AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85954

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85954.t1 MTR_2g104910 99.052 211 2 0 23 233 2 212 6.96e-154 426
MS.gene85954.t1 MTR_2g104910 99.052 211 2 0 23 233 13 223 2.16e-153 425
MS.gene85954.t1 MTR_4g006570 85.047 214 32 0 20 233 37 250 4.33e-132 372
MS.gene85954.t1 MTR_4g006570 85.782 211 30 0 23 233 15 225 5.29e-132 371
MS.gene85954.t1 MTR_4g006570 85.782 211 30 0 23 233 31 241 1.04e-131 371
MS.gene85954.t1 MTR_4g006570 85.782 211 30 0 23 233 45 255 1.71e-131 371
MS.gene85954.t1 MTR_4g006570 85.000 200 30 0 34 233 1 200 4.29e-124 350
MS.gene85954.t1 MTR_4g025850 36.771 223 126 7 23 233 21 240 1.88e-37 131
MS.gene85954.t1 MTR_4g025850 31.902 163 90 6 23 170 21 177 1.54e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85954.t1 AT1G15880 79.147 211 43 1 23 233 14 223 3.77e-118 336
MS.gene85954.t1 AT2G45200 36.283 226 123 5 23 233 20 239 5.97e-37 130
MS.gene85954.t1 AT2G45200 34.016 244 122 6 23 233 20 257 2.14e-34 124

Find 39 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGGATGCACGCTCTTTA+AGG 0.233741 2.1:-1077409 None:intergenic
GTCTTCCAGCGTTCGACTTT+TGG 0.265092 2.1:+1077791 MS.gene85954:CDS
ACCATTATACTTGCCCTTGT+TGG 0.314904 2.1:+1078176 MS.gene85954:CDS
TGATCGAACAAGATTAGATT+TGG 0.341869 2.1:+1077355 MS.gene85954:CDS
TGAGATCTTGATAAATTTCT+TGG 0.346054 2.1:-1076364 None:intergenic
CCATTATACTTGCCCTTGTT+GGG 0.360182 2.1:+1078177 MS.gene85954:CDS
CAATAAACATCATCTTTACT+TGG 0.366621 2.1:-1078237 None:intergenic
GTCCTCTGGTGGGTCTGAAA+TGG 0.402834 2.1:+1076319 MS.gene85954:CDS
CCCAACAAGGGCAAGTATAA+TGG 0.403543 2.1:-1078177 None:intergenic
CAGGCTTGGGTGTCCTCTGG+TGG 0.415447 2.1:+1076308 MS.gene85954:CDS
AGCTCAGTTGGATGAACAAA+TGG 0.421736 2.1:+1076166 MS.gene85954:CDS
ATGCAGGCTTGGGTGTCCTC+TGG 0.438772 2.1:+1076305 MS.gene85954:CDS
AACAAGATTAGATTTGGAAG+AGG 0.457292 2.1:+1077361 MS.gene85954:CDS
TCAACGAAAGCTTCTACAAA+AGG 0.463049 2.1:+1076209 MS.gene85954:CDS
CAGAGAGTGATCTGGAGTCT+TGG 0.464648 2.1:+1076240 MS.gene85954:CDS
TGAGGCAACAGAGAGTGATC+TGG 0.471531 2.1:+1076232 MS.gene85954:CDS
TAAACTCACAGATGCAGGCT+TGG 0.471681 2.1:+1076294 MS.gene85954:CDS
AAACTCACAGATGCAGGCTT+GGG 0.480195 2.1:+1076295 MS.gene85954:CDS
CTGTCCTGTACTGCGGCTGA+TGG 0.485234 2.1:-1077426 None:intergenic
ACAAGTAAACTCACAGATGC+AGG 0.514539 2.1:+1076289 MS.gene85954:CDS
AGGCTTGGGTGTCCTCTGGT+GGG 0.515681 2.1:+1076309 MS.gene85954:CDS
TCATAACTCATACAGTCATC+AGG 0.525906 2.1:-1075371 None:intergenic
GCATCCATCAGCCGCAGTAC+AGG 0.527799 2.1:+1077422 MS.gene85954:CDS
AAGAAAACTTGAAGCTCAGT+TGG 0.529386 2.1:+1076154 MS.gene85954:CDS
AGATTAGATTTGGAAGAGGG+TGG 0.530846 2.1:+1077365 MS.gene85954:CDS
CATCAGCCGCAGTACAGGAC+AGG 0.540958 2.1:+1077427 MS.gene85954:CDS
GCTTAGCTCTAAGATTAGAC+CGG 0.556599 2.1:-1077295 None:intergenic
ACAAGATTAGATTTGGAAGA+GGG 0.565048 2.1:+1077362 MS.gene85954:CDS
TCCCACCAAAAGTCGAACGC+TGG 0.611928 2.1:-1077796 None:intergenic
AAATGCATACGGACCCAACA+AGG 0.613983 2.1:-1078190 None:intergenic
CGTGAGCAGTCGCCTCCCAA+CGG 0.616247 2.1:+1077838 MS.gene85954:CDS
TGGATGCACGCTCTTTAAGG+AGG 0.630188 2.1:-1077406 None:intergenic
TCACAAGCACAAGCAACACT+CGG 0.631705 2.1:+1077761 MS.gene85954:CDS
GAAAGCTTCTACAAAAGGTG+AGG 0.666826 2.1:+1076214 MS.gene85954:CDS
AATGCATACGGACCCAACAA+GGG 0.667761 2.1:-1078189 None:intergenic
ACAGCAATCATACCGTTGGG+AGG 0.674625 2.1:-1077850 None:intergenic
AACCATTTCAGACCCACCAG+AGG 0.691979 2.1:-1076321 None:intergenic
TAACAACCTGTCCTGTACTG+CGG 0.715310 2.1:-1077433 None:intergenic
AATTTATCAAGATCTCACCC+AGG 0.754080 2.1:+1076370 MS.gene85954:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTAATGATAAATGAAA+AGG - chr2.1:1076566-1076585 None:intergenic 10.0%
!! GTTATAATAAATAATGTTAT+AGG - chr2.1:1077063-1077082 None:intergenic 10.0%
!! GTTTAAATAAATATTGAATA+AGG + chr2.1:1077877-1077896 MS.gene85954:intron 10.0%
!! TATAATAAATAATGTTATAG+GGG - chr2.1:1077061-1077080 None:intergenic 10.0%
!!! AAGCTTATATATAATTTTAT+AGG - chr2.1:1077557-1077576 None:intergenic 10.0%
!!! CAAAAATATTTGAAATATAA+AGG - chr2.1:1077208-1077227 None:intergenic 10.0%
!! GTAACAGTTATAATAAATAA+TGG - chr2.1:1077105-1077124 None:intergenic 15.0%
!!! TTTTTTTTTTCTTTCTTGAA+GGG + chr2.1:1076028-1076047 MS.gene85954:intron 15.0%
!!! TTTTTTTTTTTCTTTCTTGA+AGG + chr2.1:1076027-1076046 MS.gene85954:intron 15.0%
!! ACATTGGAATTGAAATAAAA+GGG - chr2.1:1076999-1077018 None:intergenic 20.0%
!! AGGTCAAGTATAAATTTATT+AGG - chr2.1:1076603-1076622 None:intergenic 20.0%
!! GAAAATAAGAAAAATGCATA+CGG - chr2.1:1078204-1078223 None:intergenic 20.0%
!! TTTAAATACATCTAACAAGT+TGG - chr2.1:1077255-1077274 None:intergenic 20.0%
!!! AAAAACTTGCTTTTGAAAAA+GGG - chr2.1:1075464-1075483 None:intergenic 20.0%
!!! AATGGTTTTATATCGTAAAT+TGG + chr2.1:1076184-1076203 MS.gene85954:CDS 20.0%
!!! ATCAAGTAATTTTGTTGAAT+TGG + chr2.1:1076002-1076021 MS.gene85954:intron 20.0%
!!! TAAGCAAAAAACTACTTTTA+AGG - chr2.1:1075627-1075646 None:intergenic 20.0%
!!! TCAAGTAATTTTGTTGAATT+GGG + chr2.1:1076003-1076022 MS.gene85954:intron 20.0%
!!! TCTTTTTATCTGTATTATGA+CGG - chr2.1:1075815-1075834 None:intergenic 20.0%
!!! TGCAATTTGTGTTTTAAAAA+AGG - chr2.1:1076731-1076750 None:intergenic 20.0%
!!! TTTTTCTTATTTTCATCTAC+TGG + chr2.1:1078210-1078229 MS.gene85954:CDS 20.0%
! AAAAGACGACTTAAAGTTTA+TGG + chr2.1:1075829-1075848 MS.gene85954:intron 25.0%
! AAACTTCAAATGAACATTCA+AGG - chr2.1:1077654-1077673 None:intergenic 25.0%
! AGATCAAATAATCAAGATGA+CGG + chr2.1:1075695-1075714 MS.gene85954:intron 25.0%
! CAAATGTCTGATATTAAAGA+TGG - chr2.1:1077609-1077628 None:intergenic 25.0%
! CATATTATATCATGTAGTTG+TGG + chr2.1:1076441-1076460 MS.gene85954:intron 25.0%
! GAAAAAATAATCCATTAGCT+TGG - chr2.1:1077632-1077651 None:intergenic 25.0%
! GACATTGGAATTGAAATAAA+AGG - chr2.1:1077000-1077019 None:intergenic 25.0%
! TAAAACTTGTGTAGTAGAAA+TGG - chr2.1:1078065-1078084 None:intergenic 25.0%
! TGAGATCTTGATAAATTTCT+TGG - chr2.1:1076367-1076386 None:intergenic 25.0%
! TTTCAATTCCAATGTCTTAT+AGG + chr2.1:1077004-1077023 MS.gene85954:intron 25.0%
!! AGGTTTAGTTTAAATAAGAG+TGG - chr2.1:1076873-1076892 None:intergenic 25.0%
!! GGTTTAGTTTAAATAAGAGT+GGG - chr2.1:1076872-1076891 None:intergenic 25.0%
!! GTTAGATGTATTTAAAATGC+AGG + chr2.1:1077259-1077278 MS.gene85954:intron 25.0%
!! GTTTAGTTTAAATAAGAGTG+GGG - chr2.1:1076871-1076890 None:intergenic 25.0%
!! TTTAGTTTAAATAAGAGTGG+GGG - chr2.1:1076870-1076889 None:intergenic 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG + chr2.1:1075637-1075656 MS.gene85954:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG - chr2.1:1075656-1075637 None:intergenic 25.0%
!! TTTTTGTAGAATCTCTATCA+CGG + chr2.1:1075479-1075498 MS.gene85954:intron 25.0%
!!! ACACAAGTTTTATTTCTCTA+TGG + chr2.1:1078073-1078092 MS.gene85954:intron 25.0%
!!! ATTATTTTAAATACTCCCTC+CGG - chr2.1:1075659-1075678 None:intergenic 25.0%
!!! CAAAAACTTGCTTTTGAAAA+AGG - chr2.1:1075465-1075484 None:intergenic 25.0%
!!! GCATAGTTCAAGTTAATTTT+AGG + chr2.1:1076053-1076072 MS.gene85954:intron 25.0%
!!! TAAGCAAAAAACTACTTTTG+AGG + chr2.1:1075538-1075557 MS.gene85954:intron 25.0%
!!! TTAAAATGCAGGAGTTTTAT+CGG + chr2.1:1077270-1077289 MS.gene85954:intron 25.0%
AACAAGATTAGATTTGGAAG+AGG + chr2.1:1077361-1077380 MS.gene85954:CDS 30.0%
AATAAAGCGAAAAAAGTCGA+TGG + chr2.1:1078151-1078170 MS.gene85954:CDS 30.0%
AATGACATCCTATAAGACAT+TGG - chr2.1:1077015-1077034 None:intergenic 30.0%
AATTGCCAGTTAGAACAATT+AGG - chr2.1:1076917-1076936 None:intergenic 30.0%
ACAAGATTAGATTTGGAAGA+GGG + chr2.1:1077362-1077381 MS.gene85954:CDS 30.0%
ACAGAAAAACCAAGTTATAC+AGG - chr2.1:1075935-1075954 None:intergenic 30.0%
ATAAAGAACCCTTTACATCA+GGG - chr2.1:1076508-1076527 None:intergenic 30.0%
ATATGCTCACACATAATGAT+TGG - chr2.1:1076426-1076445 None:intergenic 30.0%
CATTGAATGAATGATGTATG+TGG + chr2.1:1075563-1075582 MS.gene85954:intron 30.0%
CTATGGTGTTTGTTTAATGA+CGG + chr2.1:1078090-1078109 MS.gene85954:intron 30.0%
TAGCAAAAACCTGTATAACT+TGG + chr2.1:1075923-1075942 MS.gene85954:intron 30.0%
TATGCTCACACATAATGATT+GGG - chr2.1:1076425-1076444 None:intergenic 30.0%
TCAACTTCAGAATCAAAACA+GGG - chr2.1:1076766-1076785 None:intergenic 30.0%
TCTGATATTAAAGATGGCAT+TGG - chr2.1:1077603-1077622 None:intergenic 30.0%
TGATCGAACAAGATTAGATT+TGG + chr2.1:1077355-1077374 MS.gene85954:CDS 30.0%
TGGAATATTAGTTATTCTCC+TGG - chr2.1:1078045-1078064 None:intergenic 30.0%
TTGGAATTGAAATAAAAGGG+TGG - chr2.1:1076996-1077015 None:intergenic 30.0%
! ACTTTTTAATCTTCTCCATC+AGG + chr2.1:1077127-1077146 MS.gene85954:intron 30.0%
! TTGTCTTTTTAACCTTTTGC+AGG + chr2.1:1076130-1076149 MS.gene85954:intron 30.0%
! TTTTATCAAGATTGTTCCAC+TGG + chr2.1:1077490-1077509 MS.gene85954:intron 30.0%
!!! CTGAAGTTTTTTTTTTGTCC+AGG + chr2.1:1078024-1078043 MS.gene85954:intron 30.0%
!!! GAGAATTCTTTTGAAACTGA+AGG - chr2.1:1076623-1076642 None:intergenic 30.0%
AAGAAAACTTGAAGCTCAGT+TGG + chr2.1:1076154-1076173 MS.gene85954:CDS 35.0%
AATCATCTTGATCAGACTTC+TGG + chr2.1:1075750-1075769 MS.gene85954:intron 35.0%
AATTTATCAAGATCTCACCC+AGG + chr2.1:1076370-1076389 MS.gene85954:CDS 35.0%
AGTCACCTAATTGTTCTAAC+TGG + chr2.1:1076909-1076928 MS.gene85954:intron 35.0%
ATGCTCACACATAATGATTG+GGG - chr2.1:1076424-1076443 None:intergenic 35.0%
CATAAAGAACCCTTTACATC+AGG - chr2.1:1076509-1076528 None:intergenic 35.0%
CATTGAATGAGTGATGTATG+TGG - chr2.1:1075602-1075621 None:intergenic 35.0%
GTCAACTTCAGAATCAAAAC+AGG - chr2.1:1076767-1076786 None:intergenic 35.0%
GTTCTTTATGATAGCTGAGA+AGG + chr2.1:1076519-1076538 MS.gene85954:intron 35.0%
GTTTATTCTTGTACTGCAGA+TGG + chr2.1:1077727-1077746 MS.gene85954:intron 35.0%
TCAACGAAAGCTTCTACAAA+AGG + chr2.1:1076209-1076228 MS.gene85954:CDS 35.0%
TCATAACTCATACAGTCATC+AGG - chr2.1:1075374-1075393 None:intergenic 35.0%
! TATTAAAGATGGCATTGGAG+CGG - chr2.1:1077598-1077617 None:intergenic 35.0%
!! AGTTCTGAAAAACAGAGAAG+TGG - chr2.1:1076819-1076838 None:intergenic 35.0%
!! ATCTGTATTATGACGGACTA+CGG - chr2.1:1075808-1075827 None:intergenic 35.0%
AAATAAGAGTGGGGGTAGAA+GGG - chr2.1:1076862-1076881 None:intergenic 40.0%
AACTGTACTCCCTGATGTAA+AGG + chr2.1:1076496-1076515 MS.gene85954:intron 40.0%
ACAAGTAAACTCACAGATGC+AGG + chr2.1:1076289-1076308 MS.gene85954:CDS 40.0%
ACATCACAAAGCAACAGAGT+AGG - chr2.1:1077153-1077172 None:intergenic 40.0%
ACCATTATACTTGCCCTTGT+TGG + chr2.1:1078176-1078195 MS.gene85954:CDS 40.0%
ACTAAACCTTCTAACCTCTC+TGG + chr2.1:1076884-1076903 MS.gene85954:intron 40.0%
ACTGTACTCCCTGATGTAAA+GGG + chr2.1:1076497-1076516 MS.gene85954:intron 40.0%
ACTTCAGAATCAAAACAGGG+TGG - chr2.1:1076763-1076782 None:intergenic 40.0%
AGATTAGATTTGGAAGAGGG+TGG + chr2.1:1077365-1077384 MS.gene85954:CDS 40.0%
AGCTCAGTTGGATGAACAAA+TGG + chr2.1:1076166-1076185 MS.gene85954:CDS 40.0%
AGCTCATACGATGTAATACC+TGG - chr2.1:1076391-1076410 None:intergenic 40.0%
CCATTATACTTGCCCTTGTT+GGG + chr2.1:1078177-1078196 MS.gene85954:CDS 40.0%
GAAAGCTTCTACAAAAGGTG+AGG + chr2.1:1076214-1076233 MS.gene85954:CDS 40.0%
GCTCATACGATGTAATACCT+GGG - chr2.1:1076390-1076409 None:intergenic 40.0%
TAAATAAGAGTGGGGGTAGA+AGG - chr2.1:1076863-1076882 None:intergenic 40.0%
TCAACAGCAATCATACCGTT+GGG - chr2.1:1077856-1077875 None:intergenic 40.0%
TCAGACATTTGCCAAGCTAA+TGG + chr2.1:1077618-1077637 MS.gene85954:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG + chr2.1:1075641-1075660 MS.gene85954:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr2.1:1075660-1075641 None:intergenic 40.0%
TGTCTGACAGATCTAAGCTT+AGG - chr2.1:1077232-1077251 None:intergenic 40.0%
TTCAACAGCAATCATACCGT+TGG - chr2.1:1077857-1077876 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr2.1:1075640-1075659 MS.gene85954:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr2.1:1075659-1075640 None:intergenic 40.0%
! AATCAAGATGACGGCAGTAT+TGG + chr2.1:1075704-1075723 MS.gene85954:intron 40.0%
! ACGGCTTTTAACAATGTTGC+AGG + chr2.1:1078109-1078128 MS.gene85954:intron 40.0%
! AGGGAGTACAGTTTGCAAAT+AGG - chr2.1:1076489-1076508 None:intergenic 40.0%
! ATGAGAGAGGAGTATAACAG+AGG + chr2.1:1076653-1076672 MS.gene85954:intron 40.0%
! CAAGTTTTCTTGCCTGCAAA+AGG - chr2.1:1076145-1076164 None:intergenic 40.0%
! CTCTTTTCTACAGATGAGAG+AGG + chr2.1:1076640-1076659 MS.gene85954:intron 40.0%
! TGAGAGAGGAGTATAACAGA+GGG + chr2.1:1076654-1076673 MS.gene85954:intron 40.0%
!! CTCTGTTGCTTTGTGATGTT+AGG + chr2.1:1077154-1077173 MS.gene85954:intron 40.0%
!! GCTTAGCTCTAAGATTAGAC+CGG - chr2.1:1077298-1077317 None:intergenic 40.0%
!! GGAGTCTTTCTGAAGCTAAA+AGG - chr2.1:1076710-1076729 None:intergenic 40.0%
AAACTCACAGATGCAGGCTT+GGG + chr2.1:1076295-1076314 MS.gene85954:CDS 45.0%
AAATGCATACGGACCCAACA+AGG - chr2.1:1078193-1078212 None:intergenic 45.0%
AATGCATACGGACCCAACAA+GGG - chr2.1:1078192-1078211 None:intergenic 45.0%
AGAAGTGGATCAACTGCAAC+GGG - chr2.1:1076804-1076823 None:intergenic 45.0%
AGAATCTCTATCACGGCTTC+TGG + chr2.1:1075486-1075505 MS.gene85954:intron 45.0%
CCCAACAAGGGCAAGTATAA+TGG - chr2.1:1078180-1078199 None:intergenic 45.0%
GAGAAGTGGATCAACTGCAA+CGG - chr2.1:1076805-1076824 None:intergenic 45.0%
TAAACTCACAGATGCAGGCT+TGG + chr2.1:1076294-1076313 MS.gene85954:CDS 45.0%
TAACAACCTGTCCTGTACTG+CGG - chr2.1:1077436-1077455 None:intergenic 45.0%
TAATAAGTGACGCACTCCAG+TGG - chr2.1:1077509-1077528 None:intergenic 45.0%
TCAAAACAGGGTGGACGAAT+AGG - chr2.1:1076754-1076773 None:intergenic 45.0%
TCACAAGCACAAGCAACACT+CGG + chr2.1:1077761-1077780 MS.gene85954:CDS 45.0%
TGGCTAATACTTACTCCCTC+CGG + chr2.1:1075506-1075525 MS.gene85954:intron 45.0%
! AATTAGGTGACTAGCCAGAG+AGG - chr2.1:1076901-1076920 None:intergenic 45.0%
! GAGAGAGGAGTATAACAGAG+GGG + chr2.1:1076655-1076674 MS.gene85954:intron 45.0%
! TGATGGATGCACGCTCTTTA+AGG - chr2.1:1077412-1077431 None:intergenic 45.0%
!! TTATAATAAATAATGTTATA+GGG - chr2.1:1077062-1077081 None:intergenic 5.0%
!! TTATTATTTACTTATATATT+TGG + chr2.1:1077523-1077542 MS.gene85954:intron 5.0%
!!! AATATATTTTTTTGTTTTTT+AGG + chr2.1:1076086-1076105 MS.gene85954:intron 5.0%
AAACAGAACAACCCCAGCTG+TGG - chr2.1:1077696-1077715 None:intergenic 50.0%
AACCATTTCAGACCCACCAG+AGG - chr2.1:1076324-1076343 None:intergenic 50.0%
AAGCAACAGAGTAGGCCTGA+TGG - chr2.1:1077145-1077164 None:intergenic 50.0%
ACAGATGTCTGATCCACAGC+TGG + chr2.1:1077680-1077699 MS.gene85954:intron 50.0%
ACAGCAATCATACCGTTGGG+AGG - chr2.1:1077853-1077872 None:intergenic 50.0%
AGATGTCTGATCCACAGCTG+GGG + chr2.1:1077682-1077701 MS.gene85954:intron 50.0%
CAGATGTCTGATCCACAGCT+GGG + chr2.1:1077681-1077700 MS.gene85954:intron 50.0%
TGAGGCAACAGAGAGTGATC+TGG + chr2.1:1076232-1076251 MS.gene85954:CDS 50.0%
! CAGAGAGTGATCTGGAGTCT+TGG + chr2.1:1076240-1076259 MS.gene85954:CDS 50.0%
! GACTAGCCAGAGAGGTTAGA+AGG - chr2.1:1076893-1076912 None:intergenic 50.0%
! GTCTTCCAGCGTTCGACTTT+TGG + chr2.1:1077791-1077810 MS.gene85954:CDS 50.0%
! TCCAGCGTTCGACTTTTGGT+GGG + chr2.1:1077795-1077814 MS.gene85954:CDS 50.0%
! TGGATGCACGCTCTTTAAGG+AGG - chr2.1:1077409-1077428 None:intergenic 50.0%
! TTCCAGCGTTCGACTTTTGG+TGG + chr2.1:1077794-1077813 MS.gene85954:CDS 50.0%
!!! TGCAGGAGTTTTATCGGCTC+CGG + chr2.1:1077276-1077295 MS.gene85954:intron 50.0%
CAACTGCAACGGGAGATCGA+CGG - chr2.1:1076794-1076813 None:intergenic 55.0%
TCCCACCAAAAGTCGAACGC+TGG - chr2.1:1077799-1077818 None:intergenic 55.0%
! GTCCTCTGGTGGGTCTGAAA+TGG + chr2.1:1076319-1076338 MS.gene85954:CDS 55.0%
AGGCTTGGGTGTCCTCTGGT+GGG + chr2.1:1076309-1076328 MS.gene85954:CDS 60.0%
ATGCAGGCTTGGGTGTCCTC+TGG + chr2.1:1076305-1076324 MS.gene85954:CDS 60.0%
CATCAGCCGCAGTACAGGAC+AGG + chr2.1:1077427-1077446 MS.gene85954:CDS 60.0%
CTGTCCTGTACTGCGGCTGA+TGG - chr2.1:1077429-1077448 None:intergenic 60.0%
GCATCCATCAGCCGCAGTAC+AGG + chr2.1:1077422-1077441 MS.gene85954:CDS 60.0%
CAGGCTTGGGTGTCCTCTGG+TGG + chr2.1:1076308-1076327 MS.gene85954:CDS 65.0%
CGTGAGCAGTCGCCTCCCAA+CGG + chr2.1:1077838-1077857 MS.gene85954:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 1075315 1078244 1075315 ID=MS.gene85954
chr2.1 mRNA 1075315 1078244 1075315 ID=MS.gene85954.t1;Parent=MS.gene85954
chr2.1 exon 1075315 1075380 1075315 ID=MS.gene85954.t1.exon1;Parent=MS.gene85954.t1
chr2.1 CDS 1075315 1075380 1075315 ID=cds.MS.gene85954.t1;Parent=MS.gene85954.t1
chr2.1 exon 1076152 1076391 1076152 ID=MS.gene85954.t1.exon2;Parent=MS.gene85954.t1
chr2.1 CDS 1076152 1076391 1076152 ID=cds.MS.gene85954.t1;Parent=MS.gene85954.t1
chr2.1 exon 1077281 1077448 1077281 ID=MS.gene85954.t1.exon3;Parent=MS.gene85954.t1
chr2.1 CDS 1077281 1077448 1077281 ID=cds.MS.gene85954.t1;Parent=MS.gene85954.t1
chr2.1 exon 1077746 1077859 1077746 ID=MS.gene85954.t1.exon4;Parent=MS.gene85954.t1
chr2.1 CDS 1077746 1077859 1077746 ID=cds.MS.gene85954.t1;Parent=MS.gene85954.t1
chr2.1 exon 1078131 1078244 1078131 ID=MS.gene85954.t1.exon5;Parent=MS.gene85954.t1
chr2.1 CDS 1078131 1078244 1078131 ID=cds.MS.gene85954.t1;Parent=MS.gene85954.t1
Gene Sequence

>MS.gene85954

ATGAAATCTAATGATCACGTTGATGTTCTATTTCTCGCATTTATTCTTGTTATGTACCTGATGACTGTATGAGTTATGACTATACTAGATTAATATACACTTCACTGCATATCAAGATATATTTTGTTAACAACTCGCTGTGTTTTCCCTTTTTCAAAAGCAAGTTTTTGTAGAATCTCTATCACGGCTTCTGGCTAATACTTACTCCCTCCGGTCACTATTATAAGCAAAAAACTACTTTTGAGGTTCATTGAATGAATGATGTATGTGGTCTATATTAATGACCACATACATCACTCATTCAATGAACCTTAAAAGTAGTTTTTTGCTTATAATAGTGACCGGAGGGAGTATTTAAAATAATTTGCAATAAGCTTAAAAGATCAAATAATCAAGATGACGGCAGTATTGGTTAATATTGTCATTTTATGCACTAATCATCTTGATCAGACTTCTGGATATGATAGCTAAATCATTTGAAATCATATAACCGTAGTCCGTCATAATACAGATAAAAAGACGACTTAAAGTTTATGGAATTTCAGTCATACTAGTTGTTCTGTTTGTAATTATAATACTTAAACATGTATTCAATCACTTTTTTGTTGTAGCAAAAACCTGTATAACTTGGTTTTTCTGTGCTATTCATGATTAGTCATTAACTCATTATAGTACTAATAACATTATATCAAGTAATTTTGTTGAATTGGGTTTTTTTTTTTTCTTTCTTGAAGGGATGCATAGTTCAAGTTAATTTTAGGTTTACTATTTAATATATTTTTTTGTTTTTTAGGCAAATTATTAACAGAAATTTGTTGTCTTTTTAACCTTTTGCAGGCAAGAAAACTTGAAGCTCAGTTGGATGAACAAATGGTTTTATATCGTAAATTGGTTTCAACGAAAGCTTCTACAAAAGGTGAGGCAACAGAGAGTGATCTGGAGTCTTGGATTGAACGTTTACTTAATCAACTCAAACAAGTAAACTCACAGATGCAGGCTTGGGTGTCCTCTGGTGGGTCTGAAATGGTTTCTCATACACTGACTAGACACCAAGAAATTTATCAAGATCTCACCCAGGTATTACATCGTATGAGCTTGAGCTACTACCCCAATCATTATGTGTGAGCATATTATATCATGTAGTTGTGGCTTTATTGCATTTTGTGTAAAACCTATTTGCAAACTGTACTCCCTGATGTAAAGGGTTCTTTATGATAGCTGAGAAGGATTTGAATTTAATATATTAAGCCTTTTCATTTATCATTAAATATCATAACAAGTTTTGCCTAATAAATTTATACTTGACCTTCAGTTTCAAAAGAATTCTCTTTTCTACAGATGAGAGAGGAGTATAACAGAGGGGTAAATTTTATTGTTATCATGTAAAACTTTCCTTTTAGCTTCAGAAAGACTCCTTTTTTAAAACACAAATTGCACCTATTCGTCCACCCTGTTTTGATTCTGAAGTTGACATCTCCGTCGATCTCCCGTTGCAGTTGATCCACTTCTCTGTTTTTCAGAACTAACAAATCTGAATCTCATCTCCCTTCTACCCCCACTCTTATTTAAACTAAACCTTCTAACCTCTCTGGCTAGTCACCTAATTGTTCTAACTGGCAATTGATACTTGACTGTAACTGACTTGTAACAAACTATTGATGCAATTTGCAAATCTCTACCACCCTTTTATTTCAATTCCAATGTCTTATAGGATGTCATTGCTAGTTGAAATAGAAGTTCTAACCCCTATAACATTATTTATTATAACTTTTTTTTTTTTTGAACAGCCATTATTTATTATAACTGTTACATACTTTTTAATCTTCTCCATCAGGCCTACTCTGTTGCTTTGTGATGTTAGGCTTGTTTATATTGATTGATTAGTTATTTCCTTTATATTTCAAATATTTTTGTCCTAAGCTTAGATCTGTCAGACACCAACTTGTTAGATGTATTTAAAATGCAGGAGTTTTATCGGCTCCGGTCTAATCTTAGAGCTAAGCAAGAACACGCTTCACTACTTGATGATTTTAAAGAGCTTGATCGAACAAGATTAGATTTGGAAGAGGGTGGAAGTTCTGAACAGCAAAACCTCCTTAAAGAGCGTGCATCCATCAGCCGCAGTACAGGACAGGTTGTTAACATGAAACAATTTTTTGTTATAAAGTTCTGTTCTTTTATCAAGATTGTTCCACTGGAGTGCGTCACTTATTATTTACTTATATATTTGGATACTCTACCTATAAAATTATATATAAGCTTTTAATTATTTGATCTATGCCGCTCCAATGCCATCTTTAATATCAGACATTTGCCAAGCTAATGGATTATTTTTTCCTTGAATGTTCATTTGAAGTTTAAATATACAGATGTCTGATCCACAGCTGGGGTTGTTCTGTTTTAATATACTGTGTTTATTCTTGTACTGCAGATGGACACTGTTATTTCACAAGCACAAGCAACACTCGGTGCACTAGTCTTCCAGCGTTCGACTTTTGGTGGGATCAATTCAAAGCTCAGCAACGTGAGCAGTCGCCTCCCAACGGTATGATTGCTGTTGAAGTTTAAATAAATATTGAATAAGGATGAAGATTAGTTTTAAATAAATATTGAATAAAGATGAAGATTAGTTTCTGTGAAATATGATAATAAAATTCATCTTTCATGAGAATGAATAAATTGTTCAAAATATTGCAGTCTTATTTGATTCTGAAGTTTTTTTTTTGTCCAGGAGAATAACTAATATTCCATTTCTACTACACAAGTTTTATTTCTCTATGGTGTTTGTTTAATGACGGCTTTTAACAATGTTGCAGGTAAATACTATTCTGTCAGCAATAAAGCGAAAAAAGTCGATGGATACCATTATACTTGCCCTTGTTGGGTCCGTATGCATTTTTCTTATTTTCATCTACTGGCTAACCAAGTAA
Protein sequence

>MS.gene85954.t1

MKSNDHVDVLFLAFILVMYLMTARKLEAQLDEQMVLYRKLVSTKASTKGEATESDLESWIERLLNQLKQVNSQMQAWVSSGGSEMVSHTLTRHQEIYQDLTQEFYRLRSNLRAKQEHASLLDDFKELDRTRLDLEEGGSSEQQNLLKERASISRSTGQMDTVISQAQATLGALVFQRSTFGGINSKLSNVSSRLPTVNTILSAIKRKKSMDTIILALVGSVCIFLIFIYWLTK