AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87177


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCCAAATCTTCTCATTT+TGG 0.143116 7.4:+65448097 None:intergenic
AACTTTGTCTCTAGGCTTGC+AGG 0.274208 7.4:-65449654 MS.gene87177:intron
AGCGCCAAAATGAGAAGATT+TGG 0.293225 7.4:-65448101 MS.gene87177:CDS
TTCTGTGGCTGCAGTGGCTG+TGG 0.414645 7.4:-65448401 MS.gene87177:intron
GCGCCAAAATGAGAAGATTT+GGG 0.435664 7.4:-65448100 MS.gene87177:CDS
TAAGTGAGGCGGCAGGGCCG+TGG 0.445627 7.4:-65448244 MS.gene87177:CDS
TCACGCAATGGAAGGAGATT+AGG 0.445908 7.4:-65448028 MS.gene87177:CDS
GCAGATGGAACTTTGTCTCT+AGG 0.487647 7.4:-65449662 MS.gene87177:CDS
AGCAGACTGTAGCAGCGAAT+AGG 0.488975 7.4:-65448004 MS.gene87177:CDS
AAGAAGGATGTTGAATGGAA+TGG 0.494548 7.4:-65447611 MS.gene87177:CDS
TTATAAAGAAGGATGTTGAA+TGG 0.501223 7.4:-65447616 MS.gene87177:CDS
AGCAGCATGGTGAAGGCAGA+TGG 0.518000 7.4:-65449677 None:intergenic
TAGATCTGAGATTATAAAGA+AGG 0.519444 7.4:-65447627 MS.gene87177:CDS
TTGTCCACAGCTTCAAGCAA+AGG 0.523678 7.4:+65448363 None:intergenic
AAAACCTTTGCTTGAAGCTG+TGG 0.526822 7.4:-65448367 MS.gene87177:CDS
AAGTGAGGCGGCAGGGCCGT+GGG 0.549357 7.4:-65448243 MS.gene87177:CDS
TATGTCAAGCAGCATGGTGA+AGG 0.550639 7.4:-65449684 None:intergenic
TGAGAAGATTTGGGAAGACA+AGG 0.554945 7.4:-65448091 MS.gene87177:CDS
GCTGTGGACAAGATGAAAGA+AGG 0.584388 7.4:-65448351 MS.gene87177:intron
CGAGACTATCACGCAATGGA+AGG 0.589824 7.4:-65448036 MS.gene87177:CDS
ATAATCTCAGATCTAGTGCA+CGG 0.625982 7.4:+65447635 None:intergenic
GAAACATGCAAATTCTCCCA+CGG 0.647808 7.4:+65448227 None:intergenic
TGCACGAGACTATCACGCAA+TGG 0.649469 7.4:-65448040 MS.gene87177:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATCATATATAAGTATTA+CGG - chr7.4:65448772-65448791 MS.gene87177:intron 10.0%
!!! TTTTAATGTTATTACTAATA+TGG - chr7.4:65449106-65449125 MS.gene87177:intron 10.0%
!! AATGAGTTCAATATTAAAAT+TGG - chr7.4:65447794-65447813 MS.gene87177:intron 15.0%
!! CAATACATCAAATTAAAAAA+AGG + chr7.4:65448738-65448757 None:intergenic 15.0%
!! TAATCATATATAAGTATTAC+GGG - chr7.4:65448773-65448792 MS.gene87177:intron 15.0%
!!! AAATTAAGCTTTTAAATTTG+TGG - chr7.4:65447841-65447860 MS.gene87177:intron 15.0%
!! ACAAAAATGTGTATAAAGTT+TGG + chr7.4:65447657-65447676 None:intergenic 20.0%
!! ATCAAAAGTAAAGAAATTAC+AGG + chr7.4:65448177-65448196 None:intergenic 20.0%
!! TGAAAATATATTACTCATCA+AGG - chr7.4:65447977-65447996 MS.gene87177:intron 20.0%
!! TGTACTGTATTTATTTGTAA+GGG - chr7.4:65448668-65448687 MS.gene87177:intron 20.0%
!! TTAGTTTATGTAGAGTAAAA+AGG + chr7.4:65448057-65448076 None:intergenic 20.0%
!! TTTCTTCTTTATCTCTTAAA+AGG - chr7.4:65449521-65449540 MS.gene87177:intron 20.0%
!!! ATTTATGGATTAACTAACTT+TGG + chr7.4:65447709-65447728 None:intergenic 20.0%
!!! TGGTTATATTTTTTTTTCTG+TGG - chr7.4:65448862-65448881 MS.gene87177:intron 20.0%
! AATCAGAATTCATACCAAAA+AGG + chr7.4:65448840-65448859 None:intergenic 25.0%
! AATTTAGAAAACTGAAGGTT+AGG + chr7.4:65447826-65447845 None:intergenic 25.0%
! AGCTTAATTTAGAAAACTGA+AGG + chr7.4:65447831-65447850 None:intergenic 25.0%
! AGTCAAAATTCACTGAAAAT+TGG - chr7.4:65447740-65447759 MS.gene87177:intron 25.0%
! ATTTAGAAAACTGAAGGTTA+GGG + chr7.4:65447825-65447844 None:intergenic 25.0%
! CAAAGTCCAAATCTTATATA+TGG + chr7.4:65448307-65448326 None:intergenic 25.0%
! CATATATAAGATTTGGACTT+TGG - chr7.4:65448305-65448324 MS.gene87177:intron 25.0%
! CTGTACTGTATTTATTTGTA+AGG - chr7.4:65448667-65448686 MS.gene87177:intron 25.0%
! CTTTATATACAAAAAAGTGG+AGG - chr7.4:65448221-65448240 MS.gene87177:CDS 25.0%
! CTTTATCTCTTAAAAGGAAA+TGG - chr7.4:65449527-65449546 MS.gene87177:intron 25.0%
! GACCTTTATATACAAAAAAG+TGG - chr7.4:65448218-65448237 MS.gene87177:CDS 25.0%
! GACTTGAATAACTCAATTTA+TGG + chr7.4:65447724-65447743 None:intergenic 25.0%
! TGTTAATCTATGTAGTTGTA+GGG - chr7.4:65448492-65448511 MS.gene87177:intron 25.0%
! TTAGCAAAATGCCTTAAAAA+TGG + chr7.4:65448550-65448569 None:intergenic 25.0%
! TTATAAAGAAGGATGTTGAA+TGG - chr7.4:65449662-65449681 MS.gene87177:CDS 25.0%
!! AATATATTTTCATCTCCATG+AGG + chr7.4:65447969-65447988 None:intergenic 25.0%
!! GTGAATAAACTATTCCTTTT+TGG - chr7.4:65448823-65448842 MS.gene87177:intron 25.0%
!! TAGATCTGAGATTATAAAGA+AGG - chr7.4:65449651-65449670 MS.gene87177:intron 25.0%
!! TTAAGCTTTTAAATTTGTGG+AGG - chr7.4:65447844-65447863 MS.gene87177:intron 25.0%
!!! CTCCACTTTTTTGTATATAA+AGG + chr7.4:65448223-65448242 None:intergenic 25.0%
!!! TCTTTACTTTTGATACACAA+TGG - chr7.4:65448183-65448202 MS.gene87177:intron 25.0%
AACAATCGACATTTGTTTGT+GGG - chr7.4:65448989-65449008 MS.gene87177:intron 30.0%
AATATTGAACTCATTCCATC+AGG + chr7.4:65447789-65447808 None:intergenic 30.0%
ACCACACTTAAATATTTGCT+AGG + chr7.4:65448608-65448627 None:intergenic 30.0%
ATTCACAAACCCTTACAAAA+GGG + chr7.4:65448809-65448828 None:intergenic 30.0%
CAGAAACTATAAAAGAACCA+AGG - chr7.4:65448348-65448367 MS.gene87177:intron 30.0%
CTTATGAATCAAAGAGAAAC+TGG + chr7.4:65448140-65448159 None:intergenic 30.0%
CTTTGATGGAATGCAAATAT+TGG + chr7.4:65449602-65449621 None:intergenic 30.0%
GAGAAAAATTACGCATGATA+AGG + chr7.4:65448025-65448044 None:intergenic 30.0%
GCTCTATTATACAACTTTGA+TGG + chr7.4:65449616-65449635 None:intergenic 30.0%
GTCCAAATCTTATATATGGA+AGG + chr7.4:65448303-65448322 None:intergenic 30.0%
GTGTTAATCTATGTAGTTGT+AGG - chr7.4:65448491-65448510 MS.gene87177:intron 30.0%
GTTCATCAAGTTTCAGTATA+TGG + chr7.4:65448085-65448104 None:intergenic 30.0%
TAGAGTAAAAAGGCAATTCT+TGG + chr7.4:65448047-65448066 None:intergenic 30.0%
TATAAAAGAACCAAGGTCAT+TGG - chr7.4:65448355-65448374 MS.gene87177:CDS 30.0%
TATTCACAAACCCTTACAAA+AGG + chr7.4:65448810-65448829 None:intergenic 30.0%
TTAAATTCACCTACCATTAG+TGG + chr7.4:65449386-65449405 None:intergenic 30.0%
! AAGAGAAACTGGTTTAATAG+AGG + chr7.4:65448129-65448148 None:intergenic 30.0%
! AATTTTACAGTTCAGCAGTA+CGG - chr7.4:65449354-65449373 MS.gene87177:intron 30.0%
! TGGATACAAAGTACTATGTT+AGG + chr7.4:65447689-65447708 None:intergenic 30.0%
! TTGGTATGAATTCTGATTTG+TGG - chr7.4:65448842-65448861 MS.gene87177:intron 30.0%
!! TAAGATTTGGACTTTGGTTT+TGG - chr7.4:65448311-65448330 MS.gene87177:intron 30.0%
!!! AGTGTGGTTAGATTCTTTTT+TGG - chr7.4:65448620-65448639 MS.gene87177:intron 30.0%
!!! AGTTTTAAACACACTAGACT+AGG - chr7.4:65447928-65447947 MS.gene87177:intron 30.0%
AAAAGTTGCATGGTAGGAAT+TGG - chr7.4:65448515-65448534 MS.gene87177:intron 35.0%
AAAGTTGCATGGTAGGAATT+GGG - chr7.4:65448516-65448535 MS.gene87177:intron 35.0%
AAGAAGGATGTTGAATGGAA+TGG - chr7.4:65449667-65449686 MS.gene87177:CDS 35.0%
AGGCGAAAAAAAAGTAAGTG+AGG - chr7.4:65449020-65449039 MS.gene87177:intron 35.0%
ATAATCTCAGATCTAGTGCA+CGG + chr7.4:65449646-65449665 None:intergenic 35.0%
ATTGCAATCAATGTTCCTCA+TGG - chr7.4:65447951-65447970 MS.gene87177:intron 35.0%
CGAAAAAAAAGTAAGTGAGG+CGG - chr7.4:65449023-65449042 MS.gene87177:intron 35.0%
GAACAATCGACATTTGTTTG+TGG - chr7.4:65448988-65449007 MS.gene87177:intron 35.0%
GCCTAGCAAATATTTAAGTG+TGG - chr7.4:65448604-65448623 MS.gene87177:intron 35.0%
GGCCTTCCATATATAAGATT+TGG - chr7.4:65448298-65448317 MS.gene87177:intron 35.0%
TGCAGATAACTGGAATTGAA+AGG - chr7.4:65448277-65448296 MS.gene87177:intron 35.0%
TTTGTTTGTGGGATGTGATA+AGG - chr7.4:65449000-65449019 MS.gene87177:intron 35.0%
! CTTCCCAAATCTTCTCATTT+TGG + chr7.4:65449184-65449203 None:intergenic 35.0%
! TACTAATATGGAGCACCATA+AGG - chr7.4:65449118-65449137 MS.gene87177:intron 35.0%
! TAGCAAAGTCAATAGCCTTA+TGG + chr7.4:65449136-65449155 None:intergenic 35.0%
! TGACTTTGCTATGATGATGT+GGG - chr7.4:65449145-65449164 MS.gene87177:intron 35.0%
! TTGACTTTGCTATGATGATG+TGG - chr7.4:65449144-65449163 MS.gene87177:intron 35.0%
!! GGGAGTTTGTTCCATTTTTA+AGG - chr7.4:65448536-65448555 MS.gene87177:intron 35.0%
!!! TGTTGTTTTCTCTTTGCTTG+TGG - chr7.4:65448397-65448416 MS.gene87177:intron 35.0%
AAAACCTTTGCTTGAAGCTG+TGG - chr7.4:65448911-65448930 MS.gene87177:intron 40.0%
AGCGCCAAAATGAGAAGATT+TGG - chr7.4:65449177-65449196 MS.gene87177:intron 40.0%
AGTTGTAGGGAAAAGTTGCA+TGG - chr7.4:65448505-65448524 MS.gene87177:intron 40.0%
CAAAGAGAAAACAACAGACC+AGG + chr7.4:65448393-65448412 None:intergenic 40.0%
GAAACATGCAAATTCTCCCA+CGG + chr7.4:65449054-65449073 None:intergenic 40.0%
GAACTTGATCTGCAGATAAC+TGG - chr7.4:65448267-65448286 MS.gene87177:intron 40.0%
GCGCCAAAATGAGAAGATTT+GGG - chr7.4:65449178-65449197 MS.gene87177:intron 40.0%
TATGTGTATCGGAACTCTAG+AGG - chr7.4:65449457-65449476 MS.gene87177:intron 40.0%
TGAGAAGATTTGGGAAGACA+AGG - chr7.4:65449187-65449206 MS.gene87177:intron 40.0%
TTTATGAGTCCTAGACCTGA+TGG - chr7.4:65447771-65447790 MS.gene87177:intron 40.0%
TTTGTGCTTACTGCTACATC+TGG - chr7.4:65449568-65449587 MS.gene87177:intron 40.0%
! ACCAAAGCTTCCCTTTTGTA+AGG - chr7.4:65448796-65448815 MS.gene87177:intron 40.0%
! CCAAAGCTTCCCTTTTGTAA+GGG - chr7.4:65448797-65448816 MS.gene87177:intron 40.0%
! GACTTTGCTATGATGATGTG+GGG - chr7.4:65449146-65449165 MS.gene87177:intron 40.0%
! GCTTTTAAATTTGTGGAGGC+TGG - chr7.4:65447848-65447867 MS.gene87177:intron 40.0%
!! GATGGAGTTGGTATGTGTAT+CGG - chr7.4:65449446-65449465 MS.gene87177:intron 40.0%
!!! TTTTTTTTCTGTGGCTGCAG+TGG - chr7.4:65448871-65448890 MS.gene87177:intron 40.0%
AAAAAAGTAAGTGAGGCGGC+AGG - chr7.4:65449027-65449046 MS.gene87177:intron 45.0%
AAAAAGTAAGTGAGGCGGCA+GGG - chr7.4:65449028-65449047 MS.gene87177:intron 45.0%
AACTTTGTCTCTAGGCTTGC+AGG - chr7.4:65447624-65447643 MS.gene87177:CDS 45.0%
CCCTTACAAAAGGGAAGCTT+TGG + chr7.4:65448800-65448819 None:intergenic 45.0%
GAACTCATTCCATCAGGTCT+AGG + chr7.4:65447783-65447802 None:intergenic 45.0%
GAGAAAACAACAGACCAGGT+AGG + chr7.4:65448389-65448408 None:intergenic 45.0%
GCAGATGGAACTTTGTCTCT+AGG - chr7.4:65447616-65447635 MS.gene87177:CDS 45.0%
GCTGTGGACAAGATGAAAGA+AGG - chr7.4:65448927-65448946 MS.gene87177:intron 45.0%
GTAGGGAAAAGTTGCATGGT+AGG - chr7.4:65448509-65448528 MS.gene87177:intron 45.0%
GTTCTTCCTCCACTTTACTG+AGG + chr7.4:65448251-65448270 None:intergenic 45.0%
TCACGCAATGGAAGGAGATT+AGG - chr7.4:65449250-65449269 MS.gene87177:intron 45.0%
TTGTCCACAGCTTCAAGCAA+AGG + chr7.4:65448918-65448937 None:intergenic 45.0%
TTTACAGTTCAGCAGTACGG+TGG - chr7.4:65449357-65449376 MS.gene87177:intron 45.0%
AACAGACCAGGTAGGAGTCA+AGG + chr7.4:65448381-65448400 None:intergenic 50.0%
AGCAGACTGTAGCAGCGAAT+AGG - chr7.4:65449274-65449293 MS.gene87177:intron 50.0%
AGTACGGTGGATGCCACTAA+TGG - chr7.4:65449370-65449389 MS.gene87177:intron 50.0%
CGAGACTATCACGCAATGGA+AGG - chr7.4:65449242-65449261 MS.gene87177:intron 50.0%
GGAAGACCTCAGTAAAGTGG+AGG - chr7.4:65448242-65448261 MS.gene87177:CDS 50.0%
GGAGGAAGACCTCAGTAAAG+TGG - chr7.4:65448239-65448258 MS.gene87177:CDS 50.0%
TGCACGAGACTATCACGCAA+TGG - chr7.4:65449238-65449257 MS.gene87177:intron 50.0%
!! TTTCAAGAAGAGCGCTGCGA+TGG - chr7.4:65449428-65449447 MS.gene87177:intron 50.0%
CGGTGGATGCCACTAATGGT+AGG - chr7.4:65449374-65449393 MS.gene87177:intron 55.0%
GGAGTCAAGGCCAATGACCT+TGG + chr7.4:65448368-65448387 None:intergenic 55.0%
!! CATTGGCCTTGACTCCTACC+TGG - chr7.4:65448372-65448391 MS.gene87177:CDS 55.0%
TTCTGTGGCTGCAGTGGCTG+TGG - chr7.4:65448877-65448896 MS.gene87177:intron 60.0%
!! GAAGAGCGCTGCGATGGAGT+TGG - chr7.4:65449434-65449453 MS.gene87177:intron 60.0%
AAGTGAGGCGGCAGGGCCGT+GGG - chr7.4:65449035-65449054 MS.gene87177:intron 70.0%
TAAGTGAGGCGGCAGGGCCG+TGG - chr7.4:65449034-65449053 MS.gene87177:intron 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 65447607 65449693 65447607 ID=MS.gene87177
chr7.4 mRNA 65447607 65449693 65447607 ID=MS.gene87177.t1;Parent=MS.gene87177
chr7.4 exon 65449655 65449693 65449655 ID=MS.gene87177.t1.exon1;Parent=MS.gene87177.t1
chr7.4 CDS 65449655 65449693 65449655 ID=cds.MS.gene87177.t1;Parent=MS.gene87177.t1
chr7.4 exon 65448352 65448409 65448352 ID=MS.gene87177.t1.exon2;Parent=MS.gene87177.t1
chr7.4 CDS 65448352 65448409 65448352 ID=cds.MS.gene87177.t1;Parent=MS.gene87177.t1
chr7.4 exon 65448218 65448278 65448218 ID=MS.gene87177.t1.exon3;Parent=MS.gene87177.t1
chr7.4 CDS 65448218 65448278 65448218 ID=cds.MS.gene87177.t1;Parent=MS.gene87177.t1
chr7.4 exon 65447983 65448124 65447983 ID=MS.gene87177.t1.exon4;Parent=MS.gene87177.t1
chr7.4 CDS 65447983 65448124 65447983 ID=cds.MS.gene87177.t1;Parent=MS.gene87177.t1
chr7.4 exon 65447607 65447678 65447607 ID=MS.gene87177.t1.exon5;Parent=MS.gene87177.t1
chr7.4 CDS 65447607 65447678 65447607 ID=cds.MS.gene87177.t1;Parent=MS.gene87177.t1
Gene Sequence

>MS.gene87177

ATGGTGAAGGCAGATGGAACTTTGTCTCTAGGCTTGCAGGTATACAACCAAACTTTATACACATTTTTGTATTTTAATTCCTAACATAGTACTTTGTATCCAAAGTTAGTTAATCCATAAATTGAGTTATTCAAGTCAAAATTCACTGAAAATTGGCATGTATATTTATGAGTCCTAGACCTGATGGAATGAGTTCAATATTAAAATTGGTGCAACCCTAACCTTCAGTTTTCTAAATTAAGCTTTTAAATTTGTGGAGGCTGGCTACTTTGAAAACACTTTTGTCATTTCTTTCTAAATTTTGTATCAAGAGTCGATATGAGTTTTAAACACACTAGACTAGGATTGCAATCAATGTTCCTCATGGAGATGAAAATATATTACTCATCAAGGAACTGATTTCAACATTCATAATCCTTATCATGCGTAATTTTTCTCCAAGAATTGCCTTTTTACTCTACATAAACTAAGAGCACCATATACTGAAACTTGATGAACAATTGTAATGTAACAGAATTTCCTCTATTAAACCAGTTTCTCTTTGATTCATAAGATTTCGATTCATTGCCTGTAATTTCTTTACTTTTGATACACAATGGAACAATCTGAATGACCTTTATATACAAAAAAGTGGAGGAAGACCTCAGTAAAGTGGAGGAAGAACTTGATCTGCAGATAACTGGAATTGAAAGGCCTTCCATATATAAGATTTGGACTTTGGTTTTGGATACTTCAAGATAGCAGAAACTATAAAAGAACCAAGGTCATTGGCCTTGACTCCTACCTGGTCTGTTGTTTTCTCTTTGCTTGTGGATCACTCAATTAATCGTTTAAGCAATGTAAGTATTGCTTGAAATCTTATTGTTGAGTTTTGTTAGTGTTTTGTGTTAATCTATGTAGTTGTAGGGAAAAGTTGCATGGTAGGAATTGGGAGTTTGTTCCATTTTTAAGGCATTTTGCTAACTTAAGAATTAAGACTTAGCATAAAAAGTATTTTGCCTAGCAAATATTTAAGTGTGGTTAGATTCTTTTTTGGATTTTTTCTCTATGACAAATTTATCTGTACTGTATTTATTTGTAAGGGAAGAAGTATCTGTTAATGCAATTGCAGCTATATATATAAATCTACCTTTTTTTAATTTGATGTATTGAGCTTGATTCTATCTTAATCATATATAAGTATTACGGGACCAAAGCTTCCCTTTTGTAAGGGTTTGTGAATAAACTATTCCTTTTTGGTATGAATTCTGATTTGTGGTTATATTTTTTTTTCTGTGGCTGCAGTGGCTGTGGAAAACTAATAGAAAACCTTTGCTTGAAGCTGTGGACAAGATGAAAGAAGGTACGCACGCAAATCATTTTGTGCTGAAACAAACTATGAGAACAATCGACATTTGTTTGTGGGATGTGATAAGGCGAAAAAAAAGTAAGTGAGGCGGCAGGGCCGTGGGAGAATTTGCATGTTTCAATACAAAAGTAACAGATTTTGCAAATTTGCTTTTTAATGTTATTACTAATATGGAGCACCATAAGGCTATTGACTTTGCTATGATGATGTGGGGTATATAGAAGCGCCAAAATGAGAAGATTTGGGAAGACAAGGAGAATAATGTTCAAGTATCAATTAAGATTGCACGAGACTATCACGCAATGGAAGGAGATTAGGAAGCAGACTGTAGCAGCGAATAGGAATAGCAGTACAGCAGCAGAAGTGCAAAATAGTTGCAGTTTAGCAGCTGCGCAAAACAATTTTACAGTTCAGCAGTACGGTGGATGCCACTAATGGTAGGTGAATTTAAATGTAACATTGACGCAGCACTGTTTCAAGAAGAGCGCTGCGATGGAGTTGGTATGTGTATCGGAACTCTAGAGGACATTATATGACAATTTAATTTGTTCTATTTTAGAGCTTTCTTTCTTCTTTATCTCTTAAAAGGAAATGGTTATCTGCATTTTTTTTTTTTGTGCTTACTGCTACATCTGGCATGATAGCCAATATTTGCATTCCATCAAAGTTGTATAATAGAGCTTTTGCTCCGTGCACTAGATCTGAGATTATAAAGAAGGATGTTGAATGGAATGGATAG

Protein sequence

>MS.gene87177.t1

MVKADGTLSLGLQWLWKTNRKPLLEAVDKMKEGEKKVSEAAGPWENLHVSIQKSAKMRRFGKTRRIMFKYQLRLHETITQWKEIRKQTVAANRNSSTAAELYNRAFAPCTRSEIIKKDVEWNG