Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84369.t1 | XP_003598238.1 | 94.5 | 109 | 6 | 0 | 2 | 110 | 78 | 186 | 3.10E-52 | 214.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84369.t1 | P17407 | 53.3 | 107 | 49 | 1 | 5 | 110 | 85 | 191 | 9.3e-29 | 127.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84369.t1 | B7FGI2 | 94.5 | 109 | 6 | 0 | 2 | 110 | 78 | 186 | 2.3e-52 | 214.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene84369 | MS.gene77727 | PPI |
| MS.gene84369 | MS.gene72495 | PPI |
| MS.gene84369 | MS.gene045671 | PPI |
| MS.gene84369 | MS.gene77725 | PPI |
| MS.gene84369 | MS.gene045669 | PPI |
| MS.gene84369 | MS.gene78072 | PPI |
| MS.gene84369 | MS.gene72493 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84369.t1 | MTR_3g008820 | 94.495 | 109 | 6 | 0 | 2 | 110 | 78 | 186 | 1.15e-75 | 221 |
| MS.gene84369.t1 | MTR_3g008840 | 86.239 | 109 | 15 | 0 | 2 | 110 | 79 | 187 | 1.49e-69 | 206 |
| MS.gene84369.t1 | MTR_5g012000 | 50.943 | 106 | 51 | 1 | 6 | 110 | 106 | 211 | 7.99e-39 | 129 |
| MS.gene84369.t1 | MTR_4g101760 | 50.000 | 106 | 52 | 1 | 6 | 110 | 97 | 202 | 1.71e-35 | 120 |
| MS.gene84369.t1 | MTR_4g035905 | 51.429 | 105 | 49 | 1 | 6 | 110 | 94 | 196 | 2.51e-35 | 120 |
| MS.gene84369.t1 | MTR_4g035870 | 48.571 | 105 | 52 | 1 | 6 | 110 | 94 | 196 | 1.27e-30 | 107 |
| MS.gene84369.t1 | MTR_4g035880 | 47.619 | 105 | 53 | 1 | 6 | 110 | 94 | 196 | 1.53e-29 | 105 |
| MS.gene84369.t1 | MTR_4g025610 | 47.115 | 104 | 50 | 3 | 11 | 110 | 99 | 201 | 1.97e-25 | 95.1 |
| MS.gene84369.t1 | MTR_4g025590 | 47.115 | 104 | 50 | 3 | 11 | 110 | 70 | 172 | 4.14e-25 | 93.6 |
| MS.gene84369.t1 | MTR_4g025550 | 46.154 | 104 | 51 | 3 | 11 | 110 | 99 | 201 | 1.60e-24 | 92.8 |
| MS.gene84369.t1 | MTR_4g025620 | 46.154 | 104 | 51 | 3 | 11 | 110 | 99 | 201 | 1.72e-24 | 92.8 |
| MS.gene84369.t1 | MTR_2g043560 | 44.000 | 100 | 46 | 2 | 8 | 107 | 98 | 187 | 3.54e-21 | 84.0 |
| MS.gene84369.t1 | MTR_4g124855 | 38.144 | 97 | 58 | 1 | 11 | 107 | 103 | 197 | 6.62e-18 | 75.5 |
| MS.gene84369.t1 | MTR_1g088540 | 38.614 | 101 | 58 | 2 | 8 | 108 | 46 | 142 | 3.98e-17 | 72.0 |
| MS.gene84369.t1 | MTR_4g120825 | 36.036 | 111 | 62 | 3 | 4 | 107 | 94 | 202 | 2.02e-16 | 71.6 |
| MS.gene84369.t1 | MTR_8g104765 | 36.036 | 111 | 62 | 3 | 4 | 107 | 103 | 211 | 2.57e-16 | 71.6 |
| MS.gene84369.t1 | MTR_4g120830 | 36.036 | 111 | 62 | 3 | 4 | 107 | 94 | 202 | 2.93e-16 | 71.2 |
| MS.gene84369.t1 | MTR_7g108790 | 36.634 | 101 | 60 | 3 | 8 | 108 | 93 | 189 | 5.70e-15 | 67.4 |
| MS.gene84369.t1 | MTR_3g008760 | 40.000 | 90 | 51 | 2 | 16 | 104 | 136 | 223 | 1.14e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84369.t1 | AT5G62350 | 55.140 | 107 | 46 | 2 | 6 | 110 | 96 | 202 | 7.89e-39 | 129 |
| MS.gene84369.t1 | AT3G47380 | 50.926 | 108 | 51 | 2 | 5 | 110 | 95 | 202 | 1.58e-35 | 120 |
| MS.gene84369.t1 | AT4G25260 | 51.376 | 109 | 51 | 1 | 4 | 110 | 93 | 201 | 3.52e-35 | 119 |
| MS.gene84369.t1 | AT1G62770 | 48.624 | 109 | 54 | 1 | 1 | 107 | 88 | 196 | 2.30e-34 | 117 |
| MS.gene84369.t1 | AT5G62360 | 46.602 | 103 | 53 | 1 | 8 | 110 | 102 | 202 | 5.12e-34 | 117 |
| MS.gene84369.t1 | AT4G12390 | 48.148 | 108 | 53 | 1 | 6 | 110 | 97 | 204 | 3.36e-29 | 104 |
| MS.gene84369.t1 | AT5G20740 | 48.148 | 108 | 52 | 2 | 1 | 105 | 86 | 192 | 1.07e-27 | 100 |
| MS.gene84369.t1 | AT5G20740 | 48.148 | 108 | 52 | 2 | 1 | 105 | 120 | 226 | 2.29e-27 | 100 |
| MS.gene84369.t1 | AT4G00080 | 40.196 | 102 | 56 | 3 | 8 | 105 | 98 | 198 | 1.34e-22 | 87.8 |
| MS.gene84369.t1 | AT4G25250 | 37.624 | 101 | 63 | 0 | 6 | 106 | 96 | 196 | 1.35e-21 | 84.7 |
| MS.gene84369.t1 | AT1G14890 | 41.414 | 99 | 55 | 1 | 14 | 109 | 119 | 217 | 2.99e-21 | 84.3 |
| MS.gene84369.t1 | AT2G01610 | 39.252 | 107 | 52 | 2 | 14 | 109 | 115 | 219 | 2.60e-20 | 82.0 |
| MS.gene84369.t1 | AT1G70720 | 41.237 | 97 | 54 | 2 | 14 | 107 | 110 | 206 | 5.32e-20 | 80.9 |
| MS.gene84369.t1 | AT1G70720 | 41.237 | 97 | 54 | 2 | 14 | 107 | 98 | 194 | 5.93e-20 | 80.5 |
| MS.gene84369.t1 | AT1G62760 | 43.925 | 107 | 53 | 3 | 7 | 110 | 206 | 308 | 6.88e-20 | 82.4 |
| MS.gene84369.t1 | AT5G51520 | 39.796 | 98 | 58 | 1 | 6 | 103 | 103 | 199 | 5.20e-17 | 73.2 |
| MS.gene84369.t1 | AT1G23205 | 36.735 | 98 | 59 | 2 | 10 | 105 | 102 | 198 | 2.70e-16 | 71.2 |
| MS.gene84369.t1 | AT3G14310 | 33.010 | 103 | 68 | 1 | 1 | 103 | 112 | 213 | 2.79e-14 | 67.8 |
| MS.gene84369.t1 | AT3G49220 | 35.644 | 101 | 57 | 2 | 4 | 104 | 187 | 279 | 1.50e-13 | 65.5 |
| MS.gene84369.t1 | AT3G62820 | 35.000 | 100 | 62 | 2 | 11 | 109 | 95 | 192 | 1.98e-13 | 63.5 |
| MS.gene84369.t1 | AT3G49220 | 35.644 | 101 | 57 | 2 | 4 | 104 | 187 | 279 | 2.04e-13 | 65.1 |
| MS.gene84369.t1 | AT3G49220 | 35.644 | 101 | 57 | 2 | 4 | 104 | 142 | 234 | 2.26e-13 | 65.1 |
| MS.gene84369.t1 | AT1G53830 | 33.673 | 98 | 64 | 1 | 6 | 103 | 120 | 216 | 2.93e-12 | 62.0 |
| MS.gene84369.t1 | AT2G47670 | 35.417 | 96 | 60 | 1 | 12 | 107 | 111 | 204 | 1.39e-11 | 58.9 |
| MS.gene84369.t1 | AT5G53370 | 35.000 | 100 | 59 | 3 | 5 | 103 | 166 | 260 | 3.84e-11 | 58.5 |
| MS.gene84369.t1 | AT5G53370 | 35.000 | 100 | 59 | 3 | 5 | 103 | 130 | 224 | 3.93e-11 | 58.5 |
Find 17 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCACTCATTAATCAATA+TGG | 0.298645 | 3.1:-4360770 | MS.gene84369:CDS |
| TAGGCTTAGCCGTTCACTTA+AGG | 0.410233 | 3.1:-4360984 | MS.gene84369:CDS |
| TCAATGGTCAAGATTTCAAT+TGG | 0.421550 | 3.1:-4360943 | MS.gene84369:CDS |
| CGAAGAGCAGCATATTCTCT+TGG | 0.444851 | 3.1:+4361040 | None:intergenic |
| AAATTACTGATAGTGTTGAT+AGG | 0.448838 | 3.1:-4361003 | MS.gene84369:CDS |
| CTTAAACTTTGCAAGATCAA+TGG | 0.453176 | 3.1:-4360959 | MS.gene84369:CDS |
| TTTGGTGGAAAAGCACTTGA+TGG | 0.453873 | 3.1:-4360854 | MS.gene84369:CDS |
| AACTTCTATTAGATCAAGAA+TGG | 0.475846 | 3.1:-4360822 | MS.gene84369:CDS |
| AGTACTTGTAGTGATGGATT+TGG | 0.488408 | 3.1:-4360872 | MS.gene84369:CDS |
| GTTTAAGCTCCTTAAGTGAA+CGG | 0.545857 | 3.1:+4360975 | None:intergenic |
| ACTTGTAGTGATGGATTTGG+TGG | 0.550252 | 3.1:-4360869 | MS.gene84369:CDS |
| TCAATATGGTACTAATCACT+AGG | 0.554200 | 3.1:-4360756 | None:intergenic |
| ATATAAGTAATGTTCAAACA+TGG | 0.577147 | 3.1:-4360919 | MS.gene84369:CDS |
| GATGCAAGTACTTGTAGTGA+TGG | 0.592136 | 3.1:-4360878 | MS.gene84369:CDS |
| CAATATGGTACTAATCACTA+GGG | 0.596788 | 3.1:-4360755 | None:intergenic |
| TATAAGTAATGTTCAAACAT+GGG | 0.606584 | 3.1:-4360918 | MS.gene84369:CDS |
| GGTGGAAAAGCACTTGATGG+AGG | 0.622229 | 3.1:-4360851 | MS.gene84369:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAAGTAATGTTCAAACA+TGG | - | chr3.1:4360905-4360924 | MS.gene84369:CDS | 20.0% |
| !! | TATAAGTAATGTTCAAACAT+GGG | - | chr3.1:4360906-4360925 | MS.gene84369:CDS | 20.0% |
| ! | AACTTCTATTAGATCAAGAA+TGG | - | chr3.1:4361002-4361021 | MS.gene84369:CDS | 25.0% |
| ! | CTTTCACTCATTAATCAATA+TGG | - | chr3.1:4361054-4361073 | MS.gene84369:CDS | 25.0% |
| !!! | AAATTACTGATAGTGTTGAT+AGG | - | chr3.1:4360821-4360840 | MS.gene84369:CDS | 25.0% |
| CTTAAACTTTGCAAGATCAA+TGG | - | chr3.1:4360865-4360884 | MS.gene84369:CDS | 30.0% | |
| TCAATGGTCAAGATTTCAAT+TGG | - | chr3.1:4360881-4360900 | MS.gene84369:CDS | 30.0% | |
| GTTTAAGCTCCTTAAGTGAA+CGG | + | chr3.1:4360852-4360871 | None:intergenic | 35.0% | |
| !! | AGTACTTGTAGTGATGGATT+TGG | - | chr3.1:4360952-4360971 | MS.gene84369:CDS | 35.0% |
| GATGCAAGTACTTGTAGTGA+TGG | - | chr3.1:4360946-4360965 | MS.gene84369:CDS | 40.0% | |
| !! | ACTTGTAGTGATGGATTTGG+TGG | - | chr3.1:4360955-4360974 | MS.gene84369:CDS | 40.0% |
| !! | TTTGGTGGAAAAGCACTTGA+TGG | - | chr3.1:4360970-4360989 | MS.gene84369:CDS | 40.0% |
| CGAAGAGCAGCATATTCTCT+TGG | + | chr3.1:4360787-4360806 | None:intergenic | 45.0% | |
| TAGGCTTAGCCGTTCACTTA+AGG | - | chr3.1:4360840-4360859 | MS.gene84369:CDS | 45.0% | |
| !! | GGTGGAAAAGCACTTGATGG+AGG | - | chr3.1:4360973-4360992 | MS.gene84369:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 4360757 | 4361089 | 4360757 | ID=MS.gene84369 |
| chr3.1 | mRNA | 4360757 | 4361089 | 4360757 | ID=MS.gene84369.t1;Parent=MS.gene84369 |
| chr3.1 | exon | 4360757 | 4361089 | 4360757 | ID=MS.gene84369.t1.exon1;Parent=MS.gene84369.t1 |
| chr3.1 | CDS | 4360757 | 4361089 | 4360757 | ID=cds.MS.gene84369.t1;Parent=MS.gene84369.t1 |
| Gene Sequence |
| Protein sequence |
