AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87552

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87552.t1 MTR_4g023030 97.432 623 16 0 1 623 1 623 0.0 1252
MS.gene87552.t1 MTR_3g026650 68.860 623 134 9 1 623 1 563 0.0 800
MS.gene87552.t1 MTR_3g026650 67.093 547 120 9 1 547 1 487 0.0 665
MS.gene87552.t1 MTR_3g026650 63.341 461 109 9 1 461 1 401 2.00e-177 509
MS.gene87552.t1 MTR_4g125460 36.977 430 251 8 198 616 58 478 3.82e-88 283
MS.gene87552.t1 MTR_4g125460 36.977 430 251 8 198 616 197 617 1.02e-86 283
MS.gene87552.t1 MTR_5g064620 34.286 490 267 12 164 616 40 511 9.75e-80 262
MS.gene87552.t1 MTR_3g096020 36.508 441 243 11 199 616 160 586 1.42e-78 261
MS.gene87552.t1 MTR_5g099010 36.427 431 255 9 196 619 84 502 3.15e-77 256
MS.gene87552.t1 MTR_4g100810 39.231 390 217 9 236 616 111 489 7.32e-77 254
MS.gene87552.t1 MTR_5g081690 35.189 449 270 10 179 616 72 510 1.01e-76 254
MS.gene87552.t1 MTR_3g115220 35.177 452 256 8 191 619 58 495 2.37e-75 251
MS.gene87552.t1 MTR_3g099480 36.501 463 241 13 184 616 98 537 3.45e-75 250
MS.gene87552.t1 MTR_4g082310 36.409 401 236 8 236 622 100 495 8.45e-75 248
MS.gene87552.t1 MTR_5g012770 37.907 430 234 11 202 622 95 500 1.22e-74 248
MS.gene87552.t1 MTR_4g019210 33.945 436 268 8 196 620 199 625 5.93e-73 247
MS.gene87552.t1 MTR_5g032100 34.725 455 246 10 201 616 59 501 6.40e-73 243
MS.gene87552.t1 MTR_4g084570 34.014 441 261 10 201 622 124 553 6.81e-72 242
MS.gene87552.t1 MTR_5g042200 35.802 405 235 9 236 623 88 484 2.01e-70 236
MS.gene87552.t1 MTR_6g016015 37.333 375 219 8 236 599 118 487 2.57e-70 236
MS.gene87552.t1 MTR_4g110030 37.634 372 220 6 236 598 119 487 3.35e-70 236
MS.gene87552.t1 MTR_1g031590 33.548 465 274 11 175 617 96 547 4.47e-70 237
MS.gene87552.t1 MTR_3g100310 33.698 457 250 10 200 616 72 515 6.78e-70 235
MS.gene87552.t1 MTR_3g086570 33.108 444 260 12 199 616 115 547 3.41e-69 234
MS.gene87552.t1 MTR_2g059350 36.685 368 219 6 236 593 118 481 1.48e-68 232
MS.gene87552.t1 MTR_5g059260 32.222 450 275 9 191 616 116 559 1.90e-65 225
MS.gene87552.t1 MTR_3g005340 33.586 396 247 6 236 616 150 544 3.49e-64 222
MS.gene87552.t1 MTR_1g102030 30.159 441 277 11 198 619 57 485 1.16e-57 202
MS.gene87552.t1 MTR_4g023050 96.250 80 3 0 544 623 29 108 7.44e-51 171
MS.gene87552.t1 MTR_2g016470 29.325 474 264 16 199 616 156 614 1.49e-49 182
MS.gene87552.t1 MTR_4g132590 31.130 469 261 17 199 616 35 492 1.03e-47 175
MS.gene87552.t1 MTR_4g132590 31.130 469 261 17 199 616 134 591 7.26e-47 174
MS.gene87552.t1 MTR_3g086570 31.077 325 190 10 199 498 115 430 1.51e-43 162
MS.gene87552.t1 MTR_1g101990 31.690 284 182 7 341 619 5 281 2.17e-42 155
MS.gene87552.t1 MTR_4g010990 28.530 347 204 10 236 564 78 398 1.34e-30 125
MS.gene87552.t1 MTR_7g017330 29.655 290 189 6 236 510 101 390 4.32e-30 126
MS.gene87552.t1 MTR_4g010990 27.844 334 197 10 249 564 2 309 6.79e-27 112
MS.gene87552.t1 MTR_3g092590 27.273 330 222 7 196 510 56 382 1.73e-26 114
MS.gene87552.t1 MTR_3g092590 27.273 330 222 7 196 510 56 382 2.09e-26 114
MS.gene87552.t1 MTR_1g088900 56.322 87 8 1 321 407 38 94 1.10e-22 98.2
MS.gene87552.t1 MTR_2g038320 24.603 378 220 13 213 569 77 410 5.88e-21 96.3
MS.gene87552.t1 MTR_4g008200 24.474 380 220 12 194 561 14 338 5.93e-21 95.5
MS.gene87552.t1 MTR_4g008200 24.093 386 214 12 194 561 65 389 1.27e-20 95.1
MS.gene87552.t1 MTR_1g007390 27.547 265 177 6 261 510 124 388 1.48e-20 96.7
MS.gene87552.t1 MTR_7g064210 31.100 209 129 4 339 535 19 224 1.86e-20 94.7
MS.gene87552.t1 MTR_1g101985 39.091 110 63 1 198 307 57 162 4.46e-17 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87552.t1 AT2G44500 63.072 612 157 9 11 618 17 563 0.0 748
MS.gene87552.t1 AT3G07900 59.481 617 192 13 10 621 14 577 0.0 681
MS.gene87552.t1 AT5G63390 60.563 426 167 1 199 623 130 555 0.0 553
MS.gene87552.t1 AT2G44500 56.264 455 130 9 11 461 17 406 9.36e-163 472
MS.gene87552.t1 AT5G35570 37.104 442 246 11 198 622 220 646 7.81e-82 271
MS.gene87552.t1 AT1G76270 35.909 440 249 10 194 619 69 489 3.99e-79 261
MS.gene87552.t1 AT1G20550 35.431 429 266 8 194 619 72 492 2.12e-78 259
MS.gene87552.t1 AT4G24530 35.590 458 242 11 199 616 72 516 1.74e-77 256
MS.gene87552.t1 AT5G64600 36.199 442 245 11 199 617 88 515 2.19e-76 253
MS.gene87552.t1 AT3G54100 33.879 428 267 7 202 620 195 615 1.61e-75 254
MS.gene87552.t1 AT1G22460 36.073 438 243 10 202 616 134 557 6.44e-75 250
MS.gene87552.t1 AT5G01100 34.101 434 266 8 202 623 190 615 1.37e-73 248
MS.gene87552.t1 AT5G65470 33.913 460 251 10 199 617 56 503 2.85e-73 244
MS.gene87552.t1 AT2G03280 38.070 373 215 7 237 598 92 459 6.56e-73 243
MS.gene87552.t1 AT2G37980 33.103 435 261 9 202 620 199 619 9.49e-73 246
MS.gene87552.t1 AT4G38390 38.462 377 203 7 235 596 125 487 1.40e-72 243
MS.gene87552.t1 AT1G14970 33.562 438 255 9 201 616 9 432 2.39e-70 234
MS.gene87552.t1 AT1G14020 35.930 398 234 8 237 619 95 486 1.71e-69 234
MS.gene87552.t1 AT5G15740 36.193 373 224 6 236 598 109 477 6.67e-69 233
MS.gene87552.t1 AT1G14970 32.692 468 278 10 172 616 99 552 1.62e-68 233
MS.gene87552.t1 AT1G14970 33.981 412 244 7 226 616 2 406 9.22e-68 227
MS.gene87552.t1 AT2G03280 35.500 400 215 8 237 598 92 486 3.53e-67 228
MS.gene87552.t1 AT4G16650 40.360 389 216 11 236 617 125 504 5.72e-67 228
MS.gene87552.t1 AT4G16650 40.360 389 216 11 236 617 125 504 5.72e-67 228
MS.gene87552.t1 AT3G26370 33.920 398 243 8 236 616 161 555 3.34e-66 227
MS.gene87552.t1 AT3G02250 34.828 379 221 6 236 598 109 477 2.58e-65 223
MS.gene87552.t1 AT1G04910 31.542 428 266 11 202 616 61 474 1.04e-64 222
MS.gene87552.t1 AT1G35510 32.346 439 261 9 202 618 134 558 7.88e-64 221
MS.gene87552.t1 AT2G01480 31.991 447 268 9 191 615 118 550 2.57e-63 219
MS.gene87552.t1 AT1G38065 32.850 414 254 10 224 616 18 428 1.89e-62 214
MS.gene87552.t1 AT1G38065 33.085 402 246 9 236 616 39 438 2.31e-62 214
MS.gene87552.t1 AT1G38131 32.850 414 254 10 224 616 147 557 1.96e-61 214
MS.gene87552.t1 AT2G01480 33.333 405 239 7 191 575 118 511 2.10e-61 213
MS.gene87552.t1 AT1G62330 29.193 483 259 15 198 618 190 651 2.87e-54 196
MS.gene87552.t1 AT1G11990 30.737 475 288 16 167 617 132 589 3.86e-53 192
MS.gene87552.t1 AT1G11990 29.106 481 267 15 198 617 144 611 3.03e-47 176
MS.gene87552.t1 AT1G29200 32.397 463 236 18 212 616 50 493 1.86e-46 171
MS.gene87552.t1 AT1G29200 32.397 463 236 18 212 616 50 493 1.86e-46 171
MS.gene87552.t1 AT1G29200 32.397 463 236 18 212 616 166 609 5.01e-46 172
MS.gene87552.t1 AT1G29200 33.102 432 223 16 238 616 1 419 6.86e-45 165
MS.gene87552.t1 AT3G30300 32.759 290 180 6 236 510 103 392 6.93e-35 140
MS.gene87552.t1 AT3G30300 32.759 290 180 6 236 510 103 392 6.93e-35 140
MS.gene87552.t1 AT1G52630 24.480 433 280 13 195 616 39 435 1.98e-29 122
MS.gene87552.t1 AT3G26370 32.967 182 108 4 236 404 161 341 9.21e-29 119
MS.gene87552.t1 AT3G03810 30.375 293 183 7 236 510 92 381 5.72e-27 116
MS.gene87552.t1 AT3G03810 30.375 293 183 7 236 510 92 381 5.72e-27 116
MS.gene87552.t1 AT1G52630 24.802 379 241 12 249 616 2 347 4.33e-25 107

Find 142 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTATGGTGGTTGTTTC+TGG 0.073685 4.1:-75359438 MS.gene87552:CDS
ACCAAACCCATTTGAGATTA+AGG 0.186427 4.1:+75359845 None:intergenic
GTTGTTATTGCTAGGATTCT+TGG 0.223923 4.1:-75359378 MS.gene87552:CDS
ATGACATTAACTTGCAAAAT+AGG 0.227744 4.1:+75359339 None:intergenic
GCCATCACATGGAGGAAATA+TGG 0.245491 4.1:-75357452 MS.gene87552:CDS
TCTTCACCACCTTAACAAAA+TGG 0.248210 4.1:-75359995 MS.gene87552:CDS
TATAGGTTGCTTAAAGCTTT+AGG 0.261962 4.1:-75357679 MS.gene87552:intron
GACCAGCCATCTTTCTTTCA+TGG 0.284935 4.1:+75358091 None:intergenic
GTTTCCACATCTTTATAATA+AGG 0.294769 4.1:-75357575 MS.gene87552:CDS
TTTGCAAGTTAATGTCATTT+GGG 0.301248 4.1:-75359334 MS.gene87552:CDS
AAAAGATGCAAGAATTTATT+GGG 0.322642 4.1:-75357650 MS.gene87552:CDS
AAATATGGGCCATGCGCTTC+AGG 0.329180 4.1:-75357437 MS.gene87552:intron
GAAAAGCCCTTAATCTCAAA+TGG 0.333986 4.1:-75359852 MS.gene87552:CDS
CCAAACCCATTTGAGATTAA+GGG 0.341142 4.1:+75359846 None:intergenic
CAAAACCCTTCAATCTCAAA+TGG 0.341182 4.1:-75359735 MS.gene87552:CDS
TCAAATTCACTTCCTTTGAT+TGG 0.342422 4.1:-75359642 MS.gene87552:CDS
CCCTTAATCTCAAATGGGTT+TGG 0.349127 4.1:-75359846 MS.gene87552:CDS
CTCAGAAACTATGTAATCTA+TGG 0.350072 4.1:+75357492 None:intergenic
TGGAAGCAACCAGATGGGTT+AGG 0.362833 4.1:-75359534 MS.gene87552:CDS
GAGGGAAGGATTCAGAAAAT+AGG 0.364333 4.1:+75356891 None:intergenic
TAGGATTCTTGGTGCTGCTT+TGG 0.371467 4.1:-75359367 MS.gene87552:CDS
ATGCTGTTAAACTCTGCTTC+AGG 0.374694 4.1:+75356868 None:intergenic
TTGAATAGAAGAAGATGAAA+TGG 0.388256 4.1:+75360076 None:intergenic
TGGTTCAAAATCTGAACTTA+AGG 0.388422 4.1:-75359826 MS.gene87552:CDS
GTTAGGGTATAAGCCTTGTT+TGG 0.390330 4.1:-75359517 MS.gene87552:CDS
GTGAGGACTGGTTGTCTACC+TGG 0.392388 4.1:-75358198 MS.gene87552:CDS
GAATTGCATCAGAATTCATT+AGG 0.395674 4.1:-75356838 MS.gene87552:CDS
GGATTGTGAAGAATAGAAAA+AGG 0.396440 4.1:-75359461 MS.gene87552:CDS
TGTGGAAACTCATGGATTAA+TGG 0.400222 4.1:+75357589 None:intergenic
GAGTTGAAAAGAGTGAGTTT+TGG 0.403781 4.1:-75359554 MS.gene87552:CDS
TTGTTTCCAAGTTCTTGAAC+TGG 0.412948 4.1:+75358291 None:intergenic
ACCTTGCTAGTCCTGAGTTC+CGG 0.418631 4.1:+75356811 None:intergenic
GCGAAACCAGTTCAAGAACT+TGG 0.420208 4.1:-75358297 MS.gene87552:CDS
TCCGTTCGCGCTATCTCAAA+AGG 0.424692 4.1:-75358765 MS.gene87552:CDS
AGGGAAGGATTCAGAAAATA+GGG 0.430895 4.1:+75356892 None:intergenic
TGCGAAGAAAGCTTCTCTAA+TGG 0.432693 4.1:-75357518 MS.gene87552:CDS
TGACTTGGAGGATGAAGAAA+AGG 0.432865 4.1:-75359583 MS.gene87552:CDS
CGAAACCACCCGCACATCAT+TGG 0.433723 4.1:+75358861 None:intergenic
GGAAGCAACCAGATGGGTTA+GGG 0.436626 4.1:-75359533 MS.gene87552:CDS
TCAAACCCATTTGAGATTGA+AGG 0.439951 4.1:+75359729 None:intergenic
GGTGGTGAAGAAGAAGTTAG+AGG 0.440438 4.1:+75360007 None:intergenic
ATGCAAGAATTTATTGGGCC+GGG 0.443277 4.1:-75357645 MS.gene87552:CDS
GTATTTCTTGTGTCCTGCAT+AGG 0.450341 4.1:+75356942 None:intergenic
AGAGAAGCTTTCTTCGCAAA+TGG 0.451160 4.1:+75357523 None:intergenic
GGCTTATACCCTAACCCATC+TGG 0.457469 4.1:+75359525 None:intergenic
GGAGGATGAAGAAAAGGGTG+AGG 0.460916 4.1:-75359577 MS.gene87552:CDS
TTTGTCATCAAGTGTGTTGA+TGG 0.461208 4.1:+75358832 None:intergenic
GGTTCCTAGTGGAGTTGACT+TGG 0.467672 4.1:-75359598 MS.gene87552:CDS
TATAAAGATGTGGAAACTCA+TGG 0.469538 4.1:+75357581 None:intergenic
AAGGAAGATCTTGCATTGCC+TGG 0.471250 4.1:-75357556 MS.gene87552:CDS
TCCACCCAATGGTTGTCCCC+CGG 0.472197 4.1:+75357627 None:intergenic
AAAACCCTTCAATCTCAAAT+GGG 0.474207 4.1:-75359734 MS.gene87552:CDS
TGCTGTTAAACTCTGCTTCA+GGG 0.476049 4.1:+75356869 None:intergenic
CCATCACATGGAGGAAATAT+GGG 0.480168 4.1:-75357451 MS.gene87552:CDS
TAGCGCGAACGGATCCAACT+TGG 0.483932 4.1:+75358775 None:intergenic
AAAAGCCCTTAATCTCAAAT+GGG 0.485351 4.1:-75359851 MS.gene87552:CDS
CCTTGCTCTTCATCTAAGAA+TGG 0.490124 4.1:-75358235 MS.gene87552:CDS
CTTATGGTGGTTGTTTCTGG+AGG 0.490684 4.1:-75359435 MS.gene87552:CDS
GATGCAAGAATTTATTGGGC+CGG 0.492621 4.1:-75357646 MS.gene87552:CDS
AAGGATGTGTGGGTGAGGAC+TGG 0.493609 4.1:-75358210 MS.gene87552:CDS
CAAACCCATTTGAGATTGAA+GGG 0.494557 4.1:+75359730 None:intergenic
GATCTTCCTTTCAAGAGCTC+CGG 0.494862 4.1:+75358126 None:intergenic
CTCTTCATGTCACTCCAAGT+TGG 0.504620 4.1:-75358789 MS.gene87552:CDS
TGCAAGAATTTATTGGGCCG+GGG 0.505342 4.1:-75357644 MS.gene87552:CDS
GCAAATGGTTCTAGTTCTCC+AGG 0.505643 4.1:+75357538 None:intergenic
AACTCAGGACTAGCAAGGTC+GGG 0.506943 4.1:-75356807 MS.gene87552:CDS
AGCCTTGTTTGGATTTCAGT+AGG 0.508226 4.1:-75359506 MS.gene87552:CDS
TTGCACATGCACTCTGGGAC+AGG 0.510427 4.1:+75356766 None:intergenic
CCTTTGCAGTTCAACAGAGA+AGG 0.513180 4.1:-75358504 MS.gene87552:intron
AACTCTGCTTCAGGGAGGGA+AGG 0.515606 4.1:+75356877 None:intergenic
TCTTTGTCCCTTGACTGCCA+TGG 0.516067 4.1:-75358070 MS.gene87552:CDS
GAGTCGTTGCACATGCACTC+TGG 0.519336 4.1:+75356760 None:intergenic
TCCTAGTGGAGTTGACTTGG+AGG 0.524121 4.1:-75359595 MS.gene87552:CDS
ATTATAGAAGAGAAAGTGAT+GGG 0.526617 4.1:-75359482 MS.gene87552:CDS
TCTTCATCTAAGAATGGAAA+AGG 0.529212 4.1:-75358229 MS.gene87552:CDS
ATGTCATTTGGGGAGATGAA+AGG 0.532142 4.1:-75359323 MS.gene87552:intron
GCCTTGTTTGGATTTCAGTA+GGG 0.532530 4.1:-75359505 MS.gene87552:CDS
GGTAACATCCATGGCAGTCA+AGG 0.535475 4.1:+75358062 None:intergenic
AGTCGTTGCACATGCACTCT+GGG 0.536700 4.1:+75356761 None:intergenic
TCTTAGATGAAGAGCAAGGT+AGG 0.537764 4.1:+75358239 None:intergenic
AACATTACATTACCAATCAA+AGG 0.538428 4.1:+75359630 None:intergenic
GCTATCGAAGGATCTTCCTT+CGG 0.538608 4.1:-75358451 MS.gene87552:CDS
GAATGAGAGGAGAGAACGAC+CGG 0.540471 4.1:-75358145 MS.gene87552:CDS
TATTTACCTGGTAACATCCA+TGG 0.544732 4.1:+75358053 None:intergenic
TCAGAATTCATTAGGACAGC+CGG 0.547453 4.1:-75356830 MS.gene87552:CDS
TGGAAAAGGATGTGTGGGTG+AGG 0.548660 4.1:-75358215 MS.gene87552:CDS
TAATGTAATGTTGCAAGCAA+AGG 0.548895 4.1:-75359619 MS.gene87552:CDS
CATTAGGACAGCCGGAACTC+AGG 0.555136 4.1:-75356822 MS.gene87552:CDS
TTGATGCTGTTGTTATTGCT+AGG 0.555554 4.1:-75359386 MS.gene87552:CDS
ACGAGATTGTAAAGAATGAG+AGG 0.556539 4.1:-75358158 MS.gene87552:CDS
GAACGACCGGAGCTCTTGAA+AGG 0.557470 4.1:-75358132 MS.gene87552:CDS
GATTATAGAAGAGAAAGTGA+TGG 0.557924 4.1:-75359483 MS.gene87552:CDS
GAGGATGAAGAAAAGGGTGA+GGG 0.561229 4.1:-75359576 MS.gene87552:CDS
TTTACAATCTCGTCAAACTC+AGG 0.561693 4.1:+75358168 None:intergenic
TACCATGAAAGAAAGATGGC+TGG 0.562902 4.1:-75358093 MS.gene87552:CDS
CCCATATTTCCTCCATGTGA+TGG 0.566940 4.1:+75357451 None:intergenic
ATGGAATTGATTATTTGTGA+AGG 0.568945 4.1:+75360095 None:intergenic
TTATGGTGGTTGTTTCTGGA+GGG 0.569275 4.1:-75359434 MS.gene87552:CDS
AGAGGAGGACTTCCCTCCAC+AGG 0.571987 4.1:+75358808 None:intergenic
CAACTTGGAGTGACATGAAG+AGG 0.572279 4.1:+75358790 None:intergenic
TTCAACATTTGTAGCACATG+GGG 0.574252 4.1:+75359872 None:intergenic
CAGGGTCACAGAGCCTATGC+AGG 0.575525 4.1:-75356955 MS.gene87552:CDS
AAAAGAAGAACAATCTTGAA+GGG 0.576810 4.1:+75359967 None:intergenic
CCGTTCGCGCTATCTCAAAA+GGG 0.577744 4.1:-75358764 MS.gene87552:intron
GACTTACCATGAAAGAAAGA+TGG 0.579868 4.1:-75358097 MS.gene87552:CDS
TCTTCAGAAGCTTCGATGCA+AGG 0.581403 4.1:-75358427 MS.gene87552:intron
GAACTCAGGACTAGCAAGGT+CGG 0.582708 4.1:-75356808 MS.gene87552:CDS
TTTGATTCAAAATCTGAACT+TGG 0.584212 4.1:-75359711 MS.gene87552:CDS
TGTTCTTGCCAATGATGTGC+GGG 0.584306 4.1:-75358869 MS.gene87552:CDS
GACTTGGAGGATGAAGAAAA+GGG 0.591227 4.1:-75359582 MS.gene87552:CDS
TCTTCCTTATTATAAAGATG+TGG 0.591443 4.1:+75357571 None:intergenic
TGACTGCCATGGATGTTACC+AGG 0.594053 4.1:-75358059 MS.gene87552:intron
TTTCAACATTTGTAGCACAT+GGG 0.594469 4.1:+75359871 None:intergenic
GCAAGAATTTATTGGGCCGG+GGG 0.602690 4.1:-75357643 MS.gene87552:CDS
CGAAGCTTCTGAAGATCCGA+AGG 0.604591 4.1:+75358435 None:intergenic
TTGCAAGTTAATGTCATTTG+GGG 0.605474 4.1:-75359333 MS.gene87552:CDS
GTTAAACTCTGCTTCAGGGA+GGG 0.608437 4.1:+75356873 None:intergenic
TCAGGACTAGCAAGGTCGGG+AGG 0.608590 4.1:-75356804 MS.gene87552:CDS
TCCTCCAAGTCAACTCCACT+AGG 0.609217 4.1:+75359594 None:intergenic
CTTGATGACAAAGCCTGTGG+AGG 0.609422 4.1:-75358821 MS.gene87552:CDS
CTTAGATGAAGAGCAAGGTA+GGG 0.612955 4.1:+75358240 None:intergenic
CCATTCTTAGATGAAGAGCA+AGG 0.618532 4.1:+75358235 None:intergenic
CTTGGATTCAAGGCTATCGA+AGG 0.620323 4.1:-75358463 MS.gene87552:CDS
GTAACATCCATGGCAGTCAA+GGG 0.621567 4.1:+75358063 None:intergenic
TTGATGACAAAGCCTGTGGA+GGG 0.624103 4.1:-75358820 MS.gene87552:CDS
GCTGAGAGAATGCAAAGCAA+AGG 0.624230 4.1:-75358264 MS.gene87552:CDS
AAGAATGGAAAAGGATGTGT+GGG 0.627477 4.1:-75358220 MS.gene87552:CDS
CAGGACTAGCAAGGTCGGGA+GGG 0.628531 4.1:-75356803 MS.gene87552:CDS
TGTTTCAATGAATAAAGATG+AGG 0.632505 4.1:-75359685 MS.gene87552:CDS
TTTGGTAAAATCTGAACTTG+GGG 0.642353 4.1:-75359763 MS.gene87552:CDS
CTTGGAGTGACATGAAGAGG+AGG 0.650220 4.1:+75358793 None:intergenic
TCCCTACTGAAATCCAAACA+AGG 0.655365 4.1:+75359504 None:intergenic
TTGCAAGCAAAGGTTCCTAG+TGG 0.655758 4.1:-75359609 MS.gene87552:CDS
TAAGAATGGAAAAGGATGTG+TGG 0.664578 4.1:-75358221 MS.gene87552:CDS
GCCGGAACTCAGGACTAGCA+AGG 0.665088 4.1:-75356812 MS.gene87552:CDS
CTGGAGGGATGAATCAGCAG+AGG 0.666148 4.1:-75359419 MS.gene87552:CDS
ATAGTCATACCTGAAGCGCA+TGG 0.668438 4.1:+75357428 None:intergenic
ACACTTGATGACAAAGCCTG+TGG 0.670917 4.1:-75358824 MS.gene87552:CDS
TCAAACTCAGGACTTAGACC+AGG 0.670921 4.1:+75358180 None:intergenic
ATGTTCTTGCCAATGATGTG+CGG 0.671242 4.1:-75358870 MS.gene87552:CDS
TCTTGCCAATGATGTGCGGG+TGG 0.696204 4.1:-75358866 MS.gene87552:CDS
TGTTAAACTCTGCTTCAGGG+AGG 0.699216 4.1:+75356872 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAAATGAGATTTTTAA+TGG - chr4.1:75357095-75357114 MS.gene87552:intron 10.0%
!!! TTATTATATTTTTTTCTCAT+TGG - chr4.1:75357937-75357956 MS.gene87552:intron 10.0%
!! TTATTAAATATGAAGACTAA+AGG + chr4.1:75359667-75359686 None:intergenic 15.0%
!!! ATTAAATTCTACTTTTGTAT+AGG - chr4.1:75358856-75358875 MS.gene87552:CDS 15.0%
!!! TTTATTTGTTTTGGATTTAT+AGG - chr4.1:75359191-75359210 MS.gene87552:intron 15.0%
!! AAAAAGATGCAAGAATTTAT+TGG - chr4.1:75359236-75359255 MS.gene87552:intron 20.0%
!! AAAAGATGCAAGAATTTATT+GGG - chr4.1:75359237-75359256 MS.gene87552:intron 20.0%
!! AATCAATGAAGATTTATGTA+AGG - chr4.1:75357641-75357660 MS.gene87552:CDS 20.0%
!! AATTGCATGATTACAATTTA+TGG + chr4.1:75359791-75359810 None:intergenic 20.0%
!! AGTAGAATTTAATATTTACC+TGG + chr4.1:75358849-75358868 None:intergenic 20.0%
!! ATATTTAGCTGTTACAAATT+TGG - chr4.1:75357751-75357770 MS.gene87552:intron 20.0%
!! GCTAAATATATATCCAAATT+TGG + chr4.1:75357740-75357759 None:intergenic 20.0%
!! TTACACTTATGAAACTATTT+GGG - chr4.1:75358919-75358938 MS.gene87552:intron 20.0%
!! TTCTGTGGAAATAAAAATTT+GGG - chr4.1:75358316-75358335 MS.gene87552:CDS 20.0%
!! TTTACACTTATGAAACTATT+TGG - chr4.1:75358918-75358937 MS.gene87552:intron 20.0%
!!! AGAGTTTTGTTTATTTGTTT+TGG - chr4.1:75359182-75359201 MS.gene87552:intron 20.0%
!!! AGGATACAATCAATTATTTT+AGG - chr4.1:75359720-75359739 MS.gene87552:CDS 20.0%
!!! AGGTTTAGTTATTACAATTT+TGG - chr4.1:75357661-75357680 MS.gene87552:CDS 20.0%
!!! ATTTTTGTTGAAGATTTCTT+TGG + chr4.1:75356858-75356877 None:intergenic 20.0%
!!! GGATACAATCAATTATTTTA+GGG - chr4.1:75359721-75359740 MS.gene87552:CDS 20.0%
!!! TATTTTTTTCTCATTGGTTT+TGG - chr4.1:75357943-75357962 MS.gene87552:intron 20.0%
!!! TGTGCTTTTAGTAATTAAAA+AGG - chr4.1:75359693-75359712 MS.gene87552:CDS 20.0%
!!! TTATGGAGAAAATATGTTTT+TGG + chr4.1:75359774-75359793 None:intergenic 20.0%
! AAAAAGAAGAACAATCTTGA+AGG + chr4.1:75356924-75356943 None:intergenic 25.0%
! AAAAGAAGAACAATCTTGAA+GGG + chr4.1:75356923-75356942 None:intergenic 25.0%
! AACATTACATTACCAATCAA+AGG + chr4.1:75357260-75357279 None:intergenic 25.0%
! AATTAAGATAGTCACATTGA+TGG - chr4.1:75359807-75359826 MS.gene87552:CDS 25.0%
! ATACAGTATATATGAATGCA+TGG + chr4.1:75359579-75359598 None:intergenic 25.0%
! ATCTTCATTTGTGTAACAAT+CGG - chr4.1:75358503-75358522 MS.gene87552:intron 25.0%
! ATGGAATTGATTATTTGTGA+AGG + chr4.1:75356795-75356814 None:intergenic 25.0%
! ATTATAGAAGAGAAAGTGAT+GGG - chr4.1:75357405-75357424 MS.gene87552:intron 25.0%
! ATTGTTTGAAAGACCAAATT+TGG - chr4.1:75357724-75357743 MS.gene87552:intron 25.0%
! CTTCTGTGGAAATAAAAATT+TGG - chr4.1:75358315-75358334 MS.gene87552:CDS 25.0%
! GTAATCATGCTTACATATAT+TGG - chr4.1:75359553-75359572 MS.gene87552:CDS 25.0%
! GTTTCCACATCTTTATAATA+AGG - chr4.1:75359312-75359331 MS.gene87552:intron 25.0%
! TCTTCCTTATTATAAAGATG+TGG + chr4.1:75359319-75359338 None:intergenic 25.0%
! TGTTTCAATGAATAAAGATG+AGG - chr4.1:75357202-75357221 MS.gene87552:intron 25.0%
! TTAGTAATTAAAAAGGACTG+AGG - chr4.1:75359700-75359719 MS.gene87552:CDS 25.0%
! TTGAATAGAAGAAGATGAAA+TGG + chr4.1:75356814-75356833 None:intergenic 25.0%
! TTTACTATTCTTCTTGTTCA+TGG - chr4.1:75357592-75357611 MS.gene87552:CDS 25.0%
! TTTGATTCAAAATCTGAACT+TGG - chr4.1:75357176-75357195 MS.gene87552:intron 25.0%
!! AAGGTGAGTACAAATTTTAT+AGG + chr4.1:75359648-75359667 None:intergenic 25.0%
!! AATCTTTTTGTTCTTGATAC+TGG - chr4.1:75357843-75357862 MS.gene87552:intron 25.0%
!! AGGTGAGTACAAATTTTATA+GGG + chr4.1:75359647-75359666 None:intergenic 25.0%
!! TTTGCAAGTTAATGTCATTT+GGG - chr4.1:75357553-75357572 MS.gene87552:CDS 25.0%
!! TTTTGCAAGTTAATGTCATT+TGG - chr4.1:75357552-75357571 MS.gene87552:CDS 25.0%
!! TTTTGGTAAAATCTGAACTT+GGG - chr4.1:75357123-75357142 MS.gene87552:intron 25.0%
!!! ATGACATTAACTTGCAAAAT+AGG + chr4.1:75357551-75357570 None:intergenic 25.0%
!!! GAATAGAAAAAGGTATCTTA+TGG - chr4.1:75357436-75357455 MS.gene87552:intron 25.0%
!!! GATTTTTAATGGTGATGTTT+TGG - chr4.1:75357106-75357125 MS.gene87552:intron 25.0%
!!! GTTTTTGCTTTGTTTATGAT+TGG - chr4.1:75356963-75356982 MS.gene87552:intron 25.0%
!!! TTTTTCACACTCTTATTTCT+TGG - chr4.1:75356939-75356958 MS.gene87552:CDS 25.0%
AAAACCCTTCAATCTCAAAT+GGG - chr4.1:75357153-75357172 MS.gene87552:intron 30.0%
AAAAGCCCTTAATCTCAAAT+GGG - chr4.1:75357036-75357055 MS.gene87552:intron 30.0%
AATAAGCTGGAAACAGTTTA+TGG + chr4.1:75358974-75358993 None:intergenic 30.0%
ATCTTGAGAAAGAACAACTT+TGG - chr4.1:75357779-75357798 MS.gene87552:intron 30.0%
CAAAAACTTAGCAACAACTT+AGG + chr4.1:75358198-75358217 None:intergenic 30.0%
CTCAGAAACTATGTAATCTA+TGG + chr4.1:75359398-75359417 None:intergenic 30.0%
GAATTGCATCAGAATTCATT+AGG - chr4.1:75360049-75360068 MS.gene87552:CDS 30.0%
GATTATAGAAGAGAAAGTGA+TGG - chr4.1:75357404-75357423 MS.gene87552:intron 30.0%
GGATTGTGAAGAATAGAAAA+AGG - chr4.1:75357426-75357445 MS.gene87552:intron 30.0%
TAAAAGCAACCTGAAAACAT+AGG + chr4.1:75358556-75358575 None:intergenic 30.0%
TAATGTAATGTTGCAAGCAA+AGG - chr4.1:75357268-75357287 MS.gene87552:intron 30.0%
TATAAAGATGTGGAAACTCA+TGG + chr4.1:75359309-75359328 None:intergenic 30.0%
TATAGGTTGCTTAAAGCTTT+AGG - chr4.1:75359208-75359227 MS.gene87552:intron 30.0%
TCAAATTCACTTCCTTTGAT+TGG - chr4.1:75357245-75357264 MS.gene87552:intron 30.0%
TCTTCATCTAAGAATGGAAA+AGG - chr4.1:75358658-75358677 MS.gene87552:intron 30.0%
TCTTCTAGCATTCACAAATA+AGG + chr4.1:75357903-75357922 None:intergenic 30.0%
TGGTTCAAAATCTGAACTTA+AGG - chr4.1:75357061-75357080 MS.gene87552:intron 30.0%
TGTTTATCCAAAGATCCTAT+AGG - chr4.1:75359122-75359141 MS.gene87552:intron 30.0%
TTATAGGGACTAAAGTTGAA+AGG + chr4.1:75359632-75359651 None:intergenic 30.0%
TTTCAACATTTGTAGCACAT+GGG + chr4.1:75357019-75357038 None:intergenic 30.0%
TTTGGTAAAATCTGAACTTG+GGG - chr4.1:75357124-75357143 MS.gene87552:intron 30.0%
! ACTGATTTCATAAGCTATCT+TGG + chr4.1:75359009-75359028 None:intergenic 30.0%
! AGGGTTTTTCCATTTTGTTA+AGG + chr4.1:75356904-75356923 None:intergenic 30.0%
! CTCTTATTCGAGTTTATTAC+TGG - chr4.1:75358893-75358912 MS.gene87552:CDS 30.0%
! GTTTTTCCATTTTGTTAAGG+TGG + chr4.1:75356901-75356920 None:intergenic 30.0%
! TTGATGAGTCGTGATTTTAT+AGG - chr4.1:75359747-75359766 MS.gene87552:CDS 30.0%
! TTGCAAGTTAATGTCATTTG+GGG - chr4.1:75357554-75357573 MS.gene87552:CDS 30.0%
! TTTTCAACATTTGTAGCACA+TGG + chr4.1:75357020-75357039 None:intergenic 30.0%
!! ACTCATGTTTTTGCATCTTT+AGG - chr4.1:75358165-75358184 MS.gene87552:CDS 30.0%
!! ATGCATTTGTCTTTTGTTTG+GGG + chr4.1:75359975-75359994 None:intergenic 30.0%
!! GATGCATTTGTCTTTTGTTT+GGG + chr4.1:75359976-75359995 None:intergenic 30.0%
!! GTTTTGGTAAAATCTGAACT+TGG - chr4.1:75357122-75357141 MS.gene87552:intron 30.0%
!! TAGAAAAAGGTATCTTATGG+TGG - chr4.1:75357439-75357458 MS.gene87552:CDS 30.0%
!!! AAAGATGAGGTTTTGAATGA+TGG - chr4.1:75357215-75357234 MS.gene87552:intron 30.0%
AACAAGATCAATCTATGCCT+AGG + chr4.1:75357699-75357718 None:intergenic 35.0%
AAGAATGGAAAAGGATGTGT+GGG - chr4.1:75358667-75358686 MS.gene87552:intron 35.0%
ACCTAAATGTTATTCCTTCC+AGG - chr4.1:75359913-75359932 MS.gene87552:CDS 35.0%
ACGAGATTGTAAAGAATGAG+AGG - chr4.1:75358729-75358748 MS.gene87552:intron 35.0%
AGGGAAGGATTCAGAAAATA+GGG + chr4.1:75359998-75360017 None:intergenic 35.0%
CAAAACCCTTCAATCTCAAA+TGG - chr4.1:75357152-75357171 MS.gene87552:intron 35.0%
CCTAAATGTTATTCCTTCCA+GGG - chr4.1:75359914-75359933 MS.gene87552:CDS 35.0%
GAAAAGCCCTTAATCTCAAA+TGG - chr4.1:75357035-75357054 MS.gene87552:intron 35.0%
GACTTACCATGAAAGAAAGA+TGG - chr4.1:75358790-75358809 MS.gene87552:CDS 35.0%
TAAGAATGGAAAAGGATGTG+TGG - chr4.1:75358666-75358685 MS.gene87552:intron 35.0%
TATTTACCTGGTAACATCCA+TGG + chr4.1:75358837-75358856 None:intergenic 35.0%
TCTTCACCACCTTAACAAAA+TGG - chr4.1:75356892-75356911 MS.gene87552:CDS 35.0%
TTCAACATTTGTAGCACATG+GGG + chr4.1:75357018-75357037 None:intergenic 35.0%
TTGTTTCCAAGTTCTTGAAC+TGG + chr4.1:75358599-75358618 None:intergenic 35.0%
TTTACAATCTCGTCAAACTC+AGG + chr4.1:75358722-75358741 None:intergenic 35.0%
TTTGGACTTAATCTAAGCCT+AGG - chr4.1:75357679-75357698 MS.gene87552:intron 35.0%
TTTGTCATCAAGTGTGTTGA+TGG + chr4.1:75358058-75358077 None:intergenic 35.0%
! AATGAAAGCTTTGACTTCTG+TGG - chr4.1:75358301-75358320 MS.gene87552:CDS 35.0%
! ACCAAACCCATTTGAGATTA+AGG + chr4.1:75357045-75357064 None:intergenic 35.0%
! CAAACCCATTTGAGATTGAA+GGG + chr4.1:75357160-75357179 None:intergenic 35.0%
! CCAAACCCATTTGAGATTAA+GGG + chr4.1:75357044-75357063 None:intergenic 35.0%
! CTTTTGTATAGGAGTCTGTT+TGG - chr4.1:75358867-75358886 MS.gene87552:CDS 35.0%
! GAGTTGAAAAGAGTGAGTTT+TGG - chr4.1:75357333-75357352 MS.gene87552:intron 35.0%
! GGATGCATTTGTCTTTTGTT+TGG + chr4.1:75359977-75359996 None:intergenic 35.0%
! GTTGTTATTGCTAGGATTCT+TGG - chr4.1:75357509-75357528 MS.gene87552:CDS 35.0%
! TATCTTATGGTGGTTGTTTC+TGG - chr4.1:75357449-75357468 MS.gene87552:CDS 35.0%
! TCAAACCCATTTGAGATTGA+AGG + chr4.1:75357161-75357180 None:intergenic 35.0%
! TGTGGAAACTCATGGATTAA+TGG + chr4.1:75359301-75359320 None:intergenic 35.0%
! TTGATGCTGTTGTTATTGCT+AGG - chr4.1:75357501-75357520 MS.gene87552:CDS 35.0%
!!! GCACATGGGGTTTTAAAAAA+CGG + chr4.1:75357005-75357024 None:intergenic 35.0%
AGAGAAGCTTTCTTCGCAAA+TGG + chr4.1:75359367-75359386 None:intergenic 40.0%
AGTGAAGCCTATAGGATCTT+TGG + chr4.1:75359132-75359151 None:intergenic 40.0%
ATGCAAGAATTTATTGGGCC+GGG - chr4.1:75359242-75359261 MS.gene87552:intron 40.0%
ATGCTGTTAAACTCTGCTTC+AGG + chr4.1:75360022-75360041 None:intergenic 40.0%
ATGTTCTTGCCAATGATGTG+CGG - chr4.1:75358017-75358036 MS.gene87552:intron 40.0%
CACTGAAAAGTGAAGCCTAT+AGG + chr4.1:75359140-75359159 None:intergenic 40.0%
CCATCACATGGAGGAAATAT+GGG - chr4.1:75359436-75359455 MS.gene87552:CDS 40.0%
CCATTCTTAGATGAAGAGCA+AGG + chr4.1:75358655-75358674 None:intergenic 40.0%
CCCTGGAAGGAATAACATTT+AGG + chr4.1:75359917-75359936 None:intergenic 40.0%
CCTTGCTCTTCATCTAAGAA+TGG - chr4.1:75358652-75358671 MS.gene87552:intron 40.0%
CTTAGATGAAGAGCAAGGTA+GGG + chr4.1:75358650-75358669 None:intergenic 40.0%
GACTTGGAGGATGAAGAAAA+GGG - chr4.1:75357305-75357324 MS.gene87552:intron 40.0%
GAGGGAAGGATTCAGAAAAT+AGG + chr4.1:75359999-75360018 None:intergenic 40.0%
GATGCAAGAATTTATTGGGC+CGG - chr4.1:75359241-75359260 MS.gene87552:intron 40.0%
GTATTTCTTGTGTCCTGCAT+AGG + chr4.1:75359948-75359967 None:intergenic 40.0%
TACCATGAAAGAAAGATGGC+TGG - chr4.1:75358794-75358813 MS.gene87552:CDS 40.0%
TCAGAATTCATTAGGACAGC+CGG - chr4.1:75360057-75360076 MS.gene87552:CDS 40.0%
TCCCTACTGAAATCCAAACA+AGG + chr4.1:75357386-75357405 None:intergenic 40.0%
TCTTAGATGAAGAGCAAGGT+AGG + chr4.1:75358651-75358670 None:intergenic 40.0%
TGCGAAGAAAGCTTCTCTAA+TGG - chr4.1:75359369-75359388 MS.gene87552:CDS 40.0%
TGCTGTTAAACTCTGCTTCA+GGG + chr4.1:75360021-75360040 None:intergenic 40.0%
! AAAGCTTCTTTTCCACCCAA+TGG + chr4.1:75359274-75359293 None:intergenic 40.0%
! ATGTCATTTGGGGAGATGAA+AGG - chr4.1:75357564-75357583 MS.gene87552:CDS 40.0%
! CCCTTAATCTCAAATGGGTT+TGG - chr4.1:75357041-75357060 MS.gene87552:intron 40.0%
! GATGTTTTCATGCCATCACA+TGG - chr4.1:75359424-75359443 MS.gene87552:CDS 40.0%
! TGACTTGGAGGATGAAGAAA+AGG - chr4.1:75357304-75357323 MS.gene87552:intron 40.0%
! TTTTACGCAGCTTGGATTCA+AGG - chr4.1:75358414-75358433 MS.gene87552:intron 40.0%
!! AGCCTTGTTTGGATTTCAGT+AGG - chr4.1:75357381-75357400 MS.gene87552:intron 40.0%
!! CTATAGGCTTCACTTTTCAG+TGG - chr4.1:75359138-75359157 MS.gene87552:intron 40.0%
!! GCCTTGTTTGGATTTCAGTA+GGG - chr4.1:75357382-75357401 MS.gene87552:intron 40.0%
!! GTTAGGGTATAAGCCTTGTT+TGG - chr4.1:75357370-75357389 MS.gene87552:intron 40.0%
!! TGTTTTGCTTTTACGCAGCT+TGG - chr4.1:75358406-75358425 MS.gene87552:intron 40.0%
!! TTATGGTGGTTGTTTCTGGA+GGG - chr4.1:75357453-75357472 MS.gene87552:CDS 40.0%
!!! AGTTTTGGAAGCAACCAGAT+GGG - chr4.1:75357348-75357367 MS.gene87552:intron 40.0%
AAGCTATCTTGGAGAGCTTG+TGG + chr4.1:75358998-75359017 None:intergenic 45.0%
AAGGAAGATCTTGCATTGCC+TGG - chr4.1:75359331-75359350 MS.gene87552:CDS 45.0%
AGCTATCTTGGAGAGCTTGT+GGG + chr4.1:75358997-75359016 None:intergenic 45.0%
ATAGTCATACCTGAAGCGCA+TGG + chr4.1:75359462-75359481 None:intergenic 45.0%
CAACTTGGAGTGACATGAAG+AGG + chr4.1:75358100-75358119 None:intergenic 45.0%
CCCATATTTCCTCCATGTGA+TGG + chr4.1:75359439-75359458 None:intergenic 45.0%
CCTTCTCTGTTGAACTGCAA+AGG + chr4.1:75358386-75358405 None:intergenic 45.0%
CCTTTGCAGTTCAACAGAGA+AGG - chr4.1:75358383-75358402 MS.gene87552:intron 45.0%
CTCTTCATGTCACTCCAAGT+TGG - chr4.1:75358098-75358117 MS.gene87552:CDS 45.0%
CTTGGATTCAAGGCTATCGA+AGG - chr4.1:75358424-75358443 MS.gene87552:intron 45.0%
GACCAGCCATCTTTCTTTCA+TGG + chr4.1:75358799-75358818 None:intergenic 45.0%
GAGGATGAAGAAAAGGGTGA+GGG - chr4.1:75357311-75357330 MS.gene87552:intron 45.0%
GATCTTCCTTTCAAGAGCTC+CGG + chr4.1:75358764-75358783 None:intergenic 45.0%
GCCATCACATGGAGGAAATA+TGG - chr4.1:75359435-75359454 MS.gene87552:CDS 45.0%
GCGAAACCAGTTCAAGAACT+TGG - chr4.1:75358590-75358609 MS.gene87552:intron 45.0%
GCTATCGAAGGATCTTCCTT+CGG - chr4.1:75358436-75358455 MS.gene87552:CDS 45.0%
GCTGAGAGAATGCAAAGCAA+AGG - chr4.1:75358623-75358642 MS.gene87552:intron 45.0%
GGTGGTGAAGAAGAAGTTAG+AGG + chr4.1:75356883-75356902 None:intergenic 45.0%
GTAACATCCATGGCAGTCAA+GGG + chr4.1:75358827-75358846 None:intergenic 45.0%
GTTAAACTCTGCTTCAGGGA+GGG + chr4.1:75360017-75360036 None:intergenic 45.0%
TCAAACTCAGGACTTAGACC+AGG + chr4.1:75358710-75358729 None:intergenic 45.0%
TCTTCAGAAGCTTCGATGCA+AGG - chr4.1:75358460-75358479 MS.gene87552:CDS 45.0%
TGCAAGAATTTATTGGGCCG+GGG - chr4.1:75359243-75359262 MS.gene87552:intron 45.0%
TGTTAAACTCTGCTTCAGGG+AGG + chr4.1:75360018-75360037 None:intergenic 45.0%
TGTTCTTGCCAATGATGTGC+GGG - chr4.1:75358018-75358037 MS.gene87552:intron 45.0%
TTGCAAGCAAAGGTTCCTAG+TGG - chr4.1:75357278-75357297 MS.gene87552:intron 45.0%
! ACACTTGATGACAAAGCCTG+TGG - chr4.1:75358063-75358082 MS.gene87552:CDS 45.0%
! CTCATCGCACCTATGTTTTC+AGG - chr4.1:75358544-75358563 MS.gene87552:intron 45.0%
! GTTTTCATGCCATCACATGG+AGG - chr4.1:75359427-75359446 MS.gene87552:CDS 45.0%
! TTGATGACAAAGCCTGTGGA+GGG - chr4.1:75358067-75358086 MS.gene87552:CDS 45.0%
!! CTTATGGTGGTTGTTTCTGG+AGG - chr4.1:75357452-75357471 MS.gene87552:CDS 45.0%
!! GCAAATGGTTCTAGTTCTCC+AGG + chr4.1:75359352-75359371 None:intergenic 45.0%
!! TAGGATTCTTGGTGCTGCTT+TGG - chr4.1:75357520-75357539 MS.gene87552:CDS 45.0%
!!! GAGTTTTGGAAGCAACCAGA+TGG - chr4.1:75357347-75357366 MS.gene87552:intron 45.0%
AAATATGGGCCATGCGCTTC+AGG - chr4.1:75359450-75359469 MS.gene87552:CDS 50.0%
ACCTTGCTAGTCCTGAGTTC+CGG + chr4.1:75360079-75360098 None:intergenic 50.0%
AGTCGTTGCACATGCACTCT+GGG + chr4.1:75360129-75360148 None:intergenic 50.0%
CCGTTCGCGCTATCTCAAAA+GGG - chr4.1:75358123-75358142 MS.gene87552:CDS 50.0%
CTTGGAGTGACATGAAGAGG+AGG + chr4.1:75358097-75358116 None:intergenic 50.0%
GAATGAGAGGAGAGAACGAC+CGG - chr4.1:75358742-75358761 MS.gene87552:intron 50.0%
GAGAGCTTGTGGGAATAAGC+TGG + chr4.1:75358987-75359006 None:intergenic 50.0%
GCAAGAATTTATTGGGCCGG+GGG - chr4.1:75359244-75359263 MS.gene87552:intron 50.0%
GGAGGATGAAGAAAAGGGTG+AGG - chr4.1:75357310-75357329 MS.gene87552:intron 50.0%
GGCTTATACCCTAACCCATC+TGG + chr4.1:75357365-75357384 None:intergenic 50.0%
GGTAACATCCATGGCAGTCA+AGG + chr4.1:75358828-75358847 None:intergenic 50.0%
GGTTCCTAGTGGAGTTGACT+TGG - chr4.1:75357289-75357308 MS.gene87552:intron 50.0%
TCCGTTCGCGCTATCTCAAA+AGG - chr4.1:75358122-75358141 MS.gene87552:CDS 50.0%
TCCTCCAAGTCAACTCCACT+AGG + chr4.1:75357296-75357315 None:intergenic 50.0%
TCTTTGTCCCTTGACTGCCA+TGG - chr4.1:75358817-75358836 MS.gene87552:CDS 50.0%
TGACTGCCATGGATGTTACC+AGG - chr4.1:75358828-75358847 MS.gene87552:CDS 50.0%
TGGAAAAGGATGTGTGGGTG+AGG - chr4.1:75358672-75358691 MS.gene87552:intron 50.0%
! AACTCAGGACTAGCAAGGTC+GGG - chr4.1:75360080-75360099 MS.gene87552:CDS 50.0%
! CGAAGCTTCTGAAGATCCGA+AGG + chr4.1:75358455-75358474 None:intergenic 50.0%
! CTTGATGACAAAGCCTGTGG+AGG - chr4.1:75358066-75358085 MS.gene87552:CDS 50.0%
! GAACTCAGGACTAGCAAGGT+CGG - chr4.1:75360079-75360098 MS.gene87552:CDS 50.0%
! TGGAAGCAACCAGATGGGTT+AGG - chr4.1:75357353-75357372 MS.gene87552:intron 50.0%
!! GGAAGCAACCAGATGGGTTA+GGG - chr4.1:75357354-75357373 MS.gene87552:intron 50.0%
!! TCCTAGTGGAGTTGACTTGG+AGG - chr4.1:75357292-75357311 MS.gene87552:intron 50.0%
!!! CCCTTTTGAGATAGCGCGAA+CGG + chr4.1:75358126-75358145 None:intergenic 50.0%
AACTCTGCTTCAGGGAGGGA+AGG + chr4.1:75360013-75360032 None:intergenic 55.0%
AAGGATGTGTGGGTGAGGAC+TGG - chr4.1:75358677-75358696 MS.gene87552:intron 55.0%
ATAGGCTCTGTGACCCTGGA+AGG + chr4.1:75359930-75359949 None:intergenic 55.0%
CATTAGGACAGCCGGAACTC+AGG - chr4.1:75360065-75360084 MS.gene87552:CDS 55.0%
CGAAACCACCCGCACATCAT+TGG + chr4.1:75358029-75358048 None:intergenic 55.0%
CTGGAGGGATGAATCAGCAG+AGG - chr4.1:75357468-75357487 MS.gene87552:CDS 55.0%
GAACGACCGGAGCTCTTGAA+AGG - chr4.1:75358755-75358774 MS.gene87552:intron 55.0%
GAGTCGTTGCACATGCACTC+TGG + chr4.1:75360130-75360149 None:intergenic 55.0%
TAGCGCGAACGGATCCAACT+TGG + chr4.1:75358115-75358134 None:intergenic 55.0%
TCTTGCCAATGATGTGCGGG+TGG - chr4.1:75358021-75358040 MS.gene87552:intron 55.0%
!! GTGAGGACTGGTTGTCTACC+TGG - chr4.1:75358689-75358708 MS.gene87552:intron 55.0%
!! TTGCACATGCACTCTGGGAC+AGG + chr4.1:75360124-75360143 None:intergenic 55.0%
AGAGGAGGACTTCCCTCCAC+AGG + chr4.1:75358082-75358101 None:intergenic 60.0%
CAGGGTCACAGAGCCTATGC+AGG - chr4.1:75359932-75359951 MS.gene87552:CDS 60.0%
CTGCATAGGCTCTGTGACCC+TGG + chr4.1:75359934-75359953 None:intergenic 60.0%
TCCACCCAATGGTTGTCCCC+CGG + chr4.1:75359263-75359282 None:intergenic 60.0%
! CAGGACTAGCAAGGTCGGGA+GGG - chr4.1:75360084-75360103 MS.gene87552:CDS 60.0%
! GCCGGAACTCAGGACTAGCA+AGG - chr4.1:75360075-75360094 MS.gene87552:CDS 60.0%
! TCAGGACTAGCAAGGTCGGG+AGG - chr4.1:75360083-75360102 MS.gene87552:CDS 60.0%
TGGGCCGGGGGACAACCATT+GGG - chr4.1:75359256-75359275 MS.gene87552:intron 65.0%
TTGGGCCGGGGGACAACCAT+TGG - chr4.1:75359255-75359274 MS.gene87552:intron 65.0%
GCCGGGGGACAACCATTGGG+TGG - chr4.1:75359259-75359278 MS.gene87552:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 75356744 75360165 75356744 ID=MS.gene87552
chr4.1 mRNA 75356744 75360165 75356744 ID=MS.gene87552.t1;Parent=MS.gene87552
chr4.1 exon 75359324 75360165 75359324 ID=MS.gene87552.t1.exon1;Parent=MS.gene87552.t1
chr4.1 CDS 75359324 75360165 75359324 ID=cds.MS.gene87552.t1;Parent=MS.gene87552.t1
chr4.1 exon 75358765 75358930 75358765 ID=MS.gene87552.t1.exon2;Parent=MS.gene87552.t1
chr4.1 CDS 75358765 75358930 75358765 ID=cds.MS.gene87552.t1;Parent=MS.gene87552.t1
chr4.1 exon 75358428 75358517 75358428 ID=MS.gene87552.t1.exon3;Parent=MS.gene87552.t1
chr4.1 CDS 75358428 75358517 75358428 ID=cds.MS.gene87552.t1;Parent=MS.gene87552.t1
chr4.1 exon 75358060 75358343 75358060 ID=MS.gene87552.t1.exon4;Parent=MS.gene87552.t1
chr4.1 CDS 75358060 75358343 75358060 ID=cds.MS.gene87552.t1;Parent=MS.gene87552.t1
chr4.1 exon 75357438 75357696 75357438 ID=MS.gene87552.t1.exon5;Parent=MS.gene87552.t1
chr4.1 CDS 75357438 75357696 75357438 ID=cds.MS.gene87552.t1;Parent=MS.gene87552.t1
chr4.1 exon 75356744 75356974 75356744 ID=MS.gene87552.t1.exon6;Parent=MS.gene87552.t1
chr4.1 CDS 75356744 75356974 75356744 ID=cds.MS.gene87552.t1;Parent=MS.gene87552.t1
Gene Sequence

>MS.gene87552

ATGGCTAAGTCAAAGAACAATGCAAAGAAACTCTCATACATCTCAGTTCCTTCACAAATAATCAATTCCATTTCATCTTCTTCTATTCAATCATTACTTGATGATCAAACTCCAAAGAAATCTTCAACAAAAATTGCCTCTAACTTCTTCTTCACCACCTTAACAAAATGGAAAAACCCTTCAAGATTGTTCTTCTTTTTCACACTCTTATTTCTTGGTGTTTTTGCTTTGTTTATGATTGGTTTCAATCTTAAAACACCGTTTTTTAAAACCCCATGTGCTACAAATGTTGAAAAGCCCTTAATCTCAAATGGGTTTGGTTCAAAATCTGAACTTAAGGTTGTTTCAATGAATAAAAATGAGATTTTTAATGGTGATGTTTTGGTAAAATCTGAACTTGGGGTTGTTCAAAACCCTTCAATCTCAAATGGGTTTGATTCAAAATCTGAACTTGGTGCTGTTTCAATGAATAAAGATGAGGTTTTGAATGATGGTAGTTTGTCAAATTCACTTCCTTTGATTGGTAATGTAATGTTGCAAGCAAAGGTTCCTAGTGGAGTTGACTTGGAGGATGAAGAAAAGGGTGAGGGAGTTGAAAAGAGTGAGTTTTGGAAGCAACCAGATGGGTTAGGGTATAAGCCTTGTTTGGATTTCAGTAGGGATTATAGAAGAGAAAGTGATGGGATTGTGAAGAATAGAAAAAGGTATCTTATGGTGGTTGTTTCTGGAGGGATGAATCAGCAGAGGAATCAAATTGTTGATGCTGTTGTTATTGCTAGGATTCTTGGTGCTGCTTTGGTTGTTCCTATTTTGCAAGTTAATGTCATTTGGGGAGATGAAAGGTAAAGTTTACTATTCTTCTTGTTCATGGTTTGTGATATTGTAAGTGATGATTTTAATCAATGAAGATTTATGTAAGGTTTAGTTATTACAATTTTGGACTTAATCTAAGCCTAGGCATAGATTGATCTTGTTTCAATATTGTTTGAAAGACCAAATTTGGATATATATTTAGCTGTTACAAATTTGGACTGAATCTTGAGAAAGAACAACTTTGGAGCTTAAGCTTTAAGCATAGTTCTATTCTAGCTATAGTTTCAATCTTTTTGTTCTTGATACTGGTTATGTTTCGTTGTTAATGTTGATTAATTTCTTTCCTTATTTGTGAATGCTAGAAGAGAGTTTTCTGTAATTTATTATATTTTTTTCTCATTGGTTTTGGTTTTGTTTTGCAGTGAATTTGCTGATATATTTGATTTTGAGCACTTTAAGAATGTTCTTGCCAATGATGTGCGGGTGGTTTCGTCATTGCCATCAACACACTTGATGACAAAGCCTGTGGAGGGAAGTCCTCCTCTTCATGTCACTCCAAGTTGGATCCGTTCGCGCTATCTCAAAAGGGTAAGATTTTTTTGTTAATTACTCATGTTTTTGCATCTTTAGGTTTCAGTCCTAAGTTGTTGCTAAGTTTTTGTCTACTTTTGCGTTGAAAAATTTCAGACAATGATTGTCTCTTTGATGAGAAAGTGTGTTAACTTGTATATAATCATGTTAACTAATGAAAGCTTTGACTTCTGTGGAAATAAAAATTTGGGCAGTGAGTGATTGTTGAGCTTGAGTTTAACATGATCTTTGTTGTCCTTTGCAGTTCAACAGAGAAGGTGTTTTGCTTTTACGCAGCTTGGATTCAAGGCTATCGAAGGATCTTCCTTCGGATCTTCAGAAGCTTCGATGCAAGGTACTGTGAAGTCTCTATAGTATCTTCATTTGTGTAACAATCGGTAGCATATTCTAAATGTTCTCATCGCACCTATGTTTTCAGGTTGCTTTTAGTGCATTGAGATTTGCGAAACCAGTTCAAGAACTTGGAAACAAGATTGCTGAGAGAATGCAAAGCAAAGGACCCTACCTTGCTCTTCATCTAAGAATGGAAAAGGATGTGTGGGTGAGGACTGGTTGTCTACCTGGTCTAAGTCCTGAGTTTGACGAGATTGTAAAGAATGAGAGGAGAGAACGACCGGAGCTCTTGAAAGGAAGATCAAACATGACTTACCATGAAAGAAAGATGGCTGGTCTTTGTCCCTTGACTGCCATGGATGTTACCAGGTAAATATTAAATTCTACTTTTGTATAGGAGTCTGTTTGGATTCTCTTATTCGAGTTTATTACTGGCATTTACACTTATGAAACTATTTGGGAGAGCTTATGCAAACAACTTATGACATGTCCATAAACTGTTTCCAGCTTATTCCCACAAGCTCTCCAAGATAGCTTATGAAATCAGTTTAGTTTATATAAAAATAGTTCGATTTTATTTTATCTTTTATTATAGAAATAGCGTATACATAAACACTTATGCTACAAGCGCTTAATTAAGTTGTTTATCCAAAGATCCTATAGGCTTCACTTTTCAGTGGTTTTCTGAATATTAGTGTGACAGAGTTTTGTTTATTTGTTTTGGATTTATAGGTTGCTTAAAGCTTTAGGAGCTCAAAAAGATGCAAGAATTTATTGGGCCGGGGGACAACCATTGGGTGGAAAAGAAGCTTTGCATCCATTAATCCATGAGTTTCCACATCTTTATAATAAGGAAGATCTTGCATTGCCTGGAGAACTAGAACCATTTGCGAAGAAAGCTTCTCTAATGGCTGCCATAGATTACATAGTTTCTGAGAAGAGTGATGTTTTCATGCCATCACATGGAGGAAATATGGGCCATGCGCTTCAGGTATGACTATATAATGACAGCAAGAGTGCTTTGCAATTAAGATACTCACATAAGCATTGATGATTGTTGCATAACTTTATTGTAATCATGCTTACATATATTGGCCATGCATTCATATATACTGTATTCACACATATTTTATAAGAAAAGATTGCGACCTTTCAACTTTAGTCCCTATAAAATTTGTACTCACCTTTAGTCTTCATATTTAATAAAAATAGTGTGCTTTTAGTAATTAAAAAGGACTGAGGATACAATCAATTATTTTAGGGATGTTGATGAGTCGTGATTTTATAGGACCAAAAACATATTTTCTCCATAAATTGTAATCATGCAATTAAGATAGTCACATTGATGGATGTTGCATAAATATATTGTAAATGTAATAATGCATACGCATATTTAATGTAGATTCTTCATTTGACAATTTCTAATATCTGAACCTAAATGTTATTCCTTCCAGGGTCACAGAGCCTATGCAGGACACAAGAAATACATAACCCCAAACAAAAGACAAATGCATCCCTATTTTCTGAATCCTTCCCTCCCTGAAGCAGAGTTTAACAGCATTGTCAAAGAATTGCATCAGAATTCATTAGGACAGCCGGAACTCAGGACTAGCAAGGTCGGGAGGGATGTCACGAAGTATCCTGTCCCAGAGTGCATGTGCAACGACTCTCATAATGATTCGTAG
Protein sequence

>MS.gene87552.t1

MAKSKNNAKKLSYISVPSQIINSISSSSIQSLLDDQTPKKSSTKIASNFFFTTLTKWKNPSRLFFFFTLLFLGVFALFMIGFNLKTPFFKTPCATNVEKPLISNGFGSKSELKVVSMNKNEIFNGDVLVKSELGVVQNPSISNGFDSKSELGAVSMNKDEVLNDGSLSNSLPLIGNVMLQAKVPSGVDLEDEEKGEGVEKSEFWKQPDGLGYKPCLDFSRDYRRESDGIVKNRKRYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFADIFDFEHFKNVLANDVRVVSSLPSTHLMTKPVEGSPPLHVTPSWIRSRYLKRFNREGVLLLRSLDSRLSKDLPSDLQKLRCKVAFSALRFAKPVQELGNKIAERMQSKGPYLALHLRMEKDVWVRTGCLPGLSPEFDEIVKNERRERPELLKGRSNMTYHERKMAGLCPLTAMDVTRLLKALGAQKDARIYWAGGQPLGGKEALHPLIHEFPHLYNKEDLALPGELEPFAKKASLMAAIDYIVSEKSDVFMPSHGGNMGHALQGHRAYAGHKKYITPNKRQMHPYFLNPSLPEAEFNSIVKELHQNSLGQPELRTSKVGRDVTKYPVPECMCNDSHNDS