AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78886


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78886.t1 MTR_8g041770 57.692 156 19 1 52 160 287 442 1.50e-48 162
MS.gene78886.t1 MTR_8g041770 89.474 57 6 0 2 58 98 154 1.84e-29 112
MS.gene78886.t1 MTR_4g094825 38.272 243 55 4 1 159 160 391 4.15e-46 155
MS.gene78886.t1 MTR_4g022760 64.078 103 35 1 60 160 296 398 3.49e-42 144
MS.gene78886.t1 MTR_4g022760 65.517 58 20 0 1 58 88 145 4.38e-18 80.1
MS.gene78886.t1 MTR_0190s0020 50.331 151 28 1 56 159 292 442 5.67e-39 137
MS.gene78886.t1 MTR_0190s0020 68.966 58 18 0 1 58 97 154 3.70e-21 89.0
MS.gene78886.t1 MTR_4g022800 44.516 155 39 1 53 160 289 443 8.66e-36 129
MS.gene78886.t1 MTR_4g022800 63.793 58 21 0 1 58 88 145 6.42e-17 77.0
MS.gene78886.t1 MTR_4g022825 45.890 146 33 1 60 159 295 440 2.25e-35 127
MS.gene78886.t1 MTR_4g022825 66.667 57 19 0 2 58 89 145 1.26e-19 84.7
MS.gene78886.t1 MTR_4g022670 42.581 155 43 1 52 160 288 442 2.49e-35 127
MS.gene78886.t1 MTR_4g022670 69.091 55 17 0 1 55 88 142 1.68e-17 78.6
MS.gene78886.t1 MTR_7g026860 46.000 150 38 2 54 160 290 439 3.30e-35 127
MS.gene78886.t1 MTR_7g026860 63.793 58 21 0 1 58 88 145 1.58e-17 78.6
MS.gene78886.t1 MTR_4g022720 43.871 155 41 2 52 160 282 436 8.39e-35 126
MS.gene78886.t1 MTR_4g022720 67.241 58 19 0 1 58 88 145 3.94e-19 83.2
MS.gene78886.t1 MTR_3g026310 39.216 153 45 1 56 160 297 449 1.96e-30 114
MS.gene78886.t1 MTR_3g026310 70.175 57 17 0 2 58 89 145 1.37e-21 90.1
MS.gene78886.t1 MTR_8g040780 40.268 149 39 1 56 154 79 227 2.11e-29 108
MS.gene78886.t1 MTR_3g088420 41.497 147 40 1 60 160 288 434 2.47e-29 111
MS.gene78886.t1 MTR_3g088420 75.439 57 14 0 2 58 89 145 8.17e-22 90.9
MS.gene78886.t1 MTR_4g094835 39.333 150 42 2 54 155 294 442 2.19e-26 103
MS.gene78886.t1 MTR_4g094835 65.517 58 20 0 1 58 100 157 4.58e-21 88.6
MS.gene78886.t1 MTR_4g094832 40.278 144 37 2 60 155 284 426 3.69e-26 102
MS.gene78886.t1 MTR_4g094832 67.241 58 19 0 1 58 84 141 1.58e-21 90.1
MS.gene78886.t1 MTR_4g094842 37.931 145 43 2 60 157 302 446 1.27e-24 98.6
MS.gene78886.t1 MTR_4g094842 74.138 58 15 0 1 58 102 159 5.44e-23 94.0
MS.gene78886.t1 MTR_4g094838 38.889 144 39 2 60 155 286 428 1.52e-24 98.2
MS.gene78886.t1 MTR_4g094838 65.517 58 20 0 1 58 102 159 2.86e-20 86.7
MS.gene78886.t1 MTR_7g026780 50.575 87 21 1 96 160 26 112 2.84e-22 86.3
MS.gene78886.t1 MTR_8g040760 71.930 57 16 0 2 58 95 151 3.92e-22 88.6
MS.gene78886.t1 MTR_6g034495 68.421 57 18 0 2 58 71 127 1.96e-21 85.5
MS.gene78886.t1 MTR_6g016815 70.175 57 17 0 2 58 103 159 4.66e-21 88.2
MS.gene78886.t1 MTR_8g079710 36.620 142 39 3 60 150 295 436 2.28e-19 84.0
MS.gene78886.t1 MTR_8g079710 65.517 58 20 0 1 58 93 150 4.85e-19 83.2
MS.gene78886.t1 MTR_7g026850 63.793 58 21 0 1 58 71 128 8.25e-19 79.0
MS.gene78886.t1 MTR_7g026870 57.377 61 26 0 55 115 91 151 1.24e-18 77.8
MS.gene78886.t1 MTR_4g022290 63.793 58 21 0 1 58 94 151 4.06e-18 80.5
MS.gene78886.t1 MTR_4g022290 32.727 165 59 6 42 156 280 442 7.75e-15 71.2
MS.gene78886.t1 MTR_7g026830 60.345 58 23 0 1 58 20 77 1.93e-17 73.2
MS.gene78886.t1 MTR_4g094828 34.667 150 47 5 60 159 302 450 1.07e-16 76.3
MS.gene78886.t1 MTR_4g094828 56.897 58 25 0 1 58 93 150 1.28e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78886.t1 AT5G67160 34.815 135 76 5 17 151 254 376 1.06e-11 62.0

Find 25 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTAATGATCCTGAGTTTA+TGG 0.261735 8.3:+56252415 MS.gene78886:CDS
CCATTGAAAGAGGATTATTT+TGG 0.285934 8.3:+56252016 MS.gene78886:CDS
AGGTGTAGAAGAAGGTAGTA+TGG 0.364829 8.3:+56252352 MS.gene78886:intron
AATTGCCTCAAAAGTTTCAT+AGG 0.367209 8.3:-56252393 None:intergenic
CCAAAATAATCCTCTTTCAA+TGG 0.374799 8.3:-56252016 None:intergenic
TCAATGGAGGAATCAACCTT+TGG 0.403212 8.3:-56252000 None:intergenic
ACACTAGAAGCAACATTAGT+TGG 0.409033 8.3:-56252447 None:intergenic
TGGACTTGGTAAAGGTGCTT+TGG 0.409489 8.3:+56252096 MS.gene78886:CDS
GTTGATTCCTCCATTGAAAG+AGG 0.477667 8.3:+56252006 MS.gene78886:CDS
GTGTTTAAAGGTGTAGAAGA+AGG 0.498123 8.3:+56252344 MS.gene78886:intron
ACCAGTGTCGATGACATCCT+TGG 0.503399 8.3:+56251439 MS.gene78886:CDS
CAAGACAATGGACTTGGTAA+AGG 0.507284 8.3:+56252088 MS.gene78886:CDS
GGTTATAGGCGTTAGACCAA+AGG 0.529274 8.3:+56251984 MS.gene78886:intron
GTTACTTGGACAGCAAGTAA+TGG 0.529511 8.3:-56251538 None:intergenic
TCCGTTGTTAGCATGAAAGT+TGG 0.545219 8.3:+56252055 MS.gene78886:CDS
TAGGATGATATGTGGCTCCA+AGG 0.569745 8.3:-56251456 None:intergenic
TCCAAGGATGTCATCGACAC+TGG 0.591435 8.3:-56251440 None:intergenic
AAATAATCCTCTTTCAATGG+AGG 0.592205 8.3:-56252013 None:intergenic
TTGTTGCAAGACAATGGACT+TGG 0.601437 8.3:+56252082 MS.gene78886:CDS
TCCATCATGTGCAACAATGC+GGG 0.603295 8.3:+56251385 None:intergenic
ATACCAAGAGTAATAAAACT+AGG 0.607573 8.3:-56252190 None:intergenic
GGTGATTTGTTGCAAGACAA+TGG 0.609643 8.3:+56252076 MS.gene78886:CDS
GACACAACATCCATAAACTC+AGG 0.636566 8.3:-56252425 None:intergenic
ACCAACTTTCATGCTAACAA+CGG 0.674514 8.3:-56252056 None:intergenic
TTCCATCATGTGCAACAATG+CGG 0.685385 8.3:+56251384 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGTTTGTGTGTTTAA+AGG + chr8.3:56252332-56252351 MS.gene78886:intron 20.0%
!! TACAATAGTAAAACATTAGT+GGG + chr8.3:56252214-56252233 MS.gene78886:intron 20.0%
!!! AATGTTTATGGTAATGATTT+TGG + chr8.3:56252256-56252275 MS.gene78886:intron 20.0%
!!! TTAGAATGATGATTATTTTG+TGG - chr8.3:56251757-56251776 None:intergenic 20.0%
! ATACCAAGAGTAATAAAACT+AGG - chr8.3:56252193-56252212 None:intergenic 25.0%
! ATTACCATAAACATTAAACC+AGG - chr8.3:56252251-56252270 None:intergenic 25.0%
! ATTGTGAAAGGAAATTGAAT+CGG - chr8.3:56251730-56251749 None:intergenic 25.0%
! GTACAATAGTAAAACATTAG+TGG + chr8.3:56252213-56252232 MS.gene78886:intron 25.0%
! TTACCATAAACATTAAACCA+GGG - chr8.3:56252250-56252269 None:intergenic 25.0%
!! AAAAATGAGTGCAGAATTTT+AGG - chr8.3:56251478-56251497 None:intergenic 25.0%
!!! CACTCATTTTTTCCATTTAA+CGG + chr8.3:56251487-56251506 MS.gene78886:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG + chr8.3:56252261-56252280 MS.gene78886:intron 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG + chr8.3:56252260-56252279 MS.gene78886:intron 25.0%
AAAAACTCAACCTAAATGAG+AGG + chr8.3:56251797-56251816 MS.gene78886:intron 30.0%
AAATAATCCTCTTTCAATGG+AGG - chr8.3:56252016-56252035 None:intergenic 30.0%
AAATTCCATCATTTGAACGT+TGG + chr8.3:56251683-56251702 MS.gene78886:intron 30.0%
AATGAAACATCCTCTCATTT+AGG - chr8.3:56251810-56251829 None:intergenic 30.0%
AATTGCCTCAAAAGTTTCAT+AGG - chr8.3:56252396-56252415 None:intergenic 30.0%
CAAGTAACAGAACTACTTAA+TGG + chr8.3:56251553-56251572 MS.gene78886:intron 30.0%
CCAAAATAATCCTCTTTCAA+TGG - chr8.3:56252019-56252038 None:intergenic 30.0%
GGGAGTGAAAATAATGAAAA+TGG + chr8.3:56252307-56252326 MS.gene78886:intron 30.0%
TGAAAAATGACTATGAGAGT+TGG + chr8.3:56252158-56252177 MS.gene78886:CDS 30.0%
TTGGCATTTCATCAATTCAT+GGG + chr8.3:56251627-56251646 MS.gene78886:intron 30.0%
TTTGGCATTTCATCAATTCA+TGG + chr8.3:56251626-56251645 MS.gene78886:intron 30.0%
! CATTAAGTAGTTCTGTTACT+TGG - chr8.3:56251555-56251574 None:intergenic 30.0%
! CCATTGAAAGAGGATTATTT+TGG + chr8.3:56252016-56252035 MS.gene78886:CDS 30.0%
! TAGTTTTTCACTCCGTTAAA+TGG - chr8.3:56251502-56251521 None:intergenic 30.0%
! TTTGTCCTATGAAACTTTTG+AGG + chr8.3:56252388-56252407 MS.gene78886:CDS 30.0%
!! AAGACAACCATTTGAGTTTT+TGG - chr8.3:56251653-56251672 None:intergenic 30.0%
!! ACGTTCAAATGATGGAATTT+TGG - chr8.3:56251683-56251702 None:intergenic 30.0%
!! TTTTGTGGTTCTATTGTGAA+AGG - chr8.3:56251742-56251761 None:intergenic 30.0%
!!! ACACCTAGTTTTATTACTCT+TGG + chr8.3:56252187-56252206 MS.gene78886:CDS 30.0%
!!! CAGAATTTTAGGATGATATG+TGG - chr8.3:56251467-56251486 None:intergenic 30.0%
!!! TATGAAACTTTTGAGGCAAT+TGG + chr8.3:56252395-56252414 MS.gene78886:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG + chr8.3:56252262-56252281 MS.gene78886:intron 30.0%
ACCAACTTTCATGCTAACAA+CGG - chr8.3:56252059-56252078 None:intergenic 35.0%
TCACCCTGGTTTAATGTTTA+TGG + chr8.3:56252244-56252263 MS.gene78886:intron 35.0%
! AAGCACTTTTCACTCACATT+TGG + chr8.3:56251905-56251924 MS.gene78886:intron 35.0%
! GTGTTTAAAGGTGTAGAAGA+AGG + chr8.3:56252344-56252363 MS.gene78886:intron 35.0%
! TGGTAATGATCCTGAGTTTA+TGG + chr8.3:56252415-56252434 MS.gene78886:CDS 35.0%
AACGGAGTGAAAAACTACGA+AGG + chr8.3:56251505-56251524 MS.gene78886:CDS 40.0%
ACATAATGCACTTCTTCTCG+AGG - chr8.3:56251961-56251980 None:intergenic 40.0%
ACGGAGTGAAAAACTACGAA+GGG + chr8.3:56251506-56251525 MS.gene78886:CDS 40.0%
AGGTGTAGAAGAAGGTAGTA+TGG + chr8.3:56252352-56252371 MS.gene78886:intron 40.0%
CATAATGCACTTCTTCTCGA+GGG - chr8.3:56251960-56251979 None:intergenic 40.0%
CTTTGTACCAGCTTCTAAGT+TGG - chr8.3:56251869-56251888 None:intergenic 40.0%
GACACAACATCCATAAACTC+AGG - chr8.3:56252428-56252447 None:intergenic 40.0%
GGAAACCAACGTTCAAATGA+TGG - chr8.3:56251691-56251710 None:intergenic 40.0%
GTGAAAGGAAATTGAATCGG+AGG - chr8.3:56251727-56251746 None:intergenic 40.0%
GTGCATTATGTGGTGGTTAT+AGG + chr8.3:56251970-56251989 MS.gene78886:intron 40.0%
GTTACTTGGACAGCAAGTAA+TGG - chr8.3:56251541-56251560 None:intergenic 40.0%
TCAATGGAGGAATCAACCTT+TGG - chr8.3:56252003-56252022 None:intergenic 40.0%
TCATGGGCCAAAAACTCAAA+TGG + chr8.3:56251643-56251662 MS.gene78886:intron 40.0%
TCCGTTGTTAGCATGAAAGT+TGG + chr8.3:56252055-56252074 MS.gene78886:CDS 40.0%
TTCAAAGCCAACTTAGAAGC+TGG + chr8.3:56251859-56251878 MS.gene78886:intron 40.0%
! AGAAGAAGTGCATTATGTGG+TGG + chr8.3:56251963-56251982 MS.gene78886:intron 40.0%
! CAAGACAATGGACTTGGTAA+AGG + chr8.3:56252088-56252107 MS.gene78886:CDS 40.0%
! GTTGATTCCTCCATTGAAAG+AGG + chr8.3:56252006-56252025 MS.gene78886:CDS 40.0%
! TCGAGAAGAAGTGCATTATG+TGG + chr8.3:56251960-56251979 MS.gene78886:intron 40.0%
! TTGTTGCAAGACAATGGACT+TGG + chr8.3:56252082-56252101 MS.gene78886:CDS 40.0%
!! GGTGATTTGTTGCAAGACAA+TGG + chr8.3:56252076-56252095 MS.gene78886:CDS 40.0%
!! TTTGAACGTTGGTTTCCAGA+TGG + chr8.3:56251694-56251713 MS.gene78886:intron 40.0%
ATCGGAGGTTGAATACCATC+TGG - chr8.3:56251712-56251731 None:intergenic 45.0%
GGTTATAGGCGTTAGACCAA+AGG + chr8.3:56251984-56252003 MS.gene78886:intron 45.0%
GTTAATCATGTGGTCGCTGA+TGG + chr8.3:56251595-56251614 MS.gene78886:intron 45.0%
TAGGATGATATGTGGCTCCA+AGG - chr8.3:56251459-56251478 None:intergenic 45.0%
TGCGTGCACAGTTAATCATG+TGG + chr8.3:56251585-56251604 MS.gene78886:intron 45.0%
TTTGGTTGGGGAAAACCTGT+GGG + chr8.3:56252274-56252293 MS.gene78886:intron 45.0%
! TTCTCGAGGGTCAAGGTTTT+TGG - chr8.3:56251947-56251966 None:intergenic 45.0%
!! TGGACTTGGTAAAGGTGCTT+TGG + chr8.3:56252096-56252115 MS.gene78886:CDS 45.0%
!! TTTTGGTTGGGGAAAACCTG+TGG + chr8.3:56252273-56252292 MS.gene78886:intron 45.0%
TCCAAGGATGTCATCGACAC+TGG - chr8.3:56251443-56251462 None:intergenic 50.0%
TTGGTTGGGGAAAACCTGTG+GGG + chr8.3:56252275-56252294 MS.gene78886:intron 50.0%
! TAGTGGGTAGTAGTTCACCC+TGG + chr8.3:56252230-56252249 MS.gene78886:intron 50.0%
! TCGCTGATGGCACATCGATT+TGG + chr8.3:56251608-56251627 MS.gene78886:intron 50.0%
!! ACCAGTGTCGATGACATCCT+TGG + chr8.3:56251439-56251458 MS.gene78886:CDS 50.0%
AAACCTGTGGGGGTTCGAAC+TGG + chr8.3:56252286-56252305 MS.gene78886:intron 55.0%
AACCTGTGGGGGTTCGAACT+GGG + chr8.3:56252287-56252306 MS.gene78886:intron 55.0%
GCACTTCTTCTCGAGGGTCA+AGG - chr8.3:56251954-56251973 None:intergenic 55.0%
TGGTTGGGGAAAACCTGTGG+GGG + chr8.3:56252276-56252295 MS.gene78886:intron 55.0%
CTCCCAGTTCGAACCCCCAC+AGG - chr8.3:56252292-56252311 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 56251391 56252454 56251391 ID=MS.gene78886
chr8.3 mRNA 56251391 56252454 56251391 ID=MS.gene78886.t1;Parent=MS.gene78886
chr8.3 exon 56251391 56251555 56251391 ID=MS.gene78886.t1.exon1;Parent=MS.gene78886.t1
chr8.3 CDS 56251391 56251555 56251391 ID=cds.MS.gene78886.t1;Parent=MS.gene78886.t1
chr8.3 exon 56251992 56252208 56251992 ID=MS.gene78886.t1.exon2;Parent=MS.gene78886.t1
chr8.3 CDS 56251992 56252208 56251992 ID=cds.MS.gene78886.t1;Parent=MS.gene78886.t1
chr8.3 exon 56252354 56252454 56252354 ID=MS.gene78886.t1.exon3;Parent=MS.gene78886.t1
chr8.3 CDS 56252354 56252454 56252354 ID=cds.MS.gene78886.t1;Parent=MS.gene78886.t1
Gene Sequence

>MS.gene78886

ATGTGCAACAATGCGGGTGCACTCTTTGTTCATGCTGCAGCAGAAAATACCAGTGTCGATGACATCCTTGGAGCCACATATCATCCTAAAATTCTGCACTCATTTTTTCCATTTAACGGAGTGAAAAACTACGAAGGGACATCAAAGCCATTACTTGCTGTCCAAGTAACAGAACTACTTAATGGCATCTTCATTGCGTGCACAGTTAATCATGTGGTCGCTGATGGCACATCGATTTGGCATTTCATCAATTCATGGGCCAAAAACTCAAATGGTTGTCTTGAAATATCCAAAATTCCATCATTTGAACGTTGGTTTCCAGATGGTATTCAACCTCCGATTCAATTTCCTTTCACAATAGAACCACAAAATAATCATCATTCTAATAATCATTATCATAAAGAAGAAAAACTCAACCTAAATGAGAGGATGTTTCATTTCACAAAAGAGAATATTGCAAAACTAAAATTCAAAGCCAACTTAGAAGCTGGTACAAAGAACATATCTTCTTTGCAAGCACTTTTCACTCACATTTGGCGATCTATTATACGTTCCAAAAACCTTGACCCTCGAGAAGAAGTGCATTATGTGGTGGTTATAGGCGTTAGACCAAAGGTTGATTCCTCCATTGAAAGAGGATTATTTTGGTAACGCTATGATAGACTCCGTTGTTAGCATGAAAGTTGGTGATTTGTTGCAAGACAATGGACTTGGTAAAGGTGCTTTGGAGATGAACAAGATGATTGCTTTGCAATCTGATGAGATGTTGAAAAATGACTATGAGAGTTGGTTGAAAACACCTAGTTTTATTACTCTTGGTATGTACAATAGTAAAACATTAGTGGGTAGTAGTTCACCCTGGTTTAATGTTTATGGTAATGATTTTGGTTGGGGAAAACCTGTGGGGGTTCGAACTGGGAGTGAAAATAATGAAAATGGAAATATTTGTTTGTGTGTTTAAAGGTGTAGAAGAAGGTAGTATGGATCTTGAAGTGTGTTTGTCCTATGAAACTTTTGAGGCAATTGGTAATGATCCTGAGTTTATGGATGTTGTGTCCAACTAA

Protein sequence

>MS.gene78886.t1

MCNNAGALFVHAAAENTSVDDILGATYHPKILHSFFPFNGVKNYEGTSKPLLAVQALDQRLIPPLKEDYFGNAMIDSVVSMKVGDLLQDNGLGKGALEMNKMIALQSDEMLKNDYESWLKTPSFITLGVEEGSMDLEVCLSYETFEAIGNDPEFMDVVSN