AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86112


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86112.t1 MTR_6g014270 91.329 173 15 0 1 173 1 173 3.34e-115 335
MS.gene86112.t1 MTR_6g014195 81.977 172 29 1 2 173 5 174 1.61e-100 288
MS.gene86112.t1 MTR_6g014186 79.191 173 34 1 1 173 1 171 9.79e-97 287
MS.gene86112.t1 MTR_6g014150 80.347 173 32 1 1 173 1 171 2.07e-90 272
MS.gene86112.t1 MTR_6g014200 79.769 173 33 1 1 173 1 171 6.81e-89 268
MS.gene86112.t1 MTR_0288s0020 69.006 171 52 1 3 173 6 175 6.78e-82 249
MS.gene86112.t1 MTR_6g013440 67.442 172 54 1 2 173 6 175 1.83e-80 246
MS.gene86112.t1 MTR_6g014300 69.186 172 51 1 2 173 7 176 2.43e-80 246
MS.gene86112.t1 MTR_6g014040 66.272 169 56 1 5 173 8 175 6.29e-79 243
MS.gene86112.t1 MTR_6g014020 65.680 169 56 1 5 173 8 174 3.56e-77 238
MS.gene86112.t1 MTR_3g008010 64.327 171 56 2 3 173 6 171 9.98e-75 232
MS.gene86112.t1 MTR_6g014290 62.573 171 59 2 3 173 6 171 3.82e-73 228
MS.gene86112.t1 MTR_7g011470 66.474 173 56 1 3 175 6 176 4.01e-73 227
MS.gene86112.t1 MTR_8g059425 63.743 171 58 2 3 173 6 172 4.38e-73 228
MS.gene86112.t1 MTR_6g013450 63.905 169 59 1 5 173 8 174 6.39e-73 227
MS.gene86112.t1 MTR_6g014310 68.639 169 51 1 5 173 9 175 7.92e-73 227
MS.gene86112.t1 MTR_6g014240 66.474 173 42 2 1 173 1 157 8.95e-73 226
MS.gene86112.t1 MTR_7g011470 66.667 171 55 1 3 173 6 174 6.24e-72 224
MS.gene86112.t1 MTR_6g011340 66.272 169 55 1 5 173 8 174 1.50e-71 223
MS.gene86112.t1 MTR_6g013990 66.667 171 55 1 3 173 6 174 4.81e-71 222
MS.gene86112.t1 MTR_6g013960 61.404 171 64 1 3 173 6 174 7.60e-71 222
MS.gene86112.t1 MTR_6g014170 64.912 171 55 2 3 173 6 171 8.49e-71 221
MS.gene86112.t1 MTR_6g014220 64.912 171 55 2 3 173 6 171 5.42e-70 219
MS.gene86112.t1 MTR_6g014187 62.573 171 62 1 3 173 6 174 6.81e-70 219
MS.gene86112.t1 MTR_6g014250 60.234 171 63 2 3 173 6 171 2.35e-69 218
MS.gene86112.t1 MTR_0199s0010 59.064 171 64 3 3 173 6 170 1.15e-68 210
MS.gene86112.t1 MTR_8g007585 59.064 171 64 3 3 173 6 170 9.93e-68 211
MS.gene86112.t1 MTR_0475s0020 76.000 125 29 1 3 127 6 129 1.39e-65 199
MS.gene86112.t1 MTR_6g014050 68.047 169 52 1 5 173 9 175 2.17e-65 207
MS.gene86112.t1 MTR_6g013920 61.765 170 62 1 4 173 7 173 3.59e-61 197
MS.gene86112.t1 MTR_5g058320 58.580 169 52 2 5 173 8 158 2.26e-60 194
MS.gene86112.t1 MTR_6g013900 58.824 170 67 1 4 173 7 173 9.55e-59 190
MS.gene86112.t1 MTR_5g464760 52.907 172 73 3 3 173 8 172 4.35e-57 186
MS.gene86112.t1 MTR_5g464810 52.976 168 71 3 7 173 12 172 7.45e-56 183
MS.gene86112.t1 MTR_5g064240 52.976 168 71 3 7 173 12 172 7.45e-56 183
MS.gene86112.t1 MTR_2g008210 52.941 170 70 2 4 173 8 167 6.63e-55 180
MS.gene86112.t1 MTR_0036s0220 50.888 169 75 3 6 173 11 172 7.08e-54 177
MS.gene86112.t1 MTR_3g479620 48.555 173 82 2 3 175 2 167 1.47e-53 168
MS.gene86112.t1 MTR_3g479520 47.368 171 83 2 4 174 12 175 1.09e-48 164
MS.gene86112.t1 MTR_3g479490 48.538 171 81 2 3 173 2 165 1.20e-47 161
MS.gene86112.t1 MTR_6g013680 61.475 122 45 1 52 173 2 121 1.82e-47 159
MS.gene86112.t1 MTR_3g079480 46.471 170 84 2 4 173 18 180 4.21e-46 157
MS.gene86112.t1 MTR_4g094220 41.618 173 96 2 1 173 1 168 5.95e-38 135
MS.gene86112.t1 MTR_3g479470 41.765 170 92 2 4 173 10 172 1.12e-37 135
MS.gene86112.t1 MTR_6g014160 45.029 171 74 3 3 173 6 156 1.14e-37 133
MS.gene86112.t1 MTR_8g043470 57.265 117 30 2 56 172 9 105 5.10e-36 129
MS.gene86112.t1 MTR_7g013260 28.994 169 106 4 6 174 5 159 5.08e-18 80.9
MS.gene86112.t1 MTR_6g038240 30.323 155 93 3 6 160 10 149 1.47e-16 76.6
MS.gene86112.t1 MTR_7g013220 29.944 177 108 5 6 175 4 171 2.03e-16 76.3
MS.gene86112.t1 MTR_7g013240 28.994 169 106 4 6 174 5 159 2.22e-16 75.9
MS.gene86112.t1 MTR_7g013200 29.412 170 107 3 6 175 4 160 6.02e-16 74.7
MS.gene86112.t1 MTR_3g069710 31.928 166 93 5 9 173 14 160 1.94e-15 73.2
MS.gene86112.t1 MTR_4g064987 29.310 174 106 5 2 173 3 161 3.33e-15 72.8
MS.gene86112.t1 MTR_5g031860 26.374 182 102 4 4 174 9 169 5.46e-15 72.0
MS.gene86112.t1 MTR_5g031840 28.736 174 105 5 6 174 4 163 5.63e-15 72.0
MS.gene86112.t1 MTR_5g031830 25.275 182 104 4 4 174 9 169 3.66e-14 69.7
MS.gene86112.t1 MTR_7g013180 23.952 167 116 3 6 170 4 161 5.44e-14 69.3
MS.gene86112.t1 MTR_3g069690 29.932 147 84 4 9 155 10 137 9.82e-14 68.6
MS.gene86112.t1 MTR_7g106850 70.270 37 10 1 12 47 144 180 7.02e-13 63.9
MS.gene86112.t1 MTR_4g064987 29.012 162 97 6 2 161 3 148 1.33e-12 65.1
MS.gene86112.t1 MTR_5g072860 31.176 170 99 6 7 174 18 171 2.79e-12 64.3
MS.gene86112.t1 MTR_7g080935 31.757 148 84 5 4 151 8 138 1.10e-11 62.4
MS.gene86112.t1 MTR_6g038300 26.846 149 93 4 6 154 10 142 1.40e-11 62.4
MS.gene86112.t1 MTR_5g035580 28.205 156 91 6 7 160 13 149 2.01e-11 61.6
MS.gene86112.t1 MTR_5g004940 26.220 164 102 4 2 160 12 161 4.61e-11 60.5
MS.gene86112.t1 MTR_0477s0010 57.778 45 19 0 130 174 1 45 4.69e-11 60.5
MS.gene86112.t1 MTR_1g107315 28.070 171 106 6 2 165 3 163 7.26e-11 60.1
MS.gene86112.t1 MTR_5g098960 25.143 175 117 3 1 175 1 161 8.15e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86112.t1 AT1G22400 52.326 172 75 2 2 173 8 172 3.74e-59 192
MS.gene86112.t1 AT1G22370 52.941 170 73 2 4 173 10 172 7.35e-59 191
MS.gene86112.t1 AT1G22340 51.744 172 76 2 2 173 8 172 9.42e-58 188
MS.gene86112.t1 AT1G22380 51.744 172 76 2 2 173 8 172 2.42e-57 187
MS.gene86112.t1 AT1G78270 52.907 172 74 2 2 173 8 172 9.84e-57 185
MS.gene86112.t1 AT1G22360 52.353 170 74 2 4 173 7 169 1.60e-56 184
MS.gene86112.t1 AT1G22360 52.353 170 74 2 4 173 7 169 1.65e-56 184
MS.gene86112.t1 AT3G02100 34.483 174 101 6 5 174 11 175 2.84e-24 98.6
MS.gene86112.t1 AT3G46660 34.545 165 86 6 4 167 11 154 3.66e-20 87.0
MS.gene86112.t1 AT5G38010 34.104 173 100 4 2 173 5 164 2.10e-19 84.7
MS.gene86112.t1 AT3G46670 32.738 168 89 6 3 167 2 148 4.30e-18 80.9
MS.gene86112.t1 AT5G05890 34.302 172 92 5 7 173 8 163 6.16e-18 80.5
MS.gene86112.t1 AT5G05900 32.941 170 97 4 7 173 8 163 1.72e-17 79.3
MS.gene86112.t1 AT5G05860 34.524 168 89 7 7 172 9 157 3.57e-17 78.2
MS.gene86112.t1 AT3G11340 29.310 174 99 6 2 173 4 155 5.16e-16 75.1
MS.gene86112.t1 AT5G05880 32.335 167 98 4 7 173 8 159 9.18e-16 74.3
MS.gene86112.t1 AT3G46650 30.065 153 91 3 3 155 6 142 2.23e-14 70.5
MS.gene86112.t1 AT5G59580 31.126 151 87 4 5 155 7 140 2.26e-14 70.5
MS.gene86112.t1 AT3G21560 32.051 156 87 5 6 153 11 155 4.41e-14 69.7
MS.gene86112.t1 AT5G05870 33.728 169 94 7 7 172 8 161 5.64e-14 69.3
MS.gene86112.t1 AT2G36970 29.651 172 104 4 5 168 8 170 7.82e-14 68.9
MS.gene86112.t1 AT3G55700 28.902 173 96 6 9 173 10 163 2.03e-13 67.8
MS.gene86112.t1 AT5G59590 32.653 147 83 5 9 155 12 142 2.20e-13 67.4
MS.gene86112.t1 AT5G38040 30.952 168 82 6 15 173 18 160 2.33e-13 67.4
MS.gene86112.t1 AT3G46690 29.054 148 89 4 5 152 7 138 7.57e-12 63.2
MS.gene86112.t1 AT3G46720 29.139 151 90 3 2 152 4 137 1.38e-11 62.4
MS.gene86112.t1 AT2G30140 31.333 150 79 6 2 145 4 135 2.81e-11 61.2
MS.gene86112.t1 AT2G30140 31.333 150 79 6 2 145 4 135 2.98e-11 61.2
MS.gene86112.t1 AT4G15500 28.402 169 105 6 1 165 3 159 3.55e-11 61.2
MS.gene86112.t1 AT2G30150 31.250 160 83 6 1 152 1 141 6.98e-11 60.1

Find 32 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCCAATGCATGTACATTT+TGG 0.203508 6.3:+8954657 MS.gene86112:CDS
TTACTTACAATGTTTGATAA+AGG 0.314567 6.3:+8954692 MS.gene86112:CDS
GTTTGATTAATGGATTGATA+TGG 0.325659 6.3:-8954262 None:intergenic
ATATGGCCTTGAACTGGATA+TGG 0.341554 6.3:-8954245 None:intergenic
CTTAATGAATCTGCCACTTC+TGG 0.352562 6.3:+8954536 MS.gene86112:CDS
AAAGGTGTCATACCACTAAA+AGG 0.354121 6.3:+8954710 MS.gene86112:CDS
TCAGCAATTATGCACGTAAC+TGG 0.375098 6.3:-8954569 None:intergenic
AGGTTATGTGAAAGCCTCTA+AGG 0.390826 6.3:-8954301 None:intergenic
TAAGCGTTTGTGATTGTATT+CGG 0.416700 6.3:-8954333 None:intergenic
GCTGGACTAAAGAAAACAAC+AGG 0.434619 6.3:-8954638 None:intergenic
GTGAGACTATCAAAGGCATT+TGG 0.439338 6.3:-8954371 None:intergenic
TCCCCATCGCCGTCGATTGA+AGG 0.445545 6.3:-8954428 None:intergenic
TTAATTCCATATCCAGTTCA+AGG 0.449656 6.3:+8954239 MS.gene86112:CDS
TACCTTCAATCGACGGCGAT+GGG 0.461817 6.3:+8954426 MS.gene86112:CDS
GAAGTTCGCGAAAAGGTTGT+AGG 0.477376 6.3:-8954505 None:intergenic
GGATTGATATGGCCTTGAAC+TGG 0.479128 6.3:-8954251 None:intergenic
ACATTTGAGGCTATTCCTGA+TGG 0.479332 6.3:+8954401 MS.gene86112:CDS
TCGATTGAAGGTAGACCATC+AGG 0.487781 6.3:-8954416 None:intergenic
CAAAATGTACATGCATTGGC+TGG 0.536946 6.3:-8954656 None:intergenic
CTAGCAAGAAGTTCGCGAAA+AGG 0.539276 6.3:-8954512 None:intergenic
CTACCTTCAATCGACGGCGA+TGG 0.543140 6.3:+8954425 MS.gene86112:CDS
AGTCCAAAATGTACATGCAT+TGG 0.552178 6.3:-8954660 None:intergenic
TCAATCGACGGCGATGGGGA+TGG 0.572896 6.3:+8954431 MS.gene86112:CDS
TGGAGGCGCAAGACCAGAAG+TGG 0.580392 6.3:-8954549 None:intergenic
AAAATCAGTGAGACTATCAA+AGG 0.611232 6.3:-8954378 None:intergenic
ACCTTCAATCGACGGCGATG+GGG 0.622743 6.3:+8954427 MS.gene86112:CDS
GCAATTATGCACGTAACTGG+AGG 0.635156 6.3:-8954566 None:intergenic
ATTGTATTCGGTGTTGACAA+AGG 0.653219 6.3:-8954321 None:intergenic
GATGGTCTACCTTCAATCGA+CGG 0.659352 6.3:+8954419 MS.gene86112:CDS
TATGGAATTAACACTGCATG+TGG 0.667789 6.3:-8954227 None:intergenic
AAACGCTTACTCAAATCTAG+AGG 0.668124 6.3:+8954347 MS.gene86112:CDS
CTGGACTAAAGAAAACAACA+GGG 0.724710 6.3:-8954637 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATTCATATGACATATATA+AGG + chr6.3:8954783-8954802 MS.gene86112:intron 20.0%
!! TTACTTACAATGTTTGATAA+AGG + chr6.3:8954692-8954711 MS.gene86112:CDS 20.0%
!!! ATTTTTGCTAGTTTGATTAA+TGG - chr6.3:8954275-8954294 None:intergenic 20.0%
!! AATTGATTGTTACCTTTTAG+TGG - chr6.3:8954725-8954744 None:intergenic 25.0%
!!! AGTCTTCTTTTTTACTTCAT+AGG + chr6.3:8954745-8954764 MS.gene86112:intron 25.0%
!!! GTTTGATTAATGGATTGATA+TGG - chr6.3:8954265-8954284 None:intergenic 25.0%
AAAATCAGTGAGACTATCAA+AGG - chr6.3:8954381-8954400 None:intergenic 30.0%
TAAGCGTTTGTGATTGTATT+CGG - chr6.3:8954336-8954355 None:intergenic 30.0%
TTAATTCCATATCCAGTTCA+AGG + chr6.3:8954239-8954258 MS.gene86112:CDS 30.0%
!!! TCTCACTGATTTTACATTTG+AGG + chr6.3:8954388-8954407 MS.gene86112:CDS 30.0%
AAACGCTTACTCAAATCTAG+AGG + chr6.3:8954347-8954366 MS.gene86112:CDS 35.0%
AGTCCAAAATGTACATGCAT+TGG - chr6.3:8954663-8954682 None:intergenic 35.0%
CTGGACTAAAGAAAACAACA+GGG - chr6.3:8954640-8954659 None:intergenic 35.0%
GCAAAAATTCTTCACCTTAG+AGG + chr6.3:8954287-8954306 MS.gene86112:CDS 35.0%
TATGGAATTAACACTGCATG+TGG - chr6.3:8954230-8954249 None:intergenic 35.0%
! AATGCATGTACATTTTGGAC+TGG + chr6.3:8954662-8954681 MS.gene86112:CDS 35.0%
!! AAAGGTGTCATACCACTAAA+AGG + chr6.3:8954710-8954729 MS.gene86112:CDS 35.0%
!! ATTGTATTCGGTGTTGACAA+AGG - chr6.3:8954324-8954343 None:intergenic 35.0%
AGGTTATGTGAAAGCCTCTA+AGG - chr6.3:8954304-8954323 None:intergenic 40.0%
GCTGGACTAAAGAAAACAAC+AGG - chr6.3:8954641-8954660 None:intergenic 40.0%
TCAGCAATTATGCACGTAAC+TGG - chr6.3:8954572-8954591 None:intergenic 40.0%
! ACATTTGAGGCTATTCCTGA+TGG + chr6.3:8954401-8954420 MS.gene86112:CDS 40.0%
! ATATGGCCTTGAACTGGATA+TGG - chr6.3:8954248-8954267 None:intergenic 40.0%
! CAAAATGTACATGCATTGGC+TGG - chr6.3:8954659-8954678 None:intergenic 40.0%
! CAGCCAATGCATGTACATTT+TGG + chr6.3:8954657-8954676 MS.gene86112:CDS 40.0%
! CTTAATGAATCTGCCACTTC+TGG + chr6.3:8954536-8954555 MS.gene86112:CDS 40.0%
!! GTGAGACTATCAAAGGCATT+TGG - chr6.3:8954374-8954393 None:intergenic 40.0%
CTAGCAAGAAGTTCGCGAAA+AGG - chr6.3:8954515-8954534 None:intergenic 45.0%
GAAGTTCGCGAAAAGGTTGT+AGG - chr6.3:8954508-8954527 None:intergenic 45.0%
GATGGTCTACCTTCAATCGA+CGG + chr6.3:8954419-8954438 MS.gene86112:CDS 45.0%
GCAATTATGCACGTAACTGG+AGG - chr6.3:8954569-8954588 None:intergenic 45.0%
TCGATTGAAGGTAGACCATC+AGG - chr6.3:8954419-8954438 None:intergenic 45.0%
! GGATTGATATGGCCTTGAAC+TGG - chr6.3:8954254-8954273 None:intergenic 45.0%
TACCTTCAATCGACGGCGAT+GGG + chr6.3:8954426-8954445 MS.gene86112:CDS 50.0%
ACCTTCAATCGACGGCGATG+GGG + chr6.3:8954427-8954446 MS.gene86112:CDS 55.0%
CTACCTTCAATCGACGGCGA+TGG + chr6.3:8954425-8954444 MS.gene86112:CDS 55.0%
TCAATCGACGGCGATGGGGA+TGG + chr6.3:8954431-8954450 MS.gene86112:CDS 60.0%
! TCCCCATCGCCGTCGATTGA+AGG - chr6.3:8954431-8954450 None:intergenic 60.0%
! TGGAGGCGCAAGACCAGAAG+TGG - chr6.3:8954552-8954571 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 8954212 8954812 8954212 ID=MS.gene86112
chr6.3 mRNA 8954212 8954812 8954212 ID=MS.gene86112.t1;Parent=MS.gene86112
chr6.3 exon 8954212 8954731 8954212 ID=MS.gene86112.t1.exon1;Parent=MS.gene86112.t1
chr6.3 CDS 8954212 8954731 8954212 ID=cds.MS.gene86112.t1;Parent=MS.gene86112.t1
chr6.3 exon 8954805 8954812 8954805 ID=MS.gene86112.t1.exon2;Parent=MS.gene86112.t1
chr6.3 CDS 8954805 8954812 8954805 ID=cds.MS.gene86112.t1;Parent=MS.gene86112.t1
Gene Sequence

>MS.gene86112

ATGAATGATTCAAAACCACATGCAGTGTTAATTCCATATCCAGTTCAAGGCCATATCAATCCATTAATCAAACTAGCAAAAATTCTTCACCTTAGAGGCTTTCACATAACCTTTGTCAACACCGAATACAATCACAAACGCTTACTCAAATCTAGAGGTCCAAATGCCTTTGATAGTCTCACTGATTTTACATTTGAGGCTATTCCTGATGGTCTACCTTCAATCGACGGCGATGGGGATGGTGATGTTAGTCAAGACATATATGCTCTTTGTGAATCAATCAGAACGAATTTCCTACAACCTTTTCGCGAACTTCTTGCTAGACTTAATGAATCTGCCACTTCTGGTCTTGCGCCTCCAGTTACGTGCATAATTGCTGATAACAGCATGTCTTTTACTATACAAGCTGCTGAAGAAGTTTCAATCCCTGTTGTTTTCTTTAGTCCAGCCAATGCATGTACATTTTGGACTGGTTTTCACTTACTTACAATGTTTGATAAAGGTGTCATACCACTAAAAGGTAACAATCAATTAGTCTTCTTTTTTACTTCATAGGTGTAGTGTTGTTAAAGTATTCATATGACATATATAAGGAGTATAG

Protein sequence

>MS.gene86112.t1

MNDSKPHAVLIPYPVQGHINPLIKLAKILHLRGFHITFVNTEYNHKRLLKSRGPNAFDSLTDFTFEAIPDGLPSIDGDGDGDVSQDIYALCESIRTNFLQPFRELLARLNESATSGLAPPVTCIIADNSMSFTIQAAEEVSIPVVFFSPANACTFWTGFHLLTMFDKGVIPLKGV