Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89265.t1 | XP_003623166.1 | 97.4 | 117 | 3 | 0 | 1 | 117 | 1 | 117 | 1.40E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89265.t1 | G7L5L7 | 97.4 | 117 | 3 | 0 | 1 | 117 | 1 | 117 | 1.0e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051552 | MS.gene89265 | 0.804019 | 2.64E-49 | -1.69E-46 |
| MS.gene053776 | MS.gene89265 | 0.808804 | 2.59E-50 | -1.69E-46 |
| MS.gene055158 | MS.gene89265 | 0.920235 | 1.65E-87 | -1.69E-46 |
| MS.gene055159 | MS.gene89265 | 0.867915 | 9.62E-66 | -1.69E-46 |
| MS.gene058158 | MS.gene89265 | 0.800389 | 1.47E-48 | -1.69E-46 |
| MS.gene058418 | MS.gene89265 | 0.826328 | 2.88E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89265.t1 | MTR_7g065210 | 97.436 | 117 | 3 | 0 | 1 | 117 | 1 | 117 | 3.73e-82 | 236 |
| MS.gene89265.t1 | MTR_7g065160 | 88.034 | 117 | 14 | 0 | 1 | 117 | 1 | 117 | 1.34e-74 | 217 |
| MS.gene89265.t1 | MTR_8g070040 | 51.515 | 99 | 46 | 2 | 20 | 117 | 23 | 120 | 1.11e-25 | 93.6 |
| MS.gene89265.t1 | MTR_8g070015 | 47.826 | 92 | 46 | 2 | 20 | 110 | 23 | 113 | 4.82e-20 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89265.t1 | AT3G53980 | 68.421 | 95 | 29 | 1 | 23 | 117 | 21 | 114 | 1.31e-44 | 141 |
| MS.gene89265.t1 | AT3G53980 | 68.421 | 95 | 29 | 1 | 23 | 117 | 21 | 114 | 1.31e-44 | 141 |
| MS.gene89265.t1 | AT5G05960 | 58.772 | 114 | 40 | 2 | 4 | 117 | 10 | 116 | 1.41e-42 | 136 |
| MS.gene89265.t1 | AT2G37870 | 45.370 | 108 | 57 | 2 | 11 | 117 | 9 | 115 | 9.88e-26 | 93.6 |
Find 22 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTAAATGCTTCCATATC+TGG | 0.265044 | 7.1:-37097167 | None:intergenic |
| TAGCAAAGTTACAGCGTTTA+GGG | 0.295883 | 7.1:-37097352 | None:intergenic |
| TTAGCAAAGTTACAGCGTTT+AGG | 0.343174 | 7.1:-37097353 | None:intergenic |
| CGTCCGCACTTATAACCAAT+GGG | 0.457526 | 7.1:-37097380 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.462464 | 7.1:+37097302 | MS.gene89265:CDS |
| AAGGGGTACTACACCAGATA+TGG | 0.470603 | 7.1:+37097154 | MS.gene89265:CDS |
| GGATAGTAACAGCAATTTGA+GGG | 0.472152 | 7.1:-37097331 | None:intergenic |
| ATGAAGATGCTGTGCCTAGT+TGG | 0.489536 | 7.1:+37097077 | MS.gene89265:CDS |
| GGGATAGTAACAGCAATTTG+AGG | 0.508534 | 7.1:-37097332 | None:intergenic |
| ACGTCCGCACTTATAACCAA+TGG | 0.512832 | 7.1:-37097381 | None:intergenic |
| GTTGAAAGTGCAGGTGAATG+TGG | 0.515102 | 7.1:+37097131 | MS.gene89265:CDS |
| AACTTTGCTAATCGCCCCAT+TGG | 0.524057 | 7.1:+37097365 | MS.gene89265:CDS |
| TCTTGTGCTGCACTTGCACA+AGG | 0.541464 | 7.1:-37097194 | None:intergenic |
| AAAGTGCAGGTGAATGTGGA+AGG | 0.549386 | 7.1:+37097135 | MS.gene89265:CDS |
| AAAACTGCACAAAGACAACT+TGG | 0.590774 | 7.1:-37097275 | None:intergenic |
| ATTCATAGAGTTGAAAGTGC+AGG | 0.591870 | 7.1:+37097122 | MS.gene89265:CDS |
| TGTGCTCAGACTAAGAAACT+TGG | 0.591888 | 7.1:+37097245 | MS.gene89265:CDS |
| CAAGAACTACAAAACCAACT+AGG | 0.604153 | 7.1:-37097091 | None:intergenic |
| CGCCCCATTGGTTATAAGTG+CGG | 0.627824 | 7.1:+37097377 | MS.gene89265:CDS |
| AGTGCAGGTGAATGTGGAAG+GGG | 0.630405 | 7.1:+37097137 | MS.gene89265:CDS |
| AAGTGCAGGTGAATGTGGAA+GGG | 0.641454 | 7.1:+37097136 | MS.gene89265:CDS |
| GTCCGCACTTATAACCAATG+GGG | 0.757650 | 7.1:-37097379 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAAAAAAATAACACG+TGG | + | chr7.1:37097765-37097784 | MS.gene89265:intron | 15.0% |
| !! | AAGAATAAATATAAACGAAA+TGG | - | chr7.1:37097460-37097479 | None:intergenic | 15.0% |
| !!! | ATTTTGTAGTTTTTTTTCTT+AGG | - | chr7.1:37097674-37097693 | None:intergenic | 15.0% |
| !!! | TTTAGTAATTTTTTTTCCTT+AGG | + | chr7.1:37097878-37097897 | MS.gene89265:intron | 15.0% |
| !!! | TTTTATTTTAAAATTCATGC+AGG | - | chr7.1:37097754-37097773 | None:intergenic | 15.0% |
| !!! | TTTTGTAGTTTTTTTTCTTA+GGG | - | chr7.1:37097673-37097692 | None:intergenic | 15.0% |
| !! | AACAACAAAAAAATGTGAAA+CGG | + | chr7.1:37097971-37097990 | MS.gene89265:intron | 20.0% |
| !!! | CTATTTGTTAATTTTCTTGA+TGG | + | chr7.1:37098031-37098050 | MS.gene89265:intron | 20.0% |
| !!! | TTTGTAGTTTTTTTTCTTAG+GGG | - | chr7.1:37097672-37097691 | None:intergenic | 20.0% |
| !!! | AGTAATTTTTTTTCCTTAGG+AGG | + | chr7.1:37097881-37097900 | MS.gene89265:intron | 25.0% |
| !!! | TTGTAGTTTTTTTTCTTAGG+GGG | - | chr7.1:37097671-37097690 | None:intergenic | 25.0% |
| GACAACTTAAAAGAAACTGA+GGG | - | chr7.1:37097422-37097441 | None:intergenic | 30.0% | |
| ! | AATCGCAACTTTGAAAAGTT+AGG | + | chr7.1:37097908-37097927 | MS.gene89265:intron | 30.0% |
| ! | ATCGCAACTTTGAAAAGTTA+GGG | + | chr7.1:37097909-37097928 | MS.gene89265:intron | 30.0% |
| AAAACTGCACAAAGACAACT+TGG | - | chr7.1:37097278-37097297 | None:intergenic | 35.0% | |
| AGCTTAAATGCTTCCATATC+TGG | - | chr7.1:37097170-37097189 | None:intergenic | 35.0% | |
| CAAGAACTACAAAACCAACT+AGG | - | chr7.1:37097094-37097113 | None:intergenic | 35.0% | |
| CGACAACTTAAAAGAAACTG+AGG | - | chr7.1:37097423-37097442 | None:intergenic | 35.0% | |
| CTAAAATCACAACTTCGTGA+AGG | - | chr7.1:37097648-37097667 | None:intergenic | 35.0% | |
| CTACAGTTGCAAAACTTAGA+GGG | - | chr7.1:37097699-37097718 | None:intergenic | 35.0% | |
| GGATAGTAACAGCAATTTGA+GGG | - | chr7.1:37097334-37097353 | None:intergenic | 35.0% | |
| TAGCAAAGTTACAGCGTTTA+GGG | - | chr7.1:37097355-37097374 | None:intergenic | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | + | chr7.1:37097302-37097321 | MS.gene89265:CDS | 35.0% | |
| TCATGCAGGTGCAAAAAAAT+TGG | - | chr7.1:37097740-37097759 | None:intergenic | 35.0% | |
| TTAGCAAAGTTACAGCGTTT+AGG | - | chr7.1:37097356-37097375 | None:intergenic | 35.0% | |
| ! | ATTCATAGAGTTGAAAGTGC+AGG | + | chr7.1:37097122-37097141 | MS.gene89265:CDS | 35.0% |
| ! | GTTTTGTAGTTCTTGTTGCA+GGG | + | chr7.1:37097099-37097118 | MS.gene89265:CDS | 35.0% |
| ! | TTTTGCAACTGTAGCAGTTT+TGG | + | chr7.1:37097705-37097724 | MS.gene89265:intron | 35.0% |
| !! | GTGCAAAAAAATTGGCCTTT+GGG | - | chr7.1:37097732-37097751 | None:intergenic | 35.0% |
| !! | TGCAAAAAAATTGGCCTTTG+GGG | - | chr7.1:37097731-37097750 | None:intergenic | 35.0% |
| ATCACAACTTCGTGAAGGTT+AGG | - | chr7.1:37097643-37097662 | None:intergenic | 40.0% | |
| GCTACAGTTGCAAAACTTAG+AGG | - | chr7.1:37097700-37097719 | None:intergenic | 40.0% | |
| GGGATAGTAACAGCAATTTG+AGG | - | chr7.1:37097335-37097354 | None:intergenic | 40.0% | |
| TCACAACTTCGTGAAGGTTA+GGG | - | chr7.1:37097642-37097661 | None:intergenic | 40.0% | |
| TGTGCTCAGACTAAGAAACT+TGG | + | chr7.1:37097245-37097264 | MS.gene89265:CDS | 40.0% | |
| ! | GCAACTTTGAAAAGTTAGGG+AGG | + | chr7.1:37097912-37097931 | MS.gene89265:intron | 40.0% |
| !! | GGTGCAAAAAAATTGGCCTT+TGG | - | chr7.1:37097733-37097752 | None:intergenic | 40.0% |
| !! | GGTTTTGTAGTTCTTGTTGC+AGG | + | chr7.1:37097098-37097117 | MS.gene89265:CDS | 40.0% |
| AAAGTGCAGGTGAATGTGGA+AGG | + | chr7.1:37097135-37097154 | MS.gene89265:CDS | 45.0% | |
| AACACGTGGCACCTCATATA+GGG | + | chr7.1:37097779-37097798 | MS.gene89265:intron | 45.0% | |
| AACTTTGCTAATCGCCCCAT+TGG | + | chr7.1:37097365-37097384 | MS.gene89265:CDS | 45.0% | |
| AAGTGCAGGTGAATGTGGAA+GGG | + | chr7.1:37097136-37097155 | MS.gene89265:CDS | 45.0% | |
| ACAACTTCGTGAAGGTTAGG+GGG | - | chr7.1:37097640-37097659 | None:intergenic | 45.0% | |
| ACGTCCGCACTTATAACCAA+TGG | - | chr7.1:37097384-37097403 | None:intergenic | 45.0% | |
| AGCGTAGACCGAGTCAAAAT+TGG | + | chr7.1:37097811-37097830 | MS.gene89265:intron | 45.0% | |
| ATGAAGATGCTGTGCCTAGT+TGG | + | chr7.1:37097077-37097096 | MS.gene89265:CDS | 45.0% | |
| CACAACTTCGTGAAGGTTAG+GGG | - | chr7.1:37097641-37097660 | None:intergenic | 45.0% | |
| CGTCCGCACTTATAACCAAT+GGG | - | chr7.1:37097383-37097402 | None:intergenic | 45.0% | |
| GTCCGCACTTATAACCAATG+GGG | - | chr7.1:37097382-37097401 | None:intergenic | 45.0% | |
| TAACACGTGGCACCTCATAT+AGG | + | chr7.1:37097778-37097797 | MS.gene89265:intron | 45.0% | |
| ! | GTTGAAAGTGCAGGTGAATG+TGG | + | chr7.1:37097131-37097150 | MS.gene89265:CDS | 45.0% |
| !! | AAGGGGTACTACACCAGATA+TGG | + | chr7.1:37097154-37097173 | MS.gene89265:CDS | 45.0% |
| !! | ACCGAGTCAAAATTGGCCTT+GGG | + | chr7.1:37097818-37097837 | MS.gene89265:intron | 45.0% |
| !! | GTTGCGATTTTGACCTCCTA+AGG | - | chr7.1:37097897-37097916 | None:intergenic | 45.0% |
| !! | TATATTAATATATGAAAAAA+AGG | + | chr7.1:37097597-37097616 | MS.gene89265:intron | 5.0% |
| AGTGCAGGTGAATGTGGAAG+GGG | + | chr7.1:37097137-37097156 | MS.gene89265:CDS | 50.0% | |
| CCACCGGTGCAAAACTTAGA+GGG | + | chr7.1:37097852-37097871 | MS.gene89265:intron | 50.0% | |
| CTGATGTGGCACCCTATATG+AGG | - | chr7.1:37097793-37097812 | None:intergenic | 50.0% | |
| TCTTGTGCTGCACTTGCACA+AGG | - | chr7.1:37097197-37097216 | None:intergenic | 50.0% | |
| ! | CGCCCCATTGGTTATAAGTG+CGG | + | chr7.1:37097377-37097396 | MS.gene89265:CDS | 50.0% |
| ! | GACCGAGTCAAAATTGGCCT+TGG | + | chr7.1:37097817-37097836 | MS.gene89265:intron | 50.0% |
| !! | CCGAGTCAAAATTGGCCTTG+GGG | + | chr7.1:37097819-37097838 | MS.gene89265:intron | 50.0% |
| !! | CGAGTCAAAATTGGCCTTGG+GGG | + | chr7.1:37097820-37097839 | MS.gene89265:intron | 50.0% |
| !! | TGTAGCAGTTTTGGCCCCAA+AGG | + | chr7.1:37097714-37097733 | MS.gene89265:intron | 50.0% |
| !! | TTTTGCACCGGTGGCAGTTT+TGG | - | chr7.1:37097846-37097865 | None:intergenic | 50.0% |
| !!! | CCCCAAGGCCAATTTTGACT+CGG | - | chr7.1:37097822-37097841 | None:intergenic | 50.0% |
| CCGGTGCAAAACTTAGAGGG+CGG | + | chr7.1:37097855-37097874 | MS.gene89265:intron | 55.0% | |
| GCCACCGGTGCAAAACTTAG+AGG | + | chr7.1:37097851-37097870 | MS.gene89265:intron | 55.0% | |
| ! | CCCTCTAAGTTTTGCACCGG+TGG | - | chr7.1:37097855-37097874 | None:intergenic | 55.0% |
| ! | CCGCCCTCTAAGTTTTGCAC+CGG | - | chr7.1:37097858-37097877 | None:intergenic | 55.0% |
| !! | TGACTCGGTCTACGCTGATG+TGG | - | chr7.1:37097807-37097826 | None:intergenic | 55.0% |
| TTGGGGGCCAAAACTGCCAC+CGG | + | chr7.1:37097836-37097855 | MS.gene89265:intron | 60.0% | |
| !! | GGTGGCAGTTTTGGCCCCCA+AGG | - | chr7.1:37097837-37097856 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 37097065 | 37098111 | 37097065 | ID=MS.gene89265 |
| chr7.1 | mRNA | 37097065 | 37098111 | 37097065 | ID=MS.gene89265.t1;Parent=MS.gene89265 |
| chr7.1 | exon | 37097065 | 37097401 | 37097065 | ID=MS.gene89265.t1.exon1;Parent=MS.gene89265.t1 |
| chr7.1 | CDS | 37097065 | 37097401 | 37097065 | ID=cds.MS.gene89265.t1;Parent=MS.gene89265.t1 |
| chr7.1 | exon | 37098095 | 37098111 | 37098095 | ID=MS.gene89265.t1.exon2;Parent=MS.gene89265.t1 |
| chr7.1 | CDS | 37098095 | 37098111 | 37098095 | ID=cds.MS.gene89265.t1;Parent=MS.gene89265.t1 |
| Gene Sequence |
| Protein sequence |
