AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89031

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89031.t1 MTR_2g103650 97.704 392 9 0 1 392 13 404 0.0 786
MS.gene89031.t1 MTR_5g043350 46.702 379 191 4 14 390 27 396 1.80e-116 345
MS.gene89031.t1 MTR_3g054320 46.950 377 184 5 16 390 30 392 1.86e-113 337
MS.gene89031.t1 MTR_3g084380 46.316 380 190 5 14 390 19 387 3.14e-111 331
MS.gene89031.t1 MTR_6g028080 44.094 381 196 6 15 390 29 397 3.31e-110 329
MS.gene89031.t1 MTR_4g084050 44.125 383 195 5 16 390 23 394 9.50e-109 325
MS.gene89031.t1 MTR_3g084360 44.180 378 199 4 15 390 23 390 1.21e-108 324
MS.gene89031.t1 MTR_8g010080 45.623 377 182 8 16 390 31 386 9.17e-105 314
MS.gene89031.t1 MTR_0157s0060 46.011 376 183 5 19 390 1 360 2.12e-103 310
MS.gene89031.t1 MTR_4g034020 46.011 376 183 5 19 390 1 360 2.12e-103 310
MS.gene89031.t1 MTR_8g010070 43.799 379 192 7 16 392 31 390 1.37e-101 306
MS.gene89031.t1 MTR_8g010040 43.799 379 192 8 2 379 18 376 2.21e-96 293
MS.gene89031.t1 MTR_1g012390 41.146 384 213 7 12 392 24 397 4.81e-95 290
MS.gene89031.t1 MTR_2g103410 41.803 366 207 5 16 380 59 419 5.66e-94 289
MS.gene89031.t1 MTR_1g052315 41.834 349 196 4 11 358 59 401 2.62e-92 285
MS.gene89031.t1 MTR_8g010110 42.175 377 196 7 16 390 31 387 2.65e-91 280
MS.gene89031.t1 MTR_8g008765 45.015 331 161 8 16 344 31 342 4.32e-91 278
MS.gene89031.t1 MTR_8g010090 41.005 378 199 9 16 390 31 387 3.57e-88 272
MS.gene89031.t1 MTR_8g065030 39.669 363 211 5 17 378 66 421 1.11e-87 273
MS.gene89031.t1 MTR_6g005630 38.281 384 221 10 11 392 40 409 2.30e-85 266
MS.gene89031.t1 MTR_7g091850 40.608 362 207 6 1 360 61 416 4.02e-84 263
MS.gene89031.t1 MTR_4g094715 36.340 388 235 7 11 390 60 443 2.11e-83 262
MS.gene89031.t1 MTR_8g010050 39.257 377 174 7 16 390 31 354 8.41e-83 257
MS.gene89031.t1 MTR_1g013740 36.729 373 212 6 10 381 29 378 1.91e-82 257
MS.gene89031.t1 MTR_7g091840 42.769 325 179 6 9 330 57 377 1.95e-81 254
MS.gene89031.t1 MTR_2g060350 39.942 343 198 5 18 358 76 412 4.33e-80 253
MS.gene89031.t1 MTR_1g086390 36.104 385 236 7 16 392 81 463 9.44e-79 250
MS.gene89031.t1 MTR_8g077320 37.984 387 171 8 16 389 33 363 1.95e-78 246
MS.gene89031.t1 MTR_1g013730 35.389 373 217 6 10 381 21 370 8.41e-78 245
MS.gene89031.t1 MTR_7g035415 42.357 314 166 5 7 318 31 331 1.00e-77 243
MS.gene89031.t1 MTR_8g065010 35.539 408 228 10 12 390 103 504 1.16e-77 249
MS.gene89031.t1 MTR_5g034090 34.975 406 230 10 13 390 118 517 1.89e-77 249
MS.gene89031.t1 MTR_3g438050 37.297 370 216 8 13 377 24 382 5.16e-77 243
MS.gene89031.t1 MTR_7g110050 33.938 386 242 7 16 392 67 448 3.29e-76 243
MS.gene89031.t1 MTR_5g033680 34.771 371 232 6 15 380 58 423 8.48e-76 243
MS.gene89031.t1 MTR_8g008700 41.640 317 145 7 78 392 14 292 6.39e-75 234
MS.gene89031.t1 MTR_3g030540 40.741 351 200 5 11 360 65 408 1.90e-74 238
MS.gene89031.t1 MTR_6g005630 35.938 384 207 12 11 392 40 386 4.64e-72 231
MS.gene89031.t1 MTR_2g032710 34.884 387 240 7 12 390 63 445 2.02e-70 228
MS.gene89031.t1 MTR_4g120320 38.547 358 206 10 10 361 46 395 1.11e-68 223
MS.gene89031.t1 MTR_4g083660 37.565 386 226 9 8 390 1 374 1.20e-67 219
MS.gene89031.t1 MTR_1g088160 36.415 357 216 7 16 369 35 383 2.22e-67 219
MS.gene89031.t1 MTR_2g049400 36.458 384 223 12 1 378 11 379 2.54e-66 216
MS.gene89031.t1 MTR_8g030930 34.115 384 232 9 16 390 27 398 9.95e-65 212
MS.gene89031.t1 MTR_2g103410 42.586 263 146 4 16 277 59 317 5.19e-64 208
MS.gene89031.t1 MTR_7g113910 37.500 304 154 5 79 381 74 342 1.72e-61 202
MS.gene89031.t1 MTR_8g009810 34.417 369 223 9 15 378 34 388 3.11e-60 200
MS.gene89031.t1 MTR_8g031180 41.751 297 135 8 79 368 15 280 4.06e-60 196
MS.gene89031.t1 MTR_2g058670 35.843 332 204 6 34 361 41 367 5.09e-60 200
MS.gene89031.t1 MTR_2g058640 36.119 335 199 8 34 361 41 367 6.43e-60 199
MS.gene89031.t1 MTR_2g435640 36.257 342 207 8 16 353 53 387 7.62e-60 200
MS.gene89031.t1 MTR_5g063430 38.284 303 167 9 16 313 25 312 2.29e-58 192
MS.gene89031.t1 MTR_2g058840 34.604 341 194 8 34 361 41 365 2.98e-58 195
MS.gene89031.t1 MTR_5g034090 36.426 291 155 6 13 277 118 404 4.70e-57 192
MS.gene89031.t1 MTR_2g029170 36.286 350 197 12 50 390 29 361 7.56e-57 190
MS.gene89031.t1 MTR_5g097860 33.724 341 207 10 1 334 10 338 2.51e-53 181
MS.gene89031.t1 MTR_2g100040 29.396 381 200 13 1 374 10 328 1.43e-38 142
MS.gene89031.t1 MTR_7g088550 28.155 309 170 11 20 293 54 345 2.23e-22 99.0
MS.gene89031.t1 MTR_7g110710 24.471 425 252 16 15 384 42 452 3.48e-22 98.2
MS.gene89031.t1 MTR_7g110710 24.471 425 252 16 15 384 42 452 3.89e-22 98.2
MS.gene89031.t1 MTR_1g100040 24.495 396 256 11 20 385 60 442 4.74e-22 97.8
MS.gene89031.t1 MTR_1g100050 24.495 396 256 11 20 385 54 436 4.91e-21 95.1
MS.gene89031.t1 MTR_1g099850 25.126 398 251 11 20 385 59 441 5.60e-21 94.7
MS.gene89031.t1 MTR_4g124980 24.877 406 240 14 16 380 74 455 6.70e-21 94.7
MS.gene89031.t1 MTR_1g100060 26.962 293 180 7 20 286 97 381 9.17e-21 94.4
MS.gene89031.t1 MTR_8g098840 24.533 375 222 13 17 342 45 407 2.12e-20 93.2
MS.gene89031.t1 MTR_2g042740 24.879 414 230 14 16 380 56 437 6.28e-20 91.7
MS.gene89031.t1 MTR_8g028725 24.495 396 256 10 20 385 59 441 4.36e-19 89.4
MS.gene89031.t1 MTR_7g078680 27.000 300 177 9 17 286 56 343 1.54e-17 84.3
MS.gene89031.t1 MTR_3g449590 26.480 321 187 9 15 302 46 350 2.27e-17 84.0
MS.gene89031.t1 MTR_5g090160 25.382 327 188 12 13 302 41 348 4.15e-17 83.2
MS.gene89031.t1 MTR_3g096330 26.183 317 184 12 18 300 78 378 1.07e-16 82.4
MS.gene89031.t1 MTR_1g086030 24.299 321 202 9 15 306 81 389 1.49e-16 81.6
MS.gene89031.t1 MTR_1g086030 28.241 216 135 5 103 306 78 285 6.21e-16 79.3
MS.gene89031.t1 MTR_4g120730 24.234 359 216 12 15 336 39 378 1.18e-13 72.4
MS.gene89031.t1 MTR_7g072360 31.967 122 61 1 154 275 29 128 6.71e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89031.t1 AT5G17200 45.074 406 185 7 7 390 30 419 2.05e-112 335
MS.gene89031.t1 AT5G27530 42.494 393 202 3 1 390 38 409 1.77e-106 321
MS.gene89031.t1 AT4G35670 42.494 393 210 4 1 390 9 388 2.31e-102 308
MS.gene89031.t1 AT5G44840 39.744 390 226 4 1 390 9 389 3.73e-100 303
MS.gene89031.t1 AT3G15720 39.695 393 219 7 3 390 9 388 1.48e-95 293
MS.gene89031.t1 AT3G15720 39.695 393 219 7 3 390 10 389 1.85e-95 293
MS.gene89031.t1 AT3G15720 39.695 393 216 8 3 390 9 385 5.78e-94 289
MS.gene89031.t1 AT4G32380 39.842 379 206 4 1 377 24 382 1.83e-93 288
MS.gene89031.t1 AT1G80170 39.892 371 218 4 12 381 49 415 3.08e-92 284
MS.gene89031.t1 AT1G56710 39.433 388 222 6 14 392 46 429 4.27e-89 276
MS.gene89031.t1 AT3G57510 41.620 358 199 7 14 368 66 416 5.02e-89 276
MS.gene89031.t1 AT5G44830 40.401 349 189 3 42 390 1 330 5.61e-89 272
MS.gene89031.t1 AT1G70500 37.995 379 223 5 3 378 54 423 7.85e-86 268
MS.gene89031.t1 AT2G41850 41.598 363 202 7 2 361 54 409 2.61e-85 266
MS.gene89031.t1 AT1G23460 38.567 363 215 5 17 378 44 399 2.72e-84 263
MS.gene89031.t1 AT1G23460 38.567 363 215 5 17 378 68 423 6.39e-84 263
MS.gene89031.t1 AT5G14650 38.320 381 224 8 12 390 47 418 1.13e-81 257
MS.gene89031.t1 AT1G48100 36.504 389 235 7 12 392 76 460 1.24e-81 258
MS.gene89031.t1 AT1G02460 36.693 387 232 8 16 391 89 473 4.31e-80 254
MS.gene89031.t1 AT3G07970 39.255 349 205 5 11 358 66 408 1.06e-79 252
MS.gene89031.t1 AT4G01890 36.387 393 235 9 10 390 59 448 1.68e-79 252
MS.gene89031.t1 AT1G10640 35.714 406 227 11 13 390 116 515 1.37e-78 252
MS.gene89031.t1 AT1G60590 35.194 412 230 10 10 390 117 522 4.95e-78 250
MS.gene89031.t1 AT4G32380 35.809 377 183 4 1 377 24 341 1.10e-77 246
MS.gene89031.t1 AT3G15720 38.393 336 191 5 59 390 2 325 5.58e-77 243
MS.gene89031.t1 AT1G05660 36.387 393 232 10 2 390 14 392 2.14e-75 239
MS.gene89031.t1 AT1G05650 37.435 382 221 10 2 379 14 381 4.16e-75 238
MS.gene89031.t1 AT3G26610 35.484 372 227 6 16 379 67 433 1.34e-74 239
MS.gene89031.t1 AT4G35670 43.345 293 150 4 101 390 2 281 7.55e-72 227
MS.gene89031.t1 AT2G43880 37.368 380 219 9 15 390 30 394 2.46e-71 229
MS.gene89031.t1 AT4G32370 41.549 284 153 4 32 310 51 326 6.80e-71 226
MS.gene89031.t1 AT2G43890 37.288 354 207 9 1 350 12 354 1.82e-70 226
MS.gene89031.t1 AT1G02790 37.719 342 195 9 14 353 50 375 8.41e-69 223
MS.gene89031.t1 AT1G78400 36.800 375 219 8 10 378 29 391 4.71e-68 221
MS.gene89031.t1 AT2G43860 38.021 384 219 12 14 392 31 400 2.73e-67 219
MS.gene89031.t1 AT1G80140 36.471 340 204 4 54 390 6 336 3.17e-67 216
MS.gene89031.t1 AT1G80170 42.697 267 148 4 12 277 49 311 1.48e-66 214
MS.gene89031.t1 AT1G43080 34.243 403 242 11 1 390 11 403 3.27e-66 216
MS.gene89031.t1 AT4G32375 39.860 286 163 2 92 376 4 281 4.01e-66 216
MS.gene89031.t1 AT2G15460 35.829 374 220 11 16 380 31 393 1.25e-65 214
MS.gene89031.t1 AT1G43090 35.310 371 222 10 16 378 31 391 3.66e-65 214
MS.gene89031.t1 AT2G15450 35.561 374 221 11 16 380 31 393 5.23e-65 213
MS.gene89031.t1 AT2G26620 34.771 371 226 9 16 380 31 391 8.28e-65 212
MS.gene89031.t1 AT2G40310 33.419 389 231 10 15 390 30 403 1.20e-64 212
MS.gene89031.t1 AT4G13760 36.127 346 202 10 16 353 31 365 1.76e-64 211
MS.gene89031.t1 AT1G65570 35.770 383 229 11 1 379 17 386 1.88e-64 211
MS.gene89031.t1 AT5G39910 34.066 364 179 5 2 363 23 327 3.24e-64 210
MS.gene89031.t1 AT2G43870 34.343 396 237 14 1 390 6 384 7.41e-64 209
MS.gene89031.t1 AT1G43100 34.271 391 234 11 1 378 11 391 1.26e-63 210
MS.gene89031.t1 AT4G18180 35.028 354 220 6 11 360 34 381 1.53e-63 209
MS.gene89031.t1 AT2G15470 35.294 374 222 11 16 380 31 393 1.69e-63 209
MS.gene89031.t1 AT3G59850 37.069 348 204 9 15 358 22 358 4.12e-63 207
MS.gene89031.t1 AT1G17150 34.474 380 233 10 16 390 33 401 4.45e-63 207
MS.gene89031.t1 AT3G59850 37.249 349 202 10 15 358 66 402 9.27e-63 207
MS.gene89031.t1 AT2G33160 34.946 372 221 10 16 378 32 391 9.05e-62 210
MS.gene89031.t1 AT3G07820 35.347 331 201 7 34 361 38 358 1.02e-58 196
MS.gene89031.t1 AT5G14650 40.824 267 149 6 12 277 47 305 1.32e-58 193
MS.gene89031.t1 AT5G48140 35.347 331 200 7 34 361 39 358 5.97e-58 194
MS.gene89031.t1 AT2G43880 38.333 300 174 7 15 313 30 319 5.98e-57 189
MS.gene89031.t1 AT3G07830 32.609 368 221 9 34 387 39 393 6.43e-55 186
MS.gene89031.t1 AT3G14040 35.356 379 219 10 15 387 70 428 6.41e-53 182
MS.gene89031.t1 AT3G07850 34.961 389 227 10 9 391 63 431 6.47e-53 182
MS.gene89031.t1 AT3G07840 34.743 331 203 8 34 361 39 359 1.03e-52 181
MS.gene89031.t1 AT2G23900 25.310 403 245 15 20 385 60 443 1.48e-24 105
MS.gene89031.t1 AT3G62110 24.938 401 266 10 15 389 42 433 1.01e-23 102
MS.gene89031.t1 AT3G62110 24.938 401 266 10 15 389 42 433 1.01e-23 102
MS.gene89031.t1 AT3G42950 26.471 408 231 13 16 380 66 447 5.62e-23 100
MS.gene89031.t1 AT3G48950 26.367 311 181 11 20 294 49 347 3.64e-22 98.2
MS.gene89031.t1 AT3G61490 28.000 300 174 9 17 286 159 446 4.71e-22 98.6
MS.gene89031.t1 AT3G61490 28.000 300 174 9 17 286 61 348 6.23e-22 97.8
MS.gene89031.t1 AT3G61490 28.000 300 174 9 17 286 46 333 8.41e-22 97.4
MS.gene89031.t1 AT3G61490 28.000 300 174 9 17 286 46 333 8.41e-22 97.4
MS.gene89031.t1 AT3G61490 28.000 300 174 9 17 286 46 333 8.41e-22 97.4
MS.gene89031.t1 AT4G33440 28.438 320 177 12 16 300 72 374 1.40e-20 93.6
MS.gene89031.t1 AT4G23500 23.592 373 224 12 20 343 72 432 7.01e-18 85.5
MS.gene89031.t1 AT5G41870 26.266 316 193 8 16 302 45 349 2.92e-17 83.6
MS.gene89031.t1 AT4G23820 26.498 317 188 10 17 302 42 344 6.19e-17 82.4
MS.gene89031.t1 AT1G19170 23.940 401 251 15 16 380 87 469 1.09e-15 79.0
MS.gene89031.t1 AT3G16850 22.881 354 203 10 15 317 39 373 4.94e-12 67.8
MS.gene89031.t1 AT5G49215 23.642 313 185 11 18 294 45 339 8.94e-11 63.5
MS.gene89031.t1 AT5G49215 23.642 313 185 11 18 294 43 337 9.14e-11 63.5

Find 87 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCAATCCATGTGTTTATT+TGG 0.080796 2.4:-1942715 None:intergenic
ATATCAATGCCATCAGTATT+AGG 0.225865 2.4:-1943260 None:intergenic
TGCTATCGATTTCAAATAAA+AGG 0.252766 2.4:+1943140 MS.gene89031:CDS
TTTGATTAACACAATAAAAT+TGG 0.261059 2.4:-1944032 None:intergenic
AACTCTAACAAGCTGCAAAA+AGG 0.263487 2.4:+1943196 MS.gene89031:CDS
ACCTCAATGTATATATAATT+AGG 0.293049 2.4:-1941256 None:intergenic
ACCAATCCATGTGTTTATTT+GGG 0.310797 2.4:-1942714 None:intergenic
TAAAATTGGTCAATCATAAT+GGG 0.312360 2.4:-1944018 None:intergenic
GGTTGGAGCATCCCACATTT+TGG 0.315541 2.4:-1944272 None:intergenic
GGTCAATCATAATGGGGTTA+TGG 0.323679 2.4:-1944011 None:intergenic
ACTTTCAATGTATTGCAATA+TGG 0.324810 2.4:+1940504 MS.gene89031:CDS
TGCAGCTTGTTAGAGTTATA+TGG 0.325682 2.4:-1943190 None:intergenic
ATAAAATTGGTCAATCATAA+TGG 0.333850 2.4:-1944019 None:intergenic
AAGGAGAAAACCTTCATCTT+AGG 0.335531 2.4:-1944330 None:intergenic
AAAACAAAATCGGAATATTC+AGG 0.348846 2.4:+1942689 MS.gene89031:CDS
ACGTTGACTGAAACATTAAC+TGG 0.363004 2.4:+1943717 MS.gene89031:CDS
CATGGACCACGGAAGGCTAT+TGG 0.371597 2.4:-1941229 None:intergenic
CTTAACTCTTTCATTGCAAC+AGG 0.372607 2.4:+1943308 MS.gene89031:CDS
TTGAGCAAGCAGTTTAGAAT+TGG 0.374243 2.4:-1944390 None:intergenic
GTCAATCATAATGGGGTTAT+GGG 0.383263 2.4:-1944010 None:intergenic
AGGCTCTTCCATGAGCATTA+TGG 0.387155 2.4:-1944356 None:intergenic
TTAATTTACAGGGGAATCAT+TGG 0.387222 2.4:-1940547 None:intergenic
TATCAATGCCATCAGTATTA+GGG 0.410347 2.4:-1943259 None:intergenic
CAAGCAGTTTAGAATTGGTT+TGG 0.412319 2.4:-1944385 None:intergenic
GTGAATGGTTTAATTATAAG+TGG 0.419645 2.4:+1942746 MS.gene89031:CDS
AGTGGAAAGGGGACTGTTGA+TGG 0.421230 2.4:+1942764 MS.gene89031:CDS
AGAGTTATATGGCTTCTTGC+TGG 0.423641 2.4:-1943179 None:intergenic
GTGGTCCAGGACATGGTATC+AGG 0.443137 2.4:+1943496 MS.gene89031:CDS
ATGCACATCCTCTACAATAT+CGG 0.446275 2.4:-1943682 None:intergenic
AGAAATTTACTTACGGGTGG+TGG 0.450674 2.4:-1942827 None:intergenic
AACATCCGTCTAATTGCTCC+TGG 0.450766 2.4:+1943230 MS.gene89031:CDS
TGTTGCACCTAAAACAAAAT+CGG 0.452223 2.4:+1942679 MS.gene89031:CDS
GGTTTAATTATAAGTGGAAA+GGG 0.462142 2.4:+1942752 MS.gene89031:CDS
GTGAGTGATGTAACATATAG+AGG 0.462577 2.4:+1944201 MS.gene89031:CDS
GATGTGTGTCAAAGCAAATC+AGG 0.463712 2.4:+1941160 MS.gene89031:CDS
TCCTTCTGCCATAATGCTCA+TGG 0.470413 2.4:+1944348 MS.gene89031:CDS
GATTGCATTGCTATTAGTGC+TGG 0.472202 2.4:+1943441 MS.gene89031:CDS
AGTAAGAATGTCTTGGCTGC+TGG 0.474123 2.4:-1941202 None:intergenic
ATTGGTTTCTCATTTGTGAA+TGG 0.475752 2.4:+1942731 MS.gene89031:CDS
ATCTGCTGTTCCTAAGATGA+AGG 0.477417 2.4:+1944320 MS.gene89031:CDS
ATTTCTGAACAGGGTGGAGG+TGG 0.479630 2.4:+1943943 MS.gene89031:intron
TCCCAAATAAACACATGGAT+TGG 0.484532 2.4:+1942713 MS.gene89031:CDS
TATATATAATTAGGTTTGCA+TGG 0.485064 2.4:-1941247 None:intergenic
GTAACATATAGAGGGATTAG+TGG 0.503191 2.4:+1944210 MS.gene89031:CDS
GTATTGCAATATGGAGCAGT+TGG 0.517751 2.4:+1940513 MS.gene89031:CDS
CAATATGGAGCAGTTGGAGA+TGG 0.520589 2.4:+1940519 MS.gene89031:CDS
ACCAACCTGATACCATGTCC+TGG 0.526714 2.4:-1943501 None:intergenic
TGTATTTCTGAACAGGGTGG+AGG 0.533598 2.4:+1943940 MS.gene89031:intron
TTTGAACTGCGACCAAAATG+TGG 0.534380 2.4:+1944260 MS.gene89031:CDS
TGGCCTCAGTAAGAATGTCT+TGG 0.534716 2.4:-1941209 None:intergenic
TGGTTTAATTATAAGTGGAA+AGG 0.535186 2.4:+1942751 MS.gene89031:CDS
AAGGGGACTGTTGATGGTCG+AGG 0.551369 2.4:+1942770 MS.gene89031:CDS
AGGTTTGCATGGACCACGGA+AGG 0.551870 2.4:-1941236 None:intergenic
AACACGATCAAATACAAGGT+TGG 0.555210 2.4:-1944289 None:intergenic
AGTAAGAATCAAGACAAAGC+AGG 0.556906 2.4:+1943740 MS.gene89031:CDS
TCAGTATTAGGGCTCTCTCC+AGG 0.559038 2.4:-1943248 None:intergenic
CATAAACACGATCAAATACA+AGG 0.567940 2.4:-1944293 None:intergenic
TAGGTACTACTTACTGTTGG+TGG 0.572062 2.4:-1942978 None:intergenic
ATTCTAAGTATGTTTAAACT+CGG 0.572318 2.4:+1940465 MS.gene89031:CDS
ACTGCTATAACATGTGGTCC+AGG 0.572929 2.4:+1943483 MS.gene89031:CDS
TAGAATTGGTTTGGTGTGTG+AGG 0.574986 2.4:-1944376 None:intergenic
AGGGTGGAGGTGGATTTGCT+AGG 0.585416 2.4:+1943953 MS.gene89031:intron
GTTTAATTATAAGTGGAAAG+GGG 0.586309 2.4:+1942753 MS.gene89031:CDS
GGTGGTGGATTTCCTATGCA+CGG 0.586917 2.4:-1942812 None:intergenic
GTGGAGGTGGATTTGCTAGG+AGG 0.587173 2.4:+1943956 MS.gene89031:CDS
TGGTGGAAACAACCGTGCAT+AGG 0.588564 2.4:+1942800 MS.gene89031:CDS
GGAGAGAGCCCTAATACTGA+TGG 0.597997 2.4:+1943251 MS.gene89031:CDS
TCTCTCCAGGAGCAATTAGA+CGG 0.599323 2.4:-1943235 None:intergenic
TCCATGAGCATTATGGCAGA+AGG 0.604867 2.4:-1944349 None:intergenic
ATAACATGTGGTCCAGGACA+TGG 0.613113 2.4:+1943489 MS.gene89031:CDS
TGAGTGATGTAACATATAGA+GGG 0.613324 2.4:+1944202 MS.gene89031:CDS
CAGGATCCCAAATAAACACA+TGG 0.616392 2.4:+1942708 MS.gene89031:CDS
ACCTAATTATATATACATTG+AGG 0.616906 2.4:+1941255 MS.gene89031:CDS
AAGTACTTGAATGTCTCTTG+AGG 0.626125 2.4:-1943288 None:intergenic
TACTACTTACTGTTGGTGGA+CGG 0.626963 2.4:-1942974 None:intergenic
AAAATAACTGCTATAACATG+TGG 0.629212 2.4:+1943477 MS.gene89031:CDS
TTGTAGCATTGGATCATTGG+GGG 0.630301 2.4:+1943647 MS.gene89031:intron
CTGAGGCCAATAGCCTTCCG+TGG 0.632375 2.4:+1941223 MS.gene89031:CDS
AAAATTGGTCAATCATAATG+GGG 0.641528 2.4:-1944017 None:intergenic
TTGATGGTCGAGGCTCTATG+TGG 0.642714 2.4:+1942780 MS.gene89031:CDS
AATTAGGTTTGCATGGACCA+CGG 0.648764 2.4:-1941240 None:intergenic
ATGGTCGAGGCTCTATGTGG+TGG 0.666767 2.4:+1942783 MS.gene89031:CDS
TTTGTAGCATTGGATCATTG+GGG 0.669066 2.4:+1943646 MS.gene89031:intron
TGGAGACACCGATATTGTAG+AGG 0.670467 2.4:+1943674 MS.gene89031:CDS
TGGAACATCATTAACAGACA+AGG 0.670604 2.4:+1944230 MS.gene89031:CDS
TTGAACTGCGACCAAAATGT+GGG 0.675802 2.4:+1944261 MS.gene89031:CDS
CAGCCAAGACATTCTTACTG+AGG 0.677474 2.4:+1941206 MS.gene89031:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAATTTAGTTTATTTTTT+GGG - chr2.4:1944122-1944141 None:intergenic 10.0%
!!! TAGAAATTTAGTTTATTTTT+TGG - chr2.4:1944123-1944142 None:intergenic 10.0%
!!! TTAAATTATTTTATCACATT+TGG - chr2.4:1941091-1941110 None:intergenic 10.0%
!! AATAAAGAAATTAATACTCA+TGG - chr2.4:1943811-1943830 None:intergenic 15.0%
!! AGTAACATATATAAGAAAAA+TGG - chr2.4:1944098-1944117 None:intergenic 15.0%
!! ATTGTATCTAATCATATTAT+AGG + chr2.4:1943414-1943433 MS.gene89031:intron 15.0%
!! CAAATGAAATAATTAAAGTT+AGG - chr2.4:1941061-1941080 None:intergenic 15.0%
!! GAAAACTAAAATAAACATAA+GGG + chr2.4:1940896-1940915 MS.gene89031:intron 15.0%
!! GAAAACTAAAATAAACATAA+GGG - chr2.4:1940915-1940896 None:intergenic 15.0%
!! TAGATATAGAAATTTACTTA+CGG - chr2.4:1942837-1942856 None:intergenic 15.0%
!! TGAAAACTAAAATAAACATA+AGG + chr2.4:1940895-1940914 MS.gene89031:intron 15.0%
!! TGAAAACTAAAATAAACATA+AGG - chr2.4:1940914-1940895 None:intergenic 15.0%
!! TTCATATTTGTAAAAGAAAA+TGG + chr2.4:1942077-1942096 MS.gene89031:intron 15.0%
!! TTTGATTAACACAATAAAAT+TGG - chr2.4:1944035-1944054 None:intergenic 15.0%
!!! ATATATTATTTTTGTAGCAT+TGG + chr2.4:1943636-1943655 MS.gene89031:intron 15.0%
!!! ATTTTATTGTGTTAATCAAA+TGG + chr2.4:1944035-1944054 MS.gene89031:CDS 15.0%
!!! ATTTTTTTTTTCTTTTTCAG+GGG + chr2.4:1942943-1942962 MS.gene89031:intron 15.0%
!!! GAAATTTAGTTTATTTTTTG+GGG - chr2.4:1944121-1944140 None:intergenic 15.0%
!!! GATTTTTTTTTTCTTTTTCA+GGG + chr2.4:1942942-1942961 MS.gene89031:intron 15.0%
!!! TGATTTTTTTTTTCTTTTTC+AGG + chr2.4:1942941-1942960 MS.gene89031:intron 15.0%
!!! TGCTATAATATTTTTTCAAT+AGG + chr2.4:1941117-1941136 MS.gene89031:intron 15.0%
!!! TTTCACTTATACTATAATTT+TGG + chr2.4:1943857-1943876 MS.gene89031:intron 15.0%
!!! TTTTATAACCATATTATTTC+TGG + chr2.4:1941458-1941477 MS.gene89031:intron 15.0%
!! AAAATAAATACCAGTTCATA+TGG - chr2.4:1943081-1943100 None:intergenic 20.0%
!! AAAGTTATTAATGTTACACA+TGG - chr2.4:1940619-1940638 None:intergenic 20.0%
!! AATAGTTAAGTACTTTACAA+AGG + chr2.4:1942502-1942521 MS.gene89031:intron 20.0%
!! ACCAAAATAACTAACATTAT+TGG + chr2.4:1943776-1943795 MS.gene89031:intron 20.0%
!! ACCTAATTATATATACATTG+AGG + chr2.4:1941255-1941274 MS.gene89031:CDS 20.0%
!! ACCTCAATGTATATATAATT+AGG - chr2.4:1941259-1941278 None:intergenic 20.0%
!! AGATATAGAAATTTACTTAC+GGG - chr2.4:1942836-1942855 None:intergenic 20.0%
!! ATAAAATTGGTCAATCATAA+TGG - chr2.4:1944022-1944041 None:intergenic 20.0%
!! ATAAGTGAAAAACCTTATAT+AGG - chr2.4:1943847-1943866 None:intergenic 20.0%
!! ATCAATTACCAATGTTATAT+TGG - chr2.4:1941599-1941618 None:intergenic 20.0%
!! ATGAAAAAAATATACTTGAG+AGG - chr2.4:1941518-1941537 None:intergenic 20.0%
!! ATTAATGGAAAGATCAATTA+AGG + chr2.4:1942031-1942050 MS.gene89031:intron 20.0%
!! ATTCTAAGTATGTTTAAACT+CGG + chr2.4:1940465-1940484 MS.gene89031:CDS 20.0%
!! GAAAACTAAAATAAACGTAT+GGG - chr2.4:1940724-1940743 None:intergenic 20.0%
!! GTGTAACATTAATAACTTTA+AGG + chr2.4:1940620-1940639 MS.gene89031:intron 20.0%
!! TAAAATTGGTCAATCATAAT+GGG - chr2.4:1944021-1944040 None:intergenic 20.0%
!! TATATATAATTAGGTTTGCA+TGG - chr2.4:1941250-1941269 None:intergenic 20.0%
!! TGAAAACTAAAATAAACGTA+TGG - chr2.4:1940725-1940744 None:intergenic 20.0%
!! TTAATGGAAAGATCAATTAA+GGG + chr2.4:1942032-1942051 MS.gene89031:intron 20.0%
!! TTAATTGATCTTTCCATTAA+TGG - chr2.4:1942032-1942051 None:intergenic 20.0%
!! TTGAAACTTTCAAAACATAA+GGG - chr2.4:1940695-1940714 None:intergenic 20.0%
!!! ACCAATAATGTTAGTTATTT+TGG - chr2.4:1943780-1943799 None:intergenic 20.0%
!!! ATAAGTGAGTTTTCATTTTT+TGG + chr2.4:1942420-1942439 MS.gene89031:intron 20.0%
!!! ATTGATGTGTTACTTTATTT+AGG + chr2.4:1941613-1941632 MS.gene89031:intron 20.0%
!!! GTTTATTTTAGTTTTCAAGT+CGG - chr2.4:1940687-1940668 None:intergenic 20.0%
!!! GTTTATTTTAGTTTTCAAGT+TGG + chr2.4:1940668-1940687 MS.gene89031:intron 20.0%
!!! GTTTATTTTAGTTTTCAAGT+TGG - chr2.4:1940687-1940668 None:intergenic 20.0%
!!! TATTTATTTTTGTGTGTGTA+TGG + chr2.4:1943091-1943110 MS.gene89031:intron 20.0%
!!! TGAAAACTTTTAAAACGTAA+GGG + chr2.4:1940986-1941005 MS.gene89031:intron 20.0%
!!! TTGAAAACTTTTAAAACGTA+AGG + chr2.4:1940985-1941004 MS.gene89031:intron 20.0%
!!! TTTTGCTATATAATTGGTTA+AGG - chr2.4:1942378-1942397 None:intergenic 20.0%
! AAAACAAAATCGGAATATTC+AGG + chr2.4:1942689-1942708 MS.gene89031:CDS 25.0%
! AAAATAACTGCTATAACATG+TGG + chr2.4:1943477-1943496 MS.gene89031:CDS 25.0%
! AAAATTGGTCAATCATAATG+GGG - chr2.4:1944020-1944039 None:intergenic 25.0%
! AATACTTGCCAGAAATAATA+TGG - chr2.4:1941469-1941488 None:intergenic 25.0%
! ACAAGAGATAAATTCTCAAA+TGG + chr2.4:1942238-1942257 MS.gene89031:intron 25.0%
! ACTTTCAATGTATTGCAATA+TGG + chr2.4:1940504-1940523 MS.gene89031:CDS 25.0%
! AGAGATCATAGTTAATTTAC+AGG - chr2.4:1940561-1940580 None:intergenic 25.0%
! AGATCATAGTTAATTTACAG+GGG - chr2.4:1940559-1940578 None:intergenic 25.0%
! ATGTAAAGTTGCTAGATTAA+TGG - chr2.4:1943603-1943622 None:intergenic 25.0%
! CACTTTAACCAATATAACAT+TGG + chr2.4:1941588-1941607 MS.gene89031:intron 25.0%
! CAGATCATAATCTTTAATGT+AGG + chr2.4:1941758-1941777 MS.gene89031:intron 25.0%
! CTACATTAAAGATTATGATC+TGG - chr2.4:1941760-1941779 None:intergenic 25.0%
! CTTGAAACTTTCAAAACATA+AGG - chr2.4:1940696-1940715 None:intergenic 25.0%
! GAGATCATAGTTAATTTACA+GGG - chr2.4:1940560-1940579 None:intergenic 25.0%
! GGTTTAATTATAAGTGGAAA+GGG + chr2.4:1942752-1942771 MS.gene89031:CDS 25.0%
! GTGAATGGTTTAATTATAAG+TGG + chr2.4:1942746-1942765 MS.gene89031:CDS 25.0%
! GTTTAATTATAAGTGGAAAG+GGG + chr2.4:1942753-1942772 MS.gene89031:CDS 25.0%
! TGATCAATTACCTATAACAT+GGG - chr2.4:1941812-1941831 None:intergenic 25.0%
! TGATGCAAGTATTACTATAA+AGG - chr2.4:1942915-1942934 None:intergenic 25.0%
! TGGTTTAATTATAAGTGGAA+AGG + chr2.4:1942751-1942770 MS.gene89031:CDS 25.0%
! TTGAAACTTTCAAAACGTAA+GGG + chr2.4:1940956-1940975 MS.gene89031:intron 25.0%
! TTGAAACTTTCAAAACGTAA+GGG - chr2.4:1940975-1940956 None:intergenic 25.0%
! TTGATCAAATAATCGCATAA+AGG + chr2.4:1941825-1941844 MS.gene89031:intron 25.0%
! TTGATCAATTACCTATAACA+TGG - chr2.4:1941813-1941832 None:intergenic 25.0%
!! ATTTAACTTATCTAAAAGCG+AGG - chr2.4:1942468-1942487 None:intergenic 25.0%
!! CAATGTTATATTGGTTAAAG+TGG - chr2.4:1941590-1941609 None:intergenic 25.0%
!! CAATTAACTTGATGATACAT+TGG + chr2.4:1942265-1942284 MS.gene89031:intron 25.0%
!! CTATAGTATGTATTTTGCAA+AGG - chr2.4:1943017-1943036 None:intergenic 25.0%
!! TAGAGTTTTTGTATCTGAAT+AGG - chr2.4:1941918-1941937 None:intergenic 25.0%
!! TTCTATTGTATTTCTGAACA+GGG + chr2.4:1943934-1943953 MS.gene89031:intron 25.0%
!! TTTCTATTGTATTTCTGAAC+AGG + chr2.4:1943933-1943952 MS.gene89031:intron 25.0%
!! TTTGAGGCAACTAAATAAAA+TGG - chr2.4:1940586-1940605 None:intergenic 25.0%
!!! AGTTCACAAAAGTACTAAAT+AGG - chr2.4:1942642-1942661 None:intergenic 25.0%
!!! ATGTTTTGAAAGTTTCAAGT+TGG + chr2.4:1940697-1940716 MS.gene89031:intron 25.0%
!!! CGTTTTAAAAGTTTTCAAGT+TGG - chr2.4:1940983-1941002 None:intergenic 25.0%
!!! GAATATTCCGATTTTGTTTT+AGG - chr2.4:1942689-1942708 None:intergenic 25.0%
!!! GTCACTTTTTGCTATATAAT+TGG - chr2.4:1942384-1942403 None:intergenic 25.0%
!!! TAACAAAGACATAATTTGCT+AGG + chr2.4:1942102-1942121 MS.gene89031:intron 25.0%
!!! TGCTATCGATTTCAAATAAA+AGG + chr2.4:1943140-1943159 MS.gene89031:CDS 25.0%
!!! TTCTTCTAAAGTATACTATG+TGG + chr2.4:1941642-1941661 MS.gene89031:intron 25.0%
AAAGGTTGTTACACAAAAGA+AGG + chr2.4:1941843-1941862 MS.gene89031:intron 30.0%
AACCAATCCATGTGTTTATT+TGG - chr2.4:1942718-1942737 None:intergenic 30.0%
ACCAATCCATGTGTTTATTT+GGG - chr2.4:1942717-1942736 None:intergenic 30.0%
AGACCAAAAGTTACAGATAT+AGG + chr2.4:1941683-1941702 MS.gene89031:intron 30.0%
AGAGCTATAAATACCACATT+TGG + chr2.4:1941873-1941892 MS.gene89031:intron 30.0%
ATATCAATGCCATCAGTATT+AGG - chr2.4:1943263-1943282 None:intergenic 30.0%
ATTGATCTTTCCATTAATGG+CGG - chr2.4:1942029-1942048 None:intergenic 30.0%
CATAAACACGATCAAATACA+AGG - chr2.4:1944296-1944315 None:intergenic 30.0%
CTAAAATACTAGTCAACTAG+AGG - chr2.4:1942567-1942586 None:intergenic 30.0%
CTTGAAACTTTCAAAACGTA+AGG + chr2.4:1940955-1940974 MS.gene89031:intron 30.0%
CTTGAAACTTTCAAAACGTA+AGG - chr2.4:1940974-1940955 None:intergenic 30.0%
GACCAAAAGTTACAGATATA+GGG + chr2.4:1941684-1941703 MS.gene89031:intron 30.0%
GAGCTATAAATACCACATTT+GGG + chr2.4:1941874-1941893 MS.gene89031:intron 30.0%
GATCAATTACCTATAACATG+GGG - chr2.4:1941811-1941830 None:intergenic 30.0%
TATAGAAATTTACTTACGGG+TGG - chr2.4:1942833-1942852 None:intergenic 30.0%
TATCAATGCCATCAGTATTA+GGG - chr2.4:1943262-1943281 None:intergenic 30.0%
TCCAAAAATTTCACTTGATG+AGG - chr2.4:1941319-1941338 None:intergenic 30.0%
TGAGTGATGTAACATATAGA+GGG + chr2.4:1944202-1944221 MS.gene89031:CDS 30.0%
TGTTGCACCTAAAACAAAAT+CGG + chr2.4:1942679-1942698 MS.gene89031:CDS 30.0%
TTAAGGCTTAATTGCATGTT+TGG + chr2.4:1940637-1940656 MS.gene89031:intron 30.0%
TTAATTTACAGGGGAATCAT+TGG - chr2.4:1940550-1940569 None:intergenic 30.0%
TTCAACCAAAATGCATTGAA+AGG + chr2.4:1941400-1941419 MS.gene89031:intron 30.0%
! ACCTCATCAAGTGAAATTTT+TGG + chr2.4:1941315-1941334 MS.gene89031:intron 30.0%
! ATTGGTTTCTCATTTGTGAA+TGG + chr2.4:1942731-1942750 MS.gene89031:CDS 30.0%
! GAACTTGAGACTTTGTTTAA+GGG + chr2.4:1941549-1941568 MS.gene89031:intron 30.0%
! TGAACTTGAGACTTTGTTTA+AGG + chr2.4:1941548-1941567 MS.gene89031:intron 30.0%
! TGGTATTTATAGCTCTTTTC+TGG - chr2.4:1941869-1941888 None:intergenic 30.0%
!! GTTTTAGTCTGTTAACTATG+TGG + chr2.4:1942175-1942194 MS.gene89031:intron 30.0%
!! TTTTGTAGCATTGGATCATT+GGG + chr2.4:1943645-1943664 MS.gene89031:intron 30.0%
!! TTTTTGTAGCATTGGATCAT+TGG + chr2.4:1943644-1943663 MS.gene89031:intron 30.0%
!!! ACGTTTTGAAAGTTTCAAGT+TGG - chr2.4:1940776-1940757 None:intergenic 30.0%
!!! CAAGTGAAATTTTTGGAACT+TGG + chr2.4:1941322-1941341 MS.gene89031:intron 30.0%
!!! TGAAATTTTTGGAACTTGGT+CGG + chr2.4:1941326-1941345 MS.gene89031:intron 30.0%
!!! TTTTGGTCTTATCCGAATTA+CGG - chr2.4:1941672-1941691 None:intergenic 30.0%
AAATGATTCACCGCCATTAA+TGG + chr2.4:1942016-1942035 MS.gene89031:intron 35.0%
AACACGATCAAATACAAGGT+TGG - chr2.4:1944292-1944311 None:intergenic 35.0%
AACTCTAACAAGCTGCAAAA+AGG + chr2.4:1943196-1943215 MS.gene89031:CDS 35.0%
AAGGAGAAAACCTTCATCTT+AGG - chr2.4:1944333-1944352 None:intergenic 35.0%
AAGTACTTGAATGTCTCTTG+AGG - chr2.4:1943291-1943310 None:intergenic 35.0%
ACCAAAATGCATTGAAAGGT+TGG + chr2.4:1941404-1941423 MS.gene89031:intron 35.0%
ACTCTAACAAGCTGCAAAAA+GGG + chr2.4:1943197-1943216 MS.gene89031:CDS 35.0%
AGTAAGAATCAAGACAAAGC+AGG + chr2.4:1943740-1943759 MS.gene89031:CDS 35.0%
AGTCTCAAGTTCAAACATTG+TGG - chr2.4:1941541-1941560 None:intergenic 35.0%
ATCTGAATAGGTCATGTCTT+GGG - chr2.4:1941906-1941925 None:intergenic 35.0%
ATGCACATCCTCTACAATAT+CGG - chr2.4:1943685-1943704 None:intergenic 35.0%
CAAAGGATCAATACAAGAGT+AGG - chr2.4:1943000-1943019 None:intergenic 35.0%
CGTACTACTACTCCTATATA+AGG + chr2.4:1943832-1943851 MS.gene89031:intron 35.0%
CTATATGTACCCCATGTTAT+AGG + chr2.4:1941799-1941818 MS.gene89031:intron 35.0%
CTTAACTCTTTCATTGCAAC+AGG + chr2.4:1943308-1943327 MS.gene89031:CDS 35.0%
GTAACATATAGAGGGATTAG+TGG + chr2.4:1944210-1944229 MS.gene89031:CDS 35.0%
GTGAGTGATGTAACATATAG+AGG + chr2.4:1944201-1944220 MS.gene89031:CDS 35.0%
GTTAGGCTTAATTGCATGTT+TGG - chr2.4:1941044-1941063 None:intergenic 35.0%
TATCTGAATAGGTCATGTCT+TGG - chr2.4:1941907-1941926 None:intergenic 35.0%
TCAAATGGTCTGCAAAAACA+TGG + chr2.4:1944050-1944069 MS.gene89031:CDS 35.0%
TCCCAAATAAACACATGGAT+TGG + chr2.4:1942713-1942732 MS.gene89031:CDS 35.0%
TCTGTTAACTATGTGGTCTA+TGG + chr2.4:1942182-1942201 MS.gene89031:intron 35.0%
TGGAACATCATTAACAGACA+AGG + chr2.4:1944230-1944249 MS.gene89031:CDS 35.0%
TTGTGTGTGTATGGATGTAT+AGG + chr2.4:1943100-1943119 MS.gene89031:intron 35.0%
TTTGAGAGATAAGGATGAGA+GGG - chr2.4:1941733-1941752 None:intergenic 35.0%
! AATAGGCTTTTCTCAAAGCA+TGG + chr2.4:1941134-1941153 MS.gene89031:intron 35.0%
! ACGTTGACTGAAACATTAAC+TGG + chr2.4:1943717-1943736 MS.gene89031:CDS 35.0%
! CAAGCAGTTTAGAATTGGTT+TGG - chr2.4:1944388-1944407 None:intergenic 35.0%
! GTCAATCATAATGGGGTTAT+GGG - chr2.4:1944013-1944032 None:intergenic 35.0%
! TATTGTATTTCTGAACAGGG+TGG + chr2.4:1943937-1943956 MS.gene89031:intron 35.0%
! TCCAACCTTTCAATGCATTT+TGG - chr2.4:1941408-1941427 None:intergenic 35.0%
! TTGAGCAAGCAGTTTAGAAT+TGG - chr2.4:1944393-1944412 None:intergenic 35.0%
! TTTGTAGCATTGGATCATTG+GGG + chr2.4:1943646-1943665 MS.gene89031:intron 35.0%
! TTTTGTGAACTCAGCTTTCA+GGG + chr2.4:1942651-1942670 MS.gene89031:intron 35.0%
!! TAATTTTGGTCACATGACGT+AGG + chr2.4:1943871-1943890 MS.gene89031:intron 35.0%
!! TGCAGCTTGTTAGAGTTATA+TGG - chr2.4:1943193-1943212 None:intergenic 35.0%
!! TTTTGAGAGATAAGGATGAG+AGG - chr2.4:1941734-1941753 None:intergenic 35.0%
!!! ACATGGAGAAGAGTATTTTG+AGG - chr2.4:1940602-1940621 None:intergenic 35.0%
!!! CAAGTTGGTCTCTTATGTTT+TGG + chr2.4:1940865-1940884 MS.gene89031:intron 35.0%
!!! TCTGGTTGTTTTGAGAGATA+AGG - chr2.4:1941742-1941761 None:intergenic 35.0%
AATAGAGAAGCTCAGTTGGA+AGG - chr2.4:1941954-1941973 None:intergenic 40.0%
AATTAGGTTTGCATGGACCA+CGG - chr2.4:1941243-1941262 None:intergenic 40.0%
AGAAATTTACTTACGGGTGG+TGG - chr2.4:1942830-1942849 None:intergenic 40.0%
AGAGTTATATGGCTTCTTGC+TGG - chr2.4:1943182-1943201 None:intergenic 40.0%
ATCTGCTGTTCCTAAGATGA+AGG + chr2.4:1944320-1944339 MS.gene89031:CDS 40.0%
CAGGATCCCAAATAAACACA+TGG + chr2.4:1942708-1942727 MS.gene89031:CDS 40.0%
CTAGAGGTTGTTCTACTTTC+TGG - chr2.4:1942551-1942570 None:intergenic 40.0%
CTGTTACGTACCATATGAAC+TGG + chr2.4:1943068-1943087 MS.gene89031:intron 40.0%
GAGTAGGTACTACTTACTGT+TGG - chr2.4:1942984-1943003 None:intergenic 40.0%
GATGTGTGTCAAAGCAAATC+AGG + chr2.4:1941160-1941179 MS.gene89031:CDS 40.0%
GTACAATAGAGAAGCTCAGT+TGG - chr2.4:1941958-1941977 None:intergenic 40.0%
GTATTGCAATATGGAGCAGT+TGG + chr2.4:1940513-1940532 MS.gene89031:CDS 40.0%
TAGGTACTACTTACTGTTGG+TGG - chr2.4:1942981-1943000 None:intergenic 40.0%
TTGAACTGCGACCAAAATGT+GGG + chr2.4:1944261-1944280 MS.gene89031:CDS 40.0%
TTTGAACTGCGACCAAAATG+TGG + chr2.4:1944260-1944279 MS.gene89031:CDS 40.0%
! CGCCCTATATCTGTAACTTT+TGG - chr2.4:1941689-1941708 None:intergenic 40.0%
! CTTTTGTGAACTCAGCTTTC+AGG + chr2.4:1942650-1942669 MS.gene89031:intron 40.0%
! GATTGCATTGCTATTAGTGC+TGG + chr2.4:1943441-1943460 MS.gene89031:CDS 40.0%
! GGTCAATCATAATGGGGTTA+TGG - chr2.4:1944014-1944033 None:intergenic 40.0%
! TACTACTTACTGTTGGTGGA+CGG - chr2.4:1942977-1942996 None:intergenic 40.0%
! TTGTAGCATTGGATCATTGG+GGG + chr2.4:1943647-1943666 MS.gene89031:intron 40.0%
!! TAGAATTGGTTTGGTGTGTG+AGG - chr2.4:1944379-1944398 None:intergenic 40.0%
!!! AATTTTTGGAACTTGGTCGG+TGG + chr2.4:1941329-1941348 MS.gene89031:intron 40.0%
AACATCCGTCTAATTGCTCC+TGG + chr2.4:1943230-1943249 MS.gene89031:CDS 45.0%
ACTGCTATAACATGTGGTCC+AGG + chr2.4:1943483-1943502 MS.gene89031:CDS 45.0%
AGGCTCTTCCATGAGCATTA+TGG - chr2.4:1944359-1944378 None:intergenic 45.0%
AGTAAGAATGTCTTGGCTGC+TGG - chr2.4:1941205-1941224 None:intergenic 45.0%
ATAACATGTGGTCCAGGACA+TGG + chr2.4:1943489-1943508 MS.gene89031:CDS 45.0%
CAATATGGAGCAGTTGGAGA+TGG + chr2.4:1940519-1940538 MS.gene89031:CDS 45.0%
CAGCCAAGACATTCTTACTG+AGG + chr2.4:1941206-1941225 MS.gene89031:CDS 45.0%
CTTGGGAATGATCCCAAATG+TGG - chr2.4:1941889-1941908 None:intergenic 45.0%
TCCATGAGCATTATGGCAGA+AGG - chr2.4:1944352-1944371 None:intergenic 45.0%
TCCTTCTGCCATAATGCTCA+TGG + chr2.4:1944348-1944367 MS.gene89031:CDS 45.0%
TCTCTCCAGGAGCAATTAGA+CGG - chr2.4:1943238-1943257 None:intergenic 45.0%
TGGAGACACCGATATTGTAG+AGG + chr2.4:1943674-1943693 MS.gene89031:CDS 45.0%
TGGCCTCAGTAAGAATGTCT+TGG - chr2.4:1941212-1941231 None:intergenic 45.0%
! GGCTTTTCTCAAAGCATGGA+AGG + chr2.4:1941138-1941157 MS.gene89031:intron 45.0%
! TGTATTTCTGAACAGGGTGG+AGG + chr2.4:1943940-1943959 MS.gene89031:intron 45.0%
!! TGGTCTTATCCGAATTACGG+CGG - chr2.4:1941669-1941688 None:intergenic 45.0%
ACCAACCTGATACCATGTCC+TGG - chr2.4:1943504-1943523 None:intergenic 50.0%
AGTGGAAAGGGGACTGTTGA+TGG + chr2.4:1942764-1942783 MS.gene89031:CDS 50.0%
ATTTCTGAACAGGGTGGAGG+TGG + chr2.4:1943943-1943962 MS.gene89031:intron 50.0%
CTATGTGGACCGCCGTAATT+CGG + chr2.4:1941657-1941676 MS.gene89031:intron 50.0%
GGAGAGAGCCCTAATACTGA+TGG + chr2.4:1943251-1943270 MS.gene89031:CDS 50.0%
TCAGTATTAGGGCTCTCTCC+AGG - chr2.4:1943251-1943270 None:intergenic 50.0%
TCCAGGACATGGTATCAGGT+TGG + chr2.4:1943500-1943519 MS.gene89031:intron 50.0%
TGGTGGAAACAACCGTGCAT+AGG + chr2.4:1942800-1942819 MS.gene89031:CDS 50.0%
! TTGATGGTCGAGGCTCTATG+TGG + chr2.4:1942780-1942799 MS.gene89031:CDS 50.0%
!! GGTGGTGGATTTCCTATGCA+CGG - chr2.4:1942815-1942834 None:intergenic 50.0%
!! GGTTGGAGCATCCCACATTT+TGG - chr2.4:1944275-1944294 None:intergenic 50.0%
AGGTTTGCATGGACCACGGA+AGG - chr2.4:1941239-1941258 None:intergenic 55.0%
ATGGTCGAGGCTCTATGTGG+TGG + chr2.4:1942783-1942802 MS.gene89031:CDS 55.0%
CATGGACCACGGAAGGCTAT+TGG - chr2.4:1941232-1941251 None:intergenic 55.0%
GAGGGCAGCCTCATGGATTT+AGG - chr2.4:1941715-1941734 None:intergenic 55.0%
GGCAGCCTCATGGATTTAGG+CGG - chr2.4:1941712-1941731 None:intergenic 55.0%
GTGGTCCAGGACATGGTATC+AGG + chr2.4:1943496-1943515 MS.gene89031:CDS 55.0%
! AGGGTGGAGGTGGATTTGCT+AGG + chr2.4:1943953-1943972 MS.gene89031:intron 55.0%
! ATTGGATCATTGGGGGCACG+TGG + chr2.4:1943654-1943673 MS.gene89031:CDS 55.0%
!! AAGGGGACTGTTGATGGTCG+AGG + chr2.4:1942770-1942789 MS.gene89031:CDS 55.0%
!! GTGGAGGTGGATTTGCTAGG+AGG + chr2.4:1943956-1943975 MS.gene89031:CDS 55.0%
AGGATGAGAGGGCAGCCTCA+TGG - chr2.4:1941722-1941741 None:intergenic 60.0%
CTGAGGCCAATAGCCTTCCG+TGG + chr2.4:1941223-1941242 MS.gene89031:CDS 60.0%
! GGGCGCCGCCTAAATCCATG+AGG + chr2.4:1941704-1941723 MS.gene89031:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 1940462 1944416 1940462 ID=MS.gene89031
chr2.4 mRNA 1940462 1944416 1940462 ID=MS.gene89031.t1;Parent=MS.gene89031
chr2.4 exon 1940462 1940560 1940462 ID=MS.gene89031.t1.exon1;Parent=MS.gene89031.t1
chr2.4 CDS 1940462 1940560 1940462 ID=cds.MS.gene89031.t1;Parent=MS.gene89031.t1
chr2.4 exon 1941139 1941276 1941139 ID=MS.gene89031.t1.exon2;Parent=MS.gene89031.t1
chr2.4 CDS 1941139 1941276 1941139 ID=cds.MS.gene89031.t1;Parent=MS.gene89031.t1
chr2.4 exon 1942665 1942835 1942665 ID=MS.gene89031.t1.exon3;Parent=MS.gene89031.t1
chr2.4 CDS 1942665 1942835 1942665 ID=cds.MS.gene89031.t1;Parent=MS.gene89031.t1
chr2.4 exon 1942963 1942986 1942963 ID=MS.gene89031.t1.exon4;Parent=MS.gene89031.t1
chr2.4 CDS 1942963 1942986 1942963 ID=cds.MS.gene89031.t1;Parent=MS.gene89031.t1
chr2.4 exon 1943122 1943329 1943122 ID=MS.gene89031.t1.exon5;Parent=MS.gene89031.t1
chr2.4 CDS 1943122 1943329 1943122 ID=cds.MS.gene89031.t1;Parent=MS.gene89031.t1
chr2.4 exon 1943436 1943517 1943436 ID=MS.gene89031.t1.exon6;Parent=MS.gene89031.t1
chr2.4 CDS 1943436 1943517 1943436 ID=cds.MS.gene89031.t1;Parent=MS.gene89031.t1
chr2.4 exon 1943653 1943761 1943653 ID=MS.gene89031.t1.exon7;Parent=MS.gene89031.t1
chr2.4 CDS 1943653 1943761 1943653 ID=cds.MS.gene89031.t1;Parent=MS.gene89031.t1
chr2.4 exon 1943955 1944071 1943955 ID=MS.gene89031.t1.exon8;Parent=MS.gene89031.t1
chr2.4 CDS 1943955 1944071 1943955 ID=cds.MS.gene89031.t1;Parent=MS.gene89031.t1
chr2.4 exon 1944186 1944416 1944186 ID=MS.gene89031.t1.exon9;Parent=MS.gene89031.t1
chr2.4 CDS 1944186 1944416 1944186 ID=cds.MS.gene89031.t1;Parent=MS.gene89031.t1
Gene Sequence

>MS.gene89031

ATGATTCTAAGTATGTTTAAACTCGGATTGAGTAGCACAACAACTTTCAATGTATTGCAATATGGAGCAGTTGGAGATGGAAAAACCAATGATTCCCCTGTAAATTAACTATGATCTCTCACCATTTTATTTAGTTGCCTCAAAATACTCTTCTCCATGTGTAACATTAATAACTTTAAGGCTTAATTGCATGTTTGGTCCCTTATGTTTATTTTAGTTTTCAAGTTGGTCCCTTATGTTTTGAAAGTTTCAAGTTGGTCCCATACGTTTATTTTAGTTTTCAAGTTGGTCCCTTACGTTTTGAAAGTTTCAAGTTAGTCCCTTATGTTTATTTTAGTTTTCAAGTTGGTCCCTTATGTTTATTTTAGTTTTCAAGTTGGACCCTTATGTTTATTTTAGTTTTCAAGTTGGTCTCTTATGTTTTGGACCGACTTGAAAACTAAAATAAACATAAGGGACCAACTTGAAAACTAAAATAAACATAAGGGACCAACTTGAAACTTTCAAAACGTAAGGGACCAACTTGAAAACTTTTAAAACGTAAGGGACCAACTTGAAAACTAAAATAAACATAAGGGACCAAACATGCAATTAAGCCTAACTTTAATTATTTCATTTGCTCAATTCCAAATGTGATAAAATAATTTAAAGTTTATGCTATAATATTTTTTCAATAGGCTTTTCTCAAAGCATGGAAGGATGTGTGTCAAAGCAAATCAGGCACATCTCGTCTCATCATACCAGCAGCCAAGACATTCTTACTGAGGCCAATAGCCTTCCGTGGTCCATGCAAACCTAATTATATATACATTGAGGTACTTATTAATTAATTTGATTATTTTTGCAATACACAACCTCATCAAGTGAAATTTTTGGAACTTGGTCGGTGGCTATAACTTAAAAGCATAGTAAACATTAACGTGAGTACTGTTTCAACTTTCAACCAAAATGCATTGAAAGGTTGGAATTTGATAAAATTGATATGTTATTTTAGTTTTTTATAACCATATTATTTCTGGCAAGTATTCACATTTAAGACTTAAGTTGATCTCACCTCTCAAGTATATTTTTTTCATCCACAATGTTTGAACTTGAGACTTTGTTTAAGGGATTCAAGTTTCAATTCCACTTTAACCAATATAACATTGGTAATTGATGTGTTACTTTATTTAGGATCTAGTTCTTCTAAAGTATACTATGTGGACCGCCGTAATTCGGATAAGACCAAAAGTTACAGATATAGGGCGCCGCCTAAATCCATGAGGCTGCCCTCTCATCCTTATCTCTCAAAACAACCAGATCATAATCTTTAATGTAGGATATTCAGACAGAGATGACTATATGTACCCCATGTTATAGGTAATTGATCAAATAATCGCATAAAGGTTGTTACACAAAAGAAGGCCAGAAAAGAGCTATAAATACCACATTTGGGATCATTCCCAAGACATGACCTATTCAGATACAAAAACTCTAATCATTCAGTTAACCTTCCAACTGAGCTTCTCTATTGTACTCACTTCAAGTAGACTATGAAAAGAAAAATGAGTTATGAAATGATTCACCGCCATTAATGGAAAGATCAATTAAGGGAATGCATTTTAAGTTTAACAAATTCATATTTGTAAAAGAAAATGGTTTAACAAAGACATAATTTGCTAGGAATGAAAAAAGTGATATCAACAAACATACATTGTGTCACAAATTGTCGATGTTTTAGTCTGTTAACTATGTGGTCTATGGTTCAATTTATTATCAAAGCATATGAAAAAACATACAAGAGATAAATTCTCAAATGGATATCAATTAACTTGATGATACATTGGTTTTAAAAAAAAATATATATATATTATACAGTGTCTTCAAGACATTGATTAAGCAAGCAAAACAAATAAGTAATTTTTTTATAAATTCCTTAACCAATTATATAGCAAAAAGTGACATGAGTGATGTTCATCATAAGTGAGTTTTCATTTTTTGGTATTTTCTATTCAAAAGTCAAACCTCGCTTTTAGATAAGTTAAATTCATTAAAAGTACGAATAGTTAAGTACTTTACAAAGGAATAAAAAAAGAACAAAAATAATCCAGAAAGTAGAACAACCTCTAGTTGACTAGTATTTTAGTAGTATAATTTTATTTGAATTAAATTGAGAACTAAGCTTTGAGTTAAAGTAACCTATTTAGTACTTTTGTGAACTCAGCTTTCAGGGAATATTGTTGCACCTAAAACAAAATCGGAATATTCAGGATCCCAAATAAACACATGGATTGGTTTCTCATTTGTGAATGGTTTAATTATAAGTGGAAAGGGGACTGTTGATGGTCGAGGCTCTATGTGGTGGAAACAACCGTGCATAGGAAATCCACCACCCGTAAGTAAATTTCTATATCTATAGTTTTTTTTAACTAAACATTTCAACTTATGTTACTTAAATAATACACTATATACCTTTATAGTAATACTTGCATCATTTTTATGATTTTTTTTTTCTTTTTCAGGGGACAAGTTGCCGTCCACCAACAGTAAGTAGTACCTACTCTTGTATTGATCCTTTGCAAAATACATACTATAGACAATATTTTTGTTACAAAATTAAATTTTAGCTGTTACGTACCATATGAACTGGTATTTATTTTTGTGTGTGTATGGATGTATAGGCAATAACATTGAATAGATGCTATCGATTTCAAATAAAAGGATATACAAGTGTTAATCCAGCAAGAAGCCATATAACTCTAACAAGCTGCAAAAAGGGCATTATCTCTAACATCCGTCTAATTGCTCCTGGAGAGAGCCCTAATACTGATGGCATTGATATTTCTGCCTCAAGAGACATTCAAGTACTTAACTCTTTCATTGCAACAGGTAATATATGTTTTAATTTGAACTCATTTCAATCTATATTATTAATTATTAGATCTTAGTGTATGTAAATAATCATATATATTGATTGTATCTAATCATATTATAGGTGATGATTGCATTGCTATTAGTGCTGGATCATCAGTAATCAAAATAACTGCTATAACATGTGGTCCAGGACATGGTATCAGGTTGGTAGTACTTCACTAAATATGTACTAATTGATCTGAACTTATAAAATATGATCTAATTGATCTGAACTAATTATATCATCCATTAATCTAGCAACTTTACATTAATTAAGAAAATATATATTATTTTTGTAGCATTGGATCATTGGGGGCACGTGGAGACACCGATATTGTAGAGGATGTGCATGTGAAGAATTGTACGTTGACTGAAACATTAACTGGAGTAAGAATCAAGACAAAGCAGGTAGTAATTAATTCACCAAAATAACTAACATTATTGGTTACAAACTCCATGAGTATTAATTTCTTTATTACGTACTACTACTCCTATATAAGGTTTTTCACTTATACTATAATTTTGGTCACATGACGTAGGTAAATTATTTTATTTTAAATTTTAAATGTAATCATTCAATTTCTATTGTATTTCTGAACAGGGTGGAGGTGGATTTGCTAGGAGGATCACGTTTGAAAATATTAGATTTGTTCGAGCCCATAACCCCATTATGATTGACCAATTTTATTGTGTTAATCAAATGGTCTGCAAAAACATGGTAACTCAAATAAATCATCAATTCCATTTTTCTTATATATGTTACTCCCCAAAAAATAAACTAAATTTCTACAACTTGCATTGTAACAATTTTTTCATTGAATGTTATGTGTAGACTAAAGCAATCAAAGTGAGTGATGTAACATATAGAGGGATTAGTGGAACATCATTAACAGACAAGGCAATAAATTTGAACTGCGACCAAAATGTGGGATGCTCCAACCTTGTATTTGATCGTGTTTATGTAAGATCTGCTGTTCCTAAGATGAAGGTTTTCTCCTTCTGCCATAATGCTCATGGAAGAGCCTCACACACCAAACCAATTCTAAACTGCTTGCTCAAGTAG
Protein sequence

>MS.gene89031.t1

MILSMFKLGLSSTTTFNVLQYGAVGDGKTNDSPAFLKAWKDVCQSKSGTSRLIIPAAKTFLLRPIAFRGPCKPNYIYIELSGNIVAPKTKSEYSGSQINTWIGFSFVNGLIISGKGTVDGRGSMWWKQPCIGNPPPGTSCRPPTAITLNRCYRFQIKGYTSVNPARSHITLTSCKKGIISNIRLIAPGESPNTDGIDISASRDIQVLNSFIATGDDCIAISAGSSVIKITAITCGPGHGISIGSLGARGDTDIVEDVHVKNCTLTETLTGVRIKTKQGGGGFARRITFENIRFVRAHNPIMIDQFYCVNQMVCKNMTKAIKVSDVTYRGISGTSLTDKAINLNCDQNVGCSNLVFDRVYVRSAVPKMKVFSFCHNAHGRASHTKPILNCLLK