Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049203 | MS.gene87177 | 0.804543 | 2.05E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene87177 | 0.814674 | 1.37E-51 | -1.69E-46 |
| MS.gene051594 | MS.gene87177 | 0.814269 | 1.68E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene87177 | 0.80979 | 1.59E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene87177 | 0.804233 | 2.38E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene87177 | 0.840122 | 1.05E-57 | -1.69E-46 |
| MS.gene056050 | MS.gene87177 | 0.805108 | 1.56E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene87177 | 0.81667 | 4.91E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 122 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCCAAATCTTCTCATTT+TGG | 0.143116 | 7.4:+65448097 | None:intergenic |
| AACTTTGTCTCTAGGCTTGC+AGG | 0.274208 | 7.4:-65449654 | MS.gene87177:intron |
| AGCGCCAAAATGAGAAGATT+TGG | 0.293225 | 7.4:-65448101 | MS.gene87177:CDS |
| TTCTGTGGCTGCAGTGGCTG+TGG | 0.414645 | 7.4:-65448401 | MS.gene87177:intron |
| GCGCCAAAATGAGAAGATTT+GGG | 0.435664 | 7.4:-65448100 | MS.gene87177:CDS |
| TAAGTGAGGCGGCAGGGCCG+TGG | 0.445627 | 7.4:-65448244 | MS.gene87177:CDS |
| TCACGCAATGGAAGGAGATT+AGG | 0.445908 | 7.4:-65448028 | MS.gene87177:CDS |
| GCAGATGGAACTTTGTCTCT+AGG | 0.487647 | 7.4:-65449662 | MS.gene87177:CDS |
| AGCAGACTGTAGCAGCGAAT+AGG | 0.488975 | 7.4:-65448004 | MS.gene87177:CDS |
| AAGAAGGATGTTGAATGGAA+TGG | 0.494548 | 7.4:-65447611 | MS.gene87177:CDS |
| TTATAAAGAAGGATGTTGAA+TGG | 0.501223 | 7.4:-65447616 | MS.gene87177:CDS |
| AGCAGCATGGTGAAGGCAGA+TGG | 0.518000 | 7.4:-65449677 | None:intergenic |
| TAGATCTGAGATTATAAAGA+AGG | 0.519444 | 7.4:-65447627 | MS.gene87177:CDS |
| TTGTCCACAGCTTCAAGCAA+AGG | 0.523678 | 7.4:+65448363 | None:intergenic |
| AAAACCTTTGCTTGAAGCTG+TGG | 0.526822 | 7.4:-65448367 | MS.gene87177:CDS |
| AAGTGAGGCGGCAGGGCCGT+GGG | 0.549357 | 7.4:-65448243 | MS.gene87177:CDS |
| TATGTCAAGCAGCATGGTGA+AGG | 0.550639 | 7.4:-65449684 | None:intergenic |
| TGAGAAGATTTGGGAAGACA+AGG | 0.554945 | 7.4:-65448091 | MS.gene87177:CDS |
| GCTGTGGACAAGATGAAAGA+AGG | 0.584388 | 7.4:-65448351 | MS.gene87177:intron |
| CGAGACTATCACGCAATGGA+AGG | 0.589824 | 7.4:-65448036 | MS.gene87177:CDS |
| ATAATCTCAGATCTAGTGCA+CGG | 0.625982 | 7.4:+65447635 | None:intergenic |
| GAAACATGCAAATTCTCCCA+CGG | 0.647808 | 7.4:+65448227 | None:intergenic |
| TGCACGAGACTATCACGCAA+TGG | 0.649469 | 7.4:-65448040 | MS.gene87177:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATCATATATAAGTATTA+CGG | - | chr7.4:65448772-65448791 | MS.gene87177:intron | 10.0% |
| !!! | TTTTAATGTTATTACTAATA+TGG | - | chr7.4:65449106-65449125 | MS.gene87177:intron | 10.0% |
| !! | AATGAGTTCAATATTAAAAT+TGG | - | chr7.4:65447794-65447813 | MS.gene87177:intron | 15.0% |
| !! | CAATACATCAAATTAAAAAA+AGG | + | chr7.4:65448738-65448757 | None:intergenic | 15.0% |
| !! | TAATCATATATAAGTATTAC+GGG | - | chr7.4:65448773-65448792 | MS.gene87177:intron | 15.0% |
| !!! | AAATTAAGCTTTTAAATTTG+TGG | - | chr7.4:65447841-65447860 | MS.gene87177:intron | 15.0% |
| !! | ACAAAAATGTGTATAAAGTT+TGG | + | chr7.4:65447657-65447676 | None:intergenic | 20.0% |
| !! | ATCAAAAGTAAAGAAATTAC+AGG | + | chr7.4:65448177-65448196 | None:intergenic | 20.0% |
| !! | TGAAAATATATTACTCATCA+AGG | - | chr7.4:65447977-65447996 | MS.gene87177:intron | 20.0% |
| !! | TGTACTGTATTTATTTGTAA+GGG | - | chr7.4:65448668-65448687 | MS.gene87177:intron | 20.0% |
| !! | TTAGTTTATGTAGAGTAAAA+AGG | + | chr7.4:65448057-65448076 | None:intergenic | 20.0% |
| !! | TTTCTTCTTTATCTCTTAAA+AGG | - | chr7.4:65449521-65449540 | MS.gene87177:intron | 20.0% |
| !!! | ATTTATGGATTAACTAACTT+TGG | + | chr7.4:65447709-65447728 | None:intergenic | 20.0% |
| !!! | TGGTTATATTTTTTTTTCTG+TGG | - | chr7.4:65448862-65448881 | MS.gene87177:intron | 20.0% |
| ! | AATCAGAATTCATACCAAAA+AGG | + | chr7.4:65448840-65448859 | None:intergenic | 25.0% |
| ! | AATTTAGAAAACTGAAGGTT+AGG | + | chr7.4:65447826-65447845 | None:intergenic | 25.0% |
| ! | AGCTTAATTTAGAAAACTGA+AGG | + | chr7.4:65447831-65447850 | None:intergenic | 25.0% |
| ! | AGTCAAAATTCACTGAAAAT+TGG | - | chr7.4:65447740-65447759 | MS.gene87177:intron | 25.0% |
| ! | ATTTAGAAAACTGAAGGTTA+GGG | + | chr7.4:65447825-65447844 | None:intergenic | 25.0% |
| ! | CAAAGTCCAAATCTTATATA+TGG | + | chr7.4:65448307-65448326 | None:intergenic | 25.0% |
| ! | CATATATAAGATTTGGACTT+TGG | - | chr7.4:65448305-65448324 | MS.gene87177:intron | 25.0% |
| ! | CTGTACTGTATTTATTTGTA+AGG | - | chr7.4:65448667-65448686 | MS.gene87177:intron | 25.0% |
| ! | CTTTATATACAAAAAAGTGG+AGG | - | chr7.4:65448221-65448240 | MS.gene87177:CDS | 25.0% |
| ! | CTTTATCTCTTAAAAGGAAA+TGG | - | chr7.4:65449527-65449546 | MS.gene87177:intron | 25.0% |
| ! | GACCTTTATATACAAAAAAG+TGG | - | chr7.4:65448218-65448237 | MS.gene87177:CDS | 25.0% |
| ! | GACTTGAATAACTCAATTTA+TGG | + | chr7.4:65447724-65447743 | None:intergenic | 25.0% |
| ! | TGTTAATCTATGTAGTTGTA+GGG | - | chr7.4:65448492-65448511 | MS.gene87177:intron | 25.0% |
| ! | TTAGCAAAATGCCTTAAAAA+TGG | + | chr7.4:65448550-65448569 | None:intergenic | 25.0% |
| ! | TTATAAAGAAGGATGTTGAA+TGG | - | chr7.4:65449662-65449681 | MS.gene87177:CDS | 25.0% |
| !! | AATATATTTTCATCTCCATG+AGG | + | chr7.4:65447969-65447988 | None:intergenic | 25.0% |
| !! | GTGAATAAACTATTCCTTTT+TGG | - | chr7.4:65448823-65448842 | MS.gene87177:intron | 25.0% |
| !! | TAGATCTGAGATTATAAAGA+AGG | - | chr7.4:65449651-65449670 | MS.gene87177:intron | 25.0% |
| !! | TTAAGCTTTTAAATTTGTGG+AGG | - | chr7.4:65447844-65447863 | MS.gene87177:intron | 25.0% |
| !!! | CTCCACTTTTTTGTATATAA+AGG | + | chr7.4:65448223-65448242 | None:intergenic | 25.0% |
| !!! | TCTTTACTTTTGATACACAA+TGG | - | chr7.4:65448183-65448202 | MS.gene87177:intron | 25.0% |
| AACAATCGACATTTGTTTGT+GGG | - | chr7.4:65448989-65449008 | MS.gene87177:intron | 30.0% | |
| AATATTGAACTCATTCCATC+AGG | + | chr7.4:65447789-65447808 | None:intergenic | 30.0% | |
| ACCACACTTAAATATTTGCT+AGG | + | chr7.4:65448608-65448627 | None:intergenic | 30.0% | |
| ATTCACAAACCCTTACAAAA+GGG | + | chr7.4:65448809-65448828 | None:intergenic | 30.0% | |
| CAGAAACTATAAAAGAACCA+AGG | - | chr7.4:65448348-65448367 | MS.gene87177:intron | 30.0% | |
| CTTATGAATCAAAGAGAAAC+TGG | + | chr7.4:65448140-65448159 | None:intergenic | 30.0% | |
| CTTTGATGGAATGCAAATAT+TGG | + | chr7.4:65449602-65449621 | None:intergenic | 30.0% | |
| GAGAAAAATTACGCATGATA+AGG | + | chr7.4:65448025-65448044 | None:intergenic | 30.0% | |
| GCTCTATTATACAACTTTGA+TGG | + | chr7.4:65449616-65449635 | None:intergenic | 30.0% | |
| GTCCAAATCTTATATATGGA+AGG | + | chr7.4:65448303-65448322 | None:intergenic | 30.0% | |
| GTGTTAATCTATGTAGTTGT+AGG | - | chr7.4:65448491-65448510 | MS.gene87177:intron | 30.0% | |
| GTTCATCAAGTTTCAGTATA+TGG | + | chr7.4:65448085-65448104 | None:intergenic | 30.0% | |
| TAGAGTAAAAAGGCAATTCT+TGG | + | chr7.4:65448047-65448066 | None:intergenic | 30.0% | |
| TATAAAAGAACCAAGGTCAT+TGG | - | chr7.4:65448355-65448374 | MS.gene87177:CDS | 30.0% | |
| TATTCACAAACCCTTACAAA+AGG | + | chr7.4:65448810-65448829 | None:intergenic | 30.0% | |
| TTAAATTCACCTACCATTAG+TGG | + | chr7.4:65449386-65449405 | None:intergenic | 30.0% | |
| ! | AAGAGAAACTGGTTTAATAG+AGG | + | chr7.4:65448129-65448148 | None:intergenic | 30.0% |
| ! | AATTTTACAGTTCAGCAGTA+CGG | - | chr7.4:65449354-65449373 | MS.gene87177:intron | 30.0% |
| ! | TGGATACAAAGTACTATGTT+AGG | + | chr7.4:65447689-65447708 | None:intergenic | 30.0% |
| ! | TTGGTATGAATTCTGATTTG+TGG | - | chr7.4:65448842-65448861 | MS.gene87177:intron | 30.0% |
| !! | TAAGATTTGGACTTTGGTTT+TGG | - | chr7.4:65448311-65448330 | MS.gene87177:intron | 30.0% |
| !!! | AGTGTGGTTAGATTCTTTTT+TGG | - | chr7.4:65448620-65448639 | MS.gene87177:intron | 30.0% |
| !!! | AGTTTTAAACACACTAGACT+AGG | - | chr7.4:65447928-65447947 | MS.gene87177:intron | 30.0% |
| AAAAGTTGCATGGTAGGAAT+TGG | - | chr7.4:65448515-65448534 | MS.gene87177:intron | 35.0% | |
| AAAGTTGCATGGTAGGAATT+GGG | - | chr7.4:65448516-65448535 | MS.gene87177:intron | 35.0% | |
| AAGAAGGATGTTGAATGGAA+TGG | - | chr7.4:65449667-65449686 | MS.gene87177:CDS | 35.0% | |
| AGGCGAAAAAAAAGTAAGTG+AGG | - | chr7.4:65449020-65449039 | MS.gene87177:intron | 35.0% | |
| ATAATCTCAGATCTAGTGCA+CGG | + | chr7.4:65449646-65449665 | None:intergenic | 35.0% | |
| ATTGCAATCAATGTTCCTCA+TGG | - | chr7.4:65447951-65447970 | MS.gene87177:intron | 35.0% | |
| CGAAAAAAAAGTAAGTGAGG+CGG | - | chr7.4:65449023-65449042 | MS.gene87177:intron | 35.0% | |
| GAACAATCGACATTTGTTTG+TGG | - | chr7.4:65448988-65449007 | MS.gene87177:intron | 35.0% | |
| GCCTAGCAAATATTTAAGTG+TGG | - | chr7.4:65448604-65448623 | MS.gene87177:intron | 35.0% | |
| GGCCTTCCATATATAAGATT+TGG | - | chr7.4:65448298-65448317 | MS.gene87177:intron | 35.0% | |
| TGCAGATAACTGGAATTGAA+AGG | - | chr7.4:65448277-65448296 | MS.gene87177:intron | 35.0% | |
| TTTGTTTGTGGGATGTGATA+AGG | - | chr7.4:65449000-65449019 | MS.gene87177:intron | 35.0% | |
| ! | CTTCCCAAATCTTCTCATTT+TGG | + | chr7.4:65449184-65449203 | None:intergenic | 35.0% |
| ! | TACTAATATGGAGCACCATA+AGG | - | chr7.4:65449118-65449137 | MS.gene87177:intron | 35.0% |
| ! | TAGCAAAGTCAATAGCCTTA+TGG | + | chr7.4:65449136-65449155 | None:intergenic | 35.0% |
| ! | TGACTTTGCTATGATGATGT+GGG | - | chr7.4:65449145-65449164 | MS.gene87177:intron | 35.0% |
| ! | TTGACTTTGCTATGATGATG+TGG | - | chr7.4:65449144-65449163 | MS.gene87177:intron | 35.0% |
| !! | GGGAGTTTGTTCCATTTTTA+AGG | - | chr7.4:65448536-65448555 | MS.gene87177:intron | 35.0% |
| !!! | TGTTGTTTTCTCTTTGCTTG+TGG | - | chr7.4:65448397-65448416 | MS.gene87177:intron | 35.0% |
| AAAACCTTTGCTTGAAGCTG+TGG | - | chr7.4:65448911-65448930 | MS.gene87177:intron | 40.0% | |
| AGCGCCAAAATGAGAAGATT+TGG | - | chr7.4:65449177-65449196 | MS.gene87177:intron | 40.0% | |
| AGTTGTAGGGAAAAGTTGCA+TGG | - | chr7.4:65448505-65448524 | MS.gene87177:intron | 40.0% | |
| CAAAGAGAAAACAACAGACC+AGG | + | chr7.4:65448393-65448412 | None:intergenic | 40.0% | |
| GAAACATGCAAATTCTCCCA+CGG | + | chr7.4:65449054-65449073 | None:intergenic | 40.0% | |
| GAACTTGATCTGCAGATAAC+TGG | - | chr7.4:65448267-65448286 | MS.gene87177:intron | 40.0% | |
| GCGCCAAAATGAGAAGATTT+GGG | - | chr7.4:65449178-65449197 | MS.gene87177:intron | 40.0% | |
| TATGTGTATCGGAACTCTAG+AGG | - | chr7.4:65449457-65449476 | MS.gene87177:intron | 40.0% | |
| TGAGAAGATTTGGGAAGACA+AGG | - | chr7.4:65449187-65449206 | MS.gene87177:intron | 40.0% | |
| TTTATGAGTCCTAGACCTGA+TGG | - | chr7.4:65447771-65447790 | MS.gene87177:intron | 40.0% | |
| TTTGTGCTTACTGCTACATC+TGG | - | chr7.4:65449568-65449587 | MS.gene87177:intron | 40.0% | |
| ! | ACCAAAGCTTCCCTTTTGTA+AGG | - | chr7.4:65448796-65448815 | MS.gene87177:intron | 40.0% |
| ! | CCAAAGCTTCCCTTTTGTAA+GGG | - | chr7.4:65448797-65448816 | MS.gene87177:intron | 40.0% |
| ! | GACTTTGCTATGATGATGTG+GGG | - | chr7.4:65449146-65449165 | MS.gene87177:intron | 40.0% |
| ! | GCTTTTAAATTTGTGGAGGC+TGG | - | chr7.4:65447848-65447867 | MS.gene87177:intron | 40.0% |
| !! | GATGGAGTTGGTATGTGTAT+CGG | - | chr7.4:65449446-65449465 | MS.gene87177:intron | 40.0% |
| !!! | TTTTTTTTCTGTGGCTGCAG+TGG | - | chr7.4:65448871-65448890 | MS.gene87177:intron | 40.0% |
| AAAAAAGTAAGTGAGGCGGC+AGG | - | chr7.4:65449027-65449046 | MS.gene87177:intron | 45.0% | |
| AAAAAGTAAGTGAGGCGGCA+GGG | - | chr7.4:65449028-65449047 | MS.gene87177:intron | 45.0% | |
| AACTTTGTCTCTAGGCTTGC+AGG | - | chr7.4:65447624-65447643 | MS.gene87177:CDS | 45.0% | |
| CCCTTACAAAAGGGAAGCTT+TGG | + | chr7.4:65448800-65448819 | None:intergenic | 45.0% | |
| GAACTCATTCCATCAGGTCT+AGG | + | chr7.4:65447783-65447802 | None:intergenic | 45.0% | |
| GAGAAAACAACAGACCAGGT+AGG | + | chr7.4:65448389-65448408 | None:intergenic | 45.0% | |
| GCAGATGGAACTTTGTCTCT+AGG | - | chr7.4:65447616-65447635 | MS.gene87177:CDS | 45.0% | |
| GCTGTGGACAAGATGAAAGA+AGG | - | chr7.4:65448927-65448946 | MS.gene87177:intron | 45.0% | |
| GTAGGGAAAAGTTGCATGGT+AGG | - | chr7.4:65448509-65448528 | MS.gene87177:intron | 45.0% | |
| GTTCTTCCTCCACTTTACTG+AGG | + | chr7.4:65448251-65448270 | None:intergenic | 45.0% | |
| TCACGCAATGGAAGGAGATT+AGG | - | chr7.4:65449250-65449269 | MS.gene87177:intron | 45.0% | |
| TTGTCCACAGCTTCAAGCAA+AGG | + | chr7.4:65448918-65448937 | None:intergenic | 45.0% | |
| TTTACAGTTCAGCAGTACGG+TGG | - | chr7.4:65449357-65449376 | MS.gene87177:intron | 45.0% | |
| AACAGACCAGGTAGGAGTCA+AGG | + | chr7.4:65448381-65448400 | None:intergenic | 50.0% | |
| AGCAGACTGTAGCAGCGAAT+AGG | - | chr7.4:65449274-65449293 | MS.gene87177:intron | 50.0% | |
| AGTACGGTGGATGCCACTAA+TGG | - | chr7.4:65449370-65449389 | MS.gene87177:intron | 50.0% | |
| CGAGACTATCACGCAATGGA+AGG | - | chr7.4:65449242-65449261 | MS.gene87177:intron | 50.0% | |
| GGAAGACCTCAGTAAAGTGG+AGG | - | chr7.4:65448242-65448261 | MS.gene87177:CDS | 50.0% | |
| GGAGGAAGACCTCAGTAAAG+TGG | - | chr7.4:65448239-65448258 | MS.gene87177:CDS | 50.0% | |
| TGCACGAGACTATCACGCAA+TGG | - | chr7.4:65449238-65449257 | MS.gene87177:intron | 50.0% | |
| !! | TTTCAAGAAGAGCGCTGCGA+TGG | - | chr7.4:65449428-65449447 | MS.gene87177:intron | 50.0% |
| CGGTGGATGCCACTAATGGT+AGG | - | chr7.4:65449374-65449393 | MS.gene87177:intron | 55.0% | |
| GGAGTCAAGGCCAATGACCT+TGG | + | chr7.4:65448368-65448387 | None:intergenic | 55.0% | |
| !! | CATTGGCCTTGACTCCTACC+TGG | - | chr7.4:65448372-65448391 | MS.gene87177:CDS | 55.0% |
| TTCTGTGGCTGCAGTGGCTG+TGG | - | chr7.4:65448877-65448896 | MS.gene87177:intron | 60.0% | |
| !! | GAAGAGCGCTGCGATGGAGT+TGG | - | chr7.4:65449434-65449453 | MS.gene87177:intron | 60.0% |
| AAGTGAGGCGGCAGGGCCGT+GGG | - | chr7.4:65449035-65449054 | MS.gene87177:intron | 70.0% | |
| TAAGTGAGGCGGCAGGGCCG+TGG | - | chr7.4:65449034-65449053 | MS.gene87177:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 65447607 | 65449693 | 65447607 | ID=MS.gene87177 |
| chr7.4 | mRNA | 65447607 | 65449693 | 65447607 | ID=MS.gene87177.t1;Parent=MS.gene87177 |
| chr7.4 | exon | 65449655 | 65449693 | 65449655 | ID=MS.gene87177.t1.exon1;Parent=MS.gene87177.t1 |
| chr7.4 | CDS | 65449655 | 65449693 | 65449655 | ID=cds.MS.gene87177.t1;Parent=MS.gene87177.t1 |
| chr7.4 | exon | 65448352 | 65448409 | 65448352 | ID=MS.gene87177.t1.exon2;Parent=MS.gene87177.t1 |
| chr7.4 | CDS | 65448352 | 65448409 | 65448352 | ID=cds.MS.gene87177.t1;Parent=MS.gene87177.t1 |
| chr7.4 | exon | 65448218 | 65448278 | 65448218 | ID=MS.gene87177.t1.exon3;Parent=MS.gene87177.t1 |
| chr7.4 | CDS | 65448218 | 65448278 | 65448218 | ID=cds.MS.gene87177.t1;Parent=MS.gene87177.t1 |
| chr7.4 | exon | 65447983 | 65448124 | 65447983 | ID=MS.gene87177.t1.exon4;Parent=MS.gene87177.t1 |
| chr7.4 | CDS | 65447983 | 65448124 | 65447983 | ID=cds.MS.gene87177.t1;Parent=MS.gene87177.t1 |
| chr7.4 | exon | 65447607 | 65447678 | 65447607 | ID=MS.gene87177.t1.exon5;Parent=MS.gene87177.t1 |
| chr7.4 | CDS | 65447607 | 65447678 | 65447607 | ID=cds.MS.gene87177.t1;Parent=MS.gene87177.t1 |
| Gene Sequence |
| Protein sequence |