CDS |
| GATTGTGGTAGAAGTGTTGG+TGG | 0.608270 | 5.1:-41903917 | MS.gene78744:CDS |
| GAGGTGATGGAGGTTCATGT+AGG | 0.609166 | 5.1:-41903415 | MS.gene78744:CDS |
| TCTCTCTGCAGTTTCAGAGG+TGG | 0.611498 | 5.1:-41903833 | MS.gene78744:CDS |
| TCTGTTGAGGTAGGAAGTGT+TGG | 0.616434 | 5.1:-41904136 | MS.gene78744:CDS |
| AGAAGTTCAGGTGTGAATGG+TGG | 0.616507 | 5.1:-41903740 | MS.gene78744:CDS |
| TGAAGCTTATAGCAATGACA+TGG | 0.617179 | 5.1:-41904344 | MS.gene78744:CDS |
| GTTCAGATTGTGGAGAACAA+AGG | 0.625847 | 5.1:-41904210 | MS.gene78744:CDS |
| ATTGTGGTAGAAGTGTTGGT+GGG | 0.637779 | 5.1:-41903916 | MS.gene78744:CDS |
| TGAGAAGAATGAGATGTGGA+TGG | 0.655301 | 5.1:-41903888 | MS.gene78744:CDS |
| ATATTTGGATCTGAAGATGG+TGG | 0.728625 | 5.1:-41903806 | MS.gene78744:CDS |
| TGGATGGTTGATCATCATGG+AGG | 0.776999 | 5.1:-41903872 | MS.gene78744:CDS |
| TTCATGACACATGATGCTGG+TGG | 0.799625 | 5.1:-41903449 | MS.gene78744:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTCATCTGAATCAAAAA+TGG | - | chr5.1:41904146-41904165 | MS.gene78744:CDS | 20.0% |
| !! | TTCTTCTATGAATATGAAAA+TGG | - | chr5.1:41904003-41904022 | MS.gene78744:CDS | 20.0% |
| !!! | ATATAAAAAGACATGGTTTT+TGG | - | chr5.1:41903680-41903699 | MS.gene78744:CDS | 20.0% |
| !!! | GAAAGAAGAAAAAGTATTAA+GGG | - | chr5.1:41904297-41904316 | MS.gene78744:CDS | 20.0% |
| ! | ATTTCAGCATTTGAATTCTA+AGG | - | chr5.1:41903591-41903610 | MS.gene78744:CDS | 25.0% |
| ! | TAGAATTCAAATGCTGAAAT+GGG | + | chr5.1:41903591-41903610 | None:intergenic | 25.0% |
| ! | TTAGAATTCAAATGCTGAAA+TGG | + | chr5.1:41903592-41903611 | None:intergenic | 25.0% |
| !! | TCAAATATCATTCAAGTTCA+AGG | - | chr5.1:41904334-41904353 | MS.gene78744:CDS | 25.0% |
| !! | TGTGTTGATATAAAAAGACA+TGG | - | chr5.1:41903673-41903692 | MS.gene78744:CDS | 25.0% |
| !!! | GGAAAGAAGAAAAAGTATTA+AGG | - | chr5.1:41904296-41904315 | MS.gene78744:CDS | 25.0% |
| AATAAGAAGAACAAGGATTG+TGG | - | chr5.1:41903727-41903746 | MS.gene78744:CDS | 30.0% | |
| GAATGAAAGAGGAATTAAGA+GGG | - | chr5.1:41904275-41904294 | MS.gene78744:CDS | 30.0% | |
| TAGAAGAAATCACTTCTTCT+TGG | + | chr5.1:41903991-41904010 | None:intergenic | 30.0% | |
| TAGGAAGAATAAGAAGAACA+AGG | - | chr5.1:41903720-41903739 | MS.gene78744:CDS | 30.0% | |
| TGAATGAAAGAGGAATTAAG+AGG | - | chr5.1:41904274-41904293 | MS.gene78744:CDS | 30.0% | |
| TTACTGTCTTAAAGAACGTT+TGG | - | chr5.1:41903414-41903433 | MS.gene78744:CDS | 30.0% | |
| ! | CTAAGAGAGAGTGATTTTAA+AGG | - | chr5.1:41904087-41904106 | MS.gene78744:CDS | 30.0% |
| !! | GAAGAAAAAGTATTAAGGGT+TGG | - | chr5.1:41904301-41904320 | MS.gene78744:CDS | 30.0% |
| AACAGAGCATGAATTTCGAT+TGG | + | chr5.1:41903509-41903528 | None:intergenic | 35.0% | |
| AATTTCATGACACATGATGC+TGG | - | chr5.1:41904207-41904226 | MS.gene78744:CDS | 35.0% | |
| AGGTTCACTATGAATGAAAG+AGG | - | chr5.1:41904264-41904283 | MS.gene78744:CDS | 35.0% | |
| ATATTTGGATCTGAAGATGG+TGG | - | chr5.1:41903853-41903872 | MS.gene78744:CDS | 35.0% | |
| GCTATATTTGGATCTGAAGA+TGG | - | chr5.1:41903850-41903869 | MS.gene78744:CDS | 35.0% | |
| GGAGAATTGGGAAACTATTT+AGG | - | chr5.1:41903701-41903720 | MS.gene78744:CDS | 35.0% | |
| GTTTGATGTTAGAATCTGGT+AGG | - | chr5.1:41903941-41903960 | MS.gene78744:CDS | 35.0% | |
| TGAAGCTTATAGCAATGACA+TGG | - | chr5.1:41903315-41903334 | MS.gene78744:CDS | 35.0% | |
| TTCTTCTTTCTCTTGCATCT+TGG | + | chr5.1:41903617-41903636 | None:intergenic | 35.0% | |
| TTGATGAGAAGAATGAGATG+TGG | - | chr5.1:41903767-41903786 | MS.gene78744:CDS | 35.0% | |
| ! | AAAGTATTAAGGGTTGGAGA+TGG | - | chr5.1:41904307-41904326 | MS.gene78744:CDS | 35.0% |
| ! | ACTGGTGTAATGGAAATTGA+TGG | - | chr5.1:41904036-41904055 | MS.gene78744:CDS | 35.0% |
| ! | GAAAATGGTAGAAAGAGTAC+TGG | - | chr5.1:41904018-41904037 | MS.gene78744:CDS | 35.0% |
| !! | AGACATGGTTTTTGGAGAAT+TGG | - | chr5.1:41903688-41903707 | MS.gene78744:CDS | 35.0% |
| !! | AGAGAGTGATTTTAAAGGCA+TGG | - | chr5.1:41904092-41904111 | MS.gene78744:CDS | 35.0% |
| !! | GACATGGTTTTTGGAGAATT+GGG | - | chr5.1:41903689-41903708 | MS.gene78744:CDS | 35.0% |
| !! | TAGAAAGAGTACTGGTGTAA+TGG | - | chr5.1:41904026-41904045 | MS.gene78744:CDS | 35.0% |
| AAGCTTGTGTGTTCAGATTG+TGG | - | chr5.1:41903439-41903458 | MS.gene78744:CDS | 40.0% | |
| AATTCATGCTCTGTTGAGGT+AGG | - | chr5.1:41903514-41903533 | MS.gene78744:CDS | 40.0% | |
| ATGTGGATGGTTGATCATCA+TGG | - | chr5.1:41903784-41903803 | MS.gene78744:CDS | 40.0% | |
| ATTTCTGCAGCTAGAAGTTC+AGG | - | chr5.1:41903907-41903926 | MS.gene78744:CDS | 40.0% | |
| CATCCTTCTTCTTCTTCTTC+TGG | - | chr5.1:41903379-41903398 | MS.gene78744:CDS | 40.0% | |
| GAAAGAAGAAGAAGAGGTTG+TGG | - | chr5.1:41903627-41903646 | MS.gene78744:CDS | 40.0% | |
| GAAGAAGGATGCTTCTTACA+TGG | + | chr5.1:41903370-41903389 | None:intergenic | 40.0% | |
| GCAAGAGAAAGAAGAAGAAG+AGG | - | chr5.1:41903621-41903640 | MS.gene78744:CDS | 40.0% | |
| GGTGGTTTGATGTTAGAATC+TGG | - | chr5.1:41903937-41903956 | MS.gene78744:CDS | 40.0% | |
| GTTCAGATTGTGGAGAACAA+AGG | - | chr5.1:41903449-41903468 | MS.gene78744:CDS | 40.0% | |
| TCGAAATTCATGCTCTGTTG+AGG | - | chr5.1:41903510-41903529 | MS.gene78744:CDS | 40.0% | |
| TGAGAAGAATGAGATGTGGA+TGG | - | chr5.1:41903771-41903790 | MS.gene78744:CDS | 40.0% | |
| TTAGAATCTGGTAGGAGAAG+TGG | - | chr5.1:41903949-41903968 | MS.gene78744:CDS | 40.0% | |
| TTCAGAGGTGGAGCTATATT+TGG | - | chr5.1:41903838-41903857 | MS.gene78744:CDS | 40.0% | |
| TTGATGGAAGCAGCTACAAT+AGG | - | chr5.1:41904052-41904071 | MS.gene78744:CDS | 40.0% | |
| ! | AAGGATTGTGGTAGAAGTGT+TGG | - | chr5.1:41903739-41903758 | MS.gene78744:CDS | 40.0% |
| !! | ATCATCATGGAGGTGTTTCA+AGG | - | chr5.1:41903797-41903816 | MS.gene78744:CDS | 40.0% |
| !! | ATTGTGGTAGAAGTGTTGGT+GGG | - | chr5.1:41903743-41903762 | MS.gene78744:CDS | 40.0% |
| !! | GGTGGTGATTCAGTTTTATC+AGG | - | chr5.1:41903871-41903890 | MS.gene78744:CDS | 40.0% |
| !! | TCAGCTTTTGGAAGCATAAG+AGG | - | chr5.1:41904180-41904199 | MS.gene78744:CDS | 40.0% |
| AGAAGTTCAGGTGTGAATGG+TGG | - | chr5.1:41903919-41903938 | MS.gene78744:CDS | 45.0% | |
| AGATCTCTCTGCAGTTTCAG+AGG | - | chr5.1:41903823-41903842 | MS.gene78744:CDS | 45.0% | |
| CCTCCAGAAGAAGAAGAAGA+AGG | + | chr5.1:41903385-41903404 | None:intergenic | 45.0% | |
| CCTTCTTCTTCTTCTTCTGG+AGG | - | chr5.1:41903382-41903401 | MS.gene78744:CDS | 45.0% | |
| GCTAGAAGTTCAGGTGTGAA+TGG | - | chr5.1:41903916-41903935 | MS.gene78744:CDS | 45.0% | |
| TGGATGGTTGATCATCATGG+AGG | - | chr5.1:41903787-41903806 | MS.gene78744:CDS | 45.0% | |
| TTCATGACACATGATGCTGG+TGG | - | chr5.1:41904210-41904229 | MS.gene78744:CDS | 45.0% | |
| ! | GCAGATACACTCTCAGCTTT+TGG | - | chr5.1:41904168-41904187 | MS.gene78744:CDS | 45.0% |
| ! | TCTGTTGAGGTAGGAAGTGT+TGG | - | chr5.1:41903523-41903542 | MS.gene78744:CDS | 45.0% |
| !! | GATTGTGGTAGAAGTGTTGG+TGG | - | chr5.1:41903742-41903761 | MS.gene78744:CDS | 45.0% |
| !! | GCTTTTGGAAGCATAAGAGG+TGG | - | chr5.1:41904183-41904202 | MS.gene78744:CDS | 45.0% |
| ATGACACATGATGCTGGTGG+TGG | - | chr5.1:41904213-41904232 | MS.gene78744:CDS | 50.0% | |
| TCTCTCTGCAGTTTCAGAGG+TGG | - | chr5.1:41903826-41903845 | MS.gene78744:CDS | 50.0% | |
| !! | GAGGTGATGGAGGTTCATGT+AGG | - | chr5.1:41904244-41904263 | MS.gene78744:CDS | 50.0% |
| !! | ACACATGATGCTGGTGGTGG+TGG | - | chr5.1:41904216-41904235 | MS.gene78744:CDS | 55.0% |
| !! | CATGATGCTGGTGGTGGTGG+TGG | - | chr5.1:41904219-41904238 | MS.gene78744:CDS | 60.0% |
| !! | GATGCTGGTGGTGGTGGTGG+TGG | - | chr5.1:41904222-41904241 | MS.gene78744:CDS | 65.0% |
| !! | GGTGGTGGTGGAGGTGATGG+AGG | - | chr5.1:41904234-41904253 | MS.gene78744:CDS | 65.0% |
| !! | GGTGGTGGTGGTGGAGGTGA+TGG | - | chr5.1:41904231-41904250 | MS.gene78744:CDS | 65.0% |
| !! | GCTGGTGGTGGTGGTGGTGG+AGG | - | chr5.1:41904225-41904244 | MS.gene78744:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 41903292 | 41904389 | 41903292 | ID=MS.gene78744 |
| chr5.1 | mRNA | 41903292 | 41904389 | 41903292 | ID=MS.gene78744.t1;Parent=MS.gene78744 |
| chr5.1 | exon | 41903292 | 41904389 | 41903292 | ID=MS.gene78744.t1.exon1;Parent=MS.gene78744.t1 |
| chr5.1 | CDS | 41903292 | 41904389 | 41903292 | ID=cds.MS.gene78744.t1;Parent=MS.gene78744.t1 |
| Gene Sequence |
| Protein sequence |
