Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84137.t1 | XP_024638328.1 | 98.5 | 66 | 1 | 0 | 1 | 66 | 260 | 325 | 6.70E-26 | 125.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84137.t1 | A0A396GQG1 | 95.5 | 66 | 3 | 0 | 1 | 66 | 210 | 275 | 2.0e-24 | 120.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051594 | MS.gene84137 | 0.816155 | 6.40E-52 | -1.69E-46 |
| MS.gene052447 | MS.gene84137 | 0.813144 | 2.97E-51 | -1.69E-46 |
| MS.gene05415 | MS.gene84137 | 0.810477 | 1.13E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene84137 | 0.830633 | 2.63E-55 | -1.69E-46 |
| MS.gene059584 | MS.gene84137 | 0.805646 | 1.21E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84137.t1 | MTR_8g085310 | 54.412 | 68 | 19 | 2 | 1 | 66 | 632 | 689 | 2.86e-14 | 65.5 |
| MS.gene84137.t1 | MTR_4g092300 | 67.347 | 49 | 15 | 1 | 19 | 66 | 759 | 807 | 4.54e-14 | 65.1 |
| MS.gene84137.t1 | MTR_4g051480 | 67.347 | 49 | 15 | 1 | 19 | 66 | 777 | 825 | 5.00e-14 | 64.7 |
| MS.gene84137.t1 | MTR_3g053640 | 70.732 | 41 | 12 | 0 | 26 | 66 | 373 | 413 | 6.93e-12 | 58.5 |
| MS.gene84137.t1 | MTR_1g094760 | 73.810 | 42 | 10 | 1 | 26 | 66 | 641 | 682 | 1.02e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCGTTGGAAAAGTTTA+AGG | 0.170316 | 3.1:-72867243 | MS.gene84137:CDS |
| AGCATGGGCTCAAGTTTCTT+TGG | 0.334501 | 3.1:-72867393 | MS.gene84137:CDS |
| AGTTTAAGGATTCTGATTAG+AGG | 0.439598 | 3.1:-72867229 | None:intergenic |
| GACATTTCAGATGATTCCTA+TGG | 0.446146 | 3.1:-72867266 | MS.gene84137:CDS |
| GGCTACTTCGCGTGTAACTT+CGG | 0.458752 | 3.1:-72867333 | MS.gene84137:CDS |
| CTGATAGATTAAGAAGGGAT+AGG | 0.481174 | 3.1:-72867364 | MS.gene84137:CDS |
| GAATGAGGCACTTGCAGCAT+GGG | 0.501641 | 3.1:-72867408 | MS.gene84137:CDS |
| TAAGAGCTGATAGATTAAGA+AGG | 0.523654 | 3.1:-72867370 | MS.gene84137:CDS |
| AAGAGCTGATAGATTAAGAA+GGG | 0.537119 | 3.1:-72867369 | MS.gene84137:CDS |
| AAGTGTGTGGCTGCTCTCGA+TGG | 0.564826 | 3.1:-72867296 | MS.gene84137:CDS |
| ATCGAGAGCAGCCACACACT+TGG | 0.570899 | 3.1:+72867298 | None:intergenic |
| TGATTCCTATGGAAAAGCGT+TGG | 0.584496 | 3.1:-72867255 | MS.gene84137:CDS |
| TACATCACATCATATGAATG+AGG | 0.591772 | 3.1:-72867423 | None:intergenic |
| TGAATGAGGCACTTGCAGCA+TGG | 0.593350 | 3.1:-72867409 | MS.gene84137:CDS |
| TTGTTCTCTTACCAAGTGTG+TGG | 0.630626 | 3.1:-72867309 | MS.gene84137:CDS |
| AAGAAGGGATAGGAGTGCAG+AGG | 0.647414 | 3.1:-72867354 | MS.gene84137:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAGCTGATAGATTAAGAA+GGG | - | chr3.1:72867273-72867292 | MS.gene84137:CDS | 30.0% | |
| TAAGAGCTGATAGATTAAGA+AGG | - | chr3.1:72867272-72867291 | MS.gene84137:CDS | 30.0% | |
| !! | AAAAGCGTTGGAAAAGTTTA+AGG | - | chr3.1:72867399-72867418 | MS.gene84137:CDS | 30.0% |
| CTGATAGATTAAGAAGGGAT+AGG | - | chr3.1:72867278-72867297 | MS.gene84137:CDS | 35.0% | |
| GACATTTCAGATGATTCCTA+TGG | - | chr3.1:72867376-72867395 | MS.gene84137:CDS | 35.0% | |
| TTGTTCTCTTACCAAGTGTG+TGG | - | chr3.1:72867333-72867352 | MS.gene84137:CDS | 40.0% | |
| ! | CTTTTCCAACGCTTTTCCAT+AGG | + | chr3.1:72867395-72867414 | None:intergenic | 40.0% |
| ! | TGATTCCTATGGAAAAGCGT+TGG | - | chr3.1:72867387-72867406 | MS.gene84137:CDS | 40.0% |
| AGCATGGGCTCAAGTTTCTT+TGG | - | chr3.1:72867249-72867268 | MS.gene84137:CDS | 45.0% | |
| GGCTACTTCGCGTGTAACTT+CGG | - | chr3.1:72867309-72867328 | MS.gene84137:CDS | 50.0% | |
| ! | AAGAAGGGATAGGAGTGCAG+AGG | - | chr3.1:72867288-72867307 | MS.gene84137:CDS | 50.0% |
| ! | GAATGAGGCACTTGCAGCAT+GGG | - | chr3.1:72867234-72867253 | MS.gene84137:CDS | 50.0% |
| ! | TGAATGAGGCACTTGCAGCA+TGG | - | chr3.1:72867233-72867252 | MS.gene84137:CDS | 50.0% |
| AAGTGTGTGGCTGCTCTCGA+TGG | - | chr3.1:72867346-72867365 | MS.gene84137:CDS | 55.0% | |
| ATCGAGAGCAGCCACACACT+TGG | + | chr3.1:72867347-72867366 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 72867232 | 72867432 | 72867232 | ID=MS.gene84137 |
| chr3.1 | mRNA | 72867232 | 72867432 | 72867232 | ID=MS.gene84137.t1;Parent=MS.gene84137 |
| chr3.1 | exon | 72867232 | 72867432 | 72867232 | ID=MS.gene84137.t1.exon1;Parent=MS.gene84137.t1 |
| chr3.1 | CDS | 72867232 | 72867432 | 72867232 | ID=cds.MS.gene84137.t1;Parent=MS.gene84137.t1 |
| Gene Sequence |
| Protein sequence |
