Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80813.t1 | XP_003616621.1 | 99.3 | 144 | 1 | 0 | 1 | 144 | 1 | 144 | 8.80E-79 | 302.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80813.t1 | Q9ST43 | 70.2 | 141 | 40 | 1 | 4 | 142 | 3 | 143 | 1.2e-55 | 217.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80813.t1 | G7KGZ7 | 99.3 | 144 | 1 | 0 | 1 | 144 | 1 | 144 | 6.3e-79 | 302.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene80813 | MS.gene02572 | PPI |
| MS.gene80813 | MS.gene46264 | PPI |
| MS.gene013786 | MS.gene80813 | PPI |
| MS.gene80813 | MS.gene03201 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80813.t1 | MTR_5g082470 | 99.306 | 144 | 1 | 0 | 1 | 144 | 1 | 144 | 1.12e-105 | 297 |
| MS.gene80813.t1 | MTR_3g463520 | 72.340 | 141 | 37 | 1 | 4 | 142 | 3 | 143 | 6.84e-75 | 220 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80813.t1 | AT5G05710 | 80.142 | 141 | 27 | 1 | 3 | 142 | 2 | 142 | 9.42e-81 | 234 |
| MS.gene80813.t1 | AT2G29700 | 70.213 | 141 | 40 | 1 | 4 | 142 | 3 | 143 | 1.31e-71 | 211 |
Find 30 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTAACCAGCCGGTTCGTTC+TGG | 0.208272 | 5.3:+66728048 | None:intergenic |
| ACTACTCCGGCGTCGAATTC+TGG | 0.239180 | 5.3:-66728077 | MS.gene80813:CDS |
| GCTACTTGTCTCACTGTTAA+AGG | 0.239265 | 5.3:-66727901 | MS.gene80813:CDS |
| TGAAACAAGGGAAACTATTC+TGG | 0.254323 | 5.3:-66727978 | MS.gene80813:CDS |
| CCGGAATGGCAGCAAGTCTT+TGG | 0.341637 | 5.3:-66728134 | None:intergenic |
| TTAACCAGCCGGTTCGTTCT+GGG | 0.377723 | 5.3:+66728049 | None:intergenic |
| GAAATTGTTGATTATGATAA+CGG | 0.378590 | 5.3:-66727724 | MS.gene80813:CDS |
| TTCTAGATCTGTTACTGATT+CGG | 0.390424 | 5.3:-66727746 | MS.gene80813:CDS |
| GACCGGGTTTGTGGTTGAAC+CGG | 0.413116 | 5.3:+66728101 | None:intergenic |
| CGGATAAGGAGAAGGAGGAT+TGG | 0.422909 | 5.3:-66727801 | MS.gene80813:CDS |
| ACAGTGAGACAAGTAGCAAC+AGG | 0.427222 | 5.3:+66727907 | None:intergenic |
| AGTCTTTGGCGCTACGCAAC+CGG | 0.436791 | 5.3:-66728120 | MS.gene80813:CDS |
| TGGTCGAACCCAGAACGAAC+CGG | 0.518095 | 5.3:-66728057 | MS.gene80813:CDS |
| GAGGATTGGATTAACTCGAT+TGG | 0.520417 | 5.3:-66727787 | MS.gene80813:CDS |
| GGAGTAGTCGACCGGGTTTG+TGG | 0.532363 | 5.3:+66728092 | None:intergenic |
| ACAAACCCGGTCGACTACTC+CGG | 0.537859 | 5.3:-66728090 | MS.gene80813:CDS |
| TGCTGATTCGGATAAGGAGA+AGG | 0.557877 | 5.3:-66727809 | MS.gene80813:CDS |
| TCGACGCCGGAGTAGTCGAC+CGG | 0.566459 | 5.3:+66728084 | None:intergenic |
| AACCGGTTCAACCACAAACC+CGG | 0.568891 | 5.3:-66728103 | MS.gene80813:CDS |
| GTGGAAAGTTCAAACGCGTA+GGG | 0.587874 | 5.3:+66727856 | None:intergenic |
| ATACATCGTATCAGCGCGTG+TGG | 0.592591 | 5.3:+66727837 | None:intergenic |
| TGTGGAAAGTTCAAACGCGT+AGG | 0.611943 | 5.3:+66727855 | None:intergenic |
| CGAACCCAGAACGAACCGGC+TGG | 0.620445 | 5.3:-66728053 | MS.gene80813:CDS |
| CGACGCCGGAGTAGTCGACC+GGG | 0.622012 | 5.3:+66728085 | None:intergenic |
| ACCGGCTGGTTAACAAAACA+AGG | 0.628016 | 5.3:-66728039 | MS.gene80813:CDS |
| TGATTCGGATAAGGAGAAGG+AGG | 0.658919 | 5.3:-66727806 | MS.gene80813:CDS |
| CAGTGAGACAAGTAGCAACA+GGG | 0.674850 | 5.3:+66727908 | None:intergenic |
| GTTCGACCAGAATTCGACGC+CGG | 0.725090 | 5.3:+66728071 | None:intergenic |
| ACACGCGCTTCTATACCGCG+TGG | 0.787665 | 5.3:-66727937 | MS.gene80813:CDS |
| GCAACAGGGATAACACCACG+CGG | 0.840792 | 5.3:+66727922 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTGTTGATTATGATAA+CGG | - | chr5.3:66728122-66728141 | MS.gene80813:CDS | 20.0% |
| !!! | GATGTATTTTATTGCTGATT+CGG | - | chr5.3:66728025-66728044 | MS.gene80813:CDS | 25.0% |
| AAGGCGAATACATAAAAACA+TGG | - | chr5.3:66727826-66727845 | MS.gene80813:CDS | 30.0% | |
| ! | TTCTAGATCTGTTACTGATT+CGG | - | chr5.3:66728100-66728119 | MS.gene80813:CDS | 30.0% |
| !!! | TTTTATTGCTGATTCGGATA+AGG | - | chr5.3:66728031-66728050 | MS.gene80813:CDS | 30.0% |
| TGAAACAAGGGAAACTATTC+TGG | - | chr5.3:66727868-66727887 | MS.gene80813:CDS | 35.0% | |
| !!! | GTTGGTTCGTTTTGAAACAA+GGG | - | chr5.3:66727856-66727875 | MS.gene80813:CDS | 35.0% |
| GAGGATTGGATTAACTCGAT+TGG | - | chr5.3:66728059-66728078 | MS.gene80813:CDS | 40.0% | |
| GCTACTTGTCTCACTGTTAA+AGG | - | chr5.3:66727945-66727964 | MS.gene80813:CDS | 40.0% | |
| TAAAAACATGGCGAAGACGT+TGG | - | chr5.3:66727838-66727857 | MS.gene80813:CDS | 40.0% | |
| !!! | CGTTGGTTCGTTTTGAAACA+AGG | - | chr5.3:66727855-66727874 | MS.gene80813:CDS | 40.0% |
| ACAGTGAGACAAGTAGCAAC+AGG | + | chr5.3:66727942-66727961 | None:intergenic | 45.0% | |
| CAGTGAGACAAGTAGCAACA+GGG | + | chr5.3:66727941-66727960 | None:intergenic | 45.0% | |
| GTGGAAAGTTCAAACGCGTA+GGG | + | chr5.3:66727993-66728012 | None:intergenic | 45.0% | |
| TGTGGAAAGTTCAAACGCGT+AGG | + | chr5.3:66727994-66728013 | None:intergenic | 45.0% | |
| ! | ACCGGCTGGTTAACAAAACA+AGG | - | chr5.3:66727807-66727826 | MS.gene80813:CDS | 45.0% |
| ! | TGATTCGGATAAGGAGAAGG+AGG | - | chr5.3:66728040-66728059 | MS.gene80813:CDS | 45.0% |
| !! | TGCTGATTCGGATAAGGAGA+AGG | - | chr5.3:66728037-66728056 | MS.gene80813:CDS | 45.0% |
| !!! | GCCTTGTTTTGTTAACCAGC+CGG | + | chr5.3:66727811-66727830 | None:intergenic | 45.0% |
| ATACATCGTATCAGCGCGTG+TGG | + | chr5.3:66728012-66728031 | None:intergenic | 50.0% | |
| CGGATAAGGAGAAGGAGGAT+TGG | - | chr5.3:66728045-66728064 | MS.gene80813:CDS | 50.0% | |
| ! | AACCGGTTCAACCACAAACC+CGG | - | chr5.3:66727743-66727762 | MS.gene80813:CDS | 50.0% |
| !! | TTAACCAGCCGGTTCGTTCT+GGG | + | chr5.3:66727800-66727819 | None:intergenic | 50.0% |
| ACAAACCCGGTCGACTACTC+CGG | - | chr5.3:66727756-66727775 | MS.gene80813:CDS | 55.0% | |
| AGTCTTTGGCGCTACGCAAC+CGG | - | chr5.3:66727726-66727745 | MS.gene80813:CDS | 55.0% | |
| GACCGGGTTTGTGGTTGAAC+CGG | + | chr5.3:66727748-66727767 | None:intergenic | 55.0% | |
| GCAACAGGGATAACACCACG+CGG | + | chr5.3:66727927-66727946 | None:intergenic | 55.0% | |
| GTTCGACCAGAATTCGACGC+CGG | + | chr5.3:66727778-66727797 | None:intergenic | 55.0% | |
| TGGTCGAACCCAGAACGAAC+CGG | - | chr5.3:66727789-66727808 | MS.gene80813:CDS | 55.0% | |
| ! | ACTACTCCGGCGTCGAATTC+TGG | - | chr5.3:66727769-66727788 | MS.gene80813:CDS | 55.0% |
| !! | GTTAACCAGCCGGTTCGTTC+TGG | + | chr5.3:66727801-66727820 | None:intergenic | 55.0% |
| ACACGCGCTTCTATACCGCG+TGG | - | chr5.3:66727909-66727928 | MS.gene80813:CDS | 60.0% | |
| GGAGTAGTCGACCGGGTTTG+TGG | + | chr5.3:66727757-66727776 | None:intergenic | 60.0% | |
| CGAACCCAGAACGAACCGGC+TGG | - | chr5.3:66727793-66727812 | MS.gene80813:CDS | 65.0% | |
| TCGACGCCGGAGTAGTCGAC+CGG | + | chr5.3:66727765-66727784 | None:intergenic | 65.0% | |
| CGACGCCGGAGTAGTCGACC+GGG | + | chr5.3:66727764-66727783 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 66727717 | 66728151 | 66727717 | ID=MS.gene80813 |
| chr5.3 | mRNA | 66727717 | 66728151 | 66727717 | ID=MS.gene80813.t1;Parent=MS.gene80813 |
| chr5.3 | exon | 66727717 | 66728151 | 66727717 | ID=MS.gene80813.t1.exon1;Parent=MS.gene80813.t1 |
| chr5.3 | CDS | 66727717 | 66728151 | 66727717 | ID=cds.MS.gene80813.t1;Parent=MS.gene80813.t1 |
| Gene Sequence |
| Protein sequence |
