Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80684.t1 | XP_003616621.1 | 99.3 | 144 | 1 | 0 | 1 | 144 | 1 | 144 | 8.80E-79 | 302.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80684.t1 | Q9ST43 | 70.2 | 141 | 40 | 1 | 4 | 142 | 3 | 143 | 1.2e-55 | 217.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80684.t1 | G7KGZ7 | 99.3 | 144 | 1 | 0 | 1 | 144 | 1 | 144 | 6.3e-79 | 302.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene013786 | MS.gene80684 | PPI |
| MS.gene80684 | MS.gene46264 | PPI |
| MS.gene80684 | MS.gene02572 | PPI |
| MS.gene80684 | MS.gene03201 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80684.t1 | MTR_5g082470 | 99.306 | 144 | 1 | 0 | 1 | 144 | 1 | 144 | 1.12e-105 | 297 |
| MS.gene80684.t1 | MTR_3g463520 | 72.340 | 141 | 37 | 1 | 4 | 142 | 3 | 143 | 6.84e-75 | 220 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80684.t1 | AT5G05710 | 80.142 | 141 | 27 | 1 | 3 | 142 | 2 | 142 | 9.42e-81 | 234 |
| MS.gene80684.t1 | AT2G29700 | 70.213 | 141 | 40 | 1 | 4 | 142 | 3 | 143 | 1.31e-71 | 211 |
Find 30 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTAACCAGCCGGTTCGTTC+TGG | 0.208272 | 5.4:+64959160 | None:intergenic |
| ACTACTCCGGCGTCGAATTC+TGG | 0.239180 | 5.4:-64959189 | MS.gene80684:CDS |
| GCTACTTGTCTCACTGTTAA+AGG | 0.239265 | 5.4:-64959013 | MS.gene80684:CDS |
| TGAAACAAGGGAAACTCTTC+TGG | 0.272043 | 5.4:-64959090 | MS.gene80684:CDS |
| TTAACCAGCCGGTTCGTTCT+GGG | 0.377723 | 5.4:+64959161 | None:intergenic |
| GAAATTGTTGATTATGATAA+CGG | 0.378590 | 5.4:-64958836 | MS.gene80684:CDS |
| TTCTAGATCTGTTACTGATT+CGG | 0.390424 | 5.4:-64958858 | MS.gene80684:CDS |
| GACCGGGTTTGTGGTTGAAC+CGG | 0.413116 | 5.4:+64959213 | None:intergenic |
| ACAGTGAGACAAGTAGCAAC+AGG | 0.427222 | 5.4:+64959019 | None:intergenic |
| AGGATTGGATTAACTCGATT+GGG | 0.440853 | 5.4:-64958898 | MS.gene80684:CDS |
| GAGGATTGGATTAACTCGAT+TGG | 0.449435 | 5.4:-64958899 | MS.gene80684:CDS |
| AGTCTGTGGCGCTACGCAAC+CGG | 0.467927 | 5.4:-64959232 | MS.gene80684:CDS |
| TGATTCTGATAAGGAGAAAG+AGG | 0.505978 | 5.4:-64958918 | MS.gene80684:CDS |
| CTGATAAGGAGAAAGAGGAT+TGG | 0.515649 | 5.4:-64958913 | MS.gene80684:CDS |
| TGGTCGAACCCAGAACGAAC+CGG | 0.518095 | 5.4:-64959169 | MS.gene80684:CDS |
| GGAGTAGTCGACCGGGTTTG+TGG | 0.532363 | 5.4:+64959204 | None:intergenic |
| ACAAACCCGGTCGACTACTC+CGG | 0.537859 | 5.4:-64959202 | MS.gene80684:CDS |
| CCGGAATGGCAGCGAGTCTG+TGG | 0.538111 | 5.4:-64959246 | None:intergenic |
| TCGACGCCGGAGTAGTCGAC+CGG | 0.566459 | 5.4:+64959196 | None:intergenic |
| AACCGGTTCAACCACAAACC+CGG | 0.568891 | 5.4:-64959215 | MS.gene80684:CDS |
| GTGGAAAGTTCAAACGCGTA+GGG | 0.587874 | 5.4:+64958968 | None:intergenic |
| ATACATCGTATCAGCGCGTG+TGG | 0.592591 | 5.4:+64958949 | None:intergenic |
| TGTGGAAAGTTCAAACGCGT+AGG | 0.611943 | 5.4:+64958967 | None:intergenic |
| CGAACCCAGAACGAACCGGC+TGG | 0.620445 | 5.4:-64959165 | MS.gene80684:CDS |
| CGACGCCGGAGTAGTCGACC+GGG | 0.622012 | 5.4:+64959197 | None:intergenic |
| ACCGGCTGGTTAACAAAACA+AGG | 0.628016 | 5.4:-64959151 | MS.gene80684:CDS |
| CAGTGAGACAAGTAGCAACA+GGG | 0.674850 | 5.4:+64959020 | None:intergenic |
| GTTCGACCAGAATTCGACGC+CGG | 0.725090 | 5.4:+64959183 | None:intergenic |
| ACACGCGCTTCTATACCGCG+TGG | 0.800840 | 5.4:-64959049 | MS.gene80684:CDS |
| GCAACAGGGATAACACCACG+CGG | 0.840792 | 5.4:+64959034 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTGTTGATTATGATAA+CGG | - | chr5.4:64959234-64959253 | MS.gene80684:CDS | 20.0% |
| !!! | TTTTATTGCTGATTCTGATA+AGG | - | chr5.4:64959143-64959162 | MS.gene80684:CDS | 25.0% |
| AAGGCGAATACATAAAAACT+TGG | - | chr5.4:64958938-64958957 | MS.gene80684:CDS | 30.0% | |
| ! | TTCTAGATCTGTTACTGATT+CGG | - | chr5.4:64959212-64959231 | MS.gene80684:CDS | 30.0% |
| AGGATTGGATTAACTCGATT+GGG | - | chr5.4:64959172-64959191 | MS.gene80684:CDS | 35.0% | |
| TGATTCTGATAAGGAGAAAG+AGG | - | chr5.4:64959152-64959171 | MS.gene80684:CDS | 35.0% | |
| !!! | GTTGGTTCGTTTTGAAACAA+GGG | - | chr5.4:64958968-64958987 | MS.gene80684:CDS | 35.0% |
| CTGATAAGGAGAAAGAGGAT+TGG | - | chr5.4:64959157-64959176 | MS.gene80684:CDS | 40.0% | |
| GAGGATTGGATTAACTCGAT+TGG | - | chr5.4:64959171-64959190 | MS.gene80684:CDS | 40.0% | |
| GCTACTTGTCTCACTGTTAA+AGG | - | chr5.4:64959057-64959076 | MS.gene80684:CDS | 40.0% | |
| TAAAAACTTGGCGAAGACGT+TGG | - | chr5.4:64958950-64958969 | MS.gene80684:CDS | 40.0% | |
| TGAAACAAGGGAAACTCTTC+TGG | - | chr5.4:64958980-64958999 | MS.gene80684:CDS | 40.0% | |
| !!! | CGTTGGTTCGTTTTGAAACA+AGG | - | chr5.4:64958967-64958986 | MS.gene80684:CDS | 40.0% |
| ACAGTGAGACAAGTAGCAAC+AGG | + | chr5.4:64959054-64959073 | None:intergenic | 45.0% | |
| CAGTGAGACAAGTAGCAACA+GGG | + | chr5.4:64959053-64959072 | None:intergenic | 45.0% | |
| GTGGAAAGTTCAAACGCGTA+GGG | + | chr5.4:64959105-64959124 | None:intergenic | 45.0% | |
| TGTGGAAAGTTCAAACGCGT+AGG | + | chr5.4:64959106-64959125 | None:intergenic | 45.0% | |
| ! | ACCGGCTGGTTAACAAAACA+AGG | - | chr5.4:64958919-64958938 | MS.gene80684:CDS | 45.0% |
| !!! | GCCTTGTTTTGTTAACCAGC+CGG | + | chr5.4:64958923-64958942 | None:intergenic | 45.0% |
| ATACATCGTATCAGCGCGTG+TGG | + | chr5.4:64959124-64959143 | None:intergenic | 50.0% | |
| ! | AACCGGTTCAACCACAAACC+CGG | - | chr5.4:64958855-64958874 | MS.gene80684:CDS | 50.0% |
| !! | TTAACCAGCCGGTTCGTTCT+GGG | + | chr5.4:64958912-64958931 | None:intergenic | 50.0% |
| ACAAACCCGGTCGACTACTC+CGG | - | chr5.4:64958868-64958887 | MS.gene80684:CDS | 55.0% | |
| GACCGGGTTTGTGGTTGAAC+CGG | + | chr5.4:64958860-64958879 | None:intergenic | 55.0% | |
| GCAACAGGGATAACACCACG+CGG | + | chr5.4:64959039-64959058 | None:intergenic | 55.0% | |
| GTTCGACCAGAATTCGACGC+CGG | + | chr5.4:64958890-64958909 | None:intergenic | 55.0% | |
| TGGTCGAACCCAGAACGAAC+CGG | - | chr5.4:64958901-64958920 | MS.gene80684:CDS | 55.0% | |
| ! | ACTACTCCGGCGTCGAATTC+TGG | - | chr5.4:64958881-64958900 | MS.gene80684:CDS | 55.0% |
| !! | GTTAACCAGCCGGTTCGTTC+TGG | + | chr5.4:64958913-64958932 | None:intergenic | 55.0% |
| ACACGCGCTTCTATACCGCG+TGG | - | chr5.4:64959021-64959040 | MS.gene80684:CDS | 60.0% | |
| AGTCTGTGGCGCTACGCAAC+CGG | - | chr5.4:64958838-64958857 | MS.gene80684:CDS | 60.0% | |
| GGAGTAGTCGACCGGGTTTG+TGG | + | chr5.4:64958869-64958888 | None:intergenic | 60.0% | |
| CGAACCCAGAACGAACCGGC+TGG | - | chr5.4:64958905-64958924 | MS.gene80684:CDS | 65.0% | |
| TCGACGCCGGAGTAGTCGAC+CGG | + | chr5.4:64958877-64958896 | None:intergenic | 65.0% | |
| CGACGCCGGAGTAGTCGACC+GGG | + | chr5.4:64958876-64958895 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 64958829 | 64959263 | 64958829 | ID=MS.gene80684 |
| chr5.4 | mRNA | 64958829 | 64959263 | 64958829 | ID=MS.gene80684.t1;Parent=MS.gene80684 |
| chr5.4 | exon | 64958829 | 64959263 | 64958829 | ID=MS.gene80684.t1.exon1;Parent=MS.gene80684.t1 |
| chr5.4 | CDS | 64958829 | 64959263 | 64958829 | ID=cds.MS.gene80684.t1;Parent=MS.gene80684.t1 |
| Gene Sequence |
| Protein sequence |
