Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05531.t1 | RHN52895.1 | 90 | 50 | 3 | 1 | 2 | 51 | 59 | 106 | 7.60E-11 | 76.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05531.t1 | A0A072UBD1 | 90.0 | 50 | 3 | 1 | 2 | 51 | 27 | 74 | 5.5e-11 | 76.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene05531 | 0.825943 | 3.56E-54 | -1.69E-46 |
| MS.gene053881 | MS.gene05531 | 0.808816 | 2.57E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene05531 | 0.809575 | 1.77E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene059584 | 0.822805 | 1.95E-53 | -1.69E-46 |
| MS.gene05531 | MS.gene059625 | 0.801339 | 9.43E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene06269 | 0.802797 | 4.73E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene062815 | 0.803896 | 2.80E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene063846 | 0.81588 | 7.37E-52 | -1.69E-46 |
| MS.gene05531 | MS.gene064321 | 0.807434 | 5.06E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene064323 | 0.810166 | 1.32E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene064614 | 0.800011 | 1.76E-48 | -1.69E-46 |
| MS.gene05531 | MS.gene064718 | 0.81425 | 1.70E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene065039 | 0.800541 | 1.37E-48 | -1.69E-46 |
| MS.gene05531 | MS.gene065770 | 0.803212 | 3.88E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene066313 | 0.809801 | 1.58E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene066335 | 0.814371 | 1.59E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene066752 | 0.809397 | 1.93E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene068992 | 0.812694 | 3.73E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene069275 | 0.814027 | 1.90E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene071058 | 0.810296 | 1.24E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene071442 | 0.806479 | 8.06E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene071444 | 0.804175 | 2.45E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene09466 | 0.810877 | 9.26E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene09504 | 0.811737 | 6.03E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene24211 | 0.804511 | 2.09E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene26647 | 0.814157 | 1.78E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene29008 | 0.808114 | 3.63E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene29009 | 0.824744 | 6.84E-54 | -1.69E-46 |
| MS.gene05531 | MS.gene29850 | 0.805436 | 1.34E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene30139 | 0.802784 | 4.76E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene34251 | 0.818824 | 1.60E-52 | -1.69E-46 |
| MS.gene05531 | MS.gene34615 | 0.823226 | 1.55E-53 | -1.69E-46 |
| MS.gene05531 | MS.gene38850 | 0.812867 | 3.42E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene41990 | 0.802713 | 4.92E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene42645 | 0.800994 | 1.11E-48 | -1.69E-46 |
| MS.gene05531 | MS.gene44576 | 0.816378 | 5.70E-52 | -1.69E-46 |
| MS.gene05531 | MS.gene45794 | 0.805068 | 1.60E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene47552 | 0.825665 | 4.14E-54 | -1.69E-46 |
| MS.gene05531 | MS.gene47903 | 0.812648 | 3.81E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene49642 | 0.819718 | 1.00E-52 | -1.69E-46 |
| MS.gene05531 | MS.gene49643 | 0.813009 | 3.18E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene50875 | 0.826921 | 2.08E-54 | -1.69E-46 |
| MS.gene05531 | MS.gene51189 | 0.80738 | 5.20E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene51252 | 0.820123 | 8.10E-53 | -1.69E-46 |
| MS.gene05531 | MS.gene54195 | 0.826146 | 3.18E-54 | -1.69E-46 |
| MS.gene05531 | MS.gene54507 | 0.800652 | 1.30E-48 | -1.69E-46 |
| MS.gene05531 | MS.gene54842 | 0.816212 | 6.21E-52 | -1.69E-46 |
| MS.gene05531 | MS.gene54921 | 0.833073 | 6.58E-56 | -1.69E-46 |
| MS.gene05531 | MS.gene61387 | 0.844988 | 5.37E-59 | -1.69E-46 |
| MS.gene05531 | MS.gene61551 | 0.817007 | 4.12E-52 | -1.69E-46 |
| MS.gene05531 | MS.gene62738 | 0.80195 | 7.07E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene63897 | 0.813052 | 3.11E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene65003 | 0.801986 | 6.95E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene66682 | 0.804431 | 2.17E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene68543 | 0.80672 | 7.17E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene73540 | 0.807012 | 6.22E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene76153 | 0.822699 | 2.06E-53 | -1.69E-46 |
| MS.gene05531 | MS.gene76755 | 0.80231 | 5.96E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene76954 | 0.801461 | 8.91E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene78168 | 0.803605 | 3.22E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene80427 | 0.820801 | 5.66E-53 | -1.69E-46 |
| MS.gene05531 | MS.gene81047 | 0.809689 | 1.67E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene81366 | 0.802446 | 5.59E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene82663 | 0.823556 | 1.30E-53 | -1.69E-46 |
| MS.gene05531 | MS.gene82669 | 0.825087 | 5.67E-54 | -1.69E-46 |
| MS.gene05531 | MS.gene82682 | 0.818103 | 2.33E-52 | -1.69E-46 |
| MS.gene05531 | MS.gene82793 | 0.806357 | 8.56E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene82962 | 0.812829 | 3.48E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene84137 | 0.830633 | 2.63E-55 | -1.69E-46 |
| MS.gene05531 | MS.gene88533 | 0.800871 | 1.18E-48 | -1.69E-46 |
| MS.gene05531 | MS.gene88539 | 0.806912 | 6.53E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene89666 | 0.803527 | 3.34E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene91008 | 0.822406 | 2.41E-53 | -1.69E-46 |
| MS.gene05531 | MS.gene91451 | 0.822425 | 2.38E-53 | -1.69E-46 |
| MS.gene05531 | MS.gene92642 | 0.807407 | 5.13E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene93902 | 0.804037 | 2.62E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene96613 | 0.802951 | 4.40E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene96684 | 0.804764 | 1.85E-49 | -1.69E-46 |
| MS.gene05531 | MS.gene97534 | 0.836089 | 1.15E-56 | -1.69E-46 |
| MS.gene05531 | MS.gene98550 | 0.809078 | 2.26E-50 | -1.69E-46 |
| MS.gene05531 | MS.gene99849 | 0.804202 | 2.42E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05531.t1 | MTR_6g086760 | 90.769 | 65 | 4 | 1 | 2 | 66 | 27 | 89 | 1.62e-32 | 108 |
| MS.gene05531.t1 | MTR_6g086765 | 84.314 | 51 | 6 | 1 | 2 | 52 | 44 | 92 | 2.42e-18 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCATTCTCCTGCTGGTT+TGG | 0.217472 | 6.2:+84768617 | None:intergenic |
| TTTATTTGAACTTCGGCTTT+TGG | 0.224067 | 6.2:+84768590 | None:intergenic |
| TTAGATGTACCTTCTTCATT+TGG | 0.332645 | 6.2:+84768653 | None:intergenic |
| GATTTCTTTCATTCTCCTGC+TGG | 0.344587 | 6.2:+84768612 | None:intergenic |
| AAAAGCCGAAGTTCAAATAA+AGG | 0.384311 | 6.2:-84768588 | MS.gene05531:CDS |
| AAATGAAGAAGGTACATCTA+AGG | 0.414920 | 6.2:-84768651 | MS.gene05531:CDS |
| AACATCCTTTATTTGAACTT+CGG | 0.464451 | 6.2:+84768583 | None:intergenic |
| GAATGCAATGCGGACTGCCC+TGG | 0.486235 | 6.2:-84768801 | None:intergenic |
| AGCTGAAGTTCCAAACCAGC+AGG | 0.526615 | 6.2:-84768627 | MS.gene05531:CDS |
| GTTCAAATAAAGGATGTTGA+AGG | 0.541311 | 6.2:-84768578 | MS.gene05531:CDS |
| GCTGAAGTTCCAAATGAAGA+AGG | 0.564225 | 6.2:-84768662 | MS.gene05531:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATCCTTTATTTGAACTT+CGG | + | chr6.2:84768782-84768801 | None:intergenic | 25.0% |
| !!! | AGAAAGAAGCTGATTTTAAA+AGG | - | chr6.2:84768599-84768618 | MS.gene05531:CDS | 25.0% |
| !!! | GAAAGAAGCTGATTTTAAAA+GGG | - | chr6.2:84768600-84768619 | MS.gene05531:CDS | 25.0% |
| AAAAGCCGAAGTTCAAATAA+AGG | - | chr6.2:84768774-84768793 | MS.gene05531:CDS | 30.0% | |
| GTTCAAATAAAGGATGTTGA+AGG | - | chr6.2:84768784-84768803 | MS.gene05531:CDS | 30.0% | |
| TTAGATGTACCTTCTTCATT+TGG | + | chr6.2:84768712-84768731 | None:intergenic | 30.0% | |
| ! | AAATGAAGAAGGTACATCTA+AGG | - | chr6.2:84768711-84768730 | MS.gene05531:CDS | 30.0% |
| ! | TTTATTTGAACTTCGGCTTT+TGG | + | chr6.2:84768775-84768794 | None:intergenic | 30.0% |
| AGATGAAAAAGCTGCATCTA+AGG | - | chr6.2:84768669-84768688 | MS.gene05531:CDS | 35.0% | |
| !! | CTGCTTTTCAATTTCATCCA+GGG | + | chr6.2:84768581-84768600 | None:intergenic | 35.0% |
| !! | TCTGCTTTTCAATTTCATCC+AGG | + | chr6.2:84768582-84768601 | None:intergenic | 35.0% |
| GATTTCTTTCATTCTCCTGC+TGG | + | chr6.2:84768753-84768772 | None:intergenic | 40.0% | |
| GCTGAAGTTCCAAATGAAGA+AGG | - | chr6.2:84768700-84768719 | MS.gene05531:CDS | 40.0% | |
| ! | CTTTCATTCTCCTGCTGGTT+TGG | + | chr6.2:84768748-84768767 | None:intergenic | 45.0% |
| AGCTGAAGTTCCAAACCAGC+AGG | - | chr6.2:84768735-84768754 | MS.gene05531:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 84768568 | 84768816 | 84768568 | ID=MS.gene05531 |
| chr6.2 | mRNA | 84768568 | 84768816 | 84768568 | ID=MS.gene05531.t1;Parent=MS.gene05531 |
| chr6.2 | exon | 84768568 | 84768816 | 84768568 | ID=MS.gene05531.t1.exon1;Parent=MS.gene05531.t1 |
| chr6.2 | CDS | 84768568 | 84768816 | 84768568 | ID=cds.MS.gene05531.t1;Parent=MS.gene05531.t1 |
| Gene Sequence |
| Protein sequence |
