AlfalfaGEDB | MS.gene89612

M. sativa cultivar XinJiangDaYe / MS.gene89612

KEGG: K02935
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00542.22 PF16320.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89612.t1	XP_003629385.1	90.8	196	17	1	1	196	1	195	4.20E-76	294.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89612.t1	P02398	36.7	166	81	3	33	195	44	188	2.5e-16	87.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89612.t1	G7LD30	90.8	196	17	1	1	196	1	195	3.1e-76	294.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052376	MS.gene89612	0.801903	7.23E-49	-1.69E-46
MS.gene060480	MS.gene89612	0.803735	3.03E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene89612	MS.gene22417	PPI
MS.gene36292	MS.gene89612	PPI
MS.gene89612	MS.gene89107	PPI
MS.gene007416	MS.gene89612	PPI
MS.gene89612	MS.gene61205	PPI
MS.gene89612	MS.gene007531	PPI
MS.gene20079	MS.gene89612	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89612.t1	MTR_8g076780	90.816	196	17	1	1	196	1	195	7.69e-108	307
MS.gene89612.t1	MTR_7g053520	50.000	148	64	3	50	196	79	217	7.21e-33	117
MS.gene89612.t1	MTR_2g031960	45.513	156	70	2	43	196	13	155	4.08e-32	114
MS.gene89612.t1	MTR_2g064300	48.299	147	68	2	50	196	78	216	1.89e-31	114
MS.gene89612.t1	MTR_2g031760	46.250	160	69	4	41	196	44	190	1.18e-30	111
MS.gene89612.t1	MTR_6g006060	33.824	136	72	1	60	195	63	180	1.26e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89612.t1	AT4G36420	51.613	186	77	4	17	196	1	179	7.58e-50	159
MS.gene89612.t1	AT1G70190	48.649	148	72	1	49	196	65	208	3.12e-33	118
MS.gene89612.t1	AT1G70190	48.649	148	72	1	49	196	65	208	3.12e-33	118
MS.gene89612.t1	AT3G06040	63.441	93	30	1	104	196	98	186	3.11e-29	107
MS.gene89612.t1	AT3G06040	63.441	93	30	1	104	196	98	186	3.11e-29	107
MS.gene89612.t1	AT3G06040	63.441	93	30	1	104	196	98	186	3.11e-29	107
MS.gene89612.t1	AT4G37660	42.857	161	74	3	38	196	23	167	4.90e-25	95.9
MS.gene89612.t1	AT3G27850	38.194	144	73	1	52	195	59	186	3.21e-18	79.0
MS.gene89612.t1	AT3G27830	38.194	144	73	1	52	195	63	190	3.56e-18	79.0
MS.gene89612.t1	AT3G27830	38.194	144	73	1	52	195	63	190	3.56e-18	79.0
MS.gene89612.t1	AT2G03130	49.438	89	41	1	108	196	7	91	5.90e-16	70.9
MS.gene89612.t1	AT3G27840	30.682	176	104	2	23	195	32	192	2.27e-15	71.2

Find 52 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTTGGTGAGAAAGGAAATT+AGG	0.194585	8.3:-27335907	None:intergenic
TGAAGTGATGCGAGAGAAAA+AGG	0.248644	8.3:+27336071	MS.gene89612:CDS
TGGTTCCTGGAATGGGAATA+AGG	0.260154	8.3:+27336124	MS.gene89612:CDS
TGATGCGGCGTCGAAGATTA+AGG	0.265836	8.3:+27336257	MS.gene89612:CDS
AGTTGGTGCTAAAGCTTCTA+TGG	0.325399	8.3:+27336413	MS.gene89612:CDS
AGGTGTAGGTGGAGGGAAAG+GGG	0.337630	8.3:+27336158	MS.gene89612:CDS
TTTGGTGAGAAAGGAAATTA+GGG	0.344047	8.3:-27335906	None:intergenic
TGCGGAGATGTGAGAATTGT+AGG	0.346963	8.3:-27335867	None:intergenic
TTAAGGTTATTAAAGAAGTT+AGG	0.362138	8.3:+27336274	MS.gene89612:CDS
GGTTCCTGGAATGGGAATAA+GGG	0.368867	8.3:+27336125	MS.gene89612:CDS
GCGTTTGATGTGAAGCTTGA+TGG	0.371046	8.3:+27336231	MS.gene89612:CDS
ATTCCCCTTATTCCCATTCC+AGG	0.371516	8.3:-27336129	None:intergenic
ATTATGATGCTGATGGTTCC+TGG	0.396685	8.3:+27336111	MS.gene89612:CDS
GGTGTAGGTGGAGGGAAAGG+GGG	0.396694	8.3:+27336159	MS.gene89612:CDS
AGAGGTGTAGGTGGAGGGAA+AGG	0.431861	8.3:+27336156	MS.gene89612:CDS
GTTGAAGGAAGCTAAGGATT+TGG	0.435359	8.3:+27336314	MS.gene89612:CDS
ATTGCTAAGTTGAAGGAAGT+TGG	0.461667	8.3:+27336396	MS.gene89612:CDS
GGAACCATCAGCATCATAAT+CGG	0.467185	8.3:-27336108	None:intergenic
GGGGATTATTGCTAAGTTGA+AGG	0.470094	8.3:+27336389	MS.gene89612:CDS
GAGGTGTAGGTGGAGGGAAA+GGG	0.479166	8.3:+27336157	MS.gene89612:CDS
TATGGAGGTGGATTTGCATT+GGG	0.481396	8.3:-27335950	None:intergenic
GTATGGAGGTGGATTTGCAT+TGG	0.489491	8.3:-27335951	None:intergenic
TTTGCATTGGGAGAGGATGC+AGG	0.489536	8.3:-27335938	None:intergenic
ATGCCGAGAGGTGTAGGTGG+AGG	0.492930	8.3:+27336150	MS.gene89612:CDS
ATGCTGATGGTTCCTGGAAT+GGG	0.503308	8.3:+27336117	MS.gene89612:CDS
TGTGACGAAGGAAGAGGCCG+AGG	0.503668	8.3:+27336368	MS.gene89612:CDS
TTCGGGATTCGGTGAATCGG+TGG	0.512716	8.3:-27335986	None:intergenic
TTTGGGGTTGAAGGAAGCTA+AGG	0.515112	8.3:+27336308	MS.gene89612:CDS
GATGCTGATGGTTCCTGGAA+TGG	0.517030	8.3:+27336116	MS.gene89612:CDS
GGTGGTGTAATTGCGGCGTA+TGG	0.529938	8.3:-27335968	None:intergenic
GTAATTGCGGCGTATGGAGG+TGG	0.535225	8.3:-27335962	None:intergenic
AGGGGAATGCCGAGAGGTGT+AGG	0.541839	8.3:+27336144	MS.gene89612:CDS
AGGTGGATTTGCATTGGGAG+AGG	0.544913	8.3:-27335945	None:intergenic
GAAGGAAGCTAAGGATTTGG+TGG	0.548600	8.3:+27336317	MS.gene89612:CDS
CAACTTAGCAATAATCCCCT+CGG	0.570601	8.3:-27336385	None:intergenic
GGGTGAGGTGAAGAAAGTTG+AGG	0.571665	8.3:+27336200	MS.gene89612:CDS
GAAAGGTGTGACGAAGGAAG+AGG	0.571984	8.3:+27336362	MS.gene89612:CDS
GGTGTAATTGCGGCGTATGG+AGG	0.572681	8.3:-27335965	None:intergenic
TTCCCTCCACCTACACCTCT+CGG	0.576289	8.3:-27336153	None:intergenic
TGCCGAGAGGTGTAGGTGGA+GGG	0.581642	8.3:+27336151	MS.gene89612:CDS
ATTAACGCTTCTCGAAGCAA+TGG	0.603358	8.3:+27336041	MS.gene89612:CDS
GTGAATCGGTGGTGTAATTG+CGG	0.609756	8.3:-27335975	None:intergenic
GTGACGAAGGAAGAGGCCGA+GGG	0.612413	8.3:+27336369	MS.gene89612:CDS
GGAATGCCGAGAGGTGTAGG+TGG	0.612707	8.3:+27336147	MS.gene89612:CDS
GCTTCCGATTATGATGCTGA+TGG	0.621281	8.3:+27336104	MS.gene89612:CDS
GCAATCGTCGACGAACTCGC+GGG	0.626410	8.3:+27336018	MS.gene89612:CDS
ACACCTTTCTTCAAAAGCGT+AGG	0.629486	8.3:-27336348	None:intergenic
AGCAATCGTCGACGAACTCG+CGG	0.632941	8.3:+27336017	MS.gene89612:CDS
GTTCCTGGAATGGGAATAAG+GGG	0.637463	8.3:+27336126	MS.gene89612:CDS
TTTGAAGAAAGGTGTGACGA+AGG	0.649602	8.3:+27336356	MS.gene89612:CDS
TGACGAAGGAAGAGGCCGAG+GGG	0.703464	8.3:+27336370	MS.gene89612:CDS
GGAATAAGGGGAATGCCGAG+AGG	0.729602	8.3:+27336138	MS.gene89612:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAGGTTATTAAAGAAGTT+AGG	+	chr8.3:27336274-27336293	MS.gene89612:CDS	20.0%
!!!	GAAATTAGGGTTTTGAATTA+GGG	-	chr8.3:27335896-27335915	None:intergenic	25.0%
	TTTGGTGAGAAAGGAAATTA+GGG	-	chr8.3:27335909-27335928	None:intergenic	30.0%
!	AGTTAGGAGTTTTACTAGTT+TGG	+	chr8.3:27336290-27336309	MS.gene89612:CDS	30.0%
!	ATTTGGTGAGAAAGGAAATT+AGG	-	chr8.3:27335910-27335929	None:intergenic	30.0%
!!	GTTAGGAGTTTTACTAGTTT+GGG	+	chr8.3:27336291-27336310	MS.gene89612:CDS	30.0%
!!	TTAGGAGTTTTACTAGTTTG+GGG	+	chr8.3:27336292-27336311	MS.gene89612:CDS	30.0%
!!!	GGAAATTAGGGTTTTGAATT+AGG	-	chr8.3:27335897-27335916	None:intergenic	30.0%
!!!	TTTTTGGAATTTGGTGAGAA+AGG	-	chr8.3:27335918-27335937	None:intergenic	30.0%
!	ATTGCTAAGTTGAAGGAAGT+TGG	+	chr8.3:27336396-27336415	MS.gene89612:CDS	35.0%
!!	GACGATTGCTGATACTTTTT+CGG	-	chr8.3:27336007-27336026	None:intergenic	35.0%
!!	TTTTACTAGTTTGGGGTTGA+AGG	+	chr8.3:27336299-27336318	MS.gene89612:CDS	35.0%
!!!	ACGATTGCTGATACTTTTTC+GGG	-	chr8.3:27336006-27336025	None:intergenic	35.0%
!!!	GGTTTTGAATTAGGGTTTTG+CGG	-	chr8.3:27335888-27335907	None:intergenic	35.0%
	ATTAACGCTTCTCGAAGCAA+TGG	+	chr8.3:27336041-27336060	MS.gene89612:CDS	40.0%
	ATTATGATGCTGATGGTTCC+TGG	+	chr8.3:27336111-27336130	MS.gene89612:CDS	40.0%
	CAACTTAGCAATAATCCCCT+CGG	-	chr8.3:27336388-27336407	None:intergenic	40.0%
	GAAGTGATGCGAGAGAAAAA+GGG	+	chr8.3:27336072-27336091	MS.gene89612:CDS	40.0%
	GGAACCATCAGCATCATAAT+CGG	-	chr8.3:27336111-27336130	None:intergenic	40.0%
	GTTGAAGGAAGCTAAGGATT+TGG	+	chr8.3:27336314-27336333	MS.gene89612:CDS	40.0%
	TGAAGTGATGCGAGAGAAAA+AGG	+	chr8.3:27336071-27336090	MS.gene89612:CDS	40.0%
!	ACACCTTTCTTCAAAAGCGT+AGG	-	chr8.3:27336351-27336370	None:intergenic	40.0%
!	GGGGATTATTGCTAAGTTGA+AGG	+	chr8.3:27336389-27336408	MS.gene89612:CDS	40.0%
!	TATGGAGGTGGATTTGCATT+GGG	-	chr8.3:27335953-27335972	None:intergenic	40.0%
!	TTTGAAGAAAGGTGTGACGA+AGG	+	chr8.3:27336356-27336375	MS.gene89612:CDS	40.0%
!!	AGTTGGTGCTAAAGCTTCTA+TGG	+	chr8.3:27336413-27336432	MS.gene89612:CDS	40.0%
!!!	GAAGCTTGATGGTTTTGATG+CGG	+	chr8.3:27336242-27336261	MS.gene89612:CDS	40.0%
!!!	GCTGATACTTTTTCGGGATT+CGG	-	chr8.3:27336000-27336019	None:intergenic	40.0%
!!!	GGATGCAGGTTTTTGGAATT+TGG	-	chr8.3:27335927-27335946	None:intergenic	40.0%
!!!	TTTTTCGGGATTCGGTGAAT+CGG	-	chr8.3:27335992-27336011	None:intergenic	40.0%
	ATGCTGATGGTTCCTGGAAT+GGG	+	chr8.3:27336117-27336136	MS.gene89612:CDS	45.0%
	ATTCCCCTTATTCCCATTCC+AGG	-	chr8.3:27336132-27336151	None:intergenic	45.0%
	GAAGGAAGCTAAGGATTTGG+TGG	+	chr8.3:27336317-27336336	MS.gene89612:CDS	45.0%
	GCGTTTGATGTGAAGCTTGA+TGG	+	chr8.3:27336231-27336250	MS.gene89612:CDS	45.0%
	GCTTCCGATTATGATGCTGA+TGG	+	chr8.3:27336104-27336123	MS.gene89612:CDS	45.0%
	GGTTCCTGGAATGGGAATAA+GGG	+	chr8.3:27336125-27336144	MS.gene89612:CDS	45.0%
	GTGAATCGGTGGTGTAATTG+CGG	-	chr8.3:27335978-27335997	None:intergenic	45.0%
	GTTCCTGGAATGGGAATAAG+GGG	+	chr8.3:27336126-27336145	MS.gene89612:CDS	45.0%
	TGCGGAGATGTGAGAATTGT+AGG	-	chr8.3:27335870-27335889	None:intergenic	45.0%
	TGGTTCCTGGAATGGGAATA+AGG	+	chr8.3:27336124-27336143	MS.gene89612:CDS	45.0%
!	GTATGGAGGTGGATTTGCAT+TGG	-	chr8.3:27335954-27335973	None:intergenic	45.0%
!	TTTGGGGTTGAAGGAAGCTA+AGG	+	chr8.3:27336308-27336327	MS.gene89612:CDS	45.0%
!!	TCGCCTACGCTTTTGAAGAA+AGG	+	chr8.3:27336345-27336364	MS.gene89612:CDS	45.0%
	GATGCTGATGGTTCCTGGAA+TGG	+	chr8.3:27336116-27336135	MS.gene89612:CDS	50.0%
	GGGTGAGGTGAAGAAAGTTG+AGG	+	chr8.3:27336200-27336219	MS.gene89612:CDS	50.0%
	TTTGCATTGGGAGAGGATGC+AGG	-	chr8.3:27335941-27335960	None:intergenic	50.0%
!	AGGTGGATTTGCATTGGGAG+AGG	-	chr8.3:27335948-27335967	None:intergenic	50.0%
!	GAAAGGTGTGACGAAGGAAG+AGG	+	chr8.3:27336362-27336381	MS.gene89612:CDS	50.0%
!	GGGGTGGTGGTAGTGAAAAA+GGG	+	chr8.3:27336178-27336197	MS.gene89612:CDS	50.0%
!	GGGTGGTGGTAGTGAAAAAG+GGG	+	chr8.3:27336179-27336198	MS.gene89612:CDS	50.0%
!	GGTGGTGGTAGTGAAAAAGG+GGG	+	chr8.3:27336180-27336199	MS.gene89612:CDS	50.0%
!	TGATGCGGCGTCGAAGATTA+AGG	+	chr8.3:27336257-27336276	MS.gene89612:CDS	50.0%
!	TGGGAGAGGATGCAGGTTTT+TGG	-	chr8.3:27335934-27335953	None:intergenic	50.0%
!	TGGTAGTGAAAAAGGGGGTG+AGG	+	chr8.3:27336185-27336204	MS.gene89612:CDS	50.0%
	AGCAATCGTCGACGAACTCG+CGG	+	chr8.3:27336017-27336036	MS.gene89612:CDS	55.0%
	AGGTGTAGGTGGAGGGAAAG+GGG	+	chr8.3:27336158-27336177	MS.gene89612:CDS	55.0%
	GAGGTGTAGGTGGAGGGAAA+GGG	+	chr8.3:27336157-27336176	MS.gene89612:CDS	55.0%
	GGAATAAGGGGAATGCCGAG+AGG	+	chr8.3:27336138-27336157	MS.gene89612:CDS	55.0%
	GGTGGTGTAATTGCGGCGTA+TGG	-	chr8.3:27335971-27335990	None:intergenic	55.0%
	GGTGTAATTGCGGCGTATGG+AGG	-	chr8.3:27335968-27335987	None:intergenic	55.0%
	GTAATTGCGGCGTATGGAGG+TGG	-	chr8.3:27335965-27335984	None:intergenic	55.0%
	TTCCCTCCACCTACACCTCT+CGG	-	chr8.3:27336156-27336175	None:intergenic	55.0%
!	AGAGGTGTAGGTGGAGGGAA+AGG	+	chr8.3:27336156-27336175	MS.gene89612:CDS	55.0%
!	GGGGGTGGTGGTAGTGAAAA+AGG	+	chr8.3:27336177-27336196	MS.gene89612:CDS	55.0%
!!	TTCGGGATTCGGTGAATCGG+TGG	-	chr8.3:27335989-27336008	None:intergenic	55.0%
	GCAATCGTCGACGAACTCGC+GGG	+	chr8.3:27336018-27336037	MS.gene89612:CDS	60.0%
	GGTGTAGGTGGAGGGAAAGG+GGG	+	chr8.3:27336159-27336178	MS.gene89612:CDS	60.0%
	GTGACGAAGGAAGAGGCCGA+GGG	+	chr8.3:27336369-27336388	MS.gene89612:CDS	60.0%
	TGACGAAGGAAGAGGCCGAG+GGG	+	chr8.3:27336370-27336389	MS.gene89612:CDS	60.0%
	TGTGACGAAGGAAGAGGCCG+AGG	+	chr8.3:27336368-27336387	MS.gene89612:CDS	60.0%
!	AGGGGAATGCCGAGAGGTGT+AGG	+	chr8.3:27336144-27336163	MS.gene89612:CDS	60.0%
!	ATGCCGAGAGGTGTAGGTGG+AGG	+	chr8.3:27336150-27336169	MS.gene89612:CDS	60.0%
!	GGAATGCCGAGAGGTGTAGG+TGG	+	chr8.3:27336147-27336166	MS.gene89612:CDS	60.0%
!	TGCCGAGAGGTGTAGGTGGA+GGG	+	chr8.3:27336151-27336170	MS.gene89612:CDS	60.0%
	GTAGGTGGAGGGAAAGGGGG+TGG	+	chr8.3:27336162-27336181	MS.gene89612:CDS	65.0%
	GGTGGAGGGAAAGGGGGTGG+TGG	+	chr8.3:27336165-27336184	MS.gene89612:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	27335850	27336440	27335850	ID=MS.gene89612
chr8.3	mRNA	27335850	27336440	27335850	ID=MS.gene89612.t1;Parent=MS.gene89612
chr8.3	exon	27335850	27336440	27335850	ID=MS.gene89612.t1.exon1;Parent=MS.gene89612.t1
chr8.3	CDS	27335850	27336440	27335850	ID=cds.MS.gene89612.t1;Parent=MS.gene89612.t1

Gene Sequence

>MS.gene89612

ATGAAGTTTTCACGTTTCCTACAATTCTCACATCTCCGCAAAACCCTAATTCAAAACCCTAATTTCCTTTCTCACCAAATTCCAAAAACCTGCATCCTCTCCCAATGCAAATCCACCTCCATACGCCGCAATTACACCACCGATTCACCGAATCCCGAAAAAGTATCAGCAATCGTCGACGAACTCGCGGGATTAACGCTTCTCGAAGCAATGGATCTCGTTGAAGTGATGCGAGAGAAAAAGGGAATCAACGAGCTTCCGATTATGATGCTGATGGTTCCTGGAATGGGAATAAGGGGAATGCCGAGAGGTGTAGGTGGAGGGAAAGGGGGTGGTGGTAGTGAAAAAGGGGGTGAGGTGAAGAAAGTTGAGGAGAAAACTGCGTTTGATGTGAAGCTTGATGGTTTTGATGCGGCGTCGAAGATTAAGGTTATTAAAGAAGTTAGGAGTTTTACTAGTTTGGGGTTGAAGGAAGCTAAGGATTTGGTGGAGAAATCGCCTACGCTTTTGAAGAAAGGTGTGACGAAGGAAGAGGCCGAGGGGATTATTGCTAAGTTGAAGGAAGTTGGTGCTAAAGCTTCTATGGAATAA

Protein sequence

>MS.gene89612.t1

MKFSRFLQFSHLRKTLIQNPNFLSHQIPKTCILSQCKSTSIRRNYTTDSPNPEKVSAIVDELAGLTLLEAMDLVEVMREKKGINELPIMMLMVPGMGIRGMPRGVGGGKGGGGSEKGGEVKKVEEKTAFDVKLDGFDAASKIKVIKEVRSFTSLGLKEAKDLVEKSPTLLKKGVTKEEAEGIIAKLKEVGAKASME