AlfalfaGEDB | MS.gene84324

M. sativa cultivar XinJiangDaYe / MS.gene84324

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84324.t1	XP_024633300.1	97.2	145	4	0	1	145	1	145	2.10E-64	255

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84324.t1	Q9M280	49.4	79	32	2	73	145	38	114	1.2e-10	67.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84324.t1	G7ITT2	94.5	145	8	0	1	145	1	145	9.2e-62	245.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05081	MS.gene84324	0.804051	2.60E-49	-1.69E-46
MS.gene051838	MS.gene84324	0.811519	6.72E-51	-1.69E-46
MS.gene052469	MS.gene84324	0.807099	5.96E-50	-1.69E-46
MS.gene052870	MS.gene84324	0.807244	5.56E-50	-1.69E-46
MS.gene052872	MS.gene84324	0.812825	3.49E-51	-1.69E-46
MS.gene054884	MS.gene84324	-0.81878	1.64E-52	-1.69E-46
MS.gene05494	MS.gene84324	0.809401	1.93E-50	-1.69E-46
MS.gene059717	MS.gene84324	0.813051	3.11E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84324.t1	MTR_2g086480	93.793	145	9	0	1	145	1	145	5.69e-91	266
MS.gene84324.t1	MTR_1g059990	55.000	60	26	1	71	130	49	107	2.06e-15	68.2
MS.gene84324.t1	MTR_7g104540	41.935	93	51	2	53	145	82	171	1.61e-14	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84324.t1	AT3G44450	49.367	79	32	2	73	145	38	114	3.76e-19	77.8
MS.gene84324.t1	AT3G52740	46.479	71	36	1	74	144	65	133	9.95e-17	72.0

Find 35 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTTTACTCTCTTCCTCTT+TGG	0.196605	2.2:+12121155	None:intergenic
ATTTACGTACATCAGCAATA+AGG	0.310452	2.2:+12120941	None:intergenic
AAATCAAGGTCACAAAGATT+AGG	0.365491	2.2:-12120922	MS.gene84324:CDS
ATAATAATGAAGTTGAAATA+TGG	0.373878	2.2:+12121262	None:intergenic
AGGTTAAGGTACCTGAAAAC+TGG	0.375495	2.2:-12121059	MS.gene84324:CDS
AATCAAGGTCACAAAGATTA+GGG	0.392972	2.2:-12120921	MS.gene84324:CDS
GAGGCATAGGGAAGAGATGA+AGG	0.397356	2.2:-12121085	MS.gene84324:CDS
TTTACGTACATCAGCAATAA+GGG	0.413998	2.2:+12120942	None:intergenic
TGCTAGGGTAGAGGAAATTA+CGG	0.414031	2.2:-12121124	MS.gene84324:CDS
AGAGATGAAGGGAAAGGTTA+AGG	0.420194	2.2:-12121073	MS.gene84324:CDS
GCTTGTAATCACTTTGTTGA+AGG	0.455949	2.2:+12121238	None:intergenic
AGGAACAAAAGCTGAAAGAA+TGG	0.465707	2.2:-12121032	MS.gene84324:CDS
AAGTTGAAATATGGCAAGTA+GGG	0.471669	2.2:+12121271	None:intergenic
GAAGTTGAAATATGGCAAGT+AGG	0.479728	2.2:+12121270	None:intergenic
GGAACAAAAGCTGAAAGAAT+GGG	0.483468	2.2:-12121031	MS.gene84324:CDS
CTTGTAATCACTTTGTTGAA+GGG	0.500090	2.2:+12121239	None:intergenic
GCTAGGGTAGAGGAAATTAC+GGG	0.501686	2.2:-12121123	MS.gene84324:CDS
GTAAAGAGAATGGCACTGCT+AGG	0.508070	2.2:-12121140	MS.gene84324:CDS
GAGGAAGAGAGTAAAGAGAA+TGG	0.521873	2.2:-12121150	MS.gene84324:CDS
GAATGGCACTGCTAGGGTAG+AGG	0.532050	2.2:-12121133	MS.gene84324:CDS
GTGAGAGGTTGAAGAGGCAT+AGG	0.534191	2.2:-12121098	MS.gene84324:CDS
TAAAGAGAATGGCACTGCTA+GGG	0.541222	2.2:-12121139	MS.gene84324:CDS
TTGCTGATGTACGTAAATCA+AGG	0.545508	2.2:-12120936	MS.gene84324:CDS
AGGAAATTACGGGGCGTGAG+AGG	0.563528	2.2:-12121113	MS.gene84324:CDS
TAGGGAAGAGATGAAGGGAA+AGG	0.571304	2.2:-12121079	MS.gene84324:CDS
GGTTAAGGTACCTGAAAACT+GGG	0.585046	2.2:-12121058	MS.gene84324:CDS
AGGCATAGGGAAGAGATGAA+GGG	0.595639	2.2:-12121084	MS.gene84324:CDS
TGAGAGGTTGAAGAGGCATA+GGG	0.598492	2.2:-12121097	MS.gene84324:CDS
TCAAAATAAGAAACTAGCTG+TGG	0.599837	2.2:+12120896	None:intergenic
TGATGATGAAAAGGCCAAAG+AGG	0.600501	2.2:-12121169	MS.gene84324:CDS
GGTACCTGAAAACTGGGAGA+AGG	0.618394	2.2:-12121052	MS.gene84324:CDS
CGGGGCGTGAGAGGTTGAAG+AGG	0.628226	2.2:-12121104	MS.gene84324:CDS
GCAGTAACAATCAATGCATG+AGG	0.659977	2.2:+12120973	None:intergenic
TGACTCATAACATCTCAACA+TGG	0.722472	2.2:-12121305	MS.gene84324:CDS
CTAGGGTAGAGGAAATTACG+GGG	0.760541	2.2:-12121122	MS.gene84324:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATAATGAAGTTGAAATA+TGG	+	chr2.2:12120974-12120993	None:intergenic	15.0%
!!	TCATCATAGCTTTTCTTATT+TGG	+	chr2.2:12121035-12121054	None:intergenic	25.0%
	AAATCAAGGTCACAAAGATT+AGG	-	chr2.2:12121311-12121330	MS.gene84324:CDS	30.0%
	AAGTTGAAATATGGCAAGTA+GGG	+	chr2.2:12120965-12120984	None:intergenic	30.0%
	AATCAAGGTCACAAAGATTA+GGG	-	chr2.2:12121312-12121331	MS.gene84324:CDS	30.0%
	ATTTACGTACATCAGCAATA+AGG	+	chr2.2:12121295-12121314	None:intergenic	30.0%
	CTTGTAATCACTTTGTTGAA+GGG	+	chr2.2:12120997-12121016	None:intergenic	30.0%
	TTTACGTACATCAGCAATAA+GGG	+	chr2.2:12121294-12121313	None:intergenic	30.0%
!!	GATTGATGATGATGATGAAA+AGG	-	chr2.2:12121055-12121074	MS.gene84324:CDS	30.0%
	AGGAACAAAAGCTGAAAGAA+TGG	-	chr2.2:12121201-12121220	MS.gene84324:CDS	35.0%
	GAAGTTGAAATATGGCAAGT+AGG	+	chr2.2:12120966-12120985	None:intergenic	35.0%
	GCTTGTAATCACTTTGTTGA+AGG	+	chr2.2:12120998-12121017	None:intergenic	35.0%
	GGAACAAAAGCTGAAAGAAT+GGG	-	chr2.2:12121202-12121221	MS.gene84324:CDS	35.0%
	TGACTCATAACATCTCAACA+TGG	-	chr2.2:12120928-12120947	MS.gene84324:CDS	35.0%
	TTGCTGATGTACGTAAATCA+AGG	-	chr2.2:12121297-12121316	MS.gene84324:CDS	35.0%
	AGAGATGAAGGGAAAGGTTA+AGG	-	chr2.2:12121160-12121179	MS.gene84324:CDS	40.0%
	CTCTTTACTCTCTTCCTCTT+TGG	+	chr2.2:12121081-12121100	None:intergenic	40.0%
	GAGGAAGAGAGTAAAGAGAA+TGG	-	chr2.2:12121083-12121102	MS.gene84324:CDS	40.0%
	GCAGTAACAATCAATGCATG+AGG	+	chr2.2:12121263-12121282	None:intergenic	40.0%
	TAAAGAGAATGGCACTGCTA+GGG	-	chr2.2:12121094-12121113	MS.gene84324:CDS	40.0%
	TGCTAGGGTAGAGGAAATTA+CGG	-	chr2.2:12121109-12121128	MS.gene84324:CDS	40.0%
!	AGGTTAAGGTACCTGAAAAC+TGG	-	chr2.2:12121174-12121193	MS.gene84324:CDS	40.0%
!	GGTTAAGGTACCTGAAAACT+GGG	-	chr2.2:12121175-12121194	MS.gene84324:CDS	40.0%
!	TGATGATGAAAAGGCCAAAG+AGG	-	chr2.2:12121064-12121083	MS.gene84324:CDS	40.0%
	AGGCATAGGGAAGAGATGAA+GGG	-	chr2.2:12121149-12121168	MS.gene84324:CDS	45.0%
	CTAGGGTAGAGGAAATTACG+GGG	-	chr2.2:12121111-12121130	MS.gene84324:CDS	45.0%
	GCTAGGGTAGAGGAAATTAC+GGG	-	chr2.2:12121110-12121129	MS.gene84324:CDS	45.0%
	GTAAAGAGAATGGCACTGCT+AGG	-	chr2.2:12121093-12121112	MS.gene84324:CDS	45.0%
	TAGGGAAGAGATGAAGGGAA+AGG	-	chr2.2:12121154-12121173	MS.gene84324:CDS	45.0%
!	TGAGAGGTTGAAGAGGCATA+GGG	-	chr2.2:12121136-12121155	MS.gene84324:CDS	45.0%
!	TGTTCCTTCTCCCAGTTTTC+AGG	+	chr2.2:12121188-12121207	None:intergenic	45.0%
	GAGGCATAGGGAAGAGATGA+AGG	-	chr2.2:12121148-12121167	MS.gene84324:CDS	50.0%
	GGTACCTGAAAACTGGGAGA+AGG	-	chr2.2:12121181-12121200	MS.gene84324:CDS	50.0%
!	GTGAGAGGTTGAAGAGGCAT+AGG	-	chr2.2:12121135-12121154	MS.gene84324:CDS	50.0%
	AGGAAATTACGGGGCGTGAG+AGG	-	chr2.2:12121120-12121139	MS.gene84324:CDS	55.0%
	GAATGGCACTGCTAGGGTAG+AGG	-	chr2.2:12121100-12121119	MS.gene84324:CDS	55.0%
	CGGGGCGTGAGAGGTTGAAG+AGG	-	chr2.2:12121129-12121148	MS.gene84324:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	12120909	12121346	12120909	ID=MS.gene84324
chr2.2	mRNA	12120909	12121346	12120909	ID=MS.gene84324.t1;Parent=MS.gene84324
chr2.2	exon	12120909	12121346	12120909	ID=MS.gene84324.t1.exon1;Parent=MS.gene84324.t1
chr2.2	CDS	12120909	12121346	12120909	ID=cds.MS.gene84324.t1;Parent=MS.gene84324.t1

Gene Sequence

>MS.gene84324

ATGGATCAGGAAAATAGATTGACTCATAACATCTCAACATGGAAGAGAAGAAACCCTACTTGCCATATTTCAACTTCATTATTATCCCTTCAACAAAGTGATTACAAGCTCGAGTGCAACTCTCCAAATAAGAAAAGCTATGATGAGATTGATGATGATGATGAAAAGGCCAAAGAGGAAGAGAGTAAAGAGAATGGCACTGCTAGGGTAGAGGAAATTACGGGGCGTGAGAGGTTGAAGAGGCATAGGGAAGAGATGAAGGGAAAGGTTAAGGTACCTGAAAACTGGGAGAAGGAACAAAAGCTGAAAGAATGGGTTGACTATACTACGTTTGATGCATTGATATTTGCTCCTCATGCATTGATTGTTACTGCTCGTGATGCCCTTATTGCTGATGTACGTAAATCAAGGTCACAAAGATTAGGGATCCACAGCTAG

Protein sequence

>MS.gene84324.t1

MDQENRLTHNISTWKRRNPTCHISTSLLSLQQSDYKLECNSPNKKSYDEIDDDDEKAKEEESKENGTARVEEITGRERLKRHREEMKGKVKVPENWEKEQKLKEWVDYTTFDALIFAPHALIVTARDALIADVRKSRSQRLGIHS