AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90172


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90172.t1 MTR_5g030070 98.885 269 3 0 1 269 1 269 0.0 546
MS.gene90172.t1 MTR_8g066790 75.093 269 67 0 1 269 1 269 2.78e-151 423
MS.gene90172.t1 MTR_8g101600 50.373 268 133 0 1 268 1 268 8.19e-100 293
MS.gene90172.t1 MTR_4g061010 45.956 272 141 3 1 266 1 272 2.12e-78 239
MS.gene90172.t1 MTR_4g129670 45.318 267 142 3 1 264 1 266 3.84e-76 232
MS.gene90172.t1 MTR_1g095530 41.445 263 144 3 4 266 5 257 2.09e-66 208
MS.gene90172.t1 MTR_6g033335 41.016 256 141 3 4 259 5 250 2.27e-66 208
MS.gene90172.t1 MTR_4g112570 34.231 260 168 2 3 259 6 265 6.97e-54 176
MS.gene90172.t1 MTR_8g097280 31.984 247 159 4 1 244 3 243 7.28e-49 163
MS.gene90172.t1 MTR_2g056130 31.250 256 159 3 3 256 4 244 5.01e-44 150
MS.gene90172.t1 MTR_5g097460 28.458 253 155 6 5 252 50 281 1.38e-29 114
MS.gene90172.t1 MTR_8g097250 28.866 97 64 2 119 212 28 122 7.32e-12 61.6
MS.gene90172.t1 MTR_5g097450 30.233 129 66 4 118 241 84 193 1.23e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90172.t1 AT5G46700 67.286 269 88 0 1 269 1 269 2.13e-128 365
MS.gene90172.t1 AT2G19580 57.249 269 114 1 1 268 1 269 1.65e-100 295
MS.gene90172.t1 AT3G45600 44.528 265 141 3 1 259 1 265 1.75e-81 247
MS.gene90172.t1 AT4G28050 42.636 258 146 1 4 259 5 262 4.27e-78 237
MS.gene90172.t1 AT4G30430 41.603 262 151 1 3 262 4 265 2.35e-76 233
MS.gene90172.t1 AT2G23810 42.966 263 147 2 4 263 5 267 3.11e-76 233
MS.gene90172.t1 AT5G60220 43.893 262 141 3 1 256 1 262 2.04e-63 202
MS.gene90172.t1 AT1G18520 38.550 262 153 4 3 257 4 264 5.14e-61 194
MS.gene90172.t1 AT3G12090 40.316 253 139 4 4 256 5 245 1.29e-58 188
MS.gene90172.t1 AT4G23410 37.066 259 152 3 3 261 4 251 3.13e-55 179
MS.gene90172.t1 AT1G63260 32.171 258 167 3 1 257 3 253 2.74e-54 176
MS.gene90172.t1 AT1G63260 32.510 243 162 2 1 242 3 244 3.36e-51 169
MS.gene90172.t1 AT1G63260 32.510 243 162 2 1 242 3 244 3.36e-51 169
MS.gene90172.t1 AT1G63260 32.510 243 162 2 1 242 3 244 4.40e-51 169
MS.gene90172.t1 AT1G63260 32.719 217 144 2 1 216 3 218 3.48e-46 154
MS.gene90172.t1 AT3G45600 43.275 171 92 2 1 166 1 171 2.99e-43 146
MS.gene90172.t1 AT2G03840 29.707 239 160 4 19 251 32 268 1.40e-37 134
MS.gene90172.t1 AT1G63260 30.337 178 123 1 1 178 3 179 2.22e-33 121
MS.gene90172.t1 AT5G23030 27.132 258 183 2 1 256 2 256 2.92e-31 117
MS.gene90172.t1 AT5G57810 26.316 133 82 1 131 247 173 305 4.43e-16 77.0

Find 47 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACCTAATAGGGCTTATT+TGG 0.104253 5.1:+20663448 MS.gene90172:CDS
CTGGTCTCATTGGTTCCTTT+TGG 0.214283 5.1:+20663312 MS.gene90172:CDS
CTCTTAGTTGTGTCTCTTGC+TGG 0.223492 5.1:+20663293 MS.gene90172:CDS
ATGTCTGCTGCATTGTTTAT+TGG 0.243435 5.1:-20664048 None:intergenic
TATTATCTTGCTAGTTTGTT+TGG 0.252856 5.1:+20663382 MS.gene90172:CDS
TAGCAACTAGGTAGAATATT+AGG 0.309164 5.1:-20663349 None:intergenic
TATTCCAAATAAGCCCTATT+AGG 0.318593 5.1:-20663452 None:intergenic
TTAATTGTGGTTTATTTGAT+TGG 0.318944 5.1:+20664204 MS.gene90172:CDS
CAATCATTGGTGCTGGAATC+TGG 0.324358 5.1:+20663201 MS.gene90172:CDS
ATGGTATGATTGAACCTAAT+AGG 0.329364 5.1:+20663438 MS.gene90172:CDS
GCGAAAGAGATCCTCAGTTT+TGG 0.353207 5.1:-20664247 None:intergenic
GTGTCTCTTGCTGGTCTCAT+TGG 0.354014 5.1:+20663302 MS.gene90172:CDS
GGCTTATTTGGAATACCGTT+TGG 0.361064 5.1:+20663460 MS.gene90172:CDS
CTGGTTTGTTGGCAAATCTT+AGG 0.375317 5.1:+20664133 MS.gene90172:CDS
CATTTGTGAACCCAACATAC+TGG 0.397625 5.1:+20664019 MS.gene90172:CDS
TTGGACTAATCCAGTATGTT+GGG 0.406980 5.1:-20664029 None:intergenic
CAGATTCCAGCACCAATGAT+TGG 0.408669 5.1:-20663200 None:intergenic
TGCATTATTTATACCTGCAT+TGG 0.425207 5.1:-20663629 None:intergenic
AGTTGTAACAAAGTTGTGTT+TGG 0.428684 5.1:-20664095 None:intergenic
AGATTCCAGCACCAATGATT+GGG 0.447069 5.1:-20663199 None:intergenic
TGGTATGATTGAACCTAATA+GGG 0.447157 5.1:+20663439 MS.gene90172:CDS
AACTGTGACTCATGCAAAGC+TGG 0.452176 5.1:+20664114 MS.gene90172:CDS
GCAATGAGCAACAACATCAT+AGG 0.463723 5.1:+20663143 MS.gene90172:CDS
ATTGGACTAATCCAGTATGT+TGG 0.468680 5.1:-20664030 None:intergenic
GTTTACATGGTTACTCTTCG+TGG 0.472729 5.1:+20663413 MS.gene90172:CDS
CATGTGTCAAGTTTCTTCAA+TGG 0.476338 5.1:+20663246 MS.gene90172:CDS
ATCCTCTCAATCCCAATCAT+TGG 0.477309 5.1:+20663188 MS.gene90172:CDS
CTCATGCAAAGCTGGTTTGT+TGG 0.480759 5.1:+20664122 MS.gene90172:CDS
TCAATCCCAATCATTGGTGC+TGG 0.481205 5.1:+20663194 MS.gene90172:CDS
ATGGTTACTCTTCGTGGTCA+TGG 0.481914 5.1:+20663419 MS.gene90172:CDS
CAGACATGGATTGTCTTCAA+TGG 0.495893 5.1:+20664064 MS.gene90172:CDS
CAATGGCCAGTCATAATCCT+AGG 0.501117 5.1:+20663263 MS.gene90172:CDS
TTGAGAGGATTACAGCTACT+AGG 0.501297 5.1:-20663175 None:intergenic
TGGCAAATCTTAGGAAGGAA+TGG 0.515362 5.1:+20664142 MS.gene90172:CDS
GAGACATGAGATTCTCCAAA+AGG 0.523414 5.1:-20663327 None:intergenic
CACCAATGATTGGGATTGAG+AGG 0.525563 5.1:-20663190 None:intergenic
CTCTTTCGCAAGTACAAACA+AGG 0.545612 5.1:+20664261 MS.gene90172:CDS
GTGTATGCACATTGTGTTGG+TGG 0.546354 5.1:-20663997 None:intergenic
TGCACGTCTTACACCAATGC+AGG 0.553446 5.1:+20663616 MS.gene90172:CDS
AATAAACAATGCAGCAGACA+TGG 0.558636 5.1:+20664050 MS.gene90172:CDS
TTTGTTGGCAAATCTTAGGA+AGG 0.565313 5.1:+20664137 MS.gene90172:CDS
AACTTGACACATGTGTCTGC+TGG 0.569579 5.1:-20663236 None:intergenic
TAAGCACAAGCATAGCAACT+AGG 0.591892 5.1:-20663361 None:intergenic
AGAACACCTAGGATTATGAC+TGG 0.617019 5.1:-20663269 None:intergenic
AATGTGTATGCACATTGTGT+TGG 0.656084 5.1:-20664000 None:intergenic
CAACTAAGAGAAGAACACCT+AGG 0.686330 5.1:-20663280 None:intergenic
CTTAGTGAAACAAATATGTG+TGG 0.704201 5.1:+20663551 MS.gene90172:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAATTTGTTTATTTTTTAT+TGG - chr5.1:20663863-20663882 None:intergenic 10.0%
!! AGACTGAAAAATAATTAAAG+GGG - chr5.1:20663965-20663984 None:intergenic 20.0%
!! CAGACTGAAAAATAATTAAA+GGG - chr5.1:20663966-20663985 None:intergenic 20.0%
!! TTAAAGGGGAAAAAAATTAT+TGG - chr5.1:20663951-20663970 None:intergenic 20.0%
!! TTAATTGTGGTTTATTTGAT+TGG + chr5.1:20664204-20664223 MS.gene90172:CDS 20.0%
!!! CTTTAATTATTTTTCAGTCT+GGG + chr5.1:20663965-20663984 MS.gene90172:intron 20.0%
!!! TTTGGTTATTTTTGTTTACA+TGG + chr5.1:20663400-20663419 MS.gene90172:CDS 20.0%
! CCAGACTGAAAAATAATTAA+AGG - chr5.1:20663967-20663986 None:intergenic 25.0%
!!! AGTTAGAAGTTCTTTTAAGT+GGG + chr5.1:20663511-20663530 MS.gene90172:CDS 25.0%
!!! CCTTTAATTATTTTTCAGTC+TGG + chr5.1:20663964-20663983 MS.gene90172:intron 25.0%
!!! TATTATCTTGCTAGTTTGTT+TGG + chr5.1:20663382-20663401 MS.gene90172:CDS 25.0%
AGTTGTAACAAAGTTGTGTT+TGG - chr5.1:20664098-20664117 None:intergenic 30.0%
ATGGTATGATTGAACCTAAT+AGG + chr5.1:20663438-20663457 MS.gene90172:CDS 30.0%
CTTAGTGAAACAAATATGTG+TGG + chr5.1:20663551-20663570 MS.gene90172:CDS 30.0%
TAGCAACTAGGTAGAATATT+AGG - chr5.1:20663352-20663371 None:intergenic 30.0%
TATTCCAAATAAGCCCTATT+AGG - chr5.1:20663455-20663474 None:intergenic 30.0%
TGCATTATTTATACCTGCAT+TGG - chr5.1:20663632-20663651 None:intergenic 30.0%
TGGTATGATTGAACCTAATA+GGG + chr5.1:20663439-20663458 MS.gene90172:CDS 30.0%
! GAGTTAGAAGTTCTTTTAAG+TGG + chr5.1:20663510-20663529 MS.gene90172:CDS 30.0%
! GTTGAATCAAAGTTTTCGTA+TGG + chr5.1:20663577-20663596 MS.gene90172:CDS 30.0%
!! GTGACATTGACTTAAAATCT+GGG + chr5.1:20663769-20663788 MS.gene90172:intron 30.0%
!!! CAGTGTTGTTGTTTTAATTG+TGG + chr5.1:20664191-20664210 MS.gene90172:CDS 30.0%
AATAAACAATGCAGCAGACA+TGG + chr5.1:20664050-20664069 MS.gene90172:CDS 35.0%
AATGTGTATGCACATTGTGT+TGG - chr5.1:20664003-20664022 None:intergenic 35.0%
ACCCAGAAAAATCATCCAAA+CGG - chr5.1:20663478-20663497 None:intergenic 35.0%
ATCTCAAGATAAACCCACTT+CGG + chr5.1:20663738-20663757 MS.gene90172:intron 35.0%
ATGTCTGCTGCATTGTTTAT+TGG - chr5.1:20664051-20664070 None:intergenic 35.0%
ATTGGACTAATCCAGTATGT+TGG - chr5.1:20664033-20664052 None:intergenic 35.0%
CATGTGTCAAGTTTCTTCAA+TGG + chr5.1:20663246-20663265 MS.gene90172:CDS 35.0%
TGAACCTAATAGGGCTTATT+TGG + chr5.1:20663448-20663467 MS.gene90172:CDS 35.0%
TTGGACTAATCCAGTATGTT+GGG - chr5.1:20664032-20664051 None:intergenic 35.0%
TTTGTTGGCAAATCTTAGGA+AGG + chr5.1:20664137-20664156 MS.gene90172:CDS 35.0%
! ACCGTTTGGATGATTTTTCT+GGG + chr5.1:20663474-20663493 MS.gene90172:CDS 35.0%
! GATCTGTATCTAGTTTCACT+CGG - chr5.1:20663700-20663719 None:intergenic 35.0%
! GTGTTTCTCTTTTAGATCTC+AGG + chr5.1:20663795-20663814 MS.gene90172:intron 35.0%
! TACCGTTTGGATGATTTTTC+TGG + chr5.1:20663473-20663492 MS.gene90172:CDS 35.0%
!! GGTGACATTGACTTAAAATC+TGG + chr5.1:20663768-20663787 MS.gene90172:intron 35.0%
!! TGATTGGTTGCTTTGCTTTT+AGG + chr5.1:20664220-20664239 MS.gene90172:CDS 35.0%
!! TTTTAGGAATGCCAAAACTG+AGG + chr5.1:20664236-20664255 MS.gene90172:CDS 35.0%
AGAACACCTAGGATTATGAC+TGG - chr5.1:20663272-20663291 None:intergenic 40.0%
AGATTCCAGCACCAATGATT+GGG - chr5.1:20663202-20663221 None:intergenic 40.0%
ATCCTCTCAATCCCAATCAT+TGG + chr5.1:20663188-20663207 MS.gene90172:CDS 40.0%
CAACTAAGAGAAGAACACCT+AGG - chr5.1:20663283-20663302 None:intergenic 40.0%
CATTTGTGAACCCAACATAC+TGG + chr5.1:20664019-20664038 MS.gene90172:CDS 40.0%
CTCTTTCGCAAGTACAAACA+AGG + chr5.1:20664261-20664280 MS.gene90172:CDS 40.0%
GAGACATGAGATTCTCCAAA+AGG - chr5.1:20663330-20663349 None:intergenic 40.0%
GCAATGAGCAACAACATCAT+AGG + chr5.1:20663143-20663162 MS.gene90172:CDS 40.0%
GTTTACATGGTTACTCTTCG+TGG + chr5.1:20663413-20663432 MS.gene90172:CDS 40.0%
TAAGCACAAGCATAGCAACT+AGG - chr5.1:20663364-20663383 None:intergenic 40.0%
TGGCAAATCTTAGGAAGGAA+TGG + chr5.1:20664142-20664161 MS.gene90172:CDS 40.0%
TTGAGAGGATTACAGCTACT+AGG - chr5.1:20663178-20663197 None:intergenic 40.0%
! CAGACATGGATTGTCTTCAA+TGG + chr5.1:20664064-20664083 MS.gene90172:CDS 40.0%
! GGCTTATTTGGAATACCGTT+TGG + chr5.1:20663460-20663479 MS.gene90172:CDS 40.0%
! TCAAAGTTTTCGTATGGCTC+AGG + chr5.1:20663583-20663602 MS.gene90172:CDS 40.0%
!! CTGGTTTGTTGGCAAATCTT+AGG + chr5.1:20664133-20664152 MS.gene90172:CDS 40.0%
AACTGTGACTCATGCAAAGC+TGG + chr5.1:20664114-20664133 MS.gene90172:CDS 45.0%
AACTTGACACATGTGTCTGC+TGG - chr5.1:20663239-20663258 None:intergenic 45.0%
ATGGTTACTCTTCGTGGTCA+TGG + chr5.1:20663419-20663438 MS.gene90172:CDS 45.0%
CAAGATAAACCCACTTCGGT+TGG + chr5.1:20663742-20663761 MS.gene90172:intron 45.0%
CAATGGCCAGTCATAATCCT+AGG + chr5.1:20663263-20663282 MS.gene90172:CDS 45.0%
CACCAATGATTGGGATTGAG+AGG - chr5.1:20663193-20663212 None:intergenic 45.0%
CAGATTCCAGCACCAATGAT+TGG - chr5.1:20663203-20663222 None:intergenic 45.0%
CTCTTAGTTGTGTCTCTTGC+TGG + chr5.1:20663293-20663312 MS.gene90172:CDS 45.0%
GTGTATGCACATTGTGTTGG+TGG - chr5.1:20664000-20664019 None:intergenic 45.0%
TCAATCCCAATCATTGGTGC+TGG + chr5.1:20663194-20663213 MS.gene90172:CDS 45.0%
! GCGAAAGAGATCCTCAGTTT+TGG - chr5.1:20664250-20664269 None:intergenic 45.0%
!! CAATCATTGGTGCTGGAATC+TGG + chr5.1:20663201-20663220 MS.gene90172:CDS 45.0%
!! CTCATGCAAAGCTGGTTTGT+TGG + chr5.1:20664122-20664141 MS.gene90172:CDS 45.0%
!!! CTGGTCTCATTGGTTCCTTT+TGG + chr5.1:20663312-20663331 MS.gene90172:CDS 45.0%
TGCACGTCTTACACCAATGC+AGG + chr5.1:20663616-20663635 MS.gene90172:CDS 50.0%
! GTGTCTCTTGCTGGTCTCAT+TGG + chr5.1:20663302-20663321 MS.gene90172:CDS 50.0%
! TAAACCCACTTCGGTTGGTC+TGG + chr5.1:20663747-20663766 MS.gene90172:intron 50.0%
GTCACCAGACCAACCGAAGT+GGG - chr5.1:20663754-20663773 None:intergenic 55.0%
TGTCACCAGACCAACCGAAG+TGG - chr5.1:20663755-20663774 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 20663140 20664293 20663140 ID=MS.gene90172
chr5.1 mRNA 20663140 20664293 20663140 ID=MS.gene90172.t1;Parent=MS.gene90172
chr5.1 exon 20663140 20663637 20663140 ID=MS.gene90172.t1.exon1;Parent=MS.gene90172.t1
chr5.1 CDS 20663140 20663637 20663140 ID=cds.MS.gene90172.t1;Parent=MS.gene90172.t1
chr5.1 exon 20663982 20664293 20663982 ID=MS.gene90172.t1.exon2;Parent=MS.gene90172.t1
chr5.1 CDS 20663982 20664293 20663982 ID=cds.MS.gene90172.t1;Parent=MS.gene90172.t1
Gene Sequence

>MS.gene90172

ATGGCAATGAGCAACAACATCATAGGATTCATAAACCTAGTAGCTGTAATCCTCTCAATCCCAATCATTGGTGCTGGAATCTGGCTAACAAACGAACCAGCAGACACATGTGTCAAGTTTCTTCAATGGCCAGTCATAATCCTAGGTGTTCTTCTCTTAGTTGTGTCTCTTGCTGGTCTCATTGGTTCCTTTTGGAGAATCTCATGTCTCCTAATATTCTACCTAGTTGCTATGCTTGTGCTTATTATCTTGCTAGTTTGTTTGGTTATTTTTGTTTACATGGTTACTCTTCGTGGTCATGGTATGATTGAACCTAATAGGGCTTATTTGGAATACCGTTTGGATGATTTTTCTGGGTTTTTGAAAAGAAGAGTTAGAAGTTCTTTTAAGTGGGATGCTATTAGAAGTTGTCTTAGTGAAACAAATATGTGTGGTGAGTTGAATCAAAGTTTTCGTATGGCTCAGGACTTCTTTAATGCACGTCTTACACCAATGCAGGTATAAATAATGCAGTTTTTTTAGTTTATTTTACACATTTTCTCTCTACTGTACTTGATCCGAGTGAAACTAGATACAGATCTTTAAGTAAGAAAGTAAGATCTCAAGATAAACCCACTTCGGTTGGTCTGGTGACATTGACTTAAAATCTGGGAGTGTGTTTCTCTTTTAGATCTCAGGTTCGATAAATGACTGAAAGAAAATTAGTTATTACTAGTATCACCAATAAAAAATAAACAAATTACTGTTTGATAATTAAAAAAAATTCACAAAAATAGTTTCTGAGTTTTCTTTACTTACATATATTTTGCCAATAATTTTTTTCCCCTTTAATTATTTTTCAGTCTGGGTGCTGCAAGCCACCAACACAATGTGCATACACATTTGTGAACCCAACATACTGGATTAGTCCAATAAACAATGCAGCAGACATGGATTGTCTTCAATGGAGCAATGACCAAACACAACTTTGTTACAACTGTGACTCATGCAAAGCTGGTTTGTTGGCAAATCTTAGGAAGGAATGGAAAAGAGCTAATGTGATATTGATCATCAGTGTTGTTGTTTTAATTGTGGTTTATTTGATTGGTTGCTTTGCTTTTAGGAATGCCAAAACTGAGGATCTCTTTCGCAAGTACAAACAAGGTTACACTTGA

Protein sequence

>MS.gene90172.t1

MAMSNNIIGFINLVAVILSIPIIGAGIWLTNEPADTCVKFLQWPVIILGVLLLVVSLAGLIGSFWRISCLLIFYLVAMLVLIILLVCLVIFVYMVTLRGHGMIEPNRAYLEYRLDDFSGFLKRRVRSSFKWDAIRSCLSETNMCGELNQSFRMAQDFFNARLTPMQSGCCKPPTQCAYTFVNPTYWISPINNAADMDCLQWSNDQTQLCYNCDSCKAGLLANLRKEWKRANVILIISVVVLIVVYLIGCFAFRNAKTEDLFRKYKQGYT