AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025598

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025598.t1 MTR_4g039320 97.355 983 25 1 1 982 46 1028 0.0 1972
MS.gene025598.t1 MTR_7g091780 50.642 1013 397 18 1 982 50 990 0.0 900
MS.gene025598.t1 MTR_4g067110 43.825 1004 462 21 3 982 48 973 0.0 759
MS.gene025598.t1 MTR_2g098850 42.928 1011 457 17 3 982 48 969 0.0 746
MS.gene025598.t1 MTR_2g098850 54.565 471 195 2 3 454 48 518 0.0 550
MS.gene025598.t1 MTR_2g098850 38.702 416 228 10 574 982 554 949 1.78e-62 229
MS.gene025598.t1 MTR_1g084090 32.754 1035 560 27 1 982 46 997 1.40e-153 480
MS.gene025598.t1 MTR_1g082810 53.261 92 43 0 1 92 8 99 2.06e-27 107
MS.gene025598.t1 MTR_1g082815 46.875 64 34 0 99 162 1 64 3.45e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025598.t1 AT5G21080 55.424 1014 376 19 1 979 46 1018 0.0 1017
MS.gene025598.t1 AT2G41830 49.756 1023 426 13 1 982 50 1025 0.0 939
MS.gene025598.t1 AT2G41830 48.559 937 394 13 87 982 1 890 0.0 813
MS.gene025598.t1 AT1G05960 49.460 463 215 4 1 448 46 504 6.04e-150 470
MS.gene025598.t1 AT1G05960 36.730 422 226 13 575 982 588 982 8.83e-57 213
MS.gene025598.t1 AT1G05960 49.460 463 215 4 1 448 46 504 6.04e-150 470
MS.gene025598.t1 AT1G05960 36.730 422 226 13 575 982 588 982 8.83e-57 213
MS.gene025598.t1 AT1G05960 49.460 463 215 4 1 448 46 504 6.04e-150 470
MS.gene025598.t1 AT1G05960 36.730 422 226 13 575 982 588 982 8.83e-57 213
MS.gene025598.t1 AT5G26850 32.609 1012 568 27 4 981 49 980 1.46e-148 466
MS.gene025598.t1 AT5G26850 32.609 1012 568 27 4 981 49 980 1.46e-148 466
MS.gene025598.t1 AT5G26850 32.609 1012 568 27 4 981 49 980 1.46e-148 466
MS.gene025598.t1 AT5G26850 32.609 1012 568 27 4 981 49 980 1.46e-148 466
MS.gene025598.t1 AT1G05960 47.107 484 216 5 1 448 46 525 1.63e-144 456
MS.gene025598.t1 AT1G05960 36.730 422 226 13 575 982 609 1003 1.07e-56 213

Find 204 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGCACTCTTTGAGAAAT+TGG 0.142400 4.3:-61527925 MS.gene025598:CDS
GATGATGACATTCCCGTTTC+TGG 0.176627 4.3:-61525543 MS.gene025598:CDS
GCTGAGTGTTGATGACATTT+TGG 0.183306 4.3:-61525463 MS.gene025598:CDS
ACAATATCAAGTTGCATATT+AGG 0.203881 4.3:+61528743 None:intergenic
GGAAATCTTCTGTTGCTTTC+CGG 0.219003 4.3:+61525158 None:intergenic
ATCAAGGAAGATTCTCAAAA+TGG 0.237376 4.3:-61529270 MS.gene025598:CDS
TGGAGAGCCATTTCTTTATA+TGG 0.242690 4.3:+61525198 None:intergenic
TAAATGTTTGTTACATTTGC+AGG 0.259482 4.3:+61525886 None:intergenic
TTCAATCTATTCATCATAGA+AGG 0.267853 4.3:+61527750 None:intergenic
ATGCCACGAACCCTGGATTT+TGG 0.270130 4.3:-61529095 MS.gene025598:CDS
TCAGGTTGTTTCGGTTGTTT+TGG 0.277392 4.3:-61529307 MS.gene025598:intron
CATTACTTGCCAATTCATTC+AGG 0.289885 4.3:-61525116 MS.gene025598:CDS
GGATGATGAGGACGCTTTAA+AGG 0.298782 4.3:-61525986 MS.gene025598:CDS
TGTTTCACAGGTTTGGTTCA+TGG 0.302837 4.3:-61529900 MS.gene025598:intron
AAGAGTTTGCATACTAAATA+TGG 0.304713 4.3:-61529070 MS.gene025598:CDS
CCTCGTACAGTTAACAATTA+AGG 0.308024 4.3:-61528540 MS.gene025598:intron
TTGCTGATCTTACGATCATT+AGG 0.317253 4.3:+61531760 None:intergenic
ATCAAGGTGCTTGACTAAAA+TGG 0.317663 4.3:+61528787 None:intergenic
TGACATTCCCGTTTCTGGTT+TGG 0.328957 4.3:-61525538 MS.gene025598:CDS
CTCCCATTTGTTGAGCTAGA+TGG 0.335829 4.3:+61530115 None:intergenic
ACAGAAGATTTCCACTTTCA+TGG 0.338277 4.3:-61525148 MS.gene025598:CDS
AGCATCTTCTTCAACAATTA+CGG 0.339473 4.3:+61527683 None:intergenic
TCATCATCAATAGTAAATAA+TGG 0.344171 4.3:+61525558 None:intergenic
AAGTTGTCATTTGTATTTAT+AGG 0.344443 4.3:-61531186 MS.gene025598:CDS
GGTTGATCCTTTCCTGCAAC+TGG 0.351301 4.3:-61526079 MS.gene025598:CDS
GGATGATAAACATTCTGATA+TGG 0.355282 4.3:-61525686 MS.gene025598:intron
GTACAGCATTTGCGAGCTTC+CGG 0.358129 4.3:-61530081 MS.gene025598:CDS
ATTGAGAGAAATGCTCCTTA+AGG 0.361727 4.3:+61526858 None:intergenic
TGCTGTCATTAACCAGTTGC+AGG 0.367595 4.3:+61526067 None:intergenic
TCTTGAGCAAAGATGTTATA+AGG 0.374255 4.3:-61531239 MS.gene025598:CDS
CGATTCATTTGACTGGCTTT+CGG 0.377099 4.3:+61525942 None:intergenic
TCTCTTGATGATTCGAACTT+AGG 0.380277 4.3:-61528614 MS.gene025598:CDS
GTGCACCCACCCTGGTTTCA+TGG 0.382038 4.3:+61528078 None:intergenic
GATAGGGCAAGCCCGTGTTC+TGG 0.382269 4.3:+61528964 None:intergenic
TAAAACACTATCTTCTTGTA+TGG 0.383577 4.3:+61527887 None:intergenic
TCTCATTTCTATCGCAAAAC+TGG 0.383874 4.3:-61526209 MS.gene025598:CDS
GCAGAGTGGAAATCCATTCT+TGG 0.384260 4.3:-61524928 MS.gene025598:CDS
CCATTCTTGGATTCAAACTT+AGG 0.385208 4.3:-61524915 MS.gene025598:CDS
TTAAGACATTCTTGTGATCA+AGG 0.387178 4.3:+61528771 None:intergenic
GTTTCACAGGTTTGGTTCAT+GGG 0.388563 4.3:-61529899 MS.gene025598:intron
ATTAACAACCAGCAGCCTTC+AGG 0.389547 4.3:+61524781 None:intergenic
CCCAAATTGATGATAGCAAA+AGG 0.403707 4.3:+61527528 None:intergenic
CTTGTTAACTCAGGTTGTTT+CGG 0.407813 4.3:-61529316 MS.gene025598:intron
GCATGTCGAGCAAGACAGAT+AGG 0.409849 4.3:+61528947 None:intergenic
AGGCAGCCAAGTAAAGCTTA+TGG 0.410878 4.3:-61526018 MS.gene025598:CDS
TCACAAAGTCAAACAAGGTT+TGG 0.411915 4.3:+61530331 None:intergenic
CCGGATGATGCCTGTCCATT+GGG 0.421408 4.3:-61525754 MS.gene025598:CDS
TGTTCAGCCAAGTTTGCCTT+TGG 0.422796 4.3:-61525043 MS.gene025598:intron
TGGCTGAACATTGGAATTTG+TGG 0.423136 4.3:+61525056 None:intergenic
CTCTTGATGATTCGAACTTA+GGG 0.423225 4.3:-61528613 MS.gene025598:CDS
TTCTTCTTCAAATCCTCCCT+TGG 0.423494 4.3:-61528006 MS.gene025598:CDS
TCTTTATATGGCATGTTTGA+AGG 0.424023 4.3:+61525210 None:intergenic
AACTGATGAAATAATGCCTC+AGG 0.426761 4.3:+61528125 None:intergenic
AAGAGGAATCCTGAATGAAT+TGG 0.426773 4.3:+61525107 None:intergenic
CAAACTCTTGACCAAAATCC+AGG 0.427241 4.3:+61529084 None:intergenic
CAGCAATCGGTGGCTATAAT+CGG 0.431344 4.3:-61528683 MS.gene025598:CDS
CTTACTAGTTGCTCGGAGTA+AGG 0.431485 4.3:-61527438 MS.gene025598:intron
AGGCACGTTGAGTTCTCCTT+TGG 0.438517 4.3:+61529209 None:intergenic
AAACATGCCATATAAAGAAA+TGG 0.438665 4.3:-61525205 MS.gene025598:CDS
TTGCCATCTAGCTCAACAAA+TGG 0.438712 4.3:-61530118 MS.gene025598:CDS
TTGTTCTGATACGACAAATT+CGG 0.439720 4.3:+61528253 None:intergenic
CAAAGCATTGGACCGGTCTC+AGG 0.441829 4.3:+61524870 None:intergenic
AGCAGTACCAAAGGCAAACT+TGG 0.442672 4.3:+61525036 None:intergenic
CTGTTGTATCAAGAGATAAC+TGG 0.442960 4.3:+61525728 None:intergenic
CCCTGGTTTCATGGTCTGCA+TGG 0.444341 4.3:+61528087 None:intergenic
TGGCTTTCCAAACCAGAAAC+GGG 0.446194 4.3:+61525531 None:intergenic
AGAAGGGTTATTAACCTTTG+TGG 0.446634 4.3:+61527767 None:intergenic
TCTATAAACTGCAGCAATCA+AGG 0.447846 4.3:+61528300 None:intergenic
TTTGATCCATAAGCTTTACT+TGG 0.449616 4.3:+61526012 None:intergenic
TTGGCTTTCCAAACCAGAAA+CGG 0.450173 4.3:+61525530 None:intergenic
AATCTGTTGCTGATACAGTT+AGG 0.454028 4.3:-61526038 MS.gene025598:CDS
TGATGACTACTCAGCCTAAT+CGG 0.455645 4.3:+61527557 None:intergenic
TACGCGTTATTCGTGGAGAA+TGG 0.458427 4.3:-61529241 MS.gene025598:CDS
GTTTGACTTTGTGAACAATC+AGG 0.459893 4.3:-61530322 MS.gene025598:intron
CAGAAGATTTCCACTTTCAT+GGG 0.461514 4.3:-61525147 MS.gene025598:CDS
AGAGAGGATGTTGTAGGCCT+TGG 0.462324 4.3:+61526231 None:intergenic
CATGTCTAATATTACAGTTA+TGG 0.464441 4.3:-61528329 MS.gene025598:CDS
TTATCATATGGACGCGATGC+TGG 0.465130 4.3:+61524807 None:intergenic
CCTAAGTTTGAATCCAAGAA+TGG 0.465692 4.3:+61524915 None:intergenic
GGAAAACTATGGGGATATCA+AGG 0.467639 4.3:-61529286 MS.gene025598:CDS
CCCAATGGACAGGCATCATC+CGG 0.468273 4.3:+61525754 None:intergenic
TGCCACAGCTTATCAGCAGC+AGG 0.469495 4.3:-61524847 MS.gene025598:CDS
AATGAGAGAGAGGATGTTGT+AGG 0.471509 4.3:+61526225 None:intergenic
TACAACTGTACGTCGTGTCC+TGG 0.473240 4.3:-61529028 MS.gene025598:CDS
CTCAAAGAGTGCAGCTGAAG+AGG 0.473402 4.3:+61527932 None:intergenic
AGGGAAGATGCCACGAACCC+TGG 0.476421 4.3:-61529102 MS.gene025598:CDS
TCAAGGAAGATTCTCAAAAT+GGG 0.477988 4.3:-61529269 MS.gene025598:CDS
GCTCAACAAATGGGAGATGA+TGG 0.478655 4.3:-61530108 MS.gene025598:CDS
ACCGGATGATGCCTGTCCAT+TGG 0.479159 4.3:-61525755 MS.gene025598:CDS
TGTCACAGGTATTACCGATT+AGG 0.479604 4.3:-61527571 MS.gene025598:intron
GATAATTTCCTGAAGGCTGC+TGG 0.482284 4.3:-61524789 MS.gene025598:CDS
CAGTTATCTCTTGATACAAC+AGG 0.483279 4.3:-61525727 MS.gene025598:CDS
ATGAATTGGCAAGTAATGAT+TGG 0.490570 4.3:+61525121 None:intergenic
GGATTTGAAGAAGAAGGTTG+AGG 0.490876 4.3:+61528014 None:intergenic
AATAGCGGAACAAGGATTCC+AGG 0.490919 4.3:+61529010 None:intergenic
GGAATTCTACGCGTTATTCG+TGG 0.491050 4.3:-61529248 MS.gene025598:CDS
ATTATTTCATCAGTTACTCC+TGG 0.493629 4.3:-61528117 MS.gene025598:CDS
TCCATGCAGACCATGAAACC+AGG 0.494159 4.3:-61528088 MS.gene025598:CDS
CAATCTATTATTGAAAATGC+TGG 0.494952 4.3:-61528925 MS.gene025598:intron
GCCTTCAGGAAATTATCATA+TGG 0.495284 4.3:+61524795 None:intergenic
ACGTACAGTTGTACCTTCCT+TGG 0.497866 4.3:+61529038 None:intergenic
TCAGGCAATGTGGTGGGTGA+CGG 0.498916 4.3:+61524888 None:intergenic
AAATCCTCTTCGTGTTCCTA+AGG 0.499125 4.3:-61531716 MS.gene025598:intron
GTTGTGCATCGGTACTAGCT+TGG 0.499618 4.3:+61525511 None:intergenic
ACGGCCATGGTATCGAGAAC+AGG 0.501724 4.3:+61528364 None:intergenic
CCCACCACATTGCCTGAGAC+CGG 0.501838 4.3:-61524882 MS.gene025598:CDS
TTGTATTTATAGGAAGTTGT+TGG 0.503668 4.3:-61531176 MS.gene025598:CDS
GGTCTGCATGGACCATTGCC+AGG 0.503771 4.3:+61528099 None:intergenic
TTGGTTCCATTGTATTACTT+CGG 0.504659 4.3:+61528589 None:intergenic
GATTCAGTGCTCGTCAAAGC+AGG 0.507356 4.3:+61527789 None:intergenic
TTCAAATCCTCCCTTGGCAA+AGG 0.508274 4.3:-61528000 MS.gene025598:CDS
AGGATACCAGTCTGGACTAA+AGG 0.508989 4.3:-61525707 MS.gene025598:CDS
AGCATTTCTCTCAATGAAAA+CGG 0.509668 4.3:-61526850 MS.gene025598:intron
AAGGGAGGATTTGAAGAAGA+AGG 0.510924 4.3:+61528008 None:intergenic
TAAAGGCTGTTGTTGTGCAT+CGG 0.510975 4.3:+61525500 None:intergenic
CGATGCTGGCAGTTGAAAGA+AGG 0.515201 4.3:+61524821 None:intergenic
TATCATCCTTTAGTCCAGAC+TGG 0.515638 4.3:+61525701 None:intergenic
TAGCAAACGATTCATTTGAC+TGG 0.518116 4.3:+61525935 None:intergenic
TCTTGGTTGTAATCAGGAAG+AGG 0.518222 4.3:+61525090 None:intergenic
GCCTTGTCTTACTAGTTGCT+CGG 0.519811 4.3:-61527445 MS.gene025598:CDS
TGACCAAAATCCAGGGTTCG+TGG 0.523303 4.3:+61529092 None:intergenic
GCCTCTTCACACTAGTTACT+CGG 0.527178 4.3:+61527715 None:intergenic
TCATTAACCAGTTGCAGGAA+AGG 0.527548 4.3:+61526072 None:intergenic
TCCTGATTACAACCAAGAAA+AGG 0.529816 4.3:-61525085 MS.gene025598:CDS
TGCCATCTAGCTCAACAAAT+GGG 0.530176 4.3:-61530117 MS.gene025598:CDS
GATACAACAGGATACCAGTC+TGG 0.531614 4.3:-61525715 MS.gene025598:CDS
AATATGCAACTTGATATTGT+CGG 0.532526 4.3:-61528740 MS.gene025598:CDS
ACTTCAAGTCCTATCGTCAA+TGG 0.534321 4.3:-61530058 MS.gene025598:intron
TTGCGATAGAAATGAGAGAG+AGG 0.539350 4.3:+61526215 None:intergenic
TGAATTGGCAAGTAATGATT+GGG 0.541834 4.3:+61525122 None:intergenic
AGGCAAACTTGGCTGAACAT+TGG 0.542103 4.3:+61525047 None:intergenic
ACCGGTCTCAGGCAATGTGG+TGG 0.544210 4.3:+61524881 None:intergenic
TGACGATAGGACTTGAAGTC+CGG 0.545617 4.3:+61530062 None:intergenic
TAAGCTGTGGCACAAAGCAT+TGG 0.545776 4.3:+61524858 None:intergenic
AAATGTCATCAACACTCAGC+AGG 0.545797 4.3:+61525466 None:intergenic
TCATCAGTTACTCCTGGCAA+TGG 0.545940 4.3:-61528111 MS.gene025598:CDS
CCTGCAGAATTCTTCTCATG+AGG 0.550469 4.3:-61526911 MS.gene025598:intron
CCAAATTGATGATAGCAAAA+GGG 0.553351 4.3:+61527529 None:intergenic
AATATCAGTTGCCTTTGCCA+AGG 0.555412 4.3:+61527989 None:intergenic
TGATTGGGCACCCATGAAAG+TGG 0.559105 4.3:+61525137 None:intergenic
TCTTCTGTTGCTTTCCGGCG+AGG 0.560458 4.3:+61525163 None:intergenic
TAAAGCTTATGGATCAAAAG+AGG 0.560884 4.3:-61526007 MS.gene025598:CDS
ACTATGATAGTGCAGAGTCT+AGG 0.562791 4.3:-61525913 MS.gene025598:CDS
CTTGGATGTGAAAACAATCA+TGG 0.566220 4.3:+61526249 None:intergenic
CATTGTGAGAACCTCCTCGC+CGG 0.567146 4.3:-61525177 MS.gene025598:CDS
CGGCGAGGAGGTTCTCACAA+TGG 0.570303 4.3:+61525178 None:intergenic
AGCAAGCTACTTGCAAACAA+AGG 0.572591 4.3:+61530414 None:intergenic
CCGGTCTCAGGCAATGTGGT+GGG 0.575594 4.3:+61524882 None:intergenic
TACGAGGCATTCATCTACTT+CGG 0.575702 4.3:+61528556 None:intergenic
GGCCTGCTGCTGATAAGCTG+TGG 0.575807 4.3:+61524845 None:intergenic
AGACAACACACCACGTAGGA+AGG 0.575883 4.3:-61525248 MS.gene025598:CDS
TGACTTCAATCTATTAAGAA+CGG 0.576842 4.3:+61527842 None:intergenic
AACTGCAAGCATCACGCCGA+AGG 0.577152 4.3:-61526294 MS.gene025598:CDS
ATATCAGTTGCCTTTGCCAA+GGG 0.577247 4.3:+61527990 None:intergenic
AGAGATAACTGGACTCCCAA+TGG 0.578004 4.3:+61525739 None:intergenic
TCAATCTATTCATCATAGAA+GGG 0.579432 4.3:+61527751 None:intergenic
GTTATGCAGATCTCAGATGC+AGG 0.580061 4.3:-61528388 MS.gene025598:intron
GTGTTCTGGAGACCAGAGAT+TGG 0.580951 4.3:+61528978 None:intergenic
AAAGATGTTATAAGGAGTTG+AGG 0.581387 4.3:-61531231 MS.gene025598:CDS
TAACTGGACTCCCAATGGAC+AGG 0.582987 4.3:+61525744 None:intergenic
CCTTAATTGTTAACTGTACG+AGG 0.582996 4.3:+61528540 None:intergenic
CATGTCGAGCAAGACAGATA+GGG 0.585911 4.3:+61528948 None:intergenic
TGTGGCACAAAGCATTGGAC+CGG 0.594211 4.3:+61524863 None:intergenic
ATGGCTAAGTTAGCCAAGGA+AGG 0.594419 4.3:-61529051 MS.gene025598:CDS
AATCTCTGGTCTCCAGAACA+CGG 0.597119 4.3:-61528976 MS.gene025598:CDS
TCAGTTGCCTTTGCCAAGGG+AGG 0.598902 4.3:+61527993 None:intergenic
TGCTGGCAGTTGAAAGAAGG+CGG 0.602146 4.3:+61524824 None:intergenic
TGGATCAAAAGAGGATGATG+AGG 0.602970 4.3:-61525998 MS.gene025598:CDS
GACAAACTGACGATGGCACA+AGG 0.608467 4.3:+61528036 None:intergenic
GCCGAGTAACTAGTGTGAAG+AGG 0.610135 4.3:-61527716 MS.gene025598:CDS
TATGCGATGTGCACCCACCC+TGG 0.610716 4.3:+61528070 None:intergenic
AAATATGGCTAAGTTAGCCA+AGG 0.613203 4.3:-61529055 MS.gene025598:CDS
TCCATATGATAATTTCCTGA+AGG 0.615364 4.3:-61524796 MS.gene025598:CDS
ATCTCTGGTCTCCAGAACAC+GGG 0.621594 4.3:-61528975 MS.gene025598:CDS
TCTGTTGCTTTCCGGCGAGG+AGG 0.621680 4.3:+61525166 None:intergenic
CTAGTGTAAAGAGAGACCTT+CGG 0.623227 4.3:+61526278 None:intergenic
AATCGTCCGTGTTAAAAGAA+CGG 0.624415 4.3:-61525795 MS.gene025598:CDS
ATTGTTCACAAAGTCAAACA+AGG 0.626698 4.3:+61530326 None:intergenic
AAATTCTTCTTGATCAATCG+CGG 0.626834 4.3:-61530380 MS.gene025598:CDS
TCAACAATTACGGATACTTG+AGG 0.628038 4.3:+61527693 None:intergenic
ATTATCAAAATAGCGGAACA+AGG 0.628643 4.3:+61529002 None:intergenic
GATTGAAGTCAAGTTATAGT+CGG 0.629009 4.3:-61527830 MS.gene025598:CDS
ACTGATGAAATAATGCCTCA+GGG 0.635325 4.3:+61528126 None:intergenic
AAACTCTTGACCAAAATCCA+GGG 0.643801 4.3:+61529085 None:intergenic
GATTGGTATTATCAAAATAG+CGG 0.647821 4.3:+61528995 None:intergenic
AGGTCCTGTTCTCGATACCA+TGG 0.653165 4.3:-61528368 MS.gene025598:CDS
TAGGGTCCGAAGTAATACAA+TGG 0.653776 4.3:-61528595 MS.gene025598:CDS
ATCATATTACCATTGACGAT+AGG 0.657452 4.3:+61530049 None:intergenic
AACTAGTAAGACAAGGCTGT+AGG 0.657522 4.3:+61527451 None:intergenic
AACAAGAGTTCAGCAATCGG+TGG 0.657645 4.3:-61528693 MS.gene025598:CDS
GAATTTGTCGTATCAGAACA+AGG 0.659033 4.3:-61528251 MS.gene025598:intron
TGGACCGGTCTCAGGCAATG+TGG 0.668100 4.3:+61524878 None:intergenic
GCAGACCATGAAACCAGGGT+GGG 0.677610 4.3:-61528083 MS.gene025598:CDS
CCATGCAGACCATGAAACCA+GGG 0.679392 4.3:-61528087 MS.gene025598:CDS
CATACCTTAGGAACACGAAG+AGG 0.681157 4.3:+61531712 None:intergenic
TGCAGACCATGAAACCAGGG+TGG 0.685737 4.3:-61528084 MS.gene025598:CDS
AGAATGGTAGTGAATGCCAA+AGG 0.687586 4.3:-61529225 MS.gene025598:CDS
TCCGAGCAACTAGTAAGACA+AGG 0.696577 4.3:+61527444 None:intergenic
GACGAGCACTGAATCCACAA+AGG 0.700276 4.3:-61527781 MS.gene025598:CDS
GGTTGAGGACAAACTGACGA+TGG 0.710132 4.3:+61528029 None:intergenic
GTGATATGATGAGACACTTG+CGG 0.716325 4.3:-61528652 MS.gene025598:CDS
TTGGAGACAACACACCACGT+AGG 0.734551 4.3:-61525252 MS.gene025598:CDS
TATTCTTTAGGCATTCCCTG+AGG 0.740034 4.3:-61528141 MS.gene025598:intron
AAACAGAAATCCTTCCTACG+TGG 0.759461 4.3:+61525238 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.3 gene 61524782 61531791 61524782 ID=MS.gene025598
chr4.3 mRNA 61524782 61531791 61524782 ID=MS.gene025598.t1;Parent=MS.gene025598
chr4.3 exon 61531717 61531791 61531717 ID=MS.gene025598.t1.exon1;Parent=MS.gene025598.t1
chr4.3 CDS 61531717 61531791 61531717 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61531157 61531272 61531157 ID=MS.gene025598.t1.exon2;Parent=MS.gene025598.t1
chr4.3 CDS 61531157 61531272 61531157 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61530323 61530437 61530323 ID=MS.gene025598.t1.exon3;Parent=MS.gene025598.t1
chr4.3 CDS 61530323 61530437 61530323 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61530059 61530187 61530059 ID=MS.gene025598.t1.exon4;Parent=MS.gene025598.t1
chr4.3 CDS 61530059 61530187 61530059 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61529865 61529912 61529865 ID=MS.gene025598.t1.exon5;Parent=MS.gene025598.t1
chr4.3 CDS 61529865 61529912 61529865 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61529207 61529325 61529207 ID=MS.gene025598.t1.exon6;Parent=MS.gene025598.t1
chr4.3 CDS 61529207 61529325 61529207 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61528926 61529122 61528926 ID=MS.gene025598.t1.exon7;Parent=MS.gene025598.t1
chr4.3 CDS 61528926 61529122 61528926 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61528541 61528833 61528541 ID=MS.gene025598.t1.exon8;Parent=MS.gene025598.t1
chr4.3 CDS 61528541 61528833 61528541 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61528252 61528401 61528252 ID=MS.gene025598.t1.exon9;Parent=MS.gene025598.t1
chr4.3 CDS 61528252 61528401 61528252 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61527668 61528153 61527668 ID=MS.gene025598.t1.exon10;Parent=MS.gene025598.t1
chr4.3 CDS 61527668 61528153 61527668 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61527439 61527585 61527439 ID=MS.gene025598.t1.exon11;Parent=MS.gene025598.t1
chr4.3 CDS 61527439 61527585 61527439 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61526851 61526926 61526851 ID=MS.gene025598.t1.exon12;Parent=MS.gene025598.t1
chr4.3 CDS 61526851 61526926 61526851 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61526192 61526319 61526192 ID=MS.gene025598.t1.exon13;Parent=MS.gene025598.t1
chr4.3 CDS 61526192 61526319 61526192 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61525900 61526100 61525900 ID=MS.gene025598.t1.exon14;Parent=MS.gene025598.t1
chr4.3 CDS 61525900 61526100 61525900 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61525687 61525818 61525687 ID=MS.gene025598.t1.exon15;Parent=MS.gene025598.t1
chr4.3 CDS 61525687 61525818 61525687 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61525458 61525589 61525458 ID=MS.gene025598.t1.exon16;Parent=MS.gene025598.t1
chr4.3 CDS 61525458 61525589 61525458 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61525044 61525277 61525044 ID=MS.gene025598.t1.exon17;Parent=MS.gene025598.t1
chr4.3 CDS 61525044 61525277 61525044 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
chr4.3 exon 61524782 61524952 61524782 ID=MS.gene025598.t1.exon18;Parent=MS.gene025598.t1
chr4.3 CDS 61524782 61524952 61524782 ID=cds.MS.gene025598.t1;Parent=MS.gene025598.t1
Gene Sequence

>MS.gene025598

GAAGAAGAACCTAATGATCGTAAGATCAGCAAGTTATGCGAGTATGCATCGAAAAATCCTCTTCGTGTTCCTAAGGTATGTGATGAATTGTATTGATTGTAAAATGGAATTGGAATTGGAACTATAATTTTTATTTTTATTTTTATGAGCAAGACCCAATTCAGTCCCTCATAATTTTCTTGTGTTTCAATTCAATCCTTGACGAAAAAAATACCCAAATTACTCAACAACATTTTCATACTTCGGACAAATTAGTCCCTCTAACATAATGATACAGGGACTAATTTATCCTCGCACGGACTAATTTGAGTTTAATTTTTGTCTGGCACCGAATTGTGCCATTAGAAAATTGTGAGGAATCGAGTTGGGTTTTCACTCCTTGTTGAATAACCCTAGGGGCAATAATTGTAGTCACACTTATCACAACGCCACAAATCAAGAGTACCAAAATTGTTTTTTTTTTTTTTTTACTTACTGATATTTGTAAAATGACTGTGTTGCCCTTGGTTGTTTTTACAGATAACAAGCTATCTTGAGCAAAGATGTTATAAGGAGTTGAGGACTGAGAATTATCAAGCTGTGAAAGTTGTCATTTGTATTTATAGGAAGTTGTTGGTTTCTTGTAGAGATCAAATGTGAGTTCTTTTTTCATCATAGTTATCAGTATGTTGATATAATAAGTAGGGATCTTAGTTTGTAATTGCAAACTTAAAAATTAATAAAATTTGATGGTTAATTGATAGTCAGCATCATATGTTTTATCATCGTAAGTATCTAATTAGTATCCTATATGACTTGATGTGCTAATCAAGTGATTAGGATCAAGCGGTTAATGAGCTTCAATTAAGGAGATCGTTCGGAAGAACCCGAGTTCAAATCCTGACTGGAATAGTTATTGGTCAAACTTTACTTACATTCAGGCCGAACTCCGGATTATCAGGGCCCTCTCTCACTGGGAACCTGAGGATTAGGAATATTATGTGATGTTCTTTTAGTTTACATTATCATATATGAAGGAAATGTATTTATTCCTGACTGGAATAGTTATTGGTCAAACTTTACTTACCTTCAGGCCGAACTCCGGATTATCAGGGCCCTCTCTCACTGGGAACCCGAGGATTAGGAAAAAAATATTATGTGATGTTTTTTTAGTTTACATTATCATATATGAAGGAAATGTATTTATTTAGAAGTGTAAGAGCTTGGTTGTTTTATAGGCTTAGCTTAAGTTTACTTTGGTTTGCAACCATTTGAAAAACAGTAACCTAAGGAAACAACAGAAGCTGATTTTTCAATTTATTACGATTAAAACCATTTTTATTGCGTATGATTTATCAGAACACTATTGTGGCAGGCCTTTGTTTGCAAGTAGCTTGCTTAGCATCATTCAAATTCTTCTTGATCAATCGCGGCAAGACGAAGTGCAGATTTTAGGATGCCAAACCTTGTTTGACTTTGTGAACAATCAGGTGGGGAATTTTTGGTAGGTTCTCAGTTTATGAGTTTGAAGCTAGTTCACTCTTTACAAAGTAGTTAAATGTTTACAACATTGTATGTTTTTTTGCATGGAAGTTTGATCTTGTATCATTCTTTTATTTGTGCAGAGGGATGGTACTTATATGTTTAATTTAGACAGTTTCATTTCAAAACTTTGCCATCTAGCTCAACAAATGGGAGATGATGGAAAAGTACAGCATTTGCGAGCTTCCGGACTTCAAGTCCTATCGTCAATGGTAATATGATTTCTACTTTATCATGTCTGCGATATGGCTATCAAGTCAAACTGCAACAAATCCAATTGATGCGCCAAAGACCCCATTGAAAAAGGCTACTGTTATTGCTGATGTAAAATTGATAAGATTCTTTTCTTGTTTCACAGGTTTGGTTCATGGGAGAATTCACTCATATTTCAGTTGAATTTGACAATGTAAGTTATTGATCTGTTTACATTTTCCTTTAAGGTATTAGTCATTTCGCACGGAGAAAAGAACACATTAGTGCTTGATTTTAGTTTTTTGGTACTTCTCGAATCAGCGAACTTTCTTTGACCTCAAACTAGGTGTTTAGTGGAGAAATTCATCTTGTTTTTTTTTCTTCATGATTAACTTATTTTCGATAGAGGGGTAACCTTGGCGCAACTGGTAAAGTTGTTGTCATGTGACTAAAAGGTCACGGGTTCAGGTCCTGGAAACAACCTCTTGTGTAAAACAGGGTAAGGCTGCGTACAATACACCAAATGGTGGGACCCCTTCCCGGACCCTGCATATGCGGGAGCTCTAGTGCACTGGGTTGCCCTTTTTAACTTATTTTCGATAACTTTCATTATATTACATTCTGATTGTGAGAGATAAACACCTCTATTGACTAAAATCGTTTCCAAACATCGTATACCACTTCCAATTACCTCTTGTCTTACCCTCCCGTATACTTCTTTCATTTGACAGCTTGCATCTCTTGTTAACTCAGGTTGTTTCGGTTGTTTTGGAAAACTATGGGGATATCAAGGAAGATTCTCAAAATGGGAATTCTACGCGTTATTCGTGGAGAATGGTAGTGAATGCCAAAGGAGAACTCAACGTGCCTATGTAACTGTCCTTCTTGTTATATTAGCTTTTGTTTTGAGTTACTCGTATGTTATAATTGATGTTAACTTCATTTCTCCTCCATAGGGAAGATGCCACGAACCCTGGATTTTGGTCAAGAGTTTGCATACTAAATATGGCTAAGTTAGCCAAGGAAGGTACAACTGTACGTCGTGTCCTGGAATCCTTGTTCCGCTATTTTGATAATACCAATCTCTGGTCTCCAGAACACGGGCTTGCCCTATCTGTCTTGCTCGACATGCAATCTATTATTGAAAATGCTGGTATTTATTTTCTATAGTGCTTTGAGATCAGATTCCTGTCAATGTTTCTATATCCTATCTAAACAGTTTGAAATTTGGTTCGCAATTTACAGGACAGAATACACACTTGTTACTATCCATTTTAGTCAAGCACCTTGATCACAAGAATGTCTTAAAGAATCCTAATATGCAACTTGATATTGTCGGTGTTATTACCCATCTTGCCGAAAAAACAAGAGTTCAGCAATCGGTGGCTATAATCGGTGCATTGAGTGATATGATGAGACACTTGCGGAAAAGCATACACTGCTCTCTTGATGATTCGAACTTAGGGTCCGAAGTAATACAATGGAACCAAAAATATAGAACCGAAGTAGATGAATGCCTCGTACAGTTAACAATTAAGGTGACAAAAATTCCAACTTTTAAGATGAACATTTTAGTTAATAGTGTAACTGACCAGATAAGCATGTAGTTCTGGATGGCGATTTGGCTTTGAATCTTGGATATGTTAATTAATGGCTACTGGCTATAATGTTATGCAGATCTCAGATGCAGGTCCTGTTCTCGATACCATGGCCGTGTTGTTAGAAAACATGTCTAATATTACAGTTATGGCAAGAACCTTGATTGCTGCAGTTTATAGAACTTCTCAAATAGTTGCATCTATACCGAATTTGTCGTATCAGAACAAGGCAAGTTACTTAAATTTCCTGATTCGTAATAACTGTAATATTTCCTTACTTCTTTCTTTCTCATTTTTTCTGTGAACTTGAAAAACTCTATTCTTTAGGCATTCCCTGAGGCATTATTTCATCAGTTACTCCTGGCAATGGTCCATGCAGACCATGAAACCAGGGTGGGTGCACATCGCATATTTTCTCTTGTCCTTGTGCCATCGTCAGTTTGTCCTCAACCTTCTTCTTCAAATCCTCCCTTGGCAAAGGCAACTGATATTCAAAGAATGCTCTCGAGAAATGTCTCTGTATTTTCCTCTTCAGCTGCACTCTTTGAGAAATTGGAGAAGAAGCAACTTTCCATACAAGAAGATAGTGTTTTAGATGGAAAGAGCAATGATAGCTCCGTTCTTAATAGATTGAAGTCAAGTTATAGTCGGACCGCTAGTATAAGAAAACCTGCTTTGACGAGCACTGAATCCACAAAGGTTAATAACCCTTCTATGATGAATAGATTGAAGTCAAGTTACAGCCGAGTAACTAGTGTGAAGAGGCCTCAAGTATCCGTAATTGTTGAAGAAGATGCTACAACTAAAAAACAGGTATGTATTTGCACTATTTTACTTTACATTTTATTTTAATAACAATATAACTTCTCAATCAAACACCATCACCATGTCACAGGTATTACCGATTAGGCTGAGTAGTCATCAGATTACCCTTTTGCTATCATCAATTTGGGTTCAGTCAATCTATCCTCTGAATACACCTGAAAATTTCGAAGCAATTGCTCATACCTACAGCCTTGTCTTACTAGTTGCTCGGAGTAAGGTAATGTGAAAATTCTTGATATTTTACCATGTATACATTGAATGAGAAAATTGTTTTCTTGTATGGACTAGTTGACCGATTTTTAACACAGTCCTTTCAGTTTACCGACTTTCTGATAAAAGTTTGAAAATTTAACTTTTAACAGAATAAAAATCGCCTCATTAACATATATATTTTCCTATTGCGGTTATTTAATTTATTTCGGTTATTTCAATTTATTTGAGAATGATTCTTATACCTGCGTCCTTGGTGATAGATAGTGTAGATGATTATTCATAAAGTTGTCTTAAGATTAAAATCAACCTCAGATAATTTACTCATACAATAACTTGCAAGTTATATCAACGGTCAAAATCAAAGTTATAAATTTTAATGTTACTTCAAGAATTTCCATTTCCATAAAAATGTGACACACACACACACACACACACACAAAAGCATTCATATCTCACATATATTTTTAAGTTTATCTTTATCTTATCCTCATAACTTGTATGCTTTGGCTCCTGCAGAATTCTTCTCATGAGGCTCTAATTCAAAGTTTTCAGTTGGCATTTTCCTTAAGGAGCATTTCTCTCAATGAAAACGGTAGAGTCTCGAATCCATATTATTTAATGTTTGTATGTTACCATGTTTTTTGTAAGAATGACAGCTCAATATCCGCGACATATGATATGGATATCTTATGAACCCATGTTTAGAATCAAATGACTTTGTTCCTATTTTTTTCCTTCCAAGGGAAGGTTTAGTGCTAAGGAAAGAAAACTGATAGAGTACGGAAATTAAATGAAAGGAGGAATTTATTAAATTAAAAAGTAGACTAAGCTCTCACATACACGGAAAAAGTCTAATGCGGCGCATGATGTAATATACTTAACAGGGAAGTTTGGCAGTTAACATTTTCCATGATATACAAAATTCATCAGATTATTCCTTACATAATCGACCAAATAGAACTGTAGGTTATACAGCATATATTTTATTTATCATGGCATATGTTCTCATATTAGATACAGTTTATTCATGTTTATTTTATGTTGCTTTTTGATGAAATGGTTTAAAATTGCGAAGCATTGTTAAGGTGCCTAGTTTTGAAACACTTCTTCTTTCTTTTTTCAGTTAAACTGCAAGCATCACGCCGAAGGTCTCTCTTTACACTAGCAACATCCATGATTGTTTTCACATCCAAGGCCTACAACATCCTCTCTCTCATTTCTATCGCAAAACTGGCATTGACAGATAAAACTGTATGTACATCTTGTTTTGTACAATGTTTTGCATTTTATATAATAAAATCACTCCTTTGTATCTCTTATGGTTTTTTATGCATCTTTTCAGGTTGATCCTTTCCTGCAACTGGTTAATGACAGCAAGTTACAATCTGTTGCTGATACAGTTAGGCAGCCAAGTAAAGCTTATGGATCAAAAGAGGATGATGAGGACGCTTTAAAGGCACTTTCATCGATAAAAATAACCGAAAGCCAGTCAAATGAATCGTTTGCTACTATGATAGTGCAGAGTCTAGGAAAACCTGCAAATGTAACAAACATTTATTTATCATTTAATGTTTCGCTAGTTTATCAGGATCAATGTATTAACTTACATTTTTTTGTGCATCAGGAATCGTCCGTGTTAAAAGAACGGCTTCTTAGTAATTTTACACCGGATGATGCCTGTCCATTGGGAGTCCAGTTATCTCTTGATACAACAGGATACCAGTCTGGACTAAAGGATGATAAACATTCTGATATGGTAATTTGCACAGCTCTCCTCAGACGCATTTTTCGTTAAAAATAAAAAATATTTCTCGGATTTTAACCTGTATAGTCGCCTACATGAAATTATGCAGGTTGATATTCCATTATTTACTATTGATGATGACATTCCCGTTTCTGGTTTGGAAAGCCAAGCTAGTACCGATGCACAACAACAGCCTTTAGAAAATCTAAGCCTGCTGAGTGTTGATGACATTTTGGGTTCAGTAAGTTTTTCTGTTCATTGATATGTACCTACCGATAACATGTTTGATTTTATTTAAGCTATAGTGAAAAATGAAAAAACATTCTCTTAACGCTCATCCATTTATCTCTTCAATTTCCTCATCGAGTAATTCTAAAAATTCTAGAAAATGATGAGTCGTAAAATGATAATTCATATGCAGGTTTTGGAGACAACACACCACGTAGGAAGGATTTCTGTTTCAACACCTTCAAACATGCCATATAAAGAAATGGCTCTCCATTGTGAGAACCTCCTCGCCGGAAAGCAACAGAAGATTTCCACTTTCATGGGTGCCCAATCATTACTTGCCAATTCATTCAGGATTCCTCTTCCTGATTACAACCAAGAAAAGGACGAATCCACAAATTCCAATGTTCAGCCAAGTTTGCCTTTGGTACTGCTTTACTTTATTTACTTTCGAAATTCAGAACAAGTGTTTCTTCTTGCTTTGAGATATCTTATTCCTGTTTCCTGTGCCATTTTAGTTGCAGAGTGGAAATCCATTCTTGGATTCAAACTTAGGCGCTCCGTCACCCACCACATTGCCTGAGACCGGTCCAATGCTTTGTGCCACAGCTTATCAGCAGCAGGCCGCCTTCTTTCAACTGCCAGCATCGCGTCCATATGATAATTTCCTGAAGGCTGCTGGTTGTTAA
Protein sequence

>MS.gene025598.t1

EEEPNDRKISKLCEYASKNPLRVPKITSYLEQRCYKELRTENYQAVKVVICIYRKLLVSCRDQMPLFASSLLSIIQILLDQSRQDEVQILGCQTLFDFVNNQRDGTYMFNLDSFISKLCHLAQQMGDDGKVQHLRASGLQVLSSMVWFMGEFTHISVEFDNVVSVVLENYGDIKEDSQNGNSTRYSWRMVVNAKGELNVPMEDATNPGFWSRVCILNMAKLAKEGTTVRRVLESLFRYFDNTNLWSPEHGLALSVLLDMQSIIENAGQNTHLLLSILVKHLDHKNVLKNPNMQLDIVGVITHLAEKTRVQQSVAIIGALSDMMRHLRKSIHCSLDDSNLGSEVIQWNQKYRTEVDECLVQLTIKISDAGPVLDTMAVLLENMSNITVMARTLIAAVYRTSQIVASIPNLSYQNKAFPEALFHQLLLAMVHADHETRVGAHRIFSLVLVPSSVCPQPSSSNPPLAKATDIQRMLSRNVSVFSSSAALFEKLEKKQLSIQEDSVLDGKSNDSSVLNRLKSSYSRTASIRKPALTSTESTKVNNPSMMNRLKSSYSRVTSVKRPQVSVIVEEDATTKKQVLPIRLSSHQITLLLSSIWVQSIYPLNTPENFEAIAHTYSLVLLVARSKNSSHEALIQSFQLAFSLRSISLNENVKLQASRRRSLFTLATSMIVFTSKAYNILSLISIAKLALTDKTVDPFLQLVNDSKLQSVADTVRQPSKAYGSKEDDEDALKALSSIKITESQSNESFATMIVQSLGKPANESSVLKERLLSNFTPDDACPLGVQLSLDTTGYQSGLKDDKHSDMVDIPLFTIDDDIPVSGLESQASTDAQQQPLENLSLLSVDDILGSVLETTHHVGRISVSTPSNMPYKEMALHCENLLAGKQQKISTFMGAQSLLANSFRIPLPDYNQEKDESTNSNVQPSLPLLQSGNPFLDSNLGAPSPTTLPETGPMLCATAYQQQAAFFQLPASRPYDNFLKAAGC