Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85284.t1 | XP_024632573.1 | 98.3 | 949 | 16 | 0 | 1 | 949 | 1 | 949 | 0.00E+00 | 1791.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85284.t1 | Q6C8F7 | 19.5 | 400 | 272 | 12 | 29 | 394 | 6 | 389 | 3.0e-06 | 55.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85284.t1 | A0A072VMX4 | 98.3 | 949 | 16 | 0 | 1 | 949 | 1 | 949 | 0.0e+00 | 1791.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene54899 | MS.gene85284 | PPI |
| MS.gene025598 | MS.gene85284 | PPI |
| MS.gene68267 | MS.gene85284 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85284.t1 | MTR_2g098850 | 98.314 | 949 | 16 | 0 | 1 | 949 | 1 | 949 | 0.0 | 1923 |
| MS.gene85284.t1 | MTR_2g098850 | 96.285 | 969 | 16 | 1 | 1 | 949 | 1 | 969 | 0.0 | 1910 |
| MS.gene85284.t1 | MTR_4g067110 | 70.213 | 987 | 242 | 5 | 1 | 949 | 1 | 973 | 0.0 | 1382 |
| MS.gene85284.t1 | MTR_7g091780 | 44.214 | 1011 | 475 | 16 | 2 | 949 | 6 | 990 | 0.0 | 801 |
| MS.gene85284.t1 | MTR_4g039320 | 55.598 | 518 | 212 | 2 | 1 | 518 | 1 | 500 | 0.0 | 624 |
| MS.gene85284.t1 | MTR_4g039320 | 38.369 | 417 | 228 | 10 | 554 | 949 | 620 | 1028 | 7.96e-62 | 228 |
| MS.gene85284.t1 | MTR_1g084090 | 33.784 | 1036 | 560 | 25 | 1 | 949 | 1 | 997 | 1.00e-177 | 542 |
| MS.gene85284.t1 | MTR_1g082810 | 52.273 | 88 | 42 | 0 | 50 | 137 | 12 | 99 | 8.72e-24 | 97.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85284.t1 | AT1G05960 | 55.075 | 995 | 388 | 13 | 1 | 949 | 1 | 982 | 0.0 | 1035 |
| MS.gene85284.t1 | AT1G05960 | 55.075 | 995 | 388 | 13 | 1 | 949 | 1 | 982 | 0.0 | 1035 |
| MS.gene85284.t1 | AT1G05960 | 55.075 | 995 | 388 | 13 | 1 | 949 | 1 | 982 | 0.0 | 1035 |
| MS.gene85284.t1 | AT1G05960 | 54.035 | 1016 | 387 | 14 | 1 | 949 | 1 | 1003 | 0.0 | 1023 |
| MS.gene85284.t1 | AT2G41830 | 52.941 | 527 | 231 | 3 | 2 | 518 | 6 | 525 | 0.0 | 604 |
| MS.gene85284.t1 | AT2G41830 | 38.499 | 413 | 232 | 7 | 551 | 949 | 621 | 1025 | 1.82e-66 | 242 |
| MS.gene85284.t1 | AT5G21080 | 55.058 | 514 | 217 | 3 | 1 | 507 | 1 | 507 | 0.0 | 592 |
| MS.gene85284.t1 | AT5G21080 | 40.000 | 365 | 172 | 14 | 542 | 883 | 624 | 964 | 2.65e-50 | 193 |
| MS.gene85284.t1 | AT5G26850 | 35.010 | 1014 | 559 | 23 | 1 | 948 | 1 | 980 | 0.0 | 555 |
| MS.gene85284.t1 | AT5G26850 | 35.010 | 1014 | 559 | 23 | 1 | 948 | 1 | 980 | 0.0 | 555 |
| MS.gene85284.t1 | AT5G26850 | 35.010 | 1014 | 559 | 23 | 1 | 948 | 1 | 980 | 0.0 | 555 |
| MS.gene85284.t1 | AT5G26850 | 35.010 | 1014 | 559 | 23 | 1 | 948 | 1 | 980 | 0.0 | 555 |
| MS.gene85284.t1 | AT2G41830 | 48.111 | 397 | 189 | 3 | 132 | 518 | 1 | 390 | 1.17e-125 | 402 |
| MS.gene85284.t1 | AT2G41830 | 38.499 | 413 | 232 | 7 | 551 | 949 | 486 | 890 | 5.01e-67 | 242 |
Find 204 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGATCTCATTAAACATTTA+AGG | 0.108763 | 2.4:+5170741 | MS.gene85284:CDS |
| ATAGATTATATCAGCAATTT+TGG | 0.141544 | 2.4:+5170024 | MS.gene85284:intron |
| GTCTCTTGCTTTCTTCAATC+TGG | 0.151994 | 2.4:+5172045 | MS.gene85284:CDS |
| GTCAGGGATGCTTTGACTTT+TGG | 0.153104 | 2.4:-5172887 | None:intergenic |
| CGTTGCTTCCTTTGCCAATT+TGG | 0.154788 | 2.4:-5170349 | None:intergenic |
| AAATCAGACAAAATAGTTTA+TGG | 0.211784 | 2.4:+5173112 | MS.gene85284:CDS |
| ATTCAGCATGAAAGTCTTTC+TGG | 0.219781 | 2.4:+5171678 | MS.gene85284:CDS |
| GATACATGGGCTTCTTCTTT+AGG | 0.225126 | 2.4:-5170136 | None:intergenic |
| ATCAGGACCTCCATTGTTTA+TGG | 0.225767 | 2.4:+5173942 | MS.gene85284:CDS |
| GCTTTCTTCAATCTGGGTTC+AGG | 0.240475 | 2.4:+5172052 | MS.gene85284:CDS |
| AAGAAAATGCTAACCTTAAC+AGG | 0.270752 | 2.4:-5174563 | None:intergenic |
| CTTGTATAATATAGCCAAAT+TGG | 0.273376 | 2.4:+5170335 | MS.gene85284:CDS |
| TGTGCGAAGCCAAGGTTAAA+AGG | 0.278558 | 2.4:-5172820 | None:intergenic |
| TTCCCTCAGGAGCATATCTT+TGG | 0.279931 | 2.4:+5172538 | MS.gene85284:CDS |
| TCTATTATTTACTCGTTCTA+AGG | 0.280983 | 2.4:+5172136 | MS.gene85284:CDS |
| TGAGCCAGTTGACATAGTTT+TGG | 0.281185 | 2.4:-5169222 | None:intergenic |
| ATGTATCCCAGTTACCTTCT+TGG | 0.288907 | 2.4:-5172562 | None:intergenic |
| CCTCGCACTGGTTCTTCATT+TGG | 0.289087 | 2.4:-5174430 | None:intergenic |
| CGGCTAAGAATCCTGCTTAT+TGG | 0.314950 | 2.4:+5170301 | MS.gene85284:CDS |
| TTGTTGTGATACAACACATT+AGG | 0.316010 | 2.4:-5171388 | None:intergenic |
| GCCGTCACTCTCTACCTTCT+TGG | 0.318765 | 2.4:-5171649 | None:intergenic |
| GCAGATGAAGTGCGGATCTT+AGG | 0.320739 | 2.4:+5168890 | MS.gene85284:CDS |
| CAACTTCTTGTAACGTTTAA+CGG | 0.322561 | 2.4:-5166065 | None:intergenic |
| TGAAATCTTACCCCGTAATA+AGG | 0.322697 | 2.4:+5166093 | MS.gene85284:CDS |
| CCATCTTATGTTGTCCATAT+TGG | 0.323999 | 2.4:+5170592 | MS.gene85284:CDS |
| GCCATTGCCTCATAATTTGC+AGG | 0.331368 | 2.4:-5172095 | None:intergenic |
| GAATACCTGATTCAGCTAAA+AGG | 0.333943 | 2.4:-5170476 | None:intergenic |
| CTTTCTTCAATCTGGGTTCA+GGG | 0.338815 | 2.4:+5172053 | MS.gene85284:CDS |
| ATCCAAGTGCTTGACCAATA+TGG | 0.341542 | 2.4:-5170606 | None:intergenic |
| CAGGGAACATCTGCTGAAAA+TGG | 0.345392 | 2.4:+5172071 | MS.gene85284:CDS |
| TTCAGGATCTAAGATCTCTT+AGG | 0.350091 | 2.4:+5172003 | MS.gene85284:intron |
| TGGTTCTTCATTTGGTCATA+AGG | 0.353179 | 2.4:-5174422 | None:intergenic |
| CCTGACCGTGAAACACAAAT+TGG | 0.353295 | 2.4:+5171555 | MS.gene85284:CDS |
| TACATACATGTTCAACTTAG+AGG | 0.356074 | 2.4:+5169191 | MS.gene85284:CDS |
| ACTACAAACTCTATCTTCTA+TGG | 0.359318 | 2.4:+5169290 | MS.gene85284:CDS |
| TTCTACCAGTTTCCCATTCA+AGG | 0.361845 | 2.4:-5171709 | None:intergenic |
| CAGTGCGAGGCCCTTGAAAC+AGG | 0.367467 | 2.4:+5174443 | MS.gene85284:CDS |
| CCAATTTGTGTTTCACGGTC+AGG | 0.367633 | 2.4:-5171555 | None:intergenic |
| AACCAACCGGTGCTTCTGTC+AGG | 0.368938 | 2.4:-5172904 | None:intergenic |
| TCTTACTAAAGCCATGTAAC+TGG | 0.377212 | 2.4:-5172498 | None:intergenic |
| ACCAACCGCCCAGATGTTCT+AGG | 0.381925 | 2.4:+5174182 | MS.gene85284:CDS |
| TCTCTTGCTTTCTTCAATCT+GGG | 0.383800 | 2.4:+5172046 | MS.gene85284:CDS |
| TGACTGGCTTCCACTTTGTT+CGG | 0.384685 | 2.4:-5174138 | None:intergenic |
| AGTTTCCCGTCAACCTGTTA+AGG | 0.390731 | 2.4:+5174550 | MS.gene85284:CDS |
| ATCAGAGAAATCGGCTGCTC+TGG | 0.390835 | 2.4:-5172858 | None:intergenic |
| CATGAAATTATACCTTATTA+CGG | 0.393853 | 2.4:-5166105 | None:intergenic |
| ATGAAATTATACCTTATTAC+GGG | 0.396896 | 2.4:-5166104 | None:intergenic |
| AATTGATGAAACAACGCATC+AGG | 0.397806 | 2.4:-5171513 | None:intergenic |
| TGATTAACACCTAGAACATC+TGG | 0.398163 | 2.4:-5174191 | None:intergenic |
| AACTTCTTGTAACGTTTAAC+GGG | 0.399434 | 2.4:-5166064 | None:intergenic |
| GTCACTATCAGCTGTACAAT+TGG | 0.399637 | 2.4:+5173165 | MS.gene85284:CDS |
| AGCTTTCAAGAATTTATCAT+AGG | 0.401656 | 2.4:-5174914 | None:intergenic |
| TGTTGTGATACAACACATTA+GGG | 0.403427 | 2.4:-5171387 | None:intergenic |
| TTTCTCTACCTTGGCAAGGT+TGG | 0.405784 | 2.4:-5170074 | None:intergenic |
| AGATCCCTTGAATGGGAAAC+TGG | 0.413750 | 2.4:+5171704 | MS.gene85284:CDS |
| TTGTAATATGCACATTTCAA+TGG | 0.414364 | 2.4:-5170840 | None:intergenic |
| GTGCTCCAATTTGTGTTTCA+CGG | 0.415252 | 2.4:-5171560 | None:intergenic |
| GAACCACTGGTCTTCTGAAA+AGG | 0.418783 | 2.4:+5170419 | MS.gene85284:CDS |
| TGCCATCCGTGGTTTCTCCT+TGG | 0.421597 | 2.4:+5171607 | MS.gene85284:CDS |
| TCATCAATTGCTACTGGCCA+TGG | 0.423313 | 2.4:+5171527 | MS.gene85284:CDS |
| CAAGCCTCAGTTGCAGTCAT+TGG | 0.424672 | 2.4:+5170710 | MS.gene85284:CDS |
| CCTTCATCTATCAAATCATC+TGG | 0.433965 | 2.4:-5174107 | None:intergenic |
| TATCCCAAAACTATGTCAAC+TGG | 0.436399 | 2.4:+5169218 | MS.gene85284:CDS |
| TAGGAAATTCTTGAACCAAC+TGG | 0.437576 | 2.4:-5170117 | None:intergenic |
| CCAGATGATTTGATAGATGA+AGG | 0.440649 | 2.4:+5174107 | MS.gene85284:CDS |
| CTGTTTCAAGGGCCTCGCAC+TGG | 0.440870 | 2.4:-5174442 | None:intergenic |
| CCTGATGATGCTTATCCATC+AGG | 0.442006 | 2.4:+5173925 | MS.gene85284:CDS |
| TGGAATCAGATCAGAGAAAT+CGG | 0.443201 | 2.4:-5172867 | None:intergenic |
| TTGTATCAGGTTGGAGATGC+AGG | 0.443500 | 2.4:+5171253 | MS.gene85284:intron |
| AACCACTGGTCTTCTGAAAA+GGG | 0.445232 | 2.4:+5170420 | MS.gene85284:CDS |
| CTATTTGCCAGTAGTTTGCT+AGG | 0.446027 | 2.4:+5168836 | MS.gene85284:CDS |
| TCTGGAGCAGATCCCTTGAA+TGG | 0.446950 | 2.4:+5171696 | MS.gene85284:CDS |
| ACTTGAAAGAACTATGGCCT+TGG | 0.447402 | 2.4:-5174516 | None:intergenic |
| GATAAATTCTTGAAAGCTGC+AGG | 0.448096 | 2.4:+5174919 | MS.gene85284:CDS |
| TCCACAGACATCCAGTTACA+TGG | 0.449793 | 2.4:+5172487 | MS.gene85284:intron |
| TGGGAAACTGGTAGAAGAAA+AGG | 0.453205 | 2.4:+5171716 | MS.gene85284:CDS |
| TCTAGGTGTTAATCAACTCT+TGG | 0.455100 | 2.4:+5174199 | MS.gene85284:CDS |
| TTCATTTGGTCATAAGGTAA+AGG | 0.457246 | 2.4:-5174416 | None:intergenic |
| GTAGTTTGCTAGGGATCATT+CGG | 0.460329 | 2.4:+5168846 | MS.gene85284:CDS |
| TGAATTCAGTTTCTCTACCT+TGG | 0.460514 | 2.4:-5170083 | None:intergenic |
| GAGCTCAATGATAGGAAAAT+CGG | 0.463220 | 2.4:+5167568 | MS.gene85284:CDS |
| AGAAAGACTTTCATGCTGAA+TGG | 0.464680 | 2.4:-5171676 | None:intergenic |
| CCTTAAATGTTTAATGAGAT+CGG | 0.465046 | 2.4:-5170741 | None:intergenic |
| GTCCATATTGGTCAAGCACT+TGG | 0.465263 | 2.4:+5170604 | MS.gene85284:CDS |
| TTCAAAAGGAGATTTGAAGT+TGG | 0.466183 | 2.4:+5174798 | MS.gene85284:CDS |
| TTTGGCCAGGTGAAGTTCAT+GGG | 0.467465 | 2.4:+5169694 | MS.gene85284:intron |
| TCATCCACCAATTCAAGAAA+AGG | 0.470757 | 2.4:-5173064 | None:intergenic |
| TTTAGTAAGATGCTTCCAAT+TGG | 0.474294 | 2.4:+5172511 | MS.gene85284:CDS |
| AACGACTGTGCGGCGTGTCT+TGG | 0.477917 | 2.4:+5170368 | MS.gene85284:CDS |
| CATTCAACATCGAAGATACA+TGG | 0.478977 | 2.4:-5170150 | None:intergenic |
| GTTGTTTCATCAATTGCTAC+TGG | 0.481617 | 2.4:+5171521 | MS.gene85284:CDS |
| CATATTACAACTTTCAATCA+AGG | 0.482382 | 2.4:+5170853 | MS.gene85284:CDS |
| CTATGGCCTTGGTCTCCTGC+TGG | 0.483473 | 2.4:-5174505 | None:intergenic |
| TGTTAATCAACTCTTGGAAT+CGG | 0.484775 | 2.4:+5174205 | MS.gene85284:CDS |
| CCAATATGGACAACATAAGA+TGG | 0.486702 | 2.4:-5170592 | None:intergenic |
| AGGTAAAGGAGTTGAGGAAG+TGG | 0.490305 | 2.4:-5174402 | None:intergenic |
| CTGGAGCAGATCCCTTGAAT+GGG | 0.491735 | 2.4:+5171697 | MS.gene85284:CDS |
| AATCTGAAGGATAGGTTGCT+TGG | 0.496345 | 2.4:-5170642 | None:intergenic |
| GAATCCTGCTTATTGGTCAA+AGG | 0.497986 | 2.4:+5170308 | MS.gene85284:CDS |
| GTCATAAGGTAAAGGAGTTG+AGG | 0.498611 | 2.4:-5174408 | None:intergenic |
| TTATACAAACAGCCCGCAGC+AGG | 0.499960 | 2.4:-5173090 | None:intergenic |
| CCTGATGGATAAGCATCATC+AGG | 0.500725 | 2.4:-5173925 | None:intergenic |
| GCTTTCAAGAATTTATCATA+GGG | 0.501037 | 2.4:-5174913 | None:intergenic |
| TTGTCAGATTACAGAGAACT+TGG | 0.504334 | 2.4:+5168182 | MS.gene85284:intron |
| TCAGCTGAAGCAACCGACAT+TGG | 0.505319 | 2.4:+5170779 | MS.gene85284:CDS |
| ATCTGAGCAAGTGGAGAACC+TGG | 0.507631 | 2.4:-5173976 | None:intergenic |
| AAATGGCCCTGCAAATTATG+AGG | 0.509961 | 2.4:+5172088 | MS.gene85284:CDS |
| TCGATGTTGAATGTTGCGAC+AGG | 0.513988 | 2.4:+5170160 | MS.gene85284:CDS |
| GTGCGAAGCCAAGGTTAAAA+GGG | 0.514025 | 2.4:-5172819 | None:intergenic |
| TAGTGTCTTGTGAACGGAAG+CGG | 0.514599 | 2.4:-5174855 | None:intergenic |
| AGGTTGCTGAGCTCAATGAT+AGG | 0.515256 | 2.4:+5167560 | MS.gene85284:intron |
| AAAATTGCTGTCGTCATGTA+GGG | 0.517807 | 2.4:+5168275 | MS.gene85284:CDS |
| ACATACATGTTCAACTTAGA+GGG | 0.518274 | 2.4:+5169192 | MS.gene85284:CDS |
| GAAAATTGCTGTCGTCATGT+AGG | 0.518574 | 2.4:+5168274 | MS.gene85284:CDS |
| GCAAGTGGAGAACCTGGCCT+TGG | 0.519764 | 2.4:-5173970 | None:intergenic |
| TCTACCAGTTTCCCATTCAA+GGG | 0.520413 | 2.4:-5171708 | None:intergenic |
| TCCTGAGGGAAAATGCCAAT+TGG | 0.521190 | 2.4:-5172526 | None:intergenic |
| TGCTAACCTTAACAGGTTGA+CGG | 0.526104 | 2.4:-5174556 | None:intergenic |
| TTGTCTTGATCTTGCACGCA+AGG | 0.526405 | 2.4:-5166041 | None:intergenic |
| ATAATATCAATCTGAAGGAT+AGG | 0.527266 | 2.4:-5170650 | None:intergenic |
| CAAACCTTTGACCAATAAGC+AGG | 0.528978 | 2.4:-5170312 | None:intergenic |
| AATGATCCCTAGCAAACTAC+TGG | 0.529789 | 2.4:-5168843 | None:intergenic |
| CTTGATCTTGCACGCAAGGA+AGG | 0.532038 | 2.4:-5166037 | None:intergenic |
| CCCTGCAAATTATGAGGCAA+TGG | 0.533075 | 2.4:+5172094 | MS.gene85284:CDS |
| CAGGCACTAGAATATTCAAA+AGG | 0.535759 | 2.4:+5174784 | MS.gene85284:intron |
| TCATCACTTTCACAGGGATA+CGG | 0.536957 | 2.4:+5170281 | MS.gene85284:intron |
| ACCTAGAACATCTGGGCGGT+TGG | 0.539791 | 2.4:-5174183 | None:intergenic |
| GCAGGCGGGAACAACCCGCC+TGG | 0.541194 | 2.4:-5165993 | None:intergenic |
| GCTCTGCTTCTACGTTCAGC+TGG | 0.545387 | 2.4:+5169267 | MS.gene85284:CDS |
| GATTTGATAGATGAAGGGAG+TGG | 0.545902 | 2.4:+5174113 | MS.gene85284:CDS |
| GATCTGCGTAATGAAAGCTT+TGG | 0.548201 | 2.4:+5168222 | MS.gene85284:CDS |
| ATAAACAATGGAGGTCCTGA+TGG | 0.548590 | 2.4:-5173940 | None:intergenic |
| TATAAGCATGTGCGAAGCCA+AGG | 0.550236 | 2.4:-5172828 | None:intergenic |
| CTTGGAGCAAAGATGTTACA+AGG | 0.550383 | 2.4:+5168200 | MS.gene85284:CDS |
| TTTATGGAGACACCAAGGCC+AGG | 0.551245 | 2.4:+5173958 | MS.gene85284:CDS |
| GCTAACCTTAACAGGTTGAC+GGG | 0.552218 | 2.4:-5174555 | None:intergenic |
| ACTCACTTTGTCAAAATCCA+TGG | 0.552219 | 2.4:-5169734 | None:intergenic |
| TCAGTTTCTCTACCTTGGCA+AGG | 0.555085 | 2.4:-5170078 | None:intergenic |
| AGCAAACTACTGGCAAATAG+TGG | 0.556008 | 2.4:-5168833 | None:intergenic |
| TGCACCAATGACTGCAACTG+AGG | 0.559030 | 2.4:-5170714 | None:intergenic |
| CTGGTTCTTAAAACTGCGAA+TGG | 0.560241 | 2.4:-5174486 | None:intergenic |
| AGCATCCCTGACAGAAGCAC+CGG | 0.563834 | 2.4:+5172898 | MS.gene85284:CDS |
| ATGTCAACTGGCTCAAGAAG+TGG | 0.563947 | 2.4:+5169230 | MS.gene85284:CDS |
| TATTTGCCAGTAGTTTGCTA+GGG | 0.566348 | 2.4:+5168837 | MS.gene85284:CDS |
| AATACAGAGGTTACCGCAGG+CGG | 0.566552 | 2.4:-5166008 | None:intergenic |
| ATTTGATAGATGAAGGGAGT+GGG | 0.568185 | 2.4:+5174114 | MS.gene85284:CDS |
| CCATTGCCTCATAATTTGCA+GGG | 0.571980 | 2.4:-5172094 | None:intergenic |
| GGTGGATGATAACCTGCTGC+GGG | 0.572873 | 2.4:+5173078 | MS.gene85284:CDS |
| GACTTTCATGCTGAATGGAA+AGG | 0.573928 | 2.4:-5171671 | None:intergenic |
| CAAAATAGTTTATGGATCTG+TGG | 0.575711 | 2.4:+5173120 | MS.gene85284:CDS |
| GAGGCTTACCACCATCTCGC+AGG | 0.575916 | 2.4:+5172792 | MS.gene85284:CDS |
| ACCAACCGGTGCTTCTGTCA+GGG | 0.578305 | 2.4:-5172903 | None:intergenic |
| GCTTCCTAGTGTCTTGTGAA+CGG | 0.579072 | 2.4:-5174861 | None:intergenic |
| TGTTAATAATATCAATCTGA+AGG | 0.579561 | 2.4:-5170655 | None:intergenic |
| AGGGACCTCCTGCGAGATGG+TGG | 0.582709 | 2.4:-5172800 | None:intergenic |
| TTATCATAGGGGCTCGAAGG+TGG | 0.586508 | 2.4:-5174901 | None:intergenic |
| CAGATGATTTGATAGATGAA+GGG | 0.588326 | 2.4:+5174108 | MS.gene85284:CDS |
| AAAAGGGACCTCCTGCGAGA+TGG | 0.588416 | 2.4:-5172803 | None:intergenic |
| AATTTATCATAGGGGCTCGA+AGG | 0.589435 | 2.4:-5174904 | None:intergenic |
| AGCATATCTTTGGACCAAGA+AGG | 0.591122 | 2.4:+5172548 | MS.gene85284:CDS |
| AGGCGGGTTGTTCCCGCCTG+CGG | 0.592808 | 2.4:+5165995 | MS.gene85284:CDS |
| TGTCAACTGGCTCAAGAAGT+GGG | 0.598481 | 2.4:+5169231 | MS.gene85284:CDS |
| TTGAACTAAAAGTAGACAGA+TGG | 0.600239 | 2.4:+5169168 | MS.gene85284:intron |
| ACCACTGGTCTTCTGAAAAG+GGG | 0.600634 | 2.4:+5170421 | MS.gene85284:CDS |
| TAATATAGCCAAATTGGCAA+AGG | 0.601822 | 2.4:+5170341 | MS.gene85284:CDS |
| GGGAACTCTATCTGAGCAAG+TGG | 0.603721 | 2.4:-5173985 | None:intergenic |
| CAATATGGACAACATAAGAT+GGG | 0.604028 | 2.4:-5170591 | None:intergenic |
| ATCTAAATCCAACCTTGCCA+AGG | 0.605271 | 2.4:+5170066 | MS.gene85284:CDS |
| ATACAGAGGTTACCGCAGGC+GGG | 0.606778 | 2.4:-5166007 | None:intergenic |
| TGGTGGATGATAACCTGCTG+CGG | 0.607006 | 2.4:+5173077 | MS.gene85284:CDS |
| TTCACAATCTCAGAACCAGT+TGG | 0.609615 | 2.4:+5170102 | MS.gene85284:CDS |
| TGAGCACTCACATCTATCCA+TGG | 0.610011 | 2.4:+5169717 | MS.gene85284:CDS |
| ATTGATGAAACAACGCATCA+GGG | 0.611434 | 2.4:-5171512 | None:intergenic |
| ATACGCATCATTTCCAATGT+CGG | 0.614408 | 2.4:-5170792 | None:intergenic |
| TAAGAACCAGCAGGAGACCA+AGG | 0.614641 | 2.4:+5174499 | MS.gene85284:CDS |
| TATGGTGCTCATGCCATCCG+TGG | 0.616911 | 2.4:+5171596 | MS.gene85284:CDS |
| GTCCAAAGATATGCTCCTGA+GGG | 0.617155 | 2.4:-5172540 | None:intergenic |
| CTTTCAAGAATTTATCATAG+GGG | 0.618755 | 2.4:-5174912 | None:intergenic |
| AGAGTTCCCTGATGTTGATG+AGG | 0.623066 | 2.4:+5173999 | MS.gene85284:CDS |
| GGTCCAAAGATATGCTCCTG+AGG | 0.625750 | 2.4:-5172541 | None:intergenic |
| GAATCCACTGAGAATTCCCA+AGG | 0.626646 | 2.4:+5167615 | MS.gene85284:CDS |
| TCCAAGAAGGTAGAGAGTGA+CGG | 0.634799 | 2.4:+5171648 | MS.gene85284:CDS |
| TATTATTAACATCACCACAC+AGG | 0.636418 | 2.4:+5170667 | MS.gene85284:CDS |
| CCACTGGTCTTCTGAAAAGG+GGG | 0.638125 | 2.4:+5170422 | MS.gene85284:CDS |
| AAGAAAGCAAGAGACTCACT+TGG | 0.642476 | 2.4:-5172037 | None:intergenic |
| TTCACGGTCAGGATGAGCCA+TGG | 0.645077 | 2.4:-5171544 | None:intergenic |
| GCTTCCGTTCACAAGACACT+AGG | 0.647855 | 2.4:+5174857 | MS.gene85284:CDS |
| CTTGGTGTCTCCATAAACAA+TGG | 0.651412 | 2.4:-5173952 | None:intergenic |
| ATTCAACATCGAAGATACAT+GGG | 0.652123 | 2.4:-5170149 | None:intergenic |
| ATTACCTTGGGAATTCTCAG+TGG | 0.654249 | 2.4:-5167619 | None:intergenic |
| GATTAACACCTAGAACATCT+GGG | 0.656514 | 2.4:-5174190 | None:intergenic |
| GTGATGTTCTACGATCTGAC+TGG | 0.658238 | 2.4:-5174154 | None:intergenic |
| GCTTACCACCATCTCGCAGG+AGG | 0.664540 | 2.4:+5172795 | MS.gene85284:CDS |
| GGGAGTGGGACCGAACAAAG+TGG | 0.665867 | 2.4:+5174128 | MS.gene85284:CDS |
| CAAAGGAAGCAACGACTGTG+CGG | 0.665878 | 2.4:+5170358 | MS.gene85284:CDS |
| AACCAAGGAGAAACCACGGA+TGG | 0.669181 | 2.4:-5171609 | None:intergenic |
| TAATGTGTTGTATCACAACA+AGG | 0.672528 | 2.4:+5171390 | MS.gene85284:CDS |
| GTCTAACCAAGGAGAAACCA+CGG | 0.677544 | 2.4:-5171613 | None:intergenic |
| AAACACGAGCAGATGAAGTG+CGG | 0.678176 | 2.4:+5168882 | MS.gene85284:CDS |
| GGTGTCTCCATAAACAATGG+AGG | 0.682652 | 2.4:-5173949 | None:intergenic |
| CATTGTTTATGGAGACACCA+AGG | 0.696387 | 2.4:+5173953 | MS.gene85284:CDS |
| CCAAATGAAGAACCAGTGCG+AGG | 0.703756 | 2.4:+5174430 | MS.gene85284:CDS |
| TGAAATTATACCTTATTACG+GGG | 0.709279 | 2.4:-5166103 | None:intergenic |
| TAACACCTAGAACATCTGGG+CGG | 0.824221 | 2.4:-5174187 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 5165980 | 5174948 | 5165980 | ID=MS.gene85284 |
| chr2.4 | mRNA | 5165980 | 5174948 | 5165980 | ID=MS.gene85284.t1;Parent=MS.gene85284 |
| chr2.4 | exon | 5165980 | 5166114 | 5165980 | ID=MS.gene85284.t1.exon1;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5165980 | 5166114 | 5165980 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5167562 | 5167636 | 5167562 | ID=MS.gene85284.t1.exon2;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5167562 | 5167636 | 5167562 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5168189 | 5168304 | 5168189 | ID=MS.gene85284.t1.exon3;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5168189 | 5168304 | 5168189 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5168832 | 5168946 | 5168832 | ID=MS.gene85284.t1.exon4;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5168832 | 5168946 | 5168832 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5169183 | 5169311 | 5169183 | ID=MS.gene85284.t1.exon5;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5169183 | 5169311 | 5169183 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5169703 | 5169750 | 5169703 | ID=MS.gene85284.t1.exon6;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5169703 | 5169750 | 5169703 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5170028 | 5170203 | 5170028 | ID=MS.gene85284.t1.exon7;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5170028 | 5170203 | 5170028 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5170296 | 5170492 | 5170296 | ID=MS.gene85284.t1.exon8;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5170296 | 5170492 | 5170296 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5170582 | 5170874 | 5170582 | ID=MS.gene85284.t1.exon9;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5170582 | 5170874 | 5170582 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5171262 | 5171411 | 5171262 | ID=MS.gene85284.t1.exon10;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5171262 | 5171411 | 5171262 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5171507 | 5171737 | 5171507 | ID=MS.gene85284.t1.exon11;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5171507 | 5171737 | 5171507 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5172008 | 5172157 | 5172008 | ID=MS.gene85284.t1.exon12;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5172008 | 5172157 | 5172008 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5172494 | 5172569 | 5172494 | ID=MS.gene85284.t1.exon13;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5172494 | 5172569 | 5172494 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5172792 | 5172919 | 5172792 | ID=MS.gene85284.t1.exon14;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5172792 | 5172919 | 5172792 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5173058 | 5173252 | 5173058 | ID=MS.gene85284.t1.exon15;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5173058 | 5173252 | 5173058 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5173880 | 5174020 | 5173880 | ID=MS.gene85284.t1.exon16;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5173880 | 5174020 | 5173880 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5174098 | 5174226 | 5174098 | ID=MS.gene85284.t1.exon17;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5174098 | 5174226 | 5174098 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5174368 | 5174571 | 5174368 | ID=MS.gene85284.t1.exon18;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5174368 | 5174571 | 5174368 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| chr2.4 | exon | 5174787 | 5174948 | 5174787 | ID=MS.gene85284.t1.exon19;Parent=MS.gene85284.t1 |
| chr2.4 | CDS | 5174787 | 5174948 | 5174787 | ID=cds.MS.gene85284.t1;Parent=MS.gene85284.t1 |
| Gene Sequence |
| Protein sequence |
