Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89646.t1 | TKY55883.1 | 67.7 | 396 | 57 | 3 | 12 | 339 | 5 | 397 | 1.10E-143 | 519.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89646.t1 | Q9SL43 | 62.4 | 245 | 75 | 3 | 11 | 239 | 4 | 247 | 1.5e-80 | 301.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89646.t1 | A0A4D6L033 | 67.4 | 384 | 57 | 2 | 24 | 339 | 24 | 407 | 3.7e-138 | 500.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene89646 | MS.gene30166 | PPI |
| MS.gene89646 | MS.gene027087 | PPI |
| MS.gene89646 | MS.gene82727 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89646.t1 | MTR_8g076060 | 91.322 | 242 | 8 | 2 | 11 | 239 | 4 | 245 | 4.48e-154 | 432 |
| MS.gene89646.t1 | MTR_8g076060 | 94.595 | 185 | 9 | 1 | 11 | 194 | 4 | 188 | 6.35e-122 | 348 |
| MS.gene89646.t1 | MTR_8g076070 | 94.340 | 106 | 6 | 0 | 235 | 340 | 17 | 122 | 7.12e-68 | 209 |
| MS.gene89646.t1 | MTR_3g075380 | 42.763 | 152 | 82 | 2 | 46 | 193 | 17 | 167 | 5.32e-32 | 119 |
| MS.gene89646.t1 | MTR_3g051460 | 42.553 | 141 | 76 | 2 | 57 | 193 | 19 | 158 | 3.09e-27 | 105 |
| MS.gene89646.t1 | MTR_3g051460 | 42.553 | 141 | 76 | 2 | 57 | 193 | 36 | 175 | 3.86e-27 | 106 |
| MS.gene89646.t1 | MTR_3g051460 | 42.553 | 141 | 76 | 2 | 57 | 193 | 71 | 210 | 2.55e-26 | 105 |
| MS.gene89646.t1 | MTR_5g092680 | 43.262 | 141 | 75 | 2 | 57 | 193 | 36 | 175 | 3.36e-26 | 103 |
| MS.gene89646.t1 | MTR_5g092680 | 43.262 | 141 | 75 | 2 | 57 | 193 | 98 | 237 | 2.61e-25 | 103 |
| MS.gene89646.t1 | MTR_5g092680 | 43.262 | 141 | 75 | 2 | 57 | 193 | 99 | 238 | 2.66e-25 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89646.t1 | AT2G18030 | 62.449 | 245 | 75 | 3 | 11 | 239 | 4 | 247 | 3.62e-105 | 308 |
| MS.gene89646.t1 | AT2G18030 | 62.766 | 188 | 65 | 2 | 11 | 194 | 4 | 190 | 2.79e-80 | 243 |
| MS.gene89646.t1 | AT2G18040 | 73.451 | 113 | 30 | 0 | 227 | 339 | 6 | 118 | 7.36e-53 | 170 |
| MS.gene89646.t1 | AT5G61640 | 41.844 | 141 | 77 | 2 | 57 | 193 | 36 | 175 | 5.39e-28 | 108 |
| MS.gene89646.t1 | AT5G61640 | 41.844 | 141 | 77 | 2 | 57 | 193 | 63 | 202 | 9.96e-28 | 108 |
| MS.gene89646.t1 | AT5G07460 | 38.889 | 162 | 90 | 3 | 40 | 193 | 31 | 191 | 1.03e-26 | 105 |
| MS.gene89646.t1 | AT4G25130 | 43.262 | 141 | 75 | 2 | 57 | 193 | 92 | 231 | 3.91e-26 | 105 |
| MS.gene89646.t1 | AT5G07470 | 40.972 | 144 | 74 | 3 | 57 | 193 | 36 | 175 | 6.55e-25 | 100 |
Find 96 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTCGCTCTCGGAAGTTTC+TGG | 0.121780 | 8.3:-27801688 | MS.gene89646:CDS |
| ACGTTCCTTCTTCAGCTAAT+TGG | 0.182191 | 8.3:-27798236 | MS.gene89646:intron |
| ATGAAAGAGGATATCCTTTC+TGG | 0.182475 | 8.3:-27796373 | MS.gene89646:CDS |
| GGGAACTACTTGATATATTT+TGG | 0.185058 | 8.3:-27800271 | MS.gene89646:CDS |
| GATCCACGGTTAATTAGTTT+TGG | 0.241655 | 8.3:-27800293 | MS.gene89646:CDS |
| TCTTTGCATCAATTTGCTTT+TGG | 0.259160 | 8.3:+27798129 | None:intergenic |
| TTCTTGGCTGTAGGTCCTTT+TGG | 0.284324 | 8.3:-27793447 | MS.gene89646:intron |
| CGAAGCAAGATCCTCAAATT+TGG | 0.289474 | 8.3:+27796345 | None:intergenic |
| ACCTGGTGCTCAGGCTCTGC+CGG | 0.306819 | 8.3:+27799960 | None:intergenic |
| AATCCTGAATATCGTAGTTT+TGG | 0.314646 | 8.3:-27801590 | MS.gene89646:CDS |
| AAACTACGATATTCAGGATT+AGG | 0.317186 | 8.3:+27801593 | None:intergenic |
| CTTCAAAAGGCTTCTGCATC+TGG | 0.328903 | 8.3:+27793418 | None:intergenic |
| CGTTCCTTCTTCAGCTAATT+GGG | 0.331094 | 8.3:-27798235 | MS.gene89646:intron |
| CAAACACCTGCCTGGGATCA+TGG | 0.346817 | 8.3:+27800243 | None:intergenic |
| TTCTTGATGATGTAACCTTC+TGG | 0.351777 | 8.3:+27796433 | None:intergenic |
| TGGAGATCCGAAGCTGTGTT+CGG | 0.368348 | 8.3:-27801668 | MS.gene89646:CDS |
| GCTAAACGCGGTGGCGATCT+TGG | 0.368998 | 8.3:-27796301 | MS.gene89646:intron |
| AGGACCACAGTGGGTTATTC+CGG | 0.375850 | 8.3:-27801626 | MS.gene89646:CDS |
| TCACCAAAACTACGATATTC+AGG | 0.386102 | 8.3:+27801587 | None:intergenic |
| AATTTGTTCTCTTGATTATG+TGG | 0.388827 | 8.3:+27793334 | None:intergenic |
| GATCTCTCGAGCTCCTCTTC+AGG | 0.389871 | 8.3:+27798206 | None:intergenic |
| TGATCACGCTGAATCTGTTC+AGG | 0.393265 | 8.3:-27801567 | MS.gene89646:intron |
| GCCTTGGCCAAACACCTGCC+TGG | 0.393908 | 8.3:+27800235 | None:intergenic |
| CACAATGCTGCTTCTTGACC+TGG | 0.394204 | 8.3:+27800016 | None:intergenic |
| TAGCGATCCGATCCGGGAAT+CGG | 0.404498 | 8.3:+27801756 | None:intergenic |
| GGTTTGCAAGCGCTGTTGAT+CGG | 0.405880 | 8.3:+27801715 | None:intergenic |
| CATGATCCCAGGCAGGTGTT+TGG | 0.406390 | 8.3:-27800242 | MS.gene89646:CDS |
| TGCAAACTGTATCCGATTCC+CGG | 0.410898 | 8.3:-27801768 | MS.gene89646:CDS |
| GGAGATCCGAAGCTGTGTTC+GGG | 0.416814 | 8.3:-27801667 | MS.gene89646:CDS |
| GCGGGTTTAGCGATCCGATC+CGG | 0.420813 | 8.3:+27801749 | None:intergenic |
| CCCGGCAGAGCCTGAGCACC+AGG | 0.421997 | 8.3:-27799961 | MS.gene89646:intron |
| GTAGAGAATGTAGTTATAGT+TGG | 0.423908 | 8.3:+27801826 | None:intergenic |
| GTTTGCAAGCGCTGTTGATC+GGG | 0.425301 | 8.3:+27801716 | None:intergenic |
| TTCTGGTAAGGCCAAATTTG+AGG | 0.428366 | 8.3:-27796356 | MS.gene89646:CDS |
| GGAGACGGTGGGTGCTAGAA+TGG | 0.432957 | 8.3:-27802639 | MS.gene89646:CDS |
| CTGTACTGATTACCCACATC+AGG | 0.433767 | 8.3:+27800212 | None:intergenic |
| GGCTTCTGTCAGCAAAGAAC+AGG | 0.442027 | 8.3:-27800045 | MS.gene89646:CDS |
| GAAAGTCAAACCTGGTGCTC+AGG | 0.442914 | 8.3:+27799951 | None:intergenic |
| CCCAGGCAGGTGTTTGGCCA+AGG | 0.443504 | 8.3:-27800236 | MS.gene89646:CDS |
| CAAATTTGGCCTTACCAGAA+AGG | 0.448271 | 8.3:+27796359 | None:intergenic |
| TATTCTTACTTCAATGAAAG+AGG | 0.454286 | 8.3:-27796386 | MS.gene89646:CDS |
| GCATAAGTTGCATCTTCAAA+AGG | 0.464113 | 8.3:+27793405 | None:intergenic |
| TGCATCTGGCCACGACCAAA+AGG | 0.471277 | 8.3:+27793432 | None:intergenic |
| TGATCGGGTTGTTCGCGGGC+GGG | 0.472390 | 8.3:+27801731 | None:intergenic |
| CCTTGGCCAAACACCTGCCT+GGG | 0.485328 | 8.3:+27800236 | None:intergenic |
| GCTGTTGATCGGGTTGTTCG+CGG | 0.492191 | 8.3:+27801726 | None:intergenic |
| CTGTTGATCGGGTTGTTCGC+GGG | 0.492272 | 8.3:+27801727 | None:intergenic |
| GCTGTGTTCGGGTGTTTACC+TGG | 0.492300 | 8.3:-27801656 | MS.gene89646:CDS |
| GCAACTTATGCTCTTCAAGT+TGG | 0.495197 | 8.3:-27793393 | MS.gene89646:CDS |
| ACTGTATCCGATTCCCGGAT+CGG | 0.505867 | 8.3:-27801763 | MS.gene89646:CDS |
| CGGGTTTAGCGATCCGATCC+GGG | 0.508862 | 8.3:+27801750 | None:intergenic |
| AACCTGGCAACAAAACTGAA+TGG | 0.510146 | 8.3:-27798182 | MS.gene89646:CDS |
| ATTTGTTCTCTTGATTATGT+GGG | 0.511033 | 8.3:+27793335 | None:intergenic |
| ACTACTCAAATTCTGCAACT+TGG | 0.511065 | 8.3:-27799993 | MS.gene89646:CDS |
| AGGATTAGGTTTAGATCCAC+CGG | 0.512586 | 8.3:+27801607 | None:intergenic |
| CCTGGTGCTCAGGCTCTGCC+GGG | 0.514034 | 8.3:+27799961 | None:intergenic |
| TTTGGTCAAGCCATGATCCC+AGG | 0.532861 | 8.3:-27800253 | MS.gene89646:CDS |
| AGAGGATATCCTTTCTGGTA+AGG | 0.533949 | 8.3:-27796368 | MS.gene89646:CDS |
| GCAAAGAACAGGAGCAAACC+AGG | 0.534889 | 8.3:-27800034 | MS.gene89646:CDS |
| GAGACGGTGGGTGCTAGAAT+GGG | 0.535823 | 8.3:-27802638 | MS.gene89646:CDS |
| TGGAACTGAAGAATCAAGAA+TGG | 0.536435 | 8.3:-27800066 | MS.gene89646:CDS |
| TTGATCGGGTTGTTCGCGGG+CGG | 0.540227 | 8.3:+27801730 | None:intergenic |
| TAAACACCCGAACACAGCTT+CGG | 0.544459 | 8.3:+27801661 | None:intergenic |
| TCTCGAGCTCCTCTTCAGGC+AGG | 0.547751 | 8.3:+27798210 | None:intergenic |
| AATTTGAGGATCTTGCTTCG+AGG | 0.550226 | 8.3:-27796342 | MS.gene89646:CDS |
| GATTACCCACATCAGGGCCT+TGG | 0.551890 | 8.3:+27800219 | None:intergenic |
| CTGATGTGGGTAATCAGTAC+AGG | 0.553146 | 8.3:-27800211 | MS.gene89646:intron |
| GTTCTAGAAGAAAAGCTTCG+TGG | 0.556909 | 8.3:-27796462 | MS.gene89646:CDS |
| GGAGCTCGAGAGATCAAACC+TGG | 0.562118 | 8.3:-27798198 | MS.gene89646:CDS |
| GTCAAGCCATGATCCCAGGC+AGG | 0.571161 | 8.3:-27800249 | MS.gene89646:CDS |
| GTTTGGCCAAGGCCCTGATG+TGG | 0.577488 | 8.3:-27800225 | MS.gene89646:CDS |
| ACTGTGGTCCTCACAACACC+AGG | 0.580105 | 8.3:+27801638 | None:intergenic |
| AATTGGGAACCTGCCTGAAG+AGG | 0.585218 | 8.3:-27798219 | MS.gene89646:CDS |
| TTTGGCCAAGGCCCTGATGT+GGG | 0.588131 | 8.3:-27800224 | MS.gene89646:CDS |
| TGGTGAAATTAGTGACATTG+TGG | 0.589271 | 8.3:-27793373 | MS.gene89646:CDS |
| CATTGAAGTAAGAATAGCAA+CGG | 0.597353 | 8.3:+27796393 | None:intergenic |
| CAAACCGCGGTATTCGCTCT+CGG | 0.597580 | 8.3:-27801698 | MS.gene89646:CDS |
| GTGAATTATGATGGAGACGG+TGG | 0.608610 | 8.3:-27802651 | None:intergenic |
| GTGGGTGCTAGAATGGGTAG+TGG | 0.618736 | 8.3:-27802632 | MS.gene89646:intron |
| ACCACAGTGGGTTATTCCGG+TGG | 0.627927 | 8.3:-27801623 | MS.gene89646:CDS |
| GGTGTTTACCTGGTGTTGTG+AGG | 0.638048 | 8.3:-27801646 | MS.gene89646:CDS |
| GCAACTTGGAACATTTCACC+CGG | 0.641918 | 8.3:-27799979 | MS.gene89646:CDS |
| TGTACTGATTACCCACATCA+GGG | 0.643763 | 8.3:+27800213 | None:intergenic |
| TCCCCAAAACTAATTAACCG+TGG | 0.658842 | 8.3:+27800290 | None:intergenic |
| TTCAGGTTGAGTATGATCCA+CGG | 0.660705 | 8.3:-27800307 | MS.gene89646:intron |
| GACATTGTGGATAGTGACAG+TGG | 0.676092 | 8.3:-27793360 | MS.gene89646:CDS |
| TGTAGCTCTGCTAAACGCGG+TGG | 0.678782 | 8.3:-27796310 | MS.gene89646:CDS |
| GATTGTAGCTCTGCTAAACG+CGG | 0.692633 | 8.3:-27796313 | MS.gene89646:CDS |
| TCAACAGCGCTTGCAAACCG+CGG | 0.697314 | 8.3:-27801711 | MS.gene89646:CDS |
| TGAATTATGATGGAGACGGT+GGG | 0.703984 | 8.3:-27802650 | None:intergenic |
| GCTTCGTGGAAAGATCCAGA+AGG | 0.706542 | 8.3:-27796448 | MS.gene89646:CDS |
| ACTTCCGAGAGCGAATACCG+CGG | 0.721213 | 8.3:+27801694 | None:intergenic |
| GGTGTTGTGAGGACCACAGT+GGG | 0.732450 | 8.3:-27801635 | MS.gene89646:CDS |
| GAAGTGAATTATGATGGAGA+CGG | 0.747887 | 8.3:-27802654 | None:intergenic |
| TGGTGTTGTGAGGACCACAG+TGG | 0.758918 | 8.3:-27801636 | MS.gene89646:CDS |
| TCCACCGGAATAACCCACTG+TGG | 0.773637 | 8.3:+27801622 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 27793332 | 27802666 | 27793332 | ID=MS.gene89646 |
| chr8.3 | mRNA | 27793332 | 27802666 | 27793332 | ID=MS.gene89646.t1;Parent=MS.gene89646 |
| chr8.3 | exon | 27802633 | 27802666 | 27802633 | ID=MS.gene89646.t1.exon1;Parent=MS.gene89646.t1 |
| chr8.3 | CDS | 27802633 | 27802666 | 27802633 | ID=cds.MS.gene89646.t1;Parent=MS.gene89646.t1 |
| chr8.3 | exon | 27801568 | 27801854 | 27801568 | ID=MS.gene89646.t1.exon2;Parent=MS.gene89646.t1 |
| chr8.3 | CDS | 27801568 | 27801854 | 27801568 | ID=cds.MS.gene89646.t1;Parent=MS.gene89646.t1 |
| chr8.3 | exon | 27800212 | 27800324 | 27800212 | ID=MS.gene89646.t1.exon3;Parent=MS.gene89646.t1 |
| chr8.3 | CDS | 27800212 | 27800324 | 27800212 | ID=cds.MS.gene89646.t1;Parent=MS.gene89646.t1 |
| chr8.3 | exon | 27799962 | 27800106 | 27799962 | ID=MS.gene89646.t1.exon4;Parent=MS.gene89646.t1 |
| chr8.3 | CDS | 27799962 | 27800106 | 27799962 | ID=cds.MS.gene89646.t1;Parent=MS.gene89646.t1 |
| chr8.3 | exon | 27798108 | 27798243 | 27798108 | ID=MS.gene89646.t1.exon5;Parent=MS.gene89646.t1 |
| chr8.3 | CDS | 27798108 | 27798243 | 27798108 | ID=cds.MS.gene89646.t1;Parent=MS.gene89646.t1 |
| chr8.3 | exon | 27796302 | 27796484 | 27796302 | ID=MS.gene89646.t1.exon6;Parent=MS.gene89646.t1 |
| chr8.3 | CDS | 27796302 | 27796484 | 27796302 | ID=cds.MS.gene89646.t1;Parent=MS.gene89646.t1 |
| chr8.3 | exon | 27793332 | 27793456 | 27793332 | ID=MS.gene89646.t1.exon7;Parent=MS.gene89646.t1 |
| chr8.3 | CDS | 27793332 | 27793456 | 27793332 | ID=cds.MS.gene89646.t1;Parent=MS.gene89646.t1 |
| Gene Sequence |
| Protein sequence |
