Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87481.t1 | XP_013455017.1 | 100 | 102 | 0 | 0 | 1 | 102 | 20 | 121 | 2.70E-50 | 207.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87481.t1 | O80934 | 77.5 | 102 | 23 | 0 | 1 | 102 | 90 | 191 | 3.6e-43 | 175.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87481.t1 | B7FIE9 | 100.0 | 102 | 0 | 0 | 1 | 102 | 20 | 121 | 2.0e-50 | 207.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049599 | MS.gene87481 | -0.834925 | 2.26E-56 | -1.69E-46 |
| MS.gene049758 | MS.gene87481 | 0.838202 | 3.31E-57 | -1.69E-46 |
| MS.gene05119 | MS.gene87481 | -0.812131 | 4.95E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene87481 | 0.812486 | 4.14E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene87481 | 0.82894 | 6.80E-55 | -1.69E-46 |
| MS.gene05984 | MS.gene87481 | 0.805647 | 1.21E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene87481 | 0.813174 | 2.92E-51 | -1.69E-46 |
| MS.gene059899 | MS.gene87481 | 0.824263 | 8.87E-54 | -1.69E-46 |
| MS.gene060084 | MS.gene87481 | 0.803065 | 4.16E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCTGTACCTTGAATTGC+AGG | 0.352388 | 4.1:-74859150 | None:intergenic |
| AAACTCAGGCCTTCCACCTT+TGG | 0.364997 | 4.1:-74859226 | None:intergenic |
| TATGATAGGTTGACTGGATT+GGG | 0.379614 | 4.1:+74859369 | MS.gene87481:intron |
| CCAGTATATTGCAAGAGGTT+TGG | 0.393691 | 4.1:+74859044 | MS.gene87481:CDS |
| AACAGCATTAGCACCTGTTC+GGG | 0.426709 | 4.1:-74859270 | None:intergenic |
| CCAAACCTCTTGCAATATAC+TGG | 0.433246 | 4.1:-74859044 | None:intergenic |
| CAAACCCAGGTTTCATTTGT+GGG | 0.437050 | 4.1:-74859197 | None:intergenic |
| AATTGCAGGAACAATACTTT+CGG | 0.439185 | 4.1:-74859136 | None:intergenic |
| TAACAGCATTAGCACCTGTT+CGG | 0.440664 | 4.1:-74859271 | None:intergenic |
| ATATGATAGGTTGACTGGAT+TGG | 0.441585 | 4.1:+74859368 | MS.gene87481:intron |
| AGTATTGTTCCTGCAATTCA+AGG | 0.441646 | 4.1:+74859141 | MS.gene87481:CDS |
| TCAAACCCAGGTTTCATTTG+TGG | 0.465991 | 4.1:-74859198 | None:intergenic |
| TCAGGCCTTCCACCTTTGGT+TGG | 0.499708 | 4.1:-74859222 | None:intergenic |
| TGATGGTGCATATCCCGAAC+AGG | 0.546256 | 4.1:+74859257 | MS.gene87481:CDS |
| GCTGTCCCACAAATGAAACC+TGG | 0.549601 | 4.1:+74859192 | MS.gene87481:CDS |
| CGAGACCAGTATATTGCAAG+AGG | 0.560446 | 4.1:+74859039 | MS.gene87481:CDS |
| CCTTTGGTTGGATCAAACCC+AGG | 0.573204 | 4.1:-74859210 | None:intergenic |
| CGGCATTTCTGATATCCCCA+AGG | 0.590513 | 4.1:-74859116 | None:intergenic |
| GGGTTTGATCCAACCAAAGG+TGG | 0.593347 | 4.1:+74859213 | MS.gene87481:CDS |
| TTGATCCAACCAAAGGTGGA+AGG | 0.601871 | 4.1:+74859217 | MS.gene87481:CDS |
| CCATCATCAAAATAAAACTC+AGG | 0.606577 | 4.1:-74859240 | None:intergenic |
| CTGTCCCACAAATGAAACCT+GGG | 0.620890 | 4.1:+74859193 | MS.gene87481:CDS |
| CCTGGGTTTGATCCAACCAA+AGG | 0.647766 | 4.1:+74859210 | MS.gene87481:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATATTCTTAGCATGCAT+AGG | - | chr4.1:74859304-74859323 | None:intergenic | 25.0% |
| ! | AACCTATCATATGAAAAAGT+AGG | - | chr4.1:74859360-74859379 | None:intergenic | 25.0% |
| ! | GAAGAAAGCAAACAAAAAAT+TGG | + | chr4.1:74859075-74859094 | MS.gene87481:CDS | 25.0% |
| AATTGCAGGAACAATACTTT+CGG | - | chr4.1:74859139-74859158 | None:intergenic | 30.0% | |
| CAGAAAAATCAAATAGATGC+TGG | + | chr4.1:74859392-74859411 | MS.gene87481:CDS | 30.0% | |
| CCATCATCAAAATAAAACTC+AGG | - | chr4.1:74859243-74859262 | None:intergenic | 30.0% | |
| GAAAGCAAACAAAAAATTGG+CGG | + | chr4.1:74859078-74859097 | MS.gene87481:CDS | 30.0% | |
| !! | TTTTCATATGATAGGTTGAC+TGG | + | chr4.1:74859363-74859382 | MS.gene87481:intron | 30.0% |
| !!! | CCTGAGTTTTATTTTGATGA+TGG | + | chr4.1:74859240-74859259 | MS.gene87481:CDS | 30.0% |
| !!! | GACCTACTTTTTCATATGAT+AGG | + | chr4.1:74859355-74859374 | MS.gene87481:intron | 30.0% |
| AGTATTGTTCCTGCAATTCA+AGG | + | chr4.1:74859141-74859160 | MS.gene87481:CDS | 35.0% | |
| ! | ATATGATAGGTTGACTGGAT+TGG | + | chr4.1:74859368-74859387 | MS.gene87481:intron | 35.0% |
| ! | GTGCAGATGATATTTTCCTT+GGG | + | chr4.1:74859100-74859119 | MS.gene87481:CDS | 35.0% |
| ! | TATGATAGGTTGACTGGATT+GGG | + | chr4.1:74859369-74859388 | MS.gene87481:intron | 35.0% |
| ! | TGCAGATGATATTTTCCTTG+GGG | + | chr4.1:74859101-74859120 | MS.gene87481:CDS | 35.0% |
| CAAACCCAGGTTTCATTTGT+GGG | - | chr4.1:74859200-74859219 | None:intergenic | 40.0% | |
| CCAAACCTCTTGCAATATAC+TGG | - | chr4.1:74859047-74859066 | None:intergenic | 40.0% | |
| CCAGTATATTGCAAGAGGTT+TGG | + | chr4.1:74859044-74859063 | MS.gene87481:CDS | 40.0% | |
| TAACAGCATTAGCACCTGTT+CGG | - | chr4.1:74859274-74859293 | None:intergenic | 40.0% | |
| TCAAACCCAGGTTTCATTTG+TGG | - | chr4.1:74859201-74859220 | None:intergenic | 40.0% | |
| ! | GGTGCAGATGATATTTTCCT+TGG | + | chr4.1:74859099-74859118 | MS.gene87481:CDS | 40.0% |
| AACAGCATTAGCACCTGTTC+GGG | - | chr4.1:74859273-74859292 | None:intergenic | 45.0% | |
| CGAGACCAGTATATTGCAAG+AGG | + | chr4.1:74859039-74859058 | MS.gene87481:CDS | 45.0% | |
| CTGTCCCACAAATGAAACCT+GGG | + | chr4.1:74859193-74859212 | MS.gene87481:CDS | 45.0% | |
| GCATCTGTACCTTGAATTGC+AGG | - | chr4.1:74859153-74859172 | None:intergenic | 45.0% | |
| TTGATCCAACCAAAGGTGGA+AGG | + | chr4.1:74859217-74859236 | MS.gene87481:CDS | 45.0% | |
| AAACTCAGGCCTTCCACCTT+TGG | - | chr4.1:74859229-74859248 | None:intergenic | 50.0% | |
| CCTGGGTTTGATCCAACCAA+AGG | + | chr4.1:74859210-74859229 | MS.gene87481:CDS | 50.0% | |
| CCTTTGGTTGGATCAAACCC+AGG | - | chr4.1:74859213-74859232 | None:intergenic | 50.0% | |
| CGGCATTTCTGATATCCCCA+AGG | - | chr4.1:74859119-74859138 | None:intergenic | 50.0% | |
| GCTGTCCCACAAATGAAACC+TGG | + | chr4.1:74859192-74859211 | MS.gene87481:CDS | 50.0% | |
| GGGTTTGATCCAACCAAAGG+TGG | + | chr4.1:74859213-74859232 | MS.gene87481:CDS | 50.0% | |
| ! | TGATGGTGCATATCCCGAAC+AGG | + | chr4.1:74859257-74859276 | MS.gene87481:CDS | 50.0% |
| TCAGGCCTTCCACCTTTGGT+TGG | - | chr4.1:74859225-74859244 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 74859007 | 74859413 | 74859007 | ID=MS.gene87481 |
| chr4.1 | mRNA | 74859007 | 74859413 | 74859007 | ID=MS.gene87481.t1;Parent=MS.gene87481 |
| chr4.1 | exon | 74859007 | 74859278 | 74859007 | ID=MS.gene87481.t1.exon1;Parent=MS.gene87481.t1 |
| chr4.1 | CDS | 74859007 | 74859278 | 74859007 | ID=cds.MS.gene87481.t1;Parent=MS.gene87481.t1 |
| chr4.1 | exon | 74859377 | 74859413 | 74859377 | ID=MS.gene87481.t1.exon2;Parent=MS.gene87481.t1 |
| chr4.1 | CDS | 74859377 | 74859413 | 74859377 | ID=cds.MS.gene87481.t1;Parent=MS.gene87481.t1 |
| Gene Sequence |
| Protein sequence |
