AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85055

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85055.t1 MTR_4g036445 85.624 633 75 3 1 629 1 621 0.0 1100
MS.gene85055.t1 MTR_1g049120 62.343 956 265 17 15 921 8 917 0.0 1064
MS.gene85055.t1 MTR_2g047912 76.271 413 89 3 511 923 275 678 0.0 625
MS.gene85055.t1 MTR_2g047912 56.364 275 76 4 23 263 13 277 3.83e-75 260
MS.gene85055.t1 MTR_1g072780 50.290 690 289 18 260 921 310 973 0.0 594
MS.gene85055.t1 MTR_2g104120 32.800 625 351 18 322 923 258 836 3.79e-82 283
MS.gene85055.t1 MTR_1g066890 39.249 293 130 7 319 611 315 559 1.22e-54 205
MS.gene85055.t1 MTR_1g066890 36.970 165 93 3 760 923 758 912 1.15e-21 101
MS.gene85055.t1 MTR_2g104980 58.252 103 42 1 322 424 233 334 4.08e-36 143
MS.gene85055.t1 MTR_6g005840 35.484 186 116 4 321 505 271 453 9.87e-26 114
MS.gene85055.t1 MTR_6g005840 35.484 186 116 4 321 505 229 411 1.23e-25 114
MS.gene85055.t1 MTR_6g005960 35.484 186 116 4 321 505 211 393 2.11e-24 110
MS.gene85055.t1 MTR_7g113180 33.735 166 95 4 322 479 87 245 9.03e-22 102
MS.gene85055.t1 MTR_2g013200 51.923 104 22 5 63 165 69 145 3.58e-16 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85055.t1 AT5G02950 32.143 616 299 17 325 911 98 623 3.40e-75 259
MS.gene85055.t1 AT5G02950 32.143 616 299 17 325 911 98 623 3.40e-75 259
MS.gene85055.t1 AT3G09670 51.813 193 91 2 321 512 196 387 2.56e-56 208
MS.gene85055.t1 AT3G09670 51.813 193 91 2 321 512 196 387 2.56e-56 208
MS.gene85055.t1 AT3G54760 37.342 316 167 6 604 918 457 742 2.57e-43 169
MS.gene85055.t1 AT3G54760 37.342 316 167 6 604 918 488 773 2.91e-43 169
MS.gene85055.t1 AT5G40340 39.888 178 101 6 322 497 125 298 6.19e-27 119
MS.gene85055.t1 AT5G40340 39.888 178 101 6 322 497 125 298 6.59e-27 119
MS.gene85055.t1 AT5G27650 33.155 187 115 6 322 501 171 354 5.28e-21 99.8
MS.gene85055.t1 AT3G05430 33.333 159 99 3 322 476 133 288 3.14e-20 97.4
MS.gene85055.t1 AT3G05430 33.333 159 99 3 322 476 133 288 3.17e-20 97.1
MS.gene85055.t1 AT3G27860 31.797 217 128 8 293 499 27 233 5.62e-16 83.2
MS.gene85055.t1 AT3G27860 31.797 217 128 8 293 499 27 233 5.62e-16 83.2

Find 193 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCACAAAGTTGTATTTC+TGG 0.189172 4.2:-67526354 None:intergenic
TTCTTTCTCTGATATGGTTT+GGG 0.192247 4.2:+67525807 MS.gene85055:CDS
TGCTATTTCGTTGCGTATTT+TGG 0.192293 4.2:+67525919 MS.gene85055:CDS
ACAAATCCATCGTTTAAAAT+TGG 0.229699 4.2:+67526741 MS.gene85055:CDS
ATCGATGTGAGTGGTGTTTC+TGG 0.239625 4.2:+67525065 MS.gene85055:CDS
AAGATATGAGTGACTCAAAT+TGG 0.253085 4.2:+67527111 MS.gene85055:CDS
GTCTCAAGACGAGTCGAATA+TGG 0.258530 4.2:+67526069 MS.gene85055:CDS
TTTCTTTCTCTGATATGGTT+TGG 0.268068 4.2:+67525806 MS.gene85055:CDS
AACCATATGTTTAGGCGTTT+TGG 0.275299 4.2:+67527401 MS.gene85055:CDS
GGTGATTCTGTTAAGGGTGC+TGG 0.281698 4.2:+67524732 MS.gene85055:CDS
CTCCTTCTCTTGGAATGTTC+AGG 0.288105 4.2:-67526639 None:intergenic
GTCTGTGGAATGTGGGGTTC+AGG 0.296770 4.2:+67524689 MS.gene85055:CDS
GAACAGACCGCAAAGGTTAA+AGG 0.321406 4.2:+67525173 MS.gene85055:CDS
CAATCACCGTCTCGAGAATC+TGG 0.323985 4.2:-67526591 None:intergenic
GCGCGCTACGGCCACGGCCA+CGG 0.337841 4.2:-67524757 None:intergenic
GGGTGCTGGGGCCGTGGCCG+TGG 0.340328 4.2:+67524746 MS.gene85055:CDS
GCAGGTTTGTCGGCAGCTAT+TGG 0.362443 4.2:+67525460 MS.gene85055:CDS
GAAGAAGAATTTAATGAAGC+TGG 0.362869 4.2:+67525667 MS.gene85055:CDS
GGCCGTGGCCGTAGCGCGCC+CGG 0.368754 4.2:+67524761 MS.gene85055:CDS
GACGACTTCGAAATGCAAAC+TGG 0.369537 4.2:+67526681 MS.gene85055:CDS
GAAAGAATGTGTTTCGGAAC+AGG 0.376759 4.2:+67525618 MS.gene85055:CDS
GGGTAGTTTCTTCATCCTCT+TGG 0.377859 4.2:-67527054 None:intergenic
TTCACTATTTGCTGAAATGT+TGG 0.384639 4.2:+67524788 MS.gene85055:CDS
CACATTCCACAGACCCATCT+AGG 0.389260 4.2:-67524680 None:intergenic
CAAACCTTAACCATATGTTT+AGG 0.391917 4.2:+67527393 MS.gene85055:CDS
GTGATTCTGTTAAGGGTGCT+GGG 0.393629 4.2:+67524733 MS.gene85055:CDS
CATATTCTATAAGTTTCATC+GGG 0.394392 4.2:-67526224 None:intergenic
AAGCACAGTCTATAGCATTC+TGG 0.394919 4.2:-67526038 None:intergenic
TTCTGTTGGTGCAGGTTTGT+CGG 0.398560 4.2:+67525450 MS.gene85055:CDS
TATCTTACCTTTAACCTTTG+CGG 0.399233 4.2:-67525180 None:intergenic
CATTTGTGGTGATTCTGTTA+AGG 0.401846 4.2:+67524725 MS.gene85055:CDS
ATTGATGTCACTCCTTCTCT+TGG 0.404257 4.2:-67526649 None:intergenic
ATTTGTGGTGATTCTGTTAA+GGG 0.407472 4.2:+67524726 MS.gene85055:CDS
ATCGTGTCATAGATGTCTTT+AGG 0.408022 4.2:-67526111 None:intergenic
CTGTAATTGTGGCTGCTGAT+TGG 0.409494 4.2:+67527000 MS.gene85055:CDS
ATCCATGGCAGAAGCAGAAA+AGG 0.416193 4.2:+67524599 None:intergenic
AAACTACCCATGGCTGGAAC+TGG 0.419246 4.2:+67527068 MS.gene85055:CDS
TCAGCAAATAGTGAAGTAAC+CGG 0.419526 4.2:-67524780 None:intergenic
TACTGGTGGCTTTGATCGAT+TGG 0.421819 4.2:+67526269 MS.gene85055:CDS
TCAGGTGAAACCAGGTGATC+TGG 0.428952 4.2:-67526621 None:intergenic
GTTAATCGTAGGCCATATTC+CGG 0.429226 4.2:+67527248 MS.gene85055:CDS
GAATCTGGAGTCCCATTCAT+TGG 0.430347 4.2:-67526576 None:intergenic
AACGCCTAAACATATGGTTA+AGG 0.435287 4.2:-67527397 None:intergenic
GGGAAAAGAAAACTAACATA+TGG 0.436804 4.2:+67525688 MS.gene85055:CDS
TGATATTGAACATGCCGATT+GGG 0.449657 4.2:+67524946 MS.gene85055:CDS
GTCCAAAACGCCTAAACATA+TGG 0.451508 4.2:-67527403 None:intergenic
CGATTAACTTGGGCTGGCTC+TGG 0.452688 4.2:-67527233 None:intergenic
AAAGCGTAAATGTAATTTCA+AGG 0.454246 4.2:+67526512 MS.gene85055:CDS
TGTTAAGGGTGCTGGGGCCG+TGG 0.456262 4.2:+67524740 MS.gene85055:CDS
TAGTTCAGAAATAGTTCATC+AGG 0.456384 4.2:+67525744 MS.gene85055:CDS
ATTGGGAATGATAAAGTTGA+TGG 0.457044 4.2:+67524963 MS.gene85055:CDS
TACTATGCATCCAGCAAAGA+AGG 0.457588 4.2:+67526536 MS.gene85055:CDS
TCAACATAATCAACAGTTGT+AGG 0.458971 4.2:-67526852 None:intergenic
GCTTGTAGTTCTGTTGGTGC+AGG 0.459611 4.2:+67525442 MS.gene85055:CDS
AAGAAGAATTTAATGAAGCT+GGG 0.461918 4.2:+67525668 MS.gene85055:CDS
GAAACATCCAATCCATTCTC+AGG 0.469929 4.2:-67526828 None:intergenic
TAGATGGGTCTGTGGAATGT+GGG 0.472616 4.2:+67524682 MS.gene85055:CDS
AAGTCGACAGAGCAAGCAAA+CGG 0.474065 4.2:+67527447 MS.gene85055:CDS
TCAACGTCTCCATCTGTCTT+CGG 0.475054 4.2:-67526798 None:intergenic
TCGGAACAGGTTGGTTCAGA+TGG 0.478265 4.2:+67525631 MS.gene85055:CDS
ACATATTCTATAAGTTTCAT+CGG 0.479992 4.2:-67526225 None:intergenic
ATGGTTAAGGTTTGTTTCTG+AGG 0.480633 4.2:-67527384 None:intergenic
ATTCTCGAGACGGTGATTGT+TGG 0.483757 4.2:+67526595 MS.gene85055:CDS
CATTCTGGAATGATTCTAAA+CGG 0.484135 4.2:-67526023 None:intergenic
GAACAATCTGACGTCCTTGA+GGG 0.485201 4.2:+67525098 MS.gene85055:CDS
TCTCAAGACGAGTCGAATAT+GGG 0.485338 4.2:+67526070 MS.gene85055:CDS
ATTCTTGAAATCAGAGAAGA+AGG 0.488652 4.2:-67526976 None:intergenic
GGCCTACGATTAACTTGGGC+TGG 0.494128 4.2:-67527239 None:intergenic
AACCGGGCGCGCTACGGCCA+CGG 0.497207 4.2:-67524763 None:intergenic
GAATGCTATAGACTGTGCTT+TGG 0.497440 4.2:+67526041 MS.gene85055:CDS
AGAACCTACCCATGGTGGCC+TGG 0.498118 4.2:+67525838 MS.gene85055:CDS
AGTGAAGATAGCATATCATC+TGG 0.502758 4.2:-67526897 None:intergenic
TTGTTGACAATGAACAAGAT+TGG 0.505222 4.2:+67526400 MS.gene85055:CDS
TTGTTGGACTCCAGATCACC+TGG 0.505467 4.2:+67526611 MS.gene85055:CDS
AGGACGTCAGACCCAATGAA+TGG 0.506637 4.2:+67526564 MS.gene85055:CDS
AAAGCTTTGAACACTGCACT+TGG 0.506705 4.2:-67527578 None:intergenic
CATTGTCATTGTCAATGACC+CGG 0.508721 4.2:-67527139 None:intergenic
TGTAGAGAAAGAATGTGTTT+CGG 0.512683 4.2:+67525612 MS.gene85055:CDS
TTGATATTGAACATGCCGAT+TGG 0.513289 4.2:+67524945 MS.gene85055:CDS
TCCCAGTGTTGTCTACAGTC+TGG 0.514788 4.2:-67526140 None:intergenic
AGTCGACAGAGCAAGCAAAC+GGG 0.514954 4.2:+67527448 MS.gene85055:CDS
GAATGAAGCATCTCAGCTAA+AGG 0.515864 4.2:+67525960 MS.gene85055:CDS
GATGTTGGTGAAACCCTAGA+TGG 0.516405 4.2:+67524666 MS.gene85055:CDS
TGATCGTCTGTATTAACTGC+AGG 0.518820 4.2:-67526447 None:intergenic
TCTTTCTCTGATATGGTTTG+GGG 0.522638 4.2:+67525808 MS.gene85055:CDS
ACACTATCTAAGTTGCCTAC+TGG 0.524971 4.2:+67526252 MS.gene85055:CDS
AAGTTCATAACAAGCTCAGC+AGG 0.528013 4.2:-67527341 None:intergenic
GGACGTCAGACCCAATGAAT+GGG 0.528030 4.2:+67526565 MS.gene85055:CDS
GGTTGGTTCAGATGGTGGTC+GGG 0.528631 4.2:+67525639 MS.gene85055:CDS
AATCTGGAGTCCCATTCATT+GGG 0.529750 4.2:-67526575 None:intergenic
TGGTTAAGGTTTGTTTCTGA+GGG 0.530544 4.2:-67527383 None:intergenic
GTCTAATATTGTTGATATCG+TGG 0.534021 4.2:+67524911 MS.gene85055:CDS
AAATTGGTGACTATATTCGT+AGG 0.534916 4.2:+67526757 MS.gene85055:CDS
CACTTGGAGTGTAATTAAGC+TGG 0.535263 4.2:-67527562 None:intergenic
GTGACTCAAATTGGACTGAC+CGG 0.535526 4.2:+67527120 MS.gene85055:CDS
GAGGGATCAAATATCTGTCC+AGG 0.536371 4.2:-67525856 None:intergenic
TTTCAAGAATTCTGTAATTG+TGG 0.537467 4.2:+67526989 MS.gene85055:CDS
ATTGTAGATGAAGTTGTTTG+TGG 0.540117 4.2:+67524996 MS.gene85055:CDS
TTGGTGACAAATCATTTGCA+TGG 0.541342 4.2:+67525938 MS.gene85055:CDS
AGCAAGCAAACGGGCAAGAG+TGG 0.541526 4.2:+67527457 MS.gene85055:CDS
CAGAAAAGTATATTCTCCAA+GGG 0.541983 4.2:-67526940 None:intergenic
TCAGAAAAGTATATTCTCCA+AGG 0.544625 4.2:-67526941 None:intergenic
TTTGCACATGCCGATTGGGG+GGG 0.546602 4.2:+67524827 MS.gene85055:CDS
GTTCATGATGAAGTCATTTG+TGG 0.546872 4.2:+67524711 MS.gene85055:CDS
CTAGGGTTTCACCAACATCA+TGG 0.546967 4.2:-67524662 None:intergenic
TGCCCACTCAGATGCATCTG+AGG 0.546971 4.2:-67525875 None:intergenic
TTCCATCATGCAACAGCACA+TGG 0.548238 4.2:-67525549 None:intergenic
AATATGGCCTACGATTAACT+TGG 0.550585 4.2:-67527244 None:intergenic
TCAACTTTATCATTCCCAAT+CGG 0.551031 4.2:-67524960 None:intergenic
AGTGATGCTTGTAGTTCTGT+TGG 0.553428 4.2:+67525436 MS.gene85055:CDS
CACCTGAACATTCCAAGAGA+AGG 0.554732 4.2:+67526637 MS.gene85055:CDS
ATGAAGAAACTACCCATGGC+TGG 0.555254 4.2:+67527062 MS.gene85055:CDS
GAACAGGTTGGTTCAGATGG+TGG 0.560765 4.2:+67525634 MS.gene85055:CDS
GGAGTCAAATATGAGGCAAA+AGG 0.560804 4.2:+67525588 MS.gene85055:CDS
CTAGATGGGTCTGTGGAATG+TGG 0.562841 4.2:+67524681 MS.gene85055:CDS
TCCATGGCAGAAGCAGAAAA+GGG 0.563521 4.2:+67524600 None:intergenic
TGAACTATTTCTGAACTACC+AGG 0.564289 4.2:-67525739 None:intergenic
ATCAGATCCAGTTCCAGCCA+TGG 0.566118 4.2:-67527075 None:intergenic
TGAAGTAACCGGGCGCGCTA+CGG 0.567068 4.2:-67524769 None:intergenic
TGGAATGTTCAGGTGAAACC+AGG 0.567728 4.2:-67526629 None:intergenic
TAAGAAGTGTGTAGATGCAG+AGG 0.570718 4.2:+67527484 MS.gene85055:CDS
TAGGGTTTCACCAACATCAT+GGG 0.571441 4.2:-67524661 None:intergenic
ATATCTGTCCAGGCCACCAT+GGG 0.571967 4.2:-67525846 None:intergenic
ACGACTTCGAAATGCAAACT+GGG 0.572627 4.2:+67526682 MS.gene85055:CDS
AGGTTGGTTCAGATGGTGGT+CGG 0.574310 4.2:+67525638 MS.gene85055:CDS
TGAACAATCTGACGTCCTTG+AGG 0.574629 4.2:+67525097 MS.gene85055:CDS
ACACTCAAATTACTGAGCAA+TGG 0.575379 4.2:+67525524 MS.gene85055:CDS
ATTCTGTGCACGGATATAGT+TGG 0.576192 4.2:+67527029 MS.gene85055:CDS
ACATTCCACAGACCCATCTA+GGG 0.577354 4.2:-67524679 None:intergenic
CAGAAATAGTTCATCAGGCA+CGG 0.577908 4.2:+67525749 MS.gene85055:CDS
GCCAGACTGTAGACAACACT+GGG 0.580489 4.2:+67526139 MS.gene85055:CDS
AATAAGCATCACACGTGGGG+TGG 0.581145 4.2:+67526173 MS.gene85055:CDS
AGATGGGTCTGTGGAATGTG+GGG 0.582027 4.2:+67524683 MS.gene85055:CDS
ATTGGAGCTTGAGATTGCTA+AGG 0.583274 4.2:+67526287 MS.gene85055:CDS
AATTGGTGACTATATTCGTA+GGG 0.586383 4.2:+67526758 MS.gene85055:CDS
ACCATTGTGGCCCATGATGT+TGG 0.590779 4.2:+67524651 MS.gene85055:CDS
GTTAATACAGACGATCAAGC+TGG 0.592793 4.2:+67526453 MS.gene85055:CDS
AAAGATGCACTTGTGAAGCC+TGG 0.594983 4.2:+67525721 MS.gene85055:CDS
GAATGTGTTTCGGAACAGGT+TGG 0.597469 4.2:+67525622 MS.gene85055:CDS
TGATAACATTGATGATGTTG+AGG 0.597794 4.2:+67524851 MS.gene85055:CDS
AGTTGAAAAGTGCTATTCAA+AGG 0.598334 4.2:+67525025 MS.gene85055:CDS
GAACTCATTCCGAAGACAGA+TGG 0.599666 4.2:+67526789 MS.gene85055:CDS
TCGGCGAGGAGTCAAATATG+AGG 0.599713 4.2:+67525581 MS.gene85055:CDS
GTCGGCAGCTATTGGTAGTG+AGG 0.600055 4.2:+67525468 MS.gene85055:CDS
TCAGATCCAGTTCCAGCCAT+GGG 0.600255 4.2:-67527074 None:intergenic
GCCCACTCAGATGCATCTGA+GGG 0.601078 4.2:-67525874 None:intergenic
TTCTGAAAGCAATCATGCTG+AGG 0.603160 4.2:+67527280 MS.gene85055:CDS
TAGGGCACATTTCTCCACAA+TGG 0.604810 4.2:+67525987 MS.gene85055:CDS
GATAAAGTTGATGGCAAAGG+CGG 0.607171 4.2:+67524972 MS.gene85055:CDS
ACCAACATCATGGGCCACAA+TGG 0.610071 4.2:-67524652 None:intergenic
AACAATCTGACGTCCTTGAG+GGG 0.613239 4.2:+67525099 MS.gene85055:CDS
AGCCATGTGCTGTTGCATGA+TGG 0.616493 4.2:+67525547 MS.gene85055:CDS
GCATTCTCATCTATATAACC+AGG 0.616725 4.2:-67527317 None:intergenic
TCCCTCAGATGCATCTGAGT+GGG 0.618148 4.2:+67525873 MS.gene85055:CDS
CCAGAAATACAACTTTGTGA+AGG 0.619620 4.2:+67526354 MS.gene85055:CDS
AATGATAAAGTTGATGGCAA+AGG 0.621210 4.2:+67524969 MS.gene85055:CDS
AACAGAAAGAAGTAAAACAA+TGG 0.622125 4.2:+67525151 MS.gene85055:CDS
CGGATATAGTTGGCACCAAG+AGG 0.622839 4.2:+67527039 MS.gene85055:CDS
TGACTCAAATTGGACTGACC+GGG 0.622964 4.2:+67527121 MS.gene85055:CDS
CTGTCCAGGCCACCATGGGT+AGG 0.623900 4.2:-67525842 None:intergenic
CTATCTAAGTTGCCTACTGG+TGG 0.624727 4.2:+67526255 MS.gene85055:CDS
CAGCAAATAGTGAAGTAACC+GGG 0.625141 4.2:-67524779 None:intergenic
TTGGAAAGAGATTCTGTGCA+CGG 0.625171 4.2:+67527019 MS.gene85055:CDS
ACTTTGTGAAGGCGTTGACA+AGG 0.626364 4.2:+67526365 MS.gene85055:CDS
ATATGGCCTACGATTAACTT+GGG 0.629330 4.2:-67527243 None:intergenic
AGCCAGACTGTAGACAACAC+TGG 0.630022 4.2:+67526138 MS.gene85055:CDS
CATCTATTAGCATCCCCTCA+AGG 0.633543 4.2:-67525112 None:intergenic
AATCGATCAAAGCCACCAGT+AGG 0.638533 4.2:-67526267 None:intergenic
ATCCCTCAGATGCATCTGAG+TGG 0.638881 4.2:+67525872 MS.gene85055:CDS
GGGACTCCAGATTCTCGAGA+CGG 0.640014 4.2:+67526585 MS.gene85055:CDS
AACAATGGAACAGACCGCAA+AGG 0.641264 4.2:+67525166 MS.gene85055:CDS
AATATCTGTCCAGGCCACCA+TGG 0.643849 4.2:-67525847 None:intergenic
AAGAAATAAGCATCACACGT+GGG 0.646392 4.2:+67526169 MS.gene85055:CDS
AGAAATAAGCATCACACGTG+GGG 0.647299 4.2:+67526170 MS.gene85055:CDS
TGATTCTGTTAAGGGTGCTG+GGG 0.649891 4.2:+67524734 MS.gene85055:CDS
AGAAAAGTATATTCTCCAAG+GGG 0.651247 4.2:-67526939 None:intergenic
TGCAACTTCAGAAACCATTG+TGG 0.658853 4.2:+67524638 MS.gene85055:CDS
AGCCAGCCCAAGTTAATCGT+AGG 0.659455 4.2:+67527237 MS.gene85055:CDS
GGTGGATGAATCTCAAAATG+TGG 0.659940 4.2:+67526191 MS.gene85055:CDS
GCTTGTTATGAACTTTGCCG+AGG 0.662578 4.2:+67527349 MS.gene85055:CDS
TGAGGGAACAGAATTGACCT+CGG 0.667524 4.2:-67527366 None:intergenic
TCTCCACAATGGAAAAGCAG+AGG 0.668805 4.2:+67525998 MS.gene85055:CDS
CAAGAAATAAGCATCACACG+TGG 0.672704 4.2:+67526168 MS.gene85055:CDS
GCAAAGTGAGAACCTACCCA+TGG 0.672895 4.2:+67525830 MS.gene85055:CDS
TTCTCATCTATATAACCAGG+AGG 0.679754 4.2:-67527314 None:intergenic
ATGTTGGTGAAACCCTAGAT+GGG 0.684018 4.2:+67524667 MS.gene85055:CDS
GAGGATGAAGAAACTACCCA+TGG 0.684673 4.2:+67527058 MS.gene85055:CDS
AAGTGAGAACCTACCCATGG+TGG 0.686895 4.2:+67525833 MS.gene85055:CDS
CAATGAACAAGATTGGTGCG+AGG 0.693330 4.2:+67526407 MS.gene85055:CDS
TGAAACCCTAGATGGGTCTG+TGG 0.694469 4.2:+67524674 MS.gene85055:CDS
ATAGCTGACATCGATGTGAG+TGG 0.730677 4.2:+67525056 MS.gene85055:CDS
ATATTCTATAAGTTTCATCG+GGG 0.738354 4.2:-67526223 None:intergenic
ATAGATGTTGAAGATCAACA+AGG 0.760784 4.2:+67525128 MS.gene85055:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTGATTTTTTAAATTTCT+CGG - chr4.2:67526480-67526499 None:intergenic 15.0%
!!! TTTTGTCATCAAAATTTTAT+CGG + chr4.2:67525363-67525382 MS.gene85055:intron 15.0%
!! ACATATTCTATAAGTTTCAT+CGG - chr4.2:67526228-67526247 None:intergenic 20.0%
! AAAGCGTAAATGTAATTTCA+AGG + chr4.2:67526512-67526531 MS.gene85055:CDS 25.0%
! AACAGAAAGAAGTAAAACAA+TGG + chr4.2:67525151-67525170 MS.gene85055:CDS 25.0%
! AAGAAGAATTTAATGAAGCT+GGG + chr4.2:67525668-67525687 MS.gene85055:CDS 25.0%
! ATATTCTATAAGTTTCATCG+GGG - chr4.2:67526226-67526245 None:intergenic 25.0%
! CATATTCTATAAGTTTCATC+GGG - chr4.2:67526227-67526246 None:intergenic 25.0%
! GCTAAAAAGTTCAACATATT+TGG + chr4.2:67527521-67527540 MS.gene85055:CDS 25.0%
! TTTCAAGAATTCTGTAATTG+TGG + chr4.2:67526989-67527008 MS.gene85055:CDS 25.0%
!! AATTACATTTACGCTTTTGA+TGG - chr4.2:67526509-67526528 None:intergenic 25.0%
!! ACAAATCCATCGTTTAAAAT+TGG + chr4.2:67526741-67526760 MS.gene85055:CDS 25.0%
!! ATTACATTTACGCTTTTGAT+GGG - chr4.2:67526508-67526527 None:intergenic 25.0%
!!! AATTTTAAACGATGGATTTG+TGG - chr4.2:67526742-67526761 None:intergenic 25.0%
AAGATATGAGTGACTCAAAT+TGG + chr4.2:67527111-67527130 MS.gene85055:CDS 30.0%
ACTGGGAAAAATACAATTTC+TGG + chr4.2:67526699-67526718 MS.gene85055:CDS 30.0%
ATAGATGTTGAAGATCAACA+AGG + chr4.2:67525128-67525147 MS.gene85055:CDS 30.0%
ATTCTTGAAATCAGAGAAGA+AGG - chr4.2:67526979-67526998 None:intergenic 30.0%
ATTGGGAATGATAAAGTTGA+TGG + chr4.2:67524963-67524982 MS.gene85055:CDS 30.0%
ATTGTAGATGAAGTTGTTTG+TGG + chr4.2:67524996-67525015 MS.gene85055:CDS 30.0%
ATTTGTGGTGATTCTGTTAA+GGG + chr4.2:67524726-67524745 MS.gene85055:CDS 30.0%
CAAACCTTAACCATATGTTT+AGG + chr4.2:67527393-67527412 MS.gene85055:CDS 30.0%
CAGAAAAAAGACAAACTACT+TGG + chr4.2:67527197-67527216 MS.gene85055:CDS 30.0%
GAAGAAGAATTTAATGAAGC+TGG + chr4.2:67525667-67525686 MS.gene85055:CDS 30.0%
GGGAAAAGAAAACTAACATA+TGG + chr4.2:67525688-67525707 MS.gene85055:CDS 30.0%
GTCTAATATTGTTGATATCG+TGG + chr4.2:67524911-67524930 MS.gene85055:CDS 30.0%
TAGTTCAGAAATAGTTCATC+AGG + chr4.2:67525744-67525763 MS.gene85055:CDS 30.0%
TATCTTACCTTTAACCTTTG+CGG - chr4.2:67525183-67525202 None:intergenic 30.0%
TCAACATAATCAACAGTTGT+AGG - chr4.2:67526855-67526874 None:intergenic 30.0%
TCAACTTTATCATTCCCAAT+CGG - chr4.2:67524963-67524982 None:intergenic 30.0%
TGTAGAGAAAGAATGTGTTT+CGG + chr4.2:67525612-67525631 MS.gene85055:CDS 30.0%
TTCAATGTAATCACAAGTGT+CGG - chr4.2:67525343-67525362 None:intergenic 30.0%
TTCTTTCTCTGATATGGTTT+GGG + chr4.2:67525807-67525826 MS.gene85055:CDS 30.0%
TTGTTGACAATGAACAAGAT+TGG + chr4.2:67526400-67526419 MS.gene85055:CDS 30.0%
TTTCTTTCTCTGATATGGTT+TGG + chr4.2:67525806-67525825 MS.gene85055:CDS 30.0%
! AAATTGGTGACTATATTCGT+AGG + chr4.2:67526757-67526776 MS.gene85055:CDS 30.0%
! AATTGGTGACTATATTCGTA+GGG + chr4.2:67526758-67526777 MS.gene85055:CDS 30.0%
! AGAAAAGTATATTCTCCAAG+GGG - chr4.2:67526942-67526961 None:intergenic 30.0%
! ATCTCAGCTAAAGGATTTTA+GGG + chr4.2:67525969-67525988 MS.gene85055:CDS 30.0%
! CAGAAAAGTATATTCTCCAA+GGG - chr4.2:67526943-67526962 None:intergenic 30.0%
! CATGATGGAAAAGAAAAACT+CGG + chr4.2:67525562-67525581 MS.gene85055:CDS 30.0%
! GGAGAATATACTTTTCTGAA+TGG + chr4.2:67526945-67526964 MS.gene85055:CDS 30.0%
! TAGTCACCAATTTTAAACGA+TGG - chr4.2:67526750-67526769 None:intergenic 30.0%
! TCAGAAAAGTATATTCTCCA+AGG - chr4.2:67526944-67526963 None:intergenic 30.0%
! TGAGGAAATGTCAAATTTTG+TGG + chr4.2:67524869-67524888 MS.gene85055:CDS 30.0%
! TTCACTATTTGCTGAAATGT+TGG + chr4.2:67524788-67524807 MS.gene85055:CDS 30.0%
!! AATGATAAAGTTGATGGCAA+AGG + chr4.2:67524969-67524988 MS.gene85055:CDS 30.0%
!! AGTTGAAAAGTGCTATTCAA+AGG + chr4.2:67525025-67525044 MS.gene85055:CDS 30.0%
!! CATTCTGGAATGATTCTAAA+CGG - chr4.2:67526026-67526045 None:intergenic 30.0%
!! TGATAACATTGATGATGTTG+AGG + chr4.2:67524851-67524870 MS.gene85055:CDS 30.0%
!!! CAAGTAGTTTGTCTTTTTTC+TGG - chr4.2:67527199-67527218 None:intergenic 30.0%
!!! GATTGCTTTCAGAACTTTTT+TGG - chr4.2:67527274-67527293 None:intergenic 30.0%
!!! TTTTTCTGGTTTTTCTGCAA+TGG - chr4.2:67527185-67527204 None:intergenic 30.0%
AACGCCTAAACATATGGTTA+AGG - chr4.2:67527400-67527419 None:intergenic 35.0%
AAGAAATAAGCATCACACGT+GGG + chr4.2:67526169-67526188 MS.gene85055:CDS 35.0%
AATATGGCCTACGATTAACT+TGG - chr4.2:67527247-67527266 None:intergenic 35.0%
ACACTCAAATTACTGAGCAA+TGG + chr4.2:67525524-67525543 MS.gene85055:CDS 35.0%
AGTGAAGATAGCATATCATC+TGG - chr4.2:67526900-67526919 None:intergenic 35.0%
ATATGGCCTACGATTAACTT+GGG - chr4.2:67527246-67527265 None:intergenic 35.0%
ATCCAGCAAAGAAGGAAAAA+AGG + chr4.2:67526544-67526563 MS.gene85055:CDS 35.0%
ATCGTGTCATAGATGTCTTT+AGG - chr4.2:67526114-67526133 None:intergenic 35.0%
CATTTGTGGTGATTCTGTTA+AGG + chr4.2:67524725-67524744 MS.gene85055:CDS 35.0%
CCAGAAATACAACTTTGTGA+AGG + chr4.2:67526354-67526373 MS.gene85055:CDS 35.0%
CCTTCACAAAGTTGTATTTC+TGG - chr4.2:67526357-67526376 None:intergenic 35.0%
GCATTCTCATCTATATAACC+AGG - chr4.2:67527320-67527339 None:intergenic 35.0%
GTTCATGATGAAGTCATTTG+TGG + chr4.2:67524711-67524730 MS.gene85055:CDS 35.0%
TCAGCAAATAGTGAAGTAAC+CGG - chr4.2:67524783-67524802 None:intergenic 35.0%
TCTTTCTCTGATATGGTTTG+GGG + chr4.2:67525808-67525827 MS.gene85055:CDS 35.0%
TTCTCATCTATATAACCAGG+AGG - chr4.2:67527317-67527336 None:intergenic 35.0%
TTGATATTGAACATGCCGAT+TGG + chr4.2:67524945-67524964 MS.gene85055:CDS 35.0%
TTTGTTTCCTGCTTAACTAG+TGG + chr4.2:67525223-67525242 MS.gene85055:intron 35.0%
! AACCATATGTTTAGGCGTTT+TGG + chr4.2:67527401-67527420 MS.gene85055:CDS 35.0%
! ATATAACCAGGAGGTTTTTC+AGG - chr4.2:67527308-67527327 None:intergenic 35.0%
! CATCTCAGCTAAAGGATTTT+AGG + chr4.2:67525968-67525987 MS.gene85055:CDS 35.0%
! CGAGTTTTCTTTCTCTGATA+TGG + chr4.2:67525801-67525820 MS.gene85055:CDS 35.0%
! GTTGATGTTTTTCCTGAGAA+TGG + chr4.2:67526816-67526835 MS.gene85055:CDS 35.0%
! TGATATTGAACATGCCGATT+GGG + chr4.2:67524946-67524965 MS.gene85055:CDS 35.0%
! TGTTTTTCCTGAGAATGGAT+TGG + chr4.2:67526821-67526840 MS.gene85055:CDS 35.0%
! TTGGTGACAAATCATTTGCA+TGG + chr4.2:67525938-67525957 MS.gene85055:CDS 35.0%
!! AATTTTATCGGTGTCTTGTC+AGG + chr4.2:67525375-67525394 MS.gene85055:intron 35.0%
!! ATGGTTAAGGTTTGTTTCTG+AGG - chr4.2:67527387-67527406 None:intergenic 35.0%
!! TGAACTATTTCTGAACTACC+AGG - chr4.2:67525742-67525761 None:intergenic 35.0%
!! TGGTTAAGGTTTGTTTCTGA+GGG - chr4.2:67527386-67527405 None:intergenic 35.0%
!!! TGCTATTTCGTTGCGTATTT+TGG + chr4.2:67525919-67525938 MS.gene85055:CDS 35.0%
AAGCACAGTCTATAGCATTC+TGG - chr4.2:67526041-67526060 None:intergenic 40.0%
AAGTTCATAACAAGCTCAGC+AGG - chr4.2:67527344-67527363 None:intergenic 40.0%
ACACTATCTAAGTTGCCTAC+TGG + chr4.2:67526252-67526271 MS.gene85055:CDS 40.0%
ACGACTTCGAAATGCAAACT+GGG + chr4.2:67526682-67526701 MS.gene85055:CDS 40.0%
AGAAATAAGCATCACACGTG+GGG + chr4.2:67526170-67526189 MS.gene85055:CDS 40.0%
ATCTAGAAACCTGTGAAGCA+TGG - chr4.2:67525416-67525435 None:intergenic 40.0%
ATTCTGTGCACGGATATAGT+TGG + chr4.2:67527029-67527048 MS.gene85055:CDS 40.0%
ATTGATGTCACTCCTTCTCT+TGG - chr4.2:67526652-67526671 None:intergenic 40.0%
ATTGGAGCTTGAGATTGCTA+AGG + chr4.2:67526287-67526306 MS.gene85055:CDS 40.0%
CAAGAAATAAGCATCACACG+TGG + chr4.2:67526168-67526187 MS.gene85055:CDS 40.0%
CACTTGGAGTGTAATTAAGC+TGG - chr4.2:67527565-67527584 None:intergenic 40.0%
CAGAAATAGTTCATCAGGCA+CGG + chr4.2:67525749-67525768 MS.gene85055:CDS 40.0%
CAGCAAATAGTGAAGTAACC+GGG - chr4.2:67524782-67524801 None:intergenic 40.0%
CATTGTCATTGTCAATGACC+CGG - chr4.2:67527142-67527161 None:intergenic 40.0%
CTGCCAGCAGTAAAAAAAGA+AGG + chr4.2:67525778-67525797 MS.gene85055:CDS 40.0%
GAAACATCCAATCCATTCTC+AGG - chr4.2:67526831-67526850 None:intergenic 40.0%
GAAAGAATGTGTTTCGGAAC+AGG + chr4.2:67525618-67525637 MS.gene85055:CDS 40.0%
GAATGCTATAGACTGTGCTT+TGG + chr4.2:67526041-67526060 MS.gene85055:CDS 40.0%
GGAGTCAAATATGAGGCAAA+AGG + chr4.2:67525588-67525607 MS.gene85055:CDS 40.0%
GGTGGATGAATCTCAAAATG+TGG + chr4.2:67526191-67526210 MS.gene85055:CDS 40.0%
GTCCAAAACGCCTAAACATA+TGG - chr4.2:67527406-67527425 None:intergenic 40.0%
GTTAATACAGACGATCAAGC+TGG + chr4.2:67526453-67526472 MS.gene85055:CDS 40.0%
GTTAATCGTAGGCCATATTC+CGG + chr4.2:67527248-67527267 MS.gene85055:CDS 40.0%
GTTTCAACCACTAGTTAAGC+AGG - chr4.2:67525233-67525252 None:intergenic 40.0%
TACTATGCATCCAGCAAAGA+AGG + chr4.2:67526536-67526555 MS.gene85055:CDS 40.0%
TAGGGTTTCACCAACATCAT+GGG - chr4.2:67524664-67524683 None:intergenic 40.0%
TCTCAAGACGAGTCGAATAT+GGG + chr4.2:67526070-67526089 MS.gene85055:CDS 40.0%
TGATCGTCTGTATTAACTGC+AGG - chr4.2:67526450-67526469 None:intergenic 40.0%
TGCAACTTCAGAAACCATTG+TGG + chr4.2:67524638-67524657 MS.gene85055:CDS 40.0%
TTGGAAAGAGATTCTGTGCA+CGG + chr4.2:67527019-67527038 MS.gene85055:CDS 40.0%
! AATCTGGAGTCCCATTCATT+GGG - chr4.2:67526578-67526597 None:intergenic 40.0%
! ACGTCTTCGATCAGAAGTAT+TGG - chr4.2:67525304-67525323 None:intergenic 40.0%
! AGTGATGCTTGTAGTTCTGT+TGG + chr4.2:67525436-67525455 MS.gene85055:CDS 40.0%
! ATGTTGGTGAAACCCTAGAT+GGG + chr4.2:67524667-67524686 MS.gene85055:CDS 40.0%
! GAATGAAGCATCTCAGCTAA+AGG + chr4.2:67525960-67525979 MS.gene85055:CDS 40.0%
! GGTTTTTCTGCAATGGATGT+TGG - chr4.2:67527178-67527197 None:intergenic 40.0%
! TAAGAAGTGTGTAGATGCAG+AGG + chr4.2:67527484-67527503 MS.gene85055:CDS 40.0%
! TTCTGAAAGCAATCATGCTG+AGG + chr4.2:67527280-67527299 MS.gene85055:CDS 40.0%
!! AAAGCTTTGAACACTGCACT+TGG - chr4.2:67527581-67527600 None:intergenic 40.0%
!! GATAAAGTTGATGGCAAAGG+CGG + chr4.2:67524972-67524991 MS.gene85055:CDS 40.0%
!!! GAACTTTTTTGGCCGGAATA+TGG - chr4.2:67527263-67527282 None:intergenic 40.0%
!!! GCTTTCAGAACTTTTTTGGC+CGG - chr4.2:67527270-67527289 None:intergenic 40.0%
!!! GTCCTTTTTTCCTTCTTTGC+TGG - chr4.2:67526549-67526568 None:intergenic 40.0%
!!! TCGCCTTCTTTTTTTACTGC+TGG - chr4.2:67525784-67525803 None:intergenic 40.0%
AAACCTGTGAAGCATGGACA+CGG - chr4.2:67525410-67525429 None:intergenic 45.0%
AAAGATGCACTTGTGAAGCC+TGG + chr4.2:67525721-67525740 MS.gene85055:CDS 45.0%
AACAATCTGACGTCCTTGAG+GGG + chr4.2:67525099-67525118 MS.gene85055:CDS 45.0%
AACAATGGAACAGACCGCAA+AGG + chr4.2:67525166-67525185 MS.gene85055:CDS 45.0%
AAGTCGACAGAGCAAGCAAA+CGG + chr4.2:67527447-67527466 MS.gene85055:CDS 45.0%
AATCGATCAAAGCCACCAGT+AGG - chr4.2:67526270-67526289 None:intergenic 45.0%
ACATTCCACAGACCCATCTA+GGG - chr4.2:67524682-67524701 None:intergenic 45.0%
ATAGCTGACATCGATGTGAG+TGG + chr4.2:67525056-67525075 MS.gene85055:CDS 45.0%
ATCGATGTGAGTGGTGTTTC+TGG + chr4.2:67525065-67525084 MS.gene85055:CDS 45.0%
ATGAAGAAACTACCCATGGC+TGG + chr4.2:67527062-67527081 MS.gene85055:CDS 45.0%
ATGTTATCATCCCCCCCAAT+CGG - chr4.2:67524840-67524859 None:intergenic 45.0%
ATTCTCGAGACGGTGATTGT+TGG + chr4.2:67526595-67526614 MS.gene85055:CDS 45.0%
CAATGAACAAGATTGGTGCG+AGG + chr4.2:67526407-67526426 MS.gene85055:CDS 45.0%
CACCTGAACATTCCAAGAGA+AGG + chr4.2:67526637-67526656 MS.gene85055:CDS 45.0%
CATCTATTAGCATCCCCTCA+AGG - chr4.2:67525115-67525134 None:intergenic 45.0%
CTAGGGTTTCACCAACATCA+TGG - chr4.2:67524665-67524684 None:intergenic 45.0%
CTATCTAAGTTGCCTACTGG+TGG + chr4.2:67526255-67526274 MS.gene85055:CDS 45.0%
CTCCTTCTCTTGGAATGTTC+AGG - chr4.2:67526642-67526661 None:intergenic 45.0%
CTGTAATTGTGGCTGCTGAT+TGG + chr4.2:67527000-67527019 MS.gene85055:CDS 45.0%
GAACAATCTGACGTCCTTGA+GGG + chr4.2:67525098-67525117 MS.gene85055:CDS 45.0%
GAACAGACCGCAAAGGTTAA+AGG + chr4.2:67525173-67525192 MS.gene85055:CDS 45.0%
GAACTCATTCCGAAGACAGA+TGG + chr4.2:67526789-67526808 MS.gene85055:CDS 45.0%
GAATGTGTTTCGGAACAGGT+TGG + chr4.2:67525622-67525641 MS.gene85055:CDS 45.0%
GACGACTTCGAAATGCAAAC+TGG + chr4.2:67526681-67526700 MS.gene85055:CDS 45.0%
GAGGATGAAGAAACTACCCA+TGG + chr4.2:67527058-67527077 MS.gene85055:CDS 45.0%
GAGGGATCAAATATCTGTCC+AGG - chr4.2:67525859-67525878 None:intergenic 45.0%
GGGTAGTTTCTTCATCCTCT+TGG - chr4.2:67527057-67527076 None:intergenic 45.0%
GTCTCAAGACGAGTCGAATA+TGG + chr4.2:67526069-67526088 MS.gene85055:CDS 45.0%
GTGACTCAAATTGGACTGAC+CGG + chr4.2:67527120-67527139 MS.gene85055:CDS 45.0%
TAGATGGGTCTGTGGAATGT+GGG + chr4.2:67524682-67524701 MS.gene85055:CDS 45.0%
TAGGGCACATTTCTCCACAA+TGG + chr4.2:67525987-67526006 MS.gene85055:CDS 45.0%
TCAACGTCTCCATCTGTCTT+CGG - chr4.2:67526801-67526820 None:intergenic 45.0%
TCTCCACAATGGAAAAGCAG+AGG + chr4.2:67525998-67526017 MS.gene85055:CDS 45.0%
TGAACAATCTGACGTCCTTG+AGG + chr4.2:67525097-67525116 MS.gene85055:CDS 45.0%
TGACTCAAATTGGACTGACC+GGG + chr4.2:67527121-67527140 MS.gene85055:CDS 45.0%
TGAGGGAACAGAATTGACCT+CGG - chr4.2:67527369-67527388 None:intergenic 45.0%
TGGAAAAGAAAAACTCGGCG+AGG + chr4.2:67525567-67525586 MS.gene85055:CDS 45.0%
TGGAATGTTCAGGTGAAACC+AGG - chr4.2:67526632-67526651 None:intergenic 45.0%
TGTGTTGCACAAAAACCCCT+TGG + chr4.2:67526924-67526943 MS.gene85055:CDS 45.0%
TTCCATCATGCAACAGCACA+TGG - chr4.2:67525552-67525571 None:intergenic 45.0%
TTCTGTTGGTGCAGGTTTGT+CGG + chr4.2:67525450-67525469 MS.gene85055:CDS 45.0%
! CTCAGCTGCTTGCGTTTTAT+CGG + chr4.2:67526310-67526329 MS.gene85055:CDS 45.0%
! GAATCTGGAGTCCCATTCAT+TGG - chr4.2:67526579-67526598 None:intergenic 45.0%
! GATGTTGGTGAAACCCTAGA+TGG + chr4.2:67524666-67524685 MS.gene85055:CDS 45.0%
! TCGATTTTGCACATGCCGAT+TGG + chr4.2:67524822-67524841 MS.gene85055:CDS 45.0%
!! ACTTTGTGAAGGCGTTGACA+AGG + chr4.2:67526365-67526384 MS.gene85055:CDS 45.0%
!! ATTTTGCACATGCCGATTGG+GGG + chr4.2:67524825-67524844 MS.gene85055:CDS 45.0%
!! CGATTTTGCACATGCCGATT+GGG + chr4.2:67524823-67524842 MS.gene85055:CDS 45.0%
!! GATTTTGCACATGCCGATTG+GGG + chr4.2:67524824-67524843 MS.gene85055:CDS 45.0%
!! GCTTGTTATGAACTTTGCCG+AGG + chr4.2:67527349-67527368 MS.gene85055:CDS 45.0%
!! GTAATCACAAGTGTCGGTGT+CGG - chr4.2:67525337-67525356 None:intergenic 45.0%
!! GTGATTCTGTTAAGGGTGCT+GGG + chr4.2:67524733-67524752 MS.gene85055:CDS 45.0%
!! TACTGGTGGCTTTGATCGAT+TGG + chr4.2:67526269-67526288 MS.gene85055:CDS 45.0%
!! TAGGCGTTTTGGACGACTAA+AGG + chr4.2:67527412-67527431 MS.gene85055:CDS 45.0%
!! TGATTCTGTTAAGGGTGCTG+GGG + chr4.2:67524734-67524753 MS.gene85055:CDS 45.0%
AAACTACCCATGGCTGGAAC+TGG + chr4.2:67527068-67527087 MS.gene85055:CDS 50.0%
AAGTGAGAACCTACCCATGG+TGG + chr4.2:67525833-67525852 MS.gene85055:CDS 50.0%
AATAAGCATCACACGTGGGG+TGG + chr4.2:67526173-67526192 MS.gene85055:CDS 50.0%
AATATCTGTCCAGGCCACCA+TGG - chr4.2:67525850-67525869 None:intergenic 50.0%
ACCAACATCATGGGCCACAA+TGG - chr4.2:67524655-67524674 None:intergenic 50.0%
ACCATTGTGGCCCATGATGT+TGG + chr4.2:67524651-67524670 MS.gene85055:CDS 50.0%
AGATGGGTCTGTGGAATGTG+GGG + chr4.2:67524683-67524702 MS.gene85055:CDS 50.0%
AGCCAGACTGTAGACAACAC+TGG + chr4.2:67526138-67526157 MS.gene85055:CDS 50.0%
AGCCAGCCCAAGTTAATCGT+AGG + chr4.2:67527237-67527256 MS.gene85055:CDS 50.0%
AGGACGTCAGACCCAATGAA+TGG + chr4.2:67526564-67526583 MS.gene85055:CDS 50.0%
AGTCGACAGAGCAAGCAAAC+GGG + chr4.2:67527448-67527467 MS.gene85055:CDS 50.0%
ATATCTGTCCAGGCCACCAT+GGG - chr4.2:67525849-67525868 None:intergenic 50.0%
ATCAGATCCAGTTCCAGCCA+TGG - chr4.2:67527078-67527097 None:intergenic 50.0%
ATCCCTCAGATGCATCTGAG+TGG + chr4.2:67525872-67525891 MS.gene85055:CDS 50.0%
CAATCACCGTCTCGAGAATC+TGG - chr4.2:67526594-67526613 None:intergenic 50.0%
CACATTCCACAGACCCATCT+AGG - chr4.2:67524683-67524702 None:intergenic 50.0%
CGGATATAGTTGGCACCAAG+AGG + chr4.2:67527039-67527058 MS.gene85055:CDS 50.0%
CTAGATGGGTCTGTGGAATG+TGG + chr4.2:67524681-67524700 MS.gene85055:CDS 50.0%
GCAAAGTGAGAACCTACCCA+TGG + chr4.2:67525830-67525849 MS.gene85055:CDS 50.0%
GCCAGACTGTAGACAACACT+GGG + chr4.2:67526139-67526158 MS.gene85055:CDS 50.0%
GGACGTCAGACCCAATGAAT+GGG + chr4.2:67526565-67526584 MS.gene85055:CDS 50.0%
TCAGATCCAGTTCCAGCCAT+GGG - chr4.2:67527077-67527096 None:intergenic 50.0%
TCAGGTGAAACCAGGTGATC+TGG - chr4.2:67526624-67526643 None:intergenic 50.0%
TCCCAGTGTTGTCTACAGTC+TGG - chr4.2:67526143-67526162 None:intergenic 50.0%
TCCCTCAGATGCATCTGAGT+GGG + chr4.2:67525873-67525892 MS.gene85055:CDS 50.0%
TCGGCGAGGAGTCAAATATG+AGG + chr4.2:67525581-67525600 MS.gene85055:CDS 50.0%
TGAAACCCTAGATGGGTCTG+TGG + chr4.2:67524674-67524693 MS.gene85055:CDS 50.0%
TTGTTGGACTCCAGATCACC+TGG + chr4.2:67526611-67526630 MS.gene85055:CDS 50.0%
! AGCCATGTGCTGTTGCATGA+TGG + chr4.2:67525547-67525566 MS.gene85055:CDS 50.0%
! AGGTTGGTTCAGATGGTGGT+CGG + chr4.2:67525638-67525657 MS.gene85055:CDS 50.0%
! GCTTGTAGTTCTGTTGGTGC+AGG + chr4.2:67525442-67525461 MS.gene85055:CDS 50.0%
! GGTGATTCTGTTAAGGGTGC+TGG + chr4.2:67524732-67524751 MS.gene85055:CDS 50.0%
!! GAACAGGTTGGTTCAGATGG+TGG + chr4.2:67525634-67525653 MS.gene85055:CDS 50.0%
!! GCTCCTCTGCTTTTCCATTG+TGG - chr4.2:67526004-67526023 None:intergenic 50.0%
!! TCGGAACAGGTTGGTTCAGA+TGG + chr4.2:67525631-67525650 MS.gene85055:CDS 50.0%
!! TTTTGCACATGCCGATTGGG+GGG + chr4.2:67524826-67524845 MS.gene85055:CDS 50.0%
AGCAAGCAAACGGGCAAGAG+TGG + chr4.2:67527457-67527476 MS.gene85055:CDS 55.0%
GAGGCTCCTGAAAAACCTCC+TGG + chr4.2:67527299-67527318 MS.gene85055:CDS 55.0%
GCAGGTTTGTCGGCAGCTAT+TGG + chr4.2:67525460-67525479 MS.gene85055:CDS 55.0%
GCCCACTCAGATGCATCTGA+GGG - chr4.2:67525877-67525896 None:intergenic 55.0%
GGGACTCCAGATTCTCGAGA+CGG + chr4.2:67526585-67526604 MS.gene85055:CDS 55.0%
GTCTGTGGAATGTGGGGTTC+AGG + chr4.2:67524689-67524708 MS.gene85055:CDS 55.0%
TGCCCACTCAGATGCATCTG+AGG - chr4.2:67525878-67525897 None:intergenic 55.0%
TGTCCGTGTCCATGCTTCAC+AGG + chr4.2:67525404-67525423 MS.gene85055:intron 55.0%
! GGCCTACGATTAACTTGGGC+TGG - chr4.2:67527242-67527261 None:intergenic 55.0%
! GGTTGGTTCAGATGGTGGTC+GGG + chr4.2:67525639-67525658 MS.gene85055:CDS 55.0%
! TTTGCACATGCCGATTGGGG+GGG + chr4.2:67524827-67524846 MS.gene85055:CDS 55.0%
!! CGATTAACTTGGGCTGGCTC+TGG - chr4.2:67527236-67527255 None:intergenic 55.0%
!! GTCGGCAGCTATTGGTAGTG+AGG + chr4.2:67525468-67525487 MS.gene85055:CDS 55.0%
AGAACCTACCCATGGTGGCC+TGG + chr4.2:67525838-67525857 MS.gene85055:CDS 60.0%
TGAAGTAACCGGGCGCGCTA+CGG - chr4.2:67524772-67524791 None:intergenic 60.0%
!! AAGTGTCGGTGTCGGACGCT+GGG - chr4.2:67525329-67525348 None:intergenic 60.0%
CTGTCCAGGCCACCATGGGT+AGG - chr4.2:67525845-67525864 None:intergenic 65.0%
!! CAAGTGTCGGTGTCGGACGC+TGG - chr4.2:67525330-67525349 None:intergenic 65.0%
!! TGTTAAGGGTGCTGGGGCCG+TGG + chr4.2:67524740-67524759 MS.gene85055:CDS 65.0%
AACCGGGCGCGCTACGGCCA+CGG - chr4.2:67524766-67524785 None:intergenic 75.0%
! GCGCGCTACGGCCACGGCCA+CGG - chr4.2:67524760-67524779 None:intergenic 80.0%
! GGCCGTGGCCGTAGCGCGCC+CGG + chr4.2:67524761-67524780 MS.gene85055:CDS 85.0%
!!! GGGTGCTGGGGCCGTGGCCG+TGG + chr4.2:67524746-67524765 MS.gene85055:CDS 85.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 67524603 67527619 67524603 ID=MS.gene85055
chr4.2 mRNA 67524603 67527619 67524603 ID=MS.gene85055.t1;Parent=MS.gene85055
chr4.2 exon 67524603 67525194 67524603 ID=MS.gene85055.t1.exon1;Parent=MS.gene85055.t1
chr4.2 CDS 67524603 67525194 67524603 ID=cds.MS.gene85055.t1;Parent=MS.gene85055.t1
chr4.2 exon 67525434 67527619 67525434 ID=MS.gene85055.t1.exon2;Parent=MS.gene85055.t1
chr4.2 CDS 67525434 67527619 67525434 ID=cds.MS.gene85055.t1;Parent=MS.gene85055.t1
Gene Sequence

>MS.gene85055

ATGGCAGAAGCAGAAAAGGGTTTGTTGTTTACTTCTGCAACTTCAGAAACCATTGTGGCCCATGATGTTGGTGAAACCCTAGATGGGTCTGTGGAATGTGGGGTTCAGGTTCATGATGAAGTCATTTGTGGTGATTCTGTTAAGGGTGCTGGGGCCGTGGCCGTGGCCGTAGCGCGCCCGGTTACTTCACTATTTGCTGAAATGTTGGAAACTATTGATTCGATTTTGCACATGCCGATTGGGGGGGATGATAACATTGATGATGTTGAGGAAATGTCAAATTTTGTGGATGATAAATTTGACGCGAAGTCTAATATTGTTGATATCGTGGAAACGATAGATTTGATATTGAACATGCCGATTGGGAATGATAAAGTTGATGGCAAAGGCGGTATTGTAGATGAAGTTGTTTGTGGAACTGAAGTTGAAAAGTGCTATTCAAAGGAGCAAATAATAGCTGACATCGATGTGAGTGGTGTTTCTGGTTTGAATGCTGAACAATCTGACGTCCTTGAGGGGATGCTAATAGATGTTGAAGATCAACAAGGAACAGAAAGAAGTAAAACAATGGAACAGACCGCAAAGGTTAAAGGTAAGATATCTGCTGATTTTATTGATGTTTTGTTTCCTGCTTAACTAGTGGTTGAAACTGTAATTGTTGTAAATGAGAATGTTGTTTAGTTTCTAGCTTTGTTGCACCAATACTTCTGATCGAAGACGTGTCCCAGCGTCCGACACCGACACTTGTGATTACATTGAATTTTGTCATCAAAATTTTATCGGTGTCTTGTCAGGTGTTTGTGTCCGTGTCCATGCTTCACAGGTTTCTAGATAGTGATGCTTGTAGTTCTGTTGGTGCAGGTTTGTCGGCAGCTATTGGTAGTGAGGAAATTTTTGATGTTAATGCAATTGTAGAAAAAGACACTCAAATTACTGAGCAATGGAGCCATGTGCTGTTGCATGATGGAAAAGAAAAACTCGGCGAGGAGTCAAATATGAGGCAAAAGGTTGTAGAGAAAGAATGTGTTTCGGAACAGGTTGGTTCAGATGGTGGTCGGGAAATTGAAGAAGAATTTAATGAAGCTGGGAAAAGAAAACTAACATATGGAAAAGCTGTAAAAGATGCACTTGTGAAGCCTGGTAGTTCAGAAATAGTTCATCAGGCACGGTATTCGCTGCCAGCAGTAAAAAAAGAAGGCGAGTTTTCTTTCTCTGATATGGTTTGGGGCAAAGTGAGAACCTACCCATGGTGGCCTGGACAGATATTTGATCCCTCAGATGCATCTGAGTGGGCAATGAAGCATTATAAAAAAGATTGCTATTTCGTTGCGTATTTTGGTGACAAATCATTTGCATGGAATGAAGCATCTCAGCTAAAGGATTTTAGGGCACATTTCTCCACAATGGAAAAGCAGAGGAGCCGTTTAGAATCATTCCAGAATGCTATAGACTGTGCTTTGGATGAAGTCTCAAGACGAGTCGAATATGGGCTAGCATGTTCTTGTATACCTAAAGACATCTATGACACGATCAAAAGCCAGACTGTAGACAACACTGGGATTCGACAAGAAATAAGCATCACACGTGGGGTGGATGAATCTCAAAATGTGGACTCATTTTCCCCGATGAAACTTATAGAATATGTGAAAACACTATCTAAGTTGCCTACTGGTGGCTTTGATCGATTGGAGCTTGAGATTGCTAAGGCTCAGCTGCTTGCGTTTTATCGGTTTAAGAGTTTCTCTTGCTTGCCAGAAATACAACTTTGTGAAGGCGTTGACAAGGACAATTTATTTTTTGTTGACAATGAACAAGATTGGTGCGAGGTTATTGAGCATGCAACTCCTGCAGTTAATACAGACGATCAAGCTGGTCCGAGAAATTTAAAAAATCAAAGCAGCACCCATCAAAAGCGTAAATGTAATTTCAAGGATACTATGCATCCAGCAAAGAAGGAAAAAAGGACGTCAGACCCAATGAATGGGACTCCAGATTCTCGAGACGGTGATTGTTGGACTCCAGATCACCTGGTTTCACCTGAACATTCCAAGAGAAGGAGTGACATCAATCACTTTGATGACGACTTCGAAATGCAAACTGGGAAAAATACAATTTCTGGAACAAAAGTTTCAAATACCACAAATCCATCGTTTAAAATTGGTGACTATATTCGTAGGGCTGCTAGCGAACTCATTCCGAAGACAGATGGAGACGTTGATGTTTTTCCTGAGAATGGATTGGATGTTTCTCCTACAACTGTTGATTATGTTGAGAACACGACTGAATATTCGTCTCCAGATGATATGCTATCTTCACTTCAGTGTGTTGCACAAAAACCCCTTGGAGAATATACTTTTCTGAATGGCATAGTCACCTTCTTCTCTGATTTCAAGAATTCTGTAATTGTGGCTGCTGATTGGAAAGAGATTCTGTGCACGGATATAGTTGGCACCAAGAGGATGAAGAAACTACCCATGGCTGGAACTGGATCTGATACATTTGAATTTGAAGATATGAGTGACTCAAATTGGACTGACCGGGTCATTGACAATGACAATGAAAAGAAATCAGTCCAACATCCATTGCAGAAAAACCAGAAAAAAGACAAACTACTTGGCGCTGCTAAGTCACCAGAGCCAGCCCAAGTTAATCGTAGGCCATATTCCGGCCAAAAAAGTTCTGAAAGCAATCATGCTGAGGCTCCTGAAAAACCTCCTGGTTATATAGATGAGAATGCTCCTGCTGAGCTTGTTATGAACTTTGCCGAGGTCAATTCTGTTCCCTCAGAAACAAACCTTAACCATATGTTTAGGCGTTTTGGACGACTAAAGGAATCTGAAACAGAAGTCGACAGAGCAAGCAAACGGGCAAGAGTGGTCTTTAAGAAGTGTGTAGATGCAGAGGTAGCTCTCAGTAGTGCTAAAAAGTTCAACATATTTGGCTCAGTACTCGTAGATTACCAGCTTAATTACACTCCAAGTGCAGTGTTCAAAGCTTTTAGCAAAAAACATGATTAG
Protein sequence

>MS.gene85055.t1

MAEAEKGLLFTSATSETIVAHDVGETLDGSVECGVQVHDEVICGDSVKGAGAVAVAVARPVTSLFAEMLETIDSILHMPIGGDDNIDDVEEMSNFVDDKFDAKSNIVDIVETIDLILNMPIGNDKVDGKGGIVDEVVCGTEVEKCYSKEQIIADIDVSGVSGLNAEQSDVLEGMLIDVEDQQGTERSKTMEQTAKVKDSDACSSVGAGLSAAIGSEEIFDVNAIVEKDTQITEQWSHVLLHDGKEKLGEESNMRQKVVEKECVSEQVGSDGGREIEEEFNEAGKRKLTYGKAVKDALVKPGSSEIVHQARYSLPAVKKEGEFSFSDMVWGKVRTYPWWPGQIFDPSDASEWAMKHYKKDCYFVAYFGDKSFAWNEASQLKDFRAHFSTMEKQRSRLESFQNAIDCALDEVSRRVEYGLACSCIPKDIYDTIKSQTVDNTGIRQEISITRGVDESQNVDSFSPMKLIEYVKTLSKLPTGGFDRLELEIAKAQLLAFYRFKSFSCLPEIQLCEGVDKDNLFFVDNEQDWCEVIEHATPAVNTDDQAGPRNLKNQSSTHQKRKCNFKDTMHPAKKEKRTSDPMNGTPDSRDGDCWTPDHLVSPEHSKRRSDINHFDDDFEMQTGKNTISGTKVSNTTNPSFKIGDYIRRAASELIPKTDGDVDVFPENGLDVSPTTVDYVENTTEYSSPDDMLSSLQCVAQKPLGEYTFLNGIVTFFSDFKNSVIVAADWKEILCTDIVGTKRMKKLPMAGTGSDTFEFEDMSDSNWTDRVIDNDNEKKSVQHPLQKNQKKDKLLGAAKSPEPAQVNRRPYSGQKSSESNHAEAPEKPPGYIDENAPAELVMNFAEVNSVPSETNLNHMFRRFGRLKESETEVDRASKRARVVFKKCVDAEVALSSAKKFNIFGSVLVDYQLNYTPSAVFKAFSKKHD