Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79442.t1 | XP_003626244.1 | 92.9 | 156 | 11 | 0 | 1 | 156 | 1 | 156 | 8.60E-83 | 316.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79442.t1 | I6YGS0 | 41.4 | 152 | 86 | 3 | 7 | 156 | 7 | 157 | 8.5e-31 | 134.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79442.t1 | A2Q321 | 92.9 | 156 | 11 | 0 | 1 | 156 | 1 | 156 | 6.2e-83 | 316.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054290 | MS.gene79442 | 0.801073 | 1.07E-48 | -1.69E-46 |
| MS.gene057544 | MS.gene79442 | 0.825753 | 3.95E-54 | -1.69E-46 |
| MS.gene058218 | MS.gene79442 | 0.818558 | 1.84E-52 | -1.69E-46 |
| MS.gene059402 | MS.gene79442 | 0.801457 | 8.92E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79442.t1 | MTR_7g113030 | 92.949 | 156 | 11 | 0 | 1 | 156 | 1 | 156 | 1.22e-108 | 312 |
| MS.gene79442.t1 | MTR_7g113050 | 91.667 | 156 | 13 | 0 | 1 | 156 | 1 | 156 | 1.21e-106 | 304 |
| MS.gene79442.t1 | MTR_7g113050 | 91.667 | 156 | 13 | 0 | 1 | 156 | 1 | 156 | 7.45e-106 | 305 |
| MS.gene79442.t1 | MTR_7g112615 | 87.342 | 158 | 20 | 0 | 1 | 158 | 1 | 158 | 6.90e-103 | 292 |
| MS.gene79442.t1 | MTR_7g112615 | 87.821 | 156 | 19 | 0 | 1 | 156 | 1 | 156 | 1.01e-102 | 294 |
| MS.gene79442.t1 | MTR_7g112615 | 87.821 | 156 | 19 | 0 | 1 | 156 | 1 | 156 | 7.99e-102 | 295 |
| MS.gene79442.t1 | MTR_7g112960 | 85.256 | 156 | 23 | 0 | 1 | 156 | 1 | 156 | 1.18e-98 | 287 |
| MS.gene79442.t1 | MTR_7g112970 | 82.911 | 158 | 27 | 0 | 1 | 158 | 1 | 158 | 3.38e-98 | 286 |
| MS.gene79442.t1 | MTR_7g113020 | 83.974 | 156 | 25 | 0 | 1 | 156 | 1 | 156 | 1.63e-97 | 284 |
| MS.gene79442.t1 | MTR_7g112963 | 84.615 | 156 | 24 | 0 | 1 | 156 | 1 | 156 | 2.00e-97 | 284 |
| MS.gene79442.t1 | MTR_7g112620 | 84.615 | 156 | 24 | 0 | 1 | 156 | 1 | 156 | 5.24e-97 | 283 |
| MS.gene79442.t1 | MTR_7g113010 | 83.333 | 156 | 26 | 0 | 1 | 156 | 1 | 156 | 6.14e-95 | 286 |
| MS.gene79442.t1 | MTR_1g083480 | 73.585 | 159 | 38 | 2 | 1 | 156 | 1 | 158 | 5.89e-87 | 252 |
| MS.gene79442.t1 | MTR_4g005160 | 72.956 | 159 | 39 | 2 | 1 | 156 | 1 | 158 | 2.19e-85 | 253 |
| MS.gene79442.t1 | MTR_7g108825 | 66.026 | 156 | 52 | 1 | 1 | 156 | 1 | 155 | 1.12e-69 | 211 |
| MS.gene79442.t1 | MTR_4g120240 | 63.924 | 158 | 54 | 2 | 1 | 156 | 37 | 193 | 1.51e-69 | 214 |
| MS.gene79442.t1 | MTR_7g108825 | 66.026 | 156 | 52 | 1 | 1 | 156 | 1 | 155 | 1.23e-68 | 211 |
| MS.gene79442.t1 | MTR_3g024250 | 90.654 | 107 | 10 | 0 | 46 | 152 | 1 | 107 | 3.64e-68 | 203 |
| MS.gene79442.t1 | MTR_8g076680 | 53.205 | 156 | 72 | 1 | 1 | 156 | 3 | 157 | 5.32e-61 | 188 |
| MS.gene79442.t1 | MTR_8g076680 | 53.205 | 156 | 72 | 1 | 1 | 156 | 3 | 157 | 3.62e-60 | 189 |
| MS.gene79442.t1 | MTR_4g089060 | 39.103 | 156 | 90 | 1 | 1 | 156 | 1 | 151 | 2.75e-38 | 130 |
| MS.gene79442.t1 | MTR_4g089060 | 40.373 | 161 | 90 | 2 | 1 | 160 | 1 | 156 | 3.37e-38 | 133 |
| MS.gene79442.t1 | MTR_4g089060 | 39.103 | 156 | 90 | 1 | 1 | 156 | 1 | 151 | 9.34e-38 | 131 |
| MS.gene79442.t1 | MTR_4g089040 | 39.103 | 156 | 90 | 1 | 1 | 156 | 1 | 151 | 9.37e-36 | 126 |
| MS.gene79442.t1 | MTR_2g090240 | 37.821 | 156 | 93 | 1 | 1 | 156 | 1 | 152 | 7.53e-35 | 124 |
| MS.gene79442.t1 | MTR_2g090245 | 37.179 | 156 | 94 | 2 | 1 | 156 | 1 | 152 | 2.85e-33 | 119 |
| MS.gene79442.t1 | MTR_7g112910 | 51.765 | 85 | 25 | 1 | 72 | 156 | 1 | 69 | 7.82e-24 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79442.t1 | AT3G05600 | 69.811 | 159 | 45 | 2 | 1 | 156 | 1 | 159 | 1.28e-79 | 236 |
| MS.gene79442.t1 | AT3G05600 | 69.811 | 159 | 45 | 2 | 1 | 156 | 1 | 159 | 9.28e-79 | 237 |
| MS.gene79442.t1 | AT4G02340 | 63.462 | 156 | 56 | 1 | 1 | 156 | 1 | 155 | 7.34e-72 | 219 |
| MS.gene79442.t1 | AT2G26740 | 62.893 | 159 | 53 | 3 | 4 | 156 | 1 | 159 | 6.92e-67 | 206 |
| MS.gene79442.t1 | AT4G15955 | 59.259 | 162 | 56 | 2 | 5 | 156 | 6 | 167 | 2.18e-66 | 200 |
| MS.gene79442.t1 | AT4G15955 | 59.259 | 162 | 56 | 2 | 5 | 156 | 6 | 167 | 2.79e-66 | 200 |
| MS.gene79442.t1 | AT4G15955 | 59.146 | 164 | 57 | 2 | 5 | 158 | 6 | 169 | 3.00e-65 | 201 |
| MS.gene79442.t1 | AT2G26750 | 62.025 | 158 | 55 | 3 | 4 | 156 | 1 | 158 | 1.48e-64 | 200 |
| MS.gene79442.t1 | AT4G15960 | 56.173 | 162 | 65 | 2 | 1 | 156 | 51 | 212 | 1.23e-61 | 194 |
| MS.gene79442.t1 | AT3G51000 | 55.195 | 154 | 67 | 2 | 4 | 156 | 5 | 157 | 1.79e-57 | 182 |
Find 41 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGTTTCTTCATGGCTTCC+TGG | 0.181967 | 3.1:-15578491 | MS.gene79442:CDS |
| CCAATTGTCTTGTTTCTTCA+TGG | 0.206846 | 3.1:-15578499 | MS.gene79442:CDS |
| TTATTATATATGTAGATTTC+AGG | 0.260713 | 3.1:-15578128 | MS.gene79442:CDS |
| AAAATGCATATTGCTGAAAA+AGG | 0.266560 | 3.1:-15578532 | MS.gene79442:CDS |
| TTCATGGCTTCCTGGAACTC+TGG | 0.312133 | 3.1:-15578483 | MS.gene79442:CDS |
| TGTTTCTTGTGGCTCATGAT+TGG | 0.349713 | 3.1:-15578288 | MS.gene79442:CDS |
| GTTTCTTGTGGCTCATGATT+GGG | 0.353911 | 3.1:-15578287 | MS.gene79442:CDS |
| CATGATTGGGGTGCTATCAT+TGG | 0.366163 | 3.1:-15578274 | MS.gene79442:CDS |
| TCCTGGAACTCTGGTATTCA+TGG | 0.376261 | 3.1:-15578474 | MS.gene79442:CDS |
| GATGCCATGCATGCTGCTTA+TGG | 0.401538 | 3.1:-15578157 | MS.gene79442:CDS |
| GCCATGAATACCAGAGTTCC+AGG | 0.422356 | 3.1:+15578473 | None:intergenic |
| TCACCATAGCCACGAAGATC+AGG | 0.444071 | 3.1:+15578403 | None:intergenic |
| CAGCTACACAGGGTTTCATA+TGG | 0.449902 | 3.1:-15578356 | MS.gene79442:CDS |
| CCATGAAGAAACAAGACAAT+TGG | 0.459751 | 3.1:+15578499 | None:intergenic |
| GCTCCTGATCTTCGTGGCTA+TGG | 0.464887 | 3.1:-15578406 | MS.gene79442:CDS |
| TACACAGGGTTTCATATGGT+TGG | 0.511886 | 3.1:-15578352 | MS.gene79442:CDS |
| CACAGAACAGTGGAAGTGAA+TGG | 0.515742 | 3.1:-15578559 | MS.gene79442:CDS |
| GTGTAGCTGCTTATTGAAGA+TGG | 0.516512 | 3.1:+15578370 | None:intergenic |
| TCTTCAATAAGCAGCTACAC+AGG | 0.519820 | 3.1:-15578367 | MS.gene79442:CDS |
| GGGAGCTAAGAGCTACAATC+TGG | 0.528583 | 3.1:+15578446 | None:intergenic |
| TTTAGGGTTTCTTCGGAGCA+AGG | 0.536936 | 3.1:+15578192 | None:intergenic |
| GAGCCACGGCATGGTAGCCG+AGG | 0.537610 | 3.1:+15578425 | None:intergenic |
| TTTCTTGTGGCTCATGATTG+GGG | 0.538324 | 3.1:-15578286 | MS.gene79442:CDS |
| GCTTATTGAAGATGGAGAAT+CGG | 0.544508 | 3.1:+15578378 | None:intergenic |
| ATCTCCATAAGCAGCATGCA+TGG | 0.554133 | 3.1:+15578153 | None:intergenic |
| ATTGTAGCTCTTAGCTCCCT+CGG | 0.557522 | 3.1:-15578442 | MS.gene79442:CDS |
| CTCCCTCGGCTACCATGCCG+TGG | 0.565985 | 3.1:-15578428 | MS.gene79442:CDS |
| ATTGGGGTGCTATCATTGGT+TGG | 0.568173 | 3.1:-15578270 | MS.gene79442:CDS |
| GCCGTGGCTCCTGATCTTCG+TGG | 0.568982 | 3.1:-15578412 | MS.gene79442:CDS |
| TTAGGGTTTCTTCGGAGCAA+GGG | 0.576719 | 3.1:+15578193 | None:intergenic |
| CTTCAATAAGCAGCTACACA+GGG | 0.595237 | 3.1:-15578366 | MS.gene79442:CDS |
| GAAGATCAGGAGCCACGGCA+TGG | 0.605772 | 3.1:+15578416 | None:intergenic |
| AGCCACGGCATGGTAGCCGA+GGG | 0.617386 | 3.1:+15578426 | None:intergenic |
| GGGAACACTGAGACAAACAT+AGG | 0.620100 | 3.1:+15578213 | None:intergenic |
| GCCACGAAGATCAGGAGCCA+CGG | 0.626919 | 3.1:+15578411 | None:intergenic |
| AGCTAAGAGCTACAATCTGG+TGG | 0.642643 | 3.1:+15578449 | None:intergenic |
| TGTTGATCAAGTGTTTCTTG+TGG | 0.646029 | 3.1:-15578299 | MS.gene79442:CDS |
| TGTCGGGGCGAAACATGCAA+AGG | 0.661179 | 3.1:+15578245 | None:intergenic |
| AGGAATAGAACACAGAACAG+TGG | 0.676997 | 3.1:-15578569 | MS.gene79442:CDS |
| GTTACACTGAGAGAGATGGA+AGG | 0.677077 | 3.1:-15578589 | None:intergenic |
| GAAAATCAATAAGTGCAACA+AGG | 0.701740 | 3.1:+15578326 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTATATATGTAGATTTC+AGG | - | chr3.1:15578557-15578576 | MS.gene79442:CDS | 15.0% |
| ! | AAAATGCATATTGCTGAAAA+AGG | - | chr3.1:15578153-15578172 | MS.gene79442:CDS | 25.0% |
| ATTGCTGAAAAAGGAAAACA+AGG | - | chr3.1:15578162-15578181 | MS.gene79442:CDS | 30.0% | |
| GAAAATCAATAAGTGCAACA+AGG | + | chr3.1:15578362-15578381 | None:intergenic | 30.0% | |
| ! | CATCAACTGTCTTGATTTTA+GGG | + | chr3.1:15578512-15578531 | None:intergenic | 30.0% |
| !! | AACATAGGCTTTGATTTTGT+CGG | + | chr3.1:15578460-15578479 | None:intergenic | 30.0% |
| !!! | GTTGCACTTATTGATTTTCT+GGG | - | chr3.1:15578363-15578382 | MS.gene79442:CDS | 30.0% |
| !!! | TCTTGATTTTAGGGTTTCTT+CGG | + | chr3.1:15578503-15578522 | None:intergenic | 30.0% |
| !!! | TGTTGCACTTATTGATTTTC+TGG | - | chr3.1:15578362-15578381 | MS.gene79442:CDS | 30.0% |
| CCATGAAGAAACAAGACAAT+TGG | + | chr3.1:15578189-15578208 | None:intergenic | 35.0% | |
| GCTTATTGAAGATGGAGAAT+CGG | + | chr3.1:15578310-15578329 | None:intergenic | 35.0% | |
| TGTTGATCAAGTGTTTCTTG+TGG | - | chr3.1:15578386-15578405 | MS.gene79442:CDS | 35.0% | |
| ! | GCATCAACTGTCTTGATTTT+AGG | + | chr3.1:15578513-15578532 | None:intergenic | 35.0% |
| !! | ACATAGGCTTTGATTTTGTC+GGG | + | chr3.1:15578459-15578478 | None:intergenic | 35.0% |
| !! | CCAATTGTCTTGTTTCTTCA+TGG | - | chr3.1:15578186-15578205 | MS.gene79442:CDS | 35.0% |
| AGGAATAGAACACAGAACAG+TGG | - | chr3.1:15578116-15578135 | MS.gene79442:CDS | 40.0% | |
| CTTCAATAAGCAGCTACACA+GGG | - | chr3.1:15578319-15578338 | MS.gene79442:CDS | 40.0% | |
| GTGTAGCTGCTTATTGAAGA+TGG | + | chr3.1:15578318-15578337 | None:intergenic | 40.0% | |
| GTTTCTTGTGGCTCATGATT+GGG | - | chr3.1:15578398-15578417 | MS.gene79442:CDS | 40.0% | |
| TACACAGGGTTTCATATGGT+TGG | - | chr3.1:15578333-15578352 | MS.gene79442:CDS | 40.0% | |
| TCTTCAATAAGCAGCTACAC+AGG | - | chr3.1:15578318-15578337 | MS.gene79442:CDS | 40.0% | |
| TGTTTCTTGTGGCTCATGAT+TGG | - | chr3.1:15578397-15578416 | MS.gene79442:CDS | 40.0% | |
| TTTCTTGTGGCTCATGATTG+GGG | - | chr3.1:15578399-15578418 | MS.gene79442:CDS | 40.0% | |
| !! | CATAGGCTTTGATTTTGTCG+GGG | + | chr3.1:15578458-15578477 | None:intergenic | 40.0% |
| AGCTAAGAGCTACAATCTGG+TGG | + | chr3.1:15578239-15578258 | None:intergenic | 45.0% | |
| ATCTCCATAAGCAGCATGCA+TGG | + | chr3.1:15578535-15578554 | None:intergenic | 45.0% | |
| ATTGTAGCTCTTAGCTCCCT+CGG | - | chr3.1:15578243-15578262 | MS.gene79442:CDS | 45.0% | |
| CACAGAACAGTGGAAGTGAA+TGG | - | chr3.1:15578126-15578145 | MS.gene79442:CDS | 45.0% | |
| CAGCTACACAGGGTTTCATA+TGG | - | chr3.1:15578329-15578348 | MS.gene79442:CDS | 45.0% | |
| GGGAACACTGAGACAAACAT+AGG | + | chr3.1:15578475-15578494 | None:intergenic | 45.0% | |
| TCCTGGAACTCTGGTATTCA+TGG | - | chr3.1:15578211-15578230 | MS.gene79442:CDS | 45.0% | |
| TTAGGGTTTCTTCGGAGCAA+GGG | + | chr3.1:15578495-15578514 | None:intergenic | 45.0% | |
| ! | ATTGGGGTGCTATCATTGGT+TGG | - | chr3.1:15578415-15578434 | MS.gene79442:CDS | 45.0% |
| ! | CATGATTGGGGTGCTATCAT+TGG | - | chr3.1:15578411-15578430 | MS.gene79442:CDS | 45.0% |
| ! | CTTGTTTCTTCATGGCTTCC+TGG | - | chr3.1:15578194-15578213 | MS.gene79442:CDS | 45.0% |
| ! | TTTAGGGTTTCTTCGGAGCA+AGG | + | chr3.1:15578496-15578515 | None:intergenic | 45.0% |
| GCCATGAATACCAGAGTTCC+AGG | + | chr3.1:15578215-15578234 | None:intergenic | 50.0% | |
| GGGAGCTAAGAGCTACAATC+TGG | + | chr3.1:15578242-15578261 | None:intergenic | 50.0% | |
| TCACCATAGCCACGAAGATC+AGG | + | chr3.1:15578285-15578304 | None:intergenic | 50.0% | |
| TTCATGGCTTCCTGGAACTC+TGG | - | chr3.1:15578202-15578221 | MS.gene79442:CDS | 50.0% | |
| ! | GATGCCATGCATGCTGCTTA+TGG | - | chr3.1:15578528-15578547 | MS.gene79442:CDS | 50.0% |
| GCTCCTGATCTTCGTGGCTA+TGG | - | chr3.1:15578279-15578298 | MS.gene79442:CDS | 55.0% | |
| TGTCGGGGCGAAACATGCAA+AGG | + | chr3.1:15578443-15578462 | None:intergenic | 55.0% | |
| GAAGATCAGGAGCCACGGCA+TGG | + | chr3.1:15578272-15578291 | None:intergenic | 60.0% | |
| GCCACGAAGATCAGGAGCCA+CGG | + | chr3.1:15578277-15578296 | None:intergenic | 60.0% | |
| AGCCACGGCATGGTAGCCGA+GGG | + | chr3.1:15578262-15578281 | None:intergenic | 65.0% | |
| GCCGTGGCTCCTGATCTTCG+TGG | - | chr3.1:15578273-15578292 | MS.gene79442:CDS | 65.0% | |
| CTCCCTCGGCTACCATGCCG+TGG | - | chr3.1:15578257-15578276 | MS.gene79442:CDS | 70.0% | |
| GAGCCACGGCATGGTAGCCG+AGG | + | chr3.1:15578263-15578282 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 15578111 | 15578596 | 15578111 | ID=MS.gene79442 |
| chr3.1 | mRNA | 15578111 | 15578596 | 15578111 | ID=MS.gene79442.t1;Parent=MS.gene79442 |
| chr3.1 | exon | 15578111 | 15578596 | 15578111 | ID=MS.gene79442.t1.exon1;Parent=MS.gene79442.t1 |
| chr3.1 | CDS | 15578111 | 15578596 | 15578111 | ID=cds.MS.gene79442.t1;Parent=MS.gene79442.t1 |
| Gene Sequence |
| Protein sequence |
