AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88661


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88661.t1 MTR_4g128570 96.667 180 6 0 3 182 96 275 2.33e-133 374
MS.gene88661.t1 MTR_7g093530 79.581 191 25 1 3 179 89 279 2.40e-112 321
MS.gene88661.t1 MTR_4g128580 78.788 198 24 1 3 182 91 288 5.26e-112 320
MS.gene88661.t1 MTR_4g128410 78.534 191 27 1 3 179 90 280 1.83e-109 314
MS.gene88661.t1 MTR_4g128590 74.372 199 32 1 3 182 80 278 6.59e-108 310
MS.gene88661.t1 MTR_4g128400 69.948 193 44 1 3 181 93 285 1.15e-100 292
MS.gene88661.t1 MTR_2g038860 67.839 199 45 1 3 182 91 289 1.47e-97 284
MS.gene88661.t1 MTR_2g038705 67.839 199 45 1 3 182 91 289 1.47e-97 284
MS.gene88661.t1 MTR_4g126920 66.667 195 50 1 3 182 90 284 8.49e-96 279
MS.gene88661.t1 MTR_3g084620 71.351 185 45 1 3 179 93 277 1.72e-94 276
MS.gene88661.t1 MTR_3g084630 71.351 185 45 1 3 179 93 277 2.30e-94 276
MS.gene88661.t1 MTR_8g081040 68.984 187 49 1 3 180 96 282 1.08e-93 274
MS.gene88661.t1 MTR_8g102220 65.027 183 54 2 3 182 94 269 2.48e-82 245
MS.gene88661.t1 MTR_8g102910 54.359 195 71 2 3 179 85 279 3.25e-73 222
MS.gene88661.t1 MTR_6g088320 52.821 195 74 2 3 179 89 283 5.59e-72 219
MS.gene88661.t1 MTR_6g033055 55.440 193 70 3 3 180 97 288 5.00e-70 214
MS.gene88661.t1 MTR_6g033085 55.440 193 70 3 3 180 97 288 5.76e-70 214
MS.gene88661.t1 MTR_7g084770 55.208 192 70 3 3 179 97 287 1.12e-69 213
MS.gene88661.t1 MTR_7g084760 55.208 192 70 3 3 179 97 287 1.67e-69 213
MS.gene88661.t1 MTR_7g084750 54.167 192 72 3 3 179 97 287 7.35e-69 211
MS.gene88661.t1 MTR_2g089140 50.249 201 76 4 3 180 25 224 9.19e-69 209
MS.gene88661.t1 MTR_2g089140 50.249 201 76 4 3 180 88 287 5.01e-68 209
MS.gene88661.t1 MTR_3g089540 50.532 188 82 1 3 179 95 282 5.24e-68 209
MS.gene88661.t1 MTR_5g091730 49.462 186 85 1 3 179 93 278 6.84e-67 206
MS.gene88661.t1 MTR_4g045853 49.751 201 77 4 3 180 90 289 7.30e-66 204
MS.gene88661.t1 MTR_1g105900 50.000 194 81 2 3 180 99 292 1.41e-62 195
MS.gene88661.t1 MTR_4g097640 50.538 186 77 4 11 182 106 290 2.39e-62 194
MS.gene88661.t1 MTR_4g097670 50.538 186 77 4 11 182 106 290 2.39e-62 194
MS.gene88661.t1 MTR_4g097700 50.811 185 76 4 11 181 143 326 7.33e-62 194
MS.gene88661.t1 MTR_8g013055 46.875 192 85 4 3 179 96 285 1.33e-59 187
MS.gene88661.t1 MTR_1g105495 49.468 188 78 3 11 182 111 297 1.82e-59 187
MS.gene88661.t1 MTR_7g056090 48.837 172 69 5 11 167 106 273 3.23e-48 158
MS.gene88661.t1 MTR_2g049030 44.324 185 81 6 11 177 101 281 6.00e-46 152
MS.gene88661.t1 MTR_7g102260 42.781 187 87 5 11 181 111 293 2.44e-44 149
MS.gene88661.t1 MTR_4g057450 43.316 187 86 5 9 179 122 304 1.44e-43 147
MS.gene88661.t1 MTR_1g063020 43.784 185 84 7 11 179 114 294 5.62e-43 145
MS.gene88661.t1 MTR_2g095800 42.857 182 83 6 6 170 124 301 6.34e-43 145
MS.gene88661.t1 MTR_2g049130 42.308 182 80 5 3 166 95 269 5.16e-42 142
MS.gene88661.t1 MTR_1g033420 37.234 188 100 4 9 180 107 292 6.86e-39 135
MS.gene88661.t1 MTR_5g029100 35.263 190 100 6 11 179 87 274 1.35e-37 132
MS.gene88661.t1 MTR_5g029100 35.263 190 100 6 11 179 118 305 5.13e-37 131
MS.gene88661.t1 MTR_8g090100 35.135 185 103 4 11 180 100 282 2.08e-35 126
MS.gene88661.t1 MTR_8g064180 33.161 193 105 5 9 179 120 310 5.48e-31 114
MS.gene88661.t1 MTR_8g479340 40.404 99 42 3 100 182 41 138 1.60e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88661.t1 AT4G25810 72.449 196 38 1 3 182 91 286 2.43e-106 306
MS.gene88661.t1 AT5G57560 70.352 199 42 1 1 182 86 284 1.16e-105 304
MS.gene88661.t1 AT4G30270 75.000 180 45 0 3 182 89 268 1.94e-105 303
MS.gene88661.t1 AT5G57550 67.021 188 51 1 3 179 95 282 8.35e-98 285
MS.gene88661.t1 AT4G30290 64.362 188 57 1 3 180 90 277 3.11e-93 273
MS.gene88661.t1 AT4G30280 63.298 188 59 1 3 180 95 282 1.17e-92 271
MS.gene88661.t1 AT5G57530 65.969 191 51 1 3 179 92 282 3.64e-92 270
MS.gene88661.t1 AT1G65310 63.298 188 59 1 3 180 95 282 5.82e-92 270
MS.gene88661.t1 AT1G65310 63.298 188 59 1 3 180 117 304 6.23e-92 270
MS.gene88661.t1 AT5G48070 61.702 188 62 1 3 180 95 282 3.53e-91 268
MS.gene88661.t1 AT5G57540 64.921 191 53 1 3 179 91 281 5.07e-91 267
MS.gene88661.t1 AT2G18800 61.165 206 53 3 3 181 93 298 1.20e-89 265
MS.gene88661.t1 AT3G23730 64.141 198 53 2 3 182 92 289 9.04e-89 262
MS.gene88661.t1 AT4G14130 63.077 195 57 1 3 182 93 287 2.17e-88 261
MS.gene88661.t1 AT4G25820 63.351 191 56 1 3 179 95 285 1.27e-87 259
MS.gene88661.t1 AT5G13870 57.812 192 65 3 3 179 97 287 3.72e-71 217
MS.gene88661.t1 AT5G13870 57.812 192 65 3 3 179 97 287 3.72e-71 217
MS.gene88661.t1 AT5G13870 57.812 192 65 3 3 179 97 287 3.72e-71 217
MS.gene88661.t1 AT2G06850 55.208 192 70 3 3 179 40 230 1.57e-69 211
MS.gene88661.t1 AT4G28850 51.269 197 77 2 3 180 92 288 7.72e-69 211
MS.gene88661.t1 AT2G06850 55.208 192 70 3 3 179 100 290 9.18e-69 211
MS.gene88661.t1 AT4G03210 51.031 194 77 3 3 179 64 256 2.75e-66 204
MS.gene88661.t1 AT4G03210 51.031 194 77 3 3 179 92 284 5.24e-66 204
MS.gene88661.t1 AT5G65730 50.811 185 76 3 11 181 109 292 5.57e-65 201
MS.gene88661.t1 AT2G14620 49.744 195 80 4 3 180 41 234 3.20e-61 190
MS.gene88661.t1 AT2G14620 49.744 195 80 4 3 180 52 245 3.75e-61 190
MS.gene88661.t1 AT4G37800 50.270 185 77 3 11 181 108 291 5.91e-61 191
MS.gene88661.t1 AT2G14620 49.744 195 80 4 3 180 102 295 1.57e-60 190
MS.gene88661.t1 AT1G11545 48.969 194 80 4 7 182 110 302 1.25e-59 188
MS.gene88661.t1 AT4G13090 46.907 194 84 4 3 179 97 288 2.01e-56 179
MS.gene88661.t1 AT3G25050 46.392 194 83 5 3 179 100 289 4.24e-54 174
MS.gene88661.t1 AT4G13080 46.597 191 85 5 3 179 104 291 1.40e-53 172
MS.gene88661.t1 AT3G44990 44.318 176 79 5 11 170 113 285 2.32e-44 149
MS.gene88661.t1 AT2G36870 43.243 185 85 5 11 179 117 297 4.35e-43 145
MS.gene88661.t1 AT1G32170 37.566 189 97 5 11 180 108 294 1.18e-42 145
MS.gene88661.t1 AT1G14720 35.263 190 104 4 9 181 105 292 5.54e-38 133
MS.gene88661.t1 AT2G01850 34.737 190 105 4 9 181 105 292 5.26e-37 130
MS.gene88661.t1 AT4G18990 33.000 200 102 5 11 180 42 239 2.45e-35 125
MS.gene88661.t1 AT4G18990 34.500 200 99 5 11 180 116 313 1.50e-34 125
MS.gene88661.t1 AT1G10550 33.846 195 101 5 11 179 117 309 7.38e-33 119
MS.gene88661.t1 AT3G48580 34.921 189 98 6 3 179 39 214 1.40e-31 114
MS.gene88661.t1 AT3G48580 34.921 189 98 6 3 179 98 273 5.62e-31 114
MS.gene88661.t1 AT2G36870 56.452 62 22 1 11 67 117 178 1.31e-18 79.3
MS.gene88661.t1 AT3G48580 28.042 189 91 7 3 179 98 253 2.14e-14 69.3

Find 41 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCTCATTGGTTGATTCTT+TGG 0.185511 8.1:+4403413 None:intergenic
ATGTTCTTGAATTCTCTTAT+TGG 0.197118 8.1:+4403458 None:intergenic
TTCACACCTACTCCATTCTT+TGG 0.237903 8.1:-4403657 MS.gene88661:CDS
TGAAATTGACTTTGAATTCT+TGG 0.248522 8.1:-4403785 MS.gene88661:CDS
TGTCATCAAAAGGATCAAAT+TGG 0.258251 8.1:-4403810 MS.gene88661:CDS
CTCCATTCACTGCATCTTAT+AGG 0.269112 8.1:-4403319 MS.gene88661:CDS
GAAATTGACTTTGAATTCTT+GGG 0.327454 8.1:-4403784 MS.gene88661:CDS
GAGAGCAACAATTCCATCTA+TGG 0.342180 8.1:-4403699 MS.gene88661:CDS
TGAGGATATACTCAAGTCTA+TGG 0.355078 8.1:-4403397 MS.gene88661:CDS
TAGGATGCAGTGCACTCTAT+TGG 0.390519 8.1:+4403152 None:intergenic
CAGTTGGATCAAACCATAGA+TGG 0.393171 8.1:+4403686 None:intergenic
TGCACTCTATTGGAAGGCCT+TGG 0.405324 8.1:+4403162 None:intergenic
GCACTCTATTGGAAGGCCTT+GGG 0.408314 8.1:+4403163 None:intergenic
ACTTGTTGAATCCAACTCTT+GGG 0.425232 8.1:+4403256 None:intergenic
AGACTTGAGTATATCCTCAT+TGG 0.432038 8.1:+4403401 None:intergenic
GTCTATGGAATGCTGATGAT+TGG 0.434923 8.1:-4403382 MS.gene88661:CDS
GACTTGTTGAATCCAACTCT+TGG 0.435704 8.1:+4403255 None:intergenic
GTCATCAAAAGGATCAAATT+GGG 0.451206 8.1:-4403809 MS.gene88661:CDS
GGCATGCAGTTGTCATCAAA+AGG 0.488525 8.1:-4403820 MS.gene88661:CDS
GAATTCTTGGGGAATGTAAG+TGG 0.491509 8.1:-4403772 MS.gene88661:CDS
TTCAAGAACATGGAATCAAA+AGG 0.493604 8.1:-4403446 MS.gene88661:CDS
TTCCTATAAGATGCAGTGAA+TGG 0.501342 8.1:+4403317 None:intergenic
GCGGTCTTGTCAAGACAGAT+TGG 0.512024 8.1:-4403349 MS.gene88661:CDS
AATGTGTTTAGTCAAGGAAA+AGG 0.525149 8.1:-4403727 MS.gene88661:CDS
TCTATGGAATGCTGATGATT+GGG 0.538637 8.1:-4403381 MS.gene88661:CDS
CACTCTATTGGAAGGCCTTG+GGG 0.539602 8.1:+4403164 None:intergenic
GCGTTGAGGATTCCAAAGAA+TGG 0.542490 8.1:+4403645 None:intergenic
CACACCAATGTGTTTAGTCA+AGG 0.543031 8.1:-4403733 MS.gene88661:CDS
AAATTGACTTTGAATTCTTG+GGG 0.549232 8.1:-4403783 MS.gene88661:CDS
ACATTGGTGTGAAGAGTGTA+TGG 0.556406 8.1:+4403745 None:intergenic
AGGATTCCAAAGAATGGAGT+AGG 0.588075 8.1:+4403651 None:intergenic
AGACTACTTCATCTAATGCA+TGG 0.591786 8.1:-4403283 MS.gene88661:CDS
TAGGTGTGAAAATCAGCAGT+TGG 0.604383 8.1:+4403670 None:intergenic
ATGCAGTGCACTCTATTGGA+AGG 0.605313 8.1:+4403156 None:intergenic
AATAAGAGAATTCAAGAACA+TGG 0.612760 8.1:-4403456 MS.gene88661:CDS
GATGATTGGGCAACAAGAGG+CGG 0.625561 8.1:-4403368 MS.gene88661:CDS
GCTGATGATTGGGCAACAAG+AGG 0.626727 8.1:-4403371 MS.gene88661:CDS
GGTGCTTACACAATGCGTTG+AGG 0.644219 8.1:+4403631 None:intergenic
TTCCAAAGAATCAACCAATG+AGG 0.645428 8.1:-4403415 MS.gene88661:CDS
AAGTCAACAAAGATTGAGTG+AGG 0.654429 8.1:-4403237 MS.gene88661:CDS
GACCTCAAAAGATTTCCCCA+AGG 0.701224 8.1:-4403179 MS.gene88661:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTAAAAAAAAAATCAAT+TGG - chr8.1:4403422-4403441 MS.gene88661:CDS 10.0%
!! ACTAATATATAAACATGTGA+CGG - chr8.1:4403377-4403396 MS.gene88661:CDS 20.0%
!! TCATCAATAATCAATTCATT+GGG + chr8.1:4403449-4403468 None:intergenic 20.0%
!! TTCATCAATAATCAATTCAT+TGG + chr8.1:4403450-4403469 None:intergenic 20.0%
! AATAAGAGAATTCAAGAACA+TGG - chr8.1:4403512-4403531 MS.gene88661:intron 25.0%
! ATGTTCTTGAATTCTCTTAT+TGG + chr8.1:4403513-4403532 None:intergenic 25.0%
!! AAATTGACTTTGAATTCTTG+GGG - chr8.1:4403185-4403204 MS.gene88661:CDS 25.0%
!! GAAATTGACTTTGAATTCTT+GGG - chr8.1:4403184-4403203 MS.gene88661:CDS 25.0%
!! TGAAATTGACTTTGAATTCT+TGG - chr8.1:4403183-4403202 MS.gene88661:CDS 25.0%
AATGTGTTTAGTCAAGGAAA+AGG - chr8.1:4403241-4403260 MS.gene88661:CDS 30.0%
GTCATCAAAAGGATCAAATT+GGG - chr8.1:4403159-4403178 MS.gene88661:CDS 30.0%
TGTCATCAAAAGGATCAAAT+TGG - chr8.1:4403158-4403177 MS.gene88661:CDS 30.0%
TTCAAGAACATGGAATCAAA+AGG - chr8.1:4403522-4403541 MS.gene88661:intron 30.0%
! AATTGAATGCAGTTTTTCTG+TGG - chr8.1:4403479-4403498 MS.gene88661:CDS 30.0%
! TTTTCCTTGACTAAACACAT+TGG + chr8.1:4403242-4403261 None:intergenic 30.0%
AAGTCAACAAAGATTGAGTG+AGG - chr8.1:4403731-4403750 MS.gene88661:CDS 35.0%
AGACTACTTCATCTAATGCA+TGG - chr8.1:4403685-4403704 MS.gene88661:CDS 35.0%
AGACTTGAGTATATCCTCAT+TGG + chr8.1:4403570-4403589 None:intergenic 35.0%
TCTATGGAATGCTGATGATT+GGG - chr8.1:4403587-4403606 MS.gene88661:intron 35.0%
TGAGGATATACTCAAGTCTA+TGG - chr8.1:4403571-4403590 MS.gene88661:intron 35.0%
TTCCAAAGAATCAACCAATG+AGG - chr8.1:4403553-4403572 MS.gene88661:intron 35.0%
TTCCTATAAGATGCAGTGAA+TGG + chr8.1:4403654-4403673 None:intergenic 35.0%
! ACTTGTTGAATCCAACTCTT+GGG + chr8.1:4403715-4403734 None:intergenic 35.0%
! ATCCTCATTGGTTGATTCTT+TGG + chr8.1:4403558-4403577 None:intergenic 35.0%
AGGATTCCAAAGAATGGAGT+AGG + chr8.1:4403320-4403339 None:intergenic 40.0%
CACACCAATGTGTTTAGTCA+AGG - chr8.1:4403235-4403254 MS.gene88661:CDS 40.0%
CAGTTGGATCAAACCATAGA+TGG + chr8.1:4403285-4403304 None:intergenic 40.0%
CTCCATTCACTGCATCTTAT+AGG - chr8.1:4403649-4403668 MS.gene88661:CDS 40.0%
GAATTCTTGGGGAATGTAAG+TGG - chr8.1:4403196-4403215 MS.gene88661:CDS 40.0%
GAGAGCAACAATTCCATCTA+TGG - chr8.1:4403269-4403288 MS.gene88661:CDS 40.0%
GTCTATGGAATGCTGATGAT+TGG - chr8.1:4403586-4403605 MS.gene88661:intron 40.0%
TAGGTGTGAAAATCAGCAGT+TGG + chr8.1:4403301-4403320 None:intergenic 40.0%
TTCACACCTACTCCATTCTT+TGG - chr8.1:4403311-4403330 MS.gene88661:CDS 40.0%
! ACATTGGTGTGAAGAGTGTA+TGG + chr8.1:4403226-4403245 None:intergenic 40.0%
! GAATGCAGTTTTTCTGTGGA+TGG - chr8.1:4403483-4403502 MS.gene88661:intron 40.0%
!! GACTTGTTGAATCCAACTCT+TGG + chr8.1:4403716-4403735 None:intergenic 40.0%
AACGCATTGTGTAAGCACCT+CGG - chr8.1:4403341-4403360 MS.gene88661:CDS 45.0%
ATGCAGTGCACTCTATTGGA+AGG + chr8.1:4403815-4403834 None:intergenic 45.0%
GACCTCAAAAGATTTCCCCA+AGG - chr8.1:4403789-4403808 MS.gene88661:CDS 45.0%
GCGTTGAGGATTCCAAAGAA+TGG + chr8.1:4403326-4403345 None:intergenic 45.0%
TAGGATGCAGTGCACTCTAT+TGG + chr8.1:4403819-4403838 None:intergenic 45.0%
TAGTGTCAATATCGTGTCCG+AGG + chr8.1:4403361-4403380 None:intergenic 45.0%
! TGCATGGTTTTCCCAAGAGT+TGG - chr8.1:4403701-4403720 MS.gene88661:CDS 45.0%
CACTCTATTGGAAGGCCTTG+GGG + chr8.1:4403807-4403826 None:intergenic 50.0%
GATGATTGGGCAACAAGAGG+CGG - chr8.1:4403600-4403619 MS.gene88661:intron 50.0%
GCACTCTATTGGAAGGCCTT+GGG + chr8.1:4403808-4403827 None:intergenic 50.0%
GCTGATGATTGGGCAACAAG+AGG - chr8.1:4403597-4403616 MS.gene88661:intron 50.0%
GGTGCTTACACAATGCGTTG+AGG + chr8.1:4403340-4403359 None:intergenic 50.0%
TGCACTCTATTGGAAGGCCT+TGG + chr8.1:4403809-4403828 None:intergenic 50.0%
! GCGGTCTTGTCAAGACAGAT+TGG - chr8.1:4403619-4403638 MS.gene88661:intron 50.0%
! GGCCTTGGGGAAATCTTTTG+AGG + chr8.1:4403794-4403813 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 4403151 4403839 4403151 ID=MS.gene88661
chr8.1 mRNA 4403151 4403839 4403151 ID=MS.gene88661.t1;Parent=MS.gene88661
chr8.1 exon 4403640 4403839 4403640 ID=MS.gene88661.t1.exon1;Parent=MS.gene88661.t1
chr8.1 CDS 4403640 4403839 4403640 ID=cds.MS.gene88661.t1;Parent=MS.gene88661.t1
chr8.1 exon 4403151 4403499 4403151 ID=MS.gene88661.t1.exon2;Parent=MS.gene88661.t1
chr8.1 CDS 4403151 4403499 4403151 ID=cds.MS.gene88661.t1;Parent=MS.gene88661.t1
Gene Sequence

>MS.gene88661

ATGCAGTTGTCATCAAAAGGATCAAATTGGGATGAAATTGACTTTGAATTCTTGGGGAATGTAAGTGGAGAACCATACACTCTTCACACCAATGTGTTTAGTCAAGGAAAAGGCAACAGAGAGCAACAATTCCATCTATGGTTTGATCCAACTGCTGATTTTCACACCTACTCCATTCTTTGGAATCCTCAACGCATTGTGTAAGCACCTCGGACACGATATTGACACTAATATATAAACATGTGACGGTGTTTGATAGATACTAGAATTTCTTTTAAAAAAAAAATCAATTGGACCCAATGAATTGATTATTGATGAACATGTGTTTAATTGAATGCAGTTTTTCTGTGGATGGAACACCAATAAGAGAATTCAAGAACATGGAATCAAAAGGAGTTGCATTTCCAAAGAATCAACCAATGAGGATATACTCAAGTCTATGGAATGCTGATGATTGGGCAACAAGAGGCGGTCTTGTCAAGACAGATTGGACAAATGCTCCATTCACTGCATCTTATAGGAATTTCAATGCAGAGACTACTTCATCTAATGCATGGTTTTCCCAAGAGTTGGATTCAACAAGTCAACAAAGATTGAGTGAGGTTCAGAAGAATTACATGATTTACAATTATTGCAGTGACCTCAAAAGATTTCCCCAAGGCCTTCCAATAGAGTGCACTGCATCCTAA

Protein sequence

>MS.gene88661.t1

MQLSSKGSNWDEIDFEFLGNVSGEPYTLHTNVFSQGKGNREQQFHLWFDPTADFHTYSILWNPQRIVFSVDGTPIREFKNMESKGVAFPKNQPMRIYSSLWNADDWATRGGLVKTDWTNAPFTASYRNFNAETTSSNAWFSQELDSTSQQRLSEVQKNYMIYNYCSDLKRFPQGLPIECTAS