AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81960.t1 MTR_3g090120 97.837 416 9 0 1 416 51 466 0.0 845
MS.gene81960.t1 MTR_8g093530 53.448 406 171 2 9 414 34 421 1.70e-156 449
MS.gene81960.t1 MTR_7g094750 45.634 355 174 5 77 416 89 439 2.70e-108 327
MS.gene81960.t1 MTR_2g022560 41.962 367 193 6 59 411 79 439 1.48e-98 301
MS.gene81960.t1 MTR_7g109550 43.235 340 181 5 79 412 137 470 1.73e-98 302
MS.gene81960.t1 MTR_4g121560 42.029 345 185 4 78 410 113 454 1.60e-93 289
MS.gene81960.t1 MTR_1g058380 40.000 350 197 6 77 415 53 400 4.91e-91 281
MS.gene81960.t1 MTR_7g094750 51.852 243 113 1 77 319 89 327 5.61e-90 275
MS.gene81960.t1 MTR_5g059400 36.247 389 209 7 37 410 152 516 2.05e-87 275
MS.gene81960.t1 MTR_1g116130 36.247 389 209 7 37 410 152 516 2.05e-87 275
MS.gene81960.t1 MTR_5g033800 38.032 376 207 8 52 411 120 485 1.69e-85 269
MS.gene81960.t1 MTR_7g093450 39.503 362 197 6 66 413 238 591 1.73e-85 272
MS.gene81960.t1 MTR_0136s0010 37.845 399 217 7 39 412 120 512 1.51e-84 268
MS.gene81960.t1 MTR_8g069790 39.889 361 193 9 67 411 166 518 1.52e-83 265
MS.gene81960.t1 MTR_4g126950 35.967 367 212 7 62 416 98 453 2.01e-83 263
MS.gene81960.t1 MTR_2g073500 39.130 345 178 10 76 411 26 347 3.11e-76 241
MS.gene81960.t1 MTR_5g034810 36.769 359 198 10 61 411 17 354 2.19e-72 231
MS.gene81960.t1 MTR_3g464570 35.734 361 189 9 73 410 37 377 2.16e-71 229
MS.gene81960.t1 MTR_2g015720 38.551 345 186 9 71 409 34 358 7.11e-71 227
MS.gene81960.t1 MTR_1g111010 37.830 341 183 12 74 410 27 342 1.58e-70 226
MS.gene81960.t1 MTR_5g071350 33.862 378 203 9 62 410 41 400 1.46e-69 225
MS.gene81960.t1 MTR_4g088030 38.462 338 183 8 79 410 33 351 2.98e-69 223
MS.gene81960.t1 MTR_3g049330 34.637 358 209 7 60 410 12 351 3.55e-69 223
MS.gene81960.t1 MTR_4g016940 34.551 356 198 8 77 409 84 427 5.15e-69 224
MS.gene81960.t1 MTR_4g087730 37.870 338 185 7 79 410 45 363 6.93e-69 222
MS.gene81960.t1 MTR_3g035830 34.203 345 186 8 77 407 29 346 7.46e-69 222
MS.gene81960.t1 MTR_3g021060 35.207 338 192 8 77 407 44 361 9.11e-69 222
MS.gene81960.t1 MTR_2g073510 38.719 359 190 10 61 409 15 353 1.43e-68 221
MS.gene81960.t1 MTR_4g079700 37.318 343 190 8 72 409 41 363 1.95e-68 221
MS.gene81960.t1 MTR_3g021090 34.985 343 182 8 77 405 29 344 1.32e-67 220
MS.gene81960.t1 MTR_5g033800 40.149 269 151 4 52 319 120 379 3.74e-67 218
MS.gene81960.t1 MTR_4g014440 31.622 370 220 8 67 412 50 410 2.44e-64 212
MS.gene81960.t1 MTR_1g050448 35.072 345 196 7 75 409 49 375 7.15e-63 207
MS.gene81960.t1 MTR_1g112210 35.135 370 200 9 58 409 35 382 2.04e-62 206
MS.gene81960.t1 MTR_2g091175 36.202 337 191 7 79 410 50 367 8.21e-62 204
MS.gene81960.t1 MTR_8g463790 32.249 369 210 9 67 411 59 411 1.03e-61 205
MS.gene81960.t1 MTR_1g112210 34.783 368 191 8 58 409 35 369 1.66e-60 201
MS.gene81960.t1 MTR_7g068920 34.072 361 193 9 77 409 78 421 1.83e-60 202
MS.gene81960.t1 MTR_3g070900 31.492 362 211 8 74 410 101 450 2.76e-59 200
MS.gene81960.t1 MTR_7g070060 30.749 374 215 10 71 412 51 412 6.66e-59 197
MS.gene81960.t1 MTR_4g014430 31.476 359 208 9 74 409 103 446 1.93e-58 197
MS.gene81960.t1 MTR_5g082880 30.278 360 215 8 79 412 165 514 2.37e-56 194
MS.gene81960.t1 MTR_3g071650 30.595 353 214 8 79 410 89 431 1.70e-54 187
MS.gene81960.t1 MTR_5g083260 30.447 358 216 8 75 410 79 425 1.77e-54 186
MS.gene81960.t1 MTR_3g464570 35.417 288 147 9 146 410 2 273 3.96e-47 163
MS.gene81960.t1 MTR_6g061710 29.491 373 198 12 78 409 166 514 3.58e-43 158
MS.gene81960.t1 MTR_7g070060 33.852 257 149 6 71 318 51 295 2.03e-42 151
MS.gene81960.t1 MTR_5g035080 28.851 409 233 16 35 409 36 420 1.59e-39 147
MS.gene81960.t1 MTR_4g125060 30.423 355 210 13 78 409 131 471 2.04e-39 147
MS.gene81960.t1 MTR_4g125060 30.423 355 210 13 78 409 170 510 4.22e-39 147
MS.gene81960.t1 MTR_4g125060 30.423 355 210 13 78 409 199 539 5.68e-39 147
MS.gene81960.t1 MTR_5g035090 28.235 425 255 13 21 414 27 432 5.03e-38 142
MS.gene81960.t1 MTR_8g104970 28.800 375 210 14 71 409 112 465 5.47e-38 143
MS.gene81960.t1 MTR_5g035150 29.730 370 200 13 78 409 84 431 8.36e-38 142
MS.gene81960.t1 MTR_1g076690 29.301 372 212 14 78 415 222 576 1.19e-37 144
MS.gene81960.t1 MTR_3g109840 29.351 385 218 15 58 409 52 415 2.65e-37 140
MS.gene81960.t1 MTR_7g033230 28.800 375 196 13 78 409 263 609 6.19e-37 142
MS.gene81960.t1 MTR_8g093870 27.451 357 238 10 68 409 122 472 1.06e-36 140
MS.gene81960.t1 MTR_7g070060 27.541 305 185 8 135 412 15 310 1.11e-36 136
MS.gene81960.t1 MTR_7g033230 28.800 375 196 13 78 409 333 679 1.33e-36 142
MS.gene81960.t1 MTR_4g092830 27.596 366 211 14 79 410 48 393 5.36e-36 137
MS.gene81960.t1 MTR_2g022580 47.967 123 64 0 143 265 2 124 2.11e-35 127
MS.gene81960.t1 MTR_6g007777 26.580 459 272 16 7 416 22 464 3.81e-33 130
MS.gene81960.t1 MTR_6g016880 27.198 364 222 11 73 409 72 419 2.19e-31 124
MS.gene81960.t1 MTR_8g027900 27.733 375 224 14 67 410 198 556 4.74e-31 125
MS.gene81960.t1 MTR_5g035070 29.670 364 208 14 78 409 86 433 1.52e-30 122
MS.gene81960.t1 MTR_6g016875 27.441 379 224 12 65 411 50 409 6.87e-29 117
MS.gene81960.t1 MTR_6g016240 26.087 368 231 14 70 409 62 416 2.27e-28 115
MS.gene81960.t1 MTR_7g019120 25.410 366 226 12 73 409 73 420 2.20e-27 113
MS.gene81960.t1 MTR_8g099300 25.201 373 212 15 79 410 74 420 2.59e-27 112
MS.gene81960.t1 MTR_2g016060 26.503 366 229 12 74 412 50 402 2.18e-26 110
MS.gene81960.t1 MTR_5g035120 44.086 93 48 1 79 171 75 163 5.20e-22 92.8
MS.gene81960.t1 MTR_7g019140 38.384 99 57 1 73 171 73 167 3.01e-19 85.1
MS.gene81960.t1 MTR_5g069320 26.523 279 161 8 129 402 147 386 1.06e-17 84.7
MS.gene81960.t1 MTR_5g035140 26.772 254 140 10 200 416 28 272 5.06e-14 72.4
MS.gene81960.t1 MTR_3g021150 30.952 126 60 4 286 397 6 118 2.69e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81960.t1 AT1G48880 57.250 400 149 4 13 412 65 442 4.02e-170 484
MS.gene81960.t1 AT3G62390 41.243 354 193 5 68 412 127 474 4.20e-96 296
MS.gene81960.t1 AT3G06080 40.833 360 189 5 75 416 99 452 9.32e-96 295
MS.gene81960.t1 AT5G19160 37.264 424 232 7 20 416 35 451 1.17e-94 292
MS.gene81960.t1 AT5G06230 41.787 347 187 7 78 410 20 365 2.64e-89 275
MS.gene81960.t1 AT5G49340 41.143 350 183 8 78 410 99 442 7.91e-89 277
MS.gene81960.t1 AT5G06230 41.787 347 187 7 78 410 61 406 8.23e-89 275
MS.gene81960.t1 AT3G11570 42.462 325 172 5 99 410 98 420 3.00e-88 274
MS.gene81960.t1 AT1G60790 38.227 361 205 6 71 416 182 539 5.16e-84 267
MS.gene81960.t1 AT5G20590 37.101 345 201 6 78 410 140 480 2.37e-83 263
MS.gene81960.t1 AT5G06700 38.630 365 199 7 62 412 244 597 6.28e-83 266
MS.gene81960.t1 AT3G12060 37.433 374 214 7 54 412 170 538 7.85e-83 264
MS.gene81960.t1 AT2G34070 37.931 348 192 7 68 409 54 383 9.91e-79 248
MS.gene81960.t1 AT5G19160 39.355 310 179 2 20 324 35 340 1.70e-78 247
MS.gene81960.t1 AT3G06080 41.720 314 160 3 20 319 35 339 2.47e-78 246
MS.gene81960.t1 AT1G29050 38.462 338 183 7 78 409 60 378 1.07e-75 240
MS.gene81960.t1 AT3G14850 36.522 345 193 7 75 411 30 356 7.04e-73 232
MS.gene81960.t1 AT5G06230 44.033 243 134 1 79 319 62 304 1.25e-68 220
MS.gene81960.t1 AT3G62390 40.449 267 153 3 68 334 127 387 1.56e-68 223
MS.gene81960.t1 AT1G78710 36.522 345 181 9 79 410 38 357 2.09e-68 221
MS.gene81960.t1 AT2G42570 33.880 366 213 7 50 410 24 365 5.05e-67 218
MS.gene81960.t1 AT5G58600 33.894 357 196 11 76 410 63 401 2.00e-66 217
MS.gene81960.t1 AT2G30010 33.506 385 210 11 54 410 31 397 5.79e-65 213
MS.gene81960.t1 AT2G30010 33.506 385 210 11 54 410 74 440 1.48e-64 213
MS.gene81960.t1 AT5G01360 32.402 358 204 10 77 409 34 378 3.74e-64 211
MS.gene81960.t1 AT2G30900 36.416 346 192 11 76 411 40 367 5.58e-64 210
MS.gene81960.t1 AT2G31110 34.637 358 207 8 61 409 23 362 6.60e-64 209
MS.gene81960.t1 AT5G01360 32.402 358 204 10 77 409 90 434 1.33e-63 211
MS.gene81960.t1 AT1G73140 33.983 359 197 9 77 411 65 407 1.91e-63 209
MS.gene81960.t1 AT5G20590 38.430 242 145 2 78 319 140 377 1.17e-61 204
MS.gene81960.t1 AT3G54260 36.311 347 184 10 79 410 51 375 1.62e-61 204
MS.gene81960.t1 AT2G40160 29.508 427 254 13 21 413 14 427 3.95e-60 202
MS.gene81960.t1 AT2G40160 29.577 426 253 13 21 412 14 426 8.13e-60 201
MS.gene81960.t1 AT5G01620 32.102 352 206 8 79 409 110 449 9.09e-60 201
MS.gene81960.t1 AT5G01620 32.102 352 206 8 79 409 110 449 9.09e-60 201
MS.gene81960.t1 AT5G01620 32.102 352 206 8 79 409 118 457 1.05e-59 201
MS.gene81960.t1 AT2G38320 29.197 411 245 8 23 410 18 405 2.97e-59 199
MS.gene81960.t1 AT2G40150 29.891 368 213 10 77 412 69 423 1.35e-55 189
MS.gene81960.t1 AT2G40320 28.947 418 249 11 21 410 26 423 4.47e-54 186
MS.gene81960.t1 AT3G55990 28.230 418 239 10 44 412 81 486 4.80e-54 187
MS.gene81960.t1 AT3G11030 30.790 367 200 9 77 410 104 449 7.98e-54 186
MS.gene81960.t1 AT5G58600 36.179 246 137 6 75 318 62 289 1.89e-50 172
MS.gene81960.t1 AT2G38320 30.218 321 196 6 113 410 7 322 9.24e-50 171
MS.gene81960.t1 AT2G34070 36.122 263 148 6 153 409 1 249 2.49e-49 168
MS.gene81960.t1 AT1G78710 33.333 333 155 11 79 410 31 297 1.11e-48 167
MS.gene81960.t1 AT2G30900 39.331 239 125 7 76 312 40 260 1.94e-47 164
MS.gene81960.t1 AT3G14850 35.581 267 150 6 153 411 1 253 6.07e-46 159
MS.gene81960.t1 AT2G31110 36.567 268 144 7 61 324 23 268 1.67e-45 158
MS.gene81960.t1 AT2G40160 30.744 309 191 9 21 318 14 310 1.85e-43 154
MS.gene81960.t1 AT1G70230 33.151 365 178 17 79 409 79 411 6.47e-43 155
MS.gene81960.t1 AT5G64470 30.601 366 214 14 70 410 45 395 1.02e-40 149
MS.gene81960.t1 AT4G25360 27.952 415 240 16 43 416 134 530 1.07e-39 149
MS.gene81960.t1 AT4G25360 27.952 415 240 16 43 416 134 530 1.07e-39 149
MS.gene81960.t1 AT5G64470 29.839 372 215 14 70 410 45 401 1.28e-38 144
MS.gene81960.t1 AT5G51640 30.649 385 212 18 68 415 128 494 2.72e-37 142
MS.gene81960.t1 AT5G20680 29.609 358 221 16 72 409 111 457 7.65e-36 137
MS.gene81960.t1 AT1G01430 24.619 394 247 10 54 413 74 451 1.21e-35 137
MS.gene81960.t1 AT5G20680 29.609 358 221 16 72 409 187 533 1.97e-35 137
MS.gene81960.t1 AT5G20680 29.609 358 221 16 72 409 205 551 2.27e-35 137
MS.gene81960.t1 AT5G20680 29.609 358 221 16 72 409 205 551 2.27e-35 137
MS.gene81960.t1 AT5G20680 29.609 358 221 16 72 409 205 551 2.27e-35 137
MS.gene81960.t1 AT5G20680 29.609 358 221 16 72 409 205 551 2.27e-35 137
MS.gene81960.t1 AT4G01080 27.660 376 221 11 68 413 80 434 2.74e-35 135
MS.gene81960.t1 AT4G11090 27.273 440 251 18 12 410 15 426 4.96e-35 134
MS.gene81960.t1 AT5G64020 27.778 360 223 12 79 409 57 408 5.10e-35 134
MS.gene81960.t1 AT5G15890 29.379 354 209 14 79 409 187 522 6.87e-35 135
MS.gene81960.t1 AT2G37720 27.147 361 226 12 72 409 136 482 5.85e-34 132
MS.gene81960.t1 AT3G02440 28.283 396 224 14 53 413 110 480 8.77e-34 132
MS.gene81960.t1 AT3G02440 28.283 396 224 14 53 413 102 472 1.02e-33 132
MS.gene81960.t1 AT2G14530 29.282 362 208 14 79 409 62 406 2.13e-33 129
MS.gene81960.t1 AT2G31110 33.491 212 129 4 203 409 10 214 1.20e-30 117
MS.gene81960.t1 AT5G15900 27.559 381 230 15 64 414 50 414 4.54e-30 120
MS.gene81960.t1 AT5G15900 27.559 381 230 15 64 414 69 433 7.07e-30 120
MS.gene81960.t1 AT4G23790 28.647 377 219 15 68 410 68 428 9.92e-30 120
MS.gene81960.t1 AT3G28150 26.667 360 220 13 79 411 70 412 1.54e-26 110
MS.gene81960.t1 AT5G64470 30.576 278 166 11 70 333 45 309 4.98e-25 104
MS.gene81960.t1 AT5G64470 30.576 278 166 11 70 333 45 309 6.37e-25 104

Find 83 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAATGTGTTAGTCAAAAC+TGG 0.225698 3.2:-76362145 MS.gene81960:CDS
AAGAGTTATGATGAGATTAA+TGG 0.256943 3.2:-76362049 MS.gene81960:CDS
ATAAGATCACCAAACAAATT+AGG 0.268500 3.2:-76361622 MS.gene81960:CDS
CTAGGAATATCACAGTTCTT+AGG 0.278013 3.2:+76361794 None:intergenic
TAACTCTTAATCACTGAATT+AGG 0.281414 3.2:+76362064 None:intergenic
GATGTTCTTATATTCAATTC+AGG 0.293943 3.2:-76361437 MS.gene81960:CDS
ACCAAACAAATTAGGTTTCT+AGG 0.294028 3.2:-76361614 MS.gene81960:CDS
CCTTCATGTTAGTACTTTCA+TGG 0.298021 3.2:-76362111 MS.gene81960:CDS
ATGCTTGCAAAGTGACTAAA+AGG 0.302638 3.2:-76360399 MS.gene81960:CDS
AAGATAGAGGTTATACAAAA+TGG 0.305284 3.2:-76361826 MS.gene81960:CDS
AGAATTTCATTCCACATATC+AGG 0.307439 3.2:+76360181 None:intergenic
TAGTCACTTTGCAAGCATTA+TGG 0.311847 3.2:+76360404 None:intergenic
ACCTAGAAACCTAATTTGTT+TGG 0.335765 3.2:+76361613 None:intergenic
CTTTGCTCACCTGGGCATCT+TGG 0.341521 3.2:-76360661 MS.gene81960:CDS
GTTTGATGGAAGTTGGATTC+AGG 0.354028 3.2:-76361939 MS.gene81960:CDS
CATTGAGAAGCATCATATAA+AGG 0.356973 3.2:+76361893 None:intergenic
TCAAAACAGCTTTGCTCACC+TGG 0.358303 3.2:-76360670 MS.gene81960:CDS
TTAAAACCTCTTTCTGCAAA+TGG 0.366665 3.2:+76361869 None:intergenic
TTTGCTCACCTGGGCATCTT+GGG 0.367494 3.2:-76360660 MS.gene81960:CDS
CAATGTCCATTTGCAGAAAG+AGG 0.381573 3.2:-76361875 MS.gene81960:CDS
CTTCATGTTAGTACTTTCAT+GGG 0.409110 3.2:-76362110 MS.gene81960:CDS
GTAATGTGTTTGATGGAAGT+TGG 0.416256 3.2:-76361946 MS.gene81960:CDS
CTAAGAACTGTGATATTCCT+AGG 0.417542 3.2:-76361793 MS.gene81960:CDS
CATCTGAATCAACCCATTCA+TGG 0.430046 3.2:+76361456 None:intergenic
GGTGAAGACAACACTGAGAT+TGG 0.431850 3.2:-76361498 MS.gene81960:CDS
TGCAGTCATTGGTGTTTACC+TGG 0.434332 3.2:-76360208 MS.gene81960:CDS
TTATATTCAATTCAGGTCAC+TGG 0.436002 3.2:-76361430 MS.gene81960:CDS
TGCCTAGATTTGCGCCGAAA+AGG 0.436178 3.2:-76361520 MS.gene81960:CDS
CTGTACCTGATTGCAGTCAT+TGG 0.440386 3.2:-76360219 MS.gene81960:CDS
CTCTAAAATCCAATGTTTCA+AGG 0.441697 3.2:-76361976 MS.gene81960:CDS
AGGCCTTGGAAGAGAACTAT+AGG 0.446798 3.2:-76360379 MS.gene81960:CDS
GATGCATGTAATGTGTTTGA+TGG 0.447224 3.2:-76361953 MS.gene81960:CDS
ATTGAGAAGCATCATATAAA+GGG 0.448483 3.2:+76361894 None:intergenic
GCGAATGGGCGTAAAGATAG+AGG 0.453818 3.2:-76361839 MS.gene81960:CDS
TAGATGATATTAGCCATGAA+TGG 0.454788 3.2:-76361469 MS.gene81960:CDS
GCAAAGTGACTAAAAGGCCT+TGG 0.463270 3.2:-76360393 MS.gene81960:CDS
TGAAGGGTAATTGCAATGCT+TGG 0.470032 3.2:-76362180 MS.gene81960:CDS
CTTTCGAATCATCGCATTGG+AGG 0.470520 3.2:-76360592 MS.gene81960:intron
GCCTAGATTTGCGCCGAAAA+GGG 0.475483 3.2:-76361519 MS.gene81960:CDS
ACATGCATCCCTTGAAACAT+TGG 0.476011 3.2:+76361967 None:intergenic
ATTCATTGAGTAGGACTCAA+TGG 0.477060 3.2:-76361712 MS.gene81960:CDS
GTGATGGCCATGTGGGAACT+TGG 0.497057 3.2:-76360255 MS.gene81960:CDS
TGACGCCGATGGATGCATAT+AGG 0.500314 3.2:-76360279 MS.gene81960:CDS
CTTAATCACTGAATTAGGAG+TGG 0.505194 3.2:+76362069 None:intergenic
TTGCCTATAGTTCTCTTCCA+AGG 0.505458 3.2:+76360376 None:intergenic
AATGCGATGATTCGAAAGTT+CGG 0.509064 3.2:+76360597 None:intergenic
TACCTGGTGTTCCTGATATG+TGG 0.510262 3.2:-76360192 MS.gene81960:CDS
GCAAATCTAGGCACCGAACC+AGG 0.516119 3.2:+76361530 None:intergenic
AGATGATATTAGCCATGAAT+GGG 0.516712 3.2:-76361468 MS.gene81960:CDS
TTGTTGGTGATTCATTGAGT+AGG 0.521972 3.2:-76361721 MS.gene81960:CDS
AAACACCAATGACTGCAATC+AGG 0.522147 3.2:+76360214 None:intergenic
GAACTTTCGAATCATCGCAT+TGG 0.522383 3.2:-76360595 MS.gene81960:CDS
TTCATTGAGTAGGACTCAAT+GGG 0.525301 3.2:-76361711 MS.gene81960:CDS
GTTGGTAGCTCACTGAAGCT+TGG 0.529154 3.2:-76360713 MS.gene81960:CDS
TCTAAAATCCAATGTTTCAA+GGG 0.532501 3.2:-76361975 MS.gene81960:CDS
GAGGTTATACAAAATGGAGA+TGG 0.534592 3.2:-76361820 MS.gene81960:CDS
CAAAACAGCTTTGCTCACCT+GGG 0.540720 3.2:-76360669 MS.gene81960:CDS
TTTAGTCACAAAATTATGAA+GGG 0.543395 3.2:-76362196 None:intergenic
TATTCAATTCAGGTCACTGG+TGG 0.549775 3.2:-76361427 MS.gene81960:CDS
AAGAGTGTTTATGAAATCAA+AGG 0.567848 3.2:-76361647 MS.gene81960:CDS
GTCACGTGCAGCACCGTCAC+AGG 0.572514 3.2:+76360298 None:intergenic
TTAATCACTGAATTAGGAGT+GGG 0.574496 3.2:+76362070 None:intergenic
ATTCAGGTCACTGGTGGACA+AGG 0.579515 3.2:-76361421 MS.gene81960:CDS
ATTATCTACCCAAGATGCCC+AGG 0.580934 3.2:+76360652 None:intergenic
TTGATATGTATGTTAATGAC+AGG 0.583676 3.2:-76361683 MS.gene81960:CDS
ATGGATGCATATAGGAGTGA+TGG 0.585120 3.2:-76360271 MS.gene81960:CDS
CCATGAAAGTACTAACATGA+AGG 0.587525 3.2:+76362111 None:intergenic
AAATTAGGTTTCTAGGTGTG+AGG 0.589767 3.2:-76361607 MS.gene81960:CDS
GGTGCTGCACGTGACGCCGA+TGG 0.591102 3.2:-76360290 MS.gene81960:CDS
ACAAGTTCAGAGCTATGTTG+AGG 0.591680 3.2:-76361999 MS.gene81960:CDS
TGACTGCAATCAGGTACAGA+TGG 0.595507 3.2:+76360223 None:intergenic
AGAATACTCGAAAATCTTCG+TGG 0.596909 3.2:-76361758 MS.gene81960:CDS
GAATACTCGAAAATCTTCGT+GGG 0.608334 3.2:-76361757 MS.gene81960:CDS
TCAATGTGTTAGTCAAAACT+GGG 0.608452 3.2:-76362144 MS.gene81960:CDS
AAATCTTCGTGGGAAAAGAG+TGG 0.610197 3.2:-76361747 MS.gene81960:CDS
ATTCTACGTGCATCAAACCT+AGG 0.613739 3.2:+76361776 None:intergenic
TCACTCCTATATGCATCCAT+CGG 0.625934 3.2:+76360274 None:intergenic
TTCCACATATCAGGAACACC+AGG 0.639295 3.2:+76360190 None:intergenic
TGTCACTCCAAGTTCCCACA+TGG 0.643897 3.2:+76360248 None:intergenic
TTGGAAGAGAACTATAGGCA+AGG 0.649599 3.2:-76360374 MS.gene81960:CDS
TATAGGAGTGATGGCCATGT+GGG 0.667902 3.2:-76360262 MS.gene81960:CDS
AGATATGAGTGCTCCTGTGA+CGG 0.684121 3.2:-76360311 MS.gene81960:CDS
ATATAGGAGTGATGGCCATG+TGG 0.734312 3.2:-76360263 MS.gene81960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAACAAAATGAATAATA+TGG + chr3.2:76361773-76361792 None:intergenic 15.0%
!!! ATGATAGATATACATTTTTA+TGG - chr3.2:76361000-76361019 MS.gene81960:intron 15.0%
!!! ATTTTATCTAAAGAATTACA+GGG + chr3.2:76361293-76361312 None:intergenic 15.0%
!! GAAATTTATGAATTGTAAGA+AGG - chr3.2:76361171-76361190 MS.gene81960:intron 20.0%
!!! ATAAGATGTTTATCTTTTTG+TGG - chr3.2:76361886-76361905 MS.gene81960:CDS 20.0%
!!! ATCATGCATATGATAATTTT+GGG - chr3.2:76361263-76361282 MS.gene81960:intron 20.0%
!!! CATTTTATCTAAAGAATTAC+AGG + chr3.2:76361294-76361313 None:intergenic 20.0%
!!! GATTAATTTTGCTAATCTTT+TGG - chr3.2:76361827-76361846 MS.gene81960:CDS 20.0%
!!! TTAATTTTCATATTTGGATC+CGG - chr3.2:76361096-76361115 MS.gene81960:intron 20.0%
!!! TTGGACTTAATTTTCATATT+TGG - chr3.2:76361090-76361109 MS.gene81960:intron 20.0%
!!! TTTGTTTACATGCTTTTTTT+TGG - chr3.2:76361071-76361090 MS.gene81960:intron 20.0%
! AAGAGTTATGATGAGATTAA+TGG - chr3.2:76360276-76360295 MS.gene81960:CDS 25.0%
! AAGATAGAGGTTATACAAAA+TGG - chr3.2:76360499-76360518 MS.gene81960:intron 25.0%
! ATAAGATCACCAAACAAATT+AGG - chr3.2:76360703-76360722 MS.gene81960:CDS 25.0%
! ATTTATGAATTGTAAGAAGG+TGG - chr3.2:76361174-76361193 MS.gene81960:intron 25.0%
! CAAATAGTCTCATGAAAAAT+TGG - chr3.2:76361406-76361425 MS.gene81960:CDS 25.0%
! GATGTTCTTATATTCAATTC+AGG - chr3.2:76360888-76360907 MS.gene81960:intron 25.0%
! TAACTCTTAATCACTGAATT+AGG + chr3.2:76360264-76360283 None:intergenic 25.0%
! TTGATATGTATGTTAATGAC+AGG - chr3.2:76360642-76360661 MS.gene81960:CDS 25.0%
!! CATCATGCATATGATAATTT+TGG - chr3.2:76361262-76361281 MS.gene81960:intron 25.0%
!! CTTTTGATCATATCTGATAT+TGG + chr3.2:76361985-76362004 None:intergenic 25.0%
!! TAATTTTCATATTTGGATCC+GGG - chr3.2:76361097-76361116 MS.gene81960:intron 25.0%
!! TCTAAAATCCAATGTTTCAA+GGG - chr3.2:76360350-76360369 MS.gene81960:CDS 25.0%
!! TTTTAATAGAATTTCCATGC+AGG - chr3.2:76361505-76361524 MS.gene81960:CDS 25.0%
!!! AAGAGTGTTTATGAAATCAA+AGG - chr3.2:76360678-76360697 MS.gene81960:CDS 25.0%
!!! ATTGAGAAGCATCATATAAA+GGG + chr3.2:76360434-76360453 None:intergenic 25.0%
!!! GTTTTAATTGTTTTGCGAAT+GGG - chr3.2:76360472-76360491 MS.gene81960:intron 25.0%
!!! GTTTTGAAAGCTGAATTAAT+CGG + chr3.2:76361642-76361661 None:intergenic 25.0%
AATATTAATCCTAACCTGCA+TGG + chr3.2:76361522-76361541 None:intergenic 30.0%
ACCAAACAAATTAGGTTTCT+AGG - chr3.2:76360711-76360730 MS.gene81960:CDS 30.0%
AGAATTTCATTCCACATATC+AGG + chr3.2:76362147-76362166 None:intergenic 30.0%
AGATGATATTAGCCATGAAT+GGG - chr3.2:76360857-76360876 MS.gene81960:intron 30.0%
CTTCATGTTAGTACTTTCAT+GGG - chr3.2:76360215-76360234 MS.gene81960:CDS 30.0%
TAGATGATATTAGCCATGAA+TGG - chr3.2:76360856-76360875 MS.gene81960:intron 30.0%
TCAATGTGTTAGTCAAAACT+GGG - chr3.2:76360181-76360200 MS.gene81960:CDS 30.0%
TTAAAACCTCTTTCTGCAAA+TGG + chr3.2:76360459-76360478 None:intergenic 30.0%
TTAATCACTGAATTAGGAGT+GGG + chr3.2:76360258-76360277 None:intergenic 30.0%
TTATATTCAATTCAGGTCAC+TGG - chr3.2:76360895-76360914 MS.gene81960:intron 30.0%
TTCAATGTGTTAGTCAAAAC+TGG - chr3.2:76360180-76360199 MS.gene81960:CDS 30.0%
! ATGAAAAATTGGTCTCTTAG+TGG - chr3.2:76361417-76361436 MS.gene81960:CDS 30.0%
! TGTTTATCTTTTTGTGGTAG+TGG - chr3.2:76361892-76361911 MS.gene81960:CDS 30.0%
!! ACCTAGAAACCTAATTTGTT+TGG + chr3.2:76360715-76360734 None:intergenic 30.0%
!! CATTGAGAAGCATCATATAA+AGG + chr3.2:76360435-76360454 None:intergenic 30.0%
!! CTCTAAAATCCAATGTTTCA+AGG - chr3.2:76360349-76360368 MS.gene81960:CDS 30.0%
!!! GGTTTTAATTGTTTTGCGAA+TGG - chr3.2:76360471-76360490 MS.gene81960:intron 30.0%
AATGCGATGATTCGAAAGTT+CGG + chr3.2:76361731-76361750 None:intergenic 35.0%
AGAATACTCGAAAATCTTCG+TGG - chr3.2:76360567-76360586 MS.gene81960:intron 35.0%
ATAGAATTTCCATGCAGGTT+AGG - chr3.2:76361510-76361529 MS.gene81960:CDS 35.0%
ATGCTTGCAAAGTGACTAAA+AGG - chr3.2:76361926-76361945 MS.gene81960:CDS 35.0%
ATTCATTGAGTAGGACTCAA+TGG - chr3.2:76360613-76360632 MS.gene81960:CDS 35.0%
CCTTCATGTTAGTACTTTCA+TGG - chr3.2:76360214-76360233 MS.gene81960:CDS 35.0%
CTAACATGATTATCAGACAG+GGG - chr3.2:76361576-76361595 MS.gene81960:CDS 35.0%
CTAAGAACTGTGATATTCCT+AGG - chr3.2:76360532-76360551 MS.gene81960:intron 35.0%
CTAGGAATATCACAGTTCTT+AGG + chr3.2:76360534-76360553 None:intergenic 35.0%
CTTAATCACTGAATTAGGAG+TGG + chr3.2:76360259-76360278 None:intergenic 35.0%
GAATACTCGAAAATCTTCGT+GGG - chr3.2:76360568-76360587 MS.gene81960:intron 35.0%
GAGGTTATACAAAATGGAGA+TGG - chr3.2:76360505-76360524 MS.gene81960:intron 35.0%
GATGCATGTAATGTGTTTGA+TGG - chr3.2:76360372-76360391 MS.gene81960:CDS 35.0%
GCTAACATGATTATCAGACA+GGG - chr3.2:76361575-76361594 MS.gene81960:CDS 35.0%
GTAATGTGTTTGATGGAAGT+TGG - chr3.2:76360379-76360398 MS.gene81960:CDS 35.0%
TGCTAACATGATTATCAGAC+AGG - chr3.2:76361574-76361593 MS.gene81960:CDS 35.0%
TTCATTGAGTAGGACTCAAT+GGG - chr3.2:76360614-76360633 MS.gene81960:CDS 35.0%
! AAATTAGGTTTCTAGGTGTG+AGG - chr3.2:76360718-76360737 MS.gene81960:CDS 35.0%
! TAGTCACTTTGCAAGCATTA+TGG + chr3.2:76361924-76361943 None:intergenic 35.0%
!! CCATGAAAGTACTAACATGA+AGG + chr3.2:76360217-76360236 None:intergenic 35.0%
!! TTGTTGGTGATTCATTGAGT+AGG - chr3.2:76360604-76360623 MS.gene81960:CDS 35.0%
AAACACCAATGACTGCAATC+AGG + chr3.2:76362114-76362133 None:intergenic 40.0%
AAATCTTCGTGGGAAAAGAG+TGG - chr3.2:76360578-76360597 MS.gene81960:intron 40.0%
ACAAGTTCAGAGCTATGTTG+AGG - chr3.2:76360326-76360345 MS.gene81960:CDS 40.0%
ACAGTAAAGCATGCATTACC+CGG + chr3.2:76361118-76361137 None:intergenic 40.0%
ACATGCATCCCTTGAAACAT+TGG + chr3.2:76360361-76360380 None:intergenic 40.0%
ATGGATGCATATAGGAGTGA+TGG - chr3.2:76362054-76362073 MS.gene81960:CDS 40.0%
ATTCTACGTGCATCAAACCT+AGG + chr3.2:76360552-76360571 None:intergenic 40.0%
CAATGTCCATTTGCAGAAAG+AGG - chr3.2:76360450-76360469 MS.gene81960:intron 40.0%
CATCTGAATCAACCCATTCA+TGG + chr3.2:76360872-76360891 None:intergenic 40.0%
GAACTTTCGAATCATCGCAT+TGG - chr3.2:76361730-76361749 MS.gene81960:CDS 40.0%
GCTTCACGAGAAAAACAGAA+CGG + chr3.2:76360776-76360795 None:intergenic 40.0%
GTTTGATGGAAGTTGGATTC+AGG - chr3.2:76360386-76360405 MS.gene81960:CDS 40.0%
TATTCAATTCAGGTCACTGG+TGG - chr3.2:76360898-76360917 MS.gene81960:intron 40.0%
TCACTCCTATATGCATCCAT+CGG + chr3.2:76362054-76362073 None:intergenic 40.0%
TGAAGGGTAATTGCAATGCT+TGG - chr3.2:76360145-76360164 MS.gene81960:CDS 40.0%
TTGCCTATAGTTCTCTTCCA+AGG + chr3.2:76361952-76361971 None:intergenic 40.0%
TTGGAAGAGAACTATAGGCA+AGG - chr3.2:76361951-76361970 MS.gene81960:CDS 40.0%
! AGTGTTGTCTTCACCCTTTT+CGG + chr3.2:76360822-76360841 None:intergenic 40.0%
! GGGAAAAGAGTGGTTTTTGT+TGG - chr3.2:76360588-76360607 MS.gene81960:intron 40.0%
AGATATGAGTGCTCCTGTGA+CGG - chr3.2:76362014-76362033 MS.gene81960:CDS 45.0%
AGGCCTTGGAAGAGAACTAT+AGG - chr3.2:76361946-76361965 MS.gene81960:CDS 45.0%
ATATAGGAGTGATGGCCATG+TGG - chr3.2:76362062-76362081 MS.gene81960:CDS 45.0%
ATTATCTACCCAAGATGCCC+AGG + chr3.2:76361676-76361695 None:intergenic 45.0%
CAAAACAGCTTTGCTCACCT+GGG - chr3.2:76361656-76361675 MS.gene81960:CDS 45.0%
CTGTACCTGATTGCAGTCAT+TGG - chr3.2:76362106-76362125 MS.gene81960:CDS 45.0%
CTTTCGAATCATCGCATTGG+AGG - chr3.2:76361733-76361752 MS.gene81960:CDS 45.0%
GCAAAGTGACTAAAAGGCCT+TGG - chr3.2:76361932-76361951 MS.gene81960:CDS 45.0%
GGTGAAGACAACACTGAGAT+TGG - chr3.2:76360827-76360846 MS.gene81960:intron 45.0%
TACCTGGTGTTCCTGATATG+TGG - chr3.2:76362133-76362152 MS.gene81960:CDS 45.0%
TATAGGAGTGATGGCCATGT+GGG - chr3.2:76362063-76362082 MS.gene81960:CDS 45.0%
TCAAAACAGCTTTGCTCACC+TGG - chr3.2:76361655-76361674 MS.gene81960:CDS 45.0%
TGACTGCAATCAGGTACAGA+TGG + chr3.2:76362105-76362124 None:intergenic 45.0%
TTCCACATATCAGGAACACC+AGG + chr3.2:76362138-76362157 None:intergenic 45.0%
! TGCAGTCATTGGTGTTTACC+TGG - chr3.2:76362117-76362136 MS.gene81960:CDS 45.0%
! TTTTCTCGTGAAGCCTGGTT+CGG - chr3.2:76360782-76360801 MS.gene81960:intron 45.0%
!! TCTGTTTTTCTCGTGAAGCC+TGG - chr3.2:76360777-76360796 MS.gene81960:intron 45.0%
CATGATTATCAGACAGGGGC+TGG - chr3.2:76361580-76361599 MS.gene81960:CDS 50.0%
GCCTAGATTTGCGCCGAAAA+GGG - chr3.2:76360806-76360825 MS.gene81960:intron 50.0%
GCGAATGGGCGTAAAGATAG+AGG - chr3.2:76360486-76360505 MS.gene81960:intron 50.0%
TCATCGCATTGGAGGTGAGT+TGG - chr3.2:76361741-76361760 MS.gene81960:CDS 50.0%
TGACGCCGATGGATGCATAT+AGG - chr3.2:76362046-76362065 MS.gene81960:CDS 50.0%
TGCCTAGATTTGCGCCGAAA+AGG - chr3.2:76360805-76360824 MS.gene81960:intron 50.0%
TGTCACTCCAAGTTCCCACA+TGG + chr3.2:76362080-76362099 None:intergenic 50.0%
TTTGCTCACCTGGGCATCTT+GGG - chr3.2:76361665-76361684 MS.gene81960:CDS 50.0%
! ACCCTTTTCGGCGCAAATCT+AGG + chr3.2:76360810-76360829 None:intergenic 50.0%
! ATTCAGGTCACTGGTGGACA+AGG - chr3.2:76360904-76360923 MS.gene81960:intron 50.0%
!! AGACAGGGGCTGGTATTTTC+AGG - chr3.2:76361590-76361609 MS.gene81960:CDS 50.0%
!! GTTGGTAGCTCACTGAAGCT+TGG - chr3.2:76361612-76361631 MS.gene81960:CDS 50.0%
!!! AGGGGCTGGTATTTTCAGGT+TGG - chr3.2:76361594-76361613 MS.gene81960:CDS 50.0%
CTTTGCTCACCTGGGCATCT+TGG - chr3.2:76361664-76361683 MS.gene81960:CDS 55.0%
GTGATGGCCATGTGGGAACT+TGG - chr3.2:76362070-76362089 MS.gene81960:CDS 55.0%
!! GCAAATCTAGGCACCGAACC+AGG + chr3.2:76360798-76360817 None:intergenic 55.0%
GTCACGTGCAGCACCGTCAC+AGG + chr3.2:76362030-76362049 None:intergenic 65.0%
! GGTGCTGCACGTGACGCCGA+TGG - chr3.2:76362035-76362054 MS.gene81960:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 76360144 76362203 76360144 ID=MS.gene81960
chr3.2 mRNA 76360144 76362203 76360144 ID=MS.gene81960.t1;Parent=MS.gene81960
chr3.2 exon 76361404 76362203 76361404 ID=MS.gene81960.t1.exon1;Parent=MS.gene81960.t1
chr3.2 CDS 76361404 76362203 76361404 ID=cds.MS.gene81960.t1;Parent=MS.gene81960.t1
chr3.2 exon 76360593 76360751 76360593 ID=MS.gene81960.t1.exon2;Parent=MS.gene81960.t1
chr3.2 CDS 76360593 76360751 76360593 ID=cds.MS.gene81960.t1;Parent=MS.gene81960.t1
chr3.2 exon 76360144 76360435 76360144 ID=MS.gene81960.t1.exon3;Parent=MS.gene81960.t1
chr3.2 CDS 76360144 76360435 76360144 ID=cds.MS.gene81960.t1;Parent=MS.gene81960.t1
Gene Sequence

>MS.gene81960

ATGAAGGGTAATTGCAATGCTTGGATCTTAGTTTCTTTCAATGTGTTAGTCAAAACTGGGTCTTTGATTTCCTTCATGTTAGTACTTTCATGGGTTTATTCATATGCATTTCCCACTCCTAATTCAGTGATTAAGAGTTATGATGAGATTAATGGTAACATTAATAGTAATAGTTCTCATTTACAAGTTCAGAGCTATGTTGAGGCTCTAAAATCCAATGTTTCAAGGGATGCATGTAATGTGTTTGATGGAAGTTGGATTCAGGATGATAGTTATAGTTATAGTTACCCTTTATATGATGCTTCTCAATGTCCATTTGCAGAAAGAGGTTTTAATTGTTTTGCGAATGGGCGTAAAGATAGAGGTTATACAAAATGGAGATGGAAACCTAAGAACTGTGATATTCCTAGGTTTGATGCACGTAGAATACTCGAAAATCTTCGTGGGAAAAGAGTGGTTTTTGTTGGTGATTCATTGAGTAGGACTCAATGGGAGTCTTTGATATGTATGTTAATGACAGGTGTTGAGAATAAGAAGAGTGTTTATGAAATCAAAGGCAATAAGATCACCAAACAAATTAGGTTTCTAGGTGTGAGGTTTAGTTCTTTTGATGTTAGAATTGATTTTTACCGTTCTGTTTTTCTCGTGAAGCCTGGTTCGGTGCCTAGATTTGCGCCGAAAAGGGTGAAGACAACACTGAGATTGGACAAGATAGATGATATTAGCCATGAATGGGTTGATTCAGATGTTCTTATATTCAATTCAGGTCACTGGTGGACAAGGACTAAGCTTTTTGATATGTGAGTAACTTGATTCTACAAATTGAGATGTTTTTTTTTCTTCTTCTACTATGTAGATGATAGATATACATTTTTATGGTCTTATCTTGTTTCACAAATTAGAGATTTGATTTACTACTCTGCATATTTTGTTTACATGCTTTTTTTTGGACTTAATTTTCATATTTGGATCCGGGTAATGCATGCTTTACTGTCAGTTTGTTGATCAGAATCTTTATAAGTGATATGAAATTTATGAATTGTAAGAAGGTGGACATCTTATTGTGCTTTTGTGTTCAAAGAATCTTCAACTAGCGTTAGCTTTTCTGCAACTGAAAGCATCATGCATATGATAATTTTGGGTGATCCCTGTAATTCTTTAGATAAAATGATGTATAGCATTGAAACTGACTTGAACTTCAACAAGATGATGTTTTATCAGCATGACTATGATGAAACATGTAAAGTATACTTATTTTATTTCAAATAGTCTCATGAAAAATTGGTCTCTTAGTGGCTAGAATGCGTATTCTTTTGCATATTCATCTATCATAAATTTTTGTTTATGCGTGTGCACATGCATTTTAATAGAATTTCCATGCAGGTTAGGATTAATATTTTTATGAAAATTATCAACTATTTGCTGCTAATTGCTAACATGATTATCAGACAGGGGCTGGTATTTTCAGGTTGGTAGCTCACTGAAGCTTGGAATGCCGATTAATTCAGCTTTCAAAACAGCTTTGCTCACCTGGGCATCTTGGGTAGATAATACAGTTAACACAAACAGAACAAGAGTATTCTTCCGAACTTTCGAATCATCGCATTGGAGGTGAGTTGGTTGTAACCATATTATTCATTTTGTTTTGTCACTTTGCAGATGAGACAATGACAACAAAAAGATGATTAATTTTGCTAATCTTTTGGTAATACTAGAGCTATTGAACTTGATGATAAGACTTGATAAGATGTTTATCTTTTTGTGGTAGTGGACATAACCATAATGCTTGCAAAGTGACTAAAAGGCCTTGGAAGAGAACTATAGGCAAGGACAAAAGTCCAATATCAGATATGATCAAAAGAGTTGTGAAAGATATGAGTGCTCCTGTGACGGTGCTGCACGTGACGCCGATGGATGCATATAGGAGTGATGGCCATGTGGGAACTTGGAGTGACAAACCATCTGTACCTGATTGCAGTCATTGGTGTTTACCTGGTGTTCCTGATATGTGGAATGAAATTCTCTTCTCATACTTGCTACAAAAAGATGAAGCAAGTTAG
Protein sequence

>MS.gene81960.t1

MKGNCNAWILVSFNVLVKTGSLISFMLVLSWVYSYAFPTPNSVIKSYDEINGNINSNSSHLQVQSYVEALKSNVSRDACNVFDGSWIQDDSYSYSYPLYDASQCPFAERGFNCFANGRKDRGYTKWRWKPKNCDIPRFDARRILENLRGKRVVFVGDSLSRTQWESLICMLMTGVENKKSVYEIKGNKITKQIRFLGVRFSSFDVRIDFYRSVFLVKPGSVPRFAPKRVKTTLRLDKIDDISHEWVDSDVLIFNSGHWWTRTKLFDMGWYFQVGSSLKLGMPINSAFKTALLTWASWVDNTVNTNRTRVFFRTFESSHWSGHNHNACKVTKRPWKRTIGKDKSPISDMIKRVVKDMSAPVTVLHVTPMDAYRSDGHVGTWSDKPSVPDCSHWCLPGVPDMWNEILFSYLLQKDEAS