AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88984

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88984.t1 MTR_1g026410 98.527 543 7 1 1 542 1 543 0.0 1088
MS.gene88984.t1 MTR_3g115050 84.095 547 79 2 1 542 1 544 0.0 962
MS.gene88984.t1 MTR_4g075690 76.296 540 121 2 3 542 11 543 0.0 857
MS.gene88984.t1 MTR_4g049390 74.031 516 134 0 23 538 22 537 0.0 810
MS.gene88984.t1 MTR_4g048350 72.481 516 141 1 23 538 22 536 0.0 789
MS.gene88984.t1 MTR_2g088080 71.082 536 155 0 2 537 4 539 0.0 784
MS.gene88984.t1 MTR_4g132320 69.963 536 160 1 7 542 11 545 0.0 757
MS.gene88984.t1 MTR_4g075690 74.324 444 107 2 3 446 11 447 0.0 679
MS.gene88984.t1 MTR_2g088080 70.961 458 133 0 80 537 1 458 0.0 669
MS.gene88984.t1 MTR_8g479390 60.155 517 203 2 23 538 26 540 0.0 647
MS.gene88984.t1 MTR_5g018270 58.880 518 209 2 21 538 24 537 0.0 620
MS.gene88984.t1 MTR_8g479380 56.226 530 223 5 6 534 13 534 0.0 615
MS.gene88984.t1 MTR_4g083190 55.970 536 224 5 5 532 7 538 0.0 591
MS.gene88984.t1 MTR_3g083440 54.074 540 230 5 1 532 7 536 0.0 572
MS.gene88984.t1 MTR_3g083390 54.074 540 230 5 1 532 7 536 0.0 571
MS.gene88984.t1 MTR_3g083430 53.889 540 231 5 1 532 7 536 0.0 569
MS.gene88984.t1 MTR_3g083400 53.889 540 231 5 1 532 7 536 0.0 568
MS.gene88984.t1 MTR_3g083450 55.086 521 225 4 20 532 18 537 0.0 566
MS.gene88984.t1 MTR_4g083210 53.131 527 242 4 10 532 7 532 0.0 565
MS.gene88984.t1 MTR_3g083410 54.472 492 215 4 49 532 10 500 0.0 526
MS.gene88984.t1 MTR_4g101650 51.371 547 245 6 7 533 6 551 0.0 526
MS.gene88984.t1 MTR_3g008580 48.644 553 262 7 1 532 1 552 6.49e-175 506
MS.gene88984.t1 MTR_7g051440 51.985 529 228 10 25 532 26 549 1.60e-173 503
MS.gene88984.t1 MTR_1g090707 45.221 544 279 8 3 533 1 538 6.27e-156 458
MS.gene88984.t1 MTR_4g133150 57.212 208 70 2 132 337 21 211 6.19e-70 223
MS.gene88984.t1 MTR_5g020620 29.630 567 345 18 3 531 11 561 6.51e-58 202
MS.gene88984.t1 MTR_5g020600 28.169 568 353 16 6 531 12 566 6.60e-56 197
MS.gene88984.t1 MTR_3g462760 30.040 506 309 15 28 497 26 522 5.27e-53 189
MS.gene88984.t1 MTR_5g081810 27.374 537 341 12 33 531 4 529 1.88e-52 187
MS.gene88984.t1 MTR_5g069680 26.930 531 344 14 1 497 4 524 7.47e-50 180
MS.gene88984.t1 MTR_3g078740 26.065 587 334 20 28 537 26 589 9.05e-48 175
MS.gene88984.t1 MTR_8g091390 26.910 576 327 18 23 536 29 572 1.33e-47 174
MS.gene88984.t1 MTR_4g015120 27.447 521 346 12 22 511 18 537 8.61e-47 172
MS.gene88984.t1 MTR_3g078700 26.850 581 321 23 5 532 7 536 1.39e-45 168
MS.gene88984.t1 MTR_7g458880 25.945 582 365 17 1 531 14 580 1.92e-43 163
MS.gene88984.t1 MTR_3g101640 27.675 542 327 17 6 497 11 537 9.15e-40 152
MS.gene88984.t1 MTR_5g073210 25.958 574 359 16 7 531 4 560 1.36e-39 152
MS.gene88984.t1 MTR_8g027375 25.230 543 362 14 3 504 5 544 2.20e-39 151
MS.gene88984.t1 MTR_7g058690 26.679 521 324 18 28 497 33 546 2.55e-39 151
MS.gene88984.t1 MTR_2g008330 26.376 527 338 14 49 531 59 579 1.61e-38 149
MS.gene88984.t1 MTR_7g065980 28.486 502 308 18 42 496 39 536 1.90e-38 149
MS.gene88984.t1 MTR_3g071890 26.547 501 320 14 49 508 54 547 7.23e-38 147
MS.gene88984.t1 MTR_7g062310 25.528 521 329 15 28 497 47 559 1.95e-37 146
MS.gene88984.t1 MTR_7g060460 25.275 546 345 18 8 497 9 547 3.53e-37 145
MS.gene88984.t1 MTR_7g062250 25.263 570 373 17 11 531 13 578 4.00e-37 145
MS.gene88984.t1 MTR_5g083360 25.651 499 317 14 46 497 42 533 4.42e-37 145
MS.gene88984.t1 MTR_3g101635 28.189 486 311 14 49 497 48 532 5.46e-37 144
MS.gene88984.t1 MTR_3g078730 25.690 580 329 21 9 537 11 539 6.94e-37 144
MS.gene88984.t1 MTR_7g065970 26.325 547 330 18 1 491 1 530 1.93e-35 140
MS.gene88984.t1 MTR_4g064530 24.630 540 361 17 2 497 14 551 1.09e-34 138
MS.gene88984.t1 MTR_4g064530 24.627 536 356 18 2 491 14 547 2.78e-32 130
MS.gene88984.t1 MTR_4g019225 24.864 551 355 17 32 531 30 572 9.50e-28 117
MS.gene88984.t1 MTR_4g133160 52.294 109 38 2 413 521 1 95 5.82e-27 105
MS.gene88984.t1 MTR_5g018290 63.636 66 24 0 473 538 5 70 2.41e-24 96.7
MS.gene88984.t1 MTR_5g081790 26.538 260 158 7 256 489 168 420 4.87e-14 74.7
MS.gene88984.t1 MTR_5g081790 24.242 165 116 3 10 166 6 169 1.08e-11 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88984.t1 AT1G76160 83.932 529 80 2 14 542 18 541 0.0 924
MS.gene88984.t1 AT1G41830 80.669 538 98 2 5 542 11 542 0.0 907
MS.gene88984.t1 AT1G21850 77.757 526 115 2 14 539 15 538 0.0 856
MS.gene88984.t1 AT1G21860 73.535 529 137 3 10 538 12 537 0.0 823
MS.gene88984.t1 AT4G22010 71.053 532 153 1 12 542 10 541 0.0 791
MS.gene88984.t1 AT4G38420 67.523 545 171 3 3 541 5 549 0.0 755
MS.gene88984.t1 AT4G28090 62.268 538 187 7 12 538 9 541 0.0 687
MS.gene88984.t1 AT5G66920 58.880 518 211 1 21 538 30 545 0.0 646
MS.gene88984.t1 AT4G38420 65.828 477 157 3 3 473 5 481 0.0 634
MS.gene88984.t1 AT1G55570 56.044 546 227 5 1 537 5 546 0.0 602
MS.gene88984.t1 AT4G38420 66.000 450 147 3 3 446 5 454 0.0 597
MS.gene88984.t1 AT3G13390 57.663 522 211 4 24 536 24 544 0.0 586
MS.gene88984.t1 AT3G13400 54.647 538 235 4 7 536 6 542 0.0 582
MS.gene88984.t1 AT3G13400 54.647 538 235 4 7 536 6 542 0.0 582
MS.gene88984.t1 AT1G55560 53.903 538 239 4 7 536 5 541 0.0 578
MS.gene88984.t1 AT4G37160 54.614 531 234 4 14 542 16 541 0.0 577
MS.gene88984.t1 AT2G23630 55.492 528 232 2 13 540 20 544 0.0 575
MS.gene88984.t1 AT2G23630 55.492 528 232 2 13 540 15 539 0.0 574
MS.gene88984.t1 AT5G48450 51.386 541 249 9 7 535 8 546 0.0 544
MS.gene88984.t1 AT5G48450 51.386 541 249 9 7 535 8 546 0.0 543
MS.gene88984.t1 AT4G12420 50.725 552 248 8 1 532 1 548 0.0 530
MS.gene88984.t1 AT4G12420 50.725 552 248 8 1 532 1 548 0.0 530
MS.gene88984.t1 AT5G51480 49.811 530 245 8 23 532 23 551 0.0 522
MS.gene88984.t1 AT1G75790 50.277 541 253 5 10 534 4 544 2.09e-180 519
MS.gene88984.t1 AT4G25240 49.819 552 256 8 1 532 1 551 7.63e-177 511
MS.gene88984.t1 AT4G25240 49.819 552 256 8 1 532 1 551 7.63e-177 511
MS.gene88984.t1 AT4G37160 54.943 435 193 3 14 446 16 449 1.53e-165 478
MS.gene88984.t1 AT4G39830 27.698 556 336 14 2 504 13 555 1.91e-56 199
MS.gene88984.t1 AT5G21105 27.241 580 338 18 12 532 22 576 2.87e-54 193
MS.gene88984.t1 AT5G21105 27.933 537 308 17 12 489 22 538 6.68e-52 186
MS.gene88984.t1 AT5G58910 28.598 535 329 16 7 497 8 533 9.23e-52 186
MS.gene88984.t1 AT2G38080 27.172 541 358 13 23 531 22 558 3.93e-51 184
MS.gene88984.t1 AT5G21100 27.143 560 323 19 32 532 28 561 9.52e-51 183
MS.gene88984.t1 AT5G58910 28.598 535 328 17 7 497 8 532 4.02e-50 181
MS.gene88984.t1 AT5G03260 27.465 568 355 17 5 531 6 557 1.81e-49 179
MS.gene88984.t1 AT5G01190 27.360 519 337 12 28 511 25 538 3.83e-47 173
MS.gene88984.t1 AT5G01190 27.220 518 337 12 49 531 32 544 1.34e-45 168
MS.gene88984.t1 AT5G58910 29.268 492 289 16 49 497 14 489 1.83e-44 165
MS.gene88984.t1 AT4G39830 27.549 461 272 12 96 504 8 458 1.51e-40 153
MS.gene88984.t1 AT4G39830 27.549 461 272 12 96 504 10 460 1.79e-40 153
MS.gene88984.t1 AT5G05390 27.054 499 313 14 42 497 41 531 9.71e-39 149
MS.gene88984.t1 AT2G29130 26.151 543 344 17 2 496 5 538 2.01e-38 149
MS.gene88984.t1 AT2G30210 26.908 498 309 16 46 496 46 535 2.46e-38 148
MS.gene88984.t1 AT2G46570 23.757 543 357 14 28 525 32 562 3.80e-38 148
MS.gene88984.t1 AT3G09220 26.554 531 339 15 1 491 8 527 3.93e-37 145
MS.gene88984.t1 AT5G48100 26.600 500 319 16 49 505 43 537 4.15e-36 142
MS.gene88984.t1 AT4G39830 26.681 461 263 12 96 504 10 447 8.17e-36 140
MS.gene88984.t1 AT5G09360 26.136 528 344 16 7 496 15 534 1.91e-35 140
MS.gene88984.t1 AT1G18140 24.762 525 334 17 28 497 29 547 1.99e-35 140
MS.gene88984.t1 AT5G21105 27.720 386 231 12 173 532 22 385 1.17e-34 135
MS.gene88984.t1 AT5G01040 27.038 503 302 17 39 491 39 526 2.11e-34 137
MS.gene88984.t1 AT5G01050 25.967 543 343 17 39 533 39 570 2.80e-34 137
MS.gene88984.t1 AT2G40370 23.680 587 385 15 1 531 1 580 4.53e-34 136
MS.gene88984.t1 AT5G07130 24.774 553 334 18 14 504 10 542 2.83e-33 134
MS.gene88984.t1 AT2G46570 24.017 483 316 15 85 525 4 477 4.58e-29 120
MS.gene88984.t1 AT3G09220 25.814 430 278 11 101 491 3 430 1.75e-25 110
MS.gene88984.t1 AT5G60020 25.538 325 207 7 1 306 1 309 3.16e-24 107
MS.gene88984.t1 AT5G60020 25.538 325 207 7 1 306 1 309 3.74e-24 106
MS.gene88984.t1 AT5G60020 26.199 271 180 5 49 306 14 277 5.17e-23 103
MS.gene88984.t1 AT5G07130 24.580 476 277 18 91 504 2 457 1.79e-22 101
MS.gene88984.t1 AT5G07130 24.580 476 277 18 91 504 2 457 1.79e-22 101

Find 165 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGGATGTCTTCATCATTT+TGG 0.141546 1.4:+417223 None:intergenic
GGAACTTGAGGTCAGAATTT+TGG 0.153063 1.4:-416947 MS.gene88984:CDS
CACAAAGCTGCTGGTGGTTT+TGG 0.164848 1.4:-418289 MS.gene88984:CDS
TGCTGTCAGGTTTGTCCTAA+TGG 0.243582 1.4:+417533 None:intergenic
AACTCTGCAGGTCCAGTTTC+AGG 0.260810 1.4:-417612 MS.gene88984:CDS
ATGTTACAGGAAAGACTTAC+AGG 0.284854 1.4:-417881 MS.gene88984:intron
GGCTACTCTTTCTTTGTTGT+TGG 0.288891 1.4:-417183 MS.gene88984:CDS
TTCTATTATTTCCCATCTCT+TGG 0.289041 1.4:-418316 MS.gene88984:CDS
AGTTCAGCTGGACAAGTTAA+TGG 0.296097 1.4:-417435 MS.gene88984:CDS
CCACAAAGTAATGCATTCTT+AGG 0.312465 1.4:+416846 None:intergenic
GACAATGGACAAGTGATAAA+AGG 0.313235 1.4:-417070 MS.gene88984:CDS
CGTATGAGTTCCTTCTTTGC+TGG 0.315087 1.4:+418425 None:intergenic
GGTGATTACACTGTCCTTAT+TGG 0.319377 1.4:-418211 MS.gene88984:CDS
GGTTCAAGGACCAATCAATT+TGG 0.323811 1.4:+417565 None:intergenic
AGTAATGCATTCTTAGGAAT+AGG 0.325123 1.4:+416852 None:intergenic
GGTCTATCAGAGATGCTTCC+AGG 0.328166 1.4:+417318 None:intergenic
TTACTTTGTGGTAGGGCAAC+TGG 0.340111 1.4:-416834 MS.gene88984:CDS
CCGCAAGGATCATACCATTA+TGG 0.358471 1.4:-417492 MS.gene88984:CDS
GATCCTTGCGGGTTTGGTCT+TGG 0.363379 1.4:+417504 None:intergenic
GCTACTCTTTCTTTGTTGTT+GGG 0.364251 1.4:-417182 MS.gene88984:intron
TCGAAGGGAACTGGAATTCT+TGG 0.371772 1.4:+418244 None:intergenic
CCTAAGAATGCATTACTTTG+TGG 0.376644 1.4:-416846 MS.gene88984:CDS
CTTATCAATGGTCGTGGGTC+TGG 0.377274 1.4:-418036 MS.gene88984:CDS
AAGAAGTCAGCCAGCTTAAG+AGG 0.378912 1.4:+417363 None:intergenic
TCCACTTGGTGTTCGCCAAC+AGG 0.379493 1.4:-419427 MS.gene88984:intron
GTCTTGGTCCACTTGCTGTC+AGG 0.380810 1.4:+417520 None:intergenic
CATAATGGTATGATCCTTGC+GGG 0.382551 1.4:+417493 None:intergenic
CTTCAGAGCTATCATCTTGA+TGG 0.384388 1.4:-417204 MS.gene88984:CDS
TGAACCAAGCCCGCTCCATT+AGG 0.386633 1.4:-417548 MS.gene88984:CDS
CTTCAAGTGAAAGATCAAAT+TGG 0.388160 1.4:-418343 MS.gene88984:CDS
TGGTATGATCCTTGCGGGTT+TGG 0.392790 1.4:+417498 None:intergenic
CAAAACCACCAGCAGCTTTG+TGG 0.393298 1.4:+418290 None:intergenic
ACACTGTCCTTATTGGTGAT+TGG 0.395975 1.4:-418204 MS.gene88984:CDS
TCATACGAAGATGGAGTGTT+TGG 0.398452 1.4:-418409 MS.gene88984:CDS
GTTGCTGTAGCGAAGAATTC+CGG 0.398646 1.4:+417632 None:intergenic
CTTGTTTCCGTTATCAAGTT+GGG 0.404501 1.4:+418080 None:intergenic
ACTTGTACCAATCACCAATA+AGG 0.411862 1.4:+418197 None:intergenic
ATGTATGTAAAGTTCTTTCC+AGG 0.413929 1.4:+418367 None:intergenic
ATCCATACAGGTTCTTCAAC+TGG 0.414214 1.4:-419473 MS.gene88984:CDS
GTAATGCATTCTTAGGAATA+GGG 0.417767 1.4:+416853 None:intergenic
ATTTGGATGGTGGGTCCACC+AGG 0.418556 1.4:+417582 None:intergenic
TTGTCCTAATGGAGCGGGCT+TGG 0.419822 1.4:+417544 None:intergenic
TTAAGTTTATCATGCCATAA+TGG 0.419861 1.4:+417478 None:intergenic
GAATGTCTGGTCCTGGGAAT+TGG 0.420914 1.4:+419266 None:intergenic
AATGGAGCGGGCTTGGTTCA+AGG 0.421159 1.4:+417551 None:intergenic
CCTGATGGAATTCTTATCAA+TGG 0.421680 1.4:-418048 MS.gene88984:CDS
GCGGGATCATCGAAGGGAAC+TGG 0.422110 1.4:+418235 None:intergenic
GCCAGCTTAAGAGGAGTATC+GGG 0.437831 1.4:+417372 None:intergenic
AAGTTCTTTCCAGGTGGAAT+GGG 0.445944 1.4:+418376 None:intergenic
GTTCAGCTGGACAAGTTAAT+GGG 0.447873 1.4:-417434 MS.gene88984:CDS
CAAGGGAGGAGTAGGTGGTT+TGG 0.449186 1.4:+417763 None:intergenic
AGCCAGCTTAAGAGGAGTAT+CGG 0.452171 1.4:+417371 None:intergenic
CTGGAATTCTTGGCGTACTT+AGG 0.454106 1.4:+418254 None:intergenic
TAGATAGCAGTCCATGACTT+TGG 0.469788 1.4:+416996 None:intergenic
TATTATGTGACAGGATTTGA+AGG 0.472072 1.4:-418103 MS.gene88984:intron
TGGATGGTGGGTCCACCAGG+GGG 0.473394 1.4:+417585 None:intergenic
GACCCACCATCCAAATTGAT+TGG 0.477439 1.4:-417575 MS.gene88984:CDS
CAGTGAAATGGCACTGAACT+TGG 0.480159 1.4:-419553 None:intergenic
CTTGGATTCCACAAAGCTGC+TGG 0.481877 1.4:-418298 MS.gene88984:CDS
TTTGTCACAGAATGAATGTC+TGG 0.484656 1.4:+419253 None:intergenic
CAGGTTTGTCCTAATGGAGC+GGG 0.488484 1.4:+417539 None:intergenic
AAAGTTCTTTCCAGGTGGAA+TGG 0.489538 1.4:+418375 None:intergenic
TTGAAGGCCCAACTTGATAA+CGG 0.491854 1.4:-418087 MS.gene88984:CDS
CCCCGATACTCCTCTTAAGC+TGG 0.492307 1.4:-417373 MS.gene88984:CDS
CCATTGATAAGAATTCCATC+AGG 0.493362 1.4:+418048 None:intergenic
ACTTGTTTCCGTTATCAAGT+TGG 0.493576 1.4:+418079 None:intergenic
ATTCCGGTGGAGGTGAGTAC+AGG 0.496771 1.4:+417648 None:intergenic
CTCCTCCCTTGACGTTCACG+TGG 0.496861 1.4:-417751 MS.gene88984:CDS
ACAGAATGAATGTCTGGTCC+TGG 0.504301 1.4:+419259 None:intergenic
ATAGCAGGTGTTTACCGTCC+TGG 0.506103 1.4:-417336 MS.gene88984:CDS
ACTAACCATGAAATGAGAAA+AGG 0.507335 1.4:+419190 None:intergenic
GGAATGGAGTCCAGCAAAGA+AGG 0.507749 1.4:-418435 MS.gene88984:CDS
TTCCAGTTGAAGAACCTGTA+TGG 0.507790 1.4:+419471 None:intergenic
GGGAGGAGTAGGTGGTTTGG+AGG 0.508210 1.4:+417766 None:intergenic
TATCAATGTCTTCAACAGCT+TGG 0.512023 1.4:-419218 MS.gene88984:CDS
GGAATTCTTATCAATGGTCG+TGG 0.513900 1.4:-418042 MS.gene88984:CDS
GTAGCTAAGAAGTCATCAAA+GGG 0.515250 1.4:+416797 None:intergenic
AGAATGCATTACTTTGTGGT+AGG 0.515449 1.4:-416842 MS.gene88984:CDS
CTGCGTCTCTAAGATTGTAC+TGG 0.516471 1.4:+417045 None:intergenic
TTGGATGGTGGGTCCACCAG+GGG 0.516542 1.4:+417584 None:intergenic
CAAGATGAAGCTGGTAGAAG+TGG 0.521035 1.4:-417802 MS.gene88984:CDS
AACAAGTTACCTTTCCCTGA+TGG 0.521178 1.4:-418063 MS.gene88984:CDS
GCTGACTTCTTCAAGATAGC+AGG 0.524240 1.4:-417351 MS.gene88984:CDS
TAGAATATCAAATGTGGGAT+CGG 0.526824 1.4:-417856 MS.gene88984:CDS
TTCAACTGGAACGTTACCTA+TGG 0.528833 1.4:-419459 MS.gene88984:CDS
TTTGGATGGTGGGTCCACCA+GGG 0.529837 1.4:+417583 None:intergenic
TTGTTGCTGAAGATCCATAC+AGG 0.530698 1.4:-419485 MS.gene88984:CDS
GAATGCATTACTTTGTGGTA+GGG 0.534147 1.4:-416841 MS.gene88984:CDS
GGATTCCACAAAGCTGCTGG+TGG 0.534304 1.4:-418295 MS.gene88984:CDS
GGAGGAGTAGGTGGTTTGGA+GGG 0.534506 1.4:+417767 None:intergenic
ATCAATGGTCGTGGGTCTGG+AGG 0.535103 1.4:-418033 MS.gene88984:CDS
CTTATCAATGGCCAATTCCC+AGG 0.536067 1.4:-419277 MS.gene88984:CDS
CTTCGCTACAGCAACTCTGC+AGG 0.536476 1.4:-417624 MS.gene88984:CDS
ATGATTAGTTACCGTGTGGT+TGG 0.536620 1.4:+418174 None:intergenic
TTTGGGCACGACAATATCTC+GGG 0.539491 1.4:-416929 MS.gene88984:CDS
TATGGTGACATTTATCCACT+TGG 0.540771 1.4:-419441 MS.gene88984:CDS
CATCAAGATGATAGCTCTGA+AGG 0.545903 1.4:+417205 None:intergenic
TATGTTGCACTTGACAATGT+AGG 0.546497 1.4:-416975 MS.gene88984:CDS
TCTGATAGACCTAGTGGTGG+TGG 0.549192 1.4:-417306 MS.gene88984:CDS
AATCCTGTACTCACCTCCAC+CGG 0.553249 1.4:-417651 MS.gene88984:CDS
ATCACCAGCGGGATCATCGA+AGG 0.554567 1.4:+418228 None:intergenic
CAGGCTTAGAATATCAAATG+TGG 0.556229 1.4:-417862 MS.gene88984:CDS
ATTGTGCTATCAAGTTCAGC+TGG 0.557942 1.4:-417447 MS.gene88984:CDS
AGAAGGAACTCATACGAAGA+TGG 0.560891 1.4:-418418 MS.gene88984:CDS
GACCAATCAATTTGGATGGT+GGG 0.562075 1.4:+417573 None:intergenic
AGCAACAATAGTAGTAGTGA+TGG 0.562486 1.4:+419500 None:intergenic
TTGCAGGGAATACTTATCAA+TGG 0.563989 1.4:-419289 MS.gene88984:intron
CAGAATGAATGTCTGGTCCT+GGG 0.565547 1.4:+419260 None:intergenic
GCTTTGTGGAATCCAAGAGA+TGG 0.565601 1.4:+418304 None:intergenic
CAAGGACCAATCAATTTGGA+TGG 0.569010 1.4:+417569 None:intergenic
AGGACAAACCTGACAGCAAG+TGG 0.574032 1.4:-417528 MS.gene88984:CDS
GTGAACGTCAAGGGAGGAGT+AGG 0.576942 1.4:+417755 None:intergenic
CTAGTAACCGCGGATCAACC+TGG 0.581592 1.4:-417711 MS.gene88984:CDS
GGACCAATCAATTTGGATGG+TGG 0.582450 1.4:+417572 None:intergenic
CTCAGGTATATCCAAAGTCA+TGG 0.583355 1.4:-417007 MS.gene88984:CDS
GGTGGAGGTGAGTACAGGAT+TGG 0.585429 1.4:+417653 None:intergenic
GTAGCGAAGAATTCCGGTGG+AGG 0.585607 1.4:+417638 None:intergenic
ACAACATGTCCCATTCCACC+TGG 0.586121 1.4:-418385 MS.gene88984:CDS
CTTTGTGGAATCCAAGAGAT+GGG 0.586569 1.4:+418305 None:intergenic
ACCTGTTGGCGAACACCAAG+TGG 0.587332 1.4:+419426 None:intergenic
GTTCCCTTCGATGATCCCGC+TGG 0.588900 1.4:-418232 MS.gene88984:CDS
ATGTAGGAATGTGGAACTTG+AGG 0.589636 1.4:-416959 MS.gene88984:CDS
ACAGAATCACAAGATGAAGC+TGG 0.591529 1.4:-417811 MS.gene88984:CDS
TTGACCCACGTGAACGTCAA+GGG 0.599164 1.4:+417746 None:intergenic
AATGTTGCATGCAGTATGGA+TGG 0.602475 1.4:-417095 MS.gene88984:intron
ATAAGAATTCCATCAGGGAA+AGG 0.603702 1.4:+418054 None:intergenic
TCAGGTTTGTCCTAATGGAG+CGG 0.604135 1.4:+417538 None:intergenic
AAGTGGATAAATGTCACCAT+AGG 0.607359 1.4:+419443 None:intergenic
AGGACAGTGTAATCACCAGC+GGG 0.611484 1.4:+418217 None:intergenic
CCATAATGGTATGATCCTTG+CGG 0.613798 1.4:+417492 None:intergenic
TACAGGATTGGTAAATCGAG+AGG 0.614756 1.4:+417665 None:intergenic
AACGTCAAGGGAGGAGTAGG+TGG 0.614850 1.4:+417758 None:intergenic
TCATCTCTCAATGTGGAGCA+AGG 0.615730 1.4:-418009 MS.gene88984:intron
AGATAAATACCACCACCACT+AGG 0.622790 1.4:+417297 None:intergenic
AGGCTTAGAATATCAAATGT+GGG 0.626320 1.4:-417861 MS.gene88984:CDS
ATTGACCCACGTGAACGTCA+AGG 0.633112 1.4:+417745 None:intergenic
TAGTAGTAGTGATGGCAAAG+AGG 0.634339 1.4:+419508 None:intergenic
GCAGTATGGATGGAGGACAA+TGG 0.636774 1.4:-417085 MS.gene88984:intron
ATGAAGCTGGTAGAAGTGGA+AGG 0.637021 1.4:-417798 MS.gene88984:CDS
TCCTCCCTTGACGTTCACGT+GGG 0.641683 1.4:-417750 MS.gene88984:CDS
ATCTCTGATAGACCTAGTGG+TGG 0.645124 1.4:-417309 MS.gene88984:CDS
TATGTAAAGTTCTTTCCAGG+TGG 0.645224 1.4:+418370 None:intergenic
GAATTCTTATCAATGGTCGT+GGG 0.645558 1.4:-418041 MS.gene88984:CDS
AGTTTCGCGTTCCACCACTC+AGG 0.647895 1.4:-417024 MS.gene88984:CDS
GGACCAAGACCAAACCCGCA+AGG 0.650703 1.4:-417507 MS.gene88984:CDS
TCACCAGCGGGATCATCGAA+GGG 0.652297 1.4:+418229 None:intergenic
CACTTGACAATGTAGGAATG+TGG 0.655047 1.4:-416968 MS.gene88984:CDS
CATTGATAAGAATTCCATCA+GGG 0.656365 1.4:+418049 None:intergenic
TATCAGAGATGCTTCCAGGA+CGG 0.660561 1.4:+417322 None:intergenic
GTCTTGACCAGGTTGATCCG+CGG 0.661746 1.4:+417704 None:intergenic
ACAATATAATAGTCTTGACC+AGG 0.663963 1.4:+417693 None:intergenic
TGGAGGATCATCTCTCAATG+TGG 0.668048 1.4:-418016 MS.gene88984:CDS
AGCATCTCTGATAGACCTAG+TGG 0.673066 1.4:-417312 MS.gene88984:CDS
TTGGTACAAGTCCAACCACA+CGG 0.685221 1.4:-418185 MS.gene88984:intron
CCAGCTTAAGAGGAGTATCG+GGG 0.687155 1.4:+417373 None:intergenic
ACCCACGTGAACGTCAAGGG+AGG 0.690184 1.4:+417749 None:intergenic
AAGGACAGTGTAATCACCAG+CGG 0.694966 1.4:+418216 None:intergenic
GCTGTAGCGAAGAATTCCGG+TGG 0.700350 1.4:+417635 None:intergenic
CTTTGGATATACCTGAGTGG+TGG 0.703031 1.4:+417013 None:intergenic
ATACTCAGTTCTAGTAACCG+CGG 0.703123 1.4:-417721 MS.gene88984:CDS
CAGCTTAAGAGGAGTATCGG+GGG 0.703277 1.4:+417374 None:intergenic
GTTGCATGCAGTATGGATGG+AGG 0.723579 1.4:-417092 MS.gene88984:intron
ATGGCATGATAAACTTAACG+AGG 0.737321 1.4:-417473 MS.gene88984:CDS
TAGCTAAGAAGTCATCAAAG+GGG 0.765054 1.4:+416798 None:intergenic
TGACTTTGGATATACCTGAG+TGG 0.778186 1.4:+417010 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACATAACAAATTAACTT+TGG + chr1.4:417040-417059 None:intergenic 15.0%
!!! CACTTTTATATTCATTTATA+TGG - chr1.4:417551-417570 MS.gene88984:CDS 15.0%
!! AAAGCATGAATAAAGTAAAT+GGG + chr1.4:417720-417739 None:intergenic 20.0%
!! AATGAAAATGATCTATATGA+TGG + chr1.4:419215-419234 None:intergenic 20.0%
!! CACAATAATTTAAAAAGTAG+TGG + chr1.4:417818-417837 None:intergenic 20.0%
!! TATATTCATTTATATGGAGA+TGG - chr1.4:417557-417576 MS.gene88984:CDS 20.0%
!! TCAATTCCAAATTAAGTAAT+TGG - chr1.4:417608-417627 MS.gene88984:CDS 20.0%
!! TCATTTATTAATCAATTGCA+AGG + chr1.4:417875-417894 None:intergenic 20.0%
!! TGAAATATAAATATACCTGT+TGG + chr1.4:416949-416968 None:intergenic 20.0%
!!! TTGACACAACTAATATTTTT+TGG - chr1.4:418442-418461 MS.gene88984:intron 20.0%
! AAAATGATTCAGATCCAATA+AGG + chr1.4:416986-417005 None:intergenic 25.0%
! AAGCATGAATAAAGTAAATG+GGG + chr1.4:417719-417738 None:intergenic 25.0%
! ACGTGAAATAGTAGTATATT+AGG - chr1.4:417790-417809 MS.gene88984:CDS 25.0%
! AGATAATTAGCTCAATTTGA+TGG + chr1.4:417762-417781 None:intergenic 25.0%
! ATTGTATTGTATTATGTGAC+AGG - chr1.4:418246-418265 MS.gene88984:CDS 25.0%
! CAAAGCATGAATAAAGTAAA+TGG + chr1.4:417721-417740 None:intergenic 25.0%
! CAAATTTAGTACTAATGTTG+AGG + chr1.4:418425-418444 None:intergenic 25.0%
! CGTGAAATAGTAGTATATTA+GGG - chr1.4:417791-417810 MS.gene88984:CDS 25.0%
! GACTCATATAATGTTTCATT+TGG - chr1.4:418395-418414 MS.gene88984:CDS 25.0%
! GATGAAAAAAGAAATGTGAA+AGG + chr1.4:417199-417218 None:intergenic 25.0%
! TTAAGTTTATCATGCCATAA+TGG + chr1.4:418883-418902 None:intergenic 25.0%
!! TATAGTATGTGTGTTTTGTA+AGG + chr1.4:417462-417481 None:intergenic 25.0%
!! TGTTATTTACACATAGATAG+AGG + chr1.4:417230-417249 None:intergenic 25.0%
!!! CCTCAAAAGTCTTTTTAATT+GGG - chr1.4:417689-417708 MS.gene88984:CDS 25.0%
!!! TATATGAGTCTTATTCATGT+AGG + chr1.4:418385-418404 None:intergenic 25.0%
ACATGACATAAAAACAGGAA+TGG - chr1.4:417907-417926 MS.gene88984:intron 30.0%
ACATGACATGACATAAAAAC+AGG - chr1.4:417902-417921 MS.gene88984:intron 30.0%
ACTAACCATGAAATGAGAAA+AGG + chr1.4:417171-417190 None:intergenic 30.0%
ACTACTATTTCACGTAATCA+AGG + chr1.4:417785-417804 None:intergenic 30.0%
AGGCTTAGAATATCAAATGT+GGG - chr1.4:418497-418516 MS.gene88984:intron 30.0%
AGTAATGCATTCTTAGGAAT+AGG + chr1.4:419509-419528 None:intergenic 30.0%
ATATGAAGATATCAAACGTG+TGG + chr1.4:417332-417351 None:intergenic 30.0%
ATGTATGTAAAGTTCTTTCC+AGG + chr1.4:417994-418013 None:intergenic 30.0%
CATTGATAAGAATTCCATCA+GGG + chr1.4:418312-418331 None:intergenic 30.0%
CTTCAAGTGAAAGATCAAAT+TGG - chr1.4:418015-418034 MS.gene88984:CDS 30.0%
CTTTCATCCTTATACCTTAT+TGG - chr1.4:416969-416988 MS.gene88984:CDS 30.0%
GTAATGCATTCTTAGGAATA+GGG + chr1.4:419508-419527 None:intergenic 30.0%
TAGAATATCAAATGTGGGAT+CGG - chr1.4:418502-418521 MS.gene88984:intron 30.0%
TATTATGTGACAGGATTTGA+AGG - chr1.4:418255-418274 MS.gene88984:CDS 30.0%
TTCAGATCCAATAAGGTATA+AGG + chr1.4:416979-416998 None:intergenic 30.0%
TTCTATTATTTCCCATCTCT+TGG - chr1.4:418042-418061 MS.gene88984:CDS 30.0%
! ACAATATAATAGTCTTGACC+AGG + chr1.4:418668-418687 None:intergenic 30.0%
! ACCTAGTACTACTACATTTT+GGG - chr1.4:417367-417386 MS.gene88984:CDS 30.0%
! CCCAATTAAAAAGACTTTTG+AGG + chr1.4:417692-417711 None:intergenic 30.0%
! GCCTCAAAAGTCTTTTTAAT+TGG - chr1.4:417688-417707 MS.gene88984:CDS 30.0%
! TACCTAGTACTACTACATTT+TGG - chr1.4:417366-417385 MS.gene88984:CDS 30.0%
!! CAAAAGTCTTTTTAATTGGG+TGG - chr1.4:417692-417711 MS.gene88984:CDS 30.0%
!!! ATTAAAAAGACTTTTGAGGC+AGG + chr1.4:417688-417707 None:intergenic 30.0%
!!! ATTTTGGGATGTTTGTTTGT+TGG - chr1.4:417382-417401 MS.gene88984:CDS 30.0%
!!! TTTTGGGATGTTTGTTTGTT+GGG - chr1.4:417383-417402 MS.gene88984:CDS 30.0%
AAGTGGATAAATGTCACCAT+AGG + chr1.4:416918-416937 None:intergenic 35.0%
ACTTGTACCAATCACCAATA+AGG + chr1.4:418164-418183 None:intergenic 35.0%
ACTTGTTTCCGTTATCAAGT+TGG + chr1.4:418282-418301 None:intergenic 35.0%
AGAATGCATTACTTTGTGGT+AGG - chr1.4:419516-419535 MS.gene88984:CDS 35.0%
AGCAACAATAGTAGTAGTGA+TGG + chr1.4:416861-416880 None:intergenic 35.0%
ATAAGAATTCCATCAGGGAA+AGG + chr1.4:418307-418326 None:intergenic 35.0%
ATGGCATGATAAACTTAACG+AGG - chr1.4:418885-418904 MS.gene88984:intron 35.0%
ATGTTACAGGAAAGACTTAC+AGG - chr1.4:418477-418496 MS.gene88984:intron 35.0%
CAGGCTTAGAATATCAAATG+TGG - chr1.4:418496-418515 MS.gene88984:intron 35.0%
CCACAAAGTAATGCATTCTT+AGG + chr1.4:419515-419534 None:intergenic 35.0%
CCATTGATAAGAATTCCATC+AGG + chr1.4:418313-418332 None:intergenic 35.0%
CCTAAGAATGCATTACTTTG+TGG - chr1.4:419512-419531 MS.gene88984:CDS 35.0%
CCTGATGGAATTCTTATCAA+TGG - chr1.4:418310-418329 MS.gene88984:CDS 35.0%
CTTGTTTCCGTTATCAAGTT+GGG + chr1.4:418281-418300 None:intergenic 35.0%
GAATGCATTACTTTGTGGTA+GGG - chr1.4:419517-419536 MS.gene88984:CDS 35.0%
GAATTCTTATCAATGGTCGT+GGG - chr1.4:418317-418336 MS.gene88984:CDS 35.0%
GACAATGGACAAGTGATAAA+AGG - chr1.4:419288-419307 MS.gene88984:intron 35.0%
GCATGTTATGTTGTTAGAGT+TGG - chr1.4:417269-417288 MS.gene88984:CDS 35.0%
TATCAATGTCTTCAACAGCT+TGG - chr1.4:417140-417159 MS.gene88984:intron 35.0%
TATCTCTTGTAGTACTATGC+TGG - chr1.4:417488-417507 MS.gene88984:CDS 35.0%
TATGAATGTTGCATGCAGTA+TGG - chr1.4:419259-419278 MS.gene88984:CDS 35.0%
TATGGTGACATTTATCCACT+TGG - chr1.4:416917-416936 MS.gene88984:CDS 35.0%
TATGTAAAGTTCTTTCCAGG+TGG + chr1.4:417991-418010 None:intergenic 35.0%
TATGTTGCACTTGACAATGT+AGG - chr1.4:419383-419402 MS.gene88984:intron 35.0%
TCCCAAAATGTAGTAGTACT+AGG + chr1.4:417371-417390 None:intergenic 35.0%
TCCTGTAACATCAATGACAA+GGG + chr1.4:418468-418487 None:intergenic 35.0%
TGCTGGCCAATTACTTAATT+TGG + chr1.4:417617-417636 None:intergenic 35.0%
TTCCTGTAACATCAATGACA+AGG + chr1.4:418469-418488 None:intergenic 35.0%
TTGCAGGGAATACTTATCAA+TGG - chr1.4:417069-417088 MS.gene88984:CDS 35.0%
TTTACACATAGATAGAGGCT+AGG + chr1.4:417225-417244 None:intergenic 35.0%
TTTGTCACAGAATGAATGTC+TGG + chr1.4:417108-417127 None:intergenic 35.0%
! ACGAACCTTTTCTCATTTCA+TGG - chr1.4:417163-417182 MS.gene88984:intron 35.0%
! ATGTTTTGTGACAAACTTGC+AGG - chr1.4:417053-417072 MS.gene88984:CDS 35.0%
! GAACTTGAGGTCAGAATTTT+GGG - chr1.4:419412-419431 MS.gene88984:intron 35.0%
! GAAGGATGTCTTCATCATTT+TGG + chr1.4:419138-419157 None:intergenic 35.0%
! GTAAATGATTAGTTACCGTG+TGG + chr1.4:418191-418210 None:intergenic 35.0%
! TCAATTTGATGGTTAGCTAG+AGG + chr1.4:417751-417770 None:intergenic 35.0%
! TGTTTTGTGACAAACTTGCA+GGG - chr1.4:417054-417073 MS.gene88984:CDS 35.0%
!! GCTACTCTTTCTTTGTTGTT+GGG - chr1.4:419176-419195 MS.gene88984:intron 35.0%
AAAAGTGAAGCTAGCAGATC+TGG + chr1.4:417538-417557 None:intergenic 40.0%
AAAGTGAAGCTAGCAGATCT+GGG + chr1.4:417537-417556 None:intergenic 40.0%
AAAGTTCTTTCCAGGTGGAA+TGG + chr1.4:417986-418005 None:intergenic 40.0%
AACAAGTTACCTTTCCCTGA+TGG - chr1.4:418295-418314 MS.gene88984:CDS 40.0%
AAGTTCTTTCCAGGTGGAAT+GGG + chr1.4:417985-418004 None:intergenic 40.0%
AATGTTGCATGCAGTATGGA+TGG - chr1.4:419263-419282 MS.gene88984:CDS 40.0%
ACAGAATCACAAGATGAAGC+TGG - chr1.4:418547-418566 MS.gene88984:intron 40.0%
ACCACTTGTATTGTGTTGTG+TGG - chr1.4:417410-417429 MS.gene88984:CDS 40.0%
AGAAGGAACTCATACGAAGA+TGG - chr1.4:417940-417959 MS.gene88984:intron 40.0%
AGATAAATACCACCACCACT+AGG + chr1.4:419064-419083 None:intergenic 40.0%
AGTTCAGCTGGACAAGTTAA+TGG - chr1.4:418923-418942 MS.gene88984:intron 40.0%
ATCCATACAGGTTCTTCAAC+TGG - chr1.4:416885-416904 MS.gene88984:CDS 40.0%
ATGTAGGAATGTGGAACTTG+AGG - chr1.4:419399-419418 MS.gene88984:intron 40.0%
ATTGTGCTATCAAGTTCAGC+TGG - chr1.4:418911-418930 MS.gene88984:intron 40.0%
CAAGGACCAATCAATTTGGA+TGG + chr1.4:418792-418811 None:intergenic 40.0%
CACTTGACAATGTAGGAATG+TGG - chr1.4:419390-419409 MS.gene88984:intron 40.0%
CATAATGGTATGATCCTTGC+GGG + chr1.4:418868-418887 None:intergenic 40.0%
CCATAATGGTATGATCCTTG+CGG + chr1.4:418869-418888 None:intergenic 40.0%
CTCAGGTATATCCAAAGTCA+TGG - chr1.4:419351-419370 MS.gene88984:intron 40.0%
CTTCAGAGCTATCATCTTGA+TGG - chr1.4:419154-419173 MS.gene88984:intron 40.0%
CTTTGTGGAATCCAAGAGAT+GGG + chr1.4:418056-418075 None:intergenic 40.0%
GACCAATCAATTTGGATGGT+GGG + chr1.4:418788-418807 None:intergenic 40.0%
GGAATTCTTATCAATGGTCG+TGG - chr1.4:418316-418335 MS.gene88984:CDS 40.0%
GGTGATTACACTGTCCTTAT+TGG - chr1.4:418147-418166 MS.gene88984:intron 40.0%
GGTTCAAGGACCAATCAATT+TGG + chr1.4:418796-418815 None:intergenic 40.0%
GTTCAGCTGGACAAGTTAAT+GGG - chr1.4:418924-418943 MS.gene88984:intron 40.0%
TAGATAGCAGTCCATGACTT+TGG + chr1.4:419365-419384 None:intergenic 40.0%
TAGTAGTAGTGATGGCAAAG+AGG + chr1.4:416853-416872 None:intergenic 40.0%
TGACTTTGGATATACCTGAG+TGG + chr1.4:419351-419370 None:intergenic 40.0%
TTCAACTGGAACGTTACCTA+TGG - chr1.4:416899-416918 MS.gene88984:CDS 40.0%
TTCCAGTTGAAGAACCTGTA+TGG + chr1.4:416890-416909 None:intergenic 40.0%
TTGAAGGCCCAACTTGATAA+CGG - chr1.4:418271-418290 MS.gene88984:CDS 40.0%
TTGTAGTACTATGCTGGTCA+TGG - chr1.4:417494-417513 MS.gene88984:CDS 40.0%
TTGTTGCTGAAGATCCATAC+AGG - chr1.4:416873-416892 MS.gene88984:CDS 40.0%
! ACACTGTCCTTATTGGTGAT+TGG - chr1.4:418154-418173 MS.gene88984:intron 40.0%
! ATACTCAGTTCTAGTAACCG+CGG - chr1.4:418637-418656 MS.gene88984:intron 40.0%
! ATGATTAGTTACCGTGTGGT+TGG + chr1.4:418187-418206 None:intergenic 40.0%
! GGAACTTGAGGTCAGAATTT+TGG - chr1.4:419411-419430 MS.gene88984:intron 40.0%
! GGCTACTCTTTCTTTGTTGT+TGG - chr1.4:419175-419194 MS.gene88984:intron 40.0%
! TACAGGATTGGTAAATCGAG+AGG + chr1.4:418696-418715 None:intergenic 40.0%
! TCATACGAAGATGGAGTGTT+TGG - chr1.4:417949-417968 MS.gene88984:intron 40.0%
! TGGCTAGCTAACTAACTAAC+AGG + chr1.4:417312-417331 None:intergenic 40.0%
! TTTTGGGCACGACAATATCT+CGG - chr1.4:419428-419447 MS.gene88984:CDS 40.0%
!! CATCAAGATGATAGCTCTGA+AGG + chr1.4:419156-419175 None:intergenic 40.0%
AAGAAGTCAGCCAGCTTAAG+AGG + chr1.4:418998-419017 None:intergenic 45.0%
AAGGACAGTGTAATCACCAG+CGG + chr1.4:418145-418164 None:intergenic 45.0%
AATTGGCCAGCACTGCAATT+TGG - chr1.4:417625-417644 MS.gene88984:CDS 45.0%
ACAGAATGAATGTCTGGTCC+TGG + chr1.4:417102-417121 None:intergenic 45.0%
AGAAAGCCAAATTGCAGTGC+TGG + chr1.4:417634-417653 None:intergenic 45.0%
AGCATCTCTGATAGACCTAG+TGG - chr1.4:419046-419065 MS.gene88984:intron 45.0%
AGTACTATGCTGGTCATGGT+GGG - chr1.4:417498-417517 MS.gene88984:CDS 45.0%
ATCTCTGATAGACCTAGTGG+TGG - chr1.4:419049-419068 MS.gene88984:intron 45.0%
ATGAAGCTGGTAGAAGTGGA+AGG - chr1.4:418560-418579 MS.gene88984:intron 45.0%
ATTGTGTTGTGTGGCTGCAA+AGG - chr1.4:417419-417438 MS.gene88984:CDS 45.0%
CAAGATGAAGCTGGTAGAAG+TGG - chr1.4:418556-418575 MS.gene88984:intron 45.0%
CACATAGATAGAGGCTAGGA+TGG + chr1.4:417221-417240 None:intergenic 45.0%
CAGAATGAATGTCTGGTCCT+GGG + chr1.4:417101-417120 None:intergenic 45.0%
CCGCAAGGATCATACCATTA+TGG - chr1.4:418866-418885 MS.gene88984:intron 45.0%
CGTATGAGTTCCTTCTTTGC+TGG + chr1.4:417936-417955 None:intergenic 45.0%
CTGGAATTCTTGGCGTACTT+AGG + chr1.4:418107-418126 None:intergenic 45.0%
CTTATCAATGGCCAATTCCC+AGG - chr1.4:417081-417100 MS.gene88984:CDS 45.0%
CTTTGGATATACCTGAGTGG+TGG + chr1.4:419348-419367 None:intergenic 45.0%
GACCCACCATCCAAATTGAT+TGG - chr1.4:418783-418802 MS.gene88984:intron 45.0%
GCCACACAACACAATACAAG+TGG + chr1.4:417414-417433 None:intergenic 45.0%
GCCCTTGTCATTGATGTTAC+AGG - chr1.4:418464-418483 MS.gene88984:intron 45.0%
GCTTTGTGGAATCCAAGAGA+TGG + chr1.4:418057-418076 None:intergenic 45.0%
GGACCAATCAATTTGGATGG+TGG + chr1.4:418789-418808 None:intergenic 45.0%
GTTGCTGTAGCGAAGAATTC+CGG + chr1.4:418729-418748 None:intergenic 45.0%
TAGTACTATGCTGGTCATGG+TGG - chr1.4:417497-417516 MS.gene88984:CDS 45.0%
TATCAGAGATGCTTCCAGGA+CGG + chr1.4:419039-419058 None:intergenic 45.0%
TCAGGTTTGTCCTAATGGAG+CGG + chr1.4:418823-418842 None:intergenic 45.0%
TCATCTCTCAATGTGGAGCA+AGG - chr1.4:418349-418368 MS.gene88984:CDS 45.0%
TCGAAGGGAACTGGAATTCT+TGG + chr1.4:418117-418136 None:intergenic 45.0%
TGCTGTCAGGTTTGTCCTAA+TGG + chr1.4:418828-418847 None:intergenic 45.0%
TGGAGGATCATCTCTCAATG+TGG - chr1.4:418342-418361 MS.gene88984:CDS 45.0%
TTACTTTGTGGTAGGGCAAC+TGG - chr1.4:419524-419543 MS.gene88984:CDS 45.0%
TTGGTACAAGTCCAACCACA+CGG - chr1.4:418173-418192 MS.gene88984:intron 45.0%
TTTGGGCACGACAATATCTC+GGG - chr1.4:419429-419448 MS.gene88984:CDS 45.0%
! AGCCAGCTTAAGAGGAGTAT+CGG + chr1.4:418990-419009 None:intergenic 45.0%
! GCTGACTTCTTCAAGATAGC+AGG - chr1.4:419007-419026 MS.gene88984:intron 45.0%
!! CTGCGTCTCTAAGATTGTAC+TGG + chr1.4:419316-419335 None:intergenic 45.0%
!!! AAAAGACTTTTGAGGCAGGC+AGG + chr1.4:417684-417703 None:intergenic 45.0%
AACTCTGCAGGTCCAGTTTC+AGG - chr1.4:418746-418765 MS.gene88984:intron 50.0%
AATCCTGTACTCACCTCCAC+CGG - chr1.4:418707-418726 MS.gene88984:intron 50.0%
ACAACATGTCCCATTCCACC+TGG - chr1.4:417973-417992 MS.gene88984:intron 50.0%
AGGACAAACCTGACAGCAAG+TGG - chr1.4:418830-418849 MS.gene88984:intron 50.0%
AGGACAGTGTAATCACCAGC+GGG + chr1.4:418144-418163 None:intergenic 50.0%
ATAGCAGGTGTTTACCGTCC+TGG - chr1.4:419022-419041 MS.gene88984:intron 50.0%
ATTGACCCACGTGAACGTCA+AGG + chr1.4:418616-418635 None:intergenic 50.0%
CAAAACCACCAGCAGCTTTG+TGG + chr1.4:418071-418090 None:intergenic 50.0%
CAGGTTTGTCCTAATGGAGC+GGG + chr1.4:418822-418841 None:intergenic 50.0%
CTTATCAATGGTCGTGGGTC+TGG - chr1.4:418322-418341 MS.gene88984:CDS 50.0%
CTTGGATTCCACAAAGCTGC+TGG - chr1.4:418060-418079 MS.gene88984:CDS 50.0%
GAATGTCTGGTCCTGGGAAT+TGG + chr1.4:417095-417114 None:intergenic 50.0%
GCAGTATGGATGGAGGACAA+TGG - chr1.4:419273-419292 MS.gene88984:CDS 50.0%
GGAATGGAGTCCAGCAAAGA+AGG - chr1.4:417923-417942 MS.gene88984:intron 50.0%
GGTCTATCAGAGATGCTTCC+AGG + chr1.4:419043-419062 None:intergenic 50.0%
GTTGCATGCAGTATGGATGG+AGG - chr1.4:419266-419285 MS.gene88984:CDS 50.0%
GTTGTGTGGCTGCAAAGGAT+AGG - chr1.4:417424-417443 MS.gene88984:CDS 50.0%
TCTGATAGACCTAGTGGTGG+TGG - chr1.4:419052-419071 MS.gene88984:intron 50.0%
TGGTATGATCCTTGCGGGTT+TGG + chr1.4:418863-418882 None:intergenic 50.0%
TTGACCCACGTGAACGTCAA+GGG + chr1.4:418615-418634 None:intergenic 50.0%
! CACAAAGCTGCTGGTGGTTT+TGG - chr1.4:418069-418088 MS.gene88984:CDS 50.0%
!! CAGCTTAAGAGGAGTATCGG+GGG + chr1.4:418987-419006 None:intergenic 50.0%
!! CCAGCTTAAGAGGAGTATCG+GGG + chr1.4:418988-419007 None:intergenic 50.0%
!! GCCAGCTTAAGAGGAGTATC+GGG + chr1.4:418989-419008 None:intergenic 50.0%
!!! AAAGCTGCTGGTGGTTTTGG+AGG - chr1.4:418072-418091 MS.gene88984:CDS 50.0%
AACGTCAAGGGAGGAGTAGG+TGG + chr1.4:418603-418622 None:intergenic 55.0%
ACCTGTTGGCGAACACCAAG+TGG + chr1.4:416935-416954 None:intergenic 55.0%
AGTTTCGCGTTCCACCACTC+AGG - chr1.4:419334-419353 MS.gene88984:intron 55.0%
ATCAATGGTCGTGGGTCTGG+AGG - chr1.4:418325-418344 MS.gene88984:CDS 55.0%
ATCACCAGCGGGATCATCGA+AGG + chr1.4:418133-418152 None:intergenic 55.0%
ATTCCGGTGGAGGTGAGTAC+AGG + chr1.4:418713-418732 None:intergenic 55.0%
CAAGGGAGGAGTAGGTGGTT+TGG + chr1.4:418598-418617 None:intergenic 55.0%
CCCCGATACTCCTCTTAAGC+TGG - chr1.4:418985-419004 MS.gene88984:intron 55.0%
CTAGTAACCGCGGATCAACC+TGG - chr1.4:418647-418666 MS.gene88984:intron 55.0%
CTTCGCTACAGCAACTCTGC+AGG - chr1.4:418734-418753 MS.gene88984:intron 55.0%
GCTAGCAGATCTGGGTGGAA+TGG + chr1.4:417529-417548 None:intergenic 55.0%
GCTGTAGCGAAGAATTCCGG+TGG + chr1.4:418726-418745 None:intergenic 55.0%
GGATTCCACAAAGCTGCTGG+TGG - chr1.4:418063-418082 MS.gene88984:CDS 55.0%
GGTGGAGGTGAGTACAGGAT+TGG + chr1.4:418708-418727 None:intergenic 55.0%
GTAGCGAAGAATTCCGGTGG+AGG + chr1.4:418723-418742 None:intergenic 55.0%
GTGAACGTCAAGGGAGGAGT+AGG + chr1.4:418606-418625 None:intergenic 55.0%
GTGAAGCTAGCAGATCTGGG+TGG + chr1.4:417534-417553 None:intergenic 55.0%
TCACCAGCGGGATCATCGAA+GGG + chr1.4:418132-418151 None:intergenic 55.0%
TCCTCCCTTGACGTTCACGT+GGG - chr1.4:418608-418627 MS.gene88984:intron 55.0%
TGAACCAAGCCCGCTCCATT+AGG - chr1.4:418810-418829 MS.gene88984:intron 55.0%
! AATGGAGCGGGCTTGGTTCA+AGG + chr1.4:418810-418829 None:intergenic 55.0%
! GATCCTTGCGGGTTTGGTCT+TGG + chr1.4:418857-418876 None:intergenic 55.0%
! GGAGGAGTAGGTGGTTTGGA+GGG + chr1.4:418594-418613 None:intergenic 55.0%
! TTGTCCTAATGGAGCGGGCT+TGG + chr1.4:418817-418836 None:intergenic 55.0%
!! ATTTGGATGGTGGGTCCACC+AGG + chr1.4:418779-418798 None:intergenic 55.0%
!! GTCTTGACCAGGTTGATCCG+CGG + chr1.4:418657-418676 None:intergenic 55.0%
!! GTCTTGGTCCACTTGCTGTC+AGG + chr1.4:418841-418860 None:intergenic 55.0%
!! TCCACTTGGTGTTCGCCAAC+AGG - chr1.4:416931-416950 MS.gene88984:CDS 55.0%
!! TTTGGATGGTGGGTCCACCA+GGG + chr1.4:418778-418797 None:intergenic 55.0%
ACCCACGTGAACGTCAAGGG+AGG + chr1.4:418612-418631 None:intergenic 60.0%
CTCCTCCCTTGACGTTCACG+TGG - chr1.4:418607-418626 MS.gene88984:intron 60.0%
GCGGGATCATCGAAGGGAAC+TGG + chr1.4:418126-418145 None:intergenic 60.0%
GGACCAAGACCAAACCCGCA+AGG - chr1.4:418851-418870 MS.gene88984:intron 60.0%
GTTCCCTTCGATGATCCCGC+TGG - chr1.4:418126-418145 MS.gene88984:intron 60.0%
! GGGAGGAGTAGGTGGTTTGG+AGG + chr1.4:418595-418614 None:intergenic 60.0%
!! TTGGATGGTGGGTCCACCAG+GGG + chr1.4:418777-418796 None:intergenic 60.0%
!!! GACTTTTGAGGCAGGCAGGC+AGG + chr1.4:417680-417699 None:intergenic 60.0%
CCAGGGGGTGGACCTGAAAC+TGG + chr1.4:418761-418780 None:intergenic 65.0%
! CCAGTTTCAGGTCCACCCCC+TGG - chr1.4:418758-418777 MS.gene88984:intron 65.0%
! GTTTCAGGTCCACCCCCTGG+TGG - chr1.4:418761-418780 MS.gene88984:intron 65.0%
! TTTGAGGCAGGCAGGCAGGC+AGG + chr1.4:417676-417695 None:intergenic 65.0%
!! TGGATGGTGGGTCCACCAGG+GGG + chr1.4:418776-418795 None:intergenic 65.0%
!! ATGGTGGGTCCACCAGGGGG+TGG + chr1.4:418773-418792 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 416812 419568 416812 ID=MS.gene88984
chr1.4 mRNA 416812 419568 416812 ID=MS.gene88984.t1;Parent=MS.gene88984
chr1.4 exon 419428 419568 419428 ID=MS.gene88984.t1.exon1;Parent=MS.gene88984.t1
chr1.4 CDS 419428 419568 419428 ID=cds.MS.gene88984.t1;Parent=MS.gene88984.t1
chr1.4 exon 419196 419305 419196 ID=MS.gene88984.t1.exon2;Parent=MS.gene88984.t1
chr1.4 CDS 419196 419305 419196 ID=cds.MS.gene88984.t1;Parent=MS.gene88984.t1
chr1.4 exon 418186 418456 418186 ID=MS.gene88984.t1.exon3;Parent=MS.gene88984.t1
chr1.4 CDS 418186 418456 418186 ID=cds.MS.gene88984.t1;Parent=MS.gene88984.t1
chr1.4 exon 418010 418112 418010 ID=MS.gene88984.t1.exon4;Parent=MS.gene88984.t1
chr1.4 CDS 418010 418112 418010 ID=cds.MS.gene88984.t1;Parent=MS.gene88984.t1
chr1.4 exon 417183 417894 417183 ID=MS.gene88984.t1.exon5;Parent=MS.gene88984.t1
chr1.4 CDS 417183 417894 417183 ID=cds.MS.gene88984.t1;Parent=MS.gene88984.t1
chr1.4 exon 416812 417103 416812 ID=MS.gene88984.t1.exon6;Parent=MS.gene88984.t1
chr1.4 CDS 416812 417103 416812 ID=cds.MS.gene88984.t1;Parent=MS.gene88984.t1
Gene Sequence

>MS.gene88984

ATGGCACTGAACTTGGTGTTATTATGTGTTAGTGTTTACCTCTTTGCCATCACTACTACTATTGTTGCTGAAGATCCATACAGGTTCTTCAACTGGAACGTTACCTATGGTGACATTTATCCACTTGGTGTTCGCCAACAGGTATATTTATATTTCACTTTCATCCTTATACCTTATTGGATCTGAATCATTTTGATTAATTAATTGATTCATCAACTAGATAGACCAAAGTTAATTTGTTATGTTTTGTGACAAACTTGCAGGGAATACTTATCAATGGCCAATTCCCAGGACCAGACATTCATTCTGTGACAAATGATAATCTCATTATCAATGTCTTCAACAGCTTGGACGAACCTTTTCTCATTTCATGGTTAGTTAGTACCTTTCACATTTCTTTTTTCATCCATCCTAGCCTCTATCTATGTGTAAATAACAAAAAGAATTAAACACGAAAGCATGTTATGTTGTTAGAGTTGGTTTCTTTTAATGATGTTCCTGTTAGTTAGTTAGCTAGCCACACGTTTGATATCTTCATATTCATCATATCATACTACCTAGTACTACTACATTTTGGGATGTTTGTTTGTTGGGTCACACCACTTGTATTGTGTTGTGTGGCTGCAAAGGATAGGAAAATGATGAAACCTTACAAAACACACATACTATACTATCTTATCTCTTGTAGTACTATGCTGGTCATGGTGGGTCAATCCATTCCACCCAGATCTGCTAGCTTCACTTTTATATTCATTTATATGGAGATGGAAGACATTTTCACAAGTAATATTAATATTCAATTCCAAATTAAGTAATTGGCCAGCACTGCAATTTGGCTTTCTATACTTGCTTGCTTGCTTGCCTGCCTGCCTGCCTGCCTCAAAAGTCTTTTTAATTGGGTGGACCCCATTTACTTTATTCATGCTTTGCTGTTCTCCTCTAGCTAACCATCAAATTGAGCTAATTATCTCCTTGATTACGTGAAATAGTAGTATATTAGGGACCACTACTTTTTAAATTATTGTGAATTTGTGATAATACTAACTGATAAGACATATAACCTTGCAATTGATTAATAAATGATGACATGACATGACATGACATAAAAACAGGAATGGAGTCCAGCAAAGAAGGAACTCATACGAAGATGGAGTGTTTGGAACAACATGTCCCATTCCACCTGGAAAGAACTTTACATACATACTTCAAGTGAAAGATCAAATTGGTAGTTTCTATTATTTCCCATCTCTTGGATTCCACAAAGCTGCTGGTGGTTTTGGAGGAATTAGAATCCTAAGTACGCCAAGAATTCCAGTTCCCTTCGATGATCCCGCTGGTGATTACACTGTCCTTATTGGTGATTGGTACAAGTCCAACCACACGGTAACTAATCATTTACTATCTTCATCATCAATTTCATTTTCAATAAAATTGATTGTATTGTATTATGTGACAGGATTTGAAGGCCCAACTTGATAACGGAAACAAGTTACCTTTCCCTGATGGAATTCTTATCAATGGTCGTGGGTCTGGAGGATCATCTCTCAATGTGGAGCAAGGTACTGCATTACCTACATGAATAAGACTCATATAATGTTTCATTTGGCTTTCCTCAACATTAGTACTAAATTTGACACAACTAATATTTTTTGGCCCTTGTCATTGATGTTACAGGAAAGACTTACAGGCTTAGAATATCAAATGTGGGATCGGAAAATTCACTCAACTTTCGCATACAGAATCACAAGATGAAGCTGGTAGAAGTGGAAGGAACACACACCCTCCAAACCACCTACTCCTCCCTTGACGTTCACGTGGGTCAATCATACTCAGTTCTAGTAACCGCGGATCAACCTGGTCAAGACTATTATATTGTTGTTTCCTCTCGATTTACCAATCCTGTACTCACCTCCACCGGAATTCTTCGCTACAGCAACTCTGCAGGTCCAGTTTCAGGTCCACCCCCTGGTGGACCCACCATCCAAATTGATTGGTCCTTGAACCAAGCCCGCTCCATTAGGACAAACCTGACAGCAAGTGGACCAAGACCAAACCCGCAAGGATCATACCATTATGGCATGATAAACTTAACGAGGACAATTGTGCTATCAAGTTCAGCTGGACAAGTTAATGGGAAACAAAGATACGCAATTAACAGTGTGTCTTATGTTGCCCCCGATACTCCTCTTAAGCTGGCTGACTTCTTCAAGATAGCAGGTGTTTACCGTCCTGGAAGCATCTCTGATAGACCTAGTGGTGGTGGTATTTATCTCGACACTTCTGTTATGCAAACTGACTATAGAACATTTGTTGAGATTGTTTTCCAAAATGATGAAGACATCCTTCAGAGCTATCATCTTGATGGCTACTCTTTCTTTGTTGTTGGGTGAGTATGATTTTCCATCATATAGATCATTTTCATTTCTTTAAAATCAGTCTAATCTATATATGAATGTTGCATGCAGTATGGATGGAGGACAATGGACAAGTGATAAAAGGAACCAGTACAATCTTAGAGACGCAGTTTCGCGTTCCACCACTCAGGTATATCCAAAGTCATGGACTGCTATCTATGTTGCACTTGACAATGTAGGAATGTGGAACTTGAGGTCAGAATTTTGGGCACGACAATATCTCGGGCAACAGTTTTATATGCGTGTATACACAACATCAACATCACTCAGAGATGAATACCCTATTCCTAAGAATGCATTACTTTGTGGTAGGGCAACTGGAAGACATACTCGACCCCTTTGA
Protein sequence

>MS.gene88984.t1

MALNLVLLCVSVYLFAITTTIVAEDPYRFFNWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLIINVFNSLDEPFLISWNGVQQRRNSYEDGVFGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLGFHKAAGGFGGIRILSTPRIPVPFDDPAGDYTVLIGDWYKSNHTDLKAQLDNGNKLPFPDGILINGRGSGGSSLNVEQGKTYRLRISNVGSENSLNFRIQNHKMKLVEVEGTHTLQTTYSSLDVHVGQSYSVLVTADQPGQDYYIVVSSRFTNPVLTSTGILRYSNSAGPVSGPPPGGPTIQIDWSLNQARSIRTNLTASGPRPNPQGSYHYGMINLTRTIVLSSSAGQVNGKQRYAINSVSYVAPDTPLKLADFFKIAGVYRPGSISDRPSGGGIYLDTSVMQTDYRTFVEIVFQNDEDILQSYHLDGYSFFVVGMDGGQWTSDKRNQYNLRDAVSRSTTQVYPKSWTAIYVALDNVGMWNLRSEFWARQYLGQQFYMRVYTTSTSLRDEYPIPKNALLCGRATGRHTRPL