AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82233

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82233.t1 MTR_4g028960 96.444 703 23 1 1 703 1 701 0.0 1384
MS.gene82233.t1 MTR_2g018010 37.054 672 383 15 20 667 20 675 6.13e-121 378
MS.gene82233.t1 MTR_5g083030 30.962 717 406 17 1 667 8 685 4.28e-91 298
MS.gene82233.t1 MTR_4g107010 29.928 695 410 19 7 668 16 666 8.39e-82 275
MS.gene82233.t1 MTR_3g078160 29.446 686 429 15 9 668 3 659 3.52e-80 269
MS.gene82233.t1 MTR_3g078160 29.446 686 429 15 9 668 3 659 3.95e-80 269
MS.gene82233.t1 MTR_3g078160 32.130 554 328 12 9 543 3 527 1.53e-73 248
MS.gene82233.t1 MTR_1g094215 31.554 637 398 16 38 667 15 620 1.83e-71 244
MS.gene82233.t1 MTR_4g485520 33.168 606 325 23 95 667 90 648 6.32e-66 230
MS.gene82233.t1 MTR_4g485520 38.861 404 205 13 280 667 103 480 6.05e-65 223
MS.gene82233.t1 MTR_1g094215 38.520 392 219 10 281 667 63 437 2.72e-62 215
MS.gene82233.t1 MTR_4g063800 30.294 647 410 19 35 667 9 628 3.17e-62 220
MS.gene82233.t1 MTR_7g078330 31.570 586 360 16 95 667 85 642 2.37e-61 217
MS.gene82233.t1 MTR_5g034440 27.273 693 434 18 7 668 14 667 6.56e-59 211
MS.gene82233.t1 MTR_7g059405 37.113 388 223 10 281 665 263 632 1.68e-58 209
MS.gene82233.t1 MTR_7g053260 32.905 389 241 10 283 667 79 451 2.60e-49 179
MS.gene82233.t1 MTR_7g116600 32.648 389 243 9 283 667 79 452 2.22e-47 174
MS.gene82233.t1 MTR_1g079450 31.234 397 249 10 273 662 54 433 5.74e-41 155
MS.gene82233.t1 MTR_3g085610 27.002 437 256 10 283 666 281 707 9.31e-38 150
MS.gene82233.t1 MTR_8g080280 26.185 443 254 10 283 667 283 710 3.75e-36 145
MS.gene82233.t1 MTR_2g011140 29.504 383 244 8 287 666 20 379 7.57e-30 122
MS.gene82233.t1 MTR_4g085720 26.415 424 254 14 282 680 14 404 8.34e-26 110
MS.gene82233.t1 MTR_4g091880 27.692 390 250 8 282 666 5 367 8.60e-26 110
MS.gene82233.t1 MTR_8g092870 26.087 414 260 15 278 666 6 398 1.01e-25 110
MS.gene82233.t1 MTR_4g091880 29.412 391 214 15 282 666 5 339 7.59e-24 103
MS.gene82233.t1 MTR_7g097020 33.117 308 181 10 376 674 472 763 9.04e-24 107
MS.gene82233.t1 MTR_7g097020 59.420 69 28 0 286 354 245 313 2.64e-23 105
MS.gene82233.t1 MTR_3g466220 55.952 84 36 1 272 354 222 305 9.50e-24 107
MS.gene82233.t1 MTR_3g466220 35.793 271 160 8 408 674 574 834 7.22e-22 101
MS.gene82233.t1 MTR_3g466220 55.952 84 36 1 272 354 201 284 1.03e-23 107
MS.gene82233.t1 MTR_3g466220 35.793 271 160 8 408 674 553 813 1.03e-21 100
MS.gene82233.t1 MTR_3g065080 26.220 328 185 12 279 561 33 348 1.84e-23 104
MS.gene82233.t1 MTR_1g069845 62.687 67 25 0 283 349 125 191 2.35e-23 105
MS.gene82233.t1 MTR_1g069845 31.908 304 184 11 380 674 317 606 6.78e-21 97.8
MS.gene82233.t1 MTR_1g069845 62.687 67 25 0 283 349 246 312 5.42e-23 104
MS.gene82233.t1 MTR_1g069845 32.119 302 186 11 380 674 438 727 3.42e-21 99.0
MS.gene82233.t1 MTR_1g069845 62.687 67 25 0 283 349 242 308 5.93e-23 104
MS.gene82233.t1 MTR_1g069845 31.788 302 187 10 380 674 434 723 3.77e-21 99.0
MS.gene82233.t1 MTR_5g048050 51.948 77 37 0 277 353 20 96 1.75e-22 101
MS.gene82233.t1 MTR_5g020570 23.128 454 278 12 275 666 281 725 2.82e-22 102
MS.gene82233.t1 MTR_2g087350 32.710 214 120 7 279 486 3 198 3.67e-22 100
MS.gene82233.t1 MTR_6g071340 51.316 76 37 0 278 353 1 76 4.36e-22 99.8
MS.gene82233.t1 MTR_5g020570 23.128 454 278 12 275 666 266 710 4.38e-22 102
MS.gene82233.t1 MTR_1g093965 29.834 181 95 5 295 470 1 154 6.94e-22 94.7
MS.gene82233.t1 MTR_8g077205 21.105 507 307 11 229 666 114 596 8.33e-22 100
MS.gene82233.t1 MTR_5g077510 47.368 76 40 0 278 353 31 106 9.07e-22 99.4
MS.gene82233.t1 MTR_8g077205 21.105 507 307 11 229 666 226 708 1.27e-21 100
MS.gene82233.t1 MTR_3g103050 30.182 275 183 5 397 667 61 330 3.47e-21 95.9
MS.gene82233.t1 MTR_3g095730 25.899 417 255 17 278 666 9 399 2.16e-20 94.7
MS.gene82233.t1 MTR_7g106340 29.276 304 169 13 279 559 5 285 2.56e-20 94.7
MS.gene82233.t1 MTR_1g093995 29.282 181 96 5 295 470 1 154 2.59e-20 90.1
MS.gene82233.t1 MTR_1g018180 32.500 280 164 8 398 667 72 336 1.39e-19 91.3
MS.gene82233.t1 MTR_3g096370 51.429 70 34 0 279 348 7 76 1.56e-19 92.0
MS.gene82233.t1 MTR_1g056840 27.190 331 165 14 275 559 2 302 3.29e-19 91.3
MS.gene82233.t1 MTR_5g015210 25.745 369 224 11 276 628 60 394 9.18e-19 90.1
MS.gene82233.t1 MTR_1g056880 24.605 443 251 17 275 664 2 414 1.10e-18 89.7
MS.gene82233.t1 MTR_1g056870 36.471 170 72 8 275 432 2 147 1.83e-17 85.9
MS.gene82233.t1 MTR_1g017770 31.081 222 119 8 282 498 45 237 2.32e-17 85.5
MS.gene82233.t1 MTR_2g026130 32.258 279 144 10 408 666 196 449 1.28e-16 83.6
MS.gene82233.t1 MTR_1g056910 27.000 300 172 13 279 559 3 274 2.80e-16 82.0
MS.gene82233.t1 MTR_1g100820 50.000 66 33 0 283 348 649 714 9.45e-16 81.6
MS.gene82233.t1 MTR_1g100820 50.000 66 33 0 283 348 647 712 9.51e-16 81.6
MS.gene82233.t1 MTR_8g039560 30.128 312 182 12 375 665 1 297 3.17e-15 78.2
MS.gene82233.t1 MTR_7g117890 27.907 258 150 8 109 348 82 321 3.18e-15 80.5
MS.gene82233.t1 MTR_7g077780 48.529 68 35 0 283 350 827 894 6.74e-15 79.3
MS.gene82233.t1 MTR_4g051515 34.810 158 84 7 279 433 3 144 1.02e-14 77.4
MS.gene82233.t1 MTR_8g027140 22.489 458 292 15 109 550 83 493 1.82e-14 77.8
MS.gene82233.t1 MTR_4g125930 46.377 69 36 1 283 351 492 559 3.15e-13 73.2
MS.gene82233.t1 MTR_4g125930 46.377 69 36 1 283 351 692 759 4.19e-13 73.2
MS.gene82233.t1 MTR_3g115670 24.936 389 218 14 264 584 13 395 4.83e-13 73.2
MS.gene82233.t1 MTR_5g032010 44.776 67 37 0 282 348 732 798 1.04e-12 72.0
MS.gene82233.t1 MTR_2g096850 44.000 75 41 1 277 350 735 809 1.18e-12 72.0
MS.gene82233.t1 MTR_2g096850 44.000 75 41 1 277 350 584 658 1.26e-12 71.6
MS.gene82233.t1 MTR_1g090320 43.056 72 37 3 283 352 514 583 1.99e-11 68.2
MS.gene82233.t1 MTR_1g090320 43.056 72 37 3 283 352 516 585 2.01e-11 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82233.t1 AT5G01830 53.266 689 275 11 20 701 21 669 0.0 686
MS.gene82233.t1 AT1G29340 35.474 623 360 11 106 696 115 727 2.82e-110 350
MS.gene82233.t1 AT1G60190 30.303 693 411 21 7 668 14 665 2.62e-77 261
MS.gene82233.t1 AT3G54850 30.793 656 398 20 27 667 8 622 2.68e-71 244
MS.gene82233.t1 AT5G42340 31.469 572 346 16 109 667 118 656 1.33e-66 232
MS.gene82233.t1 AT3G46510 28.702 655 416 15 33 669 10 631 1.70e-66 232
MS.gene82233.t1 AT1G10560 28.307 703 423 21 7 668 14 676 8.14e-66 231
MS.gene82233.t1 AT1G23030 31.190 622 353 16 66 667 43 609 1.45e-65 228
MS.gene82233.t1 AT2G28830 30.159 567 363 13 119 668 13 563 2.62e-59 210
MS.gene82233.t1 AT1G71020 30.211 662 370 18 42 667 15 620 3.79e-59 211
MS.gene82233.t1 AT1G71020 33.676 487 269 13 207 667 14 472 5.27e-59 207
MS.gene82233.t1 AT3G07360 32.927 410 246 13 271 667 59 452 7.15e-48 176
MS.gene82233.t1 AT1G67530 26.912 680 375 23 90 666 46 706 1.15e-41 162
MS.gene82233.t1 AT1G67530 26.912 680 375 23 90 666 46 706 1.15e-41 162
MS.gene82233.t1 AT1G67530 26.912 680 375 23 90 666 46 706 1.15e-41 162
MS.gene82233.t1 AT1G27910 24.554 672 398 20 90 666 46 703 7.90e-37 148
MS.gene82233.t1 AT4G21350 32.308 390 231 10 282 665 5 367 1.93e-32 129
MS.gene82233.t1 AT1G67530 27.579 475 234 18 90 469 46 505 6.57e-30 125
MS.gene82233.t1 AT1G49780 29.412 408 253 12 277 666 9 399 7.93e-28 117
MS.gene82233.t1 AT5G18320 28.358 402 255 12 282 671 72 452 1.37e-27 117
MS.gene82233.t1 AT3G19380 29.282 362 222 12 278 622 10 354 2.67e-27 115
MS.gene82233.t1 AT1G24330 29.773 309 166 10 90 354 46 347 3.68e-26 115
MS.gene82233.t1 AT1G24330 29.773 309 166 10 90 354 46 347 3.68e-26 115
MS.gene82233.t1 AT5G67340 32.872 289 170 10 385 666 426 697 7.29e-25 110
MS.gene82233.t1 AT5G67340 53.425 73 34 0 282 354 240 312 1.90e-20 96.7
MS.gene82233.t1 AT5G67340 32.872 289 170 10 385 666 426 697 7.29e-25 110
MS.gene82233.t1 AT5G67340 53.425 73 34 0 282 354 240 312 1.90e-20 96.7
MS.gene82233.t1 AT5G37490 25.182 413 254 14 279 665 28 411 1.80e-24 107
MS.gene82233.t1 AT1G66160 25.629 437 262 16 275 686 26 424 3.18e-24 106
MS.gene82233.t1 AT2G23140 32.862 283 174 8 397 674 556 827 6.52e-23 104
MS.gene82233.t1 AT2G23140 30.859 256 145 7 119 366 86 317 1.25e-22 103
MS.gene82233.t1 AT2G23140 32.862 283 174 8 397 674 553 824 6.61e-23 104
MS.gene82233.t1 AT2G23140 30.859 256 145 7 119 366 83 314 1.27e-22 103
MS.gene82233.t1 AT2G23140 32.862 283 174 8 397 674 553 824 6.61e-23 104
MS.gene82233.t1 AT2G23140 30.859 256 145 7 119 366 83 314 1.27e-22 103
MS.gene82233.t1 AT2G23140 33.216 283 173 9 397 674 364 635 1.64e-22 103
MS.gene82233.t1 AT2G23140 42.105 95 55 0 272 366 31 125 1.09e-21 100
MS.gene82233.t1 AT2G23140 33.216 283 173 9 397 674 364 635 1.64e-22 103
MS.gene82233.t1 AT2G23140 42.105 95 55 0 272 366 31 125 1.09e-21 100
MS.gene82233.t1 AT3G54790 54.795 73 33 0 282 354 202 274 6.66e-22 101
MS.gene82233.t1 AT3G54790 30.515 272 158 7 408 666 459 712 2.31e-18 90.1
MS.gene82233.t1 AT3G54790 54.795 73 33 0 282 354 238 310 9.93e-22 100
MS.gene82233.t1 AT3G54790 30.515 272 158 7 408 666 495 748 1.74e-18 90.5
MS.gene82233.t1 AT3G54790 54.795 73 33 0 282 354 238 310 9.93e-22 100
MS.gene82233.t1 AT3G54790 30.515 272 158 7 408 666 495 748 1.74e-18 90.5
MS.gene82233.t1 AT3G49810 26.611 357 223 10 282 628 64 391 6.06e-21 97.1
MS.gene82233.t1 AT5G64660 50.667 75 37 0 274 348 2 76 1.17e-20 95.5
MS.gene82233.t1 AT2G35930 32.086 187 96 5 279 458 9 171 4.07e-20 94.0
MS.gene82233.t1 AT5G65920 24.930 357 229 10 282 628 60 387 1.17e-19 92.8
MS.gene82233.t1 AT5G65920 24.930 357 229 10 282 628 60 387 1.17e-19 92.8
MS.gene82233.t1 AT3G52450 52.778 72 33 1 279 349 4 75 2.34e-19 92.0
MS.gene82233.t1 AT3G01400 29.180 305 183 8 385 675 63 348 5.29e-19 89.7
MS.gene82233.t1 AT3G18710 50.725 69 34 0 280 348 10 78 8.28e-19 90.1
MS.gene82233.t1 AT5G09800 52.239 67 32 0 282 348 11 77 1.05e-18 89.7
MS.gene82233.t1 AT5G18340 26.780 295 193 9 271 557 63 342 1.18e-18 89.7
MS.gene82233.t1 AT3G07360 28.829 333 212 12 344 667 1 317 1.49e-18 87.8
MS.gene82233.t1 AT3G07360 28.829 333 212 12 344 667 1 317 1.49e-18 87.8
MS.gene82233.t1 AT3G07360 28.829 333 212 12 344 667 1 317 1.49e-18 87.8
MS.gene82233.t1 AT3G07360 28.829 333 212 12 344 667 1 317 1.49e-18 87.8
MS.gene82233.t1 AT3G07360 28.829 333 212 12 344 667 1 317 1.49e-18 87.8
MS.gene82233.t1 AT3G07360 28.829 333 212 12 344 667 1 317 1.49e-18 87.8
MS.gene82233.t1 AT3G07360 28.829 333 212 12 344 667 1 317 1.49e-18 87.8
MS.gene82233.t1 AT3G07360 28.829 333 212 12 344 667 1 317 1.49e-18 87.8
MS.gene82233.t1 AT3G07360 28.829 333 212 12 344 667 1 317 1.49e-18 87.8
MS.gene82233.t1 AT5G58680 29.496 278 182 7 395 667 71 339 4.82e-17 84.0
MS.gene82233.t1 AT1G01660 51.515 66 32 0 283 348 498 563 2.67e-16 83.2
MS.gene82233.t1 AT1G01660 51.515 66 32 0 283 348 499 564 2.72e-16 83.2
MS.gene82233.t1 AT1G66160 23.799 437 254 17 275 686 26 408 1.95e-15 79.7
MS.gene82233.t1 AT2G45910 40.449 89 53 0 264 352 745 833 5.55e-15 79.3
MS.gene82233.t1 AT2G45910 40.449 89 53 0 264 352 745 833 5.55e-15 79.3
MS.gene82233.t1 AT1G01680 44.737 76 42 0 273 348 224 299 9.23e-15 76.3
MS.gene82233.t1 AT2G45920 48.485 66 34 0 283 348 326 391 1.72e-14 76.6
MS.gene82233.t1 AT3G11840 44.737 76 40 2 279 353 7 81 1.90e-13 73.6
MS.gene82233.t1 AT1G08315 26.888 331 188 14 377 677 4 310 7.86e-13 70.5
MS.gene82233.t1 AT1G56040 46.032 63 34 0 283 345 354 416 8.43e-13 71.2
MS.gene82233.t1 AT3G49060 41.892 74 43 0 275 348 695 768 1.03e-12 72.0
MS.gene82233.t1 AT3G49060 41.892 74 43 0 275 348 728 801 1.05e-12 72.0
MS.gene82233.t1 AT3G49060 41.892 74 43 0 275 348 718 791 1.12e-12 72.0
MS.gene82233.t1 AT4G16490 30.824 279 150 10 408 666 206 461 1.43e-12 70.9
MS.gene82233.t1 AT5G57035 43.939 66 37 0 283 348 709 774 2.03e-12 71.2
MS.gene82233.t1 AT3G61390 43.939 66 37 0 283 348 279 344 3.54e-12 68.9
MS.gene82233.t1 AT3G61390 43.939 66 37 0 283 348 354 419 5.75e-12 68.9
MS.gene82233.t1 AT5G40140 26.027 292 180 11 390 666 236 506 9.43e-12 68.6
MS.gene82233.t1 AT2G19410 44.928 69 37 1 280 348 709 776 1.95e-11 68.2
MS.gene82233.t1 AT2G19410 44.928 69 37 1 280 348 723 790 2.11e-11 67.8
MS.gene82233.t1 AT2G19410 44.928 69 37 1 280 348 744 811 2.13e-11 67.8
MS.gene82233.t1 AT5G62560 27.055 292 186 8 385 666 239 513 6.45e-11 65.9

Find 174 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTTAAAGTTAGAGATATT+TGG 0.172217 4.2:-75070181 None:intergenic
TGTTTATGAGCTTCGGGTTT+TGG 0.199756 4.2:+75071326 MS.gene82233:CDS
GGTTAAAGTTAGAGATATTT+GGG 0.210849 4.2:-75070180 None:intergenic
ACACCATGGAGCTGCGTTTA+AGG 0.211733 4.2:-75070242 None:intergenic
AGCTGGAGCTATAGAGATTT+TGG 0.212053 4.2:+75071386 MS.gene82233:CDS
ATTAAGCTTTGGATGGATTC+TGG 0.220926 4.2:+75071045 MS.gene82233:CDS
TTCAAGTTCATTTCTTGAAT+TGG 0.244410 4.2:-75070556 None:intergenic
CTGCGTTTAAGGAACATATT+TGG 0.265739 4.2:-75070231 None:intergenic
GATAATTTCAACAAATTAAC+AGG 0.267688 4.2:+75070505 MS.gene82233:CDS
CATACCACCGTTAACTTTAA+CGG 0.270519 4.2:-75071196 None:intergenic
AAGGGTGATTTGGGAGTTAA+TGG 0.283097 4.2:+75072031 MS.gene82233:CDS
TGAGAAATATGATAGCTATT+TGG 0.284416 4.2:+75071133 MS.gene82233:CDS
GCTACTGTGTTTAGTTTAAC+TGG 0.293033 4.2:+75071597 MS.gene82233:CDS
CTGATATTGAATTACGAATT+AGG 0.306908 4.2:-75070336 None:intergenic
ACGTCGGTGGGCGGCGGGTT+TGG 0.311205 4.2:+75072106 MS.gene82233:CDS
TGGTGGTTCCGGCGGATTAC+CGG 0.317373 4.2:+75070950 MS.gene82233:CDS
TGCGTCCCTCTTCGCTCCCT+CGG 0.332883 4.2:-75071697 None:intergenic
CGGGAAATGATGGCCGTTTA+CGG 0.340552 4.2:+75070908 MS.gene82233:CDS
GGCGGTTGCGGCAGCGTTTG+TGG 0.341028 4.2:+75071872 MS.gene82233:CDS
GGAGATGACAGCGGGGATTA+TGG 0.342183 4.2:+75071788 MS.gene82233:CDS
GCGGTTGCGGCAGCGTTTGT+GGG 0.350026 4.2:+75071873 MS.gene82233:CDS
ATGGCGTTGTTTATGAGCTT+CGG 0.353792 4.2:+75071319 MS.gene82233:CDS
CTGCTCGAAGATTGTTCTAA+TGG 0.358850 4.2:+75070442 MS.gene82233:CDS
ACGGTGTGGAAAGGGTGATT+TGG 0.359234 4.2:+75072021 MS.gene82233:CDS
AAAATTGGGGACGGTGTTAA+GGG 0.365091 4.2:+75071902 MS.gene82233:CDS
AAATGTATATTGTGCTTCAT+AGG 0.366592 4.2:+75070410 MS.gene82233:CDS
TGGCGTTGTTTATGAGCTTC+GGG 0.367648 4.2:+75071320 MS.gene82233:CDS
GTCGTTGGAATTGGTGGTTC+CGG 0.372795 4.2:+75070939 MS.gene82233:CDS
CGCATCAGTTCAAGACTTAT+CGG 0.383935 4.2:-75070976 None:intergenic
GAACAGAAAATTCCATTTGA+AGG 0.385995 4.2:+75071162 MS.gene82233:CDS
CGGTGTGGAAAGGGTGATTT+GGG 0.387333 4.2:+75072022 MS.gene82233:CDS
AGCGAATAAGACAAGGATAA+TGG 0.391442 4.2:+75071497 MS.gene82233:CDS
TTAAGCTTTGGATGGATTCT+GGG 0.392841 4.2:+75071046 MS.gene82233:CDS
GAACAGAAACAACGTCGTTT+CGG 0.392901 4.2:-75070663 None:intergenic
GCGGAATCAGTCGTTGGAAT+TGG 0.393295 4.2:+75070930 MS.gene82233:CDS
ATGGCGGTGGGGAGTGTTAG+AGG 0.398283 4.2:+75072050 MS.gene82233:CDS
GATCCTGTCGTCGTGGCAAC+AGG 0.400951 4.2:+75071000 MS.gene82233:CDS
TCAGAAACGAAGAGTTTGTT+AGG 0.401404 4.2:+75070622 MS.gene82233:CDS
GATTTGGGAGTTAATGGCGG+TGG 0.402348 4.2:+75072037 MS.gene82233:CDS
GTATTAACTCCGTGTGGGCC+AGG 0.406263 4.2:-75071098 None:intergenic
ATCGTGTTTCGATTAAGCTT+TGG 0.414902 4.2:+75071034 MS.gene82233:CDS
TGGGCGGCGGGTTTGGATGG+TGG 0.427448 4.2:+75072113 MS.gene82233:CDS
GGCGGTAATGCCGGAGGAGG+CGG 0.437841 4.2:+75071809 MS.gene82233:CDS
GATGATGGTGGTTTAGTAGT+TGG 0.439712 4.2:-75070136 None:intergenic
ACGACTGATTCCGCCGTAAA+CGG 0.442880 4.2:-75070921 None:intergenic
GCGGAGCTTGCGGCAATTCA+CGG 0.444200 4.2:+75072002 MS.gene82233:CDS
GACGTTGTTTCTGTTCTAGA+AGG 0.453096 4.2:+75070670 MS.gene82233:CDS
GGGAGGGATCAGAGAGGGCG+AGG 0.456168 4.2:+75071922 MS.gene82233:CDS
CACAACACGTGTCCTAAAAC+AGG 0.458381 4.2:+75071069 MS.gene82233:CDS
GGGGATTATGGCGGTAATGC+CGG 0.458721 4.2:+75071800 MS.gene82233:CDS
CGGTGGGGAGTGTTAGAGGG+AGG 0.459782 4.2:+75072054 MS.gene82233:CDS
GAACTTGAATGATGATGTAT+TGG 0.460758 4.2:+75070570 MS.gene82233:CDS
AGCGAAGAGGGACGCATTGG+CGG 0.461449 4.2:+75071704 MS.gene82233:CDS
TATGACAAATAAGACAGCTT+TGG 0.462456 4.2:+75071215 MS.gene82233:CDS
GGGGCCGTGGAGATGACAGC+GGG 0.467255 4.2:+75071780 MS.gene82233:CDS
ATTTGGGAGTTAATGGCGGT+GGG 0.475030 4.2:+75072038 MS.gene82233:CDS
ATATTTCTCAAAGCATGGTT+AGG 0.475353 4.2:-75071120 None:intergenic
GGTTAAGCGTGGAGGATTAG+TGG 0.478476 4.2:+75071851 MS.gene82233:CDS
TTCAAGAAGAAATTCAGAAT+CGG 0.481818 4.2:+75070794 MS.gene82233:CDS
CGAGCTTGTATAGCTGAAGC+TGG 0.483143 4.2:+75071369 MS.gene82233:CDS
GCGTCCCTCTTCGCTCCCTC+GGG 0.484522 4.2:-75071696 None:intergenic
AACGACGCCGTTTCTGATGA+TGG 0.489185 4.2:-75070151 None:intergenic
GGCAATTCACGGTGTGGAAA+GGG 0.492451 4.2:+75072013 MS.gene82233:CDS
TGTCCTGTTGCCACGACGAC+AGG 0.493205 4.2:-75071003 None:intergenic
AGTATCGTCACCGCCTCCTC+CGG 0.493654 4.2:-75071819 None:intergenic
TCAGAGATTGTTGCGTTGAT+CGG 0.493804 4.2:+75070835 MS.gene82233:CDS
CGGCAATTCACGGTGTGGAA+AGG 0.494115 4.2:+75072012 MS.gene82233:CDS
GAGTGTTAGAGGGAGGAGGA+AGG 0.494415 4.2:+75072061 MS.gene82233:CDS
TTTGCATCTCGCAGCGGATA+GGG 0.494746 4.2:+75071731 MS.gene82233:CDS
CGTTAGTGGATTGGTTGAGT+TGG 0.496851 4.2:+75071662 MS.gene82233:CDS
TGTGCTAATGAAGTTGTAGT+TGG 0.496874 4.2:-75072197 None:intergenic
GTTGGTAGAGAGTGGGGCCG+TGG 0.498825 4.2:+75071767 MS.gene82233:CDS
TTCGCAAAATCCAAGCCTAC+AGG 0.499199 4.2:+75071434 MS.gene82233:CDS
GGGTTTGGATGGTGGTGAAA+CGG 0.499778 4.2:+75072121 MS.gene82233:CDS
TATTAACTCCGTGTGGGCCA+GGG 0.501113 4.2:-75071097 None:intergenic
GCTACCGTTTCACCTTCAAA+TGG 0.501489 4.2:-75071174 None:intergenic
GGTGCAACGACGGCGTCGAC+GGG 0.502990 4.2:+75070889 MS.gene82233:CDS
GACACGAATCGTTAGTGGAT+TGG 0.503002 4.2:+75071653 MS.gene82233:CDS
ACTGCATTAACCTGTAGGCT+TGG 0.503198 4.2:-75071444 None:intergenic
CGGTGGGCGGCGGGTTTGGA+TGG 0.505339 4.2:+75072110 MS.gene82233:CDS
TAACGGTTCTTTAGCGTCGA+TGG 0.506187 4.2:+75071287 MS.gene82233:CDS
TGTGTTTAGTTTAACTGGAG+TGG 0.506633 4.2:+75071602 MS.gene82233:CDS
TGGAGGATTAGTGGCGGTTG+CGG 0.506660 4.2:+75071860 MS.gene82233:CDS
AATTCCATTTGAAGGTGAAA+CGG 0.509042 4.2:+75071170 MS.gene82233:CDS
ACGGTAGCCGTTAAAGTTAA+CGG 0.510244 4.2:+75071189 MS.gene82233:CDS
TGGTGCAACGACGGCGTCGA+CGG 0.513053 4.2:+75070888 MS.gene82233:CDS
AGGAAGACACGAATCGTTAG+TGG 0.516476 4.2:+75071648 MS.gene82233:CDS
TGGACCCGAGGGAGCGAAGA+GGG 0.520213 4.2:+75071692 MS.gene82233:CDS
GAATCAGTCGTTGGAATTGG+TGG 0.520402 4.2:+75070933 MS.gene82233:CDS
TAGGGAGACGGTGGGAAGGT+TGG 0.520508 4.2:+75071749 MS.gene82233:CDS
TGGGGAGTGTTAGAGGGAGG+AGG 0.524460 4.2:+75072057 MS.gene82233:CDS
GGAGGGATCAGAGAGGGCGA+GGG 0.524599 4.2:+75071923 MS.gene82233:CDS
ACGGCGTCGACGGGAAATGA+TGG 0.528688 4.2:+75070898 MS.gene82233:CDS
TATGGCGGTAATGCCGGAGG+AGG 0.529298 4.2:+75071806 MS.gene82233:CDS
TTGTGAGGTGTAATAGAGAA+TGG 0.534465 4.2:-75070207 None:intergenic
ATTGGGGACGGTGTTAAGGG+AGG 0.534820 4.2:+75071905 MS.gene82233:CDS
ATACGTAAAACGCACCTCCT+CGG 0.542514 4.2:+75070298 MS.gene82233:CDS
GATAAGTCTTGAACTGATGC+GGG 0.544369 4.2:+75070978 MS.gene82233:CDS
GGGAGCGAAGAGGGACGCAT+TGG 0.545015 4.2:+75071701 MS.gene82233:CDS
TGTTTCGATTAAGCTTTGGA+TGG 0.545411 4.2:+75071038 MS.gene82233:CDS
AGAGATTGTGGCGGAGCTTG+CGG 0.545486 4.2:+75071992 MS.gene82233:CDS
TTGGATGGTGGTGAAACGGA+GGG 0.546989 4.2:+75072125 MS.gene82233:CDS
TGGGAAGGTTGGTAGAGAGT+GGG 0.549322 4.2:+75071760 MS.gene82233:CDS
GGTGTAATAGAGAATGGAGG+AGG 0.550947 4.2:-75070201 None:intergenic
GGAAGGCAGCGACGTTGTTG+AGG 0.556452 4.2:+75072078 MS.gene82233:CDS
CGATAAGTCTTGAACTGATG+CGG 0.556842 4.2:+75070977 MS.gene82233:CDS
TAAACCACCATCATCAGAAA+CGG 0.559799 4.2:+75070144 MS.gene82233:CDS
GTGGGAAGGTTGGTAGAGAG+TGG 0.562347 4.2:+75071759 MS.gene82233:CDS
AGGAACATATTTGGTTTGTG+AGG 0.564288 4.2:-75070222 None:intergenic
CTATCATATTTCTCAAAGCA+TGG 0.564762 4.2:-75071125 None:intergenic
ATCGGACACCGGTAATCCGC+CGG 0.567196 4.2:-75070958 None:intergenic
GCGTCGATGGAAGAGACAAA+TGG 0.569992 4.2:+75071300 MS.gene82233:CDS
AGAGAGGGCGAGGGAGAGCG+CGG 0.576119 4.2:+75071932 MS.gene82233:CDS
TTAGTAGTTGGAAATGTTTG+AGG 0.577855 4.2:-75070124 None:intergenic
GTTCCTTAAACGCAGCTCCA+TGG 0.581251 4.2:+75070239 MS.gene82233:CDS
GGGAAGGTTGGTAGAGAGTG+GGG 0.582589 4.2:+75071761 MS.gene82233:CDS
GGTGATTTGGGAGTTAATGG+CGG 0.585091 4.2:+75072034 MS.gene82233:CDS
CGGATAGGGAGACGGTGGGA+AGG 0.585973 4.2:+75071745 MS.gene82233:CDS
CGCAGCGGATAGGGAGACGG+TGG 0.589169 4.2:+75071740 MS.gene82233:CDS
GTTGGAATTGGTGGTTCCGG+CGG 0.589268 4.2:+75070942 MS.gene82233:CDS
TTGTAACTGCATTAACCTGT+AGG 0.590589 4.2:-75071449 None:intergenic
TCCTAAAACAGGTCAAACCC+TGG 0.592266 4.2:+75071080 MS.gene82233:CDS
AGGTCAAACCCTGGCCCACA+CGG 0.592317 4.2:+75071089 MS.gene82233:CDS
CGGTTGCGGCAGCGTTTGTG+GGG 0.593066 4.2:+75071874 MS.gene82233:CDS
TGAGGTGTAATAGAGAATGG+AGG 0.594765 4.2:-75070204 None:intergenic
TTTGGATGGTGGTGAAACGG+AGG 0.595577 4.2:+75072124 MS.gene82233:CDS
TTTACGGCGGAATCAGTCGT+TGG 0.597846 4.2:+75070924 MS.gene82233:CDS
GGTTAGGTATTAACTCCGTG+TGG 0.601915 4.2:-75071104 None:intergenic
TGGCGGTGGGGAGTGTTAGA+GGG 0.603345 4.2:+75072051 MS.gene82233:CDS
GAAAGGTGGATCAGAGATTG+TGG 0.605941 4.2:+75071980 MS.gene82233:CDS
AATCCCCGCTGTCATCTCCA+CGG 0.607104 4.2:-75071784 None:intergenic
TGTTAGTACATCAACTACCA+TGG 0.608462 4.2:+75072169 MS.gene82233:CDS
GTTAAGGGAGGGATCAGAGA+GGG 0.610803 4.2:+75071917 MS.gene82233:CDS
TTTAAGATCGGGTGCCACGT+GGG 0.611826 4.2:+75071554 MS.gene82233:CDS
TTGGGGACGGTGTTAAGGGA+GGG 0.620299 4.2:+75071906 MS.gene82233:CDS
TTTGGAAGCAACGAGAATGA+CGG 0.620433 4.2:+75071233 MS.gene82233:CDS
AACAAGTTGAAGTTGACTAA+CGG 0.623348 4.2:+75071270 MS.gene82233:CDS
TCTCGCAGCGGATAGGGAGA+CGG 0.625914 4.2:+75071737 MS.gene82233:CDS
GATTATGGCGGTAATGCCGG+AGG 0.627766 4.2:+75071803 MS.gene82233:CDS
GTTAAAGTTAGAGATATTTG+GGG 0.630297 4.2:-75070179 None:intergenic
AAGATCGGGTGCCACGTGGG+AGG 0.630647 4.2:+75071557 MS.gene82233:CDS
CGACACTTGTGACAATGTGT+AGG 0.637316 4.2:+75071958 MS.gene82233:CDS
TTTGGGAGTTAATGGCGGTG+GGG 0.641101 4.2:+75072039 MS.gene82233:CDS
CTTGAAGCAGTGGTTAAGCG+TGG 0.642024 4.2:+75071840 MS.gene82233:CDS
GGTGACGATACTTGAAGCAG+TGG 0.647555 4.2:+75071830 MS.gene82233:CDS
CTTGTGACAATGTGTAGGAA+AGG 0.647925 4.2:+75071963 MS.gene82233:CDS
GTTAGGTATTAACTCCGTGT+GGG 0.650428 4.2:-75071103 None:intergenic
ACAAGGATAATGGAGACTGA+AGG 0.650963 4.2:+75071507 MS.gene82233:CDS
TGGGGCCGTGGAGATGACAG+CGG 0.650969 4.2:+75071779 MS.gene82233:CDS
GCAGCGGATAGGGAGACGGT+GGG 0.651330 4.2:+75071741 MS.gene82233:CDS
ATGGACCCGAGGGAGCGAAG+AGG 0.654485 4.2:+75071691 MS.gene82233:CDS
GTGACAATGTGTAGGAAAGG+TGG 0.655053 4.2:+75071966 MS.gene82233:CDS
TTTAAAAGGAAACGACACCA+TGG 0.659696 4.2:-75070256 None:intergenic
TACTTGAAGCGAATAAGACA+AGG 0.661909 4.2:+75071490 MS.gene82233:CDS
GTGGGAGGCTAAAGCAAATG+CGG 0.672161 4.2:+75071572 MS.gene82233:CDS
GACGCCGTTTCTGATGATGG+TGG 0.677070 4.2:-75070148 None:intergenic
GCTTGCGGCAATTCACGGTG+TGG 0.678917 4.2:+75072007 MS.gene82233:CDS
GATGCGGGATCCTGTCGTCG+TGG 0.685189 4.2:+75070993 MS.gene82233:CDS
TAAGCGTGGAGGATTAGTGG+CGG 0.685593 4.2:+75071854 MS.gene82233:CDS
GGAGGCTAAAGCAAATGCGG+CGG 0.686035 4.2:+75071575 MS.gene82233:CDS
GATGACAGCGGGGATTATGG+CGG 0.687573 4.2:+75071791 MS.gene82233:CDS
GAAATGATGGCCGTTTACGG+CGG 0.687834 4.2:+75070911 MS.gene82233:CDS
GTTCAAGACTTATCGGACAC+CGG 0.688916 4.2:-75070969 None:intergenic
ATTTGCTTTAGCCTCCCACG+TGG 0.692219 4.2:-75071568 None:intergenic
AGTTGTAGTTGGAACAACCA+TGG 0.693372 4.2:-75072186 None:intergenic
GAAGCAGTGGTTAAGCGTGG+AGG 0.694054 4.2:+75071843 MS.gene82233:CDS
ACACATTGTCACAAGTGTCG+CGG 0.696733 4.2:-75071955 None:intergenic
CATCGAAGACGACACCGAGG+AGG 0.702271 4.2:-75070312 None:intergenic
AGGTGGATCAGAGATTGTGG+CGG 0.710219 4.2:+75071983 MS.gene82233:CDS
GGAAGCAACGAGAATGACGG+CGG 0.724049 4.2:+75071236 MS.gene82233:CDS
TGTTAAGGGAGGGATCAGAG+AGG 0.725744 4.2:+75071916 MS.gene82233:CDS
GGGCCGTGGAGATGACAGCG+GGG 0.731568 4.2:+75071781 MS.gene82233:CDS
GGTCATCGAAGACGACACCG+AGG 0.738985 4.2:-75070315 None:intergenic
GTAGCCGTTAAAGTTAACGG+TGG 0.777768 4.2:+75071192 MS.gene82233:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAATTTCAACAAATTAAC+AGG + chr4.2:75070505-75070524 MS.gene82233:CDS 20.0%
!!! AAGAGGAAATTTTGTTTAAA+AGG - chr4.2:75070273-75070292 None:intergenic 20.0%
!!! ATCTTCAATTTTTGAGAAAT+TGG + chr4.2:75070729-75070748 MS.gene82233:CDS 20.0%
!!! GAAGATAATTTTTCATCTTT+TGG - chr4.2:75070715-75070734 None:intergenic 20.0%
! AAATGTATATTGTGCTTCAT+AGG + chr4.2:75070410-75070429 MS.gene82233:CDS 25.0%
! AGGTTAAAGTTAGAGATATT+TGG - chr4.2:75070184-75070203 None:intergenic 25.0%
! CTGATATTGAATTACGAATT+AGG - chr4.2:75070339-75070358 None:intergenic 25.0%
! GGTTAAAGTTAGAGATATTT+GGG - chr4.2:75070183-75070202 None:intergenic 25.0%
! GTTAAAGTTAGAGATATTTG+GGG - chr4.2:75070182-75070201 None:intergenic 25.0%
! TGAGAAATATGATAGCTATT+TGG + chr4.2:75071133-75071152 MS.gene82233:CDS 25.0%
! TTCAAGAAGAAATTCAGAAT+CGG + chr4.2:75070794-75070813 MS.gene82233:CDS 25.0%
! TTTGTGGGGATAAAAAAATT+GGG + chr4.2:75071888-75071907 MS.gene82233:CDS 25.0%
!! TTCAAGTTCATTTCTTGAAT+TGG - chr4.2:75070559-75070578 None:intergenic 25.0%
AATTCCATTTGAAGGTGAAA+CGG + chr4.2:75071170-75071189 MS.gene82233:CDS 30.0%
ATATTTCTCAAAGCATGGTT+AGG - chr4.2:75071123-75071142 None:intergenic 30.0%
CTATCATATTTCTCAAAGCA+TGG - chr4.2:75071128-75071147 None:intergenic 30.0%
GAACAGAAAATTCCATTTGA+AGG + chr4.2:75071162-75071181 MS.gene82233:CDS 30.0%
GTTTGTGGGGATAAAAAAAT+TGG + chr4.2:75071887-75071906 MS.gene82233:CDS 30.0%
TATGACAAATAAGACAGCTT+TGG + chr4.2:75071215-75071234 MS.gene82233:CDS 30.0%
TTAGTAGTTGGAAATGTTTG+AGG - chr4.2:75070127-75070146 None:intergenic 30.0%
TTGTGGGGATAAAAAAATTG+GGG + chr4.2:75071889-75071908 MS.gene82233:CDS 30.0%
! AACAAGTTGAAGTTGACTAA+CGG + chr4.2:75071270-75071289 MS.gene82233:CDS 30.0%
! GAACTTGAATGATGATGTAT+TGG + chr4.2:75070570-75070589 MS.gene82233:CDS 30.0%
AGCGAATAAGACAAGGATAA+TGG + chr4.2:75071497-75071516 MS.gene82233:CDS 35.0%
ATCGTGTTTCGATTAAGCTT+TGG + chr4.2:75071034-75071053 MS.gene82233:CDS 35.0%
CATACCACCGTTAACTTTAA+CGG - chr4.2:75071199-75071218 None:intergenic 35.0%
CTGCGTTTAAGGAACATATT+TGG - chr4.2:75070234-75070253 None:intergenic 35.0%
TAAACCACCATCATCAGAAA+CGG + chr4.2:75070144-75070163 MS.gene82233:CDS 35.0%
TACTTGAAGCGAATAAGACA+AGG + chr4.2:75071490-75071509 MS.gene82233:CDS 35.0%
TGTGCTAATGAAGTTGTAGT+TGG - chr4.2:75072200-75072219 None:intergenic 35.0%
TGTTAGTACATCAACTACCA+TGG + chr4.2:75072169-75072188 MS.gene82233:CDS 35.0%
TTGTAACTGCATTAACCTGT+AGG - chr4.2:75071452-75071471 None:intergenic 35.0%
TTGTGAGGTGTAATAGAGAA+TGG - chr4.2:75070210-75070229 None:intergenic 35.0%
TTTAAAAGGAAACGACACCA+TGG - chr4.2:75070259-75070278 None:intergenic 35.0%
! GCTACTGTGTTTAGTTTAAC+TGG + chr4.2:75071597-75071616 MS.gene82233:CDS 35.0%
! TCAGAAACGAAGAGTTTGTT+AGG + chr4.2:75070622-75070641 MS.gene82233:CDS 35.0%
! TGTGTTTAGTTTAACTGGAG+TGG + chr4.2:75071602-75071621 MS.gene82233:CDS 35.0%
!! AGGAACATATTTGGTTTGTG+AGG - chr4.2:75070225-75070244 None:intergenic 35.0%
!! ATTAAGCTTTGGATGGATTC+TGG + chr4.2:75071045-75071064 MS.gene82233:CDS 35.0%
!! TGTTTCGATTAAGCTTTGGA+TGG + chr4.2:75071038-75071057 MS.gene82233:CDS 35.0%
!! TTAAGCTTTGGATGGATTCT+GGG + chr4.2:75071046-75071065 MS.gene82233:CDS 35.0%
!!! CGGTTTTTAAGTTACGAAGT+TGG + chr4.2:75071411-75071430 MS.gene82233:CDS 35.0%
!!! GAGCTATAGAGATTTTGGTT+CGG + chr4.2:75071391-75071410 MS.gene82233:CDS 35.0%
ACGGTAGCCGTTAAAGTTAA+CGG + chr4.2:75071189-75071208 MS.gene82233:CDS 40.0%
AGTTGTAGTTGGAACAACCA+TGG - chr4.2:75072189-75072208 None:intergenic 40.0%
ATGGCGTTGTTTATGAGCTT+CGG + chr4.2:75071319-75071338 MS.gene82233:CDS 40.0%
CGATAAGTCTTGAACTGATG+CGG + chr4.2:75070977-75070996 MS.gene82233:CDS 40.0%
CGCATCAGTTCAAGACTTAT+CGG - chr4.2:75070979-75070998 None:intergenic 40.0%
CTGCTCGAAGATTGTTCTAA+TGG + chr4.2:75070442-75070461 MS.gene82233:CDS 40.0%
CTTGTGACAATGTGTAGGAA+AGG + chr4.2:75071963-75071982 MS.gene82233:CDS 40.0%
GAACAGAAACAACGTCGTTT+CGG - chr4.2:75070666-75070685 None:intergenic 40.0%
GATAAGTCTTGAACTGATGC+GGG + chr4.2:75070978-75070997 MS.gene82233:CDS 40.0%
GGGGATAAAAAAATTGGGGA+CGG + chr4.2:75071893-75071912 MS.gene82233:CDS 40.0%
GTTAGGTATTAACTCCGTGT+GGG - chr4.2:75071106-75071125 None:intergenic 40.0%
TGAGGTGTAATAGAGAATGG+AGG - chr4.2:75070207-75070226 None:intergenic 40.0%
TTTGGAAGCAACGAGAATGA+CGG + chr4.2:75071233-75071252 MS.gene82233:CDS 40.0%
! AAAAATTGGGGACGGTGTTA+AGG + chr4.2:75071901-75071920 MS.gene82233:CDS 40.0%
! AAAATTGGGGACGGTGTTAA+GGG + chr4.2:75071902-75071921 MS.gene82233:CDS 40.0%
! AAGGGTGATTTGGGAGTTAA+TGG + chr4.2:75072031-75072050 MS.gene82233:CDS 40.0%
! AGCTGGAGCTATAGAGATTT+TGG + chr4.2:75071386-75071405 MS.gene82233:CDS 40.0%
! CGTTGTTGAGGATTTTACGT+CGG + chr4.2:75072090-75072109 MS.gene82233:CDS 40.0%
! TATGCGAAGTGCGTTCTTTT+TGG + chr4.2:75070868-75070887 MS.gene82233:CDS 40.0%
! TCAGAGATTGTTGCGTTGAT+CGG + chr4.2:75070835-75070854 MS.gene82233:CDS 40.0%
!! ACAAGGATAATGGAGACTGA+AGG + chr4.2:75071507-75071526 MS.gene82233:CDS 40.0%
!! CGTTTTACGTATAGTGGAAG+AGG - chr4.2:75070290-75070309 None:intergenic 40.0%
!! CTTTTAGAAAGCGGTTAGGT+AGG + chr4.2:75071628-75071647 MS.gene82233:CDS 40.0%
!! GACGTTGTTTCTGTTCTAGA+AGG + chr4.2:75070670-75070689 MS.gene82233:CDS 40.0%
!! GATGATGGTGGTTTAGTAGT+TGG - chr4.2:75070139-75070158 None:intergenic 40.0%
!! TGTTTATGAGCTTCGGGTTT+TGG + chr4.2:75071326-75071345 MS.gene82233:CDS 40.0%
!! TTGGTTGAGTTGGCGAAAAA+TGG + chr4.2:75071672-75071691 MS.gene82233:CDS 40.0%
!!! GAGACTGAAGGTGTTTTAAG+TGG + chr4.2:75071519-75071538 MS.gene82233:CDS 40.0%
!!! GAGTGGTTGCTTTTAGAAAG+CGG + chr4.2:75071619-75071638 MS.gene82233:CDS 40.0%
!!! GTTGCTTTTAGAAAGCGGTT+AGG + chr4.2:75071624-75071643 MS.gene82233:CDS 40.0%
!!! TGAAGGTGTTTTAAGTGGTG+TGG + chr4.2:75071524-75071543 MS.gene82233:CDS 40.0%
!!! TGTGGCAGAGGTTTTAAGAT+CGG + chr4.2:75071542-75071561 MS.gene82233:CDS 40.0%
ACTGCATTAACCTGTAGGCT+TGG - chr4.2:75071447-75071466 None:intergenic 45.0%
AGGAAGACACGAATCGTTAG+TGG + chr4.2:75071648-75071667 MS.gene82233:CDS 45.0%
ATACGTAAAACGCACCTCCT+CGG + chr4.2:75070298-75070317 MS.gene82233:CDS 45.0%
ATTTGGGAGTTAATGGCGGT+GGG + chr4.2:75072038-75072057 MS.gene82233:CDS 45.0%
CACAACACGTGTCCTAAAAC+AGG + chr4.2:75071069-75071088 MS.gene82233:CDS 45.0%
CGACACTTGTGACAATGTGT+AGG + chr4.2:75071958-75071977 MS.gene82233:CDS 45.0%
GAAAGGTGGATCAGAGATTG+TGG + chr4.2:75071980-75071999 MS.gene82233:CDS 45.0%
GAATCAGTCGTTGGAATTGG+TGG + chr4.2:75070933-75070952 MS.gene82233:CDS 45.0%
GCTACCGTTTCACCTTCAAA+TGG - chr4.2:75071177-75071196 None:intergenic 45.0%
GGTGTAATAGAGAATGGAGG+AGG - chr4.2:75070204-75070223 None:intergenic 45.0%
GGTTAGGTATTAACTCCGTG+TGG - chr4.2:75071107-75071126 None:intergenic 45.0%
GTAGCCGTTAAAGTTAACGG+TGG + chr4.2:75071192-75071211 MS.gene82233:CDS 45.0%
GTGACAATGTGTAGGAAAGG+TGG + chr4.2:75071966-75071985 MS.gene82233:CDS 45.0%
GTTCAAGACTTATCGGACAC+CGG - chr4.2:75070972-75070991 None:intergenic 45.0%
TCCTAAAACAGGTCAAACCC+TGG + chr4.2:75071080-75071099 MS.gene82233:CDS 45.0%
TGGCGTTGTTTATGAGCTTC+GGG + chr4.2:75071320-75071339 MS.gene82233:CDS 45.0%
TTCGCAAAATCCAAGCCTAC+AGG + chr4.2:75071434-75071453 MS.gene82233:CDS 45.0%
! ACACATTGTCACAAGTGTCG+CGG - chr4.2:75071958-75071977 None:intergenic 45.0%
! GACACGAATCGTTAGTGGAT+TGG + chr4.2:75071653-75071672 MS.gene82233:CDS 45.0%
! GAGGTGCGTTTTACGTATAG+TGG - chr4.2:75070296-75070315 None:intergenic 45.0%
! GGTGATTTGGGAGTTAATGG+CGG + chr4.2:75072034-75072053 MS.gene82233:CDS 45.0%
! GTTGAGGATTTTACGTCGGT+GGG + chr4.2:75072094-75072113 MS.gene82233:CDS 45.0%
! TAACGGTTCTTTAGCGTCGA+TGG + chr4.2:75071287-75071306 MS.gene82233:CDS 45.0%
! TGTTGAGGATTTTACGTCGG+TGG + chr4.2:75072093-75072112 MS.gene82233:CDS 45.0%
!! CGTTAGTGGATTGGTTGAGT+TGG + chr4.2:75071662-75071681 MS.gene82233:CDS 45.0%
!! CGTTCTTTTTGGTGCAACGA+CGG + chr4.2:75070879-75070898 MS.gene82233:CDS 45.0%
!! TGTTTTAAGTGGTGTGGCAG+AGG + chr4.2:75071530-75071549 MS.gene82233:CDS 45.0%
!!! GTGGCAGAGGTTTTAAGATC+GGG + chr4.2:75071543-75071562 MS.gene82233:CDS 45.0%
AACGACGCCGTTTCTGATGA+TGG - chr4.2:75070154-75070173 None:intergenic 50.0%
ACACCATGGAGCTGCGTTTA+AGG - chr4.2:75070245-75070264 None:intergenic 50.0%
ACGGTGTGGAAAGGGTGATT+TGG + chr4.2:75072021-75072040 MS.gene82233:CDS 50.0%
AGGTGGATCAGAGATTGTGG+CGG + chr4.2:75071983-75072002 MS.gene82233:CDS 50.0%
ATTTGCTTTAGCCTCCCACG+TGG - chr4.2:75071571-75071590 None:intergenic 50.0%
CGAGCTTGTATAGCTGAAGC+TGG + chr4.2:75071369-75071388 MS.gene82233:CDS 50.0%
CGGGAAATGATGGCCGTTTA+CGG + chr4.2:75070908-75070927 MS.gene82233:CDS 50.0%
CGGTGTGGAAAGGGTGATTT+GGG + chr4.2:75072022-75072041 MS.gene82233:CDS 50.0%
CTTGAAGCAGTGGTTAAGCG+TGG + chr4.2:75071840-75071859 MS.gene82233:CDS 50.0%
GAAATGATGGCCGTTTACGG+CGG + chr4.2:75070911-75070930 MS.gene82233:CDS 50.0%
GATTTGGGAGTTAATGGCGG+TGG + chr4.2:75072037-75072056 MS.gene82233:CDS 50.0%
GCGGAATCAGTCGTTGGAAT+TGG + chr4.2:75070930-75070949 MS.gene82233:CDS 50.0%
GGCAATTCACGGTGTGGAAA+GGG + chr4.2:75072013-75072032 MS.gene82233:CDS 50.0%
GGTGACGATACTTGAAGCAG+TGG + chr4.2:75071830-75071849 MS.gene82233:CDS 50.0%
GGTTAAGCGTGGAGGATTAG+TGG + chr4.2:75071851-75071870 MS.gene82233:CDS 50.0%
GTGGGAGGCTAAAGCAAATG+CGG + chr4.2:75071572-75071591 MS.gene82233:CDS 50.0%
GTTAAGGGAGGGATCAGAGA+GGG + chr4.2:75071917-75071936 MS.gene82233:CDS 50.0%
GTTCCTTAAACGCAGCTCCA+TGG + chr4.2:75070239-75070258 MS.gene82233:CDS 50.0%
TATTAACTCCGTGTGGGCCA+GGG - chr4.2:75071100-75071119 None:intergenic 50.0%
TGTTAAGGGAGGGATCAGAG+AGG + chr4.2:75071916-75071935 MS.gene82233:CDS 50.0%
TTGGCGAAAAATGGACCCGA+GGG + chr4.2:75071681-75071700 MS.gene82233:CDS 50.0%
TTTACGGCGGAATCAGTCGT+TGG + chr4.2:75070924-75070943 MS.gene82233:CDS 50.0%
TTTGCATCTCGCAGCGGATA+GGG + chr4.2:75071731-75071750 MS.gene82233:CDS 50.0%
TTTGGGAGTTAATGGCGGTG+GGG + chr4.2:75072039-75072058 MS.gene82233:CDS 50.0%
! ACGACTGATTCCGCCGTAAA+CGG - chr4.2:75070924-75070943 None:intergenic 50.0%
! GCCAGGGTTTGACCTGTTTT+AGG - chr4.2:75071084-75071103 None:intergenic 50.0%
! GCGTCGATGGAAGAGACAAA+TGG + chr4.2:75071300-75071319 MS.gene82233:CDS 50.0%
! GGCAATTTTGCATCTCGCAG+CGG + chr4.2:75071725-75071744 MS.gene82233:CDS 50.0%
! GGGTTTGGATGGTGGTGAAA+CGG + chr4.2:75072121-75072140 MS.gene82233:CDS 50.0%
! GTCGTTGGAATTGGTGGTTC+CGG + chr4.2:75070939-75070958 MS.gene82233:CDS 50.0%
! TAAGCGTGGAGGATTAGTGG+CGG + chr4.2:75071854-75071873 MS.gene82233:CDS 50.0%
! TTGGATGGTGGTGAAACGGA+GGG + chr4.2:75072125-75072144 MS.gene82233:CDS 50.0%
! TTTAAGATCGGGTGCCACGT+GGG + chr4.2:75071554-75071573 MS.gene82233:CDS 50.0%
! TTTGGATGGTGGTGAAACGG+AGG + chr4.2:75072124-75072143 MS.gene82233:CDS 50.0%
! TTTTGCATCTCGCAGCGGAT+AGG + chr4.2:75071730-75071749 MS.gene82233:CDS 50.0%
!! GCTTCGGGTTTTGGCGAAAT+CGG + chr4.2:75071335-75071354 MS.gene82233:CDS 50.0%
!! TGGGAAGGTTGGTAGAGAGT+GGG + chr4.2:75071760-75071779 MS.gene82233:CDS 50.0%
!!! TTTTAAGATCGGGTGCCACG+TGG + chr4.2:75071553-75071572 MS.gene82233:CDS 50.0%
AATCCCCGCTGTCATCTCCA+CGG - chr4.2:75071787-75071806 None:intergenic 55.0%
CGGCAATTCACGGTGTGGAA+AGG + chr4.2:75072012-75072031 MS.gene82233:CDS 55.0%
GAAGCAGTGGTTAAGCGTGG+AGG + chr4.2:75071843-75071862 MS.gene82233:CDS 55.0%
GAGTGTTAGAGGGAGGAGGA+AGG + chr4.2:75072061-75072080 MS.gene82233:CDS 55.0%
GATGACAGCGGGGATTATGG+CGG + chr4.2:75071791-75071810 MS.gene82233:CDS 55.0%
GATTATGGCGGTAATGCCGG+AGG + chr4.2:75071803-75071822 MS.gene82233:CDS 55.0%
GGAAGCAACGAGAATGACGG+CGG + chr4.2:75071236-75071255 MS.gene82233:CDS 55.0%
GGAGATGACAGCGGGGATTA+TGG + chr4.2:75071788-75071807 MS.gene82233:CDS 55.0%
GGAGGCTAAAGCAAATGCGG+CGG + chr4.2:75071575-75071594 MS.gene82233:CDS 55.0%
GGGGATTATGGCGGTAATGC+CGG + chr4.2:75071800-75071819 MS.gene82233:CDS 55.0%
GTATTAACTCCGTGTGGGCC+AGG - chr4.2:75071101-75071120 None:intergenic 55.0%
GTTGGCGAAAAATGGACCCG+AGG + chr4.2:75071680-75071699 MS.gene82233:CDS 55.0%
! AGAGATTGTGGCGGAGCTTG+CGG + chr4.2:75071992-75072011 MS.gene82233:CDS 55.0%
! ATTGGGGACGGTGTTAAGGG+AGG + chr4.2:75071905-75071924 MS.gene82233:CDS 55.0%
! GAGGATTTTACGTCGGTGGG+CGG + chr4.2:75072097-75072116 MS.gene82233:CDS 55.0%
! GTTGGAATTGGTGGTTCCGG+CGG + chr4.2:75070942-75070961 MS.gene82233:CDS 55.0%
! TGGAGGATTAGTGGCGGTTG+CGG + chr4.2:75071860-75071879 MS.gene82233:CDS 55.0%
! TTGGGGACGGTGTTAAGGGA+GGG + chr4.2:75071906-75071925 MS.gene82233:CDS 55.0%
!! GACGCCGTTTCTGATGATGG+TGG - chr4.2:75070151-75070170 None:intergenic 55.0%
!! GGGAAGGTTGGTAGAGAGTG+GGG + chr4.2:75071761-75071780 MS.gene82233:CDS 55.0%
!! GTGGGAAGGTTGGTAGAGAG+TGG + chr4.2:75071759-75071778 MS.gene82233:CDS 55.0%
AGCGAAGAGGGACGCATTGG+CGG + chr4.2:75071704-75071723 MS.gene82233:CDS 60.0%
AGGTCAAACCCTGGCCCACA+CGG + chr4.2:75071089-75071108 MS.gene82233:CDS 60.0%
AGTATCGTCACCGCCTCCTC+CGG - chr4.2:75071822-75071841 None:intergenic 60.0%
ATCGGACACCGGTAATCCGC+CGG - chr4.2:75070961-75070980 None:intergenic 60.0%
CATCGAAGACGACACCGAGG+AGG - chr4.2:75070315-75070334 None:intergenic 60.0%
GATCCTGTCGTCGTGGCAAC+AGG + chr4.2:75071000-75071019 MS.gene82233:CDS 60.0%
GCGGAGCTTGCGGCAATTCA+CGG + chr4.2:75072002-75072021 MS.gene82233:CDS 60.0%
GCTTGCGGCAATTCACGGTG+TGG + chr4.2:75072007-75072026 MS.gene82233:CDS 60.0%
GGAAGGCAGCGACGTTGTTG+AGG + chr4.2:75072078-75072097 MS.gene82233:CDS 60.0%
GGTCATCGAAGACGACACCG+AGG - chr4.2:75070318-75070337 None:intergenic 60.0%
TAGGGAGACGGTGGGAAGGT+TGG + chr4.2:75071749-75071768 MS.gene82233:CDS 60.0%
TATGGCGGTAATGCCGGAGG+AGG + chr4.2:75071806-75071825 MS.gene82233:CDS 60.0%
TCTCGCAGCGGATAGGGAGA+CGG + chr4.2:75071737-75071756 MS.gene82233:CDS 60.0%
TGTCCTGTTGCCACGACGAC+AGG - chr4.2:75071006-75071025 None:intergenic 60.0%
! ATTTTACGTCGGTGGGCGGC+GGG + chr4.2:75072101-75072120 MS.gene82233:CDS 60.0%
! GATTTTACGTCGGTGGGCGG+CGG + chr4.2:75072100-75072119 MS.gene82233:CDS 60.0%
!! ACGGCGTCGACGGGAAATGA+TGG + chr4.2:75070898-75070917 MS.gene82233:CDS 60.0%
!! ATGGCGGTGGGGAGTGTTAG+AGG + chr4.2:75072050-75072069 MS.gene82233:CDS 60.0%
!! TGGCGGTGGGGAGTGTTAGA+GGG + chr4.2:75072051-75072070 MS.gene82233:CDS 60.0%
!! TGGGGAGTGTTAGAGGGAGG+AGG + chr4.2:75072057-75072076 MS.gene82233:CDS 60.0%
!! TGGTGGTTCCGGCGGATTAC+CGG + chr4.2:75070950-75070969 MS.gene82233:CDS 60.0%
ATGGACCCGAGGGAGCGAAG+AGG + chr4.2:75071691-75071710 MS.gene82233:CDS 65.0%
CGGATAGGGAGACGGTGGGA+AGG + chr4.2:75071745-75071764 MS.gene82233:CDS 65.0%
CGGTTGCGGCAGCGTTTGTG+GGG + chr4.2:75071874-75071893 MS.gene82233:CDS 65.0%
GATGCGGGATCCTGTCGTCG+TGG + chr4.2:75070993-75071012 MS.gene82233:CDS 65.0%
GCAGCGGATAGGGAGACGGT+GGG + chr4.2:75071741-75071760 MS.gene82233:CDS 65.0%
GCGGTTGCGGCAGCGTTTGT+GGG + chr4.2:75071873-75071892 MS.gene82233:CDS 65.0%
GGAGGGATCAGAGAGGGCGA+GGG + chr4.2:75071923-75071942 MS.gene82233:CDS 65.0%
GGGAGCGAAGAGGGACGCAT+TGG + chr4.2:75071701-75071720 MS.gene82233:CDS 65.0%
TGCGTCCCTCTTCGCTCCCT+CGG - chr4.2:75071700-75071719 None:intergenic 65.0%
TGGACCCGAGGGAGCGAAGA+GGG + chr4.2:75071692-75071711 MS.gene82233:CDS 65.0%
TGGGGCCGTGGAGATGACAG+CGG + chr4.2:75071779-75071798 MS.gene82233:CDS 65.0%
! AAGATCGGGTGCCACGTGGG+AGG + chr4.2:75071557-75071576 MS.gene82233:CDS 65.0%
! GTTGGTAGAGAGTGGGGCCG+TGG + chr4.2:75071767-75071786 MS.gene82233:CDS 65.0%
! TGGTGCAACGACGGCGTCGA+CGG + chr4.2:75070888-75070907 MS.gene82233:CDS 65.0%
!! CGGTGGGGAGTGTTAGAGGG+AGG + chr4.2:75072054-75072073 MS.gene82233:CDS 65.0%
AGAGAGGGCGAGGGAGAGCG+CGG + chr4.2:75071932-75071951 MS.gene82233:CDS 70.0%
CGCAGCGGATAGGGAGACGG+TGG + chr4.2:75071740-75071759 MS.gene82233:CDS 70.0%
GCGTCCCTCTTCGCTCCCTC+GGG - chr4.2:75071699-75071718 None:intergenic 70.0%
GGCGGTAATGCCGGAGGAGG+CGG + chr4.2:75071809-75071828 MS.gene82233:CDS 70.0%
GGCGGTTGCGGCAGCGTTTG+TGG + chr4.2:75071872-75071891 MS.gene82233:CDS 70.0%
GGGAGGGATCAGAGAGGGCG+AGG + chr4.2:75071922-75071941 MS.gene82233:CDS 70.0%
GGGCCGTGGAGATGACAGCG+GGG + chr4.2:75071781-75071800 MS.gene82233:CDS 70.0%
GGGGCCGTGGAGATGACAGC+GGG + chr4.2:75071780-75071799 MS.gene82233:CDS 70.0%
! TGGGCGGCGGGTTTGGATGG+TGG + chr4.2:75072113-75072132 MS.gene82233:CDS 70.0%
!! GGTGCAACGACGGCGTCGAC+GGG + chr4.2:75070889-75070908 MS.gene82233:CDS 70.0%
! ACGTCGGTGGGCGGCGGGTT+TGG + chr4.2:75072106-75072125 MS.gene82233:CDS 75.0%
! CGGTGGGCGGCGGGTTTGGA+TGG + chr4.2:75072110-75072129 MS.gene82233:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 75070112 75072223 75070112 ID=MS.gene82233
chr4.2 mRNA 75070112 75072223 75070112 ID=MS.gene82233.t1;Parent=MS.gene82233
chr4.2 exon 75070112 75072223 75070112 ID=MS.gene82233.t1.exon1;Parent=MS.gene82233.t1
chr4.2 CDS 75070112 75072223 75070112 ID=cds.MS.gene82233.t1;Parent=MS.gene82233.t1
Gene Sequence

>MS.gene82233

ATGGCAATATCACCTCAAACATTTCCAACTACTAAACCACCATCATCAGAAACGGCGTCGTTTATCACCCCAAATATCTCTAACTTTAACCTCCTCCATTCTCTATTACACCTCACAAACCAAATATGTTCCTTAAACGCAGCTCCATGGTGTCGTTTCCTTTTAAACAAAATTTCCTCTTCCACTATACGTAAAACGCACCTCCTCGGTGTCGTCTTCGATGACCTAATTCGTAATTCAATATCAGTTTCAGCTTCGAATTCAACTCGTCAAAATTCTTACGTTCTTTGTTTAGAAGAAATGTATATTGTGCTTCATAGGATTAAAACTCTGCTCGAAGATTGTTCTAATGGAAGCAAATTTTCTATTCTTATGCAGATTGAAACTGTTGCTGATAATTTCAACAAATTAACAGGTGAATTATCAACTCTTCTTGATGTTTTTCCAATTCAAGAAATGAACTTGAATGATGATGTATTGGAGCTTGTTCTTCTGATTAGAAAACAGTGTTCAGAAACGAAGAGTTTGTTAGGTGAAGAACAAATTCATCTCCGAAACGACGTTGTTTCTGTTCTAGAAGGAATCAAGAGAGAAATTGTTCCAAAAGATGAAAAATTATCTTCAATTTTTGAGAAATTGGAGATTCAAGATTCTTCAAGTTGTAGAAGTGAGATTGAAAATCTTCAAGAAGAAATTCAGAATCGGAGCGAAGAACAATCGAAATCAGAGATTGTTGCGTTGATCGGACTTGTTCGTTATGCGAAGTGCGTTCTTTTTGGTGCAACGACGGCGTCGACGGGAAATGATGGCCGTTTACGGCGGAATCAGTCGTTGGAATTGGTGGTTCCGGCGGATTACCGGTGTCCGATAAGTCTTGAACTGATGCGGGATCCTGTCGTCGTGGCAACAGGACAGACGTACGATCGTGTTTCGATTAAGCTTTGGATGGATTCTGGGCACAACACGTGTCCTAAAACAGGTCAAACCCTGGCCCACACGGAGTTAATACCTAACCATGCTTTGAGAAATATGATAGCTATTTGGTGCAAAGAACAGAAAATTCCATTTGAAGGTGAAACGGTAGCCGTTAAAGTTAACGGTGGTATGACAAATAAGACAGCTTTGGAAGCAACGAGAATGACGGCGGCGTTTTTAGTCAACAAGTTGAAGTTGACTAACGGTTCTTTAGCGTCGATGGAAGAGACAAATGGCGTTGTTTATGAGCTTCGGGTTTTGGCGAAATCGGATTCAGAGAGTCGAGCTTGTATAGCTGAAGCTGGAGCTATAGAGATTTTGGTTCGGTTTTTAAGTTACGAAGTTGGTTCGCAAAATCCAAGCCTACAGGTTAATGCAGTTACAACGATATTAAATCTCTCGATACTTGAAGCGAATAAGACAAGGATAATGGAGACTGAAGGTGTTTTAAGTGGTGTGGCAGAGGTTTTAAGATCGGGTGCCACGTGGGAGGCTAAAGCAAATGCGGCGGCTACTGTGTTTAGTTTAACTGGAGTGGTTGCTTTTAGAAAGCGGTTAGGTAGGAAGACACGAATCGTTAGTGGATTGGTTGAGTTGGCGAAAAATGGACCCGAGGGAGCGAAGAGGGACGCATTGGCGGCAATTTTGCATCTCGCAGCGGATAGGGAGACGGTGGGAAGGTTGGTAGAGAGTGGGGCCGTGGAGATGACAGCGGGGATTATGGCGGTAATGCCGGAGGAGGCGGTGACGATACTTGAAGCAGTGGTTAAGCGTGGAGGATTAGTGGCGGTTGCGGCAGCGTTTGTGGGGATAAAAAAATTGGGGACGGTGTTAAGGGAGGGATCAGAGAGGGCGAGGGAGAGCGCGGCCGCGACACTTGTGACAATGTGTAGGAAAGGTGGATCAGAGATTGTGGCGGAGCTTGCGGCAATTCACGGTGTGGAAAGGGTGATTTGGGAGTTAATGGCGGTGGGGAGTGTTAGAGGGAGGAGGAAGGCAGCGACGTTGTTGAGGATTTTACGTCGGTGGGCGGCGGGTTTGGATGGTGGTGAAACGGAGGGATTTACATCAACAAACACTTTTGTTAGTACATCAACTACCATGGTTGTTCCAACTACAACTTCATTAGCACAGTAA
Protein sequence

>MS.gene82233.t1

MAISPQTFPTTKPPSSETASFITPNISNFNLLHSLLHLTNQICSLNAAPWCRFLLNKISSSTIRKTHLLGVVFDDLIRNSISVSASNSTRQNSYVLCLEEMYIVLHRIKTLLEDCSNGSKFSILMQIETVADNFNKLTGELSTLLDVFPIQEMNLNDDVLELVLLIRKQCSETKSLLGEEQIHLRNDVVSVLEGIKREIVPKDEKLSSIFEKLEIQDSSSCRSEIENLQEEIQNRSEEQSKSEIVALIGLVRYAKCVLFGATTASTGNDGRLRRNQSLELVVPADYRCPISLELMRDPVVVATGQTYDRVSIKLWMDSGHNTCPKTGQTLAHTELIPNHALRNMIAIWCKEQKIPFEGETVAVKVNGGMTNKTALEATRMTAAFLVNKLKLTNGSLASMEETNGVVYELRVLAKSDSESRACIAEAGAIEILVRFLSYEVGSQNPSLQVNAVTTILNLSILEANKTRIMETEGVLSGVAEVLRSGATWEAKANAAATVFSLTGVVAFRKRLGRKTRIVSGLVELAKNGPEGAKRDALAAILHLAADRETVGRLVESGAVEMTAGIMAVMPEEAVTILEAVVKRGGLVAVAAAFVGIKKLGTVLREGSERARESAAATLVTMCRKGGSEIVAELAAIHGVERVIWELMAVGSVRGRRKAATLLRILRRWAAGLDGGETEGFTSTNTFVSTSTTMVVPTTTSLAQ