Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83430.t1 | XP_013449506.1 | 99.2 | 124 | 1 | 0 | 4 | 127 | 82 | 205 | 4.60E-63 | 250.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83430.t1 | A0A072U1E7 | 99.2 | 124 | 1 | 0 | 4 | 127 | 82 | 205 | 3.3e-63 | 250.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051147 | MS.gene83430 | 0.802421 | 5.65E-49 | -1.69E-46 |
| MS.gene052674 | MS.gene83430 | 0.800683 | 1.28E-48 | -1.69E-46 |
| MS.gene054479 | MS.gene83430 | 0.810971 | 8.84E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene83430 | 0.807625 | 4.61E-50 | -1.69E-46 |
| MS.gene058081 | MS.gene83430 | 0.804484 | 2.11E-49 | -1.69E-46 |
| MS.gene059283 | MS.gene83430 | 0.817468 | 3.25E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene83430 | 0.838267 | 3.18E-57 | -1.69E-46 |
| MS.gene060802 | MS.gene83430 | 0.802046 | 6.76E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene83430 | 0.809756 | 1.62E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGCTTCTTTGGATGATTT+TGG | 0.165257 | 7.2:-22114521 | None:intergenic |
| AACTTACCAATAAGCTTCTT+TGG | 0.237998 | 7.2:-22114531 | None:intergenic |
| AATCATCCAAAGAAGCTTAT+TGG | 0.258024 | 7.2:+22114525 | MS.gene83430:CDS |
| AGGAAGCTTACAACCTTTCA+TGG | 0.340391 | 7.2:-22114479 | None:intergenic |
| GTCAAGAGAGATATGAAGTT+AGG | 0.367836 | 7.2:+22114714 | MS.gene83430:CDS |
| CCCCATAGCCTTCATCCTCT+TGG | 0.402169 | 7.2:-22114865 | None:intergenic |
| TCAAGAGAGATATGAAGTTA+GGG | 0.417350 | 7.2:+22114715 | MS.gene83430:CDS |
| GGCCGGAGATAACAGAAAAT+CGG | 0.460576 | 7.2:+22114297 | MS.gene83430:CDS |
| AACATCGCAAGTGCCTTTAC+CGG | 0.498467 | 7.2:-22114374 | None:intergenic |
| TCAGCAGAGGACACCGGTAA+AGG | 0.516208 | 7.2:+22114361 | MS.gene83430:CDS |
| GGAGAAGAAAAGTAACAAGA+AGG | 0.520874 | 7.2:+22114736 | MS.gene83430:CDS |
| GAGAGAGTAATGTAAAGCTT+AGG | 0.521687 | 7.2:-22114427 | None:intergenic |
| GGCACTTGCGATGTTATCAA+TGG | 0.530662 | 7.2:+22114382 | MS.gene83430:CDS |
| TAACAGAAAATCGGCATCAC+CGG | 0.532579 | 7.2:+22114306 | MS.gene83430:CDS |
| ATATCAGTCAACCATGCTCC+AGG | 0.532665 | 7.2:-22114689 | None:intergenic |
| AAAATCGGCATCACCGGAGA+AGG | 0.542456 | 7.2:+22114312 | MS.gene83430:CDS |
| TTGGTATGCAGGAATTCCGA+TGG | 0.546689 | 7.2:+22114256 | MS.gene83430:CDS |
| TAGTATCGATCTTCCTTCTC+CGG | 0.549815 | 7.2:-22114325 | None:intergenic |
| ACTATTCTACGACCGGCGGC+CGG | 0.561495 | 7.2:+22114280 | MS.gene83430:CDS |
| CGATGGAACTATTCTACGAC+CGG | 0.568292 | 7.2:+22114273 | MS.gene83430:CDS |
| AGTTGGTAAGTCCTGGAGCA+TGG | 0.569217 | 7.2:+22114678 | MS.gene83430:intron |
| TTTCTGTTATCTCCGGCCGC+CGG | 0.574206 | 7.2:-22114292 | None:intergenic |
| TGCAGAGGCCAAGAGGATGA+AGG | 0.592255 | 7.2:+22114857 | MS.gene83430:intron |
| CGGTCGTAGAATAGTTCCAT+CGG | 0.626224 | 7.2:-22114272 | None:intergenic |
| AGATCGATACTACACGACGA+GGG | 0.631631 | 7.2:+22114336 | MS.gene83430:CDS |
| GATCGATACTACACGACGAG+GGG | 0.647579 | 7.2:+22114337 | MS.gene83430:CDS |
| ATCGATACTACACGACGAGG+GGG | 0.671513 | 7.2:+22114338 | MS.gene83430:CDS |
| TGGAACTATTCTACGACCGG+CGG | 0.694045 | 7.2:+22114276 | MS.gene83430:CDS |
| AAGATCGATACTACACGACG+AGG | 0.699452 | 7.2:+22114335 | MS.gene83430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAGAGATAAAATAAAAA+AGG | - | chr7.2:22114801-22114820 | None:intergenic | 10.0% |
| !!! | TTTTTATGTTGTTTTTATTA+TGG | + | chr7.2:22114576-22114595 | MS.gene83430:intron | 10.0% |
| !!! | TGTTTTTATTATGGTTAATT+GGG | + | chr7.2:22114585-22114604 | MS.gene83430:intron | 15.0% |
| !!! | TTGTTTTTATTATGGTTAAT+TGG | + | chr7.2:22114584-22114603 | MS.gene83430:intron | 15.0% |
| ! | TTATTATGGTTAATTGGGTT+TGG | + | chr7.2:22114590-22114609 | MS.gene83430:intron | 25.0% |
| AAAAGTAACAAGAAGGTAAG+TGG | + | chr7.2:22114743-22114762 | MS.gene83430:intron | 30.0% | |
| AACTTACCAATAAGCTTCTT+TGG | - | chr7.2:22114534-22114553 | None:intergenic | 30.0% | |
| AATCATCCAAAGAAGCTTAT+TGG | + | chr7.2:22114525-22114544 | MS.gene83430:CDS | 30.0% | |
| TCAAGAGAGATATGAAGTTA+GGG | + | chr7.2:22114715-22114734 | MS.gene83430:CDS | 30.0% | |
| ! | TAAGCTTCTTTGGATGATTT+TGG | - | chr7.2:22114524-22114543 | None:intergenic | 30.0% |
| !!! | TTTGGTGTTTGTTTTACAGT+TGG | + | chr7.2:22114661-22114680 | MS.gene83430:intron | 30.0% |
| ACAAACACCAAACAAACATG+TGG | - | chr7.2:22114653-22114672 | None:intergenic | 35.0% | |
| ATGTTGTCCACATGTTTGTT+TGG | + | chr7.2:22114643-22114662 | MS.gene83430:intron | 35.0% | |
| GAGAAGAAAAAGCTCTTGTT+TGG | + | chr7.2:22114404-22114423 | MS.gene83430:CDS | 35.0% | |
| GAGAGAGTAATGTAAAGCTT+AGG | - | chr7.2:22114430-22114449 | None:intergenic | 35.0% | |
| GGAGAAGAAAAGTAACAAGA+AGG | + | chr7.2:22114736-22114755 | MS.gene83430:CDS | 35.0% | |
| GTCAAGAGAGATATGAAGTT+AGG | + | chr7.2:22114714-22114733 | MS.gene83430:CDS | 35.0% | |
| ! | GAAGATTTTCTTGCCATGAA+AGG | + | chr7.2:22114466-22114485 | MS.gene83430:CDS | 35.0% |
| !! | ATGATTTTGGCCCTCTTTTT+GGG | - | chr7.2:22114511-22114530 | None:intergenic | 35.0% |
| !!! | TTTTTGGGTTTTTGATGCAG+AGG | + | chr7.2:22114842-22114861 | MS.gene83430:intron | 35.0% |
| ACAAGAAGGTAAGTGGTTGT+TGG | + | chr7.2:22114750-22114769 | MS.gene83430:intron | 40.0% | |
| AGGAAGCTTACAACCTTTCA+TGG | - | chr7.2:22114482-22114501 | None:intergenic | 40.0% | |
| TAACAGAAAATCGGCATCAC+CGG | + | chr7.2:22114306-22114325 | MS.gene83430:CDS | 40.0% | |
| TCCTCAAAGACCCAAAAAGA+GGG | + | chr7.2:22114498-22114517 | MS.gene83430:CDS | 40.0% | |
| TTCCTCAAAGACCCAAAAAG+AGG | + | chr7.2:22114497-22114516 | MS.gene83430:CDS | 40.0% | |
| !! | GATGATTTTGGCCCTCTTTT+TGG | - | chr7.2:22114512-22114531 | None:intergenic | 40.0% |
| !! | TAGTATCGATCTTCCTTCTC+CGG | - | chr7.2:22114328-22114347 | None:intergenic | 40.0% |
| !!! | GTTTTACAGTTGGTAAGTCC+TGG | + | chr7.2:22114671-22114690 | MS.gene83430:intron | 40.0% |
| !!! | TGCCGATTTTCTGTTATCTC+CGG | - | chr7.2:22114302-22114321 | None:intergenic | 40.0% |
| AACATCGCAAGTGCCTTTAC+CGG | - | chr7.2:22114377-22114396 | None:intergenic | 45.0% | |
| AAGATCGATACTACACGACG+AGG | + | chr7.2:22114335-22114354 | MS.gene83430:CDS | 45.0% | |
| AGATCGATACTACACGACGA+GGG | + | chr7.2:22114336-22114355 | MS.gene83430:CDS | 45.0% | |
| ATATCAGTCAACCATGCTCC+AGG | - | chr7.2:22114692-22114711 | None:intergenic | 45.0% | |
| CGATGGAACTATTCTACGAC+CGG | + | chr7.2:22114273-22114292 | MS.gene83430:CDS | 45.0% | |
| CGGTCGTAGAATAGTTCCAT+CGG | - | chr7.2:22114275-22114294 | None:intergenic | 45.0% | |
| GGCACTTGCGATGTTATCAA+TGG | + | chr7.2:22114382-22114401 | MS.gene83430:CDS | 45.0% | |
| GGCCGGAGATAACAGAAAAT+CGG | + | chr7.2:22114297-22114316 | MS.gene83430:CDS | 45.0% | |
| TTGGTATGCAGGAATTCCGA+TGG | + | chr7.2:22114256-22114275 | MS.gene83430:CDS | 45.0% | |
| ! | TTTTTGATGCAGAGGCCAAG+AGG | + | chr7.2:22114850-22114869 | MS.gene83430:intron | 45.0% |
| !!! | TTTTTCTAAATATAATTTTT+GGG | + | chr7.2:22114827-22114846 | MS.gene83430:intron | 5.0% |
| !!! | TTTTTTCTAAATATAATTTT+TGG | + | chr7.2:22114826-22114845 | MS.gene83430:intron | 5.0% |
| AAAATCGGCATCACCGGAGA+AGG | + | chr7.2:22114312-22114331 | MS.gene83430:CDS | 50.0% | |
| ATCGATACTACACGACGAGG+GGG | + | chr7.2:22114338-22114357 | MS.gene83430:CDS | 50.0% | |
| GATCGATACTACACGACGAG+GGG | + | chr7.2:22114337-22114356 | MS.gene83430:CDS | 50.0% | |
| TGGAACTATTCTACGACCGG+CGG | + | chr7.2:22114276-22114295 | MS.gene83430:CDS | 50.0% | |
| !! | AGTTGGTAAGTCCTGGAGCA+TGG | + | chr7.2:22114678-22114697 | MS.gene83430:intron | 50.0% |
| !!! | GCCCTCTTTTTGGGTCTTTG+AGG | - | chr7.2:22114502-22114521 | None:intergenic | 50.0% |
| TCAGCAGAGGACACCGGTAA+AGG | + | chr7.2:22114361-22114380 | MS.gene83430:CDS | 55.0% | |
| TTTCTGTTATCTCCGGCCGC+CGG | - | chr7.2:22114295-22114314 | None:intergenic | 55.0% | |
| ACTATTCTACGACCGGCGGC+CGG | + | chr7.2:22114280-22114299 | MS.gene83430:CDS | 60.0% | |
| AGGGGGTCAGCAGAGGACAC+CGG | + | chr7.2:22114355-22114374 | MS.gene83430:CDS | 65.0% | |
| CACGACGAGGGGGTCAGCAG+AGG | + | chr7.2:22114348-22114367 | MS.gene83430:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 22114254 | 22114876 | 22114254 | ID=MS.gene83430 |
| chr7.2 | mRNA | 22114254 | 22114876 | 22114254 | ID=MS.gene83430.t1;Parent=MS.gene83430 |
| chr7.2 | exon | 22114254 | 22114546 | 22114254 | ID=MS.gene83430.t1.exon1;Parent=MS.gene83430.t1 |
| chr7.2 | CDS | 22114254 | 22114546 | 22114254 | ID=cds.MS.gene83430.t1;Parent=MS.gene83430.t1 |
| chr7.2 | exon | 22114680 | 22114757 | 22114680 | ID=MS.gene83430.t1.exon2;Parent=MS.gene83430.t1 |
| chr7.2 | CDS | 22114680 | 22114757 | 22114680 | ID=cds.MS.gene83430.t1;Parent=MS.gene83430.t1 |
| chr7.2 | exon | 22114862 | 22114876 | 22114862 | ID=MS.gene83430.t1.exon3;Parent=MS.gene83430.t1 |
| chr7.2 | CDS | 22114862 | 22114876 | 22114862 | ID=cds.MS.gene83430.t1;Parent=MS.gene83430.t1 |
| Gene Sequence |
| Protein sequence |
