Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84612.t1 | AES65081.2 | 81 | 105 | 20 | 0 | 1 | 105 | 31 | 135 | 1.10E-33 | 152.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84612.t1 | G7IMY6 | 81.0 | 105 | 20 | 0 | 1 | 105 | 31 | 135 | 7.7e-34 | 152.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051710 | MS.gene84612 | 0.832548 | 8.88E-56 | -1.69E-46 |
| MS.gene054801 | MS.gene84612 | 0.816477 | 5.42E-52 | -1.69E-46 |
| MS.gene055091 | MS.gene84612 | 0.806294 | 8.82E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene84612 | 0.857479 | 1.59E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84612.t1 | MTR_0125s0060 | 91.000 | 100 | 9 | 0 | 1 | 100 | 31 | 130 | 3.42e-59 | 178 |
| MS.gene84612.t1 | MTR_2g035110 | 80.952 | 105 | 20 | 0 | 1 | 105 | 31 | 135 | 6.54e-53 | 162 |
| MS.gene84612.t1 | MTR_8g066845 | 58.491 | 106 | 24 | 3 | 1 | 105 | 1 | 87 | 5.99e-28 | 97.8 |
| MS.gene84612.t1 | MTR_4g012560 | 70.968 | 62 | 13 | 1 | 18 | 79 | 24 | 80 | 1.06e-18 | 74.3 |
| MS.gene84612.t1 | MTR_2g011670 | 54.000 | 50 | 23 | 0 | 37 | 86 | 18 | 67 | 6.40e-11 | 53.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAGAAGGGGCGCATATT+TGG | 0.259894 | 7.3:+38875672 | MS.gene84612:CDS |
| TGAATTGAAGTGTTCATATT+AGG | 0.299166 | 7.3:-38875897 | None:intergenic |
| CCATTATCACCTTCTCCTTC+CGG | 0.338322 | 7.3:-38875921 | None:intergenic |
| TTCTACCGATGTAGCTTCTT+CGG | 0.360773 | 7.3:+38875749 | MS.gene84612:CDS |
| AAGGGGCGCATATTTGGATT+AGG | 0.373630 | 7.3:+38875678 | MS.gene84612:CDS |
| GATATTTATATGGAAATTGT+TGG | 0.391812 | 7.3:+38875642 | None:intergenic |
| GAGCGCTTGGAGAAAATGAT+TGG | 0.409512 | 7.3:+38875864 | MS.gene84612:CDS |
| CCGGAAGGAGAAGGTGATAA+TGG | 0.416904 | 7.3:+38875921 | MS.gene84612:CDS |
| AGAAACGCTAAAACTGAAGA+TGG | 0.499466 | 7.3:+38875842 | MS.gene84612:CDS |
| AAAACTGAAGATGGAGCGCT+TGG | 0.500382 | 7.3:+38875851 | MS.gene84612:CDS |
| TGTTGGAGGCATTAACAAGA+AGG | 0.515610 | 7.3:+38875659 | MS.gene84612:CDS |
| ATTTATATGGAAATTGTTGG+AGG | 0.522832 | 7.3:+38875645 | None:intergenic |
| TATGAACACTTCAATTCAGC+CGG | 0.533653 | 7.3:+38875902 | MS.gene84612:CDS |
| AGCGCTTGGAGAAAATGATT+GGG | 0.544814 | 7.3:+38875865 | MS.gene84612:CDS |
| TCAATTCAGCCGGAAGGAGA+AGG | 0.553755 | 7.3:+38875912 | MS.gene84612:CDS |
| GTTGGAGGCATTAACAAGAA+GGG | 0.555037 | 7.3:+38875660 | MS.gene84612:CDS |
| GCTACATCGGTAGAAATTGA+AGG | 0.569826 | 7.3:-38875741 | None:intergenic |
| TTGGAGGCATTAACAAGAAG+GGG | 0.580609 | 7.3:+38875661 | MS.gene84612:CDS |
| TCGGTAGAAATTGAAGGCGA+TGG | 0.604104 | 7.3:-38875735 | None:intergenic |
| AACACTTCAATTCAGCCGGA+AGG | 0.621409 | 7.3:+38875906 | MS.gene84612:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTCAAGTTCAAAAGTTAA+TGG | - | chr7.3:38875807-38875826 | None:intergenic | 20.0% |
| !! | TGAATTGAAGTGTTCATATT+AGG | - | chr7.3:38875900-38875919 | None:intergenic | 25.0% |
| AGAAACGCTAAAACTGAAGA+TGG | + | chr7.3:38875842-38875861 | MS.gene84612:CDS | 35.0% | |
| TATGAACACTTCAATTCAGC+CGG | + | chr7.3:38875902-38875921 | MS.gene84612:CDS | 35.0% | |
| ! | TTTTTCCGAAGAAGCTACAT+CGG | - | chr7.3:38875757-38875776 | None:intergenic | 35.0% |
| AGCGCTTGGAGAAAATGATT+GGG | + | chr7.3:38875865-38875884 | MS.gene84612:CDS | 40.0% | |
| GCTACATCGGTAGAAATTGA+AGG | - | chr7.3:38875744-38875763 | None:intergenic | 40.0% | |
| TTCGGAAAAAGTTGCTGCTA+TGG | + | chr7.3:38875767-38875786 | MS.gene84612:CDS | 40.0% | |
| TTCTACCGATGTAGCTTCTT+CGG | + | chr7.3:38875749-38875768 | MS.gene84612:CDS | 40.0% | |
| !! | GTTGGAGGCATTAACAAGAA+GGG | + | chr7.3:38875660-38875679 | MS.gene84612:CDS | 40.0% |
| !! | TGTTGGAGGCATTAACAAGA+AGG | + | chr7.3:38875659-38875678 | MS.gene84612:CDS | 40.0% |
| !! | TTGGAGGCATTAACAAGAAG+GGG | + | chr7.3:38875661-38875680 | MS.gene84612:CDS | 40.0% |
| AACACTTCAATTCAGCCGGA+AGG | + | chr7.3:38875906-38875925 | MS.gene84612:CDS | 45.0% | |
| CCATTATCACCTTCTCCTTC+CGG | - | chr7.3:38875924-38875943 | None:intergenic | 45.0% | |
| GAGCGCTTGGAGAAAATGAT+TGG | + | chr7.3:38875864-38875883 | MS.gene84612:CDS | 45.0% | |
| GGAAAAAGTTGCTGCTATGG+AGG | + | chr7.3:38875770-38875789 | MS.gene84612:CDS | 45.0% | |
| TCGGTAGAAATTGAAGGCGA+TGG | - | chr7.3:38875738-38875757 | None:intergenic | 45.0% | |
| ! | AAAACTGAAGATGGAGCGCT+TGG | + | chr7.3:38875851-38875870 | MS.gene84612:CDS | 45.0% |
| ! | AACAAGAAGGGGCGCATATT+TGG | + | chr7.3:38875672-38875691 | MS.gene84612:CDS | 45.0% |
| ! | AAGGGGCGCATATTTGGATT+AGG | + | chr7.3:38875678-38875697 | MS.gene84612:CDS | 45.0% |
| CCGGAAGGAGAAGGTGATAA+TGG | + | chr7.3:38875921-38875940 | MS.gene84612:CDS | 50.0% | |
| TCAATTCAGCCGGAAGGAGA+AGG | + | chr7.3:38875912-38875931 | MS.gene84612:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 38875651 | 38875968 | 38875651 | ID=MS.gene84612 |
| chr7.3 | mRNA | 38875651 | 38875968 | 38875651 | ID=MS.gene84612.t1;Parent=MS.gene84612 |
| chr7.3 | exon | 38875651 | 38875968 | 38875651 | ID=MS.gene84612.t1.exon1;Parent=MS.gene84612.t1 |
| chr7.3 | CDS | 38875651 | 38875968 | 38875651 | ID=cds.MS.gene84612.t1;Parent=MS.gene84612.t1 |
| Gene Sequence |
| Protein sequence |
