AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83325

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83325.t1 MTR_2g054940 99.609 511 2 0 1 511 1 511 0.0 1059
MS.gene83325.t1 MTR_7g081695 96.040 505 8 1 1 505 1 493 0.0 994
MS.gene83325.t1 MTR_4g113280 87.550 498 51 1 14 511 3 489 0.0 911
MS.gene83325.t1 MTR_1g076430 75.889 506 122 0 6 511 7 512 0.0 826
MS.gene83325.t1 MTR_1g041085 77.485 493 111 0 19 511 26 518 0.0 816
MS.gene83325.t1 MTR_4g070270 68.635 491 153 1 15 505 13 502 0.0 713
MS.gene83325.t1 MTR_7g118170 65.737 502 157 6 17 506 31 529 0.0 668
MS.gene83325.t1 MTR_1g110930 63.136 491 180 1 18 508 4 493 0.0 639
MS.gene83325.t1 MTR_3g105550 58.084 501 198 4 17 505 36 536 0.0 627
MS.gene83325.t1 MTR_5g056510 60.081 491 195 1 18 508 4 493 0.0 617
MS.gene83325.t1 MTR_6g074880 60.917 458 173 2 56 507 13 470 0.0 578
MS.gene83325.t1 MTR_4g054630 59.477 459 179 3 53 505 9 466 0.0 567
MS.gene83325.t1 MTR_4g054415 62.919 418 154 1 89 505 8 425 0.0 560
MS.gene83325.t1 MTR_6g032965 61.818 385 147 0 122 506 1 385 0.0 514
MS.gene83325.t1 MTR_2g013740 53.734 482 209 5 19 497 33 503 9.36e-172 494
MS.gene83325.t1 MTR_7g118160 55.198 404 137 3 102 503 56 417 7.88e-160 461
MS.gene83325.t1 MTR_2g436480 49.890 455 210 7 63 505 3 451 1.55e-157 456
MS.gene83325.t1 MTR_2g007020 52.683 410 191 3 98 506 45 452 6.72e-157 454
MS.gene83325.t1 MTR_1g102290 47.059 408 215 1 97 503 77 484 6.63e-136 402
MS.gene83325.t1 MTR_3g072940 46.472 411 218 2 94 503 74 483 4.98e-131 389
MS.gene83325.t1 MTR_3g072980 46.472 411 218 2 94 503 74 483 5.20e-131 389
MS.gene83325.t1 MTR_6g051440 46.287 404 215 2 101 503 80 482 2.48e-120 362
MS.gene83325.t1 MTR_1g115560 39.954 433 227 7 98 504 28 453 3.36e-109 333
MS.gene83325.t1 MTR_4g062270 39.817 437 245 6 76 502 5 433 4.67e-107 327
MS.gene83325.t1 MTR_4g062190 38.170 448 250 5 72 502 5 442 4.70e-106 325
MS.gene83325.t1 MTR_2g096210 39.813 427 226 6 98 502 27 444 9.12e-105 322
MS.gene83325.t1 MTR_4g062170 38.514 444 253 5 72 502 5 441 1.52e-102 316
MS.gene83325.t1 MTR_4g054260 58.203 256 89 2 251 505 87 325 8.50e-100 304
MS.gene83325.t1 MTR_4g053905 67.296 159 52 0 347 505 1 159 7.57e-74 231
MS.gene83325.t1 MTR_4g054265 50.893 112 55 0 87 198 6 117 3.52e-34 125
MS.gene83325.t1 MTR_7g086260 59.615 52 21 0 345 396 25 76 1.22e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83325.t1 AT2G26640 82.711 509 87 1 3 511 2 509 0.0 900
MS.gene83325.t1 AT1G04220 72.079 505 132 5 15 511 18 521 0.0 763
MS.gene83325.t1 AT5G43760 74.239 493 121 3 25 511 34 526 0.0 735
MS.gene83325.t1 AT1G19440 68.712 489 152 1 15 503 25 512 0.0 715
MS.gene83325.t1 AT2G16280 67.894 489 156 1 17 505 23 510 0.0 705
MS.gene83325.t1 AT1G01120 62.828 495 178 3 17 505 32 526 0.0 660
MS.gene83325.t1 AT1G68530 61.837 490 186 1 19 508 6 494 0.0 644
MS.gene83325.t1 AT4G34510 63.522 477 167 3 30 505 15 485 0.0 639
MS.gene83325.t1 AT1G25450 61.224 490 189 1 19 508 1 489 0.0 629
MS.gene83325.t1 AT2G26250 55.238 525 202 5 8 503 28 548 0.0 601
MS.gene83325.t1 AT4G34250 57.851 484 197 3 28 505 5 487 0.0 573
MS.gene83325.t1 AT4G34520 58.025 486 192 3 31 505 6 490 0.0 573
MS.gene83325.t1 AT2G15090 58.511 470 184 5 45 505 12 479 0.0 558
MS.gene83325.t1 AT2G46720 60.142 424 164 2 87 505 42 465 0.0 553
MS.gene83325.t1 AT3G10280 58.962 424 162 3 87 505 42 458 0.0 533
MS.gene83325.t1 AT1G71160 50.943 424 204 4 82 503 25 446 1.12e-158 459
MS.gene83325.t1 AT1G68530 57.849 344 145 0 19 362 6 349 1.40e-145 422
MS.gene83325.t1 AT5G49070 49.412 425 207 4 87 506 35 456 5.90e-145 424
MS.gene83325.t1 AT3G52160 44.969 487 197 5 20 494 22 449 1.31e-141 415
MS.gene83325.t1 AT3G52160 50.959 365 148 2 130 494 3 336 5.77e-131 384
MS.gene83325.t1 AT5G04530 39.810 422 243 5 89 502 19 437 3.66e-107 328
MS.gene83325.t1 AT2G28630 40.515 427 225 7 98 502 27 446 8.82e-107 327
MS.gene83325.t1 AT2G28630 40.515 427 225 7 98 502 27 446 8.82e-107 327
MS.gene83325.t1 AT1G07720 40.758 422 232 4 98 502 27 447 1.10e-105 324
MS.gene83325.t1 AT1G07720 40.758 422 232 4 98 502 70 490 1.84e-105 325
MS.gene83325.t1 AT5G49070 44.444 279 154 1 87 364 35 313 5.27e-80 253
MS.gene83325.t1 AT4G34850 25.483 259 149 10 236 482 91 317 2.63e-12 68.2
MS.gene83325.t1 AT4G34850 25.483 259 149 10 236 482 164 390 5.45e-12 68.2

Find 134 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATGATATCTATGATATC+TGG 0.226248 2.2:-30050554 MS.gene83325:CDS
ATGCACTAAGAGGATATTTA+TGG 0.261715 2.2:-30050391 MS.gene83325:CDS
TACTTTATCAAAAGATCTTA+TGG 0.267099 2.2:-30049722 MS.gene83325:CDS
AGAATGACACTTTATCGTTT+TGG 0.268393 2.2:-30049454 MS.gene83325:CDS
GGTGGGATGGGATGCAGTGC+TGG 0.280642 2.2:-30050042 MS.gene83325:CDS
CTGCAATTCTACTTTCTAAC+AGG 0.282789 2.2:-30049867 MS.gene83325:CDS
CGATGAGATCCACCAGTTTC+CGG 0.286351 2.2:-30049257 MS.gene83325:CDS
TTCCTAGCTTCTTTCATTGA+AGG 0.317480 2.2:+30050243 None:intergenic
ATTTAGATGGCAGAAATTCT+TGG 0.321018 2.2:+30049226 None:intergenic
ATCAGAATTCCAATGTCTTT+AGG 0.328553 2.2:+30050159 None:intergenic
AGCTTGAAATCCGGAATATA+AGG 0.330746 2.2:+30049574 None:intergenic
GGTGTGGACCAATTGGTATT+TGG 0.335798 2.2:+30049825 None:intergenic
TGGCAAATAGCATTTGGTTC+TGG 0.340846 2.2:-30049352 MS.gene83325:CDS
AAAACAAACATTACCACGTT+GGG 0.342115 2.2:-30049679 MS.gene83325:CDS
GTGCATTTGCGAGACTCTTC+CGG 0.354359 2.2:+30050408 None:intergenic
TCCTTTCTTGATCCTTCCCT+TGG 0.368172 2.2:+30049384 None:intergenic
AAAGATCTTATGGCCGTTGC+AGG 0.375775 2.2:-30049712 MS.gene83325:CDS
TTCGAATTGTTTGTTCCGTA+TGG 0.380907 2.2:-30049896 MS.gene83325:CDS
AGCTTCTTTCATTGAAGGAT+TGG 0.386247 2.2:+30050248 None:intergenic
TTTAAACTACATCATTTAGA+TGG 0.399579 2.2:+30049213 None:intergenic
ACAACCAACGCATACGAGTT+AGG 0.410167 2.2:+30049976 None:intergenic
AAGCTTGGATCGATCGTTCC+CGG 0.410332 2.2:+30049921 None:intergenic
AGGACGAGTCATAAAGTAAA+AGG 0.410912 2.2:+30050467 None:intergenic
GCTTCTTTCATTGAAGGATT+GGG 0.411634 2.2:+30050249 None:intergenic
TTGGAACATCGACCGGAAAC+TGG 0.414672 2.2:+30049245 None:intergenic
GAAGATAAAGCCTTATATTC+CGG 0.417524 2.2:-30049584 MS.gene83325:CDS
GCGCAGTATGGAAGGCTCTA+AGG 0.417809 2.2:-30049315 MS.gene83325:CDS
AGAACATGGCAAATAGCATT+TGG 0.419756 2.2:-30049358 MS.gene83325:CDS
GAAGCGGAGGCAGTTATGTT+TGG 0.419896 2.2:-30050219 MS.gene83325:CDS
TCAAGTGCAACAGCGCAGTA+TGG 0.422431 2.2:-30049327 MS.gene83325:CDS
CGATAAAGTGTCATTCTTGA+CGG 0.427252 2.2:+30049460 None:intergenic
GGAATGCTTAGAACAGCTTC+TGG 0.428265 2.2:+30050273 None:intergenic
TGGAGAACATCACGTTGAAT+TGG 0.436866 2.2:-30049948 MS.gene83325:CDS
ATCCTTGAGAGATCTGGTCT+TGG 0.439851 2.2:-30050312 MS.gene83325:CDS
CGAAAGATCCTTGAGAGATC+TGG 0.441571 2.2:-30050318 MS.gene83325:CDS
CTGTTCTTCGCTACGTTGGT+TGG 0.446813 2.2:-30049625 MS.gene83325:CDS
TGCGAACGGTGTGGACCAAT+TGG 0.448066 2.2:+30049818 None:intergenic
GAAGCTGTCTCCATGGCATA+TGG 0.449190 2.2:-30049485 MS.gene83325:CDS
GGTACATGCCATGAGTGATT+AGG 0.451469 2.2:+30050631 None:intergenic
CAGTTCGCTTTGGTACGAAT+TGG 0.452777 2.2:-30049419 MS.gene83325:CDS
GTGGGATGGGATGCAGTGCT+GGG 0.466651 2.2:-30050041 MS.gene83325:CDS
CCTCTTTCTTTCACCACTTG+TGG 0.470663 2.2:-30050610 MS.gene83325:CDS
CAATGATATCTATGATATCT+GGG 0.474517 2.2:-30050553 MS.gene83325:CDS
CGACGACGATGGCGCTAACA+AGG 0.474648 2.2:+30050716 None:intergenic
GAGAAGATCCAAATACCAAT+TGG 0.476259 2.2:-30049833 MS.gene83325:CDS
ACACCTCTAGCAGTTCGCTT+TGG 0.480585 2.2:-30049429 MS.gene83325:CDS
TCGAATTGTTTGTTCCGTAT+GGG 0.486272 2.2:-30049895 MS.gene83325:CDS
ATCTCTCAAGGATCTTTCGT+TGG 0.488205 2.2:+30050322 None:intergenic
TTTACTTTATGACTCGTCCT+AGG 0.488605 2.2:-30050464 MS.gene83325:CDS
GAAGACGACAATGGCAAAGT+TGG 0.504336 2.2:-30049748 MS.gene83325:CDS
AACATAATGAGTTACAATCT+TGG 0.513615 2.2:-30050063 MS.gene83325:CDS
ATTGTTTGTTCCGTATGGGA+GGG 0.513671 2.2:-30049891 MS.gene83325:CDS
TCAAAAGCTAGCTTGAAATC+CGG 0.514010 2.2:+30049565 None:intergenic
AGCGAAGAACAGCAACTGTT+CGG 0.515852 2.2:+30049636 None:intergenic
ATCTAAAACAGCTCTTCCAC+CGG 0.515874 2.2:+30049525 None:intergenic
GCCAAGGGAAGGATCAAGAA+AGG 0.516116 2.2:-30049385 MS.gene83325:CDS
GAGTTACAATCTTGGTGGGA+TGG 0.516163 2.2:-30050055 MS.gene83325:CDS
GAAATTCTTGGAACATCGAC+CGG 0.516259 2.2:+30049238 None:intergenic
ATCACGTTGAATTGGTATCC+CGG 0.518078 2.2:-30049940 MS.gene83325:CDS
AGCTTGGATCGATCGTTCCC+GGG 0.518481 2.2:+30049922 None:intergenic
TTGTTTGTTCCGTATGGGAG+GGG 0.520242 2.2:-30049890 MS.gene83325:CDS
CAAACAATTCGAAACAAGCT+TGG 0.524897 2.2:+30049906 None:intergenic
CTCCAAGACCAGATCTCTCA+AGG 0.527098 2.2:+30050310 None:intergenic
GTGCAACAGCGCAGTATGGA+AGG 0.529765 2.2:-30049323 MS.gene83325:CDS
CCACACCGTTCGCACTAACA+AGG 0.531499 2.2:-30049809 MS.gene83325:CDS
CTACAAAAGAGAAGAACCCT+TGG 0.534529 2.2:-30049282 MS.gene83325:CDS
GAGGGCGAGTGGTTTCTTCA+CGG 0.536357 2.2:-30050361 MS.gene83325:CDS
AGTCTCGCAAATGCACTAAG+AGG 0.536367 2.2:-30050401 MS.gene83325:CDS
ATTGCAGAGAGTGACGGAGT+TGG 0.537030 2.2:+30050114 None:intergenic
AATTGTTTGTTCCGTATGGG+AGG 0.537893 2.2:-30049892 MS.gene83325:CDS
TCTTGCTAAAGAGCTTCTCC+AGG 0.541294 2.2:-30050004 MS.gene83325:CDS
GTATGCGTTGGTTGTTAGCA+TGG 0.541573 2.2:-30049968 MS.gene83325:CDS
GATATCATAGATATCATTGA+GGG 0.541632 2.2:+30050557 None:intergenic
GCAACTGTTCGGACGTTGGA+AGG 0.547179 2.2:+30049647 None:intergenic
TTTCAAGGCATCACCTGCAA+CGG 0.548387 2.2:+30049699 None:intergenic
CCACAAGTGGTGAAAGAAAG+AGG 0.552043 2.2:+30050610 None:intergenic
TCTACTTTCTAACAGGGGAT+CGG 0.553751 2.2:-30049860 MS.gene83325:CDS
GAACATTTCTGCATTCATGC+CGG 0.553989 2.2:-30049544 MS.gene83325:CDS
GAGAGTCGAGCAAATAATAA+CGG 0.554120 2.2:+30050506 None:intergenic
AGTTACAATCTTGGTGGGAT+GGG 0.556990 2.2:-30050054 MS.gene83325:CDS
AGCACCCTTGTTAGTGCGAA+CGG 0.557174 2.2:+30049804 None:intergenic
TGGTGGATCTCATCGATCCA+AGG 0.558878 2.2:+30049265 None:intergenic
TTAACAAGGTAGACTGGCCT+AGG 0.559143 2.2:+30050447 None:intergenic
TCATCCTAACTCGTATGCGT+TGG 0.563252 2.2:-30049980 MS.gene83325:CDS
ATTGGCTTACACGGAAGCCA+AGG 0.568482 2.2:-30049401 MS.gene83325:CDS
AAAACTTGAAGCTGTCTCCA+TGG 0.569815 2.2:-30049492 MS.gene83325:CDS
AAATTGAAGTATGTGAAGCT+TGG 0.574482 2.2:-30050663 MS.gene83325:CDS
GAATTGCAGCCCCTCCCATA+CGG 0.574490 2.2:+30049881 None:intergenic
GAAAACAAACATTACCACGT+TGG 0.574589 2.2:-30049680 MS.gene83325:CDS
AACAGCAACTGTTCGGACGT+TGG 0.575994 2.2:+30049643 None:intergenic
ACGGTAAAGCCTAAAGACAT+TGG 0.576657 2.2:-30050168 MS.gene83325:CDS
TTTCTAACAGGGGATCGGAT+AGG 0.576964 2.2:-30049855 MS.gene83325:CDS
GAGAAATTAACAAGGTAGAC+TGG 0.577191 2.2:+30050441 None:intergenic
CATACGAGTTAGGATGAACC+TGG 0.577273 2.2:+30049986 None:intergenic
TTGGTACGAATTGGCTTACA+CGG 0.578204 2.2:-30049410 MS.gene83325:CDS
TCACGTTGAATTGGTATCCC+GGG 0.579569 2.2:-30049939 MS.gene83325:CDS
TAATTTCTCGTGTTACAAGC+CGG 0.580512 2.2:-30050427 MS.gene83325:CDS
TATCATTACCTAATCACTCA+TGG 0.585224 2.2:-30050639 MS.gene83325:CDS
GGAAGGATCCGCGACGACGA+TGG 0.585504 2.2:+30050705 None:intergenic
TCTTTCATTGAAGGATTGGG+AGG 0.589318 2.2:+30050252 None:intergenic
CATTTCTGCATTCATGCCGG+TGG 0.590314 2.2:-30049541 MS.gene83325:CDS
ATCCTTCAATGAAAGAAGCT+AGG 0.592042 2.2:-30050245 MS.gene83325:CDS
ATGGATCACTCGAGGGCGAG+TGG 0.595528 2.2:-30050372 MS.gene83325:CDS
ATGATCATTGCAGAGAGTGA+CGG 0.597131 2.2:+30050108 None:intergenic
GAACATCGACCGGAAACTGG+TGG 0.598498 2.2:+30049248 None:intergenic
ATAATGAGTTACAATCTTGG+TGG 0.599058 2.2:-30050060 MS.gene83325:CDS
TAATGAGTTACAATCTTGGT+GGG 0.599305 2.2:-30050059 MS.gene83325:CDS
GTTGCTGTTCTTCGCTACGT+TGG 0.607168 2.2:-30049629 MS.gene83325:CDS
TGCAATTCTACTTTCTAACA+GGG 0.608164 2.2:-30049866 MS.gene83325:CDS
GGTGGATCTCATCGATCCAA+GGG 0.617008 2.2:+30049266 None:intergenic
GTACCAAAGCGAACTGCTAG+AGG 0.619086 2.2:+30049426 None:intergenic
AGATATCATAGATATCATTG+AGG 0.623017 2.2:+30050556 None:intergenic
GCTGCAATTAACACCACAAG+TGG 0.623028 2.2:+30050597 None:intergenic
TGCTTAGAACAGCTTCTGGT+AGG 0.624810 2.2:+30050277 None:intergenic
GGATATTTATGGATCACTCG+AGG 0.626023 2.2:-30050380 MS.gene83325:CDS
CCTTGTTAGTGCGAACGGTG+TGG 0.627883 2.2:+30049809 None:intergenic
TCAAGAAAGGAGATAGAACA+TGG 0.636177 2.2:-30049372 MS.gene83325:CDS
AAACAAACATTACCACGTTG+GGG 0.638025 2.2:-30049678 MS.gene83325:CDS
AGAAGCTAGGAAAGAAGCGG+AGG 0.638732 2.2:-30050232 MS.gene83325:CDS
CTTGACGGCTCCATATGCCA+TGG 0.639071 2.2:+30049475 None:intergenic
TCGGACGTTGGAAGGACAAG+TGG 0.640146 2.2:+30049655 None:intergenic
GACGACGATGGCGCTAACAA+GGG 0.641367 2.2:+30050717 None:intergenic
GATATTTATGGATCACTCGA+GGG 0.648472 2.2:-30050379 MS.gene83325:CDS
TTGGCTTACACGGAAGCCAA+GGG 0.648617 2.2:-30049400 MS.gene83325:CDS
CTTACACGGAAGCCAAGGGA+AGG 0.652154 2.2:-30049396 MS.gene83325:CDS
GAAAGAAGCTAGGAAAGAAG+CGG 0.654182 2.2:-30050235 MS.gene83325:CDS
ATCAATCACTATAAGCTGAG+AGG 0.664913 2.2:-30050087 MS.gene83325:CDS
GCAATTCTACTTTCTAACAG+GGG 0.671034 2.2:-30049865 MS.gene83325:CDS
TGTTAGCGCCATCGTCGTCG+CGG 0.673997 2.2:-30050713 MS.gene83325:CDS
CACACCGTTCGCACTAACAA+GGG 0.677053 2.2:-30049808 MS.gene83325:CDS
ACACAAGAAGAAGACGACAA+TGG 0.692603 2.2:-30049757 MS.gene83325:CDS
GGCGAGTGGTTTCTTCACGG+AGG 0.715615 2.2:-30050358 MS.gene83325:CDS
TGTAACACGAGAAATTAACA+AGG 0.752828 2.2:+30050433 None:intergenic
AAGGACAAGTGGCCCCAACG+TGG 0.761089 2.2:+30049666 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTTATCAAAAGATCTTA+TGG - chr2.2:30050247-30050266 MS.gene83325:CDS 20.0%
! AACATAATGAGTTACAATCT+TGG - chr2.2:30049906-30049925 MS.gene83325:CDS 25.0%
! AGATATCATAGATATCATTG+AGG + chr2.2:30049416-30049435 None:intergenic 25.0%
! CAATGATATCTATGATATCT+GGG - chr2.2:30049416-30049435 MS.gene83325:CDS 25.0%
! GATATCATAGATATCATTGA+GGG + chr2.2:30049415-30049434 None:intergenic 25.0%
! TCAATGATATCTATGATATC+TGG - chr2.2:30049415-30049434 MS.gene83325:CDS 25.0%
!!! TTCACTGATTTTTTGAAATC+TGG + chr2.2:30049288-30049307 None:intergenic 25.0%
AAAACAAACATTACCACGTT+GGG - chr2.2:30050290-30050309 MS.gene83325:CDS 30.0%
AAAAGGTCGATAAAAACACA+AGG + chr2.2:30049488-30049507 None:intergenic 30.0%
AAATTGAAGTATGTGAAGCT+TGG - chr2.2:30049306-30049325 MS.gene83325:CDS 30.0%
ATAATGAGTTACAATCTTGG+TGG - chr2.2:30049909-30049928 MS.gene83325:CDS 30.0%
ATCAGAATTCCAATGTCTTT+AGG + chr2.2:30049813-30049832 None:intergenic 30.0%
ATGCACTAAGAGGATATTTA+TGG - chr2.2:30049578-30049597 MS.gene83325:CDS 30.0%
TAATGAGTTACAATCTTGGT+GGG - chr2.2:30049910-30049929 MS.gene83325:CDS 30.0%
TATCATTACCTAATCACTCA+TGG - chr2.2:30049330-30049349 MS.gene83325:CDS 30.0%
TGCAATTCTACTTTCTAACA+GGG - chr2.2:30050103-30050122 MS.gene83325:CDS 30.0%
TGTAACACGAGAAATTAACA+AGG + chr2.2:30049539-30049558 None:intergenic 30.0%
! AGAATGACACTTTATCGTTT+TGG - chr2.2:30050515-30050534 MS.gene83325:CDS 30.0%
! GAAGATAAAGCCTTATATTC+CGG - chr2.2:30050385-30050404 MS.gene83325:CDS 30.0%
!!! CTGATTTTTTGAAATCTGGA+AGG + chr2.2:30049284-30049303 None:intergenic 30.0%
AAACAAACATTACCACGTTG+GGG - chr2.2:30050291-30050310 MS.gene83325:CDS 35.0%
AGAACATGGCAAATAGCATT+TGG - chr2.2:30050611-30050630 MS.gene83325:CDS 35.0%
AGCTTCTTTCATTGAAGGAT+TGG + chr2.2:30049724-30049743 None:intergenic 35.0%
AGCTTGAAATCCGGAATATA+AGG + chr2.2:30050398-30050417 None:intergenic 35.0%
AGGACGAGTCATAAAGTAAA+AGG + chr2.2:30049505-30049524 None:intergenic 35.0%
ATCAATCACTATAAGCTGAG+AGG - chr2.2:30049882-30049901 MS.gene83325:CDS 35.0%
ATCCTTCAATGAAAGAAGCT+AGG - chr2.2:30049724-30049743 MS.gene83325:CDS 35.0%
CAAACAATTCGAAACAAGCT+TGG + chr2.2:30050066-30050085 None:intergenic 35.0%
CTGCAATTCTACTTTCTAAC+AGG - chr2.2:30050102-30050121 MS.gene83325:CDS 35.0%
GAAAACAAACATTACCACGT+TGG - chr2.2:30050289-30050308 MS.gene83325:CDS 35.0%
GAGAAATTAACAAGGTAGAC+TGG + chr2.2:30049531-30049550 None:intergenic 35.0%
GAGAAGATCCAAATACCAAT+TGG - chr2.2:30050136-30050155 MS.gene83325:CDS 35.0%
GAGAGTCGAGCAAATAATAA+CGG + chr2.2:30049466-30049485 None:intergenic 35.0%
GATATTTATGGATCACTCGA+GGG - chr2.2:30049590-30049609 MS.gene83325:CDS 35.0%
GCAATTCTACTTTCTAACAG+GGG - chr2.2:30050104-30050123 MS.gene83325:CDS 35.0%
GCTTCTTTCATTGAAGGATT+GGG + chr2.2:30049723-30049742 None:intergenic 35.0%
TAATTTCTCGTGTTACAAGC+CGG - chr2.2:30049542-30049561 MS.gene83325:CDS 35.0%
TCAAGAAAGGAGATAGAACA+TGG - chr2.2:30050597-30050616 MS.gene83325:CDS 35.0%
TCGAATTGTTTGTTCCGTAT+GGG - chr2.2:30050074-30050093 MS.gene83325:CDS 35.0%
TTCCTAGCTTCTTTCATTGA+AGG + chr2.2:30049729-30049748 None:intergenic 35.0%
TTCGAATTGTTTGTTCCGTA+TGG - chr2.2:30050073-30050092 MS.gene83325:CDS 35.0%
TTTACTTTATGACTCGTCCT+AGG - chr2.2:30049505-30049524 MS.gene83325:CDS 35.0%
! CGATAAAGTGTCATTCTTGA+CGG + chr2.2:30050512-30050531 None:intergenic 35.0%
! TCAAAAGCTAGCTTGAAATC+CGG + chr2.2:30050407-30050426 None:intergenic 35.0%
!! CGTGGTAATGTTTGTTTTCA+AGG + chr2.2:30050288-30050307 None:intergenic 35.0%
AAAACTTGAAGCTGTCTCCA+TGG - chr2.2:30050477-30050496 MS.gene83325:CDS 40.0%
AATTGTTTGTTCCGTATGGG+AGG - chr2.2:30050077-30050096 MS.gene83325:CDS 40.0%
ACACAAGAAGAAGACGACAA+TGG - chr2.2:30050212-30050231 MS.gene83325:CDS 40.0%
ACGGTAAAGCCTAAAGACAT+TGG - chr2.2:30049801-30049820 MS.gene83325:CDS 40.0%
AGTTACAATCTTGGTGGGAT+GGG - chr2.2:30049915-30049934 MS.gene83325:CDS 40.0%
ATCACGTTGAATTGGTATCC+CGG - chr2.2:30050029-30050048 MS.gene83325:CDS 40.0%
ATCTCTCAAGGATCTTTCGT+TGG + chr2.2:30049650-30049669 None:intergenic 40.0%
ATGATCATTGCAGAGAGTGA+CGG + chr2.2:30049864-30049883 None:intergenic 40.0%
ATTGTTTGTTCCGTATGGGA+GGG - chr2.2:30050078-30050097 MS.gene83325:CDS 40.0%
CTACAAAAGAGAAGAACCCT+TGG - chr2.2:30050687-30050706 MS.gene83325:CDS 40.0%
GAAAGAAGCTAGGAAAGAAG+CGG - chr2.2:30049734-30049753 MS.gene83325:CDS 40.0%
GAAATTCTTGGAACATCGAC+CGG + chr2.2:30050734-30050753 None:intergenic 40.0%
GAACATTTCTGCATTCATGC+CGG - chr2.2:30050425-30050444 MS.gene83325:CDS 40.0%
GGATATTTATGGATCACTCG+AGG - chr2.2:30049589-30049608 MS.gene83325:CDS 40.0%
TCTACTTTCTAACAGGGGAT+CGG - chr2.2:30050109-30050128 MS.gene83325:CDS 40.0%
TCTTTCATTGAAGGATTGGG+AGG + chr2.2:30049720-30049739 None:intergenic 40.0%
TGGAGAACATCACGTTGAAT+TGG - chr2.2:30050021-30050040 MS.gene83325:CDS 40.0%
! ATCTAAAACAGCTCTTCCAC+CGG + chr2.2:30050447-30050466 None:intergenic 40.0%
! CAAGGGTTCTTCTCTTTTGT+AGG + chr2.2:30050689-30050708 None:intergenic 40.0%
! TGGCAAATAGCATTTGGTTC+TGG - chr2.2:30050617-30050636 MS.gene83325:CDS 40.0%
! TTGGTACGAATTGGCTTACA+CGG - chr2.2:30050559-30050578 MS.gene83325:CDS 40.0%
!! TGAGCTGTTTTCGAAGACAA+CGG - chr2.2:30049782-30049801 MS.gene83325:CDS 40.0%
AAAGATCTTATGGCCGTTGC+AGG - chr2.2:30050257-30050276 MS.gene83325:CDS 45.0%
ACAACCAACGCATACGAGTT+AGG + chr2.2:30049996-30050015 None:intergenic 45.0%
AGCGAAGAACAGCAACTGTT+CGG + chr2.2:30050336-30050355 None:intergenic 45.0%
AGTCTCGCAAATGCACTAAG+AGG - chr2.2:30049568-30049587 MS.gene83325:CDS 45.0%
ATCCTTGAGAGATCTGGTCT+TGG - chr2.2:30049657-30049676 MS.gene83325:CDS 45.0%
CAGTTCGCTTTGGTACGAAT+TGG - chr2.2:30050550-30050569 MS.gene83325:CDS 45.0%
CATACGAGTTAGGATGAACC+TGG + chr2.2:30049986-30050005 None:intergenic 45.0%
CCACAAGTGGTGAAAGAAAG+AGG + chr2.2:30049362-30049381 None:intergenic 45.0%
CCTCTTTCTTTCACCACTTG+TGG - chr2.2:30049359-30049378 MS.gene83325:CDS 45.0%
CGAAAGATCCTTGAGAGATC+TGG - chr2.2:30049651-30049670 MS.gene83325:CDS 45.0%
GAAGACGACAATGGCAAAGT+TGG - chr2.2:30050221-30050240 MS.gene83325:CDS 45.0%
GAGTTACAATCTTGGTGGGA+TGG - chr2.2:30049914-30049933 MS.gene83325:CDS 45.0%
GCTGCAATTAACACCACAAG+TGG + chr2.2:30049375-30049394 None:intergenic 45.0%
GGAATGCTTAGAACAGCTTC+TGG + chr2.2:30049699-30049718 None:intergenic 45.0%
GGTACATGCCATGAGTGATT+AGG + chr2.2:30049341-30049360 None:intergenic 45.0%
GGTGTGGACCAATTGGTATT+TGG + chr2.2:30050147-30050166 None:intergenic 45.0%
TCACGTTGAATTGGTATCCC+GGG - chr2.2:30050030-30050049 MS.gene83325:CDS 45.0%
TCATCCTAACTCGTATGCGT+TGG - chr2.2:30049989-30050008 MS.gene83325:CDS 45.0%
TCCTTTCTTGATCCTTCCCT+TGG + chr2.2:30050588-30050607 None:intergenic 45.0%
TCTTGCTAAAGAGCTTCTCC+AGG - chr2.2:30049965-30049984 MS.gene83325:CDS 45.0%
TGCTTAGAACAGCTTCTGGT+AGG + chr2.2:30049695-30049714 None:intergenic 45.0%
TTGTTTGTTCCGTATGGGAG+GGG - chr2.2:30050079-30050098 MS.gene83325:CDS 45.0%
TTTCTAACAGGGGATCGGAT+AGG - chr2.2:30050114-30050133 MS.gene83325:CDS 45.0%
! TTTCAAGGCATCACCTGCAA+CGG + chr2.2:30050273-30050292 None:intergenic 45.0%
!! GTATGCGTTGGTTGTTAGCA+TGG - chr2.2:30050001-30050020 MS.gene83325:CDS 45.0%
!! TTAACAAGGTAGACTGGCCT+AGG + chr2.2:30049525-30049544 None:intergenic 45.0%
AACAGCAACTGTTCGGACGT+TGG + chr2.2:30050329-30050348 None:intergenic 50.0%
AAGCTTGGATCGATCGTTCC+CGG + chr2.2:30050051-30050070 None:intergenic 50.0%
ACACCTCTAGCAGTTCGCTT+TGG - chr2.2:30050540-30050559 MS.gene83325:CDS 50.0%
AGAAGCTAGGAAAGAAGCGG+AGG - chr2.2:30049737-30049756 MS.gene83325:CDS 50.0%
AGCACCCTTGTTAGTGCGAA+CGG + chr2.2:30050168-30050187 None:intergenic 50.0%
CACACCGTTCGCACTAACAA+GGG - chr2.2:30050161-30050180 MS.gene83325:CDS 50.0%
CATTTCTGCATTCATGCCGG+TGG - chr2.2:30050428-30050447 MS.gene83325:CDS 50.0%
CGATGAGATCCACCAGTTTC+CGG - chr2.2:30050712-30050731 MS.gene83325:CDS 50.0%
CTCCAAGACCAGATCTCTCA+AGG + chr2.2:30049662-30049681 None:intergenic 50.0%
CTGTTCTTCGCTACGTTGGT+TGG - chr2.2:30050344-30050363 MS.gene83325:CDS 50.0%
GAAGCGGAGGCAGTTATGTT+TGG - chr2.2:30049750-30049769 MS.gene83325:CDS 50.0%
GAAGCTGTCTCCATGGCATA+TGG - chr2.2:30050484-30050503 MS.gene83325:CDS 50.0%
GCCAAGGGAAGGATCAAGAA+AGG - chr2.2:30050584-30050603 MS.gene83325:CDS 50.0%
GGTGGATCTCATCGATCCAA+GGG + chr2.2:30050706-30050725 None:intergenic 50.0%
GTACCAAAGCGAACTGCTAG+AGG + chr2.2:30050546-30050565 None:intergenic 50.0%
GTGCATTTGCGAGACTCTTC+CGG + chr2.2:30049564-30049583 None:intergenic 50.0%
TCAAGTGCAACAGCGCAGTA+TGG - chr2.2:30050642-30050661 MS.gene83325:CDS 50.0%
TTGGAACATCGACCGGAAAC+TGG + chr2.2:30050727-30050746 None:intergenic 50.0%
TTGGCTTACACGGAAGCCAA+GGG - chr2.2:30050569-30050588 MS.gene83325:CDS 50.0%
! ATTGCAGAGAGTGACGGAGT+TGG + chr2.2:30049858-30049877 None:intergenic 50.0%
! ATTGGCTTACACGGAAGCCA+AGG - chr2.2:30050568-30050587 MS.gene83325:CDS 50.0%
! GTTGCTGTTCTTCGCTACGT+TGG - chr2.2:30050340-30050359 MS.gene83325:CDS 50.0%
! TGGTGGATCTCATCGATCCA+AGG + chr2.2:30050707-30050726 None:intergenic 50.0%
AGCTTGGATCGATCGTTCCC+GGG + chr2.2:30050050-30050069 None:intergenic 55.0%
CCACACCGTTCGCACTAACA+AGG - chr2.2:30050160-30050179 MS.gene83325:CDS 55.0%
CCTTGTTAGTGCGAACGGTG+TGG + chr2.2:30050163-30050182 None:intergenic 55.0%
CTTACACGGAAGCCAAGGGA+AGG - chr2.2:30050573-30050592 MS.gene83325:CDS 55.0%
CTTGACGGCTCCATATGCCA+TGG + chr2.2:30050497-30050516 None:intergenic 55.0%
GAACATCGACCGGAAACTGG+TGG + chr2.2:30050724-30050743 None:intergenic 55.0%
GAATTGCAGCCCCTCCCATA+CGG + chr2.2:30050091-30050110 None:intergenic 55.0%
GACGACGATGGCGCTAACAA+GGG + chr2.2:30049255-30049274 None:intergenic 55.0%
GAGGGCGAGTGGTTTCTTCA+CGG - chr2.2:30049608-30049627 MS.gene83325:CDS 55.0%
GCAACTGTTCGGACGTTGGA+AGG + chr2.2:30050325-30050344 None:intergenic 55.0%
GCGCAGTATGGAAGGCTCTA+AGG - chr2.2:30050654-30050673 MS.gene83325:CDS 55.0%
GTGCAACAGCGCAGTATGGA+AGG - chr2.2:30050646-30050665 MS.gene83325:CDS 55.0%
TCGGACGTTGGAAGGACAAG+TGG + chr2.2:30050317-30050336 None:intergenic 55.0%
TGCGAACGGTGTGGACCAAT+TGG + chr2.2:30050154-30050173 None:intergenic 55.0%
AAGGACAAGTGGCCCCAACG+TGG + chr2.2:30050306-30050325 None:intergenic 60.0%
ATGGATCACTCGAGGGCGAG+TGG - chr2.2:30049597-30049616 MS.gene83325:CDS 60.0%
CGACGACGATGGCGCTAACA+AGG + chr2.2:30049256-30049275 None:intergenic 60.0%
GGCGAGTGGTTTCTTCACGG+AGG - chr2.2:30049611-30049630 MS.gene83325:CDS 60.0%
GTGGGATGGGATGCAGTGCT+GGG - chr2.2:30049928-30049947 MS.gene83325:CDS 60.0%
TGTTAGCGCCATCGTCGTCG+CGG - chr2.2:30049256-30049275 MS.gene83325:CDS 60.0%
GGAAGGATCCGCGACGACGA+TGG + chr2.2:30049267-30049286 None:intergenic 65.0%
GGTGGGATGGGATGCAGTGC+TGG - chr2.2:30049927-30049946 MS.gene83325:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 30049228 30050763 30049228 ID=MS.gene83325
chr2.2 mRNA 30049228 30050763 30049228 ID=MS.gene83325.t1;Parent=MS.gene83325
chr2.2 exon 30049228 30050763 30049228 ID=MS.gene83325.t1.exon1;Parent=MS.gene83325.t1
chr2.2 CDS 30049228 30050763 30049228 ID=cds.MS.gene83325.t1;Parent=MS.gene83325.t1
Gene Sequence

>MS.gene83325

ATGACAGAATCAAAACAAGATACACCCTTGTTAGCGCCATCGTCGTCGCGGATCCTTCCAGATTTCAAAAAATCAGTGAAATTGAAGTATGTGAAGCTTGGTTATCATTACCTAATCACTCATGGCATGTACCTCTTTCTTTCACCACTTGTGGTGTTAATTGCAGCTCAGTTATCAACTTTCTCCCTCAATGATATCTATGATATCTGGGAAAATCTGCAGTACAATCTCGTATCCGTTATTATTTGCTCGACTCTCCTTGTGTTTTTATCGACCTTTTACTTTATGACTCGTCCTAGGCCAGTCTACCTTGTTAATTTCTCGTGTTACAAGCCGGAAGAGTCTCGCAAATGCACTAAGAGGATATTTATGGATCACTCGAGGGCGAGTGGTTTCTTCACGGAGGAGAATCTTGATTTCCAACGAAAGATCCTTGAGAGATCTGGTCTTGGAGAAAACACTTACCTACCAGAAGCTGTTCTAAGCATTCCTCCCAATCCTTCAATGAAAGAAGCTAGGAAAGAAGCGGAGGCAGTTATGTTTGGTGCTATCGATGAGCTGTTTTCGAAGACAACGGTAAAGCCTAAAGACATTGGAATTCTGATTGTGAATTGTAGTCTTTTCTGTCCAACTCCGTCACTCTCTGCAATGATCATCAATCACTATAAGCTGAGAGGTAACATAATGAGTTACAATCTTGGTGGGATGGGATGCAGTGCTGGGATTATTTCAATTGATCTTGCTAAAGAGCTTCTCCAGGTTCATCCTAACTCGTATGCGTTGGTTGTTAGCATGGAGAACATCACGTTGAATTGGTATCCCGGGAACGATCGATCCAAGCTTGTTTCGAATTGTTTGTTCCGTATGGGAGGGGCTGCAATTCTACTTTCTAACAGGGGATCGGATAGGAGAAGATCCAAATACCAATTGGTCCACACCGTTCGCACTAACAAGGGTGCTGATGACAAGTGCTTCAGCTGCGTTACACAAGAAGAAGACGACAATGGCAAAGTTGGTGTTACTTTATCAAAAGATCTTATGGCCGTTGCAGGTGATGCCTTGAAAACAAACATTACCACGTTGGGGCCACTTGTCCTTCCAACGTCCGAACAGTTGCTGTTCTTCGCTACGTTGGTTGGAAAGAAACTCTTCAAGATGAAGATAAAGCCTTATATTCCGGATTTCAAGCTAGCTTTTGAACATTTCTGCATTCATGCCGGTGGAAGAGCTGTTTTAGATGAGCTAGAGAAAAACTTGAAGCTGTCTCCATGGCATATGGAGCCGTCAAGAATGACACTTTATCGTTTTGGAAACACCTCTAGCAGTTCGCTTTGGTACGAATTGGCTTACACGGAAGCCAAGGGAAGGATCAAGAAAGGAGATAGAACATGGCAAATAGCATTTGGTTCTGGATTCAAGTGCAACAGCGCAGTATGGAAGGCTCTAAGGACAATCAATCCTACAAAAGAGAAGAACCCTTGGATCGATGAGATCCACCAGTTTCCGGTCGATGTTCCAAGAATTTCTGCCATCTAA
Protein sequence

>MS.gene83325.t1

MTESKQDTPLLAPSSSRILPDFKKSVKLKYVKLGYHYLITHGMYLFLSPLVVLIAAQLSTFSLNDIYDIWENLQYNLVSVIICSTLLVFLSTFYFMTRPRPVYLVNFSCYKPEESRKCTKRIFMDHSRASGFFTEENLDFQRKILERSGLGENTYLPEAVLSIPPNPSMKEARKEAEAVMFGAIDELFSKTTVKPKDIGILIVNCSLFCPTPSLSAMIINHYKLRGNIMSYNLGGMGCSAGIISIDLAKELLQVHPNSYALVVSMENITLNWYPGNDRSKLVSNCLFRMGGAAILLSNRGSDRRRSKYQLVHTVRTNKGADDKCFSCVTQEEDDNGKVGVTLSKDLMAVAGDALKTNITTLGPLVLPTSEQLLFFATLVGKKLFKMKIKPYIPDFKLAFEHFCIHAGGRAVLDELEKNLKLSPWHMEPSRMTLYRFGNTSSSSLWYELAYTEAKGRIKKGDRTWQIAFGSGFKCNSAVWKALRTINPTKEKNPWIDEIHQFPVDVPRISAI