Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060404.t1 | XP_003594956.1 | 95.5 | 179 | 8 | 0 | 1 | 179 | 1 | 179 | 1.30E-87 | 332.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060404.t1 | Q9SUM5 | 34.1 | 173 | 92 | 4 | 7 | 179 | 8 | 158 | 5.0e-16 | 85.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060404.t1 | G7IN04 | 95.5 | 179 | 8 | 0 | 1 | 179 | 1 | 179 | 9.3e-88 | 332.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene060404 | 0.810977 | 8.81E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene060404 | 0.808816 | 2.57E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene060404 | 0.814428 | 1.55E-51 | -1.69E-46 |
| MS.gene050586 | MS.gene060404 | 0.812312 | 4.52E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene060404 | 0.809856 | 1.54E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene060404 | 0.829665 | 4.53E-55 | -1.69E-46 |
| MS.gene060404 | MS.gene061618 | 0.808402 | 3.15E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene062429 | 0.820232 | 7.65E-53 | -1.69E-46 |
| MS.gene060404 | MS.gene06594 | 0.826477 | 2.65E-54 | -1.69E-46 |
| MS.gene060404 | MS.gene066941 | 0.806886 | 6.62E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene06705 | 0.807993 | 3.85E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene067234 | 0.821574 | 3.76E-53 | -1.69E-46 |
| MS.gene060404 | MS.gene06806 | 0.824497 | 7.82E-54 | -1.69E-46 |
| MS.gene060404 | MS.gene06922 | 0.807284 | 5.45E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene069868 | -0.806009 | 1.01E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene07089 | 0.817508 | 3.18E-52 | -1.69E-46 |
| MS.gene060404 | MS.gene07115 | 0.835217 | 1.91E-56 | -1.69E-46 |
| MS.gene060404 | MS.gene071290 | 0.801767 | 7.71E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene071438 | -0.805565 | 1.26E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene07263 | 0.824509 | 7.77E-54 | -1.69E-46 |
| MS.gene060404 | MS.gene09136 | 0.831276 | 1.83E-55 | -1.69E-46 |
| MS.gene060404 | MS.gene20784 | 0.82182 | 3.30E-53 | -1.69E-46 |
| MS.gene060404 | MS.gene20835 | 0.830513 | 2.82E-55 | -1.69E-46 |
| MS.gene060404 | MS.gene22854 | 0.814891 | 1.22E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene25889 | 0.806513 | 7.93E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene28405 | 0.800996 | 1.11E-48 | -1.69E-46 |
| MS.gene060404 | MS.gene28929 | 0.816225 | 6.17E-52 | -1.69E-46 |
| MS.gene060404 | MS.gene30964 | 0.833389 | 5.49E-56 | -1.69E-46 |
| MS.gene060404 | MS.gene31148 | 0.834596 | 2.74E-56 | -1.69E-46 |
| MS.gene060404 | MS.gene31519 | 0.811669 | 6.24E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene34106 | 0.820209 | 7.74E-53 | -1.69E-46 |
| MS.gene060404 | MS.gene36374 | 0.811747 | 6.00E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene38528 | 0.821959 | 3.06E-53 | -1.69E-46 |
| MS.gene060404 | MS.gene44007 | 0.832206 | 1.08E-55 | -1.69E-46 |
| MS.gene060404 | MS.gene44135 | -0.818287 | 2.12E-52 | -1.69E-46 |
| MS.gene060404 | MS.gene45498 | 0.837421 | 5.25E-57 | -1.69E-46 |
| MS.gene060404 | MS.gene47301 | 0.800025 | 1.75E-48 | -1.69E-46 |
| MS.gene060404 | MS.gene50595 | 0.819391 | 1.19E-52 | -1.69E-46 |
| MS.gene060404 | MS.gene55470 | 0.806504 | 7.97E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene56179 | 0.807265 | 5.50E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene56484 | 0.803472 | 3.43E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene56555 | 0.808389 | 3.17E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene57160 | -0.820573 | 6.39E-53 | -1.69E-46 |
| MS.gene060404 | MS.gene57162 | -0.834073 | 3.70E-56 | -1.69E-46 |
| MS.gene060404 | MS.gene57945 | 0.800409 | 1.46E-48 | -1.69E-46 |
| MS.gene060404 | MS.gene58092 | 0.84294 | 1.90E-58 | -1.69E-46 |
| MS.gene060404 | MS.gene58473 | 0.800442 | 1.44E-48 | -1.69E-46 |
| MS.gene060404 | MS.gene59876 | 0.816022 | 6.85E-52 | -1.69E-46 |
| MS.gene060404 | MS.gene59901 | 0.830719 | 2.51E-55 | -1.69E-46 |
| MS.gene060404 | MS.gene59903 | 0.800707 | 1.27E-48 | -1.69E-46 |
| MS.gene060404 | MS.gene62682 | 0.802971 | 4.35E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene62720 | 0.804542 | 2.05E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene63887 | 0.832782 | 7.77E-56 | -1.69E-46 |
| MS.gene060404 | MS.gene64621 | 0.812447 | 4.22E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene64923 | 0.807179 | 5.74E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene65665 | 0.803833 | 2.89E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene67002 | 0.811761 | 5.96E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene67384 | 0.804106 | 2.53E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene68764 | 0.811342 | 7.35E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene68873 | 0.800975 | 1.12E-48 | -1.69E-46 |
| MS.gene060404 | MS.gene68877 | 0.801785 | 7.64E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene68933 | 0.812104 | 5.01E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene68959 | 0.813494 | 2.49E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene69935 | 0.828907 | 6.92E-55 | -1.69E-46 |
| MS.gene060404 | MS.gene70237 | 0.839481 | 1.54E-57 | -1.69E-46 |
| MS.gene060404 | MS.gene70819 | 0.852263 | 5.18E-61 | -1.69E-46 |
| MS.gene060404 | MS.gene70989 | -0.809226 | 2.10E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene72557 | 0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene72558 | 0.801579 | 8.43E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene72851 | -0.811105 | 8.27E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene73227 | 0.805661 | 1.20E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene73273 | 0.814169 | 1.77E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene73333 | 0.996137 | 7.83E-224 | -1.69E-46 |
| MS.gene060404 | MS.gene74960 | 0.803125 | 4.05E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene75802 | 0.80274 | 4.86E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene76533 | -0.804411 | 2.19E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene77584 | -0.81397 | 1.95E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene78247 | 0.821338 | 4.26E-53 | -1.69E-46 |
| MS.gene060404 | MS.gene79290 | 0.820009 | 8.60E-53 | -1.69E-46 |
| MS.gene060404 | MS.gene81381 | 0.833631 | 4.77E-56 | -1.69E-46 |
| MS.gene060404 | MS.gene81398 | 0.814529 | 1.47E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene82040 | 0.801179 | 1.02E-48 | -1.69E-46 |
| MS.gene060404 | MS.gene84086 | 0.814085 | 1.84E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene84155 | 0.92448 | 6.66E-90 | -1.69E-46 |
| MS.gene060404 | MS.gene84396 | 0.84462 | 6.75E-59 | -1.69E-46 |
| MS.gene060404 | MS.gene84812 | 0.801809 | 7.56E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene85149 | -0.806629 | 7.50E-50 | -1.69E-46 |
| MS.gene060404 | MS.gene86678 | 0.804684 | 1.92E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene88087 | 0.814448 | 1.53E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene92548 | 0.803871 | 2.83E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene96810 | 0.811372 | 7.23E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060404.t1 | MTR_2g036670 | 95.531 | 179 | 8 | 0 | 1 | 179 | 1 | 179 | 4.88e-126 | 352 |
| MS.gene060404.t1 | MTR_4g127700 | 47.024 | 168 | 60 | 6 | 2 | 169 | 3 | 141 | 9.61e-42 | 138 |
| MS.gene060404.t1 | MTR_2g083470 | 52.174 | 69 | 31 | 1 | 20 | 86 | 6 | 74 | 3.24e-14 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060404.t1 | AT2G18969 | 34.857 | 175 | 98 | 4 | 7 | 176 | 8 | 171 | 1.06e-26 | 100 |
| MS.gene060404.t1 | AT4G30180 | 34.091 | 176 | 88 | 4 | 7 | 179 | 8 | 158 | 1.36e-20 | 83.6 |
Find 24 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTATTAGCCTCAGATTT+TGG | 0.172199 | 2.1:+53608663 | None:intergenic |
| ATCTGAGGCTAATAAGATTT+TGG | 0.186879 | 2.1:-53608658 | MS.gene060404:CDS |
| GACAATACTTTCAACATTCT+TGG | 0.219614 | 2.1:+53608845 | None:intergenic |
| TTTGCTTGCACTACTTTGTT+TGG | 0.314099 | 2.1:+53608942 | None:intergenic |
| TCCTTGATCTTCATCAAAGC+TGG | 0.404897 | 2.1:+53608888 | None:intergenic |
| TTGGTTGAAGAAAATATTGA+TGG | 0.430271 | 2.1:-53608639 | MS.gene060404:CDS |
| GATGGTATGTGATGAGGAAA+TGG | 0.466333 | 2.1:-53608550 | MS.gene060404:CDS |
| CTTCAAAAGGATGGTGATGA+AGG | 0.470664 | 2.1:-53608750 | MS.gene060404:CDS |
| TAATTATCAACAAAATGAAA+TGG | 0.471422 | 2.1:-53608715 | MS.gene060404:CDS |
| TGTAAGTTGTTTGCAAATGC+AGG | 0.519711 | 2.1:-53608508 | MS.gene060404:CDS |
| CATACCATCTCTTCTCCTCC+TGG | 0.531281 | 2.1:+53608564 | None:intergenic |
| AGAAGAGATGGTATGTGATG+AGG | 0.532629 | 2.1:-53608556 | MS.gene060404:CDS |
| ACCAGCTTTGATGAAGATCA+AGG | 0.546207 | 2.1:-53608889 | MS.gene060404:CDS |
| AAAATGAAGCTTCAAAAGGA+TGG | 0.553863 | 2.1:-53608759 | MS.gene060404:CDS |
| TGGTGATGAAGATGAGATTG+TGG | 0.568954 | 2.1:-53608619 | MS.gene060404:CDS |
| AAGTATTGTCAAGTATGAAG+TGG | 0.579515 | 2.1:-53608832 | MS.gene060404:CDS |
| GTATGAAGTGGACATGGCAA+TGG | 0.582971 | 2.1:-53608820 | MS.gene060404:CDS |
| GATACCAGGAGGAGAAGAGA+TGG | 0.597097 | 2.1:-53608568 | MS.gene060404:CDS |
| TGTCTTAGAAAATTGATACC+AGG | 0.622054 | 2.1:-53608582 | MS.gene060404:CDS |
| TGTCAAGTATGAAGTGGACA+TGG | 0.630889 | 2.1:-53608826 | MS.gene060404:CDS |
| ATGGTGATGAAGGTTCATCA+AGG | 0.637436 | 2.1:-53608740 | MS.gene060404:CDS |
| TTCAACTTGTCTGATGAGTG+AGG | 0.643602 | 2.1:+53608469 | None:intergenic |
| AGATTGTGGATGACCAATTG+AGG | 0.657789 | 2.1:-53608605 | MS.gene060404:CDS |
| CTTAGAAAATTGATACCAGG+AGG | 0.680984 | 2.1:-53608579 | MS.gene060404:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTGATTTCTATAAAAAAAA+TGG | - | chr2.1:53608770-53608789 | MS.gene060404:CDS | 15.0% |
| !! | TAATTATCAACAAAATGAAA+TGG | - | chr2.1:53608742-53608761 | MS.gene060404:CDS | 15.0% |
| !!! | TTTTTTTATAGAAATCAAGT+GGG | + | chr2.1:53608769-53608788 | None:intergenic | 15.0% |
| !!! | TTTTTTTTATAGAAATCAAG+TGG | + | chr2.1:53608770-53608789 | None:intergenic | 15.0% |
| ! | TCTAAAAATGAAGCTTCAAA+AGG | - | chr2.1:53608694-53608713 | MS.gene060404:CDS | 25.0% |
| ! | TTGGTTGAAGAAAATATTGA+TGG | - | chr2.1:53608818-53608837 | MS.gene060404:CDS | 25.0% |
| AAAAAATGGTCCAAAATCTG+AGG | - | chr2.1:53608784-53608803 | MS.gene060404:CDS | 30.0% | |
| AAAATGAAGCTTCAAAAGGA+TGG | - | chr2.1:53608698-53608717 | MS.gene060404:CDS | 30.0% | |
| GACAATACTTTCAACATTCT+TGG | + | chr2.1:53608615-53608634 | None:intergenic | 30.0% | |
| TGTCTTAGAAAATTGATACC+AGG | - | chr2.1:53608875-53608894 | MS.gene060404:CDS | 30.0% | |
| ! | AAGTATTGTCAAGTATGAAG+TGG | - | chr2.1:53608625-53608644 | MS.gene060404:CDS | 30.0% |
| !! | ATCTGAGGCTAATAAGATTT+TGG | - | chr2.1:53608799-53608818 | MS.gene060404:CDS | 30.0% |
| !! | ATCTTATTAGCCTCAGATTT+TGG | + | chr2.1:53608797-53608816 | None:intergenic | 30.0% |
| !!! | ATTTTCTAAGACACCTCAAT+TGG | + | chr2.1:53608868-53608887 | None:intergenic | 30.0% |
| CTTAGAAAATTGATACCAGG+AGG | - | chr2.1:53608878-53608897 | MS.gene060404:CDS | 35.0% | |
| TGTAAGTTGTTTGCAAATGC+AGG | - | chr2.1:53608949-53608968 | MS.gene060404:CDS | 35.0% | |
| TTTGCTTGCACTACTTTGTT+TGG | + | chr2.1:53608518-53608537 | None:intergenic | 35.0% | |
| ! | TTTCATCTCAAGGTTTTGCA+TGG | - | chr2.1:53608663-53608682 | MS.gene060404:CDS | 35.0% |
| !!! | GCAATGGTTTTTTCATCTCA+AGG | - | chr2.1:53608653-53608672 | MS.gene060404:CDS | 35.0% |
| AGAAGAGATGGTATGTGATG+AGG | - | chr2.1:53608901-53608920 | MS.gene060404:CDS | 40.0% | |
| AGATTGTGGATGACCAATTG+AGG | - | chr2.1:53608852-53608871 | MS.gene060404:CDS | 40.0% | |
| GATGGTATGTGATGAGGAAA+TGG | - | chr2.1:53608907-53608926 | MS.gene060404:CDS | 40.0% | |
| TCCTTGATCTTCATCAAAGC+TGG | + | chr2.1:53608572-53608591 | None:intergenic | 40.0% | |
| TGTCAAGTATGAAGTGGACA+TGG | - | chr2.1:53608631-53608650 | MS.gene060404:CDS | 40.0% | |
| ! | ACCAGCTTTGATGAAGATCA+AGG | - | chr2.1:53608568-53608587 | MS.gene060404:CDS | 40.0% |
| ! | ATGGTGATGAAGGTTCATCA+AGG | - | chr2.1:53608717-53608736 | MS.gene060404:CDS | 40.0% |
| ! | CTTCAAAAGGATGGTGATGA+AGG | - | chr2.1:53608707-53608726 | MS.gene060404:CDS | 40.0% |
| ! | TGGTGATGAAGATGAGATTG+TGG | - | chr2.1:53608838-53608857 | MS.gene060404:CDS | 40.0% |
| GTATGAAGTGGACATGGCAA+TGG | - | chr2.1:53608637-53608656 | MS.gene060404:CDS | 45.0% | |
| CATACCATCTCTTCTCCTCC+TGG | + | chr2.1:53608896-53608915 | None:intergenic | 50.0% | |
| GATACCAGGAGGAGAAGAGA+TGG | - | chr2.1:53608889-53608908 | MS.gene060404:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 53608470 | 53609009 | 53608470 | ID=MS.gene060404 |
| chr2.1 | mRNA | 53608470 | 53609009 | 53608470 | ID=MS.gene060404.t1;Parent=MS.gene060404 |
| chr2.1 | exon | 53608470 | 53609009 | 53608470 | ID=MS.gene060404.t1.exon1;Parent=MS.gene060404.t1 |
| chr2.1 | CDS | 53608470 | 53609009 | 53608470 | ID=cds.MS.gene060404.t1;Parent=MS.gene060404.t1 |
| Gene Sequence |
| Protein sequence |
