Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85537.t1 | AES68846.1 | 59.3 | 86 | 14 | 3 | 1 | 86 | 1 | 65 | 1.20E-12 | 83.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85537.t1 | G7IYS7 | 59.3 | 86 | 14 | 3 | 1 | 86 | 1 | 65 | 8.8e-13 | 83.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055356 | MS.gene85537 | 0.811673 | 6.23E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene85537 | 0.857583 | 1.48E-62 | -1.69E-46 |
| MS.gene058426 | MS.gene85537 | 0.802124 | 6.51E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85537.t1 | MTR_3g016080 | 60.563 | 71 | 6 | 2 | 1 | 69 | 1 | 51 | 1.64e-17 | 72.8 |
| MS.gene85537.t1 | MTR_3g014608 | 47.788 | 113 | 15 | 5 | 1 | 111 | 1 | 71 | 5.41e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTAAGGTGTTATCTTCTTT+TGG | 0.201728 | 3.4:+11463802 | None:intergenic |
| GGTAAGGTGTTATCTTCTTT+TGG | 0.201728 | 3.4:+11463874 | None:intergenic |
| GGTAAGGTGTTATCTTCTTT+TGG | 0.201728 | 3.4:+11463946 | None:intergenic |
| AAATGAGTGTTATCTTCTTT+TGG | 0.250224 | 3.4:+11463730 | None:intergenic |
| GGGTGTTGAGGAGGCTTTCT+TGG | 0.292723 | 3.4:+11463898 | None:intergenic |
| GGGTGTTGAGGAGGCTTTCT+TGG | 0.315739 | 3.4:+11463826 | None:intergenic |
| ATTCTCATCCCTCAAGGCTT+TGG | 0.330550 | 3.4:-11464141 | MS.gene85537:CDS |
| TTTGGGTTGGGTTGGTTGGT+AGG | 0.340770 | 3.4:+11463991 | None:intergenic |
| ACGGTGGTTTGTGTTTATTA+TGG | 0.357596 | 3.4:+11464103 | None:intergenic |
| GCTGGCAGGGTAAGTTGGTT+TGG | 0.372773 | 3.4:+11463780 | None:intergenic |
| GCTGGCAGGGTAAGTTGGTT+TGG | 0.372773 | 3.4:+11463852 | None:intergenic |
| GCTGGCAGGGTAAGTTGGTT+TGG | 0.375568 | 3.4:+11463924 | None:intergenic |
| GGTTGGTTGGTAGGATGTAT+AGG | 0.386912 | 3.4:+11464000 | None:intergenic |
| CCACCATCTACTCAAGGATT+TGG | 0.393716 | 3.4:-11464030 | MS.gene85537:CDS |
| TGAGTAGATGGTGGATGTTT+AGG | 0.399624 | 3.4:+11464039 | None:intergenic |
| AGGGTAAGTTGGTTTGGGTA+AGG | 0.401305 | 3.4:+11463786 | None:intergenic |
| AGGGTAAGTTGGTTTGGGTA+AGG | 0.401305 | 3.4:+11463858 | None:intergenic |
| AGGGTAAGTTGGTTTGGGTA+AGG | 0.401305 | 3.4:+11463930 | None:intergenic |
| CTGGCAGGGTAAGTTGGTTT+GGG | 0.411964 | 3.4:+11463781 | None:intergenic |
| CTGGCAGGGTAAGTTGGTTT+GGG | 0.411964 | 3.4:+11463853 | None:intergenic |
| CTGGCAGGGTAAGTTGGTTT+GGG | 0.411964 | 3.4:+11463925 | None:intergenic |
| GGCTTTCTTGGAATGCTGGC+AGG | 0.413646 | 3.4:+11463910 | None:intergenic |
| GGCTTTCTTGGAAAGCTGGC+AGG | 0.423521 | 3.4:+11463838 | None:intergenic |
| TCACCAAATCCTTGAGTAGA+TGG | 0.432255 | 3.4:+11464027 | None:intergenic |
| AGGAGGCTTTCTTGGAATGC+TGG | 0.435548 | 3.4:+11463906 | None:intergenic |
| AGGAGGCTTTCTTGGAAAGC+TGG | 0.454598 | 3.4:+11463834 | None:intergenic |
| GGTTTGTGTTTATTATGGTT+TGG | 0.471548 | 3.4:+11464108 | None:intergenic |
| GTTGAGCACCAAAGCCTTGA+GGG | 0.482178 | 3.4:+11464133 | None:intergenic |
| GGAAAGCTGGCAGGGTAAGT+TGG | 0.485288 | 3.4:+11463847 | None:intergenic |
| GGAAAGCTGGCAGGGTAAGT+TGG | 0.487771 | 3.4:+11463775 | None:intergenic |
| TCTCTTATTCTCATCCCTCA+AGG | 0.490859 | 3.4:-11464147 | MS.gene85537:CDS |
| GGAATGCTGGCAGGGTAAGT+TGG | 0.491701 | 3.4:+11463919 | None:intergenic |
| TTGTGTTTATTATGGTTTGG+AGG | 0.506608 | 3.4:+11464111 | None:intergenic |
| GCTTTCTTGGAATGCTGGCA+GGG | 0.564002 | 3.4:+11463911 | None:intergenic |
| CACAAACCACCGTCTACTCA+AGG | 0.569030 | 3.4:-11464093 | MS.gene85537:CDS |
| GCTTTCTTGGAAAGCTGGCA+GGG | 0.573877 | 3.4:+11463839 | None:intergenic |
| GGATGAGAATAAGAGAAGCA+AGG | 0.575636 | 3.4:+11464154 | None:intergenic |
| GAAGCAAATCCTTGAGTAGA+CGG | 0.588915 | 3.4:+11464084 | None:intergenic |
| AAACATCCACCATCTACTCA+AGG | 0.614124 | 3.4:-11464036 | MS.gene85537:CDS |
| CCAAATCCTTGAGTAGATGG+TGG | 0.614476 | 3.4:+11464030 | None:intergenic |
| GGTTGAGCACCAAAGCCTTG+AGG | 0.627683 | 3.4:+11464132 | None:intergenic |
| GAAAGACACAAGCAAGTACG+AGG | 0.644201 | 3.4:+11464176 | None:intergenic |
| GGATGTTTAGGATGATCATG+AGG | 0.681938 | 3.4:+11464051 | None:intergenic |
| GCAAATCCTTGAGTAGACGG+TGG | 0.700559 | 3.4:+11464087 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGAGTGTTATCTTCTTT+TGG | + | chr3.4:11464188-11464207 | None:intergenic | 25.0% |
| GGTTTGTGTTTATTATGGTT+TGG | + | chr3.4:11463810-11463829 | None:intergenic | 30.0% | |
| ! | TTGTGTTTATTATGGTTTGG+AGG | + | chr3.4:11463807-11463826 | None:intergenic | 30.0% |
| ! | ACGGTGGTTTGTGTTTATTA+TGG | + | chr3.4:11463815-11463834 | None:intergenic | 35.0% |
| ! | TGAGTGTTATCTTCTTTTGG+AGG | + | chr3.4:11464185-11464204 | None:intergenic | 35.0% |
| !! | AAGGTGTTATCTTCTTTTGG+AGG | + | chr3.4:11463969-11463988 | None:intergenic | 35.0% |
| !! | AGGTGTTATCTTCTTTTGGA+GGG | + | chr3.4:11463968-11463987 | None:intergenic | 35.0% |
| !! | GGTAAGGTGTTATCTTCTTT+TGG | + | chr3.4:11463972-11463991 | None:intergenic | 35.0% |
| AAACATCCACCATCTACTCA+AGG | - | chr3.4:11463879-11463898 | MS.gene85537:CDS | 40.0% | |
| GAAGCAAATCCTTGAGTAGA+CGG | + | chr3.4:11463834-11463853 | None:intergenic | 40.0% | |
| GGATGAGAATAAGAGAAGCA+AGG | + | chr3.4:11463764-11463783 | None:intergenic | 40.0% | |
| TCACCAAATCCTTGAGTAGA+TGG | + | chr3.4:11463891-11463910 | None:intergenic | 40.0% | |
| TCTCTTATTCTCATCCCTCA+AGG | - | chr3.4:11463768-11463787 | MS.gene85537:CDS | 40.0% | |
| ! | GGATGTTTAGGATGATCATG+AGG | + | chr3.4:11463867-11463886 | None:intergenic | 40.0% |
| !! | TGAGTAGATGGTGGATGTTT+AGG | + | chr3.4:11463879-11463898 | None:intergenic | 40.0% |
| !! | TGTTATCTTCTTTTGGAGGG+TGG | + | chr3.4:11463965-11463984 | None:intergenic | 40.0% |
| !!! | ACTTTTTTGGAAAGCTGGCA+GGG | + | chr3.4:11464151-11464170 | None:intergenic | 40.0% |
| !!! | AGGAGACTTTTTTGGAAAGC+TGG | + | chr3.4:11464156-11464175 | None:intergenic | 40.0% |
| !!! | AGGATCATGCTTTTTTGGGT+TGG | + | chr3.4:11463940-11463959 | None:intergenic | 40.0% |
| !!! | GGATCATGCTTTTTTGGGTT+GGG | + | chr3.4:11463939-11463958 | None:intergenic | 40.0% |
| !!! | GGTGAGGATCATGCTTTTTT+GGG | + | chr3.4:11463944-11463963 | None:intergenic | 40.0% |
| !!! | TGGTGAGGATCATGCTTTTT+TGG | + | chr3.4:11463945-11463964 | None:intergenic | 40.0% |
| ATTCTCATCCCTCAAGGCTT+TGG | - | chr3.4:11463774-11463793 | MS.gene85537:CDS | 45.0% | |
| CCAAATCCTTGAGTAGATGG+TGG | + | chr3.4:11463888-11463907 | None:intergenic | 45.0% | |
| CCACCATCTACTCAAGGATT+TGG | - | chr3.4:11463885-11463904 | MS.gene85537:CDS | 45.0% | |
| GAAAGACACAAGCAAGTACG+AGG | + | chr3.4:11463742-11463761 | None:intergenic | 45.0% | |
| !! | AGGGTAAGTTGGTTTGGGTA+AGG | + | chr3.4:11463988-11464007 | None:intergenic | 45.0% |
| !! | GGTTGGTTGGTAGGATGTAT+AGG | + | chr3.4:11463918-11463937 | None:intergenic | 45.0% |
| !! | TCTTCTTTTGGAGGCTGATG+AGG | + | chr3.4:11464176-11464195 | None:intergenic | 45.0% |
| !!! | CATGCTTTTTTGGGTTGGGT+TGG | + | chr3.4:11463935-11463954 | None:intergenic | 45.0% |
| !!! | CTTTTTTGGGTTGGGTTGGT+TGG | + | chr3.4:11463931-11463950 | None:intergenic | 45.0% |
| !!! | GACTTTTTTGGAAAGCTGGC+AGG | + | chr3.4:11464152-11464171 | None:intergenic | 45.0% |
| !!! | GGCTGATGAGGAGACTTTTT+TGG | + | chr3.4:11464164-11464183 | None:intergenic | 45.0% |
| !!! | TCTTCTTTTGGAGGGTGTTG+AGG | + | chr3.4:11464032-11464051 | None:intergenic | 45.0% |
| AGGAGGCTTTCTTGGAAAGC+TGG | + | chr3.4:11464084-11464103 | None:intergenic | 50.0% | |
| AGGAGGCTTTCTTGGAATGC+TGG | + | chr3.4:11464012-11464031 | None:intergenic | 50.0% | |
| CACAAACCACCGTCTACTCA+AGG | - | chr3.4:11463822-11463841 | MS.gene85537:CDS | 50.0% | |
| GCAAATCCTTGAGTAGACGG+TGG | + | chr3.4:11463831-11463850 | None:intergenic | 50.0% | |
| GCTTTCTTGGAAAGCTGGCA+GGG | + | chr3.4:11464079-11464098 | None:intergenic | 50.0% | |
| GCTTTCTTGGAATGCTGGCA+GGG | + | chr3.4:11464007-11464026 | None:intergenic | 50.0% | |
| GTTGAGCACCAAAGCCTTGA+GGG | + | chr3.4:11463785-11463804 | None:intergenic | 50.0% | |
| !! | CTGGCAGGGTAAGTTGGTTT+GGG | + | chr3.4:11463993-11464012 | None:intergenic | 50.0% |
| !! | TCTTCTTTTGGAGGGTGGTG+AGG | + | chr3.4:11463960-11463979 | None:intergenic | 50.0% |
| !! | TTTGGGTTGGGTTGGTTGGT+AGG | + | chr3.4:11463927-11463946 | None:intergenic | 50.0% |
| !!! | TCTTTTGGAGGGTGTTGAGG+AGG | + | chr3.4:11464029-11464048 | None:intergenic | 50.0% |
| GGAAAGCTGGCAGGGTAAGT+TGG | + | chr3.4:11464071-11464090 | None:intergenic | 55.0% | |
| GGAATGCTGGCAGGGTAAGT+TGG | + | chr3.4:11463999-11464018 | None:intergenic | 55.0% | |
| GGCTTTCTTGGAAAGCTGGC+AGG | + | chr3.4:11464080-11464099 | None:intergenic | 55.0% | |
| GGCTTTCTTGGAATGCTGGC+AGG | + | chr3.4:11464008-11464027 | None:intergenic | 55.0% | |
| GGTTGAGCACCAAAGCCTTG+AGG | + | chr3.4:11463786-11463805 | None:intergenic | 55.0% | |
| !! | GCTGGCAGGGTAAGTTGGTT+TGG | + | chr3.4:11463994-11464013 | None:intergenic | 55.0% |
| !! | GGGTGTTGAGGAGGCTTTCT+TGG | + | chr3.4:11464020-11464039 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 11463726 | 11464211 | 11463726 | ID=MS.gene85537 |
| chr3.4 | mRNA | 11463726 | 11464211 | 11463726 | ID=MS.gene85537.t1;Parent=MS.gene85537 |
| chr3.4 | exon | 11463726 | 11464211 | 11463726 | ID=MS.gene85537.t1.exon1;Parent=MS.gene85537.t1 |
| chr3.4 | CDS | 11463726 | 11464211 | 11463726 | ID=cds.MS.gene85537.t1;Parent=MS.gene85537.t1 |
| Gene Sequence |
| Protein sequence |
