AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82016

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82016.t1 MTR_8g024520 93.993 283 15 2 1 282 1 282 0.0 509
MS.gene82016.t1 MTR_8g024520 91.519 283 20 2 1 282 1 280 0.0 509
MS.gene82016.t1 MTR_3g116130 56.000 200 72 9 18 209 23 214 6.24e-56 182
MS.gene82016.t1 MTR_3g116130 56.000 200 72 9 18 209 23 214 3.78e-55 182
MS.gene82016.t1 MTR_4g035310 45.725 269 111 10 9 247 1 264 1.81e-50 167
MS.gene82016.t1 MTR_4g035310 55.814 172 63 3 9 170 1 169 3.85e-50 166
MS.gene82016.t1 MTR_3g092490 52.000 175 75 6 1 170 1 171 1.94e-47 159
MS.gene82016.t1 MTR_3g092490 54.194 155 65 4 1 153 1 151 2.24e-47 156
MS.gene82016.t1 MTR_3g092490 52.273 176 74 6 1 170 1 172 7.16e-47 157
MS.gene82016.t1 MTR_5g064120 77.011 87 20 0 15 101 7 93 2.47e-45 154
MS.gene82016.t1 MTR_7g117495 63.636 99 34 1 9 105 1 99 2.10e-41 143
MS.gene82016.t1 MTR_7g117495 63.636 99 34 1 9 105 1 99 2.66e-41 143
MS.gene82016.t1 MTR_4g088010 48.810 84 43 0 20 103 72 155 6.75e-19 85.5
MS.gene82016.t1 MTR_8g463890 42.857 98 56 0 5 102 54 151 1.07e-18 85.9
MS.gene82016.t1 MTR_2g026180 36.066 122 74 2 25 143 5 125 4.44e-18 84.0
MS.gene82016.t1 MTR_3g055990 42.466 73 41 1 20 92 2 73 9.82e-17 80.1
MS.gene82016.t1 MTR_3g055990 42.466 73 41 1 20 92 2 73 9.97e-17 80.1
MS.gene82016.t1 MTR_7g088140 43.373 83 47 0 20 102 123 205 4.38e-16 78.2
MS.gene82016.t1 MTR_7g088140 43.373 83 47 0 20 102 123 205 7.16e-16 77.8
MS.gene82016.t1 MTR_5g088220 42.169 83 48 0 20 102 161 243 1.22e-15 77.0
MS.gene82016.t1 MTR_5g097580 44.000 75 41 1 25 99 7 80 2.01e-15 76.3
MS.gene82016.t1 MTR_5g097580 44.000 75 41 1 25 99 7 80 2.38e-15 76.3
MS.gene82016.t1 MTR_4g070190 43.421 76 42 1 25 99 9 84 1.69e-14 70.1
MS.gene82016.t1 MTR_4g070080 43.590 78 39 2 25 99 7 82 3.82e-14 68.2
MS.gene82016.t1 MTR_1g073690 36.250 80 50 1 20 99 2 80 5.19e-14 72.0
MS.gene82016.t1 MTR_4g070080 43.590 78 39 2 25 99 7 82 8.56e-14 68.2
MS.gene82016.t1 MTR_3g084040 40.580 69 41 0 24 92 36 104 9.78e-14 67.4
MS.gene82016.t1 MTR_4g109540 41.026 78 45 1 24 100 42 119 1.99e-13 68.9
MS.gene82016.t1 MTR_3g084040 40.909 66 39 0 24 89 36 101 2.43e-13 65.5
MS.gene82016.t1 MTR_4g063560 45.070 71 38 1 24 93 33 103 3.75e-13 65.1
MS.gene82016.t1 MTR_7g013730 38.961 77 46 1 25 100 41 117 3.96e-13 68.2
MS.gene82016.t1 MTR_4g070140 43.421 76 42 1 25 99 9 84 4.14e-13 67.0
MS.gene82016.t1 MTR_3g025880 39.024 82 41 2 24 100 44 121 8.04e-13 68.6
MS.gene82016.t1 MTR_7g105260 33.333 99 62 2 25 122 33 128 7.83e-12 62.0
MS.gene82016.t1 MTR_7g013740 41.026 78 41 2 25 99 41 116 2.20e-11 61.2
MS.gene82016.t1 MTR_3g080130 34.783 69 41 1 24 92 36 100 2.24e-11 60.8
MS.gene82016.t1 MTR_3g082650 40.909 66 39 0 25 90 19 84 3.07e-11 63.5
MS.gene82016.t1 MTR_4g109540 37.209 86 45 2 24 100 42 127 4.62e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82016.t1 AT2G46780 56.031 257 74 10 1 223 1 252 2.01e-76 236
MS.gene82016.t1 AT2G46780 56.031 257 74 10 1 223 1 252 2.01e-76 236
MS.gene82016.t1 AT1G33470 55.414 157 65 3 19 170 2 158 1.70e-52 172
MS.gene82016.t1 AT1G33470 55.414 157 64 4 20 170 3 159 7.89e-51 167
MS.gene82016.t1 AT1G33470 55.063 158 65 4 19 170 2 159 9.34e-51 167
MS.gene82016.t1 AT1G20880 49.758 207 80 8 18 209 18 215 1.50e-48 162
MS.gene82016.t1 AT1G20880 49.758 207 80 8 18 209 18 215 1.50e-48 162
MS.gene82016.t1 AT1G20880 49.758 207 80 8 18 209 18 215 1.50e-48 162
MS.gene82016.t1 AT1G76460 47.170 212 78 7 18 209 18 215 4.29e-48 162
MS.gene82016.t1 AT1G22910 47.126 174 54 3 20 171 9 166 1.39e-44 151
MS.gene82016.t1 AT1G22910 47.126 174 54 3 20 171 9 166 2.14e-44 151
MS.gene82016.t1 AT1G22910 47.126 174 54 3 20 171 9 166 1.31e-43 152
MS.gene82016.t1 AT1G22330 71.765 85 24 0 18 102 11 95 6.79e-40 140
MS.gene82016.t1 AT1G78260 71.429 84 24 0 18 101 11 94 1.32e-39 140
MS.gene82016.t1 AT1G78260 71.429 84 24 0 18 101 11 94 3.45e-39 138
MS.gene82016.t1 AT3G54770 50.000 130 64 1 9 137 1 130 1.31e-38 136
MS.gene82016.t1 AT3G54770 50.000 130 64 1 9 137 1 130 1.47e-38 136
MS.gene82016.t1 AT5G11412 63.855 83 30 0 20 102 48 130 8.77e-35 124
MS.gene82016.t1 AT3G06970 48.333 120 52 2 20 129 8 127 2.07e-31 119
MS.gene82016.t1 AT5G53680 57.143 84 36 0 20 103 9 92 1.24e-30 113
MS.gene82016.t1 AT5G53720 57.500 80 34 0 24 103 9 88 2.55e-26 99.8
MS.gene82016.t1 AT3G54770 51.020 98 37 1 40 137 1 87 9.98e-26 101
MS.gene82016.t1 AT2G22090 50.602 83 41 0 20 102 100 182 1.08e-19 87.8
MS.gene82016.t1 AT2G22090 50.602 83 41 0 20 102 100 182 1.14e-19 87.8
MS.gene82016.t1 AT5G47620 48.529 68 34 1 25 92 7 73 2.33e-17 82.0
MS.gene82016.t1 AT5G47620 48.529 68 34 1 25 92 7 73 2.33e-17 82.0
MS.gene82016.t1 AT5G47620 48.529 68 34 1 25 92 7 73 2.33e-17 82.0
MS.gene82016.t1 AT5G47620 47.826 69 35 1 24 92 6 73 2.53e-17 82.0
MS.gene82016.t1 AT3G15010 40.816 98 58 0 5 102 56 153 2.98e-17 81.3
MS.gene82016.t1 AT3G15010 40.816 98 58 0 5 102 56 153 2.98e-17 81.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 1.85e-16 79.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 1.85e-16 79.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 1.85e-16 79.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 1.85e-16 79.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 1.85e-16 79.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.00e-16 79.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.00e-16 79.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.00e-16 79.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.00e-16 79.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.19e-16 79.3
MS.gene82016.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.40e-16 79.0
MS.gene82016.t1 AT4G26650 40.278 72 42 1 21 92 9 79 2.80e-16 79.0
MS.gene82016.t1 AT4G26650 40.278 72 42 1 21 92 12 82 2.93e-16 78.6
MS.gene82016.t1 AT3G07810 46.479 71 31 2 25 92 7 73 1.36e-15 77.0
MS.gene82016.t1 AT3G07810 46.479 71 31 2 25 92 7 73 1.37e-15 77.0
MS.gene82016.t1 AT2G41060 40.964 83 49 0 20 102 124 206 1.65e-15 76.6
MS.gene82016.t1 AT2G41060 40.964 83 49 0 20 102 124 206 1.65e-15 76.6
MS.gene82016.t1 AT2G41060 40.964 83 49 0 20 102 124 206 1.65e-15 76.6
MS.gene82016.t1 AT4G13850 44.444 72 36 1 24 95 35 102 1.95e-15 71.6
MS.gene82016.t1 AT4G13850 44.444 72 36 1 24 95 35 102 2.64e-15 71.6
MS.gene82016.t1 AT4G39260 43.421 76 42 1 25 99 7 82 2.67e-15 71.2
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.92e-15 75.9
MS.gene82016.t1 AT4G13850 44.444 72 36 1 24 95 35 102 3.24e-15 71.6
MS.gene82016.t1 AT2G19380 44.444 81 45 0 20 100 404 484 3.33e-15 75.9
MS.gene82016.t1 AT4G13850 44.444 72 36 1 24 95 35 102 3.62e-15 71.6
MS.gene82016.t1 AT3G23830 41.026 78 45 1 24 100 35 112 4.17e-15 71.2
MS.gene82016.t1 AT3G23830 41.026 78 45 1 24 100 35 112 4.17e-15 71.2
MS.gene82016.t1 AT4G39260 43.421 76 42 1 25 99 7 82 4.50e-15 69.7
MS.gene82016.t1 AT4G14300 41.250 80 46 1 20 99 2 80 5.41e-15 74.7
MS.gene82016.t1 AT4G14300 41.250 80 46 1 20 99 2 80 5.41e-15 74.7
MS.gene82016.t1 AT2G22100 44.156 77 43 0 20 96 159 235 6.42e-15 74.3
MS.gene82016.t1 AT4G39260 43.421 76 42 1 25 99 7 82 7.08e-15 71.2
MS.gene82016.t1 AT1G17640 44.444 72 39 1 25 96 67 137 9.42e-15 73.9
MS.gene82016.t1 AT1G17640 44.444 72 39 1 25 96 67 137 9.42e-15 73.9
MS.gene82016.t1 AT1G17640 44.444 72 39 1 25 96 67 137 9.42e-15 73.9
MS.gene82016.t1 AT4G39260 50.943 53 26 0 25 77 7 59 2.04e-14 68.2
MS.gene82016.t1 AT2G21660 42.105 76 43 1 25 99 9 84 2.59e-14 69.3
MS.gene82016.t1 AT2G33410 41.333 75 43 1 25 99 7 80 2.80e-14 72.8
MS.gene82016.t1 AT2G21660 42.105 76 43 1 25 99 9 84 3.67e-14 69.3
MS.gene82016.t1 AT5G61030 43.478 69 35 1 24 92 40 104 2.21e-13 69.3
MS.gene82016.t1 AT5G61030 43.478 69 35 1 24 92 40 104 2.21e-13 69.3
MS.gene82016.t1 AT1G74230 41.558 77 45 0 24 100 34 110 2.29e-13 69.3
MS.gene82016.t1 AT1G18630 39.785 93 49 3 5 92 15 105 4.16e-13 66.2
MS.gene82016.t1 AT3G26420 45.614 57 31 0 25 81 8 64 4.62e-13 67.8
MS.gene82016.t1 AT5G59865 51.020 49 24 0 24 72 26 74 1.82e-11 60.5
MS.gene82016.t1 AT1G58470 39.189 74 41 1 25 94 7 80 2.68e-11 63.5
MS.gene82016.t1 AT1G58470 39.189 74 41 1 25 94 7 80 2.68e-11 63.5
MS.gene82016.t1 AT1G22910 33.065 124 45 3 70 171 8 115 5.05e-11 61.2

Find 79 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCCTTCTGTTGGAGCTTC+TGG 0.055135 8.2:-71344316 MS.gene82016:CDS
CATAGTAAGGGTACAGATTT+TGG 0.182431 8.2:+71344278 None:intergenic
TAACCCTCCAACAAATATTT+TGG 0.186878 8.2:+71345728 None:intergenic
GCTGAGGCAAAACTGGAATT+TGG 0.187376 8.2:+71344197 None:intergenic
AACTGGAATTTGGGTCATTT+GGG 0.205081 8.2:+71344207 None:intergenic
TATTTATCTGACAGATATTC+TGG 0.249082 8.2:-71344768 MS.gene82016:intron
AGTATCCATGGTCATGGTTT+TGG 0.271120 8.2:-71344241 MS.gene82016:CDS
AGCTTCTGGGGCAATGGGTT+TGG 0.272297 8.2:-71344302 MS.gene82016:CDS
CCAAAATGCTGAGGCAAAAC+TGG 0.286123 8.2:+71344190 None:intergenic
AAACTGGAATTTGGGTCATT+TGG 0.293455 8.2:+71344206 None:intergenic
CATGCTCTTATAGCTGCTTC+TGG 0.298266 8.2:+71345447 None:intergenic
ACATTTCCTTATTCAGCATA+TGG 0.317744 8.2:-71344892 MS.gene82016:CDS
ATCCTTCTGTTGGAGCTTCT+GGG 0.321902 8.2:-71344315 MS.gene82016:CDS
AGTTACCCATATGCTGAATA+AGG 0.331681 8.2:+71344886 None:intergenic
GTTATCACTGATAAGTATAC+TGG 0.334320 8.2:-71345636 MS.gene82016:CDS
TGTTGGAGCTTCTGGGGCAA+TGG 0.334769 8.2:-71344308 MS.gene82016:CDS
GCTCTTCTTCCATCAATAAC+TGG 0.337494 8.2:+71345411 None:intergenic
CAGAACTATTACAATGTCTA+TGG 0.345598 8.2:-71344361 MS.gene82016:CDS
AGAATTATTACCAGCACTAA+TGG 0.360267 8.2:+71344123 None:intergenic
GTTGGAGCTTCTGGGGCAAT+GGG 0.372650 8.2:-71344307 MS.gene82016:CDS
AATGGTGTGCAGGAAGGTTT+AGG 0.381511 8.2:-71344993 MS.gene82016:intron
ACTGGGAGATCTAAAGGTTA+TGG 0.382688 8.2:-71345618 MS.gene82016:CDS
CAAAATCTGTACCCTTACTA+TGG 0.391859 8.2:-71344277 MS.gene82016:CDS
CTGAGGCAAAACTGGAATTT+GGG 0.394905 8.2:+71344198 None:intergenic
TAAATGTTGTGTCATTATAT+TGG 0.402817 8.2:+71345751 None:intergenic
ACATTTACCAAAATATTTGT+TGG 0.412578 8.2:-71345735 MS.gene82016:CDS
AATCCATCTCCAGTTATTGA+TGG 0.412922 8.2:-71345420 MS.gene82016:CDS
TATTTGTTGGAGGGTTAGCT+TGG 0.423354 8.2:-71345722 MS.gene82016:CDS
TATTACCAGCACTAATGGTT+GGG 0.433671 8.2:+71344128 None:intergenic
TGTGCATATTGACCATAGTA+AGG 0.433964 8.2:+71344265 None:intergenic
CATTTCCTTATTCAGCATAT+GGG 0.440818 8.2:-71344891 MS.gene82016:intron
TGTACTGCCTAGTGGGTTGA+TGG 0.442879 8.2:+71344914 None:intergenic
TTGTTACCACTCACCATATG+AGG 0.446616 8.2:+71345780 None:intergenic
ATTTGTTGGAGGGTTAGCTT+GGG 0.450733 8.2:-71345721 MS.gene82016:CDS
TCAACAAAGACAACCTCATA+TGG 0.453555 8.2:-71345793 MS.gene82016:CDS
GTACCATCAGTTCCTTATCA+TGG 0.464112 8.2:-71344955 MS.gene82016:CDS
TCACCTTACTATCCTTCTGT+TGG 0.471067 8.2:-71344325 MS.gene82016:CDS
GCTCCAACAGAAGGATAGTA+AGG 0.482209 8.2:+71344322 None:intergenic
AAGTATACTGGGAGATCTAA+AGG 0.490906 8.2:-71345624 MS.gene82016:CDS
CTTCCATCAATAACTGGAGA+TGG 0.499114 8.2:+71345417 None:intergenic
GCTGGTCCTTCACAAACCTC+TGG 0.505996 8.2:-71343624 MS.gene82016:CDS
AATTGCAATATTGCTTCACT+TGG 0.510571 8.2:-71345387 MS.gene82016:CDS
TTATTACCAGCACTAATGGT+TGG 0.519138 8.2:+71344127 None:intergenic
CCAGCCCTTGTTACTGCCAC+AGG 0.522193 8.2:+71343642 None:intergenic
TTTACCAAAATATTTGTTGG+AGG 0.522261 8.2:-71345732 MS.gene82016:CDS
TCCTTCTGTTGGAGCTTCTG+GGG 0.522825 8.2:-71344314 MS.gene82016:CDS
CAGCACCTGTGGCAGTAACA+AGG 0.532377 8.2:-71343647 MS.gene82016:CDS
GGTTAGCTTGGGAAACTCAA+AGG 0.538530 8.2:-71345710 MS.gene82016:CDS
AGGAAATGTGTACTGCCTAG+TGG 0.539088 8.2:+71344906 None:intergenic
TGCTGCTATTGTAGTAGTTG+TGG 0.540042 8.2:+71343665 None:intergenic
TTGTTCAGTATCTGTTCCAG+AGG 0.543034 8.2:+71343608 None:intergenic
GTGGATGAAGAACCATGATA+AGG 0.546300 8.2:+71344943 None:intergenic
TTACCAAAATATTTGTTGGA+GGG 0.548943 8.2:-71345731 MS.gene82016:CDS
GTTGGGATTGCAACTGAAGA+AGG 0.551412 8.2:+71344145 None:intergenic
CAATATGCACAAAGTATCCA+TGG 0.551625 8.2:-71344253 MS.gene82016:CDS
CCTGTGGCAGTAACAAGGGC+TGG 0.554680 8.2:-71343642 MS.gene82016:CDS
GCAATCCCAACCATTAGTGC+TGG 0.558559 8.2:-71344133 MS.gene82016:intron
CTGGAATTTGGGTCATTTGG+GGG 0.562855 8.2:+71344209 None:intergenic
GCCCCAGAAGCTCCAACAGA+AGG 0.575329 8.2:+71344313 None:intergenic
GCACAAAGTATCCATGGTCA+TGG 0.581950 8.2:-71344247 MS.gene82016:CDS
GAACCATGATAAGGAACTGA+TGG 0.583714 8.2:+71344952 None:intergenic
ACTGGAATTTGGGTCATTTG+GGG 0.588666 8.2:+71344208 None:intergenic
GTTAGCTTGGGAAACTCAAA+GGG 0.597037 8.2:-71345709 MS.gene82016:CDS
GTGCATATTGACCATAGTAA+GGG 0.598572 8.2:+71344266 None:intergenic
AGCACCTGTGGCAGTAACAA+GGG 0.600833 8.2:-71343646 MS.gene82016:CDS
TACTACAATAGCAGCACCTG+TGG 0.605257 8.2:-71343658 MS.gene82016:CDS
TTATCACTGATAAGTATACT+GGG 0.624818 8.2:-71345635 MS.gene82016:CDS
TCTGTTCCAGAGGTTTGTGA+AGG 0.625089 8.2:+71343618 None:intergenic
CGATCACTAACTCTTCAGCA+TGG 0.635608 8.2:-71345351 MS.gene82016:intron
GAACTCCAAAACCATGACCA+TGG 0.639031 8.2:+71344236 None:intergenic
GGAAGGTTTAGGCCTCCACA+TGG 0.645450 8.2:-71344982 MS.gene82016:CDS
GGAAATGTGTACTGCCTAGT+GGG 0.657310 8.2:+71344907 None:intergenic
AGACAACCTCATATGGTGAG+TGG 0.661054 8.2:-71345786 MS.gene82016:CDS
CCTTGTGACCCAAAATGCTG+AGG 0.666097 8.2:+71344181 None:intergenic
GTTTAGGCCTCCACATGGAG+TGG 0.666440 8.2:-71344977 MS.gene82016:CDS
GATGGTACCACTCCATGTGG+AGG 0.680340 8.2:+71344970 None:intergenic
ACTGATGGTACCACTCCATG+TGG 0.705430 8.2:+71344967 None:intergenic
ACAAGACACATTGTATCCTG+CGG 0.715658 8.2:-71344739 MS.gene82016:intron
AGTGGGTTGATGGAAAACTG+TGG 0.742505 8.2:+71344924 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAAAAAAAAAAAAA+AGG + chr8.2:71344147-71344166 None:intergenic 0.0%
!!! TTTTTTTTTTTTAAAAAAAA+AGG - chr8.2:71344149-71344168 MS.gene82016:CDS 0.0%
!!! AATTTTTTTTTTGTTTTTGT+AGG - chr8.2:71343904-71343923 MS.gene82016:intron 10.0%
!!! TATGTTTTATAAGATTTTAA+AGG - chr8.2:71344363-71344382 MS.gene82016:intron 10.0%
!! AAATATAAGGAAAAGAAAAA+TGG + chr8.2:71343806-71343825 None:intergenic 15.0%
!! AGCATAGTAAAATAAATATA+AGG + chr8.2:71343819-71343838 None:intergenic 15.0%
!! ATTAATTTAGTATCAAAACT+AGG - chr8.2:71345373-71345392 MS.gene82016:CDS 15.0%
!! GTAGTTTCTTTAATAAAAAA+AGG + chr8.2:71344260-71344279 None:intergenic 15.0%
!! TTTGGATTAATAGTAAAAAT+CGG - chr8.2:71344700-71344719 MS.gene82016:intron 15.0%
!!! AGAATTTATTTTGAACAATT+TGG - chr8.2:71343710-71343729 MS.gene82016:intron 15.0%
!!! TTTTAAAGGAAAAATTGAAA+TGG - chr8.2:71344377-71344396 MS.gene82016:intron 15.0%
!! AAATAATCAACCATAAAAAG+TGG + chr8.2:71344282-71344301 None:intergenic 20.0%
!! AAATTAATCACATACATGTT+TGG + chr8.2:71344824-71344843 None:intergenic 20.0%
!! AAGAAAGAAATAAAAGAGAA+TGG + chr8.2:71344077-71344096 None:intergenic 20.0%
!! ACATTTACCAAAATATTTGT+TGG - chr8.2:71343653-71343672 MS.gene82016:CDS 20.0%
!! GTTTCTTTAATAAAAAAAGG+TGG + chr8.2:71344257-71344276 None:intergenic 20.0%
!!! GGTATATTGTGTTATTTATT+TGG - chr8.2:71344170-71344189 MS.gene82016:CDS 20.0%
!!! TAAATGTTGTGTCATTATAT+TGG + chr8.2:71343640-71343659 None:intergenic 20.0%
! AAATAAAAGAGAATGGTAGA+AGG + chr8.2:71344070-71344089 None:intergenic 25.0%
! TATTTATCTGACAGATATTC+TGG - chr8.2:71344620-71344639 MS.gene82016:intron 25.0%
! TGAGCAATTCTATTTCAAAA+TGG - chr8.2:71345336-71345355 MS.gene82016:intron 25.0%
! TGTAATAAATTCCACATGTT+TGG - chr8.2:71344682-71344701 MS.gene82016:intron 25.0%
! TTACTATTAATCCAAACATG+TGG + chr8.2:71344696-71344715 None:intergenic 25.0%
! TTATCACTGATAAGTATACT+GGG - chr8.2:71343753-71343772 MS.gene82016:intron 25.0%
! TTCATTATGTATTCGTTGTA+AGG - chr8.2:71345456-71345475 MS.gene82016:CDS 25.0%
!! TTACCAAAATATTTGTTGGA+GGG - chr8.2:71343657-71343676 MS.gene82016:CDS 25.0%
!! TTTACCAAAATATTTGTTGG+AGG - chr8.2:71343656-71343675 MS.gene82016:CDS 25.0%
!!! AAAGAAACTACCACTTTTTA+TGG - chr8.2:71344269-71344288 MS.gene82016:CDS 25.0%
!!! ATCATATTAGTCTTTTCAAG+TGG + chr8.2:71345545-71345564 None:intergenic 25.0%
!!! TCATATTAGTCTTTTCAAGT+GGG + chr8.2:71345544-71345563 None:intergenic 25.0%
AATTGCAATATTGCTTCACT+TGG - chr8.2:71344001-71344020 MS.gene82016:intron 30.0%
ACATTTCCTTATTCAGCATA+TGG - chr8.2:71344496-71344515 MS.gene82016:intron 30.0%
AGAATTATTACCAGCACTAA+TGG + chr8.2:71345268-71345287 None:intergenic 30.0%
CAGAACTATTACAATGTCTA+TGG - chr8.2:71345027-71345046 MS.gene82016:intron 30.0%
CATTTCCTTATTCAGCATAT+GGG - chr8.2:71344497-71344516 MS.gene82016:intron 30.0%
CTAGGATGAATCAAATTGAT+AGG + chr8.2:71344223-71344242 None:intergenic 30.0%
CTATCAATTTGATTCATCCT+AGG - chr8.2:71344221-71344240 MS.gene82016:CDS 30.0%
GTTATCACTGATAAGTATAC+TGG - chr8.2:71343752-71343771 MS.gene82016:intron 30.0%
TAGAATTGCCATCAAAGAAA+AGG + chr8.2:71344597-71344616 None:intergenic 30.0%
! ACAATGACACAGTAATTTTG+TGG - chr8.2:71344730-71344749 MS.gene82016:intron 30.0%
! CAAATTGATAGGTTGAACAA+TGG + chr8.2:71344212-71344231 None:intergenic 30.0%
! GTTTTTGTAGGTGACATTTA+AGG - chr8.2:71343916-71343935 MS.gene82016:intron 30.0%
! TAACCCTCCAACAAATATTT+TGG + chr8.2:71343663-71343682 None:intergenic 30.0%
AAACTGGAATTTGGGTCATT+TGG + chr8.2:71345185-71345204 None:intergenic 35.0%
AACTGGAATTTGGGTCATTT+GGG + chr8.2:71345184-71345203 None:intergenic 35.0%
AAGTATACTGGGAGATCTAA+AGG - chr8.2:71343764-71343783 MS.gene82016:intron 35.0%
AATCCATCTCCAGTTATTGA+TGG - chr8.2:71343968-71343987 MS.gene82016:intron 35.0%
AGAATCATAGTATGTCACAG+AGG - chr8.2:71344893-71344912 MS.gene82016:CDS 35.0%
AGTTACCCATATGCTGAATA+AGG + chr8.2:71344505-71344524 None:intergenic 35.0%
CAAAATCTGTACCCTTACTA+TGG - chr8.2:71345111-71345130 MS.gene82016:intron 35.0%
CACATACATGTTTGGATTGA+CGG + chr8.2:71344816-71344835 None:intergenic 35.0%
GAAAAATTGAAATGGTGTGC+AGG - chr8.2:71344385-71344404 MS.gene82016:intron 35.0%
GTGCATATTGACCATAGTAA+GGG + chr8.2:71345125-71345144 None:intergenic 35.0%
TATTACCAGCACTAATGGTT+GGG + chr8.2:71345263-71345282 None:intergenic 35.0%
TCAACAAAGACAACCTCATA+TGG - chr8.2:71343595-71343614 MS.gene82016:CDS 35.0%
TGTGCATATTGACCATAGTA+AGG + chr8.2:71345126-71345145 None:intergenic 35.0%
TTATTACCAGCACTAATGGT+TGG + chr8.2:71345264-71345283 None:intergenic 35.0%
! CAATATGCACAAAGTATCCA+TGG - chr8.2:71345135-71345154 MS.gene82016:intron 35.0%
! TTGTCTGTCCTTTTCTTTGA+TGG - chr8.2:71344586-71344605 MS.gene82016:intron 35.0%
!! CATAGTAAGGGTACAGATTT+TGG + chr8.2:71345113-71345132 None:intergenic 35.0%
AATTGAAATGGTGTGCAGGA+AGG - chr8.2:71344389-71344408 MS.gene82016:intron 40.0%
ACAAGACACATTGTATCCTG+CGG - chr8.2:71344649-71344668 MS.gene82016:intron 40.0%
ACTGGAATTTGGGTCATTTG+GGG + chr8.2:71345183-71345202 None:intergenic 40.0%
ACTGGGAGATCTAAAGGTTA+TGG - chr8.2:71343770-71343789 MS.gene82016:intron 40.0%
ACTTCGACCAAGTGTATGTT+TGG + chr8.2:71344940-71344959 None:intergenic 40.0%
AGTGACTCCAAACATACACT+TGG - chr8.2:71344930-71344949 MS.gene82016:CDS 40.0%
ATTACATCAACACTTACCGC+AGG + chr8.2:71344668-71344687 None:intergenic 40.0%
CTGAGGCAAAACTGGAATTT+GGG + chr8.2:71345193-71345212 None:intergenic 40.0%
CTTCCATCAATAACTGGAGA+TGG + chr8.2:71343974-71343993 None:intergenic 40.0%
GAACCATGATAAGGAACTGA+TGG + chr8.2:71344439-71344458 None:intergenic 40.0%
GCTCTTCTTCCATCAATAAC+TGG + chr8.2:71343980-71343999 None:intergenic 40.0%
GTACCATCAGTTCCTTATCA+TGG - chr8.2:71344433-71344452 MS.gene82016:intron 40.0%
GTGGATGAAGAACCATGATA+AGG + chr8.2:71344448-71344467 None:intergenic 40.0%
TCACCTTACTATCCTTCTGT+TGG - chr8.2:71345063-71345082 MS.gene82016:intron 40.0%
TGCTGCTATTGTAGTAGTTG+TGG + chr8.2:71345726-71345745 None:intergenic 40.0%
TTGTTACCACTCACCATATG+AGG + chr8.2:71343611-71343630 None:intergenic 40.0%
TTGTTCAGTATCTGTTCCAG+AGG + chr8.2:71345783-71345802 None:intergenic 40.0%
! AGTATCCATGGTCATGGTTT+TGG - chr8.2:71345147-71345166 MS.gene82016:intron 40.0%
! CAGTTTTGCCTCAGCATTTT+GGG - chr8.2:71345199-71345218 MS.gene82016:intron 40.0%
! GTTAGCTTGGGAAACTCAAA+GGG - chr8.2:71343679-71343698 MS.gene82016:CDS 40.0%
! TGATAGGTTGAACAATGGCA+GGG + chr8.2:71344207-71344226 None:intergenic 40.0%
! TTGATAGGTTGAACAATGGC+AGG + chr8.2:71344208-71344227 None:intergenic 40.0%
!! ATTTGTTGGAGGGTTAGCTT+GGG - chr8.2:71343667-71343686 MS.gene82016:CDS 40.0%
!! TATTTGTTGGAGGGTTAGCT+TGG - chr8.2:71343666-71343685 MS.gene82016:CDS 40.0%
AATGGTGTGCAGGAAGGTTT+AGG - chr8.2:71344395-71344414 MS.gene82016:intron 45.0%
AGACAACCTCATATGGTGAG+TGG - chr8.2:71343602-71343621 MS.gene82016:CDS 45.0%
AGGAAATGTGTACTGCCTAG+TGG + chr8.2:71344485-71344504 None:intergenic 45.0%
ATCCTTCTGTTGGAGCTTCT+GGG - chr8.2:71345073-71345092 MS.gene82016:intron 45.0%
CATGCTCTTATAGCTGCTTC+TGG + chr8.2:71343944-71343963 None:intergenic 45.0%
CCAAAATGCTGAGGCAAAAC+TGG + chr8.2:71345201-71345220 None:intergenic 45.0%
CGATCACTAACTCTTCAGCA+TGG - chr8.2:71344037-71344056 MS.gene82016:intron 45.0%
CTGGAATTTGGGTCATTTGG+GGG + chr8.2:71345182-71345201 None:intergenic 45.0%
GAACTCCAAAACCATGACCA+TGG + chr8.2:71345155-71345174 None:intergenic 45.0%
GCTCCAACAGAAGGATAGTA+AGG + chr8.2:71345069-71345088 None:intergenic 45.0%
GCTGAGGCAAAACTGGAATT+TGG + chr8.2:71345194-71345213 None:intergenic 45.0%
GGAAATGTGTACTGCCTAGT+GGG + chr8.2:71344484-71344503 None:intergenic 45.0%
GTTGGGATTGCAACTGAAGA+AGG + chr8.2:71345246-71345265 None:intergenic 45.0%
TACTACAATAGCAGCACCTG+TGG - chr8.2:71345730-71345749 MS.gene82016:CDS 45.0%
TATCCTTCTGTTGGAGCTTC+TGG - chr8.2:71345072-71345091 MS.gene82016:intron 45.0%
TCTGTTCCAGAGGTTTGTGA+AGG + chr8.2:71345773-71345792 None:intergenic 45.0%
! AAGGTGGTAGCTTTTAGCCT+AGG + chr8.2:71344241-71344260 None:intergenic 45.0%
! CAGTTTTCCATCAACCCACT+AGG - chr8.2:71344467-71344486 MS.gene82016:intron 45.0%
! CCAGTTTTGCCTCAGCATTT+TGG - chr8.2:71345198-71345217 MS.gene82016:intron 45.0%
! GCACAAAGTATCCATGGTCA+TGG - chr8.2:71345141-71345160 MS.gene82016:intron 45.0%
!! AGTGGGTTGATGGAAAACTG+TGG + chr8.2:71344467-71344486 None:intergenic 45.0%
!! GGTTAGCTTGGGAAACTCAA+AGG - chr8.2:71343678-71343697 MS.gene82016:CDS 45.0%
!!! TTTATTTACTATTTTTTTTT+TGG - chr8.2:71344299-71344318 MS.gene82016:CDS 5.0%
ACTGATGGTACCACTCCATG+TGG + chr8.2:71344424-71344443 None:intergenic 50.0%
AGCACCTGTGGCAGTAACAA+GGG - chr8.2:71345742-71345761 MS.gene82016:CDS 50.0%
CCTTGTGACCCAAAATGCTG+AGG + chr8.2:71345210-71345229 None:intergenic 50.0%
GCAATCCCAACCATTAGTGC+TGG - chr8.2:71345255-71345274 MS.gene82016:intron 50.0%
TCCTTCTGTTGGAGCTTCTG+GGG - chr8.2:71345074-71345093 MS.gene82016:intron 50.0%
!! CCTCAGCATTTTGGGTCACA+AGG - chr8.2:71345207-71345226 MS.gene82016:intron 50.0%
!! TGTACTGCCTAGTGGGTTGA+TGG + chr8.2:71344477-71344496 None:intergenic 50.0%
CAGCACCTGTGGCAGTAACA+AGG - chr8.2:71345741-71345760 MS.gene82016:CDS 55.0%
GATGGTACCACTCCATGTGG+AGG + chr8.2:71344421-71344440 None:intergenic 55.0%
GCTGGTCCTTCACAAACCTC+TGG - chr8.2:71345764-71345783 MS.gene82016:CDS 55.0%
GGAAGGTTTAGGCCTCCACA+TGG - chr8.2:71344406-71344425 MS.gene82016:intron 55.0%
GTTTAGGCCTCCACATGGAG+TGG - chr8.2:71344411-71344430 MS.gene82016:intron 55.0%
!! AGCTTCTGGGGCAATGGGTT+TGG - chr8.2:71345086-71345105 MS.gene82016:intron 55.0%
!! GTTGGAGCTTCTGGGGCAAT+GGG - chr8.2:71345081-71345100 MS.gene82016:intron 55.0%
!! TGTTGGAGCTTCTGGGGCAA+TGG - chr8.2:71345080-71345099 MS.gene82016:intron 55.0%
CCAGCCCTTGTTACTGCCAC+AGG + chr8.2:71345749-71345768 None:intergenic 60.0%
CCTGTGGCAGTAACAAGGGC+TGG - chr8.2:71345746-71345765 MS.gene82016:CDS 60.0%
GCCCCAGAAGCTCCAACAGA+AGG + chr8.2:71345078-71345097 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 71343590 71345820 71343590 ID=MS.gene82016
chr8.2 mRNA 71343590 71345820 71343590 ID=MS.gene82016.t1;Parent=MS.gene82016
chr8.2 exon 71345614 71345820 71345614 ID=MS.gene82016.t1.exon1;Parent=MS.gene82016.t1
chr8.2 CDS 71345614 71345820 71345614 ID=cds.MS.gene82016.t1;Parent=MS.gene82016.t1
chr8.2 exon 71345352 71345484 71345352 ID=MS.gene82016.t1.exon2;Parent=MS.gene82016.t1
chr8.2 CDS 71345352 71345484 71345352 ID=cds.MS.gene82016.t1;Parent=MS.gene82016.t1
chr8.2 exon 71344892 71345003 71344892 ID=MS.gene82016.t1.exon3;Parent=MS.gene82016.t1
chr8.2 CDS 71344892 71345003 71344892 ID=cds.MS.gene82016.t1;Parent=MS.gene82016.t1
chr8.2 exon 71344740 71344776 71344740 ID=MS.gene82016.t1.exon4;Parent=MS.gene82016.t1
chr8.2 CDS 71344740 71344776 71344740 ID=cds.MS.gene82016.t1;Parent=MS.gene82016.t1
chr8.2 exon 71344134 71344380 71344134 ID=MS.gene82016.t1.exon5;Parent=MS.gene82016.t1
chr8.2 CDS 71344134 71344380 71344134 ID=cds.MS.gene82016.t1;Parent=MS.gene82016.t1
chr8.2 exon 71343590 71343702 71343590 ID=MS.gene82016.t1.exon6;Parent=MS.gene82016.t1
chr8.2 CDS 71343590 71343702 71343590 ID=cds.MS.gene82016.t1;Parent=MS.gene82016.t1
Gene Sequence

>MS.gene82016

ATGTCTCAACAAAGACAACCTCATATGGTGAGTGGTAACAATCAAGACCAATATAATGACACAACATTTACCAAAATATTTGTTGGAGGGTTAGCTTGGGAAACTCAAAGGGAAACAATGAGAATTTATTTTGAACAATTTGGAGAGATCTTAGAAGCTGTTGTTATCACTGATAAGTATACTGGGAGATCTAAAGGTTATGGTTTTGTAAGTCCATTTTTCTTTTCCTTATATTTATTTTACTATGCTTCTCACATTTGTGCATAGTTTATATTTTACTTATATATTTATATGATCTTAGACAATGATTTTGCAATTTTTTTTTTGTTTTTGTAGGTGACATTTAAGGATCCAGAAGCAGCTATAAGAGCATGTCAAAATCCATCTCCAGTTATTGATGGAAGAAGAGCAAATTGCAATATTGCTTCACTTGGTGCTAACAAGAATCGATCACTAACTCTTCAGCATGGTTTTAATCCTTCTACCATTCTCTTTTATTTCTTTCTTTTTTTTTTTTTACTTGTTATACTATGATCAAATTTATAGTAAAATATCCTTTTTTTTTTTTTTTAAAAAAAAAGGTATATTGTGTTATTTATTTGGTTTTTAATTTTCCCTGCCATTGTTCAACCTATCAATTTGATTCATCCTAGGCTAAAAGCTACCACCTTTTTTTATTAAAGAAACTACCACTTTTTATGGTTGATTATTTATTTACTATTTTTTTTTTGGTACAAATTTACTAATTTATTTGTTGAAAAAAAATATGATTATATGTTTTATAAGATTTTAAAGGAAAAATTGAAATGGTGTGCAGGAAGGTTTAGGCCTCCACATGGAGTGGTACCATCAGTTCCTTATCATGGTTCTTCATCCACAGTTTTCCATCAACCCACTAGGCAGTACACATTTCCTTATTCAGCATATGGGTAACTTTCATGTTATGAACATTTTTATGTTGAGCTAGTTATTTTTTTATGAACATTTGTATATTCTTTGTCTGTCCTTTTCTTTGATGGCAATTCTATGATATTTATCTGACAGATATTCTGGATATTCACAAGACACATTGTATCCTGCGGTAAGTGTTGATGTAATAAATTCCACATGTTTGGATTAATAGTAAAAATCGGAAAAATCACAATGACACAGTAATTTTGTGGAAACTTGAAAGTGTATTTTTGTCAAAATCACGTTGTTTTATCGTAATTATGTGAATTTCACCGTCAATCCAAACATGTATGTGATTAATTTGAAAGAATCATAGTATGTCATTGTCATTTTGACAAAAACTACATTTTAAAGAATCATAGTATGTCACAGAGGCATCGTCATTTCGCAGTGACTCCAAACATACACTTGGTCGAAGTAAACACATAAATTATAATTTTGATACATTATTATTTATTATTGTGTACTCTTTTGTGTAAACAATTGCAGAACTATTACAATGTCTATGGAGTTCAACAATATTCACCTTACTATCCTTCTGTTGGAGCTTCTGGGGCAATGGGTTTGGTCCAAAATCTGTACCCTTACTATGGTCAATATGCACAAAGTATCCATGGTCATGGTTTTGGAGTTCAATACCCCCAAATGACCCAAATTCCAGTTTTGCCTCAGCATTTTGGGTCACAAGGAATATTGACTCTTCCTTCTTCAGTTGCAATCCCAACCATTAGTGCTGGTAATAATTCTAATACATTATGATATTATTTTTAAATTTTTATAATTTCTCTTCATTGTTGAGCAATTCTATTTCAAAATGGAGTTAGAATCACTAATTAATTTAGTATCAAAACTAGGCATATAAAATTTGATCACGAAATTTCAAAATGATTCATTTAGTAAAAAACGAAAGAATAGTTCATTATGTATTCGTTGTAAGGCTGCTTATTTAAAAAAAAATATATATATATATATACACACACACACTTAGAAACTAAATCGACCCACTTGAAAAGACTAATATGATCAAGAATTTTCAATTTTAAGAATTGTTTTGAAACTTCATTAATGTCAAATATCAATTTATGTGAACTCTATAATTTGAGAGATTAAATTAGTGATTTCATCATTTTAATTTTGTATTGAAGACTAAATTGTAACAATTTTCAGCAACAGTAACTGCAACCACAACTACTACAATAGCAGCACCTGTGGCAGTAACAAGGGCTGGTCCTTCACAAACCTCTGGAACAGATACTGAACAACAACATTCTACATCTTGA
Protein sequence

>MS.gene82016.t1

MSQQRQPHMVSGNNQDQYNDTTFTKIFVGGLAWETQRETMRIYFEQFGEILEAVVITDKYTGRSKGYGFVTFKDPEAAIRACQNPSPVIDGRRANCNIASLGANKNRSLTLQHGRFRPPHGVVPSVPYHGSSSTVFHQPTRQYTFPYSAYGYSGYSQDTLYPANYYNVYGVQQYSPYYPSVGASGAMGLVQNLYPYYGQYAQSIHGHGFGVQYPQMTQIPVLPQHFGSQGILTLPSSVAIPTISAATVTATTTTTIAAPVAVTRAGPSQTSGTDTEQQHSTS