Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84367.t1 | XP_003598238.1 | 95.7 | 186 | 8 | 0 | 1 | 186 | 1 | 186 | 4.70E-93 | 350.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84367.t1 | P17407 | 52.2 | 186 | 87 | 2 | 3 | 186 | 6 | 191 | 2.3e-48 | 193.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84367.t1 | G7IYJ2 | 95.7 | 186 | 8 | 0 | 1 | 186 | 1 | 186 | 3.4e-93 | 350.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene84367 | MS.gene77725 | PPI |
| MS.gene045669 | MS.gene84367 | PPI |
| MS.gene045671 | MS.gene84367 | PPI |
| MS.gene78072 | MS.gene84367 | PPI |
| MS.gene84367 | MS.gene72495 | PPI |
| MS.gene72493 | MS.gene84367 | PPI |
| MS.gene77727 | MS.gene84367 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84367.t1 | MTR_3g008820 | 95.699 | 186 | 8 | 0 | 1 | 186 | 1 | 186 | 1.15e-126 | 354 |
| MS.gene84367.t1 | MTR_3g008840 | 90.116 | 172 | 17 | 0 | 15 | 186 | 16 | 187 | 1.68e-117 | 331 |
| MS.gene84367.t1 | MTR_5g012000 | 48.649 | 185 | 93 | 2 | 4 | 186 | 27 | 211 | 3.15e-64 | 197 |
| MS.gene84367.t1 | MTR_4g101760 | 46.597 | 191 | 93 | 2 | 5 | 186 | 12 | 202 | 4.99e-58 | 181 |
| MS.gene84367.t1 | MTR_4g035905 | 49.080 | 163 | 81 | 1 | 24 | 186 | 36 | 196 | 2.25e-50 | 161 |
| MS.gene84367.t1 | MTR_4g035870 | 46.626 | 163 | 85 | 1 | 24 | 186 | 36 | 196 | 3.63e-45 | 148 |
| MS.gene84367.t1 | MTR_4g035880 | 46.012 | 163 | 86 | 1 | 24 | 186 | 36 | 196 | 5.02e-44 | 145 |
| MS.gene84367.t1 | MTR_4g025550 | 42.051 | 195 | 101 | 6 | 3 | 186 | 8 | 201 | 2.53e-43 | 144 |
| MS.gene84367.t1 | MTR_4g025590 | 42.690 | 171 | 89 | 5 | 24 | 186 | 3 | 172 | 1.51e-39 | 133 |
| MS.gene84367.t1 | MTR_4g025610 | 40.000 | 195 | 105 | 6 | 3 | 186 | 8 | 201 | 3.62e-39 | 133 |
| MS.gene84367.t1 | MTR_4g025620 | 39.109 | 202 | 106 | 6 | 1 | 186 | 1 | 201 | 5.03e-39 | 132 |
| MS.gene84367.t1 | MTR_4g120825 | 36.735 | 196 | 102 | 5 | 8 | 183 | 9 | 202 | 9.71e-36 | 124 |
| MS.gene84367.t1 | MTR_4g120830 | 36.735 | 196 | 102 | 5 | 8 | 183 | 9 | 202 | 1.53e-35 | 124 |
| MS.gene84367.t1 | MTR_8g104765 | 37.500 | 176 | 98 | 4 | 18 | 183 | 38 | 211 | 3.66e-34 | 120 |
| MS.gene84367.t1 | MTR_3g008760 | 35.165 | 182 | 104 | 4 | 12 | 181 | 45 | 224 | 3.83e-31 | 113 |
| MS.gene84367.t1 | MTR_2g043560 | 38.554 | 166 | 86 | 4 | 23 | 183 | 33 | 187 | 1.31e-29 | 108 |
| MS.gene84367.t1 | MTR_7g108790 | 32.727 | 165 | 107 | 3 | 17 | 181 | 26 | 186 | 2.68e-29 | 107 |
| MS.gene84367.t1 | MTR_4g124855 | 32.979 | 188 | 112 | 5 | 5 | 183 | 15 | 197 | 2.65e-25 | 97.4 |
| MS.gene84367.t1 | MTR_1g088540 | 30.822 | 146 | 97 | 2 | 39 | 184 | 1 | 142 | 8.82e-23 | 89.4 |
| MS.gene84367.t1 | MTR_4g035855 | 32.335 | 167 | 99 | 5 | 24 | 183 | 43 | 202 | 1.51e-19 | 82.4 |
| MS.gene84367.t1 | MTR_1g008140 | 30.000 | 180 | 107 | 5 | 19 | 185 | 76 | 249 | 1.88e-15 | 73.9 |
| MS.gene84367.t1 | MTR_8g023310 | 28.205 | 156 | 109 | 2 | 25 | 178 | 68 | 222 | 1.59e-14 | 71.2 |
| MS.gene84367.t1 | MTR_8g023150 | 30.723 | 166 | 104 | 5 | 20 | 181 | 72 | 230 | 4.90e-14 | 69.7 |
| MS.gene84367.t1 | MTR_3g091380 | 29.341 | 167 | 112 | 3 | 21 | 183 | 74 | 238 | 2.89e-13 | 67.4 |
| MS.gene84367.t1 | MTR_4g005170 | 26.250 | 160 | 112 | 4 | 25 | 183 | 63 | 217 | 1.67e-12 | 65.5 |
| MS.gene84367.t1 | MTR_1g083490 | 26.994 | 163 | 105 | 5 | 25 | 182 | 63 | 216 | 6.34e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84367.t1 | AT5G62350 | 54.651 | 172 | 76 | 2 | 17 | 186 | 31 | 202 | 9.97e-67 | 203 |
| MS.gene84367.t1 | AT3G47380 | 48.969 | 194 | 87 | 4 | 5 | 186 | 9 | 202 | 5.93e-63 | 193 |
| MS.gene84367.t1 | AT4G25260 | 45.771 | 201 | 94 | 2 | 1 | 186 | 1 | 201 | 4.76e-59 | 183 |
| MS.gene84367.t1 | AT1G62770 | 48.404 | 188 | 88 | 4 | 5 | 183 | 9 | 196 | 1.39e-54 | 172 |
| MS.gene84367.t1 | AT4G12390 | 49.102 | 167 | 82 | 1 | 23 | 186 | 38 | 204 | 6.12e-50 | 160 |
| MS.gene84367.t1 | AT5G62360 | 43.293 | 164 | 90 | 2 | 23 | 186 | 42 | 202 | 1.48e-48 | 157 |
| MS.gene84367.t1 | AT5G20740 | 45.062 | 162 | 85 | 2 | 23 | 181 | 32 | 192 | 1.13e-41 | 139 |
| MS.gene84367.t1 | AT5G20740 | 45.062 | 162 | 85 | 2 | 23 | 181 | 66 | 226 | 3.00e-41 | 139 |
| MS.gene84367.t1 | AT1G14890 | 36.508 | 189 | 106 | 3 | 6 | 185 | 34 | 217 | 1.18e-39 | 134 |
| MS.gene84367.t1 | AT4G00080 | 38.693 | 199 | 100 | 8 | 2 | 181 | 3 | 198 | 2.40e-39 | 133 |
| MS.gene84367.t1 | AT2G01610 | 36.979 | 192 | 103 | 4 | 10 | 185 | 30 | 219 | 4.17e-37 | 128 |
| MS.gene84367.t1 | AT1G62760 | 43.452 | 168 | 88 | 3 | 20 | 184 | 143 | 306 | 4.04e-36 | 128 |
| MS.gene84367.t1 | AT4G25250 | 35.185 | 162 | 104 | 1 | 22 | 182 | 35 | 196 | 8.94e-36 | 124 |
| MS.gene84367.t1 | AT1G70720 | 35.567 | 194 | 110 | 5 | 1 | 183 | 17 | 206 | 3.23e-31 | 112 |
| MS.gene84367.t1 | AT1G70720 | 35.567 | 194 | 110 | 5 | 1 | 183 | 5 | 194 | 3.39e-31 | 112 |
| MS.gene84367.t1 | AT1G23205 | 34.031 | 191 | 111 | 4 | 5 | 181 | 9 | 198 | 3.52e-30 | 110 |
| MS.gene84367.t1 | AT5G51520 | 35.366 | 164 | 97 | 2 | 24 | 179 | 37 | 199 | 1.24e-28 | 105 |
| MS.gene84367.t1 | AT3G62820 | 27.835 | 194 | 125 | 6 | 1 | 185 | 5 | 192 | 2.09e-20 | 84.3 |
| MS.gene84367.t1 | AT3G14310 | 32.911 | 158 | 100 | 4 | 25 | 179 | 59 | 213 | 4.31e-20 | 87.4 |
| MS.gene84367.t1 | AT5G62340 | 29.902 | 204 | 109 | 8 | 6 | 186 | 14 | 206 | 4.74e-19 | 81.3 |
| MS.gene84367.t1 | AT5G53370 | 30.061 | 163 | 107 | 4 | 18 | 179 | 104 | 260 | 6.11e-18 | 81.3 |
| MS.gene84367.t1 | AT5G53370 | 30.061 | 163 | 107 | 4 | 18 | 179 | 68 | 224 | 7.79e-18 | 80.9 |
| MS.gene84367.t1 | AT1G53830 | 28.834 | 163 | 114 | 2 | 18 | 179 | 55 | 216 | 1.08e-17 | 80.5 |
| MS.gene84367.t1 | AT3G49220 | 31.677 | 161 | 100 | 4 | 21 | 180 | 128 | 279 | 1.64e-17 | 79.7 |
| MS.gene84367.t1 | AT2G47670 | 28.125 | 160 | 112 | 2 | 24 | 183 | 48 | 204 | 2.09e-17 | 76.6 |
| MS.gene84367.t1 | AT3G49220 | 31.875 | 160 | 99 | 4 | 21 | 179 | 128 | 278 | 2.27e-17 | 79.3 |
| MS.gene84367.t1 | AT3G49220 | 31.875 | 160 | 99 | 4 | 21 | 179 | 83 | 233 | 2.32e-17 | 79.3 |
| MS.gene84367.t1 | AT3G47670 | 29.851 | 201 | 112 | 8 | 1 | 181 | 76 | 267 | 6.90e-14 | 68.6 |
| MS.gene84367.t1 | AT3G14300 | 28.177 | 181 | 116 | 6 | 12 | 182 | 258 | 434 | 9.46e-13 | 66.2 |
| MS.gene84367.t1 | AT3G14300 | 29.070 | 172 | 104 | 6 | 20 | 181 | 454 | 617 | 2.19e-12 | 65.1 |
Find 31 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCACTCATTAATCAATA+TGG | 0.347778 | 3.1:-4301185 | MS.gene84367:CDS |
| CCAATTGATGAGGGTCTTGT+TGG | 0.397480 | 3.1:+4301564 | None:intergenic |
| GGCTAATTGATAAAGCTGTT+TGG | 0.400503 | 3.1:+4301540 | None:intergenic |
| TAGGCTTAGCCGTTCACTTA+AGG | 0.410233 | 3.1:-4301399 | MS.gene84367:CDS |
| GAGGATTTGATGAAATTTGT+TGG | 0.411028 | 3.1:+4301650 | None:intergenic |
| AACAAAACTCAATCCACAAA+AGG | 0.414225 | 3.1:-4301515 | MS.gene84367:CDS |
| TCAATGGTCAAGATTTCAAT+TGG | 0.421550 | 3.1:-4301358 | MS.gene84367:CDS |
| AACCATTCTTGATCTAATAG+AGG | 0.423736 | 3.1:+4301235 | None:intergenic |
| AAATTACTGATAGTGTTGAT+AGG | 0.448838 | 3.1:-4301418 | MS.gene84367:CDS |
| CTTAAACTTTGCAAGATCAA+TGG | 0.453176 | 3.1:-4301374 | MS.gene84367:CDS |
| CCAACAAGACCCTCATCAAT+TGG | 0.458410 | 3.1:-4301564 | MS.gene84367:CDS |
| GAGTTGAAGTTGCAATAGAT+AGG | 0.465044 | 3.1:+4301672 | None:intergenic |
| TGAATAGCAGCATATTCTCT+TGG | 0.472801 | 3.1:+4301455 | None:intergenic |
| GAGATTCAACACAAAGTGTT+GGG | 0.484787 | 3.1:+4301609 | None:intergenic |
| AAGAAGAATATTTGTTGCCA+TGG | 0.488179 | 3.1:+4301712 | None:intergenic |
| AGTACTTGTAGTGATGGATT+TGG | 0.488408 | 3.1:-4301287 | MS.gene84367:CDS |
| AACCTCTATTAGATCAAGAA+TGG | 0.496455 | 3.1:-4301237 | MS.gene84367:CDS |
| AAGAATGGTTAATCTTGCTC+AGG | 0.503787 | 3.1:-4301222 | MS.gene84367:CDS |
| AGAGATTCAACACAAAGTGT+TGG | 0.533099 | 3.1:+4301608 | None:intergenic |
| ACAAAACTCAATCCACAAAA+GGG | 0.537573 | 3.1:-4301514 | MS.gene84367:CDS |
| TAGATAGGAGAGTTAAGAGA+AGG | 0.542534 | 3.1:+4301687 | None:intergenic |
| GTTTAAGCTCCTTAAGTGAA+CGG | 0.545857 | 3.1:+4301390 | None:intergenic |
| ACTTGTAGTGATGGATTTGG+TGG | 0.550252 | 3.1:-4301284 | MS.gene84367:CDS |
| TCAATATGGTACTAATCACT+AGG | 0.550549 | 3.1:-4301171 | None:intergenic |
| CAATATGGTACTAATCACTA+GGG | 0.573803 | 3.1:-4301170 | None:intergenic |
| ATATAAGTAATGTTCAAACA+TGG | 0.577147 | 3.1:-4301334 | MS.gene84367:CDS |
| CTGTTTGGACCAATTGATGA+GGG | 0.590554 | 3.1:+4301555 | None:intergenic |
| GATGCAAGTACTTGTAGTGA+TGG | 0.592136 | 3.1:-4301293 | MS.gene84367:CDS |
| TATAAGTAATGTTCAAACAT+GGG | 0.606584 | 3.1:-4301333 | MS.gene84367:CDS |
| GCTGTTTGGACCAATTGATG+AGG | 0.633670 | 3.1:+4301554 | None:intergenic |
| GTATGATGTTGTGCTACACG+AGG | 0.732899 | 3.1:+4301631 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAAGTAATGTTCAAACA+TGG | - | chr3.1:4301548-4301567 | MS.gene84367:CDS | 20.0% |
| !! | TATAAGTAATGTTCAAACAT+GGG | - | chr3.1:4301549-4301568 | MS.gene84367:CDS | 20.0% |
| ! | ACAATGTGCAAAAAGTTTAA+AGG | - | chr3.1:4301397-4301416 | MS.gene84367:CDS | 25.0% |
| !!! | AAATTACTGATAGTGTTGAT+AGG | - | chr3.1:4301464-4301483 | MS.gene84367:CDS | 25.0% |
| AACAAAACTCAATCCACAAA+AGG | - | chr3.1:4301367-4301386 | MS.gene84367:CDS | 30.0% | |
| AACCATTCTTGATCTAATAG+AGG | + | chr3.1:4301650-4301669 | None:intergenic | 30.0% | |
| AACCTCTATTAGATCAAGAA+TGG | - | chr3.1:4301645-4301664 | MS.gene84367:CDS | 30.0% | |
| ACAAAACTCAATCCACAAAA+GGG | - | chr3.1:4301368-4301387 | MS.gene84367:CDS | 30.0% | |
| CTTAAACTTTGCAAGATCAA+TGG | - | chr3.1:4301508-4301527 | MS.gene84367:CDS | 30.0% | |
| CTTGCACTCATTAATCAATA+TGG | - | chr3.1:4301697-4301716 | MS.gene84367:CDS | 30.0% | |
| GAGGATTTGATGAAATTTGT+TGG | + | chr3.1:4301235-4301254 | None:intergenic | 30.0% | |
| TCAATGGTCAAGATTTCAAT+TGG | - | chr3.1:4301524-4301543 | MS.gene84367:CDS | 30.0% | |
| !!! | TTGTGGATTGAGTTTTGTTT+AGG | + | chr3.1:4301366-4301385 | None:intergenic | 30.0% |
| AAGAATGGTTAATCTTGCTC+AGG | - | chr3.1:4301660-4301679 | MS.gene84367:CDS | 35.0% | |
| AGAGATTCAACACAAAGTGT+TGG | + | chr3.1:4301277-4301296 | None:intergenic | 35.0% | |
| GAGTTGAAGTTGCAATAGAT+AGG | + | chr3.1:4301213-4301232 | None:intergenic | 35.0% | |
| GGCTAATTGATAAAGCTGTT+TGG | + | chr3.1:4301345-4301364 | None:intergenic | 35.0% | |
| GTTTAAGCTCCTTAAGTGAA+CGG | + | chr3.1:4301495-4301514 | None:intergenic | 35.0% | |
| TAGATAGGAGAGTTAAGAGA+AGG | + | chr3.1:4301198-4301217 | None:intergenic | 35.0% | |
| TGAATAGCAGCATATTCTCT+TGG | + | chr3.1:4301430-4301449 | None:intergenic | 35.0% | |
| TTGGTGGAAAAACACTTGAT+GGG | - | chr3.1:4301614-4301633 | MS.gene84367:CDS | 35.0% | |
| TTTGGTGGAAAAACACTTGA+TGG | - | chr3.1:4301613-4301632 | MS.gene84367:CDS | 35.0% | |
| ! | CATTGTAACAAACCCTTTTG+TGG | + | chr3.1:4301383-4301402 | None:intergenic | 35.0% |
| ! | GAGATTCAACACAAAGTGTT+GGG | + | chr3.1:4301276-4301295 | None:intergenic | 35.0% |
| !! | AGTACTTGTAGTGATGGATT+TGG | - | chr3.1:4301595-4301614 | MS.gene84367:CDS | 35.0% |
| CTGTTTGGACCAATTGATGA+GGG | + | chr3.1:4301330-4301349 | None:intergenic | 40.0% | |
| GATGCAAGTACTTGTAGTGA+TGG | - | chr3.1:4301589-4301608 | MS.gene84367:CDS | 40.0% | |
| !! | ACTTGTAGTGATGGATTTGG+TGG | - | chr3.1:4301598-4301617 | MS.gene84367:CDS | 40.0% |
| CCAACAAGACCCTCATCAAT+TGG | - | chr3.1:4301318-4301337 | MS.gene84367:CDS | 45.0% | |
| GCTGTTTGGACCAATTGATG+AGG | + | chr3.1:4301331-4301350 | None:intergenic | 45.0% | |
| GTATGATGTTGTGCTACACG+AGG | + | chr3.1:4301254-4301273 | None:intergenic | 45.0% | |
| TAGGCTTAGCCGTTCACTTA+AGG | - | chr3.1:4301483-4301502 | MS.gene84367:CDS | 45.0% | |
| ! | CCAATTGATGAGGGTCTTGT+TGG | + | chr3.1:4301321-4301340 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 4301172 | 4301732 | 4301172 | ID=MS.gene84367 |
| chr3.1 | mRNA | 4301172 | 4301732 | 4301172 | ID=MS.gene84367.t1;Parent=MS.gene84367 |
| chr3.1 | exon | 4301172 | 4301732 | 4301172 | ID=MS.gene84367.t1.exon1;Parent=MS.gene84367.t1 |
| chr3.1 | CDS | 4301172 | 4301732 | 4301172 | ID=cds.MS.gene84367.t1;Parent=MS.gene84367.t1 |
| Gene Sequence |
| Protein sequence |
