Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82685.t1 | XP_024634584.1 | 80.7 | 181 | 35 | 0 | 15 | 195 | 1 | 181 | 1.70E-80 | 308.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82685.t1 | G7IXJ0 | 80.7 | 181 | 35 | 0 | 15 | 195 | 1 | 181 | 1.2e-80 | 308.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054467 | MS.gene82685 | 0.803643 | 3.16E-49 | -1.69E-46 |
| MS.gene054801 | MS.gene82685 | 0.803866 | 2.84E-49 | -1.69E-46 |
| MS.gene055926 | MS.gene82685 | 0.815086 | 1.11E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene82685 | 0.802518 | 5.40E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82685.t1 | MTR_3g051020 | 80.663 | 181 | 35 | 0 | 15 | 195 | 1 | 181 | 8.04e-110 | 313 |
| MS.gene82685.t1 | MTR_3g050500 | 72.527 | 182 | 44 | 4 | 17 | 194 | 4 | 183 | 2.10e-83 | 261 |
| MS.gene82685.t1 | MTR_3g051060 | 47.273 | 165 | 77 | 3 | 40 | 194 | 28 | 192 | 3.89e-40 | 145 |
| MS.gene82685.t1 | MTR_3g058550 | 50.365 | 137 | 58 | 2 | 68 | 194 | 5 | 141 | 8.68e-39 | 132 |
| MS.gene82685.t1 | MTR_5g085340 | 41.875 | 160 | 83 | 3 | 42 | 194 | 25 | 181 | 1.18e-31 | 120 |
| MS.gene82685.t1 | MTR_5g085350 | 44.848 | 165 | 77 | 5 | 42 | 194 | 25 | 187 | 8.44e-31 | 118 |
| MS.gene82685.t1 | MTR_5g085350 | 44.848 | 165 | 77 | 5 | 42 | 194 | 25 | 187 | 9.48e-31 | 118 |
| MS.gene82685.t1 | MTR_5g085360 | 40.994 | 161 | 84 | 3 | 42 | 194 | 25 | 182 | 2.77e-30 | 117 |
| MS.gene82685.t1 | MTR_2g020510 | 35.065 | 154 | 94 | 3 | 45 | 195 | 14 | 164 | 6.07e-17 | 78.6 |
| MS.gene82685.t1 | MTR_2g020450 | 35.065 | 154 | 94 | 3 | 45 | 195 | 14 | 164 | 6.77e-16 | 75.5 |
| MS.gene82685.t1 | MTR_2g020470 | 34.416 | 154 | 95 | 3 | 45 | 195 | 14 | 164 | 3.61e-15 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTAGAGTCCCAAATAAAA+AGG | 0.211133 | 3.1:-37591715 | MS.gene82685:CDS |
| ACATCTTCATCTTCATTTGC+AGG | 0.309830 | 3.1:+37592288 | None:intergenic |
| ATAGCAAGATTGAGATCATT+TGG | 0.331887 | 3.1:+37591621 | None:intergenic |
| CTGTGCTTGCACGCCATAAA+AGG | 0.343353 | 3.1:-37591671 | MS.gene82685:CDS |
| CGTAGAATTAGAATTCATTT+CGG | 0.359183 | 3.1:+37592317 | None:intergenic |
| TAGCAAGATTGAGATCATTT+GGG | 0.422742 | 3.1:+37591622 | None:intergenic |
| CTTCACCTTGCAGCATATAG+AGG | 0.427598 | 3.1:-37591792 | MS.gene82685:CDS |
| GTACCTTATGACTATCTAGA+TGG | 0.447562 | 3.1:-37592267 | MS.gene82685:intron |
| AAGAGGAGTATCATCTCTTT+CGG | 0.469914 | 3.1:+37591812 | None:intergenic |
| ACCGAACATTTCTCTATGAA+TGG | 0.471652 | 3.1:+37591566 | None:intergenic |
| CGAGTTCCGCGAGTGGGTAA+TGG | 0.492725 | 3.1:-37592015 | MS.gene82685:CDS |
| GTGGGTAATGGAAGACGATT+GGG | 0.496127 | 3.1:-37592003 | MS.gene82685:CDS |
| GCCATTCATAGAGAAATGTT+CGG | 0.497959 | 3.1:-37591567 | MS.gene82685:CDS |
| GAACCTTCCGAAATGAATCC+TGG | 0.500850 | 3.1:-37592366 | None:intergenic |
| AGTGGGTAATGGAAGACGAT+TGG | 0.517975 | 3.1:-37592004 | MS.gene82685:CDS |
| ATGAATGGCAACGTGAAGAA+TGG | 0.524484 | 3.1:+37591581 | None:intergenic |
| AGTATGTGTAAATGTATCGT+TGG | 0.546925 | 3.1:-37591762 | MS.gene82685:CDS |
| CTATATGCTGCAAGGTGAAG+AGG | 0.554034 | 3.1:+37591795 | None:intergenic |
| AGCTGCCGAGTTCCGCGAGT+GGG | 0.561779 | 3.1:-37592021 | MS.gene82685:CDS |
| ATACCATCTAGATAGTCATA+AGG | 0.565626 | 3.1:+37592264 | None:intergenic |
| TTCATTTCGGAATCATCTTG+AGG | 0.572627 | 3.1:+37592330 | None:intergenic |
| CATTACCCACTCGCGGAACT+CGG | 0.582026 | 3.1:+37592016 | None:intergenic |
| AAATTGATGTAGGTAAAGAA+AGG | 0.583759 | 3.1:+37591647 | None:intergenic |
| TGAATCCTCTATATGCTGCA+AGG | 0.593278 | 3.1:+37591787 | None:intergenic |
| CAATCTTGCTATGAATAACG+AGG | 0.598611 | 3.1:-37591610 | MS.gene82685:CDS |
| TGAGGCTGAATCATAGAACC+AGG | 0.620158 | 3.1:+37592348 | None:intergenic |
| GTAAAGCATAATGATCAAAG+TGG | 0.624361 | 3.1:-37591849 | MS.gene82685:CDS |
| TAGCTGCCGAGTTCCGCGAG+TGG | 0.684675 | 3.1:-37592022 | MS.gene82685:CDS |
| CGTCTTCCATTACCCACTCG+CGG | 0.694968 | 3.1:+37592009 | None:intergenic |
| AAGAGGAACAGCATTACATG+TGG | 0.700334 | 3.1:-37591922 | MS.gene82685:CDS |
| AATCTTGCTATGAATAACGA+GGG | 0.709353 | 3.1:-37591609 | MS.gene82685:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTAATCACAAAAAATGT+CGG | + | chr3.1:37591770-37591789 | None:intergenic | 15.0% |
| !! | TTTCACAAGATAAAAATAAA+GGG | - | chr3.1:37591972-37591991 | MS.gene82685:CDS | 15.0% |
| !!! | TTTTCACAAGATAAAAATAA+AGG | - | chr3.1:37591971-37591990 | MS.gene82685:CDS | 15.0% |
| !! | AGTTATTGTCACAAAAAAAT+TGG | + | chr3.1:37591862-37591881 | None:intergenic | 20.0% |
| ! | AAATGTATCGTTGGAAAAAA+TGG | - | chr3.1:37592164-37592183 | MS.gene82685:intron | 25.0% |
| ! | AAATTGATGTAGGTAAAGAA+AGG | + | chr3.1:37592273-37592292 | None:intergenic | 25.0% |
| ! | AAGATAAAAATAAAGGGAAG+AGG | - | chr3.1:37591978-37591997 | MS.gene82685:CDS | 25.0% |
| ! | ATTAGAGTCCCAAATAAAAA+GGG | - | chr3.1:37592203-37592222 | MS.gene82685:intron | 25.0% |
| ! | CGTAGAATTAGAATTCATTT+CGG | + | chr3.1:37591603-37591622 | None:intergenic | 25.0% |
| ! | TAAAGAGCTTGATAGATATA+AGG | + | chr3.1:37591690-37591709 | None:intergenic | 25.0% |
| AGTATGTGTAAATGTATCGT+TGG | - | chr3.1:37592155-37592174 | MS.gene82685:intron | 30.0% | |
| ATACCATCTAGATAGTCATA+AGG | + | chr3.1:37591656-37591675 | None:intergenic | 30.0% | |
| ATAGCAAGATTGAGATCATT+TGG | + | chr3.1:37592299-37592318 | None:intergenic | 30.0% | |
| CATTTGGGAAAAATTGATGT+AGG | + | chr3.1:37592283-37592302 | None:intergenic | 30.0% | |
| GATTAGAGTCCCAAATAAAA+AGG | - | chr3.1:37592202-37592221 | MS.gene82685:intron | 30.0% | |
| GTAAAGCATAATGATCAAAG+TGG | - | chr3.1:37592068-37592087 | MS.gene82685:intron | 30.0% | |
| TAGCAAGATTGAGATCATTT+GGG | + | chr3.1:37592298-37592317 | None:intergenic | 30.0% | |
| ! | AATCTTGCTATGAATAACGA+GGG | - | chr3.1:37592308-37592327 | MS.gene82685:CDS | 30.0% |
| !! | GTGTTTCTCCCTTTTTATTT+GGG | + | chr3.1:37592214-37592233 | None:intergenic | 30.0% |
| ACATCTTCATCTTCATTTGC+AGG | + | chr3.1:37591632-37591651 | None:intergenic | 35.0% | |
| ACCGAACATTTCTCTATGAA+TGG | + | chr3.1:37592354-37592373 | None:intergenic | 35.0% | |
| GCCATTCATAGAGAAATGTT+CGG | - | chr3.1:37592350-37592369 | MS.gene82685:CDS | 35.0% | |
| GTACCTTATGACTATCTAGA+TGG | - | chr3.1:37591650-37591669 | MS.gene82685:CDS | 35.0% | |
| TCGTTGGAAAAAATGGTGAA+AGG | - | chr3.1:37592171-37592190 | MS.gene82685:intron | 35.0% | |
| TTCATTTCGGAATCATCTTG+AGG | + | chr3.1:37591590-37591609 | None:intergenic | 35.0% | |
| ! | AAGTTTTACTCTCTCTTCGT+CGG | + | chr3.1:37592033-37592052 | None:intergenic | 35.0% |
| ! | CAATCTTGCTATGAATAACG+AGG | - | chr3.1:37592307-37592326 | MS.gene82685:CDS | 35.0% |
| ! | GGTAAAGAAAGGTCCTTTTA+TGG | + | chr3.1:37592262-37592281 | None:intergenic | 35.0% |
| ! | GGTGTTTCTCCCTTTTTATT+TGG | + | chr3.1:37592215-37592234 | None:intergenic | 35.0% |
| !! | AAGAGGAGTATCATCTCTTT+CGG | + | chr3.1:37592108-37592127 | None:intergenic | 35.0% |
| AAGAGGAACAGCATTACATG+TGG | - | chr3.1:37591995-37592014 | MS.gene82685:CDS | 40.0% | |
| ATGAATGGCAACGTGAAGAA+TGG | + | chr3.1:37592339-37592358 | None:intergenic | 40.0% | |
| TGAATCCTCTATATGCTGCA+AGG | + | chr3.1:37592133-37592152 | None:intergenic | 40.0% | |
| TGCAAGCACAGCACAAAAAA+GGG | + | chr3.1:37592237-37592256 | None:intergenic | 40.0% | |
| CTATATGCTGCAAGGTGAAG+AGG | + | chr3.1:37592125-37592144 | None:intergenic | 45.0% | |
| CTTCACCTTGCAGCATATAG+AGG | - | chr3.1:37592125-37592144 | MS.gene82685:intron | 45.0% | |
| GCAAGCACAGCACAAAAAAG+GGG | + | chr3.1:37592236-37592255 | None:intergenic | 45.0% | |
| GTGCAAGCACAGCACAAAAA+AGG | + | chr3.1:37592238-37592257 | None:intergenic | 45.0% | |
| TGAGGCTGAATCATAGAACC+AGG | + | chr3.1:37591572-37591591 | None:intergenic | 45.0% | |
| !! | AGTGGGTAATGGAAGACGAT+TGG | - | chr3.1:37591913-37591932 | MS.gene82685:CDS | 45.0% |
| !! | GTGGGTAATGGAAGACGATT+GGG | - | chr3.1:37591914-37591933 | MS.gene82685:CDS | 45.0% |
| !!! | ATTTTTTATTTTATTTTTCA+AGG | + | chr3.1:37591743-37591762 | None:intergenic | 5.0% |
| CTGTGCTTGCACGCCATAAA+AGG | - | chr3.1:37592246-37592265 | MS.gene82685:intron | 50.0% | |
| CATTACCCACTCGCGGAACT+CGG | + | chr3.1:37591904-37591923 | None:intergenic | 55.0% | |
| CGTCTTCCATTACCCACTCG+CGG | + | chr3.1:37591911-37591930 | None:intergenic | 55.0% | |
| CGAGTTCCGCGAGTGGGTAA+TGG | - | chr3.1:37591902-37591921 | MS.gene82685:CDS | 60.0% | |
| AGCTGCCGAGTTCCGCGAGT+GGG | - | chr3.1:37591896-37591915 | MS.gene82685:CDS | 65.0% | |
| TAGCTGCCGAGTTCCGCGAG+TGG | - | chr3.1:37591895-37591914 | MS.gene82685:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 37591563 | 37592376 | 37591563 | ID=MS.gene82685 |
| chr3.1 | mRNA | 37591563 | 37592376 | 37591563 | ID=MS.gene82685.t1;Parent=MS.gene82685 |
| chr3.1 | exon | 37592268 | 37592376 | 37592268 | ID=MS.gene82685.t1.exon1;Parent=MS.gene82685.t1 |
| chr3.1 | CDS | 37592268 | 37592376 | 37592268 | ID=cds.MS.gene82685.t1;Parent=MS.gene82685.t1 |
| chr3.1 | exon | 37591563 | 37592041 | 37591563 | ID=MS.gene82685.t1.exon2;Parent=MS.gene82685.t1 |
| chr3.1 | CDS | 37591563 | 37592041 | 37591563 | ID=cds.MS.gene82685.t1;Parent=MS.gene82685.t1 |
| Gene Sequence |
| Protein sequence |
