Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84155.t1 | XP_003594956.1 | 95 | 179 | 9 | 0 | 1 | 179 | 1 | 179 | 3.20E-86 | 327.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84155.t1 | Q9SUM5 | 34.7 | 173 | 91 | 4 | 7 | 179 | 8 | 158 | 2.3e-16 | 87.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84155.t1 | G7IN04 | 95.0 | 179 | 9 | 0 | 1 | 179 | 1 | 179 | 2.3e-86 | 327.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050586 | MS.gene84155 | 0.805834 | 1.10E-49 | -1.69E-46 |
| MS.gene050787 | MS.gene84155 | 0.805885 | 1.08E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene84155 | 0.813374 | 2.64E-51 | -1.69E-46 |
| MS.gene057469 | MS.gene84155 | 0.810793 | 9.66E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene84155 | 0.831013 | 2.12E-55 | -1.69E-46 |
| MS.gene060404 | MS.gene84155 | 0.92448 | 6.66E-90 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84155.t1 | MTR_2g036670 | 94.972 | 179 | 9 | 0 | 1 | 179 | 1 | 179 | 1.73e-124 | 348 |
| MS.gene84155.t1 | MTR_4g127700 | 47.024 | 168 | 60 | 6 | 2 | 169 | 3 | 141 | 9.74e-41 | 135 |
| MS.gene84155.t1 | MTR_2g083470 | 49.275 | 69 | 33 | 1 | 20 | 86 | 6 | 74 | 3.21e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84155.t1 | AT2G18969 | 34.857 | 175 | 98 | 4 | 7 | 176 | 8 | 171 | 3.56e-27 | 101 |
| MS.gene84155.t1 | AT4G30180 | 34.659 | 176 | 87 | 4 | 7 | 179 | 8 | 158 | 1.92e-21 | 85.9 |
Find 24 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTATTAGCCTCAGATTT+TGG | 0.172199 | 2.3:+52026663 | None:intergenic |
| ATCTGAGGCTAATAAGATTT+TGG | 0.186879 | 2.3:-52026658 | MS.gene84155:CDS |
| GACAACACTTTCAACATTCT+TGG | 0.207032 | 2.3:+52026845 | None:intergenic |
| TTTGCTTGCACGACTTTGTT+TGG | 0.344592 | 2.3:+52026942 | None:intergenic |
| TCCTTGATCTTCATCAAAGC+TGG | 0.404897 | 2.3:+52026888 | None:intergenic |
| TTGGTTGAAGAAAATATTGA+TGG | 0.430271 | 2.3:-52026639 | MS.gene84155:CDS |
| GATGGTATGTGATGAGGAAA+TGG | 0.466333 | 2.3:-52026550 | MS.gene84155:CDS |
| TAATTATCAACAAAATGAAA+TGG | 0.471422 | 2.3:-52026715 | MS.gene84155:CDS |
| CTTCAAAAGGATGGTGAAGA+AGG | 0.478954 | 2.3:-52026750 | MS.gene84155:CDS |
| TGTAAGTTGTTTGCAAATGC+AGG | 0.519711 | 2.3:-52026508 | MS.gene84155:CDS |
| CATACCATCTCTTCTCCTCC+TGG | 0.531281 | 2.3:+52026564 | None:intergenic |
| AGAAGAGATGGTATGTGATG+AGG | 0.532629 | 2.3:-52026556 | MS.gene84155:CDS |
| ACCAGCTTTGATGAAGATCA+AGG | 0.546207 | 2.3:-52026889 | MS.gene84155:CDS |
| AAAATGAAGCTTCAAAAGGA+TGG | 0.553863 | 2.3:-52026759 | MS.gene84155:CDS |
| TGGTGATGAAGATGAGATTG+TGG | 0.568954 | 2.3:-52026619 | MS.gene84155:CDS |
| GTATGAAGTGGACATGGCAA+TGG | 0.582971 | 2.3:-52026820 | MS.gene84155:CDS |
| GATACCAGGAGGAGAAGAGA+TGG | 0.597097 | 2.3:-52026568 | MS.gene84155:CDS |
| TGTCTTAGAAAATTGATACC+AGG | 0.622054 | 2.3:-52026582 | MS.gene84155:CDS |
| ATGGTGAAGAAGGTTCATCA+AGG | 0.630817 | 2.3:-52026740 | MS.gene84155:CDS |
| AAGTGTTGTCAAGTATGAAG+TGG | 0.641147 | 2.3:-52026832 | MS.gene84155:CDS |
| TTCAACTTGTCTGATGAGTG+AGG | 0.643602 | 2.3:+52026469 | None:intergenic |
| AGATTGTGGATGACCAATTG+AGG | 0.657789 | 2.3:-52026605 | MS.gene84155:CDS |
| CTTAGAAAATTGATACCAGG+AGG | 0.680984 | 2.3:-52026579 | MS.gene84155:CDS |
| TGTCAAGTATGAAGTGGACA+TGG | 0.689538 | 2.3:-52026826 | MS.gene84155:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTGATTTCTATAAAAAAAA+TGG | - | chr2.3:52026770-52026789 | MS.gene84155:CDS | 15.0% |
| !! | TAATTATCAACAAAATGAAA+TGG | - | chr2.3:52026742-52026761 | MS.gene84155:CDS | 15.0% |
| !!! | TTTTTTTATAGAAATCAAGT+GGG | + | chr2.3:52026769-52026788 | None:intergenic | 15.0% |
| !!! | TTTTTTTTATAGAAATCAAG+TGG | + | chr2.3:52026770-52026789 | None:intergenic | 15.0% |
| ! | TCTAAAAATGAAGCTTCAAA+AGG | - | chr2.3:52026694-52026713 | MS.gene84155:CDS | 25.0% |
| ! | TTGGTTGAAGAAAATATTGA+TGG | - | chr2.3:52026818-52026837 | MS.gene84155:CDS | 25.0% |
| AAAAAATGGTCCAAAATCTG+AGG | - | chr2.3:52026784-52026803 | MS.gene84155:CDS | 30.0% | |
| AAAATGAAGCTTCAAAAGGA+TGG | - | chr2.3:52026698-52026717 | MS.gene84155:CDS | 30.0% | |
| TGTCTTAGAAAATTGATACC+AGG | - | chr2.3:52026875-52026894 | MS.gene84155:CDS | 30.0% | |
| !! | ATCTGAGGCTAATAAGATTT+TGG | - | chr2.3:52026799-52026818 | MS.gene84155:CDS | 30.0% |
| !! | ATCTTATTAGCCTCAGATTT+TGG | + | chr2.3:52026797-52026816 | None:intergenic | 30.0% |
| !!! | ATTTTCTAAGACACCTCAAT+TGG | + | chr2.3:52026868-52026887 | None:intergenic | 30.0% |
| CTTAGAAAATTGATACCAGG+AGG | - | chr2.3:52026878-52026897 | MS.gene84155:CDS | 35.0% | |
| GACAACACTTTCAACATTCT+TGG | + | chr2.3:52026615-52026634 | None:intergenic | 35.0% | |
| TGTAAGTTGTTTGCAAATGC+AGG | - | chr2.3:52026949-52026968 | MS.gene84155:CDS | 35.0% | |
| ! | AAGTGTTGTCAAGTATGAAG+TGG | - | chr2.3:52026625-52026644 | MS.gene84155:CDS | 35.0% |
| ! | TTTCATCTCAAGGTTTTGCA+TGG | - | chr2.3:52026663-52026682 | MS.gene84155:CDS | 35.0% |
| !!! | GCAATGGTTTTTTCATCTCA+AGG | - | chr2.3:52026653-52026672 | MS.gene84155:CDS | 35.0% |
| AGAAGAGATGGTATGTGATG+AGG | - | chr2.3:52026901-52026920 | MS.gene84155:CDS | 40.0% | |
| AGATTGTGGATGACCAATTG+AGG | - | chr2.3:52026852-52026871 | MS.gene84155:CDS | 40.0% | |
| ATGGTGAAGAAGGTTCATCA+AGG | - | chr2.3:52026717-52026736 | MS.gene84155:CDS | 40.0% | |
| CTTCAAAAGGATGGTGAAGA+AGG | - | chr2.3:52026707-52026726 | MS.gene84155:CDS | 40.0% | |
| GATGGTATGTGATGAGGAAA+TGG | - | chr2.3:52026907-52026926 | MS.gene84155:CDS | 40.0% | |
| TCCTTGATCTTCATCAAAGC+TGG | + | chr2.3:52026572-52026591 | None:intergenic | 40.0% | |
| TGTCAAGTATGAAGTGGACA+TGG | - | chr2.3:52026631-52026650 | MS.gene84155:CDS | 40.0% | |
| TTTGCTTGCACGACTTTGTT+TGG | + | chr2.3:52026518-52026537 | None:intergenic | 40.0% | |
| ! | ACCAGCTTTGATGAAGATCA+AGG | - | chr2.3:52026568-52026587 | MS.gene84155:CDS | 40.0% |
| ! | TGGTGATGAAGATGAGATTG+TGG | - | chr2.3:52026838-52026857 | MS.gene84155:CDS | 40.0% |
| GTATGAAGTGGACATGGCAA+TGG | - | chr2.3:52026637-52026656 | MS.gene84155:CDS | 45.0% | |
| CATACCATCTCTTCTCCTCC+TGG | + | chr2.3:52026896-52026915 | None:intergenic | 50.0% | |
| GATACCAGGAGGAGAAGAGA+TGG | - | chr2.3:52026889-52026908 | MS.gene84155:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 52026470 | 52027009 | 52026470 | ID=MS.gene84155 |
| chr2.3 | mRNA | 52026470 | 52027009 | 52026470 | ID=MS.gene84155.t1;Parent=MS.gene84155 |
| chr2.3 | exon | 52026470 | 52027009 | 52026470 | ID=MS.gene84155.t1.exon1;Parent=MS.gene84155.t1 |
| chr2.3 | CDS | 52026470 | 52027009 | 52026470 | ID=cds.MS.gene84155.t1;Parent=MS.gene84155.t1 |
| Gene Sequence |
| Protein sequence |
