AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54899

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54899.t1 MTR_4g039320 97.763 1028 22 1 1 1027 1 1028 0.0 2076
MS.gene54899.t1 MTR_7g091780 51.420 1056 410 18 3 1027 7 990 0.0 969
MS.gene54899.t1 MTR_4g067110 44.719 1051 479 21 1 1027 1 973 0.0 828
MS.gene54899.t1 MTR_2g098850 43.667 1058 476 17 1 1027 1 969 0.0 812
MS.gene54899.t1 MTR_2g098850 55.019 518 214 2 1 499 1 518 0.0 617
MS.gene54899.t1 MTR_2g098850 38.702 416 228 10 619 1027 554 949 7.97e-63 231
MS.gene54899.t1 MTR_1g084090 34.167 1080 575 27 1 1027 1 997 0.0 553
MS.gene54899.t1 MTR_1g082810 50.000 98 49 0 40 137 2 99 1.73e-27 107
MS.gene54899.t1 MTR_1g082815 46.875 64 34 0 144 207 1 64 7.51e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54899.t1 AT5G21080 56.280 1059 387 19 1 1024 1 1018 0.0 1097
MS.gene54899.t1 AT2G41830 50.704 1065 441 12 2 1027 6 1025 0.0 1009
MS.gene54899.t1 AT2G41830 48.663 935 396 12 132 1027 1 890 0.0 813
MS.gene54899.t1 AT1G05960 50.984 508 230 4 1 493 1 504 1.67e-177 544
MS.gene54899.t1 AT1G05960 36.842 418 231 12 620 1027 588 982 7.71e-57 213
MS.gene54899.t1 AT1G05960 50.984 508 230 4 1 493 1 504 1.67e-177 544
MS.gene54899.t1 AT1G05960 36.842 418 231 12 620 1027 588 982 7.71e-57 213
MS.gene54899.t1 AT1G05960 50.984 508 230 4 1 493 1 504 1.67e-177 544
MS.gene54899.t1 AT1G05960 36.842 418 231 12 620 1027 588 982 7.71e-57 213
MS.gene54899.t1 AT5G26850 33.240 1068 583 27 1 1026 1 980 1.01e-174 536
MS.gene54899.t1 AT5G26850 33.240 1068 583 27 1 1026 1 980 1.01e-174 536
MS.gene54899.t1 AT5G26850 33.240 1068 583 27 1 1026 1 980 1.01e-174 536
MS.gene54899.t1 AT5G26850 33.240 1068 583 27 1 1026 1 980 1.01e-174 536
MS.gene54899.t1 AT1G05960 48.771 529 231 5 1 493 1 525 4.09e-172 530
MS.gene54899.t1 AT1G05960 36.842 418 231 12 620 1027 609 1003 7.09e-57 214

Find 208 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGCACTCTTTGAGAAAT+TGG 0.143744 4.4:-65844534 MS.gene54899:CDS
GCTGAGTGTTGATGACATTT+TGG 0.183306 4.4:-65842039 MS.gene54899:CDS
ACAATATCAAGTTGCATATT+AGG 0.203881 4.4:+65845355 None:intergenic
GGAAATCTTCTGTTGCTTTC+CGG 0.219003 4.4:+65841734 None:intergenic
ATCAAGGAAGATTCTCAAAA+TGG 0.237376 4.4:-65845885 MS.gene54899:CDS
TGGAGAGCCATTTCTTTATA+TGG 0.242690 4.4:+65841774 None:intergenic
AAAATATTTGTTACATTTGC+AGG 0.242854 4.4:+65842461 None:intergenic
AATTTCTTGTAACGTTTAAT+AGG 0.260530 4.4:+65848364 None:intergenic
TTCAATCTATTCATCATAGA+AGG 0.267853 4.4:+65844359 None:intergenic
ATGCCACGAACCCTGGATTT+TGG 0.270130 4.4:-65845710 MS.gene54899:CDS
TCAGGTTGTTTCGGTTGTTT+TGG 0.277392 4.4:-65845922 MS.gene54899:intron
CCTTGTACAGTTAACAATTA+AGG 0.283194 4.4:-65845152 MS.gene54899:intron
CATTACTTGCCAATTCATTC+AGG 0.289885 4.4:-65841692 MS.gene54899:CDS
GGATGATGAGGACGCTTTAA+AGG 0.298782 4.4:-65842561 MS.gene54899:CDS
TGTTTCACAGGTTTGGTTCA+TGG 0.302837 4.4:-65846512 MS.gene54899:intron
AAGAGTTTGCATACTAAATA+TGG 0.304713 4.4:-65845685 MS.gene54899:CDS
TTGCTGATCTTACGATCATT+AGG 0.317253 4.4:+65848173 None:intergenic
ATCAAGGTGCTTGACTAAAA+TGG 0.317663 4.4:+65845399 None:intergenic
GACGATGACATTCCAGCTTC+TGG 0.323469 4.4:-65842119 MS.gene54899:CDS
CTCCCATTTGTTGAGCTAGA+TGG 0.335829 4.4:+65846728 None:intergenic
ACAGAAGATTTCCACTTTCA+TGG 0.338277 4.4:-65841724 MS.gene54899:CDS
AGCATTTCTCTCAATGAAAA+TGG 0.338435 4.4:-65843466 MS.gene54899:intron
TCTTTATATGGCATGTTAGA+AGG 0.339140 4.4:+65841786 None:intergenic
AGCATCTTCTTCAACAATTA+CGG 0.339473 4.4:+65844292 None:intergenic
TCATCGTCAATAGTAAATAA+TGG 0.344171 4.4:+65842134 None:intergenic
AAGTTGTCATTTGTATTTAT+AGG 0.344443 4.4:-65847642 MS.gene54899:CDS
GGTTGATCCTTTCCTGCAAC+TGG 0.351301 4.4:-65842654 MS.gene54899:CDS
GGATGATAAACATTCTGATA+TGG 0.355282 4.4:-65842261 MS.gene54899:intron
GTACAGCATTTGCGAGCTTC+CGG 0.358129 4.4:-65846694 MS.gene54899:CDS
TCTTGAGCAAAGGTGTTATA+AGG 0.367302 4.4:-65847695 MS.gene54899:CDS
TGCTGTCATTAACCAGTTGC+AGG 0.367595 4.4:+65842642 None:intergenic
GCGTTATTTCGCGTAACATT+TGG 0.377850 4.4:-65848444 MS.gene54899:CDS
GTGCACCCACCCTGGTTTCA+TGG 0.382038 4.4:+65844687 None:intergenic
GATAGGGCAAGCCCGTGTTC+TGG 0.382269 4.4:+65845579 None:intergenic
TAAAACACTATCTTCTTGTA+TGG 0.383577 4.4:+65844496 None:intergenic
GCAGAGTGGAAATCCATTCT+TGG 0.384260 4.4:-65841504 MS.gene54899:CDS
TTAAGACATTCTTGTGATCA+AGG 0.387178 4.4:+65845383 None:intergenic
GTTTCACAGGTTTGGTTCAT+GGG 0.388563 4.4:-65846511 MS.gene54899:intron
ATTAACAACCAGCAGCCTTC+AGG 0.389547 4.4:+65841357 None:intergenic
TCCATTCTTGGATTCAAACT+TGG 0.390704 4.4:-65841492 MS.gene54899:CDS
TTGTTCTGATATGACAAATT+CGG 0.395585 4.4:+65844860 None:intergenic
CTGTTGTATCGAGAGATAAC+TGG 0.396788 4.4:+65842303 None:intergenic
TGACATTCCAGCTTCTGGTT+TGG 0.398635 4.4:-65842114 MS.gene54899:CDS
GGAATTCTACGCGTTATTCA+TGG 0.400204 4.4:-65845863 MS.gene54899:CDS
CCCAAATTGATGATAGCAAA+AGG 0.403707 4.4:+65844137 None:intergenic
CTTGTTAACTCAGGTTGTTT+CGG 0.407813 4.4:-65845931 MS.gene54899:intron
TATCAGTTGCCTTTGCCAAC+GGG 0.409820 4.4:+65844600 None:intergenic
GCATGTCGAGCAAGACAGAT+AGG 0.409849 4.4:+65845562 None:intergenic
AGGCAGCCAAGTAAAGCTTA+TGG 0.410878 4.4:-65842593 MS.gene54899:CDS
TCACAAAGTCAAACAAGGTT+TGG 0.411915 4.4:+65846946 None:intergenic
TCTCTTGATGATTCGAACTT+AGG 0.418749 4.4:-65845226 MS.gene54899:CDS
TGTTCAGCCAAGTTTGCCTT+TGG 0.422796 4.4:-65841619 MS.gene54899:intron
TGGCTGAACATTGGAATTTG+TGG 0.423136 4.4:+65841632 None:intergenic
TAACATGCCATATAAAGAAA+TGG 0.424091 4.4:-65841781 MS.gene54899:CDS
CCTTAATTGTTAACTGTACA+AGG 0.424842 4.4:+65845152 None:intergenic
AAGAGGAATCCTGAATGAAT+TGG 0.426773 4.4:+65841683 None:intergenic
CAAACTCTTGACCAAAATCC+AGG 0.427241 4.4:+65845699 None:intergenic
CAGCAATCTGTGGCTATAAT+CGG 0.434853 4.4:-65845295 MS.gene54899:CDS
AGGCACGTTGAGTTCTCCTT+TGG 0.438517 4.4:+65845824 None:intergenic
TTGCCATCTAGCTCAACAAA+TGG 0.438712 4.4:-65846731 MS.gene54899:CDS
AACTGATGAAATAGTGCCTC+GGG 0.438723 4.4:+65844734 None:intergenic
CAAAGCATTGGACCGGTCTC+AGG 0.441829 4.4:+65841446 None:intergenic
CAACATAAACTGCCGCAAAC+AGG 0.442152 4.4:+65848421 None:intergenic
AGCAGTACCAAAGGCAAACT+TGG 0.442672 4.4:+65841612 None:intergenic
CTTGCTAGTTGCTCGGAGTA+AGG 0.446580 4.4:-65844047 MS.gene54899:intron
AGAAGGGTTATTAACCTTTG+TGG 0.446634 4.4:+65844376 None:intergenic
TGGCTTTCCAAACCAGAAGC+TGG 0.447756 4.4:+65842107 None:intergenic
TCTATAAACTGCAGCAATCA+AGG 0.447846 4.4:+65844907 None:intergenic
CTCAAAGAGTGCTGCTGAAG+AGG 0.448504 4.4:+65844541 None:intergenic
TTTGATCCATAAGCTTTACT+TGG 0.449616 4.4:+65842587 None:intergenic
CCATTCTTGGATTCAAACTT+GGG 0.453203 4.4:-65841491 MS.gene54899:CDS
AATCTGTTGCTGATACAGTT+AGG 0.454028 4.4:-65842613 MS.gene54899:CDS
TGATGACTACTCAGCCTAAT+CGG 0.455645 4.4:+65844166 None:intergenic
CCCAAGTTTGAATCCAAGAA+TGG 0.456755 4.4:+65841491 None:intergenic
GTTTGACTTTGTGAACAATC+AGG 0.459893 4.4:-65846937 MS.gene54899:intron
CAGAAGATTTCCACTTTCAT+GGG 0.461514 4.4:-65841723 MS.gene54899:CDS
AGAGAGGATGTTGTAGGCCT+TGG 0.462324 4.4:+65842850 None:intergenic
CATGTCTAATATTACAGTTA+TGG 0.464441 4.4:-65844936 MS.gene54899:CDS
TTATCATATGGACGCGATGC+TGG 0.465130 4.4:+65841383 None:intergenic
GGAAAACTATGGGGATATCA+AGG 0.467639 4.4:-65845901 MS.gene54899:CDS
TGCCACAGCTTATCAGCAGC+AGG 0.469495 4.4:-65841423 MS.gene54899:CDS
AATGAGAGAGAGGATGTTGT+AGG 0.471509 4.4:+65842844 None:intergenic
CGTAACATTTGGCCTGTTTG+CGG 0.471842 4.4:-65848433 MS.gene54899:CDS
ACCGGATGATGCTTGTCCAC+TGG 0.473078 4.4:-65842330 MS.gene54899:CDS
TACAACTGTACGTCGTGTCC+TGG 0.473240 4.4:-65845643 MS.gene54899:CDS
CCGGATGATGCTTGTCCACT+GGG 0.473554 4.4:-65842329 MS.gene54899:CDS
CTTGATCTTTCCCGCAATGC+AGG 0.474405 4.4:+65848391 None:intergenic
AGGGAAGATGCCACGAACCC+TGG 0.476421 4.4:-65845717 MS.gene54899:CDS
CAGTTATCTCTCGATACAAC+AGG 0.477374 4.4:-65842302 MS.gene54899:CDS
TCAAGGAAGATTCTCAAAAT+GGG 0.477988 4.4:-65845884 MS.gene54899:CDS
GCTCAACAAATGGGAGATGA+TGG 0.478655 4.4:-65846721 MS.gene54899:CDS
TGTCACAGGTATTACCGATT+AGG 0.479604 4.4:-65844180 MS.gene54899:intron
AGCAAGCTGCTTGCAAACAA+AGG 0.480586 4.4:+65847029 None:intergenic
GATAATTTCCTGAAGGCTGC+TGG 0.482284 4.4:-65841365 MS.gene54899:CDS
ATGAATTGGCAAGTAATGAT+TGG 0.490570 4.4:+65841697 None:intergenic
AATAGCGGAACAAGGATTCC+AGG 0.490919 4.4:+65845625 None:intergenic
TATTATTCTACCGGCGTGCG+AGG 0.494328 4.4:+65848325 None:intergenic
GCCTTGTCTTGCTAGTTGCT+CGG 0.494617 4.4:-65844054 MS.gene54899:CDS
GCCTTCAGGAAATTATCATA+TGG 0.495284 4.4:+65841371 None:intergenic
ACGTACAGTTGTACCTTCCT+TGG 0.497866 4.4:+65845653 None:intergenic
TCAGGCAATGTGGTGGGTGA+CGG 0.498916 4.4:+65841464 None:intergenic
AAATCCTCTTCGTGTTCCTA+AGG 0.499125 4.4:-65848129 MS.gene54899:intron
GTTGTGCATCGGTACTAGCT+TGG 0.499618 4.4:+65842087 None:intergenic
ACGGCCATGGTATCGAGAAC+AGG 0.501724 4.4:+65844971 None:intergenic
CCCACCACATTGCCTGAGAC+CGG 0.501838 4.4:-65841458 MS.gene54899:CDS
TTGTATTTATAGGAAGTTGT+TGG 0.503668 4.4:-65847632 MS.gene54899:CDS
ACTTCAAGTCCTATCTTCAA+TGG 0.505588 4.4:-65846671 MS.gene54899:intron
TACGCGTTATTCATGGAGAA+TGG 0.506518 4.4:-65845856 MS.gene54899:CDS
GATTCAGTGCTCGTCAAAGC+AGG 0.507356 4.4:+65844398 None:intergenic
AGGATACCAGTCTGGACTAA+AGG 0.508989 4.4:-65842282 MS.gene54899:CDS
ATCATATTACCATTGAAGAT+AGG 0.509319 4.4:+65846662 None:intergenic
TTGGTTCCATTGTATTACTT+CGG 0.509496 4.4:+65845201 None:intergenic
CCCAGTGGACAAGCATCATC+CGG 0.509731 4.4:+65842329 None:intergenic
TCTTGTCCTTGTGCCATCGT+CGG 0.510962 4.4:-65844651 MS.gene54899:CDS
TAAAGGCTGTTGTTGTGCAT+CGG 0.510975 4.4:+65842076 None:intergenic
GGTCTGCATGAACCATTGCC+AGG 0.511315 4.4:+65844708 None:intergenic
CAATCTATAATTGAAAATGC+TGG 0.514670 4.4:-65845540 MS.gene54899:intron
CGATGCTGGCAGTTGAAAGA+AGG 0.515201 4.4:+65841397 None:intergenic
TATCATCCTTTAGTCCAGAC+TGG 0.515638 4.4:+65842276 None:intergenic
TCATCAGTTACTCCTGGCAA+TGG 0.516923 4.4:-65844720 MS.gene54899:CDS
TAGCAAACGATTCATTTGAC+TGG 0.518116 4.4:+65842510 None:intergenic
TCTTGGTTGTAATCAGGAAG+AGG 0.518222 4.4:+65841666 None:intergenic
TGACCAAAATCCAGGGTTCG+TGG 0.523303 4.4:+65845707 None:intergenic
GCCTCTTCACACTAGTTACT+CGG 0.527178 4.4:+65844324 None:intergenic
TCATTAACCAGTTGCAGGAA+AGG 0.527548 4.4:+65842647 None:intergenic
TCCTGATTACAACCAAGAAA+AGG 0.529816 4.4:-65841661 MS.gene54899:CDS
TGCCATCTAGCTCAACAAAT+GGG 0.530176 4.4:-65846730 MS.gene54899:CDS
GATACAACAGGATACCAGTC+TGG 0.531015 4.4:-65842290 MS.gene54899:CDS
AATATGCAACTTGATATTGT+CGG 0.532526 4.4:-65845352 MS.gene54899:CDS
TTCATGCAGACCATGAAACC+AGG 0.536903 4.4:-65844697 MS.gene54899:CDS
GGATTTGAAGAAGAAGGTTG+AGG 0.537297 4.4:+65844623 None:intergenic
TGAATTGGCAAGTAATGATT+GGG 0.541834 4.4:+65841698 None:intergenic
AGGCAAACTTGGCTGAACAT+TGG 0.542103 4.4:+65841623 None:intergenic
TTGCGATAGAAATGAGAGAG+AGG 0.542283 4.4:+65842834 None:intergenic
ACCGGTCTCAGGCAATGTGG+TGG 0.544210 4.4:+65841457 None:intergenic
ACTATTTCATCAGTTACTCC+TGG 0.544261 4.4:-65844726 MS.gene54899:CDS
ATTATTCTACCGGCGTGCGA+GGG 0.544297 4.4:+65848326 None:intergenic
ATATCAGTTGCCTTTGCCAA+CGG 0.544918 4.4:+65844599 None:intergenic
TAAGCTGTGGCACAAAGCAT+TGG 0.545776 4.4:+65841434 None:intergenic
AAATGTCATCAACACTCAGC+AGG 0.545797 4.4:+65842042 None:intergenic
TGAAGATAGGACTTGAAGTC+CGG 0.547854 4.4:+65846675 None:intergenic
CCTGCAGAATTCTTCTCATG+AGG 0.550469 4.4:-65843527 MS.gene54899:intron
CCAAATTGATGATAGCAAAA+GGG 0.553351 4.4:+65844138 None:intergenic
ATTGAGAGAAATGCTCCTCA+AGG 0.556592 4.4:+65843474 None:intergenic
TGATTGGGCACCCATGAAAG+TGG 0.559105 4.4:+65841713 None:intergenic
TCTTCTGTTGCTTTCCGGCG+AGG 0.560458 4.4:+65841739 None:intergenic
TAAAGCTTATGGATCAAAAG+AGG 0.560884 4.4:-65842582 MS.gene54899:CDS
ACTATGATAGTGCAGAGTCT+AGG 0.562791 4.4:-65842488 MS.gene54899:CDS
CATTGTGAGAACCTCCTCGC+CGG 0.567146 4.4:-65841753 MS.gene54899:CDS
CGGCGAGGAGGTTCTCACAA+TGG 0.570303 4.4:+65841754 None:intergenic
CCGGTCTCAGGCAATGTGGT+GGG 0.575594 4.4:+65841458 None:intergenic
GGCCTGCTGCTGATAAGCTG+TGG 0.575807 4.4:+65841421 None:intergenic
AGACAACACACCACGTAGGA+AGG 0.575883 4.4:-65841824 MS.gene54899:CDS
TGACTTCAATCTATTAAGAA+CGG 0.576842 4.4:+65844451 None:intergenic
AACTGCAAGCATCACGCCGA+AGG 0.577152 4.4:-65842913 MS.gene54899:CDS
TAACTGATGAAATAGTGCCT+CGG 0.577843 4.4:+65844733 None:intergenic
TCAATCTATTCATCATAGAA+GGG 0.579432 4.4:+65844360 None:intergenic
GTTATGCAGATCTCAGATGC+AGG 0.580061 4.4:-65844995 MS.gene54899:intron
GTGTTCTGGAGACCAGAGAT+TGG 0.580951 4.4:+65845593 None:intergenic
CATGTCGAGCAAGACAGATA+GGG 0.585911 4.4:+65845563 None:intergenic
TGTGGCACAAAGCATTGGAC+CGG 0.594211 4.4:+65841439 None:intergenic
ATGGCTAAGTTAGCCAAGGA+AGG 0.594419 4.4:-65845666 MS.gene54899:CDS
AAAGGTGTTATAAGGAGTTG+AGG 0.597065 4.4:-65847687 MS.gene54899:CDS
AATCTCTGGTCTCCAGAACA+CGG 0.597119 4.4:-65845591 MS.gene54899:CDS
AATCATCCGTGTTAAAAGAA+CGG 0.601503 4.4:-65842370 MS.gene54899:CDS
TGCTGGCAGTTGAAAGAAGG+CGG 0.602146 4.4:+65841400 None:intergenic
TGGATCAAAAGAGGATGATG+AGG 0.602970 4.4:-65842573 MS.gene54899:CDS
CATGATTGTCTTCACATCCA+AGG 0.606881 4.4:-65842867 MS.gene54899:CDS
TAACAAGCTATCTTGAGCAA+AGG 0.610121 4.4:-65847705 MS.gene54899:CDS
GCCGAGTAACTAGTGTGAAG+AGG 0.610135 4.4:-65844325 MS.gene54899:CDS
TATGCGATGTGCACCCACCC+TGG 0.610716 4.4:+65844679 None:intergenic
GACAAACCGACGATGGCACA+AGG 0.610906 4.4:+65844645 None:intergenic
AAATATGGCTAAGTTAGCCA+AGG 0.613203 4.4:-65845670 MS.gene54899:CDS
TCCATATGATAATTTCCTGA+AGG 0.615364 4.4:-65841372 MS.gene54899:CDS
GATTGAAGTCAAGTTATAGT+CGG 0.616954 4.4:-65844439 MS.gene54899:CDS
CTTGGATGTGAAGACAATCA+TGG 0.617532 4.4:+65842868 None:intergenic
ATCTCTGGTCTCCAGAACAC+GGG 0.621594 4.4:-65845590 MS.gene54899:CDS
TCTGTTGCTTTCCGGCGAGG+AGG 0.621680 4.4:+65841742 None:intergenic
CTAGTGTAAAGAGAGACCTT+CGG 0.623227 4.4:+65842897 None:intergenic
TCAGTTGCCTTTGCCAACGG+GGG 0.623574 4.4:+65844602 None:intergenic
ATCAGTTGCCTTTGCCAACG+GGG 0.625914 4.4:+65844601 None:intergenic
ATTGTTCACAAAGTCAAACA+AGG 0.626698 4.4:+65846941 None:intergenic
AAATTCTTCTTGATCAATCG+CGG 0.626834 4.4:-65846995 MS.gene54899:CDS
TCAACAATTACGGATACTTG+AGG 0.628038 4.4:+65844302 None:intergenic
ATTATCAAAATAGCGGAACA+AGG 0.628643 4.4:+65845617 None:intergenic
AACAAGAGTTCAGCAATCTG+TGG 0.636393 4.4:-65845305 MS.gene54899:CDS
AAACTCTTGACCAAAATCCA+GGG 0.643801 4.4:+65845700 None:intergenic
GATTGGTATTATCAAAATAG+CGG 0.647821 4.4:+65845610 None:intergenic
AGGTCCTGTTCTCGATACCA+TGG 0.653165 4.4:-65844975 MS.gene54899:CDS
TAGGAACCGAAGTAATACAA+TGG 0.653776 4.4:-65845207 MS.gene54899:CDS
AACTAGCAAGACAAGGCTGT+AGG 0.667594 4.4:+65844060 None:intergenic
TGGACCGGTCTCAGGCAATG+TGG 0.668100 4.4:+65841454 None:intergenic
GCAGACCATGAAACCAGGGT+GGG 0.677610 4.4:-65844692 MS.gene54899:CDS
TCCGAGCAACTAGCAAGACA+AGG 0.678440 4.4:+65844053 None:intergenic
AGATATCTTCCCTCGCACGC+CGG 0.678645 4.4:-65848335 MS.gene54899:intron
CATACCTTAGGAACACGAAG+AGG 0.681157 4.4:+65848125 None:intergenic
TGCAGACCATGAAACCAGGG+TGG 0.685737 4.4:-65844693 MS.gene54899:CDS
TCATGCAGACCATGAAACCA+GGG 0.686293 4.4:-65844696 MS.gene54899:CDS
AGAATGGTAGTGAATGCCAA+AGG 0.687586 4.4:-65845840 MS.gene54899:CDS
GGTTGAGGACAAACCGACGA+TGG 0.687924 4.4:+65844638 None:intergenic
AGAGATAACTGGACTCCCAG+TGG 0.689865 4.4:+65842314 None:intergenic
GAATTTGTCATATCAGAACA+AGG 0.697630 4.4:-65844858 MS.gene54899:intron
GACGAGCACTGAATCCACAA+AGG 0.700276 4.4:-65844390 MS.gene54899:CDS
TTGGAGACAACACACCACGT+AGG 0.734551 4.4:-65841828 MS.gene54899:CDS
TATTCTTTAGGCATTCCCCG+AGG 0.741207 4.4:-65844750 MS.gene54899:intron
ACTGATGAAATAGTGCCTCG+GGG 0.754949 4.4:+65844735 None:intergenic
AAACAGAAATCCTTCCTACG+TGG 0.759461 4.4:+65841814 None:intergenic
GTGATATGATGAGACACCTG+CGG 0.773872 4.4:-65845264 MS.gene54899:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.4 gene 65841358 65848470 65841358 ID=MS.gene54899
chr4.4 mRNA 65841358 65848470 65841358 ID=MS.gene54899.t1;Parent=MS.gene54899
chr4.4 exon 65848336 65848470 65848336 ID=MS.gene54899.t1.exon1;Parent=MS.gene54899.t1
chr4.4 CDS 65848336 65848470 65848336 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65848130 65848204 65848130 ID=MS.gene54899.t1.exon2;Parent=MS.gene54899.t1
chr4.4 CDS 65848130 65848204 65848130 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65847613 65847728 65847613 ID=MS.gene54899.t1.exon3;Parent=MS.gene54899.t1
chr4.4 CDS 65847613 65847728 65847613 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65846938 65847052 65846938 ID=MS.gene54899.t1.exon4;Parent=MS.gene54899.t1
chr4.4 CDS 65846938 65847052 65846938 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65846672 65846800 65846672 ID=MS.gene54899.t1.exon5;Parent=MS.gene54899.t1
chr4.4 CDS 65846672 65846800 65846672 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65846477 65846524 65846477 ID=MS.gene54899.t1.exon6;Parent=MS.gene54899.t1
chr4.4 CDS 65846477 65846524 65846477 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65845822 65845940 65845822 ID=MS.gene54899.t1.exon7;Parent=MS.gene54899.t1
chr4.4 CDS 65845822 65845940 65845822 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65845541 65845737 65845541 ID=MS.gene54899.t1.exon8;Parent=MS.gene54899.t1
chr4.4 CDS 65845541 65845737 65845541 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65845153 65845445 65845153 ID=MS.gene54899.t1.exon9;Parent=MS.gene54899.t1
chr4.4 CDS 65845153 65845445 65845153 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65844859 65845008 65844859 ID=MS.gene54899.t1.exon10;Parent=MS.gene54899.t1
chr4.4 CDS 65844859 65845008 65844859 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65844277 65844762 65844277 ID=MS.gene54899.t1.exon11;Parent=MS.gene54899.t1
chr4.4 CDS 65844277 65844762 65844277 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65844048 65844194 65844048 ID=MS.gene54899.t1.exon12;Parent=MS.gene54899.t1
chr4.4 CDS 65844048 65844194 65844048 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65843467 65843542 65843467 ID=MS.gene54899.t1.exon13;Parent=MS.gene54899.t1
chr4.4 CDS 65843467 65843542 65843467 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65842811 65842938 65842811 ID=MS.gene54899.t1.exon14;Parent=MS.gene54899.t1
chr4.4 CDS 65842811 65842938 65842811 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65842475 65842675 65842475 ID=MS.gene54899.t1.exon15;Parent=MS.gene54899.t1
chr4.4 CDS 65842475 65842675 65842475 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65842262 65842393 65842262 ID=MS.gene54899.t1.exon16;Parent=MS.gene54899.t1
chr4.4 CDS 65842262 65842393 65842262 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65842034 65842165 65842034 ID=MS.gene54899.t1.exon17;Parent=MS.gene54899.t1
chr4.4 CDS 65842034 65842165 65842034 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65841620 65841853 65841620 ID=MS.gene54899.t1.exon18;Parent=MS.gene54899.t1
chr4.4 CDS 65841620 65841853 65841620 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
chr4.4 exon 65841358 65841528 65841358 ID=MS.gene54899.t1.exon19;Parent=MS.gene54899.t1
chr4.4 CDS 65841358 65841528 65841358 ID=cds.MS.gene54899.t1;Parent=MS.gene54899.t1
Gene Sequence

>MS.gene54899

ATGAGCGTTATTTCGCGTAACATTTGGCCTGTTTGCGGCAGTTTATGTTGTTTTTGTCCTGCATTGCGGGAAAGATCAAGACATCCTATTAAACGTTACAAGAAATTGCTCGCAGATATCTTCCCTCGCACGCCGGTAGAATAATAATAACTTTTTTTTCTTTTTTTTCATTTATACGATAATTTGTAATTTTAATGTTATTTGTAGTTCTGAAATTTATAATCACAATGATAATAATGATGATGATGATCGTGTTGTTGTAACAGGAAGAAGAACCTAATGATCGTAAGATCAGCAAGTTATGCGAGTATGCATCGAAAAATCCTCTTCGTGTTCCTAAGGTATGTGATGAATTGTATTAATTGTAAAATGGAATTGGAATTGGAACTATTATTTCTTTTTTTCTTATGAGTAAGACCCAATTCAGTCCCTCATAATTTTCTTGTGCTGCAATTCAATCCTTGACAAAAAAAAATGCCCAAATTACTCACCAACATTTTCATACTTAGGACAAATTAGTCCCTCTAACATAATGATATAGGGACTAATTTATCCTAGCAGGGACTAATTTGAGTTTAATTTTTGTCGGGCACCGAATTGTGCCATTAGAAAATTGTGAGGAATCAAGTTGGGTTTTCACTCCTTGTTGAATAGCCCCGGGGGCAATAATTGTAGTAAGGAGGGATCATGAGGCCAAGAATCAAGAGTAGCACAATTTTTCACCCTTTGGTTGTTTTTACAGATAACAAGCTATCTTGAGCAAAGGTGTTATAAGGAGTTGAGGACTGAGAACTATCAAGCTGTGAAAGTTGTCATTTGTATTTATAGGAAGTTGTTGGTTTCTTGTAGAGATCAAATGTGAGTTCTTTTTTCATCATAGTTATCATTCTGTTGATATAATAAGTAGGGATCTTAGTTTGTAATTGCCAACTTAAAAATTAATAAAATTTGATGGTTAATTGATAGTCAGCATCATATGTTTTATGATTGTTAGTAAAGTATCTAATTAGTATCTTATATGACTTGATGTTCTAATCAAGTGATTAGGCTCAAGCGGTTAACGAGCTTCAGTTAAGGAGATCGTTCGGAAGAACTCGAGTTCTAATCCTGACTGGAACAGTTATTGGTCAAACTTTACTTACCTCCCAGCTAAACTCCGGATTATCAGGGCCCTCTTTCCTTGGGAACCTGAGGGTTAGGAAAAAAAAATTGATTTGATGTTTTTTTAATTTAATATATGAAGGAAATGTATTTATTTAGAAGTGTAAGAGCTTGGTTGTTTTATAGGCTCAAGTTTACTTTGGTTTGCAACCATTTGAAAAATAGTCACCTATGGAAATAACAGAAGCTGATTTTTCAACTTATTATGATTAAAAACATTTTATTGCGTATGATTTATGAGAACACTATTGTGGCAGGCCTTTGTTTGCAAGCAGCTTGCTTAGCATCATTCAAATTCTTCTTGATCAATCGCGGCAAGACGAAGTGCAGATTTTAGGATGCCAAACCTTGTTTGACTTTGTGAACAATCAGGTGGGGAATTTTTGGTAGGTTCTCAGTTTATGAGTTTGATGCTAGTTCACTCTTTCCTTAAAGTAGTTAAATGTTTACAACATTGTAAGTTTTTCCACATTGAAGTTTGATCTTGTATCATTCTTTTATTTGTGCAGAGGGATGGTACTTATATGTTTAATTTAGACAGTTTCATTTCAAAACTTTGCCATCTAGCTCAACAAATGGGAGATGATGGAAAAGTACAGCATTTGCGAGCTTCCGGACTTCAAGTCCTATCTTCAATGGTAATATGATTTCTACTTTATCATGTCTGCGATATGGCCATCAAGTCAAACAGCAACAAATCCAATCGATGCGCCAAAAGACCCCATTGAAAAAGGCTACTGTTATTGCTGATGTAAAATTGATAACATTCTTTTCTTGTTTCACAGGTTTGGTTCATGGGAGAATTCACTCATATTTCAGTTGAATTTGACAATGTAAGTTATTGATCTGTTTACATTTTCTTTCAAGGTATTAGTCATTTCGCACGGAGAAAAGAACACATTAGTGCTTGATTTTAGTTTTTTGGTACTTCTCAAATCAGCGAACTTTCTTTGACCTCAAACTAGGTGTTTAGTGGAGAAATTCATCTAGTTTTTTTTTCATGATTAACTTATTTTCGATAGAGGGGTAACCTTGGCGCAACTGGTAAAGTTGTTGTCATGTGACTAAAAGGTCACGGGTTCAAGTCCTGGAAACAACCTCTTGTGTAAAACAGGGTAAGGCTGCATACAATACACCAAATAGTGGGACCCCTTCCCGGACCCTGCATATGCGGGAGCTCTAGTGCACCGGGTTGCCCTTTTTAACTTATTTTCGATAACTTTCATTATATTACATTCTGATTGTGAGAGATAAACACCTCTATTGACTAAAATCGTTTCCGAACATCGTATAACACTTCCAATTACCTCTTGTCTTACCCTCCCGTATACTTCTTTCATTTGACAGCTTGCATCTCTTGTTAACTCAGGTTGTTTCGGTTGTTTTGGAAAACTATGGGGATATCAAGGAAGATTCTCAAAATGGGAATTCTACGCGTTATTCATGGAGAATGGTAGTGAATGCCAAAGGAGAACTCAACGTGCCTATGTAACTGTCCTTCTTGTTATATTAGCTTTTGTTTTGAGTTACTTGTATGTTATAATTGATGTTAACTTCATTTCTCTTCCATAGGGAAGATGCCACGAACCCTGGATTTTGGTCAAGAGTTTGCATACTAAATATGGCTAAGTTAGCCAAGGAAGGTACAACTGTACGTCGTGTCCTGGAATCCTTGTTCCGCTATTTTGATAATACCAATCTCTGGTCTCCAGAACACGGGCTTGCCCTATCTGTCTTGCTCGACATGCAATCTATAATTGAAAATGCTGGTATTTATTTTCTAGAGTGCTTTGAGATCAGATTCCCGTCAATGTTTCTTTATCCTATCTAAAGAGTTTGAAATTTGGTTTGCCGTAACTTACAGGACAGAATACACACTTGTTACTATCCATTTTAGTCAAGCACCTTGATCACAAGAATGTCTTAAAGAATCCTAATATGCAACTTGATATTGTCGGTGTTATTACCCATCTTGCTGAAAAAACAAGAGTTCAGCAATCTGTGGCTATAATCGGTGCATTGAGTGATATGATGAGACACCTGCGGAAAAGCATACACTGCTCTCTTGATGATTCGAACTTAGGAACCGAAGTAATACAATGGAACCAAAAATATAGAACGGAAGTAGATGAGTGCCTTGTACAGTTAACAATTAAGGTGACAAATTCCAACTTTTAAGATGAACTTTTTTGTTAATAATGTAACCGACCAGATAAGCGTGTAGTTCTGGATGCTGATTTGGCTGTGAATCTTAGATGTTCAATGTTAAGTCGTCTAATGTTTGGCTGTAATGTTATGCAGATCTCAGATGCAGGTCCTGTTCTCGATACCATGGCCGTGTTGTTAGAAAACATGTCTAATATTACAGTTATGGCAAGAACCTTGATTGCTGCAGTTTATAGAACTTCTCAAATAGTTGCATCTATACCGAATTTGTCATATCAGAACAAGGCAAGTTACTTAAATTGCCTGATTTGTAATAACCGTAATATTTCCTTACTTCTTTCTTTCTCATTTTTTCTGTAAACTTGAAACTCTATTCTTTAGGCATTCCCCGAGGCACTATTTCATCAGTTACTCCTGGCAATGGTTCATGCAGACCATGAAACCAGGGTGGGTGCACATCGCATATTTTCTCTTGTCCTTGTGCCATCGTCGGTTTGTCCTCAACCTTCTTCTTCAAATCCCCCGTTGGCAAAGGCAACTGATATTCAAAGAATGCTCTCGAGAAATGTCTCTGTATTTTCCTCTTCAGCAGCACTCTTTGAGAAATTGGAGAAGAAGCAACTTTCCATACAAGAAGATAGTGTTTTAGATGGAAAGAGCAATGATAGCTCCGTTCTTAATAGATTGAAGTCAAGTTATAGTCGGACTGCTAGTATAAGAAAACCTGCTTTGACGAGCACTGAATCCACAAAGGTTAATAACCCTTCTATGATGAATAGATTGAAGTCAAGTTACAGCCGAGTAACTAGTGTGAAGAGGCCTCAAGTATCCGTAATTGTTGAAGAAGATGCTACAACTAAAAAACAGGTATGTATTTGCACTATTTTACTTTACATTTTATTTTAATAACAATATAACTTATCAATCAAACACCATCACCATGTCACAGGTATTACCGATTAGGCTGAGTAGTCATCAGATTACCCTTTTGCTATCATCAATTTGGGTTCAGTCTATCTATCCTCTGAATACACCTGAAAATTTCGAAGCAATTGCTCATACCTACAGCCTTGTCTTGCTAGTTGCTCGGAGTAAGGTAATGTAAAAATTCTTGATATTTTACCATGTATACATTGAATGAGAAAATTGTTTTCTTGTATGGACTAGTTGACAGATTTTTAACACAGTCCTTTCAGTTTACCGACTTTCTGATAAAAGTTTGATAATTTAACTTTTAACAGAATAAAAATCGCCTCATTAACATATATATTTTCCTAATGCGGTTATTTAATTTTTTCGGTTATTTCAATTTATTTGAGAATGATTCTTATACCTGCGTCCTTGGTGATAGATAGTGTAGATGATTATTCATAAAGTTGTCTTAAGATTAAAATCAACCTCAGATAATTTACTCATACAATAACTTGCAAGTTATATCAATGGTCAAAATCAAAGTTATAAATTTTAATGTTACTTCAAGAATTTCCATTTCCATAAAAATGTGACACACCAACACACACACAAAAGCATTCATATCTCACATATATTTTTAAGTTTATCTTTATCTTATCCTCATAACTTGTATGCTTTGGCTCCTGCAGAATTCTTCTCATGAGGCTCTAATTCAAAGTTTTCAGTTGGCATTTTCCTTGAGGAGCATTTCTCTCAATGAAAATGGTAGAGTCCTCCGAATCCATATTATTTAATGTTTGTATGTTACCATGTTTTTTGTAAGAATGACAGCTCAATATCCGCGACATATGATATGGATATCTTATGAACCCATGTTTAGAATCACATGACTTTGTTCCTATTTTTTCCTTCCAAGGGAAGGTTTAGTGCTAAGGAAAGAAAACTGATAGAGTACGGAAATTAAGTGAAAGGAGGAATGTATTAAATTAAAAAGTAGACTAAGCTCTCACATACATGGAAAAAGTCTAATGCGGCGCATGATGTAATATACTTAACAGGGAAGTTTTGCAGTTAAGATTTTCCATGATATACAAAATTCATCAGATTATTTCTTACATACTCGACCAAATAGAACTGAGGTTATACAGAAAATATTTTATTTATCATGGCATATGTTCTCATATTAGATACAGTTTATTCATGTTTATTTTATGTTGCTTTTTGATGAAATGGCTTAAAATTGTGAATCATTGTTAAGGTGCCTAGTTTTGAAACACTTCTTTCTTTTTTCAGTTAAACTGCAAGCATCACGCCGAAGGTCTCTCTTTACACTAGCAACATCCATGATTGTCTTCACATCCAAGGCCTACAACATCCTCTCTCTCATTTCTATCGCAAAAATGGCATTGACAGATAAAACTGTATGTACATCTTGTTTCGTACAATGTTTTGCATTTAATTTGTCCAATTTTTCTTCCTTCTCTACTTTCTAGCGGTATTTTATATAATAAAATCACTCCTTTGTATCTGTTATAGTTTTTTATGCTTCTTTTCAGGTTGATCCTTTCCTGCAACTGGTTAATGACAGCAAGTTACAATCTGTTGCTGATACAGTTAGGCAGCCAAGTAAAGCTTATGGATCAAAAGAGGATGATGAGGACGCTTTAAAGGCACTTTCATCGATAAAAATAACTGAAAGCCAGTCAAATGAATCGTTTGCTACTATGATAGTGCAGAGTCTAGGAAAACCTGCAAATGTAACAAATATTTTTTTATCATTTAATGTTTCACTAGTTTATCAGGATCAATGTATTAACTTACATTTTTTTGTGCATCAGGAATCATCCGTGTTAAAAGAACGGCTTCTTAGTAATTTTACACCGGATGATGCTTGTCCACTGGGAGTCCAGTTATCTCTCGATACAACAGGATACCAGTCTGGACTAAAGGATGATAAACATTCTGATATGGTAATTTGCACAGCTCTCCTCATCCGCGTTTTTTCTTGGAAAAGAATATATTTCGTGTGTTTTAACCTGTACAGTCGCCTACATGAAATTATGCAGGTTGATATTCCATTATTTACTATTGACGATGACATTCCAGCTTCTGGTTTGGAAAGCCAAGCTAGTACCGATGCACAACAACAGCCTTTAGAAAATCTAAGCCTGCTGAGTGTTGATGACATTTTGGGTTCAGTAAGTTTTTCTGTTCATTGATAATTACACACCGATAACATATTTGATTTTAATTAAGCGATAGAGAAAAATGAAAAAACATTCTCTTAACGCTCATCCAATTATCTCTTCAATTTCCTCATCGAGTTATTCTAAAAATTCTGAAAAATGATGAGTCGTAAAACGATAATTCATATGCAGGTTTTGGAGACAACACACCACGTAGGAAGGATTTCTGTTTCAACACCTTCTAACATGCCATATAAAGAAATGGCTCTCCATTGTGAGAACCTCCTCGCCGGAAAGCAACAGAAGATTTCCACTTTCATGGGTGCCCAATCATTACTTGCCAATTCATTCAGGATTCCTCTTCCTGATTACAACCAAGAAAAGGACGAATCCACAAATTCCAATGTTCAGCCAAGTTTGCCTTTGGTACTGCTTTACTTTCTTTACTTTCGAAATTCAGAACAAGTGTTTCTTCTTGCTTTGAGATATCTTATTCCTGTTTCCTGTGCCATTTTAGTTGCAGAGTGGAAATCCATTCTTGGATTCAAACTTGGGCGCTCCGTCACCCACCACATTGCCTGAGACCGGTCCAATGCTTTGTGCCACAGCTTATCAGCAGCAGGCCGCCTTCTTTCAACTGCCAGCATCGCGTCCATATGATAATTTCCTGAAGGCTGCTGGTTGTTAA
Protein sequence

>MS.gene54899.t1

MSVISRNIWPVCGSLCCFCPALRERSRHPIKRYKKLLADIFPRTPEEEPNDRKISKLCEYASKNPLRVPKITSYLEQRCYKELRTENYQAVKVVICIYRKLLVSCRDQMPLFASSLLSIIQILLDQSRQDEVQILGCQTLFDFVNNQRDGTYMFNLDSFISKLCHLAQQMGDDGKVQHLRASGLQVLSSMVWFMGEFTHISVEFDNVVSVVLENYGDIKEDSQNGNSTRYSWRMVVNAKGELNVPMEDATNPGFWSRVCILNMAKLAKEGTTVRRVLESLFRYFDNTNLWSPEHGLALSVLLDMQSIIENAGQNTHLLLSILVKHLDHKNVLKNPNMQLDIVGVITHLAEKTRVQQSVAIIGALSDMMRHLRKSIHCSLDDSNLGTEVIQWNQKYRTEVDECLVQLTIKISDAGPVLDTMAVLLENMSNITVMARTLIAAVYRTSQIVASIPNLSYQNKAFPEALFHQLLLAMVHADHETRVGAHRIFSLVLVPSSVCPQPSSSNPPLAKATDIQRMLSRNVSVFSSSAALFEKLEKKQLSIQEDSVLDGKSNDSSVLNRLKSSYSRTASIRKPALTSTESTKVNNPSMMNRLKSSYSRVTSVKRPQVSVIVEEDATTKKQVLPIRLSSHQITLLLSSIWVQSIYPLNTPENFEAIAHTYSLVLLVARSKNSSHEALIQSFQLAFSLRSISLNENVKLQASRRRSLFTLATSMIVFTSKAYNILSLISIAKMALTDKTVDPFLQLVNDSKLQSVADTVRQPSKAYGSKEDDEDALKALSSIKITESQSNESFATMIVQSLGKPANESSVLKERLLSNFTPDDACPLGVQLSLDTTGYQSGLKDDKHSDMVDIPLFTIDDDIPASGLESQASTDAQQQPLENLSLLSVDDILGSVLETTHHVGRISVSTPSNMPYKEMALHCENLLAGKQQKISTFMGAQSLLANSFRIPLPDYNQEKDESTNSNVQPSLPLLQSGNPFLDSNLGAPSPTTLPETGPMLCATAYQQQAAFFQLPASRPYDNFLKAAGC