AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86387

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86387.t1 MTR_2g103650 96.782 404 13 0 1 404 1 404 0.0 806
MS.gene86387.t1 MTR_5g043350 45.185 405 206 5 5 402 1 396 1.36e-118 350
MS.gene86387.t1 MTR_3g054320 46.684 377 185 5 28 402 30 392 1.73e-113 338
MS.gene86387.t1 MTR_3g084380 46.053 380 191 5 26 402 19 387 4.91e-111 331
MS.gene86387.t1 MTR_6g028080 44.094 381 196 6 27 402 29 397 1.17e-110 330
MS.gene86387.t1 MTR_4g084050 42.365 406 214 6 5 402 1 394 2.84e-108 324
MS.gene86387.t1 MTR_3g084360 41.500 400 222 4 5 402 1 390 4.06e-107 321
MS.gene86387.t1 MTR_8g010080 42.998 407 202 9 5 402 1 386 7.27e-105 315
MS.gene86387.t1 MTR_0157s0060 45.745 376 184 5 31 402 1 360 2.79e-103 310
MS.gene86387.t1 MTR_4g034020 45.745 376 184 5 31 402 1 360 2.79e-103 310
MS.gene86387.t1 MTR_8g010070 42.054 409 209 8 5 404 1 390 1.84e-101 306
MS.gene86387.t1 MTR_8g010040 43.272 379 194 8 14 391 18 376 3.56e-95 290
MS.gene86387.t1 MTR_1g012390 39.558 407 228 9 5 404 2 397 2.02e-94 289
MS.gene86387.t1 MTR_2g103410 42.077 366 206 5 28 392 59 419 5.18e-94 290
MS.gene86387.t1 MTR_8g008765 42.936 361 178 10 5 356 1 342 3.24e-93 284
MS.gene86387.t1 MTR_1g052315 41.547 349 197 4 23 370 59 401 1.90e-91 283
MS.gene86387.t1 MTR_8g010110 39.803 407 216 8 5 402 1 387 1.24e-90 279
MS.gene86387.t1 MTR_8g010090 38.725 408 219 10 5 402 1 387 2.68e-87 270
MS.gene86387.t1 MTR_8g065030 39.118 363 213 5 29 390 66 421 9.71e-87 271
MS.gene86387.t1 MTR_6g005630 38.021 384 222 10 23 404 40 409 3.93e-85 266
MS.gene86387.t1 MTR_4g094715 36.856 388 233 7 23 402 60 443 3.14e-84 265
MS.gene86387.t1 MTR_1g013740 36.548 394 218 7 9 393 8 378 1.04e-83 261
MS.gene86387.t1 MTR_7g091850 40.220 363 209 6 12 372 60 416 1.38e-83 263
MS.gene86387.t1 MTR_8g010050 38.084 407 190 8 5 402 1 354 8.70e-83 258
MS.gene86387.t1 MTR_7g091840 42.169 332 185 6 14 342 50 377 2.96e-81 254
MS.gene86387.t1 MTR_2g060350 40.525 343 196 5 30 370 76 412 2.94e-80 254
MS.gene86387.t1 MTR_1g086390 36.364 385 235 7 28 404 81 463 7.96e-79 251
MS.gene86387.t1 MTR_1g013730 34.860 393 229 7 5 393 1 370 8.56e-79 248
MS.gene86387.t1 MTR_8g077320 38.243 387 170 9 28 401 33 363 3.74e-78 246
MS.gene86387.t1 MTR_8g065010 35.784 408 227 10 24 402 103 504 7.67e-78 250
MS.gene86387.t1 MTR_3g438050 36.504 389 226 9 11 389 5 382 1.87e-77 245
MS.gene86387.t1 MTR_5g034090 35.222 406 229 10 25 402 118 517 2.18e-77 249
MS.gene86387.t1 MTR_7g035415 41.720 314 168 5 19 330 31 331 1.28e-76 241
MS.gene86387.t1 MTR_7g110050 34.197 386 241 7 28 404 67 448 3.73e-76 244
MS.gene86387.t1 MTR_5g033680 34.771 371 232 6 27 392 58 423 1.96e-75 242
MS.gene86387.t1 MTR_8g008700 41.325 317 146 7 90 404 14 292 6.87e-75 235
MS.gene86387.t1 MTR_3g030540 40.741 351 200 5 23 372 65 408 3.15e-74 238
MS.gene86387.t1 MTR_6g005630 36.458 384 205 11 23 404 40 386 2.38e-72 232
MS.gene86387.t1 MTR_2g032710 35.401 387 238 7 24 402 63 445 2.02e-71 231
MS.gene86387.t1 MTR_1g088160 36.695 357 215 7 28 381 35 383 3.86e-68 221
MS.gene86387.t1 MTR_4g120320 38.268 358 207 10 22 373 46 395 3.86e-68 222
MS.gene86387.t1 MTR_4g083660 37.726 387 224 9 20 402 1 374 1.82e-67 218
MS.gene86387.t1 MTR_2g049400 36.456 395 230 13 1 390 1 379 1.17e-66 217
MS.gene86387.t1 MTR_8g030930 34.635 384 230 9 28 402 27 398 3.44e-65 213
MS.gene86387.t1 MTR_2g103410 42.966 263 145 4 28 289 59 317 2.94e-64 209
MS.gene86387.t1 MTR_7g113910 37.829 304 153 5 91 393 74 342 7.53e-62 204
MS.gene86387.t1 MTR_8g009810 33.673 392 236 10 9 390 11 388 1.11e-61 204
MS.gene86387.t1 MTR_2g058670 34.324 370 233 7 9 373 3 367 1.96e-61 204
MS.gene86387.t1 MTR_2g058640 34.584 373 228 9 9 373 3 367 2.89e-61 203
MS.gene86387.t1 MTR_2g058840 33.245 379 223 9 9 373 3 365 8.54e-60 200
MS.gene86387.t1 MTR_2g435640 36.550 342 206 8 28 365 53 387 9.99e-60 200
MS.gene86387.t1 MTR_8g031180 41.077 297 137 8 91 380 15 280 5.80e-59 194
MS.gene86387.t1 MTR_5g063430 36.842 323 183 10 8 325 6 312 1.14e-58 194
MS.gene86387.t1 MTR_5g034090 36.770 291 154 6 25 289 118 404 3.31e-57 193
MS.gene86387.t1 MTR_2g029170 36.286 350 197 12 62 402 29 361 1.42e-56 190
MS.gene86387.t1 MTR_5g097860 33.138 341 209 10 13 346 10 338 1.15e-52 179
MS.gene86387.t1 MTR_2g100040 29.716 387 201 14 7 386 6 328 3.49e-39 144
MS.gene86387.t1 MTR_7g088550 28.155 309 170 11 32 305 54 345 2.20e-22 99.0
MS.gene86387.t1 MTR_1g100040 24.242 396 257 11 32 397 60 442 7.78e-22 97.4
MS.gene86387.t1 MTR_7g110710 23.821 424 256 15 27 396 42 452 1.55e-21 96.7
MS.gene86387.t1 MTR_7g110710 23.821 424 256 15 27 396 42 452 1.67e-21 96.3
MS.gene86387.t1 MTR_1g099850 24.129 373 230 11 32 359 59 423 5.31e-21 95.1
MS.gene86387.t1 MTR_1g100050 24.810 395 256 11 32 397 54 436 6.89e-21 94.7
MS.gene86387.t1 MTR_1g100060 24.194 372 229 10 32 358 97 460 1.60e-20 94.0
MS.gene86387.t1 MTR_4g124980 24.877 406 240 14 28 392 74 455 1.72e-20 93.6
MS.gene86387.t1 MTR_8g098840 24.800 375 221 13 29 354 45 407 1.91e-20 93.2
MS.gene86387.t1 MTR_2g042740 24.638 414 231 14 28 392 56 437 3.12e-19 89.7
MS.gene86387.t1 MTR_8g028725 23.649 444 278 12 2 397 11 441 4.09e-19 89.4
MS.gene86387.t1 MTR_7g078680 27.000 300 177 9 29 298 56 343 3.10e-17 83.6
MS.gene86387.t1 MTR_3g449590 26.168 321 188 9 27 314 46 350 3.88e-17 83.2
MS.gene86387.t1 MTR_1g086030 23.839 323 201 9 27 318 81 389 9.95e-17 82.4
MS.gene86387.t1 MTR_3g096330 26.101 318 185 13 29 312 77 378 1.01e-16 82.8
MS.gene86387.t1 MTR_5g090160 25.076 327 189 12 25 314 41 348 1.04e-16 82.0
MS.gene86387.t1 MTR_1g086030 27.523 218 134 5 115 318 78 285 4.57e-16 79.7
MS.gene86387.t1 MTR_4g120730 24.234 359 216 12 27 348 39 378 1.65e-13 72.4
MS.gene86387.t1 MTR_7g072360 33.607 122 59 1 166 287 29 128 1.71e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86387.t1 AT5G17200 43.488 430 192 8 8 402 6 419 5.36e-113 337
MS.gene86387.t1 AT5G27530 41.750 400 209 3 6 402 31 409 3.80e-106 321
MS.gene86387.t1 AT4G35670 41.895 401 217 4 5 402 1 388 1.89e-103 312
MS.gene86387.t1 AT5G44840 38.945 398 234 4 5 402 1 389 1.46e-100 304
MS.gene86387.t1 AT3G15720 39.698 398 222 7 10 402 4 388 1.07e-96 296
MS.gene86387.t1 AT3G15720 39.698 398 222 7 10 402 5 389 1.31e-96 296
MS.gene86387.t1 AT3G15720 39.698 398 219 8 10 402 4 385 3.38e-95 293
MS.gene86387.t1 AT1G80170 40.162 371 217 4 24 393 49 415 7.22e-93 286
MS.gene86387.t1 AT4G32380 40.286 350 199 2 42 389 41 382 1.97e-92 285
MS.gene86387.t1 AT1G56710 39.691 388 221 6 26 404 46 429 3.00e-89 277
MS.gene86387.t1 AT3G57510 40.751 373 210 8 11 380 52 416 1.07e-88 275
MS.gene86387.t1 AT5G44830 39.828 349 191 3 54 402 1 330 7.35e-88 270
MS.gene86387.t1 AT2G41850 42.105 361 199 7 16 373 56 409 1.82e-85 267
MS.gene86387.t1 AT1G70500 37.831 378 223 5 16 390 55 423 1.95e-85 268
MS.gene86387.t1 AT1G23460 38.292 363 216 5 29 390 44 399 3.91e-84 263
MS.gene86387.t1 AT1G23460 38.292 363 216 5 29 390 68 423 6.64e-84 264
MS.gene86387.t1 AT1G48100 36.923 390 234 7 23 404 75 460 2.17e-82 260
MS.gene86387.t1 AT5G14650 38.058 381 225 8 24 402 47 418 2.10e-81 256
MS.gene86387.t1 AT3G07970 39.542 349 204 5 23 370 66 408 4.50e-80 253
MS.gene86387.t1 AT1G02460 36.951 387 231 8 28 403 89 473 4.66e-80 254
MS.gene86387.t1 AT4G01890 36.132 393 236 9 22 402 59 448 4.26e-79 251
MS.gene86387.t1 AT1G10640 35.961 406 226 11 25 402 116 515 6.75e-79 253
MS.gene86387.t1 AT1G60590 35.437 412 229 10 22 402 117 522 3.93e-78 251
MS.gene86387.t1 AT3G15720 38.690 336 190 5 71 402 2 325 8.47e-78 246
MS.gene86387.t1 AT4G32380 35.920 348 176 2 42 389 41 341 1.50e-76 243
MS.gene86387.t1 AT1G05650 38.010 392 222 11 4 391 7 381 9.17e-76 241
MS.gene86387.t1 AT1G05660 35.840 399 238 10 8 402 8 392 6.26e-75 238
MS.gene86387.t1 AT3G26610 35.753 372 226 6 28 391 67 433 1.29e-74 240
MS.gene86387.t1 AT4G35670 43.003 293 151 4 113 402 2 281 8.07e-72 227
MS.gene86387.t1 AT2G43890 37.921 356 206 9 11 362 10 354 1.19e-71 230
MS.gene86387.t1 AT4G32370 38.066 331 188 6 1 322 4 326 2.14e-71 228
MS.gene86387.t1 AT2G43880 37.105 380 220 9 27 402 30 394 5.41e-71 228
MS.gene86387.t1 AT1G02790 38.012 342 194 9 26 365 50 375 1.78e-68 223
MS.gene86387.t1 AT1G78400 36.800 375 219 8 22 390 29 391 1.07e-67 220
MS.gene86387.t1 AT2G43860 38.281 384 218 12 26 404 31 400 3.31e-67 219
MS.gene86387.t1 AT1G65570 35.354 396 239 11 1 391 3 386 8.55e-67 218
MS.gene86387.t1 AT1G80170 42.697 267 148 4 24 289 49 311 1.30e-66 215
MS.gene86387.t1 AT1G43080 35.248 383 230 10 28 402 31 403 3.51e-66 216
MS.gene86387.t1 AT2G15460 36.096 374 219 11 28 392 31 393 7.81e-66 215
MS.gene86387.t1 AT1G43090 35.580 371 221 10 28 390 31 391 1.50e-65 216
MS.gene86387.t1 AT1G80140 35.882 340 206 4 66 402 6 336 1.79e-65 212
MS.gene86387.t1 AT2G15450 35.829 374 220 11 28 392 31 393 2.10e-65 214
MS.gene86387.t1 AT5G39910 33.690 374 187 5 4 375 13 327 2.70e-65 213
MS.gene86387.t1 AT4G32375 39.161 286 165 2 104 388 4 281 3.02e-65 214
MS.gene86387.t1 AT2G26620 35.040 371 225 9 28 392 31 391 4.03e-65 213
MS.gene86387.t1 AT2G43870 34.586 399 238 14 10 402 3 384 4.40e-65 213
MS.gene86387.t1 AT4G13760 36.416 346 201 10 28 365 31 365 9.75e-65 213
MS.gene86387.t1 AT2G40310 33.090 411 243 11 9 402 8 403 9.75e-65 213
MS.gene86387.t1 AT1G43100 35.310 371 222 10 28 390 31 391 6.27e-64 211
MS.gene86387.t1 AT2G15470 35.561 374 221 11 28 392 31 393 9.82e-64 210
MS.gene86387.t1 AT1G17150 33.498 406 254 10 2 402 7 401 1.09e-63 209
MS.gene86387.t1 AT3G59850 37.069 348 204 9 27 370 22 358 6.31e-63 207
MS.gene86387.t1 AT3G59850 37.249 349 202 10 27 370 66 402 1.03e-62 208
MS.gene86387.t1 AT4G18180 34.463 354 222 6 23 372 34 381 2.85e-62 206
MS.gene86387.t1 AT2G33160 35.215 372 220 10 28 390 32 391 4.06e-62 212
MS.gene86387.t1 AT3G07820 33.780 373 228 10 5 373 1 358 1.30e-58 196
MS.gene86387.t1 AT5G14650 40.226 266 152 4 24 289 47 305 2.42e-58 193
MS.gene86387.t1 AT5G48140 35.347 331 200 7 46 373 39 358 1.68e-57 193
MS.gene86387.t1 AT2G43880 38.000 300 175 7 27 325 30 319 1.17e-56 189
MS.gene86387.t1 AT3G07830 31.296 409 251 10 5 399 1 393 4.74e-55 187
MS.gene86387.t1 AT3G07840 33.515 367 225 10 10 373 9 359 2.25e-53 182
MS.gene86387.t1 AT3G07850 34.961 389 227 10 21 403 63 431 7.57e-53 182
MS.gene86387.t1 AT3G14040 35.248 383 222 10 27 403 70 432 9.15e-53 182
MS.gene86387.t1 AT2G23900 25.558 403 244 15 32 397 60 443 1.42e-24 105
MS.gene86387.t1 AT3G62110 24.938 401 266 10 27 401 42 433 1.13e-23 102
MS.gene86387.t1 AT3G62110 24.938 401 266 10 27 401 42 433 1.13e-23 102
MS.gene86387.t1 AT3G42950 26.225 408 232 13 28 392 66 447 2.23e-22 99.4
MS.gene86387.t1 AT3G48950 26.688 311 180 11 32 306 49 347 2.99e-22 98.6
MS.gene86387.t1 AT3G61490 28.333 300 173 9 29 298 159 446 6.17e-22 98.2
MS.gene86387.t1 AT3G61490 25.600 375 218 13 29 354 61 423 6.42e-22 97.8
MS.gene86387.t1 AT3G61490 25.600 375 218 13 29 354 46 408 8.80e-22 97.4
MS.gene86387.t1 AT3G61490 25.600 375 218 13 29 354 46 408 8.80e-22 97.4
MS.gene86387.t1 AT3G61490 25.600 375 218 13 29 354 46 408 8.80e-22 97.4
MS.gene86387.t1 AT4G33440 28.438 320 177 13 28 312 72 374 1.10e-20 94.0
MS.gene86387.t1 AT4G23500 23.861 373 223 12 32 355 72 432 6.83e-18 85.9
MS.gene86387.t1 AT5G41870 25.949 316 194 8 28 314 45 349 7.65e-17 82.4
MS.gene86387.t1 AT4G23820 26.183 317 189 10 29 314 42 344 1.85e-16 81.3
MS.gene86387.t1 AT1G19170 24.623 398 252 15 28 392 87 469 2.61e-15 78.2
MS.gene86387.t1 AT3G16850 23.164 354 202 11 27 329 39 373 2.83e-12 68.6

Find 91 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCAATCCATGTGTTTATT+TGG 0.080796 2.3:-1417108 None:intergenic
ATATCAATGCCATCAGTATT+AGG 0.225865 2.3:-1417663 None:intergenic
TGCTATCGATTTCAAATAAA+AGG 0.252766 2.3:+1417543 MS.gene86387:CDS
TTTGATTAACACAATAAAAT+TGG 0.261059 2.3:-1418341 None:intergenic
AACTCTAACAAGCTGCAAAA+AGG 0.263487 2.3:+1417599 MS.gene86387:CDS
ACCTCAATGTATATATAATT+AGG 0.293049 2.3:-1415597 None:intergenic
ACCAATCCATGTGTTTATTT+GGG 0.310797 2.3:-1417107 None:intergenic
TAAAATTGGTCAATCATAAT+GGG 0.312360 2.3:-1418327 None:intergenic
GGTTAGAGCACCCCACATTT+TGG 0.323169 2.3:-1418581 None:intergenic
GGTCAATCATAATGGGGTTA+TGG 0.323679 2.3:-1418320 None:intergenic
ACTTTCAATGTATTGCAATA+TGG 0.324810 2.3:+1415274 MS.gene86387:CDS
TGCAGCTTGTTAGAGTTATA+TGG 0.325682 2.3:-1417593 None:intergenic
ATAAAATTGGTCAATCATAA+TGG 0.333850 2.3:-1418328 None:intergenic
AAGGAGAAAACCTTCATCTT+AGG 0.335531 2.3:-1418639 None:intergenic
AAAACAAAATCGGAATATTC+AGG 0.348846 2.3:+1417082 MS.gene86387:CDS
ACGTTGACTGAAACATTAAC+TGG 0.363004 2.3:+1418093 MS.gene86387:CDS
CATGGACCACGGAAGGCTAT+TGG 0.371597 2.3:-1415570 None:intergenic
TTGAGCAAGCAGTTTAGAAT+TGG 0.374243 2.3:-1418699 None:intergenic
CTTAACTCTTTCATTGCAAC+TGG 0.374474 2.3:+1417711 MS.gene86387:CDS
TTTGTAGCATTGGATCATTA+GGG 0.380584 2.3:+1418022 MS.gene86387:intron
GTCAATCATAATGGGGTTAT+GGG 0.383263 2.3:-1418319 None:intergenic
AGGCTCTTCCATGAGCATTA+TGG 0.387155 2.3:-1418665 None:intergenic
TTAATTTACAGGAGAATCAT+TGG 0.387802 2.3:-1415317 None:intergenic
TATCAATGCCATCAGTATTA+GGG 0.410347 2.3:-1417662 None:intergenic
CAAGCAGTTTAGAATTGGTT+TGG 0.412319 2.3:-1418694 None:intergenic
GTGAATGGTTTAATTATAAG+TGG 0.419645 2.3:+1417139 MS.gene86387:CDS
AGTGGAAAGGGGACTGTTGA+TGG 0.421230 2.3:+1417157 MS.gene86387:CDS
AGAGTTATATGGCTTCTTGC+TGG 0.423641 2.3:-1417582 None:intergenic
GTGGTCCAGGACATGGTATC+AGG 0.443137 2.3:+1417896 MS.gene86387:CDS
ATGCACATCCTCTACAATAT+CGG 0.446275 2.3:-1418058 None:intergenic
AGAAATTTACTTACGGGTGG+TGG 0.450674 2.3:-1417220 None:intergenic
TGTTGCACCTAAAACAAAAT+CGG 0.452223 2.3:+1417072 MS.gene86387:CDS
CAGTTGCAATGAAAGAGTTA+AGG 0.457514 2.3:-1417710 None:intergenic
TCTCTCCAGGTGCAATTAAA+CGG 0.458759 2.3:-1417638 None:intergenic
GGTTTAATTATAAGTGGAAA+GGG 0.462142 2.3:+1417145 MS.gene86387:CDS
GTGAGTGATGTAACATATAG+AGG 0.462577 2.3:+1418510 MS.gene86387:CDS
GATGTGTGTCAAAGCAAATC+AGG 0.463712 2.3:+1415501 MS.gene86387:CDS
TCCTTCTGCCATAATGCTCA+TGG 0.470413 2.3:+1418657 MS.gene86387:CDS
GATTGCATTGCTATTAGTGC+TGG 0.472202 2.3:+1417841 MS.gene86387:CDS
ATTGGTTTCTCATTTGTGAA+TGG 0.475752 2.3:+1417124 MS.gene86387:CDS
ATCTGCTGTTCCTAAGATGA+AGG 0.477417 2.3:+1418629 MS.gene86387:CDS
CAGCTAAGACATTCTTATTG+AGG 0.482118 2.3:+1415547 MS.gene86387:CDS
TCCCAAATAAACACATGGAT+TGG 0.484532 2.3:+1417106 MS.gene86387:CDS
TATATATAATTAGGTTTGCA+TGG 0.485064 2.3:-1415588 None:intergenic
CTGAAACATTAACTGGAGTA+AGG 0.494577 2.3:+1418100 MS.gene86387:CDS
GTAACATATAGAGGGATTAG+TGG 0.503191 2.3:+1418519 MS.gene86387:CDS
AATAAGAATGTCTTAGCTGC+TGG 0.506230 2.3:-1415543 None:intergenic
GTATTGCAATATGGAGCAGT+TGG 0.517751 2.3:+1415283 MS.gene86387:CDS
CAATATGGAGCAGTTGGAGA+TGG 0.520589 2.3:+1415289 MS.gene86387:CDS
AACATCCGTTTAATTGCACC+TGG 0.524377 2.3:+1417633 MS.gene86387:CDS
CACAACAATGTTTCATACAA+TGG 0.531907 2.3:+1415053 None:intergenic
TGGTTTAATTATAAGTGGAA+AGG 0.535186 2.3:+1417144 MS.gene86387:CDS
TGTTTGCAACAGGGTGGAGG+TGG 0.535533 2.3:+1418252 MS.gene86387:intron
TCAGTATTAGGGCTCTCTCC+AGG 0.541986 2.3:-1417651 None:intergenic
TTTGAACTGCGACCAAAATG+TGG 0.542733 2.3:+1418569 MS.gene86387:CDS
TGTTGTTTGCAACAGGGTGG+AGG 0.545081 2.3:+1418249 MS.gene86387:intron
AGTGGTATAACATGTGGTCC+AGG 0.549585 2.3:+1417883 MS.gene86387:CDS
AAGGGGACTGTTGATGGTCG+AGG 0.551369 2.3:+1417163 MS.gene86387:CDS
AGGTTTGCATGGACCACGGA+AGG 0.551870 2.3:-1415577 None:intergenic
TCATCAGTAGTCAAAATTAG+TGG 0.554366 2.3:+1417865 MS.gene86387:CDS
CATAAACACGATCAAATACA+AGG 0.567940 2.3:-1418602 None:intergenic
TAGGTACTACTTACTGTTGG+TGG 0.572062 2.3:-1417374 None:intergenic
ATTATAAGTATGTTTAAACT+CGG 0.574801 2.3:+1415235 MS.gene86387:CDS
ACTAACCTGATACCATGTCC+TGG 0.574882 2.3:-1417901 None:intergenic
TAGAATTGGTTTGGTGTGTG+AGG 0.574986 2.3:-1418685 None:intergenic
AGTAAGGATCAAGACAAAGC+AGG 0.576165 2.3:+1418116 MS.gene86387:CDS
AGGGTGGAGGTGGATTTGCT+AGG 0.585416 2.3:+1418262 MS.gene86387:intron
GTTTAATTATAAGTGGAAAG+GGG 0.586309 2.3:+1417146 MS.gene86387:CDS
GGTGGTGGATTTCCTATGCA+CGG 0.586917 2.3:-1417205 None:intergenic
GTGGAGGTGGATTTGCTAGG+AGG 0.587173 2.3:+1418265 MS.gene86387:CDS
TGGTGGAAACAACCGTGCAT+AGG 0.588564 2.3:+1417193 MS.gene86387:CDS
TCCATGAGCATTATGGCAGA+AGG 0.604867 2.3:-1418658 None:intergenic
AAAATTAGTGGTATAACATG+TGG 0.605892 2.3:+1417877 MS.gene86387:CDS
TTGTAGCATTGGATCATTAG+GGG 0.609055 2.3:+1418023 MS.gene86387:intron
ATAACATGTGGTCCAGGACA+TGG 0.613113 2.3:+1417889 MS.gene86387:CDS
TGAGTGATGTAACATATAGA+GGG 0.613324 2.3:+1418511 MS.gene86387:CDS
CAGGATCCCAAATAAACACA+TGG 0.616392 2.3:+1417101 MS.gene86387:CDS
ACCTAATTATATATACATTG+AGG 0.616906 2.3:+1415596 MS.gene86387:CDS
GGAGAGAGCCCTAATACTGA+TGG 0.617288 2.3:+1417654 MS.gene86387:CDS
TTGAGGCCAATAGCCTTCCG+TGG 0.623914 2.3:+1415564 MS.gene86387:CDS
AAGGACTTGAATGTCTCTTG+AGG 0.626125 2.3:-1417691 None:intergenic
TACTACTTACTGTTGGTGGA+CGG 0.626963 2.3:-1417370 None:intergenic
TTGAACTGCGACCAAAATGT+GGG 0.634447 2.3:+1418570 MS.gene86387:CDS
AAAATTGGTCAATCATAATG+GGG 0.641528 2.3:-1418326 None:intergenic
TTGATGGTCGAGGCTCTATG+TGG 0.642714 2.3:+1417173 MS.gene86387:CDS
AATTAGGTTTGCATGGACCA+CGG 0.648764 2.3:-1415581 None:intergenic
ATGGTCGAGGCTCTATGTGG+TGG 0.666767 2.3:+1417176 MS.gene86387:CDS
TGGAGACACCGATATTGTAG+AGG 0.670467 2.3:+1418050 MS.gene86387:CDS
TGGAACATCATTAACAGACA+AGG 0.670604 2.3:+1418539 MS.gene86387:CDS
ATTGGATCATTAGGGGCACG+TGG 0.717529 2.3:+1418030 MS.gene86387:CDS
TGAACTGCGACCAAAATGTG+GGG 0.756518 2.3:+1418571 MS.gene86387:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAAAAATCTTAATTA+AGG - chr2.3:1418001-1418020 None:intergenic 10.0%
!!! AACAAATTTAATATTTTCAA+AGG - chr2.3:1418295-1418314 None:intergenic 10.0%
!!! TTAAATTATTTTATCACATT+TGG - chr2.3:1415432-1415451 None:intergenic 10.0%
!! AGTAACATATATAAGAAAAA+TGG - chr2.3:1418408-1418427 None:intergenic 15.0%
!! ATTATAAGTATGTTTAAACT+CGG + chr2.3:1415235-1415254 MS.gene86387:CDS 15.0%
!! CATATTTGTAAAAGAAAATA+TGG + chr2.3:1416448-1416467 MS.gene86387:intron 15.0%
!! TAGATATAGAAATTTACTTA+CGG - chr2.3:1417230-1417249 None:intergenic 15.0%
!! TTTGATTAACACAATAAAAT+TGG - chr2.3:1418344-1418363 None:intergenic 15.0%
!!! ATTTTATTGTGTTAATCAAA+TGG + chr2.3:1418344-1418363 MS.gene86387:CDS 15.0%
!!! TGCTATAATATTTTTTCAAT+AGG + chr2.3:1415458-1415477 MS.gene86387:intron 15.0%
!!! TTTTATCATTTTTCACATTA+TGG + chr2.3:1415098-1415117 MS.gene86387:intron 15.0%
!! AAAATAAATACCAGTTCATA+TGG - chr2.3:1417476-1417495 None:intergenic 20.0%
!! AATAGTTAAGTACTTTACAA+AGG + chr2.3:1416901-1416920 MS.gene86387:intron 20.0%
!! ACCTAATTATATATACATTG+AGG + chr2.3:1415596-1415615 MS.gene86387:CDS 20.0%
!! ACCTCAATGTATATATAATT+AGG - chr2.3:1415600-1415619 None:intergenic 20.0%
!! AGAGATCATAATTAATTTAC+AGG - chr2.3:1415331-1415350 None:intergenic 20.0%
!! AGATATAGAAATTTACTTAC+GGG - chr2.3:1417229-1417248 None:intergenic 20.0%
!! ATAAAATACTAGTCAACTAT+AGG - chr2.3:1416966-1416985 None:intergenic 20.0%
!! ATAAAATGTTAATTTCATGC+AGG + chr2.3:1415189-1415208 MS.gene86387:intron 20.0%
!! ATAAAATTGGTCAATCATAA+TGG - chr2.3:1418331-1418350 None:intergenic 20.0%
!! ATCAATTACCAATGTTATAT+TGG - chr2.3:1415968-1415987 None:intergenic 20.0%
!! ATGAAAAAAATATACTTGAG+AGG - chr2.3:1415888-1415907 None:intergenic 20.0%
!! ATTAATGGAAAGATCAATTA+AGG + chr2.3:1416400-1416419 MS.gene86387:intron 20.0%
!! ATTGTGTCTAATTATATTGT+AGG + chr2.3:1417814-1417833 MS.gene86387:intron 20.0%
!! TAAAATTGGTCAATCATAAT+GGG - chr2.3:1418330-1418349 None:intergenic 20.0%
!! TATATATAATTAGGTTTGCA+TGG - chr2.3:1415591-1415610 None:intergenic 20.0%
!! TTAATGGAAAGATCAATTAA+GGG + chr2.3:1416401-1416420 MS.gene86387:intron 20.0%
!! TTAATTGATCTTTCCATTAA+TGG - chr2.3:1416401-1416420 None:intergenic 20.0%
!!! ATAAGTGAGTTTTCATTTTT+TGG + chr2.3:1416818-1416837 MS.gene86387:intron 20.0%
!!! ATTGATGTGTTACTTTATTT+AGG + chr2.3:1415982-1416001 MS.gene86387:intron 20.0%
!!! ATTTATTTTTGTGTGTGTAT+GGG + chr2.3:1417487-1417506 MS.gene86387:intron 20.0%
!!! GAATATTTTTGTTTGTTTGT+TGG - chr2.3:1415753-1415772 None:intergenic 20.0%
!!! TATTTATTTTTGTGTGTGTA+TGG + chr2.3:1417486-1417505 MS.gene86387:intron 20.0%
!!! TTTTATAACCGTATTATTTC+TGG + chr2.3:1415829-1415848 MS.gene86387:intron 20.0%
!!! TTTTGAGGCAAATAATAAAA+TGG - chr2.3:1415356-1415375 None:intergenic 20.0%
!!! TTTTTGCTATATAATGGTTA+AGG - chr2.3:1416777-1416796 None:intergenic 20.0%
!!! TTTTTTCTTTTTTGTAGCAT+TGG + chr2.3:1418012-1418031 MS.gene86387:intron 20.0%
!!! TTTTTTTTTTGCCTTTTTCA+GGG + chr2.3:1417338-1417357 MS.gene86387:intron 20.0%
!!! TTTTTTTTTTTGCCTTTTTC+AGG + chr2.3:1417337-1417356 MS.gene86387:intron 20.0%
! AAAACAAAATCGGAATATTC+AGG + chr2.3:1417082-1417101 MS.gene86387:CDS 25.0%
! AAAATTAGTGGTATAACATG+TGG + chr2.3:1417877-1417896 MS.gene86387:CDS 25.0%
! AAAATTGGTCAATCATAATG+GGG - chr2.3:1418329-1418348 None:intergenic 25.0%
! AAAGGTTGTTATACAAAAGA+AGG + chr2.3:1416212-1416231 MS.gene86387:intron 25.0%
! AATACTTGCCAGAAATAATA+CGG - chr2.3:1415840-1415859 None:intergenic 25.0%
! ACAAGAGATAAATTCTCAAA+TGG + chr2.3:1416642-1416661 MS.gene86387:intron 25.0%
! ACTTTCAATGTATTGCAATA+TGG + chr2.3:1415274-1415293 MS.gene86387:CDS 25.0%
! AGTTCACAAAATAACACTAT+TGG + chr2.3:1418145-1418164 MS.gene86387:intron 25.0%
! ATGTGAAAAATGATAAAAGC+AGG - chr2.3:1415096-1415115 None:intergenic 25.0%
! CAGATCATAATCTTTAATGT+AGG + chr2.3:1416127-1416146 MS.gene86387:intron 25.0%
! CTACATTAAAGATTATGATC+TGG - chr2.3:1416129-1416148 None:intergenic 25.0%
! GGTTTAATTATAAGTGGAAA+GGG + chr2.3:1417145-1417164 MS.gene86387:CDS 25.0%
! GTGAATGGTTTAATTATAAG+TGG + chr2.3:1417139-1417158 MS.gene86387:CDS 25.0%
! GTTTAATTATAAGTGGAAAG+GGG + chr2.3:1417146-1417165 MS.gene86387:CDS 25.0%
! TGATCAATTACCTATAACAT+GGG - chr2.3:1416181-1416200 None:intergenic 25.0%
! TGATGCAAGTATTACTATAA+AGG - chr2.3:1417307-1417326 None:intergenic 25.0%
! TGGTTTAATTATAAGTGGAA+AGG + chr2.3:1417144-1417163 MS.gene86387:CDS 25.0%
! TTAATTTACAGGAGAATCAT+TGG - chr2.3:1415320-1415339 None:intergenic 25.0%
! TTGATCAAATAATCGCATAA+AGG + chr2.3:1416194-1416213 MS.gene86387:intron 25.0%
! TTGATCAATTACCTATAACA+TGG - chr2.3:1416182-1416201 None:intergenic 25.0%
!! CAATTAACTTGATGATACAT+TGG + chr2.3:1416669-1416688 MS.gene86387:intron 25.0%
!! TAGAGTTTTTGTATCTGAAT+AGG - chr2.3:1416287-1416306 None:intergenic 25.0%
!! TCTATAGTATGTATTTTGCA+AGG - chr2.3:1417413-1417432 None:intergenic 25.0%
!!! AGTTCACAAAAGTACTAAAT+AGG - chr2.3:1417035-1417054 None:intergenic 25.0%
!!! GAATATTCCGATTTTGTTTT+AGG - chr2.3:1417082-1417101 None:intergenic 25.0%
!!! TAACAAAGACATAATTTGCT+AGG + chr2.3:1416473-1416492 MS.gene86387:intron 25.0%
!!! TGAAATTTTTGGAACTTGAT+CGG + chr2.3:1415663-1415682 MS.gene86387:intron 25.0%
!!! TGCTATCGATTTCAAATAAA+AGG + chr2.3:1417543-1417562 MS.gene86387:CDS 25.0%
!!! TGTCACTTTTTGCTATATAA+TGG - chr2.3:1416783-1416802 None:intergenic 25.0%
!!! TTCTTCTAAAGTATACTATG+TGG + chr2.3:1416011-1416030 MS.gene86387:intron 25.0%
!!! TTTTTTTTTGCCTTTTTCAG+GGG + chr2.3:1417339-1417358 MS.gene86387:intron 25.0%
AACCAATCCATGTGTTTATT+TGG - chr2.3:1417111-1417130 None:intergenic 30.0%
ACCAATCCATGTGTTTATTT+GGG - chr2.3:1417110-1417129 None:intergenic 30.0%
AGACCAAAAGTTACAGATAT+AGG + chr2.3:1416052-1416071 MS.gene86387:intron 30.0%
AGAGCTATAAATACCACATT+TGG + chr2.3:1416242-1416261 MS.gene86387:intron 30.0%
ATATCAATGCCATCAGTATT+AGG - chr2.3:1417666-1417685 None:intergenic 30.0%
ATTGATCTTTCCATTAATGG+CGG - chr2.3:1416398-1416417 None:intergenic 30.0%
CAATGTTATATTGGTCAAAG+TGG - chr2.3:1415959-1415978 None:intergenic 30.0%
CACTTTGACCAATATAACAT+TGG + chr2.3:1415957-1415976 MS.gene86387:intron 30.0%
CATAAACACGATCAAATACA+AGG - chr2.3:1418605-1418624 None:intergenic 30.0%
GACCAAAAGTTACAGATATA+GGG + chr2.3:1416053-1416072 MS.gene86387:intron 30.0%
GAGCTATAAATACCACATTT+GGG + chr2.3:1416243-1416262 MS.gene86387:intron 30.0%
GATCAATTACCTATAACATG+GGG - chr2.3:1416180-1416199 None:intergenic 30.0%
TATAGAAATTTACTTACGGG+TGG - chr2.3:1417226-1417245 None:intergenic 30.0%
TATCAATGCCATCAGTATTA+GGG - chr2.3:1417665-1417684 None:intergenic 30.0%
TCATCAGTAGTCAAAATTAG+TGG + chr2.3:1417865-1417884 MS.gene86387:CDS 30.0%
TCCAAAAATTTCACTTGATG+AGG - chr2.3:1415656-1415675 None:intergenic 30.0%
TGAGTGATGTAACATATAGA+GGG + chr2.3:1418511-1418530 MS.gene86387:CDS 30.0%
TGTTGCACCTAAAACAAAAT+CGG + chr2.3:1417072-1417091 MS.gene86387:CDS 30.0%
TTCAACCAAAATGCATTGAA+AGG + chr2.3:1415770-1415789 MS.gene86387:intron 30.0%
! AATTGTATGTTGTTTGCAAC+AGG + chr2.3:1418242-1418261 MS.gene86387:intron 30.0%
! ACCTCATCAAGTGAAATTTT+TGG + chr2.3:1415652-1415671 MS.gene86387:intron 30.0%
! ATTGGTTTCTCATTTGTGAA+TGG + chr2.3:1417124-1417143 MS.gene86387:CDS 30.0%
! ATTGTATGTTGTTTGCAACA+GGG + chr2.3:1418243-1418262 MS.gene86387:intron 30.0%
! GAACTTGAGACTTTGTTTAA+GGG + chr2.3:1415919-1415938 MS.gene86387:intron 30.0%
! TGAACTTGAGACTTTGTTTA+AGG + chr2.3:1415918-1415937 MS.gene86387:intron 30.0%
! TGGTATTTATAGCTCTTTTC+TGG - chr2.3:1416238-1416257 None:intergenic 30.0%
! TTGTTTGTTTGTTGGAGTAT+TGG - chr2.3:1415745-1415764 None:intergenic 30.0%
! TTTGTAGCATTGGATCATTA+GGG + chr2.3:1418022-1418041 MS.gene86387:intron 30.0%
!! GTTTTAGTCTCTTAACTATG+TGG + chr2.3:1416579-1416598 MS.gene86387:intron 30.0%
!! TTTTGTAGCATTGGATCATT+AGG + chr2.3:1418021-1418040 MS.gene86387:intron 30.0%
!!! ACATGCAAAAGAGTATTTTG+AGG - chr2.3:1415371-1415390 None:intergenic 30.0%
!!! TTTTGGTCTTATCCGAATTA+CGG - chr2.3:1416041-1416060 None:intergenic 30.0%
AAATGATTCACCGCCATTAA+TGG + chr2.3:1416385-1416404 MS.gene86387:intron 35.0%
AACTCTAACAAGCTGCAAAA+AGG + chr2.3:1417599-1417618 MS.gene86387:CDS 35.0%
AAGGAGAAAACCTTCATCTT+AGG - chr2.3:1418642-1418661 None:intergenic 35.0%
AATAAGAATGTCTTAGCTGC+TGG - chr2.3:1415546-1415565 None:intergenic 35.0%
ACCAAAATGCATTGAAAGGT+TGG + chr2.3:1415774-1415793 MS.gene86387:intron 35.0%
ACTCTAACAAGCTGCAAAAA+GGG + chr2.3:1417600-1417619 MS.gene86387:CDS 35.0%
AGTCTCAAGTTCAAACATTG+TGG - chr2.3:1415911-1415930 None:intergenic 35.0%
ATCTGAATAGGTCATGTCTT+GGG - chr2.3:1416275-1416294 None:intergenic 35.0%
ATGCACATCCTCTACAATAT+CGG - chr2.3:1418061-1418080 None:intergenic 35.0%
CAGCTAAGACATTCTTATTG+AGG + chr2.3:1415547-1415566 MS.gene86387:CDS 35.0%
CAGTTGCAATGAAAGAGTTA+AGG - chr2.3:1417713-1417732 None:intergenic 35.0%
CTATAGGTTGTTCTACTTTC+TGG - chr2.3:1416950-1416969 None:intergenic 35.0%
CTATATGTACCCCATGTTAT+AGG + chr2.3:1416168-1416187 MS.gene86387:intron 35.0%
CTGAAACATTAACTGGAGTA+AGG + chr2.3:1418100-1418119 MS.gene86387:CDS 35.0%
CTTAACTCTTTCATTGCAAC+TGG + chr2.3:1417711-1417730 MS.gene86387:CDS 35.0%
GTAACATATAGAGGGATTAG+TGG + chr2.3:1418519-1418538 MS.gene86387:CDS 35.0%
GTATGGGATATATGTACAAC+AGG + chr2.3:1417503-1417522 MS.gene86387:intron 35.0%
GTGAGTGATGTAACATATAG+AGG + chr2.3:1418510-1418529 MS.gene86387:CDS 35.0%
TATCTGAATAGGTCATGTCT+TGG - chr2.3:1416276-1416295 None:intergenic 35.0%
TCAAATGGTCTGCAAAAACA+TGG + chr2.3:1418359-1418378 MS.gene86387:CDS 35.0%
TCCCAAATAAACACATGGAT+TGG + chr2.3:1417106-1417125 MS.gene86387:CDS 35.0%
TCTCTTAACTATGTGGTCTA+TGG + chr2.3:1416586-1416605 MS.gene86387:intron 35.0%
TGGAACATCATTAACAGACA+AGG + chr2.3:1418539-1418558 MS.gene86387:CDS 35.0%
TTTGAGAGATAAGGATGAGA+GGG - chr2.3:1416102-1416121 None:intergenic 35.0%
! ACGTTGACTGAAACATTAAC+TGG + chr2.3:1418093-1418112 MS.gene86387:CDS 35.0%
! CAAGCAGTTTAGAATTGGTT+TGG - chr2.3:1418697-1418716 None:intergenic 35.0%
! GTCAATCATAATGGGGTTAT+GGG - chr2.3:1418322-1418341 None:intergenic 35.0%
! TCCAACCTTTCAATGCATTT+TGG - chr2.3:1415778-1415797 None:intergenic 35.0%
! TTGAGCAAGCAGTTTAGAAT+TGG - chr2.3:1418702-1418721 None:intergenic 35.0%
! TTGTAGCATTGGATCATTAG+GGG + chr2.3:1418023-1418042 MS.gene86387:intron 35.0%
! TTTTGTGAACTCAGCTTTCA+GGG + chr2.3:1417044-1417063 MS.gene86387:intron 35.0%
!! AATAGGCTTTTCTGAAAGCA+TGG + chr2.3:1415475-1415494 MS.gene86387:intron 35.0%
!! TGCAGCTTGTTAGAGTTATA+TGG - chr2.3:1417596-1417615 None:intergenic 35.0%
!! TTTTGAGAGATAAGGATGAG+AGG - chr2.3:1416103-1416122 None:intergenic 35.0%
!!! AATTTTTGGAACTTGATCGG+TGG + chr2.3:1415666-1415685 MS.gene86387:intron 35.0%
!!! TCTGGTTGTTTTGAGAGATA+AGG - chr2.3:1416111-1416130 None:intergenic 35.0%
AACATCCGTTTAATTGCACC+TGG + chr2.3:1417633-1417652 MS.gene86387:CDS 40.0%
AAGGACTTGAATGTCTCTTG+AGG - chr2.3:1417694-1417713 None:intergenic 40.0%
AATAGAGAAGCTCAGTTGGA+AGG - chr2.3:1416323-1416342 None:intergenic 40.0%
AATTAGGTTTGCATGGACCA+CGG - chr2.3:1415584-1415603 None:intergenic 40.0%
AGAAATTTACTTACGGGTGG+TGG - chr2.3:1417223-1417242 None:intergenic 40.0%
AGAGTTATATGGCTTCTTGC+TGG - chr2.3:1417585-1417604 None:intergenic 40.0%
AGTAAGGATCAAGACAAAGC+AGG + chr2.3:1418116-1418135 MS.gene86387:CDS 40.0%
ATCTGCTGTTCCTAAGATGA+AGG + chr2.3:1418629-1418648 MS.gene86387:CDS 40.0%
CAGGATCCCAAATAAACACA+TGG + chr2.3:1417101-1417120 MS.gene86387:CDS 40.0%
CTGTTACGTACCATATGAAC+TGG + chr2.3:1417463-1417482 MS.gene86387:intron 40.0%
GAGTAGGTACTACTTACTGT+TGG - chr2.3:1417380-1417399 None:intergenic 40.0%
GATGTGTGTCAAAGCAAATC+AGG + chr2.3:1415501-1415520 MS.gene86387:CDS 40.0%
GCAAGGATCAATACAAGAGT+AGG - chr2.3:1417396-1417415 None:intergenic 40.0%
GTACAATAGAGAAGCTCAGT+TGG - chr2.3:1416327-1416346 None:intergenic 40.0%
GTATTGCAATATGGAGCAGT+TGG + chr2.3:1415283-1415302 MS.gene86387:CDS 40.0%
TAGGTACTACTTACTGTTGG+TGG - chr2.3:1417377-1417396 None:intergenic 40.0%
TCTCTCCAGGTGCAATTAAA+CGG - chr2.3:1417641-1417660 None:intergenic 40.0%
TTGAACTGCGACCAAAATGT+GGG + chr2.3:1418570-1418589 MS.gene86387:CDS 40.0%
TTTGAACTGCGACCAAAATG+TGG + chr2.3:1418569-1418588 MS.gene86387:CDS 40.0%
! CGCCCTATATCTGTAACTTT+TGG - chr2.3:1416058-1416077 None:intergenic 40.0%
! CTTTTGTGAACTCAGCTTTC+AGG + chr2.3:1417043-1417062 MS.gene86387:intron 40.0%
! GATTGCATTGCTATTAGTGC+TGG + chr2.3:1417841-1417860 MS.gene86387:CDS 40.0%
! GGTCAATCATAATGGGGTTA+TGG - chr2.3:1418323-1418342 None:intergenic 40.0%
! TACTACTTACTGTTGGTGGA+CGG - chr2.3:1417373-1417392 None:intergenic 40.0%
!! TAGAATTGGTTTGGTGTGTG+AGG - chr2.3:1418688-1418707 None:intergenic 40.0%
ACTAACCTGATACCATGTCC+TGG - chr2.3:1417904-1417923 None:intergenic 45.0%
AGGCTCTTCCATGAGCATTA+TGG - chr2.3:1418668-1418687 None:intergenic 45.0%
AGTGGTATAACATGTGGTCC+AGG + chr2.3:1417883-1417902 MS.gene86387:CDS 45.0%
ATAACATGTGGTCCAGGACA+TGG + chr2.3:1417889-1417908 MS.gene86387:CDS 45.0%
CAATATGGAGCAGTTGGAGA+TGG + chr2.3:1415289-1415308 MS.gene86387:CDS 45.0%
CTTGGGAATGATCCCAAATG+TGG - chr2.3:1416258-1416277 None:intergenic 45.0%
TCCATGAGCATTATGGCAGA+AGG - chr2.3:1418661-1418680 None:intergenic 45.0%
TCCTTCTGCCATAATGCTCA+TGG + chr2.3:1418657-1418676 MS.gene86387:CDS 45.0%
TGAACTGCGACCAAAATGTG+GGG + chr2.3:1418571-1418590 MS.gene86387:CDS 45.0%
TGGAGACACCGATATTGTAG+AGG + chr2.3:1418050-1418069 MS.gene86387:CDS 45.0%
! GTATGTTGTTTGCAACAGGG+TGG + chr2.3:1418246-1418265 MS.gene86387:intron 45.0%
!! TGGTCTTATCCGAATTACGG+CGG - chr2.3:1416038-1416057 None:intergenic 45.0%
AGTGGAAAGGGGACTGTTGA+TGG + chr2.3:1417157-1417176 MS.gene86387:CDS 50.0%
CTATGTGGACCGCCGTAATT+CGG + chr2.3:1416026-1416045 MS.gene86387:intron 50.0%
GAGAGATAAGGATGAGAGGG+CGG - chr2.3:1416099-1416118 None:intergenic 50.0%
GGAGAGAGCCCTAATACTGA+TGG + chr2.3:1417654-1417673 MS.gene86387:CDS 50.0%
GGCAACTTGTCCCCTGAAAA+AGG - chr2.3:1417352-1417371 None:intergenic 50.0%
TCAGTATTAGGGCTCTCTCC+AGG - chr2.3:1417654-1417673 None:intergenic 50.0%
TGGTGGAAACAACCGTGCAT+AGG + chr2.3:1417193-1417212 MS.gene86387:CDS 50.0%
! ATTGGATCATTAGGGGCACG+TGG + chr2.3:1418030-1418049 MS.gene86387:CDS 50.0%
! TGTTGTTTGCAACAGGGTGG+AGG + chr2.3:1418249-1418268 MS.gene86387:intron 50.0%
! TTGATGGTCGAGGCTCTATG+TGG + chr2.3:1417173-1417192 MS.gene86387:CDS 50.0%
!! GGTGGTGGATTTCCTATGCA+CGG - chr2.3:1417208-1417227 None:intergenic 50.0%
!! GGTTAGAGCACCCCACATTT+TGG - chr2.3:1418584-1418603 None:intergenic 50.0%
AGGTTTGCATGGACCACGGA+AGG - chr2.3:1415580-1415599 None:intergenic 55.0%
ATGGTCGAGGCTCTATGTGG+TGG + chr2.3:1417176-1417195 MS.gene86387:CDS 55.0%
CATGGACCACGGAAGGCTAT+TGG - chr2.3:1415573-1415592 None:intergenic 55.0%
GTGGTCCAGGACATGGTATC+AGG + chr2.3:1417896-1417915 MS.gene86387:CDS 55.0%
TGTTTGCAACAGGGTGGAGG+TGG + chr2.3:1418252-1418271 MS.gene86387:intron 55.0%
TTGAGGCCAATAGCCTTCCG+TGG + chr2.3:1415564-1415583 MS.gene86387:CDS 55.0%
! AGGGTGGAGGTGGATTTGCT+AGG + chr2.3:1418262-1418281 MS.gene86387:intron 55.0%
!! AAGGGGACTGTTGATGGTCG+AGG + chr2.3:1417163-1417182 MS.gene86387:CDS 55.0%
!! GTGGAGGTGGATTTGCTAGG+AGG + chr2.3:1418265-1418284 MS.gene86387:CDS 55.0%
GAGGGCGGCCTCATGGATTT+AGG - chr2.3:1416084-1416103 None:intergenic 60.0%
GGCGGCCTCATGGATTTAGG+CGG - chr2.3:1416081-1416100 None:intergenic 60.0%
AGGATGAGAGGGCGGCCTCA+TGG - chr2.3:1416091-1416110 None:intergenic 65.0%
! GGGCGCCGCCTAAATCCATG+AGG + chr2.3:1416073-1416092 MS.gene86387:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 1415060 1418725 1415060 ID=MS.gene86387
chr2.3 mRNA 1415060 1418725 1415060 ID=MS.gene86387.t1;Parent=MS.gene86387
chr2.3 exon 1415060 1415074 1415060 ID=MS.gene86387.t1.exon1;Parent=MS.gene86387.t1
chr2.3 CDS 1415060 1415074 1415060 ID=cds.MS.gene86387.t1;Parent=MS.gene86387.t1
chr2.3 exon 1415211 1415330 1415211 ID=MS.gene86387.t1.exon2;Parent=MS.gene86387.t1
chr2.3 CDS 1415211 1415330 1415211 ID=cds.MS.gene86387.t1;Parent=MS.gene86387.t1
chr2.3 exon 1415480 1415617 1415480 ID=MS.gene86387.t1.exon3;Parent=MS.gene86387.t1
chr2.3 CDS 1415480 1415617 1415480 ID=cds.MS.gene86387.t1;Parent=MS.gene86387.t1
chr2.3 exon 1417058 1417228 1417058 ID=MS.gene86387.t1.exon4;Parent=MS.gene86387.t1
chr2.3 CDS 1417058 1417228 1417058 ID=cds.MS.gene86387.t1;Parent=MS.gene86387.t1
chr2.3 exon 1417359 1417382 1417359 ID=MS.gene86387.t1.exon5;Parent=MS.gene86387.t1
chr2.3 CDS 1417359 1417382 1417359 ID=cds.MS.gene86387.t1;Parent=MS.gene86387.t1
chr2.3 exon 1417525 1417732 1417525 ID=MS.gene86387.t1.exon6;Parent=MS.gene86387.t1
chr2.3 CDS 1417525 1417732 1417525 ID=cds.MS.gene86387.t1;Parent=MS.gene86387.t1
chr2.3 exon 1417836 1417917 1417836 ID=MS.gene86387.t1.exon7;Parent=MS.gene86387.t1
chr2.3 CDS 1417836 1417917 1417836 ID=cds.MS.gene86387.t1;Parent=MS.gene86387.t1
chr2.3 exon 1418029 1418137 1418029 ID=MS.gene86387.t1.exon8;Parent=MS.gene86387.t1
chr2.3 CDS 1418029 1418137 1418029 ID=cds.MS.gene86387.t1;Parent=MS.gene86387.t1
chr2.3 exon 1418264 1418380 1418264 ID=MS.gene86387.t1.exon9;Parent=MS.gene86387.t1
chr2.3 CDS 1418264 1418380 1418264 ID=cds.MS.gene86387.t1;Parent=MS.gene86387.t1
chr2.3 exon 1418495 1418725 1418495 ID=MS.gene86387.t1.exon10;Parent=MS.gene86387.t1
chr2.3 CDS 1418495 1418725 1418495 ID=cds.MS.gene86387.t1;Parent=MS.gene86387.t1
Gene Sequence

>MS.gene86387

ATGTTTCATACAATGGTTAGGATAAGACATAATCCTGCTTTTATCATTTTTCACATTATGGTTTTAATCAAACATATATGTCATTATGTTTATGTGTGTTGATTTTTTTTTTAATCAAATGCACTTACTATAAAATGTTAATTTCATGCAGGAGTGTTTTTTCACATTGTTAATGATTATAAGTATGTTTAAACTCGGATTGAGTAGCACAACAACTTTCAATGTATTGCAATATGGAGCAGTTGGAGATGGAAAAACCAATGATTCTCCTGTAAATTAATTATGATCTCTCACCATTTTATTATTTGCCTCAAAATACTCTTTTGCATGTGTAACATTAATAACTTTAATTATTTCATTTGCTCAATTCCAAATGTGATAAAATAATTTAAAGTTTATGCTATAATATTTTTTCAATAGGCTTTTCTGAAAGCATGGAAAGATGTGTGTCAAAGCAAATCAGGCACATCTCGTCTCATCATACCAGCAGCTAAGACATTCTTATTGAGGCCAATAGCCTTCCGTGGTCCATGCAAACCTAATTATATATACATTGAGGTACTTATTAATTTGATTATTTTTGCAATATACAACCTCATCAAGTGAAATTTTTGGAACTTGATCGGTGGCTATAACTTAAAAGCATAGTAAACATTAACGTCAGTACTGTTTCAACTTTCAACCAATACTCCAACAAACAAACAAAAATATTCAACCAAAATGCATTGAAAGGTTGGAATTTGATAAAATTGATGTGTTATTTTAGTTTTTTTATAACCGTATTATTTCTGGCAAGTATTCACATTAAGACTTAAGTTGATCTCACCTCTCAAGTATATTTTTTTCATCCACAATGTTTGAACTTGAGACTTTGTTTAAGGGATTCAAGTTCAATTCCACTTTGACCAATATAACATTGGTAATTGATGTGTTACTTTATTTAGGATCTAGTTCTTCTAAAGTATACTATGTGGACCGCCGTAATTCGGATAAGACCAAAAGTTACAGATATAGGGCGCCGCCTAAATCCATGAGGCCGCCCTCTCATCCTTATCTCTCAAAACAACCAGATCATAATCTTTAATGTAGGATATTCAGACAGAGATGACTATATGTACCCCATGTTATAGGTAATTGATCAAATAATCGCATAAAGGTTGTTATACAAAAGAAGGCCAGAAAAGAGCTATAAATACCACATTTGGGATCATTCCCAAGACATGACCTATTCAGATACAAAAACTCTAATCATTCAGTTAACCTTCCAACTGAGCTTCTCTATTGTACTCACTTCAAGTAGACTATGAAAAGAAAAATGAGTTATGAAATGATTCACCGCCATTAATGGAAAGATCAATTAAGGGAATGCATTTTAAGTTTAACAAATTCATATTTGTAAAAGAAAATATGGTTTAACAAAGACATAATTTGCTAGGAATGAAAAAAGTGATATCAACAAACATACATTGTGTCTCTTTCAAAAAAAAAACAAACATACATTGTGTCACAAATTGTCGATGTTTTAGTCTCTTAACTATGTGGTCTATGGTTCAATTTATTATCAAAGCATATGAAAAAACATACAAGAGATAAATTCTCAAATGGATATCAATTAACTTGATGATACATTGGTTTTAAAAAAAAAAAAAATTATACAGTGTCTTCAAGACATTGATTAAGCAAGCAAAACAAATAAGTAATTTTTTTATAAATTCCTTAACCATTATATAGCAAAAAGTGACATGAGTGATGTTCATCATAAGTGAGTTTTCATTTTTTGGTATTTTTCTATTCAAAAGTCAAAGCTCGCTTTTAGATAAGTTAAATTCATTAAAAGTACGAATAGTTAAGTACTTTACAAAGGAATAAAAAAAGAACAAAAATAATCCAGAAAGTAGAACAACCTATAGTTGACTAGTATTTTATAATTTTATTTGAATTAAATTGAGAACTCAGCTTTGAGTTAAAGTAACCTATTTAGTACTTTTGTGAACTCAGCTTTCAGGGAATATTGTTGCACCTAAAACAAAATCGGAATATTCAGGATCCCAAATAAACACATGGATTGGTTTCTCATTTGTGAATGGTTTAATTATAAGTGGAAAGGGGACTGTTGATGGTCGAGGCTCTATGTGGTGGAAACAACCGTGCATAGGAAATCCACCACCCGTAAGTAAATTTCTATATCTATAGTTTTTTTAACTTAACATTTCAACTTATGTTACTTAAATAATACACTATATACCTTTATAGTAATACTTGCATCATTTTTATGATTTTTTTTTTTTGCCTTTTTCAGGGGACAAGTTGCCGTCCACCAACAGTAAGTAGTACCTACTCTTGTATTGATCCTTGCAAAATACATACTATAGACAATATTTTTGTTACAAAATTAAATTTTAGCTGTTACGTACCATATGAACTGGTATTTATTTTTGTGTGTGTATGGGATATATGTACAACAGGCAATAACATTGAATAGATGCTATCGATTTCAAATAAAAGGATATACAAGTGTTAATCCAGCAAGAAGCCATATAACTCTAACAAGCTGCAAAAAGGGCATTATCTCTAACATCCGTTTAATTGCACCTGGAGAGAGCCCTAATACTGATGGCATTGATATTTCTGCCTCAAGAGACATTCAAGTCCTTAACTCTTTCATTGCAACTGGTAATGTTTTAATAATTTGAACTCATTTCAATCTATATTAATTATTAATTAGATCTTAGTGTATGTAAAATAATCATCTTGATTGTGTCTAATTATATTGTAGGTGATGATTGCATTGCTATTAGTGCTGGATCATCAGTAGTCAAAATTAGTGGTATAACATGTGGTCCAGGACATGGTATCAGGTTAGTACTTCACTATAATTATAATAATGTAATTGAGTTTTTGAATTTTAATTTTTAATTAAAATATTCTAGCTACTTTACCTTAATTAAGATTTTTTTTCTTTTTTGTAGCATTGGATCATTAGGGGCACGTGGAGACACCGATATTGTAGAGGATGTGCATGTGAAGAATTGTACGTTGACTGAAACATTAACTGGAGTAAGGATCAAGACAAAGCAGGTAAATAAGTTCACAAAATAACACTATTGGTCACATGACTTAATTTATTTAAAAATTAACAAAAATAAAAAAATTATTTTAAATTTAATCTTTCAATCAATTTCAATTGTATGTTGTTTGCAACAGGGTGGAGGTGGATTTGCTAGGAGGATCACCTTTGAAAATATTAAATTTGTTCGAGCCCATAACCCCATTATGATTGACCAATTTTATTGTGTTAATCAAATGGTCTGCAAAAACATGGTAATTCAAGATAAATCATCAATTCCATTTTTCTTATATATGTTACTCTCTAAAAAATAAATTAATTTTCTACAACTTTTAACAAAAAATTTTATTGAATGTTGTTATGTGTAGACTAAAGCAATCAAAGTGAGTGATGTAACATATAGAGGGATTAGTGGAACATCATTAACAGACAAGGCAATAAATTTGAACTGCGACCAAAATGTGGGGTGCTCTAACCTTGTATTTGATCGTGTTTATGTAAGATCTGCTGTTCCTAAGATGAAGGTTTTCTCCTTCTGCCATAATGCTCATGGAAGAGCCTCACACACCAAACCAATTCTAAACTGCTTGCTCAAGTAG
Protein sequence

>MS.gene86387.t1

MFHTMECFFTLLMIISMFKLGLSSTTTFNVLQYGAVGDGKTNDSPAFLKAWKDVCQSKSGTSRLIIPAAKTFLLRPIAFRGPCKPNYIYIELSGNIVAPKTKSEYSGSQINTWIGFSFVNGLIISGKGTVDGRGSMWWKQPCIGNPPPGTSCRPPTAITLNRCYRFQIKGYTSVNPARSHITLTSCKKGIISNIRLIAPGESPNTDGIDISASRDIQVLNSFIATGDDCIAISAGSSVVKISGITCGPGHGISIGSLGARGDTDIVEDVHVKNCTLTETLTGVRIKTKQGGGGFARRITFENIKFVRAHNPIMIDQFYCVNQMVCKNMTKAIKVSDVTYRGISGTSLTDKAINLNCDQNVGCSNLVFDRVYVRSAVPKMKVFSFCHNAHGRASHTKPILNCLLK