Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81046.t1 | XP_003627466.2 | 95.1 | 164 | 8 | 0 | 1 | 164 | 1 | 164 | 8.70E-83 | 316.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81046.t1 | G7L9W5 | 95.1 | 164 | 8 | 0 | 1 | 164 | 1 | 164 | 6.3e-83 | 316.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene81046 | 0.811161 | 8.04E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene81046 | 0.828629 | 8.09E-55 | -1.69E-46 |
| MS.gene051720 | MS.gene81046 | 0.816495 | 5.37E-52 | -1.69E-46 |
| MS.gene05458 | MS.gene81046 | 0.80334 | 3.65E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene81046 | 0.809038 | 2.31E-50 | -1.69E-46 |
| MS.gene056308 | MS.gene81046 | 0.800408 | 1.46E-48 | -1.69E-46 |
| MS.gene060519 | MS.gene81046 | 0.825045 | 5.80E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81046.t1 | MTR_8g023390 | 95.122 | 164 | 8 | 0 | 1 | 164 | 1 | 164 | 2.29e-115 | 323 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAACAAACTTGTTATTAA+AGG | 0.220128 | 8.4:+71512524 | None:intergenic |
| GTTATTAAAGGTCCAGAAAA+TGG | 0.227908 | 8.4:+71512536 | None:intergenic |
| TTCTCTTCTGATCTCTTTGT+TGG | 0.245231 | 8.4:+71512500 | None:intergenic |
| CTTCCCATCATAACCTTTGT+AGG | 0.252175 | 8.4:+71512568 | None:intergenic |
| AAAAGACAAGGTCATGTTTC+TGG | 0.282772 | 8.4:-71512605 | MS.gene81046:CDS |
| TTTCCCTTTCTCACTTTCTC+TGG | 0.320462 | 8.4:+71512728 | None:intergenic |
| TGTTGATGGAGAGTCATATA+AGG | 0.357605 | 8.4:+71512470 | None:intergenic |
| CCAAGGCATTATGATGACTA+TGG | 0.364903 | 8.4:-71513362 | MS.gene81046:CDS |
| GGAAAAGTTCCCTGGTTTCA+TGG | 0.375096 | 8.4:-71512697 | MS.gene81046:CDS |
| TTTAGCTATAGAATTCTTGT+TGG | 0.388291 | 8.4:+71512637 | None:intergenic |
| CCTCATCCTGTTCAAAACTA+TGG | 0.408153 | 8.4:-71512440 | MS.gene81046:CDS |
| AACTCCTACAAAGGTTATGA+TGG | 0.409233 | 8.4:-71512572 | MS.gene81046:CDS |
| CCTTTGTAGGAGTTAAGTAT+TGG | 0.416425 | 8.4:+71512581 | None:intergenic |
| AGGACCAGAGAAAGTGAGAA+AGG | 0.425794 | 8.4:-71512732 | MS.gene81046:CDS |
| CTATGGATTTGATCTGCAAC+AGG | 0.457681 | 8.4:-71513345 | MS.gene81046:CDS |
| AAACTATGGTCCTCTTTATG+TGG | 0.476561 | 8.4:-71512426 | MS.gene81046:CDS |
| TGAGAAAGGGAAAGAAATCA+TGG | 0.486173 | 8.4:-71512718 | MS.gene81046:CDS |
| GGACCAGAGAAAGTGAGAAA+GGG | 0.486612 | 8.4:-71512731 | MS.gene81046:CDS |
| CAAGTTTCCACCATGAAACC+AGG | 0.489863 | 8.4:+71512687 | None:intergenic |
| CTAGTTTCTGGCTGAAGCAA+AGG | 0.513346 | 8.4:-71512819 | MS.gene81046:intron |
| AGTTAAGTAACCACATAAAG+AGG | 0.516609 | 8.4:+71512416 | None:intergenic |
| GAAATCATGGAAAAGTTCCC+TGG | 0.522925 | 8.4:-71512705 | MS.gene81046:CDS |
| CCATAGTCATCATAATGCCT+TGG | 0.544319 | 8.4:+71513362 | None:intergenic |
| CCAATACTTAACTCCTACAA+AGG | 0.560845 | 8.4:-71512581 | MS.gene81046:CDS |
| TCAACAGTGTACCCTGAAGA+AGG | 0.568735 | 8.4:-71512767 | MS.gene81046:CDS |
| ACTCCTACAAAGGTTATGAT+GGG | 0.578643 | 8.4:-71512571 | MS.gene81046:CDS |
| CTGGCTGAAGCAAAGGAGCA+TGG | 0.602825 | 8.4:-71512812 | MS.gene81046:CDS |
| GAAACTTGACAAGAAAAGCA+AGG | 0.632342 | 8.4:-71512672 | MS.gene81046:CDS |
| AAAGTTCCCTGGTTTCATGG+TGG | 0.673568 | 8.4:-71512694 | MS.gene81046:CDS |
| ACAATTCTTCAACACAACCA+AGG | 0.687130 | 8.4:-71513379 | MS.gene81046:CDS |
| AAAGTTTCTGAGAAAAGACA+AGG | 0.688919 | 8.4:-71512617 | MS.gene81046:CDS |
| AAGTTTCCACCATGAAACCA+GGG | 0.691429 | 8.4:+71512688 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTGATAATATTATCTAA+TGG | - | chr8.4:71512875-71512894 | MS.gene81046:intron | 10.0% |
| !!! | TTAAACAAACTTGTTATTAA+AGG | + | chr8.4:71513283-71513302 | None:intergenic | 15.0% |
| !!! | TTTAGAGTTTTACAAAATCA+CGG | - | chr8.4:71512567-71512586 | MS.gene81046:CDS | 20.0% |
| ! | TAAAACCAAAAATTGTACCA+GGG | + | chr8.4:71512491-71512510 | None:intergenic | 25.0% |
| ! | TTAAAACCAAAAATTGTACC+AGG | + | chr8.4:71512492-71512511 | None:intergenic | 25.0% |
| ! | TTAAAACTTCAACAAAACCA+CGG | + | chr8.4:71512768-71512787 | None:intergenic | 25.0% |
| ! | TTTAGCTATAGAATTCTTGT+TGG | + | chr8.4:71513170-71513189 | None:intergenic | 25.0% |
| !! | TTTTGTGCAAGTTTGAATAA+CGG | - | chr8.4:71512717-71512736 | MS.gene81046:CDS | 25.0% |
| !!! | AGATTTTCACTCTTTGATTT+TGG | - | chr8.4:71512956-71512975 | MS.gene81046:intron | 25.0% |
| AAACATAGTTACTGCATGTT+TGG | - | chr8.4:71512510-71512529 | MS.gene81046:CDS | 30.0% | |
| ATGGTGAATTTGACATAATC+AGG | + | chr8.4:71512824-71512843 | None:intergenic | 30.0% | |
| GAAGAAGGATCTAAGAAAAA+AGG | - | chr8.4:71513052-71513071 | MS.gene81046:intron | 30.0% | |
| GTTATTAAAGGTCCAGAAAA+TGG | + | chr8.4:71513271-71513290 | None:intergenic | 30.0% | |
| TGGTGAATTTGACATAATCA+GGG | + | chr8.4:71512823-71512842 | None:intergenic | 30.0% | |
| ! | AAAGTTTCTGAGAAAAGACA+AGG | - | chr8.4:71513187-71513206 | MS.gene81046:intron | 30.0% |
| ! | AAGCAAAAGAATCCATTTTC+TGG | - | chr8.4:71513256-71513275 | MS.gene81046:intron | 30.0% |
| ! | ACTAACTGCATTTGAAATCA+TGG | + | chr8.4:71512617-71512636 | None:intergenic | 30.0% |
| ! | TTTTCTTAGATCCTTCTTCA+GGG | + | chr8.4:71513051-71513070 | None:intergenic | 30.0% |
| ! | TTTTTCTTAGATCCTTCTTC+AGG | + | chr8.4:71513052-71513071 | None:intergenic | 30.0% |
| AAAAGACAAGGTCATGTTTC+TGG | - | chr8.4:71513199-71513218 | MS.gene81046:intron | 35.0% | |
| AAACTATGGTCCTCTTTATG+TGG | - | chr8.4:71513378-71513397 | MS.gene81046:CDS | 35.0% | |
| AACTCCTACAAAGGTTATGA+TGG | - | chr8.4:71513232-71513251 | MS.gene81046:intron | 35.0% | |
| ACAATTCTTCAACACAACCA+AGG | - | chr8.4:71512425-71512444 | MS.gene81046:CDS | 35.0% | |
| ACTCCTACAAAGGTTATGAT+GGG | - | chr8.4:71513233-71513252 | MS.gene81046:intron | 35.0% | |
| AGTTTGAATAACGGTGAGTT+TGG | - | chr8.4:71512726-71512745 | MS.gene81046:CDS | 35.0% | |
| CCAATACTTAACTCCTACAA+AGG | - | chr8.4:71513223-71513242 | MS.gene81046:intron | 35.0% | |
| CCTTTGTAGGAGTTAAGTAT+TGG | + | chr8.4:71513226-71513245 | None:intergenic | 35.0% | |
| GAAACTTGACAAGAAAAGCA+AGG | - | chr8.4:71513132-71513151 | MS.gene81046:intron | 35.0% | |
| TGAGAAAGGGAAAGAAATCA+TGG | - | chr8.4:71513086-71513105 | MS.gene81046:intron | 35.0% | |
| TTACTGCATGTTTGGATTCA+CGG | - | chr8.4:71512518-71512537 | MS.gene81046:CDS | 35.0% | |
| TTCGTGCATGTTTAGATTGA+TGG | + | chr8.4:71512843-71512862 | None:intergenic | 35.0% | |
| TTCTCTTCTGATCTCTTTGT+TGG | + | chr8.4:71513307-71513326 | None:intergenic | 35.0% | |
| ! | CTTTTCCCTGGTACAATTTT+TGG | - | chr8.4:71512483-71512502 | MS.gene81046:CDS | 35.0% |
| ! | GATGAGGACTATTTTGTTGA+TGG | + | chr8.4:71513351-71513370 | None:intergenic | 35.0% |
| ! | TGTTGATGGAGAGTCATATA+AGG | + | chr8.4:71513337-71513356 | None:intergenic | 35.0% |
| AAGTTTCCACCATGAAACCA+GGG | + | chr8.4:71513119-71513138 | None:intergenic | 40.0% | |
| CCATAGTCATCATAATGCCT+TGG | + | chr8.4:71512445-71512464 | None:intergenic | 40.0% | |
| CCTCATCCTGTTCAAAACTA+TGG | - | chr8.4:71513364-71513383 | MS.gene81046:CDS | 40.0% | |
| CTATGGATTTGATCTGCAAC+AGG | - | chr8.4:71512459-71512478 | MS.gene81046:CDS | 40.0% | |
| CTTCCCATCATAACCTTTGT+AGG | + | chr8.4:71513239-71513258 | None:intergenic | 40.0% | |
| GAAATCATGGAAAAGTTCCC+TGG | - | chr8.4:71513099-71513118 | MS.gene81046:intron | 40.0% | |
| GCAAAATCACAATAACACCG+TGG | - | chr8.4:71512748-71512767 | MS.gene81046:CDS | 40.0% | |
| TTTCCCTTTCTCACTTTCTC+TGG | + | chr8.4:71513079-71513098 | None:intergenic | 40.0% | |
| ! | CCAAGGCATTATGATGACTA+TGG | - | chr8.4:71512442-71512461 | MS.gene81046:CDS | 40.0% |
| !!! | AGAGGACCATAGTTTTGAAC+AGG | + | chr8.4:71513373-71513392 | None:intergenic | 40.0% |
| !!! | CCATAGTTTTGAACAGGATG+AGG | + | chr8.4:71513367-71513386 | None:intergenic | 40.0% |
| !!! | TTTTGGCTCTCACTAGTTTC+TGG | - | chr8.4:71512973-71512992 | MS.gene81046:intron | 40.0% |
| AAAGTTCCCTGGTTTCATGG+TGG | - | chr8.4:71513110-71513129 | MS.gene81046:intron | 45.0% | |
| AGGACCAGAGAAAGTGAGAA+AGG | - | chr8.4:71513072-71513091 | MS.gene81046:intron | 45.0% | |
| CAAGTTTCCACCATGAAACC+AGG | + | chr8.4:71513120-71513139 | None:intergenic | 45.0% | |
| GGAAAAGTTCCCTGGTTTCA+TGG | - | chr8.4:71513107-71513126 | MS.gene81046:intron | 45.0% | |
| GGACCAGAGAAAGTGAGAAA+GGG | - | chr8.4:71513073-71513092 | MS.gene81046:intron | 45.0% | |
| TCAACAGTGTACCCTGAAGA+AGG | - | chr8.4:71513037-71513056 | MS.gene81046:intron | 45.0% | |
| !! | CTAGTTTCTGGCTGAAGCAA+AGG | - | chr8.4:71512985-71513004 | MS.gene81046:intron | 45.0% |
| ! | TCTGCAACAGGACTTTTCCC+TGG | - | chr8.4:71512471-71512490 | MS.gene81046:CDS | 50.0% |
| CTGGCTGAAGCAAAGGAGCA+TGG | - | chr8.4:71512992-71513011 | MS.gene81046:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 71512418 | 71513408 | 71512418 | ID=MS.gene81046 |
| chr8.4 | mRNA | 71512418 | 71513408 | 71512418 | ID=MS.gene81046.t1;Parent=MS.gene81046 |
| chr8.4 | exon | 71513334 | 71513408 | 71513334 | ID=MS.gene81046.t1.exon1;Parent=MS.gene81046.t1 |
| chr8.4 | CDS | 71513334 | 71513408 | 71513334 | ID=cds.MS.gene81046.t1;Parent=MS.gene81046.t1 |
| chr8.4 | exon | 71512418 | 71512837 | 71512418 | ID=MS.gene81046.t1.exon2;Parent=MS.gene81046.t1 |
| chr8.4 | CDS | 71512418 | 71512837 | 71512418 | ID=cds.MS.gene81046.t1;Parent=MS.gene81046.t1 |
| Gene Sequence |
| Protein sequence |
