Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48916.t1 | XP_013455082.1 | 99.2 | 240 | 2 | 0 | 1 | 240 | 1 | 240 | 3.60E-125 | 457.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48916.t1 | O22151 | 81.1 | 254 | 30 | 1 | 5 | 240 | 4 | 257 | 2.0e-108 | 393.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48916.t1 | A0A072UTB1 | 99.2 | 240 | 2 | 0 | 1 | 240 | 1 | 240 | 2.6e-125 | 457.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene40807 | MS.gene48916 | PPI |
| MS.gene48916 | MS.gene056096 | PPI |
| MS.gene051072 | MS.gene48916 | PPI |
| MS.gene86991 | MS.gene48916 | PPI |
| MS.gene056094 | MS.gene48916 | PPI |
| MS.gene067465 | MS.gene48916 | PPI |
| MS.gene48916 | MS.gene39450 | PPI |
| MS.gene052477 | MS.gene48916 | PPI |
| MS.gene005292 | MS.gene48916 | PPI |
| MS.gene83589 | MS.gene48916 | PPI |
| MS.gene44169 | MS.gene48916 | PPI |
| MS.gene48916 | MS.gene85954 | PPI |
| MS.gene33790 | MS.gene48916 | PPI |
| MS.gene44168 | MS.gene48916 | PPI |
| MS.gene072972 | MS.gene48916 | PPI |
| MS.gene31936 | MS.gene48916 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48916.t1 | MTR_4g025850 | 99.167 | 240 | 2 | 0 | 1 | 240 | 1 | 240 | 2.06e-176 | 484 |
| MS.gene48916.t1 | MTR_4g025850 | 94.253 | 174 | 10 | 0 | 1 | 174 | 1 | 174 | 4.10e-119 | 337 |
| MS.gene48916.t1 | MTR_4g006570 | 35.833 | 240 | 133 | 5 | 7 | 240 | 1 | 225 | 2.38e-45 | 151 |
| MS.gene48916.t1 | MTR_2g104910 | 36.481 | 233 | 127 | 6 | 14 | 240 | 6 | 223 | 2.96e-41 | 140 |
| MS.gene48916.t1 | MTR_4g006570 | 36.123 | 227 | 124 | 5 | 20 | 240 | 30 | 241 | 2.08e-40 | 139 |
| MS.gene48916.t1 | MTR_4g006570 | 36.123 | 227 | 124 | 5 | 20 | 240 | 39 | 250 | 3.13e-40 | 139 |
| MS.gene48916.t1 | MTR_4g006570 | 36.123 | 227 | 124 | 5 | 20 | 240 | 44 | 255 | 3.30e-40 | 139 |
| MS.gene48916.t1 | MTR_2g104910 | 36.283 | 226 | 123 | 6 | 21 | 240 | 2 | 212 | 5.27e-38 | 132 |
| MS.gene48916.t1 | MTR_4g006570 | 38.798 | 183 | 101 | 3 | 64 | 240 | 23 | 200 | 2.41e-37 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48916.t1 | AT2G45200 | 87.288 | 236 | 30 | 0 | 5 | 240 | 4 | 239 | 4.53e-154 | 427 |
| MS.gene48916.t1 | AT2G45200 | 81.102 | 254 | 30 | 1 | 5 | 240 | 4 | 257 | 1.48e-149 | 417 |
| MS.gene48916.t1 | AT1G15880 | 36.087 | 230 | 135 | 4 | 12 | 240 | 5 | 223 | 3.77e-41 | 140 |
Find 66 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGTTTCTCATCATTTAT+TGG | 0.113073 | 4.3:-72677977 | MS.gene48916:CDS |
| GGATGATATTTCGGACTTTA+AGG | 0.169196 | 4.3:-72679350 | MS.gene48916:intron |
| CAATAACTGCTGACAGAATA+AGG | 0.238804 | 4.3:+72678009 | None:intergenic |
| CAAGGAAAAGTGAGGCTTCT+TGG | 0.277079 | 4.3:-72678194 | MS.gene48916:CDS |
| ACCGAGCAAGCTACGAATAA+TGG | 0.289236 | 4.3:+72678160 | None:intergenic |
| TTCTGTTAGGGATGATATTT+CGG | 0.292259 | 4.3:-72679359 | MS.gene48916:CDS |
| AAGGAAAAGTGAGGCTTCTT+GGG | 0.331136 | 4.3:-72678193 | MS.gene48916:CDS |
| CCGAGCAAGCTACGAATAAT+GGG | 0.354967 | 4.3:+72678161 | None:intergenic |
| GTTGTTTGCTTCAGGAGTTC+AGG | 0.357787 | 4.3:-72679432 | MS.gene48916:intron |
| AATCTGGATTTGCAGGAATC+AGG | 0.363110 | 4.3:-72696085 | MS.gene48916:CDS |
| ATCCTTGCTCGAGAAACTAT+TGG | 0.377748 | 4.3:-72693759 | MS.gene48916:CDS |
| CTGTTGGGTCCAGTAGGTCT+TGG | 0.402163 | 4.3:-72693805 | MS.gene48916:CDS |
| TACTACGAATCTGGATTTGC+AGG | 0.403838 | 4.3:-72696092 | MS.gene48916:CDS |
| CCTCTTGTTATTAGGTTATG+TGG | 0.405067 | 4.3:-72693843 | MS.gene48916:intron |
| TCAAGCTACAAGAGCTGTCT+TGG | 0.408027 | 4.3:-72678246 | MS.gene48916:CDS |
| AACTTCTTCTAGCAGGCATC+TGG | 0.415831 | 4.3:-72679265 | MS.gene48916:intron |
| GGTTCTCAGAGGGCATTGTT+TGG | 0.419536 | 4.3:-72678224 | MS.gene48916:CDS |
| AATGAGGGATACTACGAATC+TGG | 0.425976 | 4.3:-72696101 | MS.gene48916:CDS |
| CATGGACTTCCAAGACCTAC+TGG | 0.433265 | 4.3:+72693796 | None:intergenic |
| GTCTTGGAAGTCCATGGAAA+TGG | 0.434001 | 4.3:-72693789 | MS.gene48916:CDS |
| CATTGAGTTTATGTTCCCCT+TGG | 0.435788 | 4.3:+72679405 | None:intergenic |
| AGTAGATGTGCTGCATCTGC+TGG | 0.459514 | 4.3:-72693719 | MS.gene48916:CDS |
| CCCATTATTCGTAGCTTGCT+CGG | 0.465386 | 4.3:-72678161 | MS.gene48916:intron |
| TACTTCTGTAACACAGAAGT+TGG | 0.472524 | 4.3:-72693690 | MS.gene48916:CDS |
| ATAGTTTCTCGAGCAAGGAT+TGG | 0.479613 | 4.3:+72693762 | None:intergenic |
| GATGGTGGCTCACCATCTGT+TGG | 0.482816 | 4.3:-72693821 | MS.gene48916:CDS |
| GGATTTGCAGGAATCAGGTT+GGG | 0.485367 | 4.3:-72696080 | MS.gene48916:CDS |
| GGATTGGATTTCCATTTCCA+TGG | 0.485603 | 4.3:+72693778 | None:intergenic |
| CGATAAGAAGGCGAAAATCA+AGG | 0.488091 | 4.3:-72678037 | MS.gene48916:CDS |
| AATAACTGCTGACAGAATAA+GGG | 0.496188 | 4.3:+72678010 | None:intergenic |
| TCACGTAATAACTGCATCCT+TGG | 0.499294 | 4.3:+72679232 | None:intergenic |
| GTCCAATAGTTTCTCGAGCA+AGG | 0.499653 | 4.3:+72693757 | None:intergenic |
| CAAGCTACAAGAGCTGTCTT+GGG | 0.501409 | 4.3:-72678245 | MS.gene48916:CDS |
| CGTGAGAGAGCGTCCATCCA+TGG | 0.507623 | 4.3:-72679214 | MS.gene48916:CDS |
| GCATTGTTTGGCGATGTTCA+AGG | 0.518710 | 4.3:-72678212 | MS.gene48916:CDS |
| CTTTCTTCTTACGCTAAACT+CGG | 0.523733 | 4.3:-72696007 | MS.gene48916:CDS |
| TTGTTATTAGGTTATGTGGA+TGG | 0.526319 | 4.3:-72693839 | MS.gene48916:intron |
| ATGGTGGCTCACCATCTGTT+GGG | 0.536398 | 4.3:-72693820 | MS.gene48916:CDS |
| TTCTGTGTTACAGAAGTAGC+TGG | 0.537855 | 4.3:+72693695 | None:intergenic |
| CAGTAGGTCTTGGAAGTCCA+TGG | 0.539641 | 4.3:-72693795 | MS.gene48916:CDS |
| AGCTGTCTTGGGTTCTCAGA+GGG | 0.552364 | 4.3:-72678234 | MS.gene48916:CDS |
| CACCATCTGTTGGGTCCAGT+AGG | 0.560246 | 4.3:-72693811 | MS.gene48916:CDS |
| TGGATTTGCAGGAATCAGGT+TGG | 0.560896 | 4.3:-72696081 | MS.gene48916:CDS |
| GATAAGAAGGCGAAAATCAA+GGG | 0.563741 | 4.3:-72678036 | MS.gene48916:CDS |
| GGGGAACATAAACTCAATGA+AGG | 0.567757 | 4.3:-72679401 | MS.gene48916:CDS |
| TTACATGAGACGTACTTCCA+TGG | 0.570002 | 4.3:+72679197 | None:intergenic |
| CTGTAACACAGAAGTTGGCT+AGG | 0.573524 | 4.3:-72693685 | MS.gene48916:CDS |
| AGGTTGGGAGGAACTTCGTA+AGG | 0.582246 | 4.3:-72696065 | MS.gene48916:CDS |
| CAGGAGTTCAGGCGTACCAA+GGG | 0.582622 | 4.3:-72679421 | MS.gene48916:CDS |
| TCAGGAGTTCAGGCGTACCA+AGG | 0.584470 | 4.3:-72679422 | MS.gene48916:intron |
| TTATTAGGTTATGTGGATGG+TGG | 0.585061 | 4.3:-72693836 | MS.gene48916:intron |
| ATGAGACGTACTTCCATGGA+TGG | 0.587703 | 4.3:+72679201 | None:intergenic |
| AGGAAAAGTGAGGCTTCTTG+GGG | 0.588008 | 4.3:-72678192 | MS.gene48916:CDS |
| CTCGGTGCTAGATTCACTCA+AGG | 0.599442 | 4.3:-72695989 | MS.gene48916:CDS |
| AGATGCAGCACATCTACTCA+TGG | 0.603446 | 4.3:+72693724 | None:intergenic |
| AAGGAAGCGCGTAAGATCGA+AGG | 0.606205 | 4.3:-72696046 | MS.gene48916:CDS |
| GACCTACTGGACCCAACAGA+TGG | 0.606510 | 4.3:+72693809 | None:intergenic |
| TATTCTTCATGAGTTTACCC+AGG | 0.621068 | 4.3:-72693654 | MS.gene48916:intron |
| TTTGCAGGAATCAGGTTGGG+AGG | 0.625607 | 4.3:-72696077 | MS.gene48916:CDS |
| CCAATAAATGATGAGAAACA+AGG | 0.636384 | 4.3:+72677977 | None:intergenic |
| GAGCTGTCTTGGGTTCTCAG+AGG | 0.646743 | 4.3:-72678235 | MS.gene48916:CDS |
| GCGATGTTCAAGGAAAAGTG+AGG | 0.672498 | 4.3:-72678202 | MS.gene48916:CDS |
| AGGAGTTCAGGCGTACCAAG+GGG | 0.707609 | 4.3:-72679420 | MS.gene48916:CDS |
| CGAGCAAGCTACGAATAATG+GGG | 0.708413 | 4.3:+72678162 | None:intergenic |
| GGTGCTAGATTCACTCAAGG+AGG | 0.719729 | 4.3:-72695986 | MS.gene48916:intron |
| CATCTGGTAGTATGTCACCA+AGG | 0.721954 | 4.3:-72679249 | MS.gene48916:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 72677965 | 72696122 | 72677965 | ID=MS.gene48916 |
| chr4.3 | mRNA | 72677965 | 72696122 | 72677965 | ID=MS.gene48916.t1;Parent=MS.gene48916 |
| chr4.3 | exon | 72695987 | 72696122 | 72695987 | ID=MS.gene48916.t1.exon1;Parent=MS.gene48916.t1 |
| chr4.3 | CDS | 72695987 | 72696122 | 72695987 | ID=cds.MS.gene48916.t1;Parent=MS.gene48916.t1 |
| chr4.3 | exon | 72693655 | 72693851 | 72693655 | ID=MS.gene48916.t1.exon2;Parent=MS.gene48916.t1 |
| chr4.3 | CDS | 72693655 | 72693851 | 72693655 | ID=cds.MS.gene48916.t1;Parent=MS.gene48916.t1 |
| chr4.3 | exon | 72679351 | 72679440 | 72679351 | ID=MS.gene48916.t1.exon3;Parent=MS.gene48916.t1 |
| chr4.3 | CDS | 72679351 | 72679440 | 72679351 | ID=cds.MS.gene48916.t1;Parent=MS.gene48916.t1 |
| chr4.3 | exon | 72679201 | 72679272 | 72679201 | ID=MS.gene48916.t1.exon4;Parent=MS.gene48916.t1 |
| chr4.3 | CDS | 72679201 | 72679272 | 72679201 | ID=cds.MS.gene48916.t1;Parent=MS.gene48916.t1 |
| chr4.3 | exon | 72678162 | 72678291 | 72678162 | ID=MS.gene48916.t1.exon5;Parent=MS.gene48916.t1 |
| chr4.3 | CDS | 72678162 | 72678291 | 72678162 | ID=cds.MS.gene48916.t1;Parent=MS.gene48916.t1 |
| chr4.3 | exon | 72677965 | 72678062 | 72677965 | ID=MS.gene48916.t1.exon6;Parent=MS.gene48916.t1 |
| chr4.3 | CDS | 72677965 | 72678062 | 72677965 | ID=cds.MS.gene48916.t1;Parent=MS.gene48916.t1 |
| Gene Sequence |
| Protein sequence |
